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3. Curcumin and NCLX inhibitors share anti-tumoral mechanisms in microsatellite-instability-driven colorectal cancer

Author

Guéguinou, Maxime, Ibrahim, Sajida, Bourgeais, Jérôme, Robert, Alison, Pathak, Trayambak, Zhang, Xuexin, Crottès, David, Dupuy, Jacques, Ternant, David, Monbet, Valérie, Guibon, Roseline, Flores-Romero, Hector, Lefèvre, Antoine, Lerondel, Stéphanie, Le Pape, Alain, Dumas, Jean-François, Frank, Philippe G., Girault, Alban, Chautard, Romain, Guéraud, Françoise, García-Sáez, Ana J., Ouaissi, Mehdi, Emond, Patrick, Sire, Olivier, Hérault, Olivier, Fromont-Hankard, Gaëlle, Vandier, Christophe, Tougeron, David, Trebak, Mohamed, Raoul, William, and Lecomte, Thierry

Published
2022
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6. Fatty acid profile in peri-prostatic adipose tissue and prostate cancer aggressiveness in African–Caribbean and Caucasian patients

Author

Figiel, Sandy, Pinault, Michelle, Domingo, Isabelle, Guimaraes, Cyrille, Guibon, Roseline, Besson, Pierre, Tavernier, Elsa, Blanchet, Pascal, Multigner, Luc, Bruyère, Franck, Haillot, Olivier, Mathieu, Romain, Vincendeau, Sebastien, Rioux-Leclercq, Nathalie, Lebdai, Souhil, Azzouzi, Abdel-Rahmene, Perrouin-Verbe, Marie-Aimee, Fournier, Georges, Doucet, Laurent, Rigaud, Jerome, Renaudin, Karine, Mahéo, Karine, and Fromont, Gaëlle

Published
2018
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8. Curcumin and NCLX Inhibitors Share Anti-Tumoral Mechanisms in Microsatellite-Instability-Driven Colorectal Cancer

Author

Guéguinou, Maxime, primary, Ibrahim, Sajida, additional, Bourgeais, Jérôme, additional, Robert, Alison, additional, Pathak, Trayambak, additional, Zhang, Xuexin, additional, Crottès, David, additional, Dupuy, Jacques, additional, Ternant, David, additional, Monbet, Valérie, additional, Guibon, Roseline, additional, Flores-Romero, Hector, additional, Lefèvre, Antoine, additional, Lerondel, Stéphanie, additional, Le Pape, Alain, additional, Dumas, Jean-François, additional, Frank, Philippe G., additional, Girault, Alban, additional, Chautard, Romain, additional, Guéraud, Françoise, additional, García-Sáez, Ana J., additional, Ouaissi, Mehdi, additional, Emond, Patrick, additional, Sire, Olivier, additional, Hérault, Olivier, additional, Fromont-Hankard, Gaëlle, additional, Vandier, Christophe, additional, Tougeron, David, additional, Trebak, Mohamed, additional, Raoul, William, additional, and Lecomte, Thierry, additional

Published
2022
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9. Tissue cholesterol metabolism and prostate cancer aggressiveness: Ethno‐geographic variations

Author

Lethongsavarn, Vincent, primary, Pinault, Michèle, additional, Diedhiou, Abdoulaye, additional, Guimaraes, Cyrille, additional, Guibon, Roseline, additional, Bruyère, Franck, additional, Mathieu, Romain, additional, Rioux‐Leclercq, Nathalie, additional, Multigner, Luc, additional, Brureau, Laurent, additional, Fournier, Georges, additional, Doucet, Laurent, additional, Blanchet, P., additional, and Fromont, Gaëlle, additional

Published
2021
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12. The Calcium-Sensing Receptor is A Marker and Potential Driver of Neuroendocrine Differentiation in Prostate Cancer

Author

Bery, Fanny, Cancel, Mathilde, Chantôme, Aurélie, Guibon, Roseline, Bruyère, Franck, Rozet, François, Mahéo, Karine, and Fromont, Gaëlle

Subjects
calcium, hormone therapy, calcium-sensing receptor, prostate cancer, neuroendocrine differentiation, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282, Article
Abstract

