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The occurrence of pharmaceuticals in the environment can have undesirable effects on nontarget animals, including fish. The present experiment assessed the effects of subchronic exposure to waterborne acetaminophen (N-acetyl-p-aminophenol) (APAP) on selected behavioral aspects (physical avoidance response, ventilation rate, and food detection rate) and erythrocyte nuclear abnormality (ENA) in juvenile Oreochromis niloticus . Two groups of fish were exposed to APAP dissolved in aged municipal water (T 1 : 2 mg/L and T 2 : 10 mg/L) for 8 weeks in three replicates ( n  = 7 fish per tank), alongside a control group (C) without APAP. APAP-exposed fish spent significantly ( p  < .05) longer time to detect food (T 1 : 32.6 ± 4.55 s and T 2 : 39.6 ± 4.66 s) compared to the control group (19.9 ± 2.46 s). Both APAP-exposed groups exhibited attenuated physical avoidance responses (76.7%, 68.7%, and 87.3% in T 1 , T 2 , and C, respectively) and a lower mean ventilation rate compared to the control group (194.5 ± 15.5, 179.1 ± 11.6, and 233.2 ± 19.0 per min in T 1 , T 2 , and C, respectively). The frequency occurrence of ENA types such as bi-nucleated, notched nuclei, lobed nuclei, and blebbed nuclei (except micronuclei) was significantly higher ( p  < .05) in APAP-exposed groups compared to the control, with more pronounced effects in the T 2 group. The study concludes that APAP exposure prompts significant alterations in behavior and erythrocyte nuclear morphology, emphasizing the value of monitoring and regulating the entry of pharmaceuticals, including APAP, into aquatic environments to prevent unintended effects on non-target organisms like Oreochromis niloticus .

The present study evaluated the cadmium (Cd) levels and temporal variation of Cd in dark muscle, white muscle, and liver of juvenile Thunnus albacares. 72 individuals (Standard length: 50-67 cm; weight: 0.8-2.5 kg) were collected from Indian Oceanic water around Sri Lanka during the period between April 2021 to May 2022. Total Cd levels were analyzed using an Inductively Coupled Plasma Mass Spectrophotometer. The mean Cd levels (mean ± SD mg kg -1 dry weight) in different tissues varied with significantly higher levels in the liver (13.62 ± 0.98, p < 0.05), compared to dark muscle (0.52 ± 0.05), and white muscle (0.42 ± 0.04). Cd levels in liver tissues were positively correlated (p < 0.05) with the fish weight. The Cd levels reported in dark muscles, white muscles, and liver tissues were significantly higher (p < 0.05) during 2nd inter-monsoon than in the other monsoonal regimes and exceeded the maximum permissible level (0.1 mg kg -1 wet weight) set by the European Union (EU). However, the measured Cd levels in white and dark muscles were below the maximum permissible level (0.2 mg kg -1 wet weight) set by FAO/WHO. The Cd levels in all the liver tissues were above the levels set by the EU and FAO/WHO. Accordingly, people should avoid the consumption of liver tissues of T. albacares from the Indian Ocean. A human with a body weight of 60 kg can consume white muscles up to 4.667 kg per week without exceeding the Provisional Tolerable Weekly Intake., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Chronic kidney disease of unknown etiology was investigated for metal relations in an endemic area by a cross-sectional study with CKD stages G1, G2, G3a, G3b, G4, G5 (ESRD), and endemic and nonendemic controls (EC and NEC) as groups. Subjects with the medical diagnosis were classified into groups by eGFR (SCr, CKD-EPI) and UACR of the study. It determined 24 metals/metalloids in plasma (ICPMS) and metallothionein (MT) mRNA in blood (RT-PCR). MT1A at G3b and MT2A throughout G2−G5 showed increased transcription compared to NEC (ANOVA, p < 0.01). Both MT1A and MT2A remained metal-responsive as associations emerged between MT2A and human MT inducer Cr (in EC: r = 0.54, p < 0.05, n = 14), and between MT1A and MT2A (in EC pooled with G1−G5: r = 0.58, p < 0.001, n = 110). Human MT (hMT)-inducers, namely Zn, Cu, As, Pb, and Ni; Σ hMT-inducers; 14 more non-inducer metals; and Σ MT-binding metals remained higher (p < 0.05) in EC as compared to NEC. Declining eGFR or CKD progression increased the burden of Be, Mg, Al, V, Co, Ni, Rb, Cs, Ba, Mn, Zn, Sr, Σ hMT-inducers, and Σ MT-binding metals in plasma, suggesting an MT role in the disease. MT1A/2A mRNA followed UACR (PCA, Dendrogram: similarity, 57.7%). The study provides evidence that proteinuric chronic renal failure may increase plasma metal levels where blood MT2A could be a marker.

CKDu needs to be characterized in fundamental areas to improve etiological understanding and disease management. In a cross-sectional study, blood cell profile and plasma inflammatory cytokines were followed by automated analysis and sandwich ELISA, respectively. Disease development stages and proteinuria were ascertained by eGFR and UACR. Comparison among control and stages (ANOVA/Dunnett's MRT) revealed time-specific changes ( p < 0.05), including decreased erythrocytes (G5) and hematocrit (G5), and increased MCHC (G3b, G4), MCV (G5), and MCH (G5). CKDu decreased ( p < 0.05) lymphocytes (G3b, G4, G5), monocytes (G3b), MPV (G3b, G4, G5), and plateletcrit (G3b, G4), and increased basophils (G3a, G3b, G4), N/L (G4) and PLR (G4-G5). MCHC and aforesaid leukocyte variables were in correlation (rho > ±0.03, p < 0.05, Pearson's test) with disease development. MCP-1 and IL-6 spiked ( p > 0.05) at G3b. Multivariate analyses confirmed that MCP-1, lymphocytes, and BMI were related to renal dysfunction, pointing to inflammation, compromised immunity, and muscle wasting as CKDu effects. Nonproteinuric CKDu was prevalent (23.2-35.6% of total CKDu) with ( p < 0.05) elevated basophils (G3a), N/L (G4), and depleted lymphocytes (G4). In both forms, G1-G2 were unaffected, and the earliest change was G3a basophils. Results suggest that MCP-1, lymphocyte count, N/L, and PLR may verify the stage and predict impending ESRD in advance proteinuric CKDu.