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Abstract Background and aims Evidence from prospective cohort studies on the relationship between metabolic dysfunction-associated steatotic liver disease (MASLD) and longitudinal changes in serum ferritin (SF) still limited. This study aimed to investigate the associations of SF baselines and trajectories with new-onset MASLD and to present a MASLD discriminant model. Methods A total of 1895 participants who attended health examinations at least three times in a hospital in Dalian City between 2015 and 2022 were included. The main outcome was the incidence of MASLD. The associations between SF baselines and trajectories with the risk of MASLD were analyzed by Cox proportional hazards regression, restricted cubic spline (RCS) analysis and time-dependent receiver operating characteristic (ROC) curve analysis. In addition, a MASLD discrimination model was established using logistic regression analyses. Results Among the 1895 participants, 492 developed MASLD during follow-up. Kaplan-Meier analysis indicated that participants in the low-stable trajectory group had a longer MASLD-free time compared with participants in other groups. Compared with those in the low-stable trajectory group, the adjusted hazard ratios (HRs) with 95% confidence intervals (CIs) for the risk of new-onset MASLD in the medium-high, high-stable and high-high trajectory groups were 1.54(1.18-2.00), 1.77(1.35–2.32) and 1.55(1.07–2.26), respectively (P trend < 0.001). The results were robust in subgroup and sensitivity analyses. Multivariate Cox proportional regression showed that SF was an independent risk factor of MASLD (HR = 1.002, 95%CI: 1.000-1.003, P = 0.003). The restricted cubic spline demonstrated a nonlinear relationship between SF and the risk of MASLD. The 8-variable model had high discriminative performance, good accuracy and clinical effectiveness. The ROC curve results showed that AUC was greater than that of the FLI, HSI and ZJU models (all P

BackgroundPrevious studies on the liner associations between serum 25-hydroxyvitamin D [25(OH)D] levels and lipid profiles revealed ambiguous findings. The current study therefore tried to elucidate the possible non-linear associations between 25(OH)D and lipid profiles.MethodsThis study involved 8,516 adult participants (aged 18–74 years, males N = 3,750, females N = 4,766) recruited from the Dalian health management cohort (DHMC). The risk (OR) for specific dyslipidemias was estimated across the serum 25(OH)D levels and the cut-off value for serum 25(OH)D were determined by using logistic regression, restricted cubic spline, and piecewise linear regression methods, adjusted for age, sex, season, and ultraviolet index.ResultsIn this study, a high prevalence of 25(OH)D deficiency was observed in the participants (65.05%). The level of 25(OH)D showed the inverse U-shaped correlations with the risks (ORs) of abnormal lipid profile, with inflection points observed at 23.7 ng/ml for hypercholesterolemia, 24.3 ng/ml for hypertriglyceridemia, 18.5 ng/ml for hyper-low-density lipoprotein cholesterolemia, 23.3 ng/ml for hypo-high-density lipoprotein cholesterolemia, 23.3 ng/ml for hyper-non-high-density lipoprotein cholesterol, and 24.3 ng/ml for high remnant cholesterol. The stratified analyses showed that the risk for most dyslipidemias related to deficiency of 25(OH)D was particularly increased among females aged 50–74 (except for hypertriglyceridemia, where the highest risk was among men aged 50–74 years), during winter/spring or under low/middle ultraviolet index environments.ConclusionsNonlinear inverse U-shaped associations were observed between 25(OH)D levels and abnormal lipid profile. The risk was particularly increased among females aged 50-74, during winter/spring period or under lower ultraviolet index environments. In vitamin D deficient subjects [25(OH)D

ObjectiveTo examine the prevalence of malignant tumors among residents in Shaanxi province in 2019. MethodsAll hospitalizations due to malignant tumors throughout 2019 were extracted from inpatient medical records of hospitals in Shaanxi povince. The International Classification of Diseases, 10th Revision (ICD-10) was used to categorize the inpatients′ malignant tumors and multiple imputation method was adopted to adjust the covariates with missing data. National and provincial demographics were collected from National Bureau of Statistics and Statistics Yearbook of Shaanxi province for calculating age-adjusted prevalence rate. ResultsThe overall prevalence rate (1/100 000) of malignant tumors in the province during 2019 was 392.38; the highest regional prevalence rate was 523.68 for the residents of Tongchuan prefecture, while the lowest was 34.256 for those of Weinan prefecture; and the highest tumor-specific prevalence rate was 144.21 for digestive malignancies. In the male residents of the province, the top three prevalence rates were 187.50, 106.25, and 31.05 for malignancies of digestive organs, respiratory and intrathoracic organs, and lymphoid, hematopoietic and related tissues; in the female residents, the top three prevalence rates were 98.04, 70.60, and 67.18 for malignancies of digestive organs, breast, and female reproductive organs; the top three prevalence rates were 109.72, 58.15, and 30.13 for malignancies of digestive organs, respiratory and intrathoracic organs, and breast in the urban residents of the province, but in the rural residents, the top three prevalence rates were 194.32, 100.14, and 45.37 for malignancies of digestive organs, respiratory and intrathoracic organs, and female reproductive organs, respectively. The prevalent malignancies differed among the residents of various age groups and the age-specific prevalence rate of malignancies was the highest (62.89) for the residents aged 65 – 69 years. ConclusionAmong the residents in Shaanxi province during 2019, the overall prevalence rate of malignancies was relatively high and malignant tumor of digestive organs was the most prevalent malignancy; the prevalence rates of malignancies differed among the residents of different gender, various ages, and living in different regions; the results need to be concerned in early screening and control of malignant tumors in the province.