The mechanisms underlying neuroendocrine (NE) differentiation in prostate cancer (PCa) remain mostly uncharacterized. Since a deregulated calcium homeostasis has been reported in neuroendocrine prostate cancer (NEPC), we explored herein the link between NE differentiation and the calcium-sensing receptor (CaSR). CaSR expression was evaluated by immunohistochemistry&mdash, together with NE markers&mdash, on tissue microarrays containing samples of normal prostate, localized PCa, metastatic castration resistant PCa (MCRPC) and NEPC. In prostate tissues, we observed a strong association between CaSR and chromogranin expression. Both markers were strongly expressed in all cases of NEPC and co-expression was confirmed by double immunostaining. In MCRPC, the expression of CaSR was significantly associated with shorter overall survival. The involvement of CaSR in NE differentiation was evaluated in PCa cell lines. Inhibition of CaSR led to decrease the expression of neuronal (NSE, &beta, tubulinIII) and NE (chromogranin, synaptophysin) markers in the NE PCa cell line NCI-H660. A decrease of neuronal and NE markers was also observed in siCaSR-transfected PC3 and 22RV1 cells, respectively, whereas CaSR activation increased both NSE and synaptophysin expression in PC3 cells. These results strongly suggest that CaSR is a marker and a driver of NE differentiation in PCa and emphasize the potential of CaSR directed therapy for NEPC patients.

Published
2020

13. A comparative study of the capacity of mesenchymal stromal cell lines to form spheroids

Author

Deynoux, Margaux, Sunter, Nicola, Ducrocq, Elfi, Dakik, Hassan, Guibon, Roseline, Burlaud-Gaillard, Julien, Brisson, Lucie, Rouleux-Bonnin, Florence, le Nail, Louis-Romée, Hérault, Olivier, Domenech, Jorge, Roingeard, Philippe, Fromont, Gaëlle, Mazurier, Frédéric, ERL 7001 LNOx (Leukemic Niche & redOx metabolism / Niche leucémique et métabolisme redOx) (LNOx), Groupe innovation et ciblage cellulaire (GICC), EA 7501 [2018-...] (GICC EA 7501), Université de Tours (UT)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Anatomie et cytologie pathologique [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Nutrition, croissance et cancer (U 1069) (N2C), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Plateforme IBISA de Microscopie Electronique [CHRU de Tours] (UNIV Tours), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Université de Tours (UT), Morphogénèse et antigénicité du VIH et du virus des Hépatites (MAVIVH - U1259 Inserm - CHRU Tours ), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Chirurgie orthopédique [Tours], Service d'Hématologie biologique [Tours], The authors acknowledge the Ministry of Research (MD), the ARC Foundation (MD, HD), the French Society of Hematology (MD), the 'Ligue contre le Cancer (NS)', and the Lebanese south governate (HD) for their funding. This work was supported by the French Committees of the 'Ligue Contre le Cancer Grand-Ouest' [16 (Charente), 36 (Indre), 37 (Indre-et-Loire), 41 (Loire et Cher), and 86 (Vendée)] and the Région Centre Val de Loire (FM)., Bodescot, Myriam, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Centre National de la Recherche Scientifique (CNRS)-Microenvironnement des niches tumorales (CNRS GDR 3697 Micronit ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Groupe innovation et ciblage cellulaire (GICC), EA 7501 [2018-...] (GICC EA 7501), Université de Tours (UT)-Université de Tours (UT), Université de Tours-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)-Université de Tours, and Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)

Subjects
Autophagic Cell Death, Cell Lines, Science, Apoptosis, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Research and Analysis Methods, Cell Line, Mice, Animal Cells, Spheroids, Cellular, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Animals, Humans, Electron Microscopy, Hypoxia, Cell Aggregation, Cell Proliferation, Staining, Microscopy, Cell Death, Stem Cells, Biology and Life Sciences, Cell Staining, Mesenchymal Stem Cells, Cell Biology, Cell Dedifferentiation, Cell Hypoxia, Oxidative Stress, Cell Processes, Specimen Preparation and Treatment, embryonic structures, Medicine, Biological Cultures, Scanning Electron Microscopy, Cellular Types, Stem Cell Lines, Research Article
Abstract

