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1. Grain endogenous selenium and moderate salt stress work as synergic elicitors in the enrichment of bioactive compounds in maize sprouts

Author

Benincasa, P., D'Amato, R., Falcinelli, B., Troni, E., Fontanella, Maria Chiara, Frusciante, S., Guiducci, M., Beone, Gian Maria, Businelli, D., Diretto, G., Fontanella M. C. (ORCID:0000-0002-7667-0532), Beone G. M. (ORCID:0000-0002-5124-860X), Benincasa, P., D'Amato, R., Falcinelli, B., Troni, E., Fontanella, Maria Chiara, Frusciante, S., Guiducci, M., Beone, Gian Maria, Businelli, D., Diretto, G., Fontanella M. C. (ORCID:0000-0002-7667-0532), and Beone G. M. (ORCID:0000-0002-5124-860X)

Abstract

Salt stress and selenium are known to elicitate the production of plant secondary metabolites with antioxidant properties. On this basis, maize grains obtained from mother plants fertilized or not fertilized with selenium were sprouted at different levels of salinity (0, 25, and 50 mM NaCl) to evaluate the effects on the sprout yield, inorganic and organic Se species, minerals, and secondary metabolites, as revealed by a metabolomics analysis. Grain endogenous selenium (135 mg kg-1 vs. 0.19 mg kg-1 of the non-enriched grain) and salinity affected the sprout yield and composition, with salinity having the greatest effect on secondary metabolites. Most of the Se in sprouts was in an inorganic form, despite Se-enriched grains only containing organic Se. Some synergic effect was observed between Se and salinity. The best combination was obtained with Se-enriched grains sprouted at 25 mM NaCl, since this provided a good yield (not lower than in the untreated control), while sprout shoots were enriched in selenocystine and pro-nutritional semipolar compounds with antioxidant properties. Therefore, using grains from Se-fertilized crops and sprouting them under mild salt stress might represent a promising technique for improving the nutritional value of sprouts.

Published
2020

7. The α2δ subunit and absence epilepsy: Beyond calcium channels?

Author

Celli, R., Santolini, I., Guiducci, M., Luijtelaar, E.L.J.M. van, Parisi, P., Striano, P., Gradini, R., Battaglia, G., Ngomba, R.T., Nicoletti, F., Celli, R., Santolini, I., Guiducci, M., Luijtelaar, E.L.J.M. van, Parisi, P., Striano, P., Gradini, R., Battaglia, G., Ngomba, R.T., and Nicoletti, F.

Abstract

Contains fulltext : 175325.pdf (Publisher’s version ) (Open Access), Spike-wave discharges, underlying absence seizures, are generated within a cortico-thalamo-cortical network that involves the somatosensory cortex, the reticular thalamic nucleus, and the ventrobasal thalamic nuclei. Activation of T-type voltage-sensitive calcium channels (VSCCs) contributes to the pathological oscillatory activity of this network, and some of the first-line drugs used in the treatment of absence epilepsy inhibit T-type calcium channels. The α2δ subunit is a component of high voltage-activated VSCCs (i.e., L-, N-, P/Q-, and R channels) and has also been found to be associated with T channels. The presence of the α2δ subunit facilitates VSCC activation. Hence, one expects that drugs that bind to, and inhibit the α2δ subunit, e.g. gabapentin and pregabalin, are protective against absence epilepsy and that mice lacking the α2δ subunit are resistant to evoked absence seizures. In contrast, gabapentin and pregabalin are not clinically useful and may even be detrimental in the treatment of absence epilepsy, and ducky mice lacking the α2δ subunit develop absence seizures. This suggests that the α2δ subunit displays functions that go beyond the regulation of VSCCs, and that these functions are involved in the regulation of the cortico-thalamo-cortical network. This viewpoint critically examines the role of the α2δ subunit in the pathophysiology of absence seizures focusing on the potential role of the α2δ ligands, thrombospondins.

