Search

Your search keyword '"Guo, Xiaoxin"' showing total 286 results
Start Over Author "Guo, Xiaoxin"
286 results on '"Guo, Xiaoxin"'

6. Evidence of an Annexin A4 mediated plasma membrane repair response to biomechanical strain associated with glaucoma pathogenesis

Author

Vicic, Nevena, Guo, Xiaoxin, Chan, Darren, Flanagan, John G, Sigal, Ian A, and Sivak, Jeremy M

Subjects
Neurosciences, Neurodegenerative, Bioengineering, Aging, Aetiology, 2.1 Biological and endogenous factors, Neurological, Annexin A4, Calcium, Cell Membrane, Glaucoma, Humans, Proteomics, Annexins, ANXA4, astrocytes, biomechanics, glaucoma, optic nerve, plasma membrane repair, stretch, Biochemistry and Cell Biology, Medical Physiology, Biochemistry & Molecular Biology
Abstract

Glaucoma is a common neurodegenerative blinding disease that is closely associated with chronic biomechanical strain at the optic nerve head (ONH). Yet, the cellular injury and mechanosensing mechanisms underlying the resulting damage have remained critically unclear. We previously identified Annexin A4 (ANXA4) from a proteomic analyses of human ONH astrocytes undergoing pathological biomechanical strain that mimics glaucomatous conditions. Annexins are a family of calcium-dependent phospholipid binding proteins with key functions in plasma membrane repair (PMR); an active mechanism to limit and mend cellular injury that involves membrane and cytoskeletal reorganizations. However, a role for direct membrane damage and PMR has not been well studied in the context of biomechanical strain, such as that associated with glaucoma. Here we report that this moderate strain surprisingly damages cell membranes to increase permeability in a calcium-dependent manner, and induces rapid aggregation of ANXA4 at injury sites. ANXA4 loss-of-function increases permeability, while exogenous ANXA4 reduces it. Furthermore, ANXA4 aggregation is associated with F-actin dynamics in vitro, and remarkably this interaction and aggregation signature is also observed in the glaucomatous ONH in patient samples. Together these studies link moderate biomechanical strain with direct membrane damage and actin dynamics, and identify an active PMR role for ANXA4 in new model of cell injury associated with glaucoma pathogenesis.

Published
2022

32. Pharmacologic inhibition of reactive gliosis blocks TNF-α-mediated neuronal apoptosis.

Author

Livne-Bar, Izhar, Lam, Susy, Chan, Darren, Guo, Xiaoxin, Askar, Idil, Nahirnyj, Adrian, Flanagan, John G, and Sivak, Jeremy M

Subjects
Neuroglia, Astrocytes, Intermediate Filaments, Animals, Mice, Inbred C57BL, Disease Models, Animal, Gliosis, Imidazoles, Pyridines, p38 Mitogen-Activated Protein Kinases, Tumor Necrosis Factor-alpha, Apoptosis, Male, Withanolides, Retinal Neurons, Polymerization, Corneal Injuries, Mice, Inbred C57BL, Disease Models, Animal, Biochemistry and Cell Biology, Oncology and Carcinogenesis
Abstract

Reactive gliosis is an early pathological feature common to most neurodegenerative diseases, yet its regulation and impact remain poorly understood. Normally astrocytes maintain a critical homeostatic balance. After stress or injury they undergo rapid parainflammatory activation, characterized by hypertrophy, and increased polymerization of type III intermediate filaments (IFs), particularly glial fibrillary acidic protein and vimentin. However, the consequences of IF dynamics in the adult CNS remains unclear, and no pharmacologic tools have been available to target this mechanism in vivo. The mammalian retina is an accessible model to study the regulation of astrocyte stress responses, and their influence on retinal neuronal homeostasis. In particular, our work and others have implicated p38 mitogen-activated protein kinase (MAPK) signaling as a key regulator of glutamate recycling, antioxidant activity and cytokine secretion by astrocytes and related Müller glia, with potent influences on neighboring neurons. Here we report experiments with the small molecule inhibitor, withaferin A (WFA), to specifically block type III IF dynamics in vivo. WFA was administered in a model of metabolic retinal injury induced by kainic acid, and in combination with a recent model of debridement-induced astrocyte reactivity. We show that WFA specifically targets IFs and reduces astrocyte and Müller glial reactivity in vivo. Inhibition of glial IF polymerization blocked p38 MAPK-dependent secretion of TNF-α, resulting in markedly reduced neuronal apoptosis. To our knowledge this is the first study to demonstrate that pharmacologic inhibition of IF dynamics in reactive glia protects neurons in vivo.

Published
2016

41. Mutant RAMP2 causes primary open-angle glaucoma via the CRLR-cAMP axis

Author

Gong, Bo, Zhang, Houbin, Huang, Lulin, Chen, Yuhong, Shi, Yi, Tam, Pancy Oi-Sin, Zhu, Xianjun, Huang, Yi, Lei, Bo, Sundaresan, Periasamy, Li, Xi, Jiang, Linxin, Yang, Jialiang, Lin, Ying, Lu, Fang, Chen, Lijia, Li, Yuanfeng, Leung, Christopher Kai-Shun, Guo, Xiaoxin, Zhang, Shanshan, Huang, Guo, Wu, Yaqi, Zhou, Tongdan, Shuai, Ping, Tham, Clement Chee-Yung, Weisschuh, Nicole, Krishnadas, Subbaiah Ramasamy, Mardin, Christian, Reis, André, Yang, Jiyun, Zhang, Lin, Zhou, Yu, Wang, Ziyan, Qu, Chao, Shaw, Peter X., Pang, Chi-Pui, Sun, Xinghuai, Zhu, Weiquan, Li, Dean Yaw, Pasutto, Francesca, and Yang, Zhenglin

Published
2019
Full Text
View/download PDF

42. High-resolution neuraminidase inhibition profiling of Arnebia euchroma (Royle) I.M. Johnst. based on HR-MS and target isolation: An example study of anti-infectious constituents in traditional Chinese medicine

Author

Huang, Yuheng, primary, Guo, Xiaoxin, additional, Wang, Zhen, additional, Yin, Cong, additional, Chen, Mu, additional, Xie, Jiaming, additional, Li, Ning, additional, Tu, Zhengchao, additional, Li, Juan, additional, Cao, Jiaqing, additional, Jiang, Zhengjin, additional, Huang, Weihuan, additional, and Tian, Haiyan, additional

Published
2023
Full Text
View/download PDF

43. An Improved Edge Detection Method Using Adaptive Threshold

Author

Che, Xiangjiu, Wang, Li, Guo, Xiaoxin, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Weikum, Gerhard, Series editor, Pan, Zhigeng, editor, Cheok, Adrian David, editor, Müller, Wolfgang, editor, and Zhang, Mingmin, editor

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results