International audience; Mesenchymal stem cells (MSC)-spheroid models favor maintenance of stemness, ex vivo expansion and transplantation efficacy. Spheroids may also be considered as useful surrogate models of the hematopoietic niche. However, accessibility to primary cells, from bone marrow (BM) or adipose tissues, may limit their experimental use and the lack of consistency in methods to form spheroids may affect data interpretation. In this study, we aimed to create a simple model by examining the ability of cell lines, from human (HS-27a and HS-5) and murine (MS-5) BM origins, to form spheroids, compared to primary human MSCs (hMSCs). Our protocol efficiently allowed the spheroid formation from all cell types within 24 hours. Whilst hMSC-spheroids began to shrink after 24 hours, the size of spheroids from cell lines remained constant during three weeks. The difference was partially explained by the balance between proliferation and cell death, which could be triggered by hypoxia and induced oxidative stress. Our results demonstrate that, like hMSCs, MSC cell lines make reproductible spheroids that are easily handled. Thus, this model could help in understanding mechanisms involved in MSC functions and may provide a simple model by which to study cell interactions in the BM niche.

Published
2020

14. Additional file 2 of Apolipoprotein-mediated regulation of lipid metabolism induces distinctive effects in different types of breast cancer cells

Author

Hassen, Céline Ben, Gutierrez-Pajares, Jorge L., Guimaraes, Cyrille, Guibon, Roseline, Pinault, Michelle, Fromont, Gaëlle, and Frank, Philippe G.

Abstract

Additional file 2:Supplementary Figure 2. ApoA-I and apoE increase proliferation of MCF-7 (A) but not of MDA-MB-231 (B) cells as determined by cell counting measurements. ns: difference with control not significant. Significant difference compared to control cells: *P

Published
2020
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16. A Novel Calcium-Mediated EMT Pathway Controlled by Lipids: An Opportunity for Prostate Cancer Adjuvant Therapy

Author

Figiel, Sandy, Bery, Fanny, Chantôme, Aurélie, Fontaine, Delphine, Pasqualin, Côme, Maupoil, Véronique, Domingo, Isabelle, Guibon, Roseline, Bruyere, Franck, Potier-Cartereau, Marie, Vandier, Christophe, Fromont, Gaëlle, Mahéo, Karine, Nutrition, croissance et cancer (U 1069) (N2C), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Service d'urologie [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Hôpital Bretonneau, Service d'Anatomie et de Cytologie Pathologiques [Poitiers], Centre hospitalier universitaire de Poitiers (CHU Poitiers), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers, Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Hôpital Bretonneau-Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)

Subjects
linoleic acid, eicosapentaenoic acid, calcium, [SDV.CAN]Life Sciences [q-bio]/Cancer, zeb1, prostate cancer, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282, Article, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

The composition of periprostatic adipose tissue (PPAT) has been shown to play a role in prostate cancer (PCa) progression. We recently reported an inverse association between PCa aggressiveness and elevated PPAT linoleic acid (LA) and eicosapentaenoic acid (EPA) content. In the present study, we identified a new signaling pathway with a positive feedback loop between the epithelial-to-mesenchymal transition (EMT) transcription factor Zeb1 and the Ca2+-activated K+ channel SK3, which leads to an amplification of Ca2+ entry and cellular migration. Using in vitro experiments and ex vivo cultures of human PCa slices, we demonstrated that LA and EPA exert anticancer effects, by modulating Ca2+ entry, which was involved in Zeb1 regulation and cancer cellular migration. This functional approach using human prostate tumors highlights the clinical relevance of our observations, and may allow us to consider the possibility of targeting cancer spread by altering the lipid microenvironment.

Published
2019

18. Analyse IRM de thrombi impliqués dans l’AVC ischemique

Author

Janot, Kevin, primary, Oliveira, Tiago Ribeiro, additional, Herbreteau, Denis, additional, Bibi, Richard, additional, Ifergan, Héloïse, additional, Maldonado, Igor, additional, Barrot, Valère, additional, Fromont-hankard, Gaëlle, additional, Annan, Mariam, additional, Filipiak, Isabelle, additional, Barantin, Laurent, additional, Guibon, Roseline, additional, Cottier, Jean-Philippe, additional, and Narata, Ana Paula, additional

Published
2020
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19. A comparative study of the capacity of mesenchymal stromal cell lines to form spheroids

Author

Deynoux, Margaux, primary, Sunter, Nicola, additional, Ducrocq, Elfi, additional, Dakik, Hassan, additional, Guibon, Roseline, additional, Burlaud-Gaillard, Julien, additional, Brisson, Lucie, additional, le Nail, Louis-Romée, additional, Hérault, Olivier, additional, Domenech, Jorge, additional, Roingeard, Philippe, additional, Fromont, Gaëlle, additional, and Mazurier, Frédéric, additional

