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The photosynthetic glycerolipids composition of cyanobacteria is crucial to the structural and physiological aspects of cells. We developed a method of electrospray ionization tandem mass spectrometry coupled with ultra-performance liquid chromatography to qualitatively and quantitatively analyze the lipidomes of the Synechocystis sp. PCC 6803 cells. Shotgun analysis identified photosynthetic glycerolipids, including lipid classes and fatty acyl composition within lipids, and exhibited high efficiency, selectivity, sensitivity and mass accuracy, Under different light intensities, the content of MGDG and PG increased by 34.64% and 68.49% after 2 hours exposition, respectively. The MGDG and PG molecules containing de novo synthesized saturated fatty acids increased more rapidly and drastically than highly unsaturated species. The content of DGDG increased by 26.95% after 12 hours under high light, while the content of SQDG gradually declined. The method established in this study provides a powerful analytical tool for dissecting metabolism and physiological functions of glycerolipids in cyanobacteria. [ABSTRACT FROM AUTHOR]

Objective To investigate the possible role of canonical trasnient receptor potential channels (TRPCs) in calcium overload of neurons after subarachnoid hemorrhage (SAH) and discuss the mechanism of TRPCs involved in delayed neuronal death (DND) after SAH. Methods Rat models of SAH were established by double-blood injection via the citerna magna. SAH intervention group was established by using SKF96365 intracerebroventricular injection. Intracellular calcium concentration was detected by Fura-2AM. The dynamic expressions of TRPCs in the cortex and basilar artery were determined by Western blot. The mRNA levels of TRPCs in the cortex and basilar artery were determined by RT-PCR. The results were compared with those in DMSO control model group, saline control group and normal group. The apoptosis of cortical neurons was detected by TUNEL. One-way ANOVA was used to compare within each group. Results The protein expressions and mRNA levels of TRPC1 in the cortex and the basilar artery continuourly increased after SAH and reached the peak on day 5 while intracellular calcium concentration and TUNEL-positive cell counts also reached the peak. After injection of SKF96365, a TRPCs blocker, the protein expressions and mRNA levels of TRPC1 were inhibited, intracellular calcium concentration and TUNEL-positive cell counts decreased. Meanwhile, the scores of rat neurological functions were improved. The expressions of TRPC3 in the cortex and the basilar artery showed no significant change. Conclusion TRPC1 induced calcium overload and CVS after SAH are the major causes of delayed neuronal death. Suppressing calcium influx induced by TRPC1 could delay neuronal death and improve neurological functions. [ABSTRACT FROM AUTHOR]

Objective: To explore the clinical characteristics of hospitalized patients with hematologic diseases complicated with carbapenem-resistant organisms (CRO) infection and analyze the risk factors of 30-day all-cause mortality., Methods: The clinical data and laboratory test data of 77 hospitalized patients with hematologic diseases complicated with CRO infection in department of hematology of the Third Hospital of Shanxi Medical University from January 2015 to December 2020 were retrospectively analysed, the risk factors of 30-day all-cause mortality after CRO infection were analyzed by multivariate logistic regression., Results: Among the total of 77 patients with hematologic diseases complicated with CRO infection, 29 died and 48 survived within 30 days of infection, with a case fatality rate of 37.66%. A total of 93 strains of CRO were isolated from these patients, of which Acinetobacter baumannii had the highest detection rate (25.81%, 24/93), followed by Pseudomonas aeruginosa (18.28%, 17/93). The lung was the most common site of CRO infection. The detected pathogens were highly resistant to carbapenems, and 64.52% (60/93) of the pathogens were resistant to imipenem with minimum inhibitory concentration (MIC)≥16 μg/ml. The results of the univariate analysis showed that albumin concentration <25 g/L ( P =0.048), serum creatinine concentration≥120 μmol/L ( P =0.023), age-adjusted Charlson comorbidity index (ACCI) ( P =0.037) and primary treatments (supportive treatment, immunosuppressive therapy, chemotherapy, HSCT) ( P =0.048) were significantly associated with 30-day all-cause mortality after infection. The results of multivariate logistic regression analysis showed that when CRO infection confirmed, albumin concentration <25 g/L ( P =0.014, OR =6.171), serum creatinine concentration≥120 μmol/L ( P =0.009, OR =10.867) were independent risk factors for 30-day mortality of patients with hematologic diseases complicated with CRO infection., Conclusion: The mortality rate of CRO-infected patients with hematologic diseases is high. The detected pathogenic bacteria are highly resistant to imipenem. The albumin concentration <25 g/L and the serum creatinine concentration≥ 120 μmol/L at diagnosis of CRO infection were independent risk factors for 30-day mortality of the patients with hematologic diseases.