Published
2017

8. Alterations in the α2δ ligand, thrombospondin-1, in a rat model of spontaneous absence epilepsy and in patients with idiopathic/genetic generalized epilepsies

Author

Santolini, I., Celli, R., Cannella, M., Imbriglio, T., Guiducci, M., Parisi, P., Schubert, J., Iacomino, M., Zara, F., Lerche, H., Moyanova, S.G., Ngomba, R.T., Luijtelaar, E.L.J.M. van, Battaglia, G., Bruno, V., Striano, P., Nicoletti, F., Santolini, I., Celli, R., Cannella, M., Imbriglio, T., Guiducci, M., Parisi, P., Schubert, J., Iacomino, M., Zara, F., Lerche, H., Moyanova, S.G., Ngomba, R.T., Luijtelaar, E.L.J.M. van, Battaglia, G., Bruno, V., Striano, P., and Nicoletti, F.

Abstract

Contains fulltext : 178418.pdf (publisher's version ) (Open Access), Objectives: Thrombospondins, which are known to interact with the α2δ subunit of voltage-sensitive calcium channels to stimulate the formation of excitatory synapses, have recently been implicated in the process of epileptogenesis. No studies have been so far performed on thrombospondins in models of absence epilepsy. We examined whether expression of the gene encoding for thrombospondin-1 was altered in the brain of WAG/Rij rats, which model absence epilepsy in humans. In addition, we examined the frequency of genetic variants of THBS1 in a large cohort of children affected by idiopathic/genetic generalized epilepsies (IGE/GGEs). Methods: We measured the transcripts of thrombospondin-1 and α2δ subunit, and protein levels of α2δ, Rab3A, and the vesicular glutamate transporter, VGLUT1, in the somatosensory cortex and ventrobasal thalamus of presymptomatic and symptomatic WAG/Rij rats and in two control strains by real-time polymerase chain reaction (PCR) and immunoblotting. We examined the genetic variants of THBS1 and CACNA2D1 in two independent cohorts of patients affected by IGE/GGE recruited through the Genetic Commission of the Italian League Against Epilepsy (LICE) and the EuroEPINOMICS-CoGIE Consortium. Results: Thrombospondin-1 messenger RNA (mRNA) levels were largely reduced in the ventrobasal thalamus of both presymptomatic and symptomatic WAG/Rij rats, whereas levels in the somatosensory cortex were unchanged. VGLUT1 protein levels were also reduced in the ventrobasal thalamus of WAG/Rij rats. Genetic variants of THBS1 were significantly more frequent in patients affected by IGE/GGE than in nonepileptic controls, whereas the frequency of CACNA2D1 was unchanged. Significance: These findings suggest that thrombospondin-1 may have a role in the pathogenesis of IGE/GGEs.

Published
2017

9. Micotossine: nessun problema nel grano duro biologico

Author

Quaranta, F, Melloni, S, Desiderio, E, Aureli, G, D’Egidio, MG, Amoriello, T, Perenzin, M, Piazza, C, Reggiani, R, Bottazzi, P, Guiducci, M, Onofri, A, Petrini, A, Fuselli, D, Mazzieri, G, Santilocchi, R, Belocchi, A, Fornara, M, Basili, O, Piccioni, C, Codianni, P, Tedone, L, Lombardo GM, Gallo, G, AMATO, Gaetano, GIAMBALVO, Dario, Quaranta, F, Melloni, S, Desiderio, E, Aureli, G, D’Egidio, MG, Amoriello, T, Perenzin, M, Piazza, C, Reggiani, R, Bottazzi, P, Guiducci, M, Onofri, A, Petrini, A, Fuselli, D, Mazzieri, G, Santilocchi, R, Belocchi, A, Fornara, M, Basili, O, Piccioni, C, Codianni, P, Tedone, L, Lombardo GM, Gallo, G, Amato, G, and Giambalvo, D