Published
2019
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21. Quantitative estimation of thrombus-erythrocytes using MRI. A phantom study with clot analogs and analysis by statistic regression models

Author

Janot, Kevin, primary, Oliveira, Tiago Ribeiro, additional, Fromont-Hankard, Gaelle, additional, Annan, Mariam, additional, Filipiak, Isabelle, additional, Barantin, Laurent, additional, Guibon, Roseline, additional, Duffy, Sharon, additional, Gilvarry, Michael, additional, Cottier, Jean-Philippe, additional, and Narata, Ana Paula, additional

Published
2019
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22. Impact du remodelage de la signalisation calcique dans la selection des patients éligibles à la surveillance active dans le cancer de prostate de faible risque

Author

Schoentgen, Nadja, Perrouin-Verbe, Marie-Aimée, Guibourg, Briac, Uguen, Arnaud, Buscaglia, Paul, Guibon, Roseline, L'hostis, Edith, Doucet, Laurent, Marcorelles, Pascale, Pers, Jacques-Olivier, Fromont, Gaëlle, Fournier, Georges, Valéri, Antoine, Mignen, Olivier, Lymphocyte B et Auto-immunité (LBAI), Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Chirurgie Urologique (BREST - Urologie), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), CHRU Brest - Laboratoire d'Anatomo-Pathologie (CHU - AnaPath), Nutrition, croissance et cancer (U 1069) (N2C), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Neurosciences de Brest (LNB), Université de Brest (UBO), Service d'Anatomopathologie [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Centre de Recherche pour les Pathologies Prostatiques [Paris] (CeRePP), Sorbonne Université (SU), LabEX IGO Immunothérapie Grand Ouest, Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Brestois Santé Agro Matière (IBSAM), and Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
[SDV.IMM]Life Sciences [q-bio]/Immunology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

23. Quantitative estimation of thrombus-erythrocytes using MRI. A phantom study with clot analogs and analysis by statistic regression models.

Author

Janot, Kevin, Oliveira, Tiago Ribeiro, Fromont-Hankard, Gaelle, Annan, Mariam, Filipiak, Isabelle, Barantin, Laurent, Guibon, Roseline, Duffy, Sharon, Gilvarry, Michael, Cottier, Jean-Philippe, and Narata, Ana Paula

Subjects
ERYTHROCYTES, MAGNETIC resonance imaging, IMAGING phantoms, REGRESSION analysis, STROKE, THROMBOSIS, VEIN surgery, MULTIPLE regression analysis, IN vitro studies
Abstract

Background Thrombus composition has the potential to affect acute ischemic stroke (AIS) treatment. Objective To evaluate in an in vitro test the correlation of clot composition, especially erythrocytes (red blood cells (RBGs)), with the variation of signal intensity ratio (SIR) obtained with MRI sequences used for AIS, and qualification of the susceptibility vessel sign effect using clot analogs. Materials and methods Nine ovine clots were fixed in a gelatin-manganese solution and studied by MRI (T2GE, T2-weighted gradient echo; SWI, susceptibility-weighted imaging; FLAIR, fluid attenuated inversion recovery). RBG concentration was estimated using regression models (5LR, single linear regression; MLR, multiple linear regression; RF, random Forest; and ANN, artificial neural networking), which combined the SIR-histology relationship of three MRI sequences. results Negative correlation was found between SIR and RBC concentration. T2GE SWI could not statistically distinguish clots with RBC content >54% and <23%. SLR was applied only to FLAIR images since T2GE and SWI demonstrated signal saturation. All four regression models showed a correlation between MRI and histology: SLR=0.981; MLR=0.986; RF=0.994, and ANN=0.971. One unknown clot was studied and agreement between SIR and histological analyses was found in all models. Conclusions We presented a method to quantify RBC concentration in clot analogs, combining SWI, T2GE, and FLAIR. This in vitro study has some limitations, so clot collection after thrombectomy with simultaneous imaging analysis is necessary to validate this model. [ABSTRACT FROM AUTHOR]

Published
2020
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24. 4TM-TRPM8 channels are new gatekeepers of the ER-mitochondria Ca2+ transfer

