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2. Features of cryptococcosis among 652 HIV-seronegative individuals in France: a cross-sectional observational study (2005-2020)

Author

Brieu, N., Durand, C., Bertei, D., Bouchara, J.P., Pihet, M., Bland, S., Bru, J.P., Pulik, M., Le Turdu, F., Lefrand C, H., Ferrand, M., Larrouy, M., Millon, L., Delhaes, L., Imbert, S., Accoceberry, I., Bachelier, M.N., Nevez, G., Quinio, D., Le Coustumier, A., Carmagnol, F., Rivière, B., Boex, P., Podac, B., Moniot, M., Nourrisson, C., Augereau, O., Emond, J.P., Belkacem-Belkaki, G., Bacri, J.L., Berthelot, G., Dalle, F., Vallee, E., Bizet, J., Noussair, L., Herrmann, J.L., Maubon, D., Brocard, C., Guiffault, P., Layet, A., Morel, A., Angoulvant, A., Penn, P., Gigandon, A., Sendid, B., Cornu, M., Darde, M.L., Jaccard, A., Bouteille, B., Azjenberg, D., Prades, N., Bienvenu, A.L., Benoit-Cattin, T., Fiacre, A., Levy, S., Pitsch, A., Kiefer, M.H., Debourgogne, A., Moquet, O., Colot, J., Courtellemont, L., Poisson, D., Laurens, V., Kauffmann-Lacroix, C., Martres, P., Gargala, G., Godineau, N., Picot, S., Chassagne, C., Djibo, N., Devallière, R., Sabou, M., Camin-Ravenne, A.M., Bissuel, F., Janvier, F., Aubert, X., Chadapaud, S., Delbeck, X., Lafeuillade, A., Raoult, X., Baclet, V., Coignard, C., Mouton, Y., Ravaux, I., Eloy, C., Fur, A., Rezzouk, L., Mazards, E., Eloy, O., Chachaty, E., Mihaila, L., Dellion, S., Patey, O., Thouvenot, A., Limousin, L., Paugam, A., Desplaces, N., Raguin, G., Sitterlé, E., Blaize, M., Gits-Muselli, M., Hennequin, C., Poirot, J.L., Bretagne, S., Lacroix, Claire, Hamane, Samia, Paccoud, Olivier, Desnos-Ollivier, Marie, Persat, Florence, Demar, Magalie, Boukris-Sitbon, Karine, Bellanger, Anne-Pauline, Bonhomme, Julie, Bonnal, Christine, Botterel, Françoise, Bougnoux, Marie-Elisabeth, Brun, Sophie, Cassaing, Sophie, Cateau, Estelle, Chouaki, Taieb, Cornet, Muriel, Dannaoui, Eric, Desbois-Nogard, Nicole, Durieux, Marie-Fleur, Favennec, Loïc, Fekkar, Arnaud, Gabriel, Frederic, Gangneux, Jean-Pierre, Guitard, Juliette, Hasseine, Lilia, Huguenin, Antoine, Le Gal, Solène, Letscher-Bru, Valérie, Mahinc, Caroline, Morio, Florent, Nicolas, Muriel, Poirier, Philippe, Ranque, Stéphane, Roosen, Gabrielle, Rouges, Célia, Roux, Anne-Laure, Sasso, Milène, Alanio, Alexandre, Lortholary, Olivier, and Lanternier, Fanny

Published
2024
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3. MixInYeast: A Multicenter Study on Mixed Yeast Infections

Author

Medina, Narda, Soto-Debrán, Juan Carlos, Seidel, Danila, Akyar, Isin, Badali, Hamid, Barac, Aleksandra, Bretagne, Stéphane, Cag, Yasemin, Cassagne, Carole, Castro, Carmen, Chakrabarti, Arunaloke, Dannaoui, Eric, Cardozo, Celia, Garcia-Rodriguez, Julio, Guitard, Juliette, Hamal, Petr, Hoenigl, Martin, Jagielski, Tomasz, Khodavaisy, Sadegh, Lo Cascio, Giuliana, Martínez-Rubio, María Carmen, Meletiadis, Joseph, Muñoz, Patricia, Ochman, Elżbieta, Peláez, Teresa, Perez-Ayala Balzola, Ana, Prattes, Juergen, Roilides, Emmanuel, Ruíz-Pérez de Pipaón, Maite, Stauf, Raphael, Steinmann, Jörg, Suárez-Barrenechea, Ana Isabel, Tejero, Rocío, Trovato, Laura, Viñuela, Lourdes, Wongsuk, Thanwa, Żak, Iwona, Zarrinfar, Hossein, Lass-Flörl, Cornelia, Arikan-Akdagli, Sevtap, and Alastruey-Izquierdo, Ana

Subjects
Vaccine Related, Biodefense, Prevention, Infectious Diseases, Antimicrobial Resistance, Infection, yeast, chrome agar, invasive candidiasis, Candida, mix infections, polymicrobial infections
Abstract

Invasive candidiasis remains one of the most prevalent systemic mycoses, and several studies have documented the presence of mixed yeast (MY) infections. Here, we describe the epidemiology, clinical, and microbiological characteristics of MY infections causing invasive candidiasis in a multicenter prospective study. Thirty-four centers from 14 countries participated. Samples were collected in each center between April to September 2018, and they were sent to a reference center to confirm identification by sequencing methods and to perform antifungal susceptibility testing, according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST). A total of 6895 yeast cultures were identified and MY occurred in 150 cases (2.2%). Europe accounted for the highest number of centers, with an overall MY rate of 4.2% (118 out of 2840 yeast cultures). Of 122 MY cases, the most frequent combinations were Candida albicans/C. glabrata (42, 34.4%), C. albicans/C. parapsilosis (17, 14%), and C. glabrata/C. tropicalis (8, 6.5%). All Candida isolates were susceptible to amphotericin B, 6.4% were fluconazole-resistant, and two isolates (1.6%) were echinocandin-resistant. Accurate identification of the species involved in MY infections is essential to guide treatment decisions.