Six mulberry varieties in Shandong Province were used to investigate their quality, physiological properties and volatile aroma components by sensory evaluation, physicochemical analysis and gas chromatography-mass spectrometry techniques. The results showed that “Baiyuwang” and “Dashi” had the highest weight and shape index. “Heizhenzhu”and “Moyu” appeared the highest hardness (3.32, 2.67 kg/cm2) and solid-acid ratio (92.87, 82.76), as well as higher polygalacturonase (PG) and lipoxygenase (LOX) activity. But the higher juice yield (63.32%, 61.19%) was found in “Moyu” and “Dashi”. In addition, “Dashi” and “Hongguo” had higher anthocyanin (0.58, 0.53 mg/g), total phenolic content (2.48, 2.59 mg/g), strong DPPH and ABTS+ free radical scavenging ability, followed by “Longguai”, “Moyu” and “Heizhenzhu”, while “Baiyuwang” was the lowest in anthocyanin and total phenolic. The analysis of volatile aroma components showed that “Moyu” had the most aroma species and reached 62, followed by “Dashi” and “Baiyuwang”. Among them, the aroma components of “Heizhenzhu” and “Dashi” were mainly alcohols, however, “Moyu”, “Longguai” and “Baiyuwang” were mainly aldehydes, ketones and olefins. The common aroma components of the six varieties were mainly 2-amino-6-methylbenzoic acid and hexanal. The study provides a reference for the quality evaluation, processing and utilization of mulberry varieties.

OBJECTIVE To investigate whether the interaction between serum vitamin D and serum uric acid has an effect on blood pressure. METHODS A cross-sectional study was conducted with 7,951 subjects selected from the health management center of the Second Hospital of Dalian Medical University during 1 January 2020 and 30 September 2022. The dose–response relationship between serum vitamin D, blood uric acid, and blood pressure was analyzed using restricted cubic spline. The relationship between each variable and elevated blood pressure was analyzed using multifactorial analysis in a generalized additive model and combined with a multiplicative–additive hierarchical interaction model to explore the effects of the interaction of serum vitamin D and blood uric acid on elevated blood pressure. RESULTS The mean age of the study population was (42.61 ± 11.17) years and the detection rate of hypertension was 12.14% (965/7,951). The risk of elevated blood pressure (high-normal blood pressure + hypertension) decreased with increasing serum vitamin D concentration, but the risk of elevated blood pressure increased with increasing serum uric acid levels. Compared with those with adequate serum vitamin D and normal blood uric acid, the odds ratio (OR) for the risk of high-normal blood pressure was 1.54 [95% confidence interval, CI (1.22, 1.94), P  < 0.001] and the OR for the risk of hypertension was 3.17 [95% CI (2.13, 4.72), P  < 0.001] for those with inadequate serum vitamin D and high blood uric acid. The risk of elevated blood pressure was highest when serum 25-hydroxyvitamin D was less than 25 nmol/l and blood uric acid was higher than 600 µmol/l. CONCLUSIONS The interaction between serum vitamin D and blood uric acid has an effect on elevated blood pressure. The risk of hypertension was maximized when serum vitamin D was insufficient and blood uric acid was high. [ABSTRACT FROM AUTHOR]

To unravel the roles of soluble acid invertase in muskmelon (Cucumis melo L.), its activity in transgenic muskmelon plants was reduced by an antisense approach. For this purpose, a 1038 bp cDNA fragment of muskmelon soluble acid invertase was expressed in antisense orientation behind the 35S promoter of the cauliflower mosaic virus. The phenotype of the antisense plants clearly differed from that of control plants. The transgenic plant leaves were markedly smaller, and the stems were obviously thinner. Transmission electron microscopy revealed that degradation of the chloroplast membrane occurred in transgenic leaves and the number of grana in the chloroplast was significantly reduced, suggesting that the slow growth and weaker phenotype of the transgenic plants may be due to damage to the chloroplast ultrastructure, which in turn resulted in a decrease in net photosynthetic rate. The sucrose concentration increased and levels of acid invertase decreased in transgenic fruit, and the fruit size was 60% smaller than that of the control. In addition, transgenic fruit reached full-slip at 25 d after pollination (DAP), approximately 5 d before the control fruit (full-slip at 30 DAP), and this accelerated maturity correlated with a dramatic elevation of ethylene production at the later stages of fruit development. Together, these results suggest that soluble acid invertase not only plays an important role during muskmelon plant and fruit development but also controls the sucrose content in muskmelon fruit. [ABSTRACT FROM PUBLISHER]