Subjects
micotossine, Frumento duro
Published
2009

10. Rese e qualità di varietà di grano duro in biologico

Author

Quaranta, F, Belocchi, A, Camerini, M, D'Egidio, MG, Fornara, M, Pucciarmati, S, Melloni, S, Dettori, M, Lendini, M, Bottazzi, P, Mazzieri, G, Mazzoncini, M, Belloni, P, Antichi, D, Petrini, A, Fuselli, D, Antonelli, M, Codianni, P, Paone, S, Perenzin, M, Notario, T, Mazzon, V, Piazza, C, Reggiani, R, Lombardo, GM, Raciti, CN, Doust, MA, Virgillito, S, Guiducci, M, Santilocchi, R, Beldomenico, I, Bianchelli, M, Tedone, L, Gallo, G, Motta, V, Basili, O, Piccioni, C., AMATO, Gaetano, FRENDA, Alfonso Salvatore, GIAMBALVO, Dario, Quaranta, F, Belocchi, A, Camerini, M, D'Egidio, MG, Fornara, M, Pucciarmati, S, Melloni, S, Dettori, M, Lendini, M, Bottazzi, P, Mazzieri, G, Mazzoncini, M, Belloni, P, Antichi, D, Petrini, A, Fuselli, D, Antonelli, M, Codianni, P, Paone, S, Perenzin, M, Notario, T, Mazzon, V, Piazza, C, Reggiani, R, Lombardo, GM, Raciti, CN, Doust, MA, Virgillito, S, Amato, G, Frenda, AS, Giambalvo, D, Guiducci, M, Santilocchi, R, Beldomenico, I, Bianchelli, M, Tedone, L, Gallo, G, Motta, V, Basili, O, and Piccioni, C

Subjects
Triticum durum, durum wheat, organic farming, Settore AGR/02 - Agronomia E Coltivazioni Erbacee
Published
2009

11. Le varietà per il duro biologico

Author

QUARANTA F, BELOCCHI A, CAMERINI M, D'EGIDIO MG, FORNARA M, MELLONI S, DETTORI M, LENDINI M, BOTTAZZI P, GALANTE V, SILVERI D, MAZZIERI G, MAZZONCINI M, BELLONI P, ANTICHI D, PETRINI A, FUSELLI D, ANTONELLI M, CODIANNI P, PAONE S, PUCCIARMATI S, PERENZIN M, NOTARIO T, MAZZON V, PIAZZA C, REGGIANI R, LOMBARDO GM, RACITI CN, DOUST MA, VIRGILLITO S, GUIDUCCI M, BOLDRINI A, SANTILOCCHI R, BELDOMENICO I, BIANCHELLI M, TEDONE L, GALLO G, MOTTA V, BASILI O, PICCIONI C., AMATO, Gaetano, FRENDA, Alfonso Salvatore, GIAMBALVO, Dario, QUARANTA F, BELOCCHI A, CAMERINI M, D'EGIDIO MG, FORNARA M, MELLONI S, DETTORI M, LENDINI M, BOTTAZZI P, GALANTE V, SILVERI D, MAZZIERI G, MAZZONCINI M, BELLONI P, ANTICHI D, PETRINI A, FUSELLI D, ANTONELLI M, CODIANNI P, PAONE S, PUCCIARMATI S, PERENZIN M, NOTARIO T, MAZZON V, PIAZZA C, REGGIANI R, LOMBARDO GM, RACITI CN, DOUST MA, VIRGILLITO S, AMATO G, FRENDA A S, GIAMBALVO D, GUIDUCCI M, BOLDRINI A, SANTILOCCHI R, BELDOMENICO I, BIANCHELLI M, TEDONE L, GALLO G, MOTTA V, BASILI O, and PICCIONI C

Subjects
Triticum durum, agricoltura biologica
Published
2008

12. Rese e qualità del grano duro in coltivazione biologica

Author

QUARANTA F, BELOCCHI A, D'EGIDIO MG, FORNARA M, MAZZON V, MELLONI S, DESIDERIO E, BOTTAZZI P, GALANTE V, SILVERI D, MAZZIERI G, PETRINI A, FUSELLI D, ANTONELLI M, LOMBARDO M. G, RACITI CN, DOUST MA, VIRGILLITO S, GUIDUCCI M, BOLDRINI A, BONCIARELLI U, SANTILOCCHI R, BELDOMENICO I, BIANCHELLI M, CODIANNI P, PUCCIARMATI S, DUBOIS M, PERENZIN M, NOTARIO T, BASILI O, PICCIONI C., AMATO, Gaetano, FRENDA, Alfonso Salvatore, GIAMBALVO, Dario, QUARANTA F, BELOCCHI A, D'EGIDIO MG, FORNARA M, MAZZON V, MELLONI S, DESIDERIO E, BOTTAZZI P, GALANTE V, SILVERI D, MAZZIERI G, PETRINI A, FUSELLI D, ANTONELLI M, LOMBARDO M G, RACITI CN, DOUST MA, VIRGILLITO S, AMATO G, FRENDA A S, GIAMBALVO D, GUIDUCCI M, BOLDRINI A, BONCIARELLI U, SANTILOCCHI R, BELDOMENICO I, BIANCHELLI M, CODIANNI P, PUCCIARMATI S, DUBOIS M, PERENZIN M, NOTARIO T, BASILI O, and PICCIONI C