Published
2021

4. MixInYeast: A Multicenter Study on Mixed Yeast Infections.

Author

Medina, Narda, Soto-Debrán, Juan Carlos, Seidel, Danila, Akyar, Isin, Badali, Hamid, Barac, Aleksandra, Bretagne, Stéphane, Cag, Yasemin, Cassagne, Carole, Castro, Carmen, Chakrabarti, Arunaloke, Dannaoui, Eric, Cardozo, Celia, Garcia-Rodriguez, Julio, Guitard, Juliette, Hamal, Petr, Hoenigl, Martin, Jagielski, Tomasz, Khodavaisy, Sadegh, Lo Cascio, Giuliana, Martínez-Rubio, María Carmen, Meletiadis, Joseph, Muñoz, Patricia, Ochman, Elżbieta, Peláez, Teresa, Perez-Ayala Balzola, Ana, Prattes, Juergen, Roilides, Emmanuel, Ruíz-Pérez de Pipaón, Maite, Stauf, Raphael, Steinmann, Jörg, Suárez-Barrenechea, Ana Isabel, Tejero, Rocío, Trovato, Laura, Viñuela, Lourdes, Wongsuk, Thanwa, Żak, Iwona, Zarrinfar, Hossein, Lass-Flörl, Cornelia, Arikan-Akdagli, Sevtap, and Alastruey-Izquierdo, Ana

Subjects
Candida, chrome agar, invasive candidiasis, mix infections, polymicrobial infections, yeast
Abstract

Invasive candidiasis remains one of the most prevalent systemic mycoses, and several studies have documented the presence of mixed yeast (MY) infections. Here, we describe the epidemiology, clinical, and microbiological characteristics of MY infections causing invasive candidiasis in a multicenter prospective study. Thirty-four centers from 14 countries participated. Samples were collected in each center between April to September 2018, and they were sent to a reference center to confirm identification by sequencing methods and to perform antifungal susceptibility testing, according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST). A total of 6895 yeast cultures were identified and MY occurred in 150 cases (2.2%). Europe accounted for the highest number of centers, with an overall MY rate of 4.2% (118 out of 2840 yeast cultures). Of 122 MY cases, the most frequent combinations were Candida albicans/C. glabrata (42, 34.4%), C. albicans/C. parapsilosis (17, 14%), and C. glabrata/C. tropicalis (8, 6.5%). All Candida isolates were susceptible to amphotericin B, 6.4% were fluconazole-resistant, and two isolates (1.6%) were echinocandin-resistant. Accurate identification of the species involved in MY infections is essential to guide treatment decisions.

Published
2020

7. Fungal infections in mechanically ventilated patients with COVID-19 during the first wave: the French multicentre MYCOVID study

Author

Gangneux, Jean-Pierre, Dannaoui, Eric, Fekkar, Arnaud, Luyt, Charles-Edouard, Botterel, Françoise, De Prost, Nicolas, Tadié, Jean-Marc, Reizine, Florian, Houzé, Sandrine, Timsit, Jean-François, Iriart, Xavier, Riu-Poulenc, Béatrice, Sendid, Boualem, Nseir, Saad, Persat, Florence, Wallet, Florent, Le Pape, Patrice, Canet, Emmanuel, Novara, Ana, Manai, Melek, Cateau, Estelle, Thille, Arnaud W, Brun, Sophie, Cohen, Yves, Alanio, Alexandre, Mégarbane, Bruno, Cornet, Muriel, Terzi, Nicolas, Lamhaut, Lionel, Sabourin, Estelle, Desoubeaux, Guillaume, Ehrmann, Stephan, Hennequin, Christophe, Voiriot, Guillaume, Nevez, Gilles, Aubron, Cécile, Letscher-Bru, Valérie, Meziani, Ferhat, Blaize, Marion, Mayaux, Julien, Monsel, Antoine, Boquel, Frédérique, Robert-Gangneux, Florence, Le Tulzo, Yves, Seguin, Philippe, Guegan, Hélène, Autier, Brice, Lesouhaitier, Matthieu, Pelletier, Romain, Belaz, Sorya, Bonnal, Christine, Berry, Antoine, Leroy, Jordan, François, Nadine, Richard, Jean-Christophe, Paulus, Sylvie, Argaud, Laurent, Dupont, Damien, Menotti, Jean, Morio, Florent, Soulié, Marie, Schwebel, Carole, Garnaud, Cécile, Guitard, Juliette, Le Gal, Solène, Quinio, Dorothée, Morcet, Jeff, Laviolle, Bruno, Zahar, Jean-Ralph, and Bougnoux, Marie-Elisabeth

Published
2022
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9. Invasive Aspergillosis with impaired neutrophil responses against Aspergillus fumigatus in patients treated with Acalabrutinib—findings from three cases

Author

Blaize, Marion, primary, Thizy, Guillaume, additional, Boissonnas, Alexandre, additional, Portalier, Anaïs, additional, Lanternier, Fanny, additional, de La Porte des Vaux, Clémentine, additional, Suarez, Felipe, additional, Bougnoux, Marie-Elisabeth, additional, Guitard, Juliette, additional, Jabet, Arnaud, additional, Stocker, Nicolas, additional, Aoudjhane, Abdelmalek, additional, Roos-Weil, Damien, additional, and Fekkar, Arnaud, additional

Published
2024
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10. Multicenter evaluation of the Toxoplasma gondii Real-TM (Sacace) kit performance for the molecular diagnosis of toxoplasmosis

Author

Guitard, Juliette, primary, Brenier-Pinchart, Marie-Pierre, additional, Varlet-Marie, Emmanuelle, additional, Dalle, Frédéric, additional, Rouges, Celia, additional, Argy, Nicolas, additional, Bonhomme, Julie, additional, Capitaine, Agathe, additional, Guégan, Hélène, additional, Lavergne, Rose-Anne, additional, Dardé, Marie-Laure, additional, Pelloux, Hervé, additional, Robert-Gangneux, Florence, additional, Yera, Hélène, additional, and Sterkers, Yvon, additional

Published
2024
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11. Features of cryptococcosis among 652 HIV-seronegative individuals in France: a cross-sectional observational study (2005-2020)

