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Ubiquitination signaling is the main pathway of protein degradation in eukaryotic cells. Ubiquitin-proteasome system degrades the ubiquitinated cytoplasmic proteins and lysosome pathway mainly degrades the ubiquitinated membrane proteins. Previous studies have shown that ubiquitination signaling plays a critical role in fatty acids synthesis. In the process of fatty acids import, disruption of ubiquitination could prevent the degradation of fatty acid transport proteins, thereby promoting fatty acids import and milk fat synthesis in bovine primary mammary epithelial cells. In this review, we summarize the signal transduction and regulation mechanism of ubiquitination signaling in milk fat synthesis, which may provide references and new ideas for future research on milk fat traits in dairy cows.

In a previous genome-wide association study on milk production traits in Chinese Holstein population, we discovered VPS28 gene was highly expressed in mammary gland tissue. Further, a -58C>T mutantion at the 5°-UTR of the gene was significantly associated with milk fat content traits. However, its regulation on milk fat content is still uncertain. In this study, we explored the effect of this -58C>T mutation on VPS28, and found that it could significantly reduce promoter activity of VPS28 gene. To identify the potential function of this SNP, we performed RNAi experiments to knockdown VPS28 gene in BMECs and examined the general effects of VPS28 knockdown on BMECs. The results showed that VPS28 knockdown could decrease the mRNA expression of genes in ubiquitination-lysosome and ubiquitination- proteasome pathways, increase the mRNA expression of genes in milk fat synthesis pathway and promote triglyceride synthesis in BMECs. Our data indicate that VPS28 gene could regulate milk fat synthesis pathway; and promote triglyceride synthesis in BMECs. Our data indicate that VPS28 gene could regulate milk fat synthesis through modulating the ubiquitination-lysosome and ubiquitination-proteasome systems. Our results demonstrate the molecular mechanism of VPS28 on regulation of milk synthesis in dairy cattle through the ubiquitination signaling pathway, thereby supporting a relationship between milk fat synthesis and ubiquitination and laying the molecular foundation in breeding of dairy cattle in the future.

Food selection by folivorous primates is thought to relate to macronutrients, micronutrients and plant secondary metabolites. However, few studies explain their effects on food choices. This study was designed to clarify the effect of phytochemical components on Rhinopithecus bieti food choice by analyzing the chemical composition of food samples collected from March to May in 2010 and 2011 at Mt. Lasha in northwest Yunnan, China. Compared with non-foods, there was more phosphorous and crude protein, less total sugar and a lower ratio of calcium to phosphorus in selected foods. However, no differences were found in crude fat, crude ash, calcium and tannin content between foods and non-foods. Phytochemical constituents may influence food choices; the monkeys preferred foods with high phosphorus and low Ca/P, low calcium, low sugar and low tannins. Rhinopithecus bieti foraged high quality foods such as buds and young leaves to meet their nutritional needs after a long winter. Therefore, if tannin content in food did not exceed the enduring threshold of R. bieti, the nutrient intake was prioritized by phosphorus and calcium regulation when the need for macronutrients dominated by protein was satisfied.