Published
2007

13. Varietà di grano duro a confronto in biologico

Author

QUARANTA F, BELOCCHI A, DEGIDIO MG, FORNARA M, MAZZON V, MELLONI S, DESIDERIO E, BOTTAZZI P, GALANTE V, SILVERI D, MAZZIERI G, PIAZZA C, REGGIANI R, MAZZONCINI M, BELLONI P, ANTICHI D, PETRINI A, FUSELLI D, LOMBARDO M. G, RACITI CN, DOUST MA, VIRGILLITO S, GUIDUCCI M, BOLDRINI A, BONCIARELLI U, SANTILOCCHI R, BELDOMENICO I, BIANCHELLI M, CODIANNI P, PUCCIARMATI S, DUBOIS M, PERENZIN M, NOTARIO T, BASILI O, PICCIONI C., AMATO, Gaetano, FRENDA, Alfonso Salvatore, GIAMBALVO, Dario, QUARANTA F, BELOCCHI A, DEGIDIO MG, FORNARA M, MAZZON V, MELLONI S, DESIDERIO E, BOTTAZZI P, GALANTE V, SILVERI D, MAZZIERI G, PIAZZA C, REGGIANI R, MAZZONCINI M, BELLONI P, ANTICHI D, PETRINI A, FUSELLI D, LOMBARDO M G, RACITI CN, DOUST MA, VIRGILLITO S, AMATO G, FRENDA A S, GIAMBALVO D, GUIDUCCI M, BOLDRINI A, BONCIARELLI U, SANTILOCCHI R, BELDOMENICO I, BIANCHELLI M, CODIANNI P, PUCCIARMATI S, DUBOIS M, PERENZIN M, NOTARIO T, BASILI O, and PICCIONI C

Published
2006

14. Annata critica per il tenore proteico del frumento duro biologico

Author

QUARANTA F, BELOCCHI A, DEGIDIO MG, FORNARA M, MAZZON V, DESIDERIO E, BOTTAZZI P, SILVERI D, GALANTE V, MAZZIERI G, PIAZZA C, REGGIANI R, BELLONI P, ANTICHI D, PETRINI A, FUSELLI D, LOMBARDO M. G, RACITI CN, DOUST MA, VIRGILLITO S, DE FRANCHI AS, LANDI G, GUIDUCCI M, BOLDRINI A, SANTILOCCHI R, BIANCHELLI M, CAMPI P, SPINA A, CAMBREA M, CODIANNI P, GASPARINI E, PUCCIARMATI S, PERENZIN M, NOTARIO T, GALLO G, BASILI O, PICCIONI C., AMATO, Gaetano, FRENDA, Alfonso Salvatore, GIAMBALVO, Dario, MOTTA, Valentina, QUARANTA F, BELOCCHI A, DEGIDIO MG, FORNARA M, MAZZON V, DESIDERIO E, BOTTAZZI P, SILVERI D, GALANTE V, MAZZIERI G, PIAZZA C, REGGIANI R, BELLONI P, ANTICHI D, PETRINI A, FUSELLI D, LOMBARDO M G, RACITI CN, DOUST MA, VIRGILLITO S, AMATO G, FRENDA A S, GIAMBALVO D, DE FRANCHI AS, LANDI G, GUIDUCCI M, BOLDRINI A, SANTILOCCHI R, BIANCHELLI M, CAMPI P, SPINA A, CAMBREA M, CODIANNI P, GASPARINI E, PUCCIARMATI S, PERENZIN M, NOTARIO T, GALLO G, MOTTA V, BASILI O, and PICCIONI C

Published
2005

16. Prevention and Treatment of White Spots in Fixed Orthodontics

Author

Lucchese, Alessandra, Manuelli, M., Guiducci, M., Baldoni, E., Trombelli, L., ITALIAN ORTHODONTIC SOCIETY, Lucchese, Alessandra, M., Manuelli, M., Guiducci, E., Baldoni, and L., Trombelli