Author

Paccoud, Olivier, primary, Desnos-Ollivier, Marie, additional, Persat, Florence, additional, Demar, Magalie, additional, Boukris-Sitbon, Karine, additional, Bellanger, Anne-Pauline, additional, Bonhomme, Julie, additional, Bonnal, Christine, additional, Botterel, Françoise, additional, Bougnoux, Marie-Elisabeth, additional, Brun, Sophie, additional, Cassaing, Sophie, additional, Cateau, Estelle, additional, Chouaki, Taieb, additional, Cornet, Muriel, additional, Dannaoui, Eric, additional, Desbois-Nogard, Nicole, additional, Durieux, Marie-Fleur, additional, Favennec, Loïc, additional, Fekkar, Arnaud, additional, Gabriel, Frederic, additional, Gangneux, Jean-Pierre, additional, Guitard, Juliette, additional, Hasseine, Lilia, additional, Huguenin, Antoine, additional, Le Gal, Solène, additional, Letscher-Bru, Valérie, additional, Mahinc, Caroline, additional, Morio, Florent, additional, Nicolas, Muriel, additional, Poirier, Philippe, additional, Ranque, Stéphane, additional, Roosen, Gabrielle, additional, Rouges, Célia, additional, Roux, Anne-Laure, additional, Sasso, Milène, additional, Alanio, Alexandre, additional, Lortholary, Olivier, additional, Lanternier, Fanny, additional, Brieu, N., additional, Durand, C., additional, Bertei, D., additional, Bouchara, J.P., additional, Pihet, M., additional, Bland, S., additional, Bru (Annecy), J.P., additional, Pulik, M., additional, Le Turdu, F., additional, Lefrand, C, H., additional, Ferrand, M., additional, Larrouy, M., additional, Millon, L., additional, Delhaes, L., additional, Imbert, S., additional, Accoceberry, I., additional, Bachelier, M.N., additional, Nevez, G., additional, Quinio, D., additional, Le Coustumier, A., additional, Carmagnol, F., additional, Rivière, B., additional, Boex, P., additional, Podac, B., additional, Moniot, M., additional, Nourrisson, C., additional, Augereau, O., additional, Emond, J.P., additional, Belkacem-Belkaki, G., additional, Bacri, J.L., additional, Berthelot, G., additional, Dalle, F., additional, Vallee, E., additional, Bizet, J., additional, Noussair, L., additional, Herrmann, J.L., additional, Maubon, D., additional, Brocard, C., additional, Guiffault, P., additional, Layet, A., additional, Morel, A., additional, Angoulvant, A., additional, Penn, P., additional, Gigandon, A., additional, Sendid, B., additional, Cornu, M., additional, Darde, M.L., additional, Jaccard, A., additional, Bouteille, B., additional, Azjenberg, D., additional, Prades, N., additional, Bienvenu, A.L., additional, Benoit-Cattin, T., additional, Fiacre, A., additional, Levy, S., additional, Pitsch, A., additional, Kiefer, M.H., additional, Debourgogne, A., additional, Moquet, O., additional, Colot, J., additional, Courtellemont, L., additional, Poisson, D., additional, Laurens, V., additional, Kauffmann-Lacroix, C., additional, Martres, P., additional, Gargala, G., additional, Godineau, N., additional, Picot, S., additional, Chassagne, C., additional, Djibo, N., additional, Devallière, R., additional, Sabou, M., additional, Camin-Ravenne, A.M., additional, Bissuel, F., additional, Janvier, F., additional, Aubert, X., additional, Chadapaud, S., additional, Delbeck, X., additional, Lafeuillade, A., additional, Raoult, X., additional, Baclet, V., additional, Coignard, C., additional, Mouton, Y., additional, Ravaux, I., additional, Eloy, C., additional, Fur, A., additional, Rezzouk, L., additional, Mazards, E., additional, Eloy, O., additional, Chachaty, E., additional, Mihaila, L., additional, Dellion, S., additional, Patey, O., additional, Thouvenot, A., additional, Limousin, L., additional, Paugam, A., additional, Desplaces, N., additional, Raguin, G., additional, Sitterlé, E., additional, Blaize, M., additional, Gits-Muselli, M., additional, Hennequin, C., additional, Poirot, J.L., additional, Bretagne, S., additional, Lacroix, Claire, additional, and Hamane, Samia, additional

Published
2024
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14. Molecular epidemiology of Candida africana isolates collected from vagina swabs in French Guiana.

Author

Bigot, Jeanne, Kalboussi, Yasmine, Bonkoto Nkoy, Yannick, Benmostefa, Alexis, Vellaissamy, Sandra, Benzerara, Laurent, Sainte-Rose, Vincent, Blanchet, Denis, Demar, Magalie, Guitard, Juliette, and Hennequin, Christophe

Abstract

Previous molecular studies have shown that Candida africana corresponds to the clade 13 of Candia albicans. It has been mostly involved in vulvovaginal candidiasis worldwide but few data exist in South America. The aim of our study was to investigate the prevalence of C. africana in women living in French Guiana. For this, we first set up a fluorescent-intercalating-dye-real time Polymerase Chain Reaction (PCR) targeting the hyphal wall protein 1 gene. The test was applied to 212 C. albicans isolates collected from May to August 2019 from vaginal swabs, allowing the identification of six women harboring C. africana (eight isolates). The in vitro susceptibility of these eight isolates to six antifungal drugs was also evaluated. No demographics or clinical-specific features could be demonstrated. Genetic diversity of those isolates was analyzed through multilocus sequence typing and showed that diploid sequence type 182 was predominant (n  = 6) and allowed the report of a new diploid sequence type. [ABSTRACT FROM AUTHOR]

Published
2024
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15. ß-D-Glucan Assay in the Cerebrospinal Fluid for the Diagnosis of Non-cryptococcal Fungal Infection of the Central Nervous System: A Retrospective Multicentric Analysis and a Comprehensive Review of the Literature

Author

Bigot, Jeanne, primary, Leroy, Jordan, additional, Chouaki, Taieb, additional, Cholley, Laurence, additional, Bigé, Naïke, additional, Tabone, Marie-Dominique, additional, Brissot, Eolia, additional, Thorez, Sophie, additional, Maizel, Julien, additional, Dupont, Hervé, additional, Sendid, Boualem, additional, Hennequin, Christophe, additional, and Guitard, Juliette, additional

Published
2023
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18. Detection of circulating DNA for the diagnosis of invasive fusariosis: retrospective analysis of 15 proven cases

Author

Dellière, Sarah, primary, Guitard, Juliette, additional, Sabou, Marcela, additional, Angebault, Cécile, additional, Moniot, Maxime, additional, Cornu, Marjorie, additional, Hamane, Samia, additional, Bougnoux, Marie-Elisabeth, additional, Imbert, Sébastien, additional, Pasquier, Grégoire, additional, Botterel, Françoise, additional, Garcia-Hermoso, Dea, additional, and Alanio, Alexandre, additional

