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Made available in DSpace on 2022-04-28T19:46:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) In recent years, the use of prescribed and non-prescribed drugs has increased. Therefore, advances in new technologies and sensors for detecting molecules in natural environments are required. In this work, a 3D-printed polylactic acid stencil is used to fabricate paper-based analytical devices (ePADs). Herein, we report the use of carbon-based lab-manufactured conductive ink for the fabrication of sensors towards the detection of chloroquine and escitalopram. For each batch, eight ePADs were successfully fabricated. Firstly, the fabricated sensors were evaluated morphologically by scanning electron microscopy and electrochemically by cyclic voltammetry and electrochemical impedance spectroscopy experiments. The sensors displayed a well-defined voltammetric profile in the presence of the redox couple, when compared to a commercial carbon screen-printed electrode. Differential pulse voltammetry conducted the detection of chloroquine and escitalopram with detection limits of 4.0 and 0.5 µmol L−1, respectively. The ePADs fabricated using the 3D stencil are here presented as alternatives for the fabrication of electrochemical analytical devices. Department of Bioprocess and Bioengineering School of Agriculture São Paulo State University (UNESP), Avenida Universitária, no. 3780, SP Department of Bioprocess and Bioengineering School of Agriculture São Paulo State University (UNESP), Avenida Universitária, no. 3780, SP FAPESP: 2017/24274-3

O ensino de conceitos físicos abordando o tema ondas eletromagnéticas é um dos grandes desafios para os professores de Ciências da Natureza no ensino fundamental, uma vez que esses professores geralmente são graduados em curso de Ciências Biológicas. Nesse contexto, o objetivo deste trabalho é apresentar um aprimoramento do objeto educacional conhecido como caixa de cores, no qual a fonte de luz é capaz de emular todo o espectro eletromagnético visível em função do comprimento de onda. É esperado que, com o uso desse objeto educacional, o estudante do ensino fundamental possa perceber que o espectro eletromagnético visível é contínuo e que cada comprimento de onda está associado a uma cor desse espectro. A caixa de cores foi construída utilizando um microcontrolador e componentes eletrônicos de baixo custo. O aparato proposto foi capaz de emular o espectro eletromagnético visível (de 400 nm até 700 nm), o que o torna inovador frente às propostas de caixa de cores já reportadas na literatura. O uso do objeto educacional permite a proposição de atividades didáticas abordando conceitos de ondas eletromagnéticas, assim como estudar padrões de absorção e reflexão dessas ondas por objetos de diferentes cores.

Made available in DSpace on 2021-06-25T11:04:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-01 The skin prick test is used to diagnose patients' sensitization to antigens through a mediated IgE response. It is a practical and quick exam, but its diagnosis depends on instruments for measuring the allergic response and observer's interpretation. The conventional method for inferring about the allergic reaction is performed from the dimensions of the wheals, which are measured using a ruler or a caliper. To make this diagnosis less dependent on human interpretation, the present study proposes two alternative methods to infer about the allergic reaction: computational determination of the wheal area and a study of the temperature variation of the patient's skin in the puncture region. For this purpose, prick test using histamine was performed on 20 patients randomly selected. The areas were determined by the conventional method using the dimensions of the wheals measured with a digital caliper 30 min after the puncture. The wheal areas were also determined by a Python algorithm using photographs of the puncture region obtained with a smartphone. A variable named circularity deviation was also determined for each analyzed wheal. The temperature variation was monitored using an infrared temperature sensor, which collected temperature data for 30 min. All results were statistically compared or correlated. The results showed that the computational method to infer the wheal areas did not differ significantly from the areas determined by the conventional method (p-value = 0.07585). Temperature monitoring revealed that there was a consistent temperature increase in the first minutes after the puncture, followed by stabilization, so that the data could be adjusted by a logistic equation (R2 = 0.96). This adjustment showed that the optimal time to measure the temperature is 800 s after the puncture, when the temperature stabilization occurs. The results have also shown that this temperature stabilization has a significant positive correlation with wheal area (p-value = 0.0015). Thus, we concluded that the proposed computational method is more accurate to infer the wheal area when compared to the traditional method, and that the temperature may be used as an alternative parameter to infer about the allergic reaction. Medical School Sao Paulo State University (UNESP), Prof. Mário Rubens Guimarães Montenegro Avenue, s/n Department of Bioprocess and Biotechnology School of Agriculture Sao Paulo State University (UNESP), 3780 Universitária Avenue School of Engineering Sao Paulo State University (UNESP), 14-01 Eng. Luiz Edmundo Carrijo Coube Avenue Medical School Sao Paulo State University (UNESP), Prof. Mário Rubens Guimarães Montenegro Avenue, s/n Department of Bioprocess and Biotechnology School of Agriculture Sao Paulo State University (UNESP), 3780 Universitária Avenue School of Engineering Sao Paulo State University (UNESP), 14-01 Eng. Luiz Edmundo Carrijo Coube Avenue