Subjects
Treatment, Fixed Orthodontics, White Spots Lesions, Prevention
Abstract

Prevention and Treatment of White Spots in Fixed Orthodontics. Aim of Study: To outline a protocol that can reduce or prevent white spots in patients with fixed orthodontics. Material and methods: A total number of 150 patients were selected. White spots were diagnosed in 32 patients (Test Group) during fixed orthodontics. The Test Group was divided into two: Group A (16 patients) and Group B (16 patients). Group A was treated with mouthwash with 0.12% clorexidine diluted to 50% to be applied in the morning for a month as well as a fluoride gel to be applied in the evening for a month. Group B was treated with placebo. Results: In 85% of group A patients there was a statistically significant reduction (p

Published
2008

36. Effetto di volumi irrigui crescenti sulla produzione di un ibrido di mais da granella in semina estiva in diverse località del Centro-Sud

Author

QUAGLIETTA CHIARANDA', FABRIZIO, BARBIERI, GIANCARLO, M. MONOTTI, MONTEMURRO P., SPANU A., VENEZIAN SCARASCIA M. E., CUOCOLO L., GUIDUCCI M., LO SAVIO N., MARTORELLA A., PRUNEDDU G., QUAGLIETTA CHIARANDA', Fabrizio, Barbieri, Giancarlo, M., Monotti, Montemurro, P., Spanu, A., VENEZIAN SCARASCIA, M. E., Cuocolo, L., Guiducci, M., LO SAVIO, N., Martorella, A., and Pruneddu, G.

Subjects
produzione, mai, irrigazione
Published
1982

37. Potenzialità produttiva in semina estiva di mais e sorghi da granella di differente precocità in ambienti dell'Italia centrale e meridionale

Author

CUOCOLO L., RIZZO V., BARBIERI, GIANCARLO, DI BARI V., MARIANI G., MONOTTI M., MONTEMURRO P., SPANU A., BOSCO M., DONATELLI M., GUIDUCCI M., MARTORELLA A., PRUNEDDU G., QUAGLIETTA CHIARANDA', FABRIZIO, Cuocolo, L., Rizzo, V., Barbieri, Giancarlo, DI BARI, V., Mariani, G., Monotti, M., Montemurro, P., QUAGLIETTA CHIARANDA', Fabrizio, Spanu, A., Bosco, M., Donatelli, M., Guiducci, M., Martorella, A., and Pruneddu, G.

Subjects
semina estiva, produzione, Sorgo, Mai
Published
1982

42. Operational experience with DAPHNE beam diagnostics

Author

Serio, M., Bassetti, M., Biagini, M., Biscari, C., Boni, R., Boscolo, M., Chen, S., Chimenti, V., Clozza, A., Delle Monache, G., Simone, S., Di Pirro, G., Drago, A., Faini, S., Gallo, A., Ghigo, A., Guiducci, M., He, Y., Huang, R., Marcellini, F., Marchetti, C., Masullo, M., Mazzitelli, G., Mauro Migliorati, Milardi, C., Modena, M., Palumbo, L., Pellegrino, L., Preger, M., Raffone, M., Ricci, R., Sanelli, C., Sannibale, F., Sgamma, F., Spataro, B., Stecchi, A., Stella, A., Vaccarezza, C., Vescovi, M., Vignola, G., and Zobov, M.

43. First Italian Ebola virus disease case: Management of hospital internal and external communication

Author

Salce, L., Barbato, S., Renna, D., Bianchini, F., Vaccaro, P., Mazzeo, F., Gasparini, A., Rizza, C., Lanfranchi, E., Petrosillo, N., Nicastri, E., Di Caro, A., Capobianchi, M. R., vincenzo puro, Ippolito, G., Bevilacqua, N., Boumis, E., Cicalini, S., Chinello, P., Corpolongo, A., Galati, V., Mariano, A., Rosati, S., Taglietti, F., Vincenzi, L., Antonini, M., Caravella, I., Garotto, G., Marchioni, L., Maritti, M., Biava, G., Rizzi, E. B., Castilletti, C., Bordi, L., Lalle, E., Meschi, S., Lapa, D., Marsella, P., Colavita, F., Chiappini, R., Mazzarelli, A., Quartu, S., Agrati, C., Carletti, F., Forbici, F., Valli, M. B., Abbate, I., Amendola, A., Garbuglia, A. R., Paglia, M. G., Bordi, E., Travaglini, D., Toffoletti, A., Battisti, G., Coppola, A., Marchis, L., Marco, N., Giacomini, P., Di Gianbattista, F., Guiducci, M., Marasco, A., Marzolini, A., Mercuri, A., Nieddu, P., Ondedei, S., Vescovo, M., Vitolo, L., Morea, M., Liguori, M., Lauria, F. N., Russo, A., D Aprile, P., Petrecchia, A., Gentile, M., Pittalis, S., Martini, L., Fusco, F. M., Lanini, S., Antinori, A., and Alberti, V. F.