Published
2022
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19. Molecular Diagnosis of Toxoplasmosis : Multicenter Evaluation of the Toxoplasma RealCycler Universal PCR Assay on 168 Characterized Human Samples

Author

Brenier-Pinchart, Marie-Pierre, Filisetti, Denis, Cassaing, Sophie, Varlet-Marie, Emmanuelle, Robert-Gangneux, Florence, Delhaes, Laurence, Guitard, Juliette, Yéra, Hélène, Bastien, Patrick, Pelloux, Hervé, Sterkers, Yvon, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), CHU Montpellier, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut de Parasitologie et de Pathologie Tropicale (IPPTS), Université de Strasbourg (UNISTRA), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Centre Hospitalier Universitaire [Rennes], Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), CHU Bordeaux [Bordeaux], CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), and Cognata, Bérangère

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], Humans, DNA, Protozoan, Reference Standards, Real-Time Polymerase Chain Reaction, Nucleic Acid Amplification Techniques, Sensitivity and Specificity, Toxoplasma, Toxoplasmosis
Abstract

International audience; Real-time PCR plays a crucial role in the diagnosis of toxoplasmosis. In this multicenter study, the Toxoplasma RealCycler Universal assay was assessed for the diagnosis of toxoplasmosis by eight reference laboratories. DNAs from diverse clinical samples were included: 141 characterized samples from patients with different clinical forms of proven toxoplasmosis and 27 from patients without toxoplasmosis were tested in duplicate with the commercial assay. Final diagnosis was affirmed by each center by analysis of clinical settings and biological follow-up. Calibrated Toxoplasma gondii standards and 11 external quality control samples were also included. Discrepant results observed after the first run of commercial PCR were controlled by both reference and commercial PCR assays. Using the commercial assay, the detection threshold varied from 0.01 to 1 tachyzoites/mL, depending on the center. The relationship between crossing point and DNA concentration was linear over 4 log units (r2 > 0.99), and PCR efficiencies were satisfactory (89% to 104%). The results of the 11 external quality control samples were concordant after one retesting, but those for 3 clinical samples remained discrepant. Sensitivity and specificity were calculated at 97.8% (97.8% to 100%) and 100% (87.2% to 100%), respectively. Provided that PCRs are performed at least in duplicate to detect low parasitic loads, Toxoplasma RealCycler Universal PCR showed suitable performances to diagnose the different forms of toxoplasmosis.

Published
2022
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20. Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study

Author

Monpierre, Lorra, primary, Aït-Ammar, Nawel, additional, Valsecchi, Isabel, additional, Normand, Anne-Cécile, additional, Guitard, Juliette, additional, Riat, Arnaud, additional, Huguenin, Antoine, additional, Bonnal, Christine, additional, Sendid, Boualem, additional, Hasseine, Lilia, additional, Raberin, Hélène, additional, Dehais, Marion, additional, Ranque, Stéphane, additional, Hennequin, Christophe, additional, Piarroux, Renaud, additional, Dannaoui, Eric, additional, and Botterel, Françoise, additional

Published
2022
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22. Coronavirus Disease 2019-Associated Mucormycosis in France: A Rare but Deadly Complication

Author

Danion, François, Letscher-Bru, Valérie, Guitard, Juliette, Sitbon, Karine, Dellière, Sarah, Angoulvant, Adela, Desoubeaux, Guillaume, Botterel, Francoise, Bellanger, Anne-Pauline, Gargala, Gilles, Uhel, Fabrice, Bougnoux, Marie-Elisabeth, Gerber, Victor, Michel, Justin, Cornu, Marjorie, Bretagne, Stéphane, Lanternier, Fanny, Merdji, Hamid, Delabranche, Xavier, Parrot, Antoine, Voiriot, Guillaume, Urbina, Tomas, Mebazaa, Alexandre, Chousterman, Benjamin, el Kalioubie, Ahmed, Six, Sophie, Coulon, Pauline, Sendid, Boualem, Anguel, Nadia, Damoisel, Charles, Mussini, Charlotte, Villate, Alban, Navellou, Jean-Christophe, Girault, Christophe, Cassagne, Carole, Augereau, Olivier, Dromer, Francoise, Garcia-Hermoso, Dea, Lortholary, Olivier, Alanio, Alexandre, Center of Experimental and Molecular Medicine, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg (UNISTRA), Immuno-Rhumatologie Moléculaire, Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Fédération Hospitalo-Universitaire (OMICARE), Centre de Recherche d’Immunologie et d’Hématologie [Strasbourg], CHU Strasbourg, Dynamique des interactions hôte pathogène (DIHP), Mucoviscidose: physiopathologie et phénogénomique [CRSA], Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Mycologie moléculaire - Molecular Mycology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals (CNRMA), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), COVID-Mucor study group, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Service de parasitologie, mycologie, médecine tropicale [CHRU Tours], Dynamic Microbiology - EA 7380 (DYNAMIC), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), CHU Henri Mondor [Créteil], Service de parasitologie et mycologie [CHRU de Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Laboratoire de Biologie Médicale de Référence Pneumopathie d'hypersensibilité, Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), CHU Necker - Enfants Malades [AP-HP], Aix Marseille Université (AMU), CHU Lille, Service de Médecine Intensive et Réanimation [Strasbourg], Les Hôpitaux Universitaires de Strasbourg (HUS), CHU Tenon [AP-HP], Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), COVID-Mucor St Group Hamid Merdji (Médecine Intensive et Réanimation, Hôpitaux Universitaires de Strasbourg, Strasbourg, France), Xavier Delabranche (Réanimation Chirugicale, Hôpitaux Universitaires de Strasbourg, Strasbourg, France), Antoine Parrot (Service de Pneumologie, Hôpital Tenon, AP-HP, Paris, France), Guillaume Voiriot (Service de Médecine Intensive et Réanimation, Hôpital Tenon, AP-HP, Paris, France), Tomas Urbina (Service de Réanimation Médicale, Hôpital Saint Antoine, AP-HP, Paris, France), Alexandre Mebazaa (Réanimation Chirurgicale, Hôpital Saint-Louis, AP-HP, Paris, France), Benjamin Chousterman (Réanimation, Hôpital Lariboisière, AP-HP, Paris, France), Ahmed El Kalioubie (Réanimation, CHU de Lille), Sophie Six (Réanimation, CHU de Lille, Lille, France), Pauline Coulon and Boualem Sendid (Service de Mycologie, CHU de Lille, France), Nadia Anguel (Réanimation Médicale, Le Kremlin-Bicêtre, AP-HP, Paris, France), Charles Damoisel (Réanimation Polyvalente, Clamart, AP-HP, France), Charlotte Mussini (Service d’Anatomopathologie, Le Kremlin-Bicêtre, AP-HP, Paris, France), Alban Villate (Hématologie, Tours, France), Jean-Christophe Navellou (Réanimation, CHU Besançon, France), Christophe Girault (Médecine Intensive et Réanimation, CHU Rouen, France), Carole Cassagne (Laboratoire de Mycologie, CHU Timone, Marseille, France), Olivier Augereau (Laboratoire de Microbiologie, Colmar, France), Francoise Dromer, Dea Garcia-Hermoso, Olivier Lortholary, Alexandre Alanio (CNRMA, Institut Pasteur, Paris, France)., Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), and Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille)

