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A five-year cooperative research program has been carried out by China Institute for Radiation Protection and Japan Atomic Energy Research Institute to develop safety assessment methodology for disposal of low level radioactive waste. Migration behavior of radionuclides 3H and 85Sr through field tests, simulation tests in the laboratory and determination of distribution coefficients is discussed and analyzed in this paper. The results show that the retardation coefficients, Rd, from field tests are about 0.08−3.3 times those from simulation tests and Rd from batch tests are 1.1−44 times those from field tests for 85Sr and loess medium. It was observed from field tests that radionuclides moved mainly downward under artificial sprinkling and a part of them moved up besides downward under natural rain condition. In addition, it was discovered that the retardation coefficient, Rd , increases with velocity of unsaturated water flow, u, in the analysis.