47. Fate of selenium in soil: A case study in a maize (Zea mays L.) field under two irrigation regimes and fertilized with sodium selenite

Author

Roberto D’Amato, Daniela Businelli, Marcello Guiducci, M. De Feudis, De Feudis, M., D'Amato, R., Businelli, D., and Guiducci, M.

Subjects
Available Se, Irrigation, Selenium fractionation, Leachates, Selenium volatilization, Available Se, Irrigation, Environmental Engineering, 010504 meteorology & atmospheric sciences, chemistry.chemical_element, Growing season, Fractionation, 010501 environmental sciences, Zea mays, complex mixtures, 01 natural sciences, Selenium, Soil, Sodium Selenite, Animal science, Human fertilization, Environmental Chemistry, Fertilizers, Selenium fractionation, Waste Management and Disposal, 0105 earth and related environmental sciences, Volatilisation, Leachate, Trace element, Water, Leachates, Pollution, Selenium volatilization, chemistry, Soil water
Abstract

Selenium (Se) is a trace element necessary for both human and livestock nutrition. To increase Se human intake, soil Se fertilizations were performed but the fate of the added Se remains unclear. The present research aims to: (1) determine the influence of Se fertilization on the fractionation of Se in soil; (2) assess the influence of water availability on the distribution of soil Se chemical fractions; and (3) monitor the Se content in soil, leachates and plants. To reach these goals, 200 g Se ha−1 was applied to soil as sodium selenite in maize crops under two irrigation regimes, and the Se content in plant, soil chemical fractions and leachates were analyzed. Se application increased the total Se content of the soil, specifically it increased the Se content of the soluble, exchangeable and organic fractions with more pronounced effect in the soils with higher water availability. These differences disappeared over time likely due to the Se loss through volatilization. The hypothesis of Se volatilization is confirmed by the absence of both leachates during the maize growing season and differences among the treatments of Se content in sub-soil samples. Also, although the Se treated plants showed higher Se content than the untreated ones, overall 1% of the added Se was assimilated by plants. Hence, this study demonstrated that the addition of selenite to the soil increased the Se contents of the plants, but the Se does not accumulate in the soil because it is likely lost via volatilization. Further, leaching of Se into groundwater is avoided due to its association with both the soil organic matter and positively charged binding sites of soil, and due to its loss via volatilization. Therefore, soil Se fertilization could increase the nutritional value of plants without consequences on the environment.

Published
2019

48. Grain Endogenous Selenium and Moderate Salt Stress Work as Synergic Elicitors in the Enrichment of Bioactive Compounds in Maize Sprouts

Author

Daniela Businelli, Elisabetta Troni, Paolo Benincasa, Gianfranco Diretto, Gian Maria Beone, Roberto D’Amato, Marcello Guiducci, Beatrice Falcinelli, Maria Chiara Fontanella, Sarah Frusciante, Benincasa, P., D'Amato, R., Falcinelli, B., Troni, E., Fontanella, M. C., Frusciante, S., Guiducci, M., Beone, G. M., Businelli, D., and Diretto, G.