Subjects
medicine.medical_specialty, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, CAM, Coronavirus disease 2019 (COVID-19), business.industry, Brief Report, General surgery, Mucormycosis, COVID-19, CAPA, medicine.disease, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, mucormycosis, AcademicSubjects/MED00290, Infectious Diseases, Oncology, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, medicine, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, business, Complication
Abstract

We studied COVID-19 associated mucormycosis based on 17 cases reported nationwide and assessed the differences with India. They differed by frequencies of diabetes mellitus (47% in France versus up to 95% in India), hematological malignancies (35% versus 1%), anatomical sites (12% versus >80% rhino-orbito-cerebral) and prognosis (88% mortality versus, We reported 17 cases of COVID-19 associated mucormycosis in France. Compared to India, they differed by frequencies of diabetes mellitus (47% versus up to 95% in India), hematological malignancies (35% versus 1%), anatomical sites and mortality (88% versus

Published
2022
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23. Diagnosis of mucormycosis using an intercalating dye-based quantitative PCR

Author

Bigot, Jeanne, primary, Godmer, Alexandre, additional, Prudenté, Lysa, additional, Angebault, Cécile, additional, Brissot, Eolia, additional, Bige, Naike, additional, Voiriot, Guillaume, additional, Leger, Pierre-Louis, additional, Petit-Hoang, Camille, additional, Atallah, Sarah, additional, Gouache, Elodie, additional, Senghor, Yaye, additional, Valot, Stéphane, additional, Hennequin, Christophe, additional, and Guitard, Juliette, additional

Published
2022
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25. Invasive aspergillosis due to Aspergillus cryptic species: A prospective multicentre study

Author

Imbert, Sebastien, primary, Cassaing, Sophie, additional, Bonnal, Christine, additional, Normand, Anne‐Cecile, additional, Gabriel, Frederic, additional, Costa, Damien, additional, Blaize, Marion, additional, Lachaud, Laurence, additional, Hasseine, Lilia, additional, Kristensen, Lise, additional, Guitard, Juliette, additional, Schuttler, Christine, additional, Raberin, Helene, additional, Brun, Sophie, additional, Hendrickx, Marijke, additional, Piarroux, Renaud, additional, and Fekkar, Arnaud, additional

Published
2021
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26. Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces / Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study.

Author

Monpierre, Lorra, Aït-Ammar, Nawel, Valsecchi, Isabel, Normand, Anne-Cécile, Guitard, Juliette, Riat, Arnaud, Huguenin, Antoine, Bonnal, Christine, Sendid, Boualem, Hasseine, Lilia, Raberin, Hélène, Dehais, Marion, Ranque, Stéphane, Hennequin, Christophe, Piarroux, Renaud, Dannaoui, Eric, and Botterel, Françoise

Subjects
PAECILOMYCES, ANTIFUNGAL agents, SPECIES, CANDIDEMIA, AMPHOTERICIN B, MASS spectrometry, ECHINOCANDINS
Abstract

Paecilomyces spp. are emerging fungal pathogens, where Paecilomyces lilacinus and Paecilomyces variotii are the most reported species. Taxonomic and phylogenetic revisions in this genus have shown that P. variotii represents a species complex, whereas P. lilacinus is related to another genus called Purpureocillium. The aims of this study were to identify clinical isolates of Paecilomyces spp. at the species level, and to determine their antifungal susceptibility profiles. 70 clinical Paecilomyces spp. isolates were identified by MALDI-TOF Mass Spectrometry (MS) and by multilocus rDNA genes sequencing including ITS and the D1/D2 genes. Among the 70 Paecilomyces spp. isolates, 28 were identified as P. lilacinum, 26 as P. variotii stricto sensu, and 16 as P. maximus. For antifungal susceptibility testing, Minimal Inhibitory Concentrations (MICs) or Minimal Effective Concentrations (MECs) were determined for 8 antifungals. All P. lilacinum isolates had high MICs and MECs of amphotericin B and echinocandins, respectively, unlike P. variotii and P. maximus. For azole drugs, MICs were molecule- and species- dependent. The differences in in vitro susceptibility to antifungals underline the importance of accurate species identification. The MALDI–TOF MS can be a good alternative in routine laboratory to ensure fast identification of Paecilomyces spp. and P. lilacinum. [ABSTRACT FROM AUTHOR]

Published
2022
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28. Clinical Origin and Species Distribution of Fusarium spp. Isolates Identified by Molecular Sequencing and Mass Spectrometry: A European Multicenter Hospital Prospective Study

Author

Normand, Anne-Cécile, primary, Imbert, Sébastien, additional, Brun, Sophie, additional, Al-Hatmi, Abdullah M. S., additional, Chryssanthou, Erja, additional, Cassaing, Sophie, additional, Schuttler, Christine, additional, Hasseine, Lilia, additional, Mahinc, Caroline, additional, Costa, Damien, additional, Bonnal, Christine, additional, Ranque, Stéphane, additional, Sautour, Marc, additional, Rubio, Elisa, additional, Delhaes, Laurence, additional, Riat, Arnaud, additional, Sendid, Boualem, additional, Kristensen, Lise, additional, Brandenberger, Marcel, additional, Guitard, Juliette, additional, Packeu, Ann, additional, Piarroux, Renaud, additional, and Fekkar, Arnaud, additional

Published
2021
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30. High Prevalence of Fungal Infections in Mechanically Ventilated COVID-19 Patients in the ICU: The French Multicenter MYCOVID Study