Objective: To explore the effect of Telbivudine (LDT) Tablet combined with Jianpi Bushen Recipe (JBR) on serum hepatitis B virus (HBV) specific cytotoxic T lymphocyte (CTL) and HBeAg seroconversion in chronic hepatitis B (CHB) patients., Methods: Totally 90 HBeAg-positive and human leukocyte antigen (HLA)-A2 positive CHB patients were randomly assigned to the treatment group and the control group, 45 cases in each group. Patients in the treatment group took LDT Tablet (600 mg, once per day) combined with JBR granule (twice per day), while those in the control group took LDT Tablet alone. The therapeutic course for all was one year. HBV DNA negative conversion rate, HBeAg seroconversion rate, and level of HBV specific CTL were compared after 1 year treatment; liver function, drug resistance mutations, and adverse reactions were also compared between the two groups., Results: After 1 year treatment, HBV DNA negative conversion rate and HBeAg seroconversion rate were 88.89% (40/45) and 40.00% (18/45) in the treatment group, higher than those of the control group [68.89% (31/45) and 20.00% (9/45)], with statistical difference (P < 0.05). Level of HBV specific CTL in the treatment group was 0.78% +/- 0.09% after treatment, higher than that of the control group after 1 year treatment (0.54% +/- 0.11%) and that before treatment (0.36% +/- 0.07%), with statistical difference (P < 0.01). Level of HBV specific CTL in 27 patients with HBeAg seroconversion was 0.81% 0.10%, higher than that of 63 patients without HBeAg seroconversion (0.60% +/- 0.09%), with statistical difference (P < 0.01). ALT returned to normal in 44 cases of the treatment group (97.78%), while it was 42 cases (93.33%) of the control group, with no statistical difference between the two groups (P > 0.05). Total bilirubin (TBil) in the two groups all turned to normal. rtM204I variation occurred in 1 case (2.22%) of the treatment group and 2 cases (4.44%) in the control group. No obvious adverse reaction occurred in the two groups., Conclusion: LDT Tablet combined with JBR could elevate levels of HBV specific CTL and HBeAg seroconversion in CHB patients.

Unlabelled: OBJECTIVE To observe the clinical efficacy by Qingying Huoxue Decoction (QHD) combined ursodeoxycholic acid (UDCA) in treating patients with early and mid-term primary biliary cirrhosis (PBC). METHODS Totally 78 patients were randomly assigned to the treatment group and the control group, 39 in each group. All patients received basic treatment and took UDCA (at the daily dose of 13-15 mg/kg). Patients in the treatment group took QHD, one dose per day. The treatment course for all was 6 weeks. Clinical efficacy, gamma-glutamyl transferase (γ-GGT), alkaline phospatase (ALP), TBIL, alanine aminotransferase (ALT), and aspartate transaminase (AST) were observed before and after treatment. RESULTS Totally 21 (53. 8%) patients obtained complete response in the treatment group, with statistical difference when compared with that of the control group (11 cases, 30. 8%). Levels of GGT, ALP, ALT, AST, and TBIL decreased in the two groups after treatment (P < 0.01). Levels of ALP, GGT, and TBIL were obviously lower in the treatment group than in the control group (P < 0.05)., Conclusions: QHD combined UDCA in treating early and mid-term PBC patients was superior to the effect of using UDCA alone. It also could improve patients' liver function.

Objective: To explore the effect of compound qizhu granule (CQG) on cellular immunity of chronic hepatitis B (CHB) patients., Methods: Totally 103 CHB patients treated with lamivudin (LAM) for 6 months, who had partial virological response (HBeAg positive) were randomly assigned to two groups, 50 in the treatment group and 53 in the control group. All patients took LAM 100 mg (once a day) plus ADV 10 mg (once a day). Patients in the treatment group additionally took CQG, one dose per day. After one-year treatment hepatitis B virus (HBV) DNA negative rates, HBeAg seroconversion, levels of HBV specific cytotoxic T lymphocyte (CTL), non-specific CTL and natural killing (NK) cells were compared between the two groups., Results: After 1-year treatment, HBV DNA negative rate of the treatment group was 88: 0% in 44 cases, slightly higher than that of the control group (41 cases, 77.4%), but with no statistical difference (P >0.05). HBeAg seroconversion of the treatment group was 32.0% in 16 cases, higher than that of the control group (8 cases, 15.1%), with statistical difference (P <0.05). Levels of HBV specific CTL (0.79%±0. 07%), non-specific CTL (19.4%±1.8%) and NK cells (14. 1%± 1.5%) of the treatment group were higher than those of the control group (0.58% ± 0.08%, 17.5% ± 1.7%, and 11.1%±1.5%, respectively; allP <0.01)., Conclusion: Treating CHB patients with partial virological response by ADV plus CQG could improve specific and non-specific cellular immunity, thereby elevating HBeAg seroconversion rate.