Subjects
0106 biological sciences, elicitation, abiotic stress, metabolomics, phytochemical, antioxidant, Antioxidant, abiotic stress, antioxidant, medicine.medical_treatment, chemistry.chemical_element, Salt (chemistry), phytochemical, 01 natural sciences, lcsh:Agriculture, 03 medical and health sciences, Settore AGR/13 - CHIMICA AGRARIA, medicine, Food science, 030304 developmental biology, chemistry.chemical_classification, elicitation, 0303 health sciences, lcsh:S, food and beverages, metabolomics, Salinity, chemistry, Phytochemical, Shoot, Composition (visual arts), Agronomy and Crop Science, Selenium, 010606 plant biology & botany, Sprouting
Abstract

Salt stress and selenium are known to elicitate the production of plant secondary metabolites with antioxidant properties. On this basis, maize grains obtained from mother plants fertilized or not fertilized with selenium were sprouted at different levels of salinity (0, 25, and 50 mM NaCl) to evaluate the effects on the sprout yield, inorganic and organic Se species, minerals, and secondary metabolites, as revealed by a metabolomics analysis. Grain endogenous selenium (135 mg kg-1 vs. 0.19 mg kg-1 of the non-enriched grain) and salinity affected the sprout yield and composition, with salinity having the greatest effect on secondary metabolites. Most of the Se in sprouts was in an inorganic form, despite Se-enriched grains only containing organic Se. Some synergic effect was observed between Se and salinity. The best combination was obtained with Se-enriched grains sprouted at 25 mM NaCl, since this provided a good yield (not lower than in the untreated control), while sprout shoots were enriched in selenocystine and pro-nutritional semipolar compounds with antioxidant properties. Therefore, using grains from Se-fertilized crops and sprouting them under mild salt stress might represent a promising technique for improving the nutritional value of sprouts.

Published
2020

49. Use of MSAP Markers to Analyse the Effects of Salt Stress on DNA Methylation in Rapeseed (Brassica napus var. oleifera)

Author

Marcello Guiducci, Paolo Benincasa, Roberta Pace, Alessandra Traini, Maria Luisa Chiusano, Stanley Lutts, Mario Falcinelli, Lorenzo Raggi, Gianpiero Marconi, Emidio Albertini, Marconi, G, Pace, R, Traini, A, Raggi, L, Lutts, S, Chiusano, MARIA LUISA, Guiducci, M, Falcinelli, M, Benincasa, P, Albertini, E., and UCL - SST/ELI/ELIA - Agronomy

Subjects
Salinity, Genotype, Science, Bisulfite sequencing, rapeseed, Sodium Chloride, Biology, Plant Roots, Gene Expression Regulation, Plant, Stress, Physiological, Epigenetics, Gene, salt stress, Genetics, Regulation of gene expression, MSAP, Epigenetics, DNA methylation, rapeseed, salt stress, Polymorphism, Genetic, Multidisciplinary, DNA methylation, Brassica napus, Brassica rapa, MSAP, Salt Tolerance, Methylation, Plant Leaves, Genetic marker, Medicine, Salts, Genome, Plant, Research Article
Abstract

Excessive soil salinity is a major ecological and agronomical problem, the adverse effects of which are becoming a serious issue in regions where saline water is used for irrigation. Plants can employ regulatory strategies, such as DNA methylation, to enable relatively rapid adaptation to new conditions. In this regard, cytosine methylation might play an integral role in the regulation of gene expression at both the transcriptional and post-transcriptional levels. Rapeseed, which is the most important oilseed crop in Europe, is classified as being tolerant of salinity, although cultivars can vary substantially in their levels of tolerance. In this study, the Methylation Sensitive Amplified Polymorphism (MSAP) approach was used to assess the extent of cytosine methylation under salinity stress in salinity-tolerant (Exagone) and salinity-sensitive (Toccata) rapeseed cultivars. Our data show that salinity affected the level of DNA methylation. In particular methylation decreased in Exagone and increased in Toccata. Nineteen DNA fragments showing polymorphisms related to differences in methylation were sequenced. In particular, two of these were highly similar to genes involved in stress responses (Lacerata and trehalose-6-phosphatase synthase S4) and were chosen to further characterization. Bisulfite sequencing and quantitative RT-PCR analysis of selected MSAP loci showed that cytosine methylation changes under salinity as well as gene expression varied. In particular, our data show that salinity stress influences the expression of the two stress-related genes. Moreover, we quantified the level of trehalose in Exagone shoots and found that it was correlated to TPS4 expression and, therefore, to DNA methylation. In conclusion, we found that salinity could induce genome-wide changes in DNA methylation status, and that these changes, when averaged across different genotypes and developmental stages, accounted for 16.8% of the total site-specific methylation differences in the rapeseed genome, as detected by MSAP analysis. © 2013 Marconi et al.

Published
2013

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