Author

Gangneux, Jean Pierre, primary, Dannaoui, Eric, additional, Fekkar, Arnaud, additional, Luyt, Charles Edouard, additional, Botterel, Francoise, additional, de Prost, Nicolas, additional, Tadie, Jean Marc, additional, Reizine, Florian, additional, Houze, Sandrine, additional, Timsit, Jean-François, additional, Iriart, Xavier, additional, Riu-Poulenc, Béatrice, additional, Sendid, Boualem, additional, Nseir, Saad, additional, Persat, Florence, additional, Wallet, Florent, additional, Le Pape, Patrice, additional, Canet, Emmanuel, additional, Novara, Ana, additional, Manai, Melek, additional, Cateau, Estelle, additional, Thille, Arnaud W., additional, Brun, Sophie, additional, Cohen, Yves, additional, Alanio, Alexandre, additional, Megarbane, Bruno, additional, Cornet, Muriel, additional, Terzi, Nicolas, additional, Lamhaut, Lionel, additional, Sabourin, Estelle, additional, Desoubeaux, Guillaume, additional, Ehrmann, Stephan, additional, Hennequin, Christophe, additional, Voiriot, Guillaume, additional, Nevez, Gilles, additional, Aubron, Cécile, additional, Letscher-Bru, Valérie, additional, Meziani, Ferhat, additional, Blaize, Marion, additional, Mayaux, Julien, additional, Monsel, Antoine, additional, Boquel, Frederique, additional, Robert-Gangneux, Florence, additional, Le Tulzo, Yves, additional, Seguin, Philippe, additional, Guegan, Hélène, additional, Autier, Brice, additional, Lesouhaitier, Mathieu, additional, Pelletier, Romain, additional, Belaz, Sorya, additional, Bonnal, Christine, additional, Berry, Antoine, additional, Leroy, Jordan, additional, François, Nadine, additional, Richard, Jean-Christophe, additional, Paulus, Sylvie, additional, Argaud, Laurent, additional, Dupont, Damien, additional, Menotti, Jean, additional, Morio, Florent, additional, Soulié, Marie, additional, Schwebel, Carole, additional, Garnaud, Cécile, additional, Guitard, Juliette, additional, Le Gal, Solène, additional, Quinio, Dorothée, additional, Morcet, Jeff, additional, Laviolle, Bruno, additional, Zahar, Jean-Ralph, additional, and Bougnoux, Marie-Elisabeth, additional

Published
2021
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35. QPCR detection of Mucorales DNA in bronchoalveolar lavage fluid to diagnose pulmonary mucormycosis

Author

Scherer, Emeline, Iriart, Xavier, Bellanger, Anne-Pauline, Dupont, Damien, Guitard, Juliette, Gabriel, Frédéric, Cassaing, Sophie, Charpentier, Eléna, Guenounou, Sarah, Cornet, Murielle, Botterel, Françoise, Rocchi, Steffi, Berceanu, Ana, Millon, Laurence, Service de parasitologie et mycologie [CHRU de Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Service de Parasitologie et Mycologie, CHU Toulouse [Toulouse]-Institut Fédératif de Biologie (IFB) - Hôpital Purpan, Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en neurosciences de Lyon - Lyon Neuroscience Research Center (CRNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hospices Civils de Lyon (HCL), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Sorbonne Université (SU), CHU de Bordeaux Pellegrin [Bordeaux], Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Institut Universitaire du Cancer de Toulouse - Oncopole (IUCT Oncopole - UMR 1037), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Grenoble Alpes - UFR Médecine (UGA UFRM), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire de Parasitologie-Mycologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service d'hématologie, Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse], Centre de recherche en neurosciences de Lyon (CRNL), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Institut de Recherche pour le Développement (IRD), Laboratoire Chrono-environnement (UMR 6249) (LCE), Service de Parasitologie et Mycologie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), and Université de Toulouse (UT)-Université de Toulouse (UT)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Cutaneous mucormycosis, Lichtheimia species, Bronchoalveolar lavage, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, Mucorales qPCR, Bandage, Quantitative PCR, Diagnosis, Mucorales, Mucormycosis, Burn, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
Abstract

International audience; Early diagnosis and treatment are essential to improving the outcome of mucormycosis. The aim of this retrospective study was to assess the contribution of quantitative PCR detection of Mucorales DNA in bronchoalveolar lavage fluids for early diagnosis of pulmonary mucormycosis.Bronchoalveolar lavage fluids (n=450) from 374 patients with pneumonia and immunosuppressive conditions were analyzed using a combination of 3 quantitative PCR assays targeting the main genera involved in mucormycosis in France (Rhizomucor, Mucor/Rhizopus, Lichtheimia).Among these 374 patients, 24 had at least one bronchoalveolar lavage with a positive PCR; 23/24 patients had radiological criteria for invasive fungal infections according to consensual criteria : 10 patients with probable or proven mucormycosis, and 13 additional patients with other invasive fungal infections (4 probable aspergillosis, 1 proven fusariosis, and 8 possible invasive fungal infections). Only 2/24 patients with a positive PCR on bronchoalveolar lavage had a positive Mucorales culture.PCR was also positive on serum in 17/24 patients. In most cases, PCR was first detected positive on sera (15/17). However, a positive PCR on bronchoalveolar lavage was the earliest and/or the only biological test revealing mucormycosis in 4 patients with a final diagnosis of probable or proven mucormycosis, 3 patients with probable aspergillosis and one patient with a possible invasive fungal infection.Mucorales PCR performed on bronchoalveolar lavage could provide additional arguments for earlier administration of Mucorales-directed antifungal therapy, thus improving the outcome of lung mucormycosis.