One of the ways to optimize animal production environments is through the understanding of the bioclimatic factors that are harmful to the animals. Among the techniques currently applied for measuring and analyzing bioclimatic factors, the vast majority demand that the producers and researchers have high level of technical skills, those techniques (e.g. using maps of spatial variability) hinder the rapid evaluation of certain environments. It is important to note that there is currently no software and hardware tool that allows for an on-site bioclimatic analysis, for example using an Android or iOS app. Thus, the present study was conducted with the objective of developing a system capable of collecting air temperature measurements ( T a ), black globe temperature ( T g ) and relative humidity ( R H ), to interact with Aurora mobile app, enabling the on-site development of maps with spatial variability of the air temperature and indices of thermal broilers comfort, using data interpolation. The system was developed through the creation of six transmitters and one receiver. The results obtained indicate that the system operated correctly and allows on-site analysis of thermal animal comfort. During the validation period a total of 48 maps were developed, considering the periods of morning and afternoon, and using the T a , B G H I and T H I measurements. The maps allowed the on-site identification of regions with conditions of comfort, discomfort and thermal stress (both by heat and cold). According to the maps of thermal comfort indices, the afternoon period presented conditions that were often heat stress. The maps of air temperature and thermal comfort indices allow the producers and researchers to have, at hand, a highly portable tool for evaluating the broilers welfare.

The literature has shown that there is no consensus regarding the best resin composite photoactivation protocol. This study evaluated the efficiency of the conventional, soft-start, pulse-delay and exponential protocols for photoactivation of resin composites in reducing the shrinkage stress and temperature variation during the photopolymerisation. The photoactivation processes were performed using a photocuring unit and a smartphone app developed to control the irradiance according each photoactivation protocol. These photoactivation methods were evaluated applying photoactivation energies recommended by the resins manufactures. Three brands of resin composites were analysed: Z-250, Charisma and Ultrafill. The cure effectiveness was evaluated through depth of cure experiments. All results were statistically evaluated using one-way and multi-factor analysis of variance (ANOVA). The use of exponential and pulse-delay methods resulted in a significant reduction of the shrinkage stress for all evaluated resins, however, the pulse-delay method required too long a photoactivation time. The increases on the temperature were lower when the exponential photoactivation was applied, however, the temperature variation for all photoactivation protocols was not enough to cause damage in the restoration area. The evaluation of the depth of cure showed that all photoactivation protocols resulted in cured resins with equivalent hardness, indicating that the choice of an alternative photoactivation protocol did not harm the polymerisation. In this way, the results showed the exponential protocol as the best photoactivation technique for practical applications.

Made available in DSpace on 2021-06-25T10:26:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Teaching and learning strategies that use innovating technologies, such as the development of software or an understanding of the theoretical concepts of analytical equipment, can be essential tools for the construction of knowledge. In this way, this work describes the construction of an innovative and lowcost colorimeter using a RGB LED and a microcontroller board as a guide for teachers of undergraduate classes to prepare pedagogical activities using the proposed device as a learning object. The device was applied in experiments using methylene blue (MB), methylene orange (MO) and cobalt chloride (CC) aqueous solutions of different concentrations. Each one of these solutions has absorption peaks near the RGB colors. The results demonstrated that this lowcost device was able to identify unambiguous absorption peaks in the expected range of the MB, MO and CC absorption spectrums, allowing us to determine a feasible calibration curve for each tested solution. Universidade Estadual Paulista “Júlio de Mesquista Filho” (UNESP). Professor Assistente Doutor Departamento de Bioprocessos e Biotecnologia São Paulo State University (UNESP) School of Agriculture Department of Bioprocess and Biotechnology Avenida Universitária São Paulo State University (UNESP) School of Medicine Avenida Prof. Mário Rubens Guimarães, Montenegro s/n São Paulo State University (UNESP) Institute of Biosciences Department of Physics and Biophysics Rua Prof. Dr. Antônio Celso Wagner Zanin Universidade de São Paulo (USP) Instituto de Física de São Carlos, Caixa Postal 369 Universidade Estadual Paulista “Júlio de Mesquista Filho” (UNESP). Professor Assistente Doutor Departamento de Bioprocessos e Biotecnologia São Paulo State University (UNESP) School of Agriculture Department of Bioprocess and Biotechnology Avenida Universitária São Paulo State University (UNESP) School of Medicine Avenida Prof. Mário Rubens Guimarães, Montenegro s/n São Paulo State University (UNESP) Institute of Biosciences Department of Physics and Biophysics Rua Prof. Dr. Antônio Celso Wagner Zanin