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Your search keyword '"Guo, Renpeng"' showing total 25 results
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25 results on '"Guo, Renpeng"'

14. Petunidin-3-O-[rhamnopyranosyl-(trans-p-coumaroyl)]-5-O-(β-D-glucopyranoside), the main anthocyanin from the fruits of Lycium ruthenicummurray, enhances the lifespan of Caenorhabditis elegansby activating DAF-16 and improving the gut microbiota

Author

Zhang, Kangzhi, Zhu, Jiahao, Liu, Peiyun, Guo, Renpeng, Yan, Yamei, Mi, Jia, Lu, Lu, Cao, Youlong, and Zeng, Xiaoxiong

Abstract

Research on anti-aging activity primarily focuses on crude extracts of anthocyanins, while relatively less attention has been given to anthocyanin monomers. This study was focused on exploring the anti-aging properties of the main anthocyanin monomer, petunidin-3-O-[rhamnopyranosyl-(trans-p-coumaroyl)]-5-O-(β-D-glucopyranoside) (P3G), from the fruits of Lycium ruthenicumMurray. The effects of P3G on stress endurance, lifespan, and gut microbiota in Caenorhabditis eleganswere investigated. It was found that P3G significantly extended the mean lifespan by 19.50%, enhanced fitness through increased motility (30.39%) and pharyngeal pumping (18.33%), and reduced lipofuscin accumulation by 43.61% while maintaining fecundity. P3G prolonged lifespan by 20.13% and 18.82% during oxidative and heat stress, correspondingly. This effect was attributed to the activation of stress-related genes and the antioxidant system. The transcriptome results showed the enrichment in the longevity pathway and biosynthesis pathway of nematodes. The prominent role of the DAF-16 pathway was highlighted, as P3G enhanced the expression of daf-16gene and downstream genes (sod-2, sod-3, and mlt-1), promoting nuclear accumulation of DAF-16. Moreover, modifications in the gut microbiota induced by P3G played a role in its anti-aging properties by governing gut microbial composition. Overall, P3G appeared to contribute to promoting the health of nematodes and might help delay their aging to some extent.

Published
2024
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15. Telomere dysfunction impairs epidermal stem cell specification and differentiation by disrupting BMP/pSmad/P63 signaling

Author

Liu, Na, primary, Yin, Yu, additional, Wang, Haiying, additional, Zhou, Zhongcheng, additional, Sheng, Xiaoyan, additional, Fu, Haifeng, additional, Guo, Renpeng, additional, Wang, Hua, additional, Yang, Jiao, additional, Gong, Peng, additional, Ning, Wen, additional, Ju, Zhenyu, additional, Liu, Yifei, additional, and Liu, Lin, additional

Published
2019
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20. Tet Enzymes Regulate Telomere Maintenance and Chromosomal Stability of Mouse ESCs

Author

Yang, Jiao, primary, Guo, Renpeng, additional, Wang, Hua, additional, Ye, Xiaoying, additional, Zhou, Zhongcheng, additional, Dan, Jiameng, additional, Wang, Haiying, additional, Gong, Peng, additional, Deng, Wei, additional, Yin, Yu, additional, Mao, ShiQing, additional, Wang, Lingbo, additional, Ding, Junjun, additional, Li, Jinsong, additional, Keefe, David L., additional, Dawlaty, Meelad M., additional, Wang, Jianlong, additional, Xu, GuoLiang, additional, and Liu, Lin, additional

Published
2016
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22. Telomere Elongation and Naive Pluripotent Stem Cells Achieved from Telomerase Haplo-Insufficient Cells by Somatic Cell Nuclear Transfer

Author

Sung, Li-Ying, primary, Chang, Wei-Fang, additional, Zhang, Qian, additional, Liu, Chia-Chia, additional, Liou, Jun-Yang, additional, Chang, Chia-Chun, additional, Ou-Yang, Huan, additional, Guo, Renpeng, additional, Fu, Haifeng, additional, Cheng, Winston T.K., additional, Ding, Shih-Torng, additional, Chen, Chuan-Mu, additional, Okuka, Maja, additional, Keefe, David L., additional, Chen, Y. Eugene, additional, Liu, Lin, additional, and Xu, Jie, additional

Published
2014
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24. [Prokaryotic expression, protein purification and functional verification of human homotypic fusion and vacuole protein sorting complex subunit].