Published
2018
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38. Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal

Author

Lund Ole, Fievet Nadine, Gnidehou Sédami, Ermont Caroline, Andersen Pernille, Guitard Juliette, Deloron Philippe, and Ndam Nicaise

Subjects
Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Pregnant women acquire protective antibodies that cross-react with geographically diverse placental Plasmodium falciparum isolates, suggesting that surface molecules expressed on infected erythrocytes by pregnancy-associated malaria (PAM) parasites have conserved epitopes and, that designing a PAM vaccine may be envisaged. VAR2CSA is the main candidate for a pregnancy malaria vaccine, but vaccine development may be complicated by its sequence polymorphism. Methods The dynamics of P. falciparum genotypes during pregnancy in 32 women in relation to VAR2CSA polymorphism and immunity was determined. The polymorphism of the msp2 gene and five microsatellites was analysed in consecutive parasite isolates, and the DBL5ε + Interdomain 5 (Id5) part of the var2csa gene of the corresponding samples was cloned and sequenced to measure variation. Results In primigravidae, the multiplicity of infection in the placenta was associated with occurrence of low birth weight babies. Some parasite genotypes were able to persist over several weeks and, still be present in the placenta at delivery particularly when the host anti-VAR2CSA antibody level was low. Comparison of diversity among genotyping markers confirmed that some PAM parasites may harbour more than one var2csa gene copy in their genome. Conclusions Host immunity to VAR2CSA influences the parasite dynamics during pregnancy, suggesting that the acquisition of protective immunity requires pre-exposure to a limited number of parasite variants. Presence of highly conserved residues in surface-exposed areas of the VAR2CSA immunodominant DBL5ε domain, suggest its potential in inducing antibodies with broad reactivity.

Published
2010
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39. Differential evolution of anti-VAR2CSA- IgG3 in primigravidae and multigravidae pregnant women infected by Plasmodium falciparum

Author

Sow Sokhna, Li Tengfei, Salanti Ali, Magnouha Nellie, Cottrell Gilles, Guitard Juliette, Deloron Philippe, and Tuikue Ndam Nicaise

Subjects
Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Pregnant women develop protective anti-VSA IgG1 and IgG3 when infected by Plasmodium falciparum. The major target of IgG from serum of infected pregnant women is VAR2CSA. Methods In this study, ELISA was used to compare the level of VAR2CSA DBL5ε- specific IgG subclasses at enrolment and at delivery in a cohort of pregnant women in Senegal. All antibody measures were analysed in relation to placental infection according to parity. Results The results show an interaction between immune response to placental malaria and parity. A higher level of anti- DBL5ε- IgG3 at enrolment and a higher increase between enrolment and delivery were found in primigravidae who presented with uninfected placenta at delivery in comparison to those who presented with an infection of the placenta. However, high antibody level at delivery was associated with the infection of the placenta in multigravidae. Conclusion This high level of IgG3 in uninfected primigravidae suggests a protective role of these antibodies in this susceptible group, highlighting the importance of VAR2CSA in general and of some of its variants still to be defined, in the induction of protective immunity to pregnancy malaria.

Published
2008
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40. Respiratory Epithelial Cells Can Remember Infection: A Proof-of-Concept Study.

Author

Bigot, Jeanne, Guillot, Loic, Guitard, Juliette, Ruffin, Manon, Corvol, Harriet, Chignard, Michel, Hennequin, Christophe, and Balloy, Viviane

Subjects
EPITHELIAL cells, HISTONE acetyltransferase, RESPIRATORY infections, IMMUNOLOGIC memory, PSEUDOMONAS aeruginosa
Abstract

Human bronchial epithelial cells play a key role in airway immune homeostasis. We hypothesized that these sentinel cells can remember a previous contact with pathogen compounds and respond nonspecifically to reinfection, a phenomenon called innate immune memory. We demonstrated that their preexposure to Pseudomonas aeruginosa flagellin modify their inflammatory response to a second, nonrelated stimulus, including live pathogens or lipopolysaccharide. Using histone acetyltransferase and methyltransferase inhibitors, we showed that this phenomenon relied on epigenetic regulation. This report is a major breakthrough in the field of multimicrobial respiratory tract infections, wherein control of inflammatory exacerbations is a major therapeutic issue. [ABSTRACT FROM AUTHOR]

Published
2020
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41. Quantitative PCR (qPCR) Detection of Mucorales DNA in Bronchoalveolar Lavage Fluid To Diagnose Pulmonary Mucormycosis

Author

Scherer, Emeline, primary, Iriart, Xavier, additional, Bellanger, Anne Pauline, additional, Dupont, Damien, additional, Guitard, Juliette, additional, Gabriel, Frederic, additional, Cassaing, Sophie, additional, Charpentier, Eléna, additional, Guenounou, Sarah, additional, Cornet, Murielle, additional, Botterel, Françoise, additional, Rocchi, Steffi, additional, Berceanu, Ana, additional, and Millon, Laurence, additional

Published
2018
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43. Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum

Author

de Almeida, João N., primary, Favero Gimenes, Viviane M., additional, Francisco, Elaine C., additional, Machado Siqueira, Lumena P., additional, Gonçalves de Almeida, Renato K., additional, Guitard, Juliette, additional, Hennequin, Christophe, additional, Colombo, Arnaldo L., additional, Benard, Gil, additional, and Rossi, Flavia, additional

Published
2017
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44. Micafungin susceptibility of the most common Candida species in 16 French university hospitals : comparison between the Etest® and the EUCAST methods (MICACAND study)

Author

Bougnoux, Marie-Elisabeth, Dannaoui, Eric, Accoceberry, Isabelle, Angoulevant, Adela, Bailly, E., Bouchara, Jean-Philippe, Botterel, Françoise, Chevrier, Sylviane, Chouaki, Taieb, Cornet, Muriel, Fekkar, Arnault, Dalle, Frédéric, Gangneux, Jean-Pierre, Guitard, Juliette, Hennequin, Christophe, Lepape, Patrice, Maubon, Danièle, Ranque, Stéphane, Sendid, Boualem, Chandenier, Jacques, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris], Unité de Parasitologie-Mycologie [CHU HEGP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Dynamic Microbiology - EA 7380 (DYNAMIC), École nationale vétérinaire - Alfort (ENVA)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), CHU de Bordeaux Pellegrin [Bordeaux], Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Hôpital Bretonneau, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM), Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), CHU Henri Mondor, CHU Pontchaillou [Rennes], CHU Amiens-Picardie, Université de Picardie Jules Verne (UPJV), CHU Grenoble, Service de Parasitologie - Mycologie [CHU Pitié-Salpétrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Hôpital du Bocage, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), CHU Saint-Antoine [AP-HP], Centre d'Immunologie et de Maladies Infectieuses (CIMI), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre hospitalier universitaire de Nantes (CHU Nantes), Cibles et médicaments de l'infection, de l'immunité et du cancer (IICiMed), Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes (UN)-Université de Nantes (UN), Assistance Publique - Hôpitaux de Marseille (APHM), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Centre Hospitalier Universitaire de Lille (CHU de Lille), Physiopathologie des Candidoses, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, Service de parasitologie, mycologie, médecine tropicale [CHRU Tours], European Congress of Clinical Microbiology and Infectious Deseases (ECCMID). INT., Biologie et Pathogénicité fongiques, Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Recherche Agronomique (INRA), Service de parasitologie - mycologie [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Microbiologie Fondamentale et Pathogénicité (MFP), CHU Henri Mondor [Créteil], Cibles et Médicaments des Infections et de l'Immunité (IICiMed), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)

Subjects
E-test, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, Micafungin, Candida, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, bacterial infections and mycoses
Abstract

EA MERS; International audience; Objectives : Micafungin is currently used in France. The aim of this study is to determine its activity against a recent (2014) French collection of Candida isolates. Although EUCAST is the reference method for in vitro antifungal susceptibility testing, it is not commonly used in routine clinical microbiology laboratories. Thus, it is important to evaluate alternative methods. We compared EUCAST and Etest for micafungin susceptibility testing of Candida spp. and we monitored the emergence of resistance. Methods: Sixteen centers (6 in Paris area and 10 across France) participated in a two-months prospective study. Clinical isolates of various Candida species (mainly C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, C. kefyr and C. krusei, about 10 isolates of each species per center) were tested by Etest, according to manufacturer’s instructions. All isolates were subsequently centralized in one center for MIC determination by EUCAST method. For comparison purposes, Etest MICs were raised to the next higher EUCAST concentration. Resistance was defined based on EUCAST clinical breakpoints or on epidemiological cut-off values when clinical breakpoints were not available. Results : A total number of 933 Candida isolates were tested. The overall agreement (+/- 2 log2 dilutions) between EUCAST and Etest was 97.9%. Species n E-Test EUCAST MIC range MIC range % agreement with Etest % resistance C. albicans 159 ≤ 0.015 - 0.06 ≤ 0.015 - 0.06 100 1.3 C. tropicalis 152 ≤ 0.015 - 0.5 ≤ 0.015 - 1 98.7 0.7 C. parapsilosis 152 ≤ 0.015 - 4 ≤ 0.125 - 4 96.1 1.3 C. glabrata 152 ≤ 0.015 - 0.125 ≤ 0.015 - 1 98.7 3.9 C. kefyr 136 ≤ 0.015 - 0.25 ≤ 0.015 - 0.125 97.8 ND C. krusei 127 ≤ 0.015 - 1 ≤ 0.015 - 0.25 96.9 0 Other Candida species* 55 ≤ 0.015 - 1 ≤ 0.015 - 1 94.5 ND Total 933 ≤ 0.015 - 4 ≤ 0.015 - 4 97.9 ND *: C. lusitaniae, C. guilliermondii, C. norvegensis, C. inconspicua, C. famata, C. pelliculosa, C. lambica, C. sphaerica, C. ciferii, C. catenulata, C. utilis, C. colliculosa, C. nivariensis Conclusions : This study demonstrated a very good agreement between Etest, performed on a routine basis, and EUCAST for micafungin MIC determination. Micafungin resistance among the main Candida species was uncommon.

Published
2015

45. Features of Toxoplasma gondiireactivation after allogeneic hematopoietic stem-cell transplantation in a high seroprevalence setting

Author

Paccoud, Olivier, Guitard, Juliette, Labopin, Myriam, Surgers, Laure, Malard, Florent, Battipaglia, Giorgia, Duléry, Rémy, Hennequin, Christophe, Mohty, Mohamad, and Brissot, Eolia

Abstract

We performed a single-centre retrospective study to evaluate the effectiveness of Toxoplasma gondiiprevention strategies after allogeneic stem-cell transplantation. The charts of 138 allogeneic stem-cell recipients over a 4-year period were reviewed. Forty-nine percent of patients were not receiving optimal trimethoprim–sulfamethoxazole (TMP–SMZ) prophylaxis at day +30, mainly due to persistent cytopenia. Six months after transplantation, the rate of toxoplasmosis reactivation was 11.6%, including nine cases of Toxoplasmainfection and seven cases of Toxoplasmadisease. Fifty-six percent of cases of reactivation occurred before day +30. Thirty-eight percent occurred in patients receiving atovaquone prophylaxis. In 57% of patients presenting with Toxoplasmadisease, signs of disease were present at first evidence of ToxoplasmaDNA in peripheral blood samples. This study illustrates the limitations inherent to currently used toxoplasmosis prevention strategies and argues for the use of a combined prophylactic and preemptive approach. After performing the initial study, we limited the use of atovaquone in favour of TMP–SMZ when possible, and implemented an early prevention strategy consisting of the introduction of prophylaxis starting on day of engraftment. Over the following 16 months, 88.9% of eligible Toxoplasma-seropositive patients were receiving TMP–SMZ at day +30, and the rate of early Toxoplasmareactivation was 1.5%.

Published
2020
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46. The role of the G6PD AEth376G/968C allele in glucose-6-phosphate dehydrogenase deficiency in the seerer population of Senegal

Author

de Araujo, Carla, Migot-Nabias, Florence, Guitard, Juliette, Pelleau, Stéphane, Vulliamy, Tom, Ducrocq, Rolande, Guitard, Juliette, Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (MERIT - UMR_D 216), Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5), Mucoviscidose: physiopathologie et phénogénomique [CRSA], Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques (IP-TPT), and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Service de Santé des Armées

Subjects
Male, MESH: Humans, Genotype, MESH: Alleles, [SDV]Life Sciences [q-bio], MESH: Glucosephosphate Dehydrogenase, MESH: Population Groups, Glucosephosphate Dehydrogenase, MESH: Male, Senegal, MESH: Genotype, MESH: Glucosephosphate Dehydrogenase Deficiency, [SDV] Life Sciences [q-bio], Glucosephosphate Dehydrogenase Deficiency, Gene Frequency, Population Groups, MESH: Senegal, MESH: Child, MESH: Gene Frequency, Humans, Female, Child, MESH: Female, Alleles
Abstract

International audience; Glucose-6-phosphate dehydrogenase (G6PD) deficiency is common in tropical Sub-Saharan countries. The allele most frequently associated with G6PD deficiency in this a region is G6PD 376G/202A. Here, we show that, the prevalence of G6PD deficiency is 12% in the Sereer ethnic group from Senegal ant that the 376G/968C genotype is predominant; the frequency of the 376G/202A genotype is very low in this ethnic group.

Published
2006

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