Author

Chen Y, Guo R, Huang S, Song D, and Liu R

Subjects
Humans, Membrane Fusion, Protein Binding, Protein Transport, Recombinant Fusion Proteins, Autophagy, Vacuoles
Abstract

Homotypic fusion and vacuole protein sorting(HOPS) is a protein complex consisting of VPS11, VPS16, VPS18, VPS33, VPS39, VPS41 and regulates membrane transport in vivo through membrane fusion mechanisms. The evidence suggests that HOPS complex as a fusion factor, facilitates autophagosome-lysosome fusion. To determine whether the HOPS complex directly interacts with the autophagic SNARE protein STX17 in vitro, the coding sequence of the six genes were amplified from the existing plasmids by PCR, and then ligated to the prokaryotic expression vector pGEX 4T-1-GST or pET-His-NusA. After identification through colony PCR and DNA sequencing, 6 recombinant plasmids were constructed and transferred into Escherichia coli BL21 (DE3). The recombinant proteins were purified by glutathione sepharose 4B and nickel column. We used the tobacco etch virus protease to cut off the GST-tag or His-NusA-tag, to obtain HA-VPS11 protein of about 105 kDa, Flag-VPS16 protein of about 97 kDa, HA-VPS18 protein of about 108 kDa, Flag-VPS33 protein of about 70 kDa, HA-VPS39 protein of about 97 kDa, and Flag-VPS41 protein of about 98 kDa. The function of the purified proteins was verified by in vitro glutathione S-transferases pull-down assay, confirming that autophagic SNARE protein STX17 interacted directly with HOPS components. Our findings provide experimental basis to further study the function and mechanism of HOPS complex in the process of autophagosome-lysosome fusion.

Published
2020
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25. 2-Amino-3,8-dimethylimidazo[4,5- f ]quinoxaline Alters Autophagosome Maturation, Cellular Lipidomic Profiles, and Expression of Core Pluripotent Factors.

Author

Song D, Guo R, Huang H, Zheng P, Huang H, Oyang Q, Xiao X, Wang B, Rong J, and Liu R

Subjects
Animals, Autophagosomes metabolism, Cell Line, Embryonic Stem Cells drug effects, Embryonic Stem Cells metabolism, Humans, Lipid Metabolism drug effects, Mice, Microtubule-Associated Proteins genetics, Microtubule-Associated Proteins metabolism, Transcription Factors genetics, Autophagosomes drug effects, Lipids chemistry, Quinoxalines pharmacology, Transcription Factors metabolism
Abstract

2-Amino-3,8-dimethylimidazo[4,5- f ]quinoxaline (MeIQx), one of the most abundant heterocyclic aromatic amines (HAAs) found in the human diet, is primarily produced during high-temperature meat or fish cooking. While MeIQx has been investigated as a potential carcinogen, the cytotoxicity and related molecular mechanisms remain unclear. Here, we demonstrate that autophagosome maturation is blocked by MeIQx. Mechanistically, MeIQx inhibits acidification of lysosomes rather than prevents autophagosome-lysosome fusion. Moreover, cellular lipid profiles are altered by MeIQx treatment. Notably, many phospholipids and sphingolipids are significantly upregulated after exposure to MeIQx. Furthermore, MeIQx decreases expression of pluripotency-associated proteins in mouse embryonic stem cells (ESCs). Together, MeIQx blocks autophagosome maturation through inhibiting acidification of lysosomes, alters lipid metabolism, and decreases expression of pluripotent factors. Our studies provide more cytotoxic evidence and elucidate related mechanisms on the risk of HAA exposure and are expected to promote supervision of food safety and human health.

Published
2019
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