Author

Bidaux, Gabriel, primary, Gordienko, Dmitri, additional, Shapovalov, George, additional, Farfariello, Valerio, additional, Borowiec, Anne-sophie, additional, Iamshanova, Oksana, additional, Lemonnier, Loic, additional, Gueguinou, Maxime, additional, Guibon, Roseline, additional, Fromont, Gaelle, additional, Paillard, Mélanie, additional, Gouriou, Yves, additional, Chouabe, Christophe, additional, Dewailly, Etienne, additional, Gkika, Dimitra, additional, López-Alvarado, Pilar, additional, Carlos Menéndez, J., additional, Héliot, Laurent, additional, Slomianny, Christian, additional, and Prevarskaya, Natalia, additional

Published
2018
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26. Carcinoma-specific expression of P2Y11 receptor and its contribution in ATP-induced purinergic signalling and cell migration in human hepatocellular carcinoma cells

Author

Khalid, Madiha, primary, Brisson, Lucie, additional, Tariq, Menahil, additional, Hao, Yunjie, additional, Guibon, Roseline, additional, Fromont, Gaëlle, additional, Syed Mortadza, Sharifah Alawieyah, additional, Mousawi, Fatema, additional, Manzoor, Sobia, additional, Roger, Sébastien, additional, and Jiang, Lin-Hua, additional

Published
2017
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28. Polymorphism in programmed cell death 1 gene is strongly associated with lung and kidney allograft survival in recipients from CMV-positive donors

Author

Forconi, Catherine, primary, Gatault, Philippe, additional, Miquelestorena-Standley, Elodie, additional, Noble, Johan, additional, Al-Hajj, Sally, additional, Guillemain, Romain, additional, Stern, Marc, additional, Hoffmann, Thomas, additional, Prat, Louis, additional, Suberbielle, Caroline, additional, Masson, Emeline, additional, Cesbron-Gautier, Anne, additional, Gaudy-Graffin, Catherine, additional, Goudeau, Alain, additional, Thibault, Gilles, additional, Ivanes, Fabrice, additional, Guibon, Roseline, additional, Kazma, Ihab, additional, Lebranchu, Yvon, additional, Büchler, Matthias, additional, Magnan, Antoine, additional, Halimi, Jean-Michel, additional, and Baron, Christophe, additional

Published
2017
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29. SCN4B acts as a metastasis-suppressor gene preventing hyperactivation of cell migration in breast cancer

Author

Bon, Emeline, primary, Driffort, Virginie, additional, Gradek, Frédéric, additional, Martinez-Caceres, Carlos, additional, Anchelin, Monique, additional, Pelegrin, Pablo, additional, Cayuela, Maria-Luisa, additional, Marionneau-Lambot, Séverine, additional, Oullier, Thibauld, additional, Guibon, Roseline, additional, Fromont, Gaëlle, additional, Gutierrez-Pajares, Jorge L., additional, Domingo, Isabelle, additional, Piver, Eric, additional, Moreau, Alain, additional, Burlaud-Gaillard, Julien, additional, Frank, Philippe G., additional, Chevalier, Stéphan, additional, Besson, Pierre, additional, and Roger, Sébastien, additional

Published
2016
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30. Functional Organotypic Cultures of Prostate Tissues

Author

Figiel, Sandy, Pasqualin, Côme, Bery, Fanny, Maupoil, Veronique, Vandier, Christophe, Potier-Cartereau, Marie, Domingo, Isabelle, Guibon, Roseline, Bruyere, Franck, Maheo, Karine, and Fromont, Gaelle

Abstract

In prostate cancer research, there is a lack of valuable preclinical models. Tumor cell heterogeneity and sensitivity to microenvironment signals, such as hypoxia or extracellular calcium concentration, are difficult to reproduce. Here, we developed and characterized an ex vivotissue culture model preserving these properties. Prostate tissue slices from 26 patients were maintained ex vivounder optimized culture conditions. The expression of markers associated with proliferation, androgen-receptor signaling, and hypoxia was assessed by immunostaining. A Macro Zoom System Microscope was used to achieve real-time calcium fluorescence optical imaging. Tissue morphology was maintained successfully without necrosis for 5 days. Compared with native tumors and tissue cultured with androgens, androgen deprivation in the medium led to decreased expression of both androgen receptor and its target gene products, PSA and ERG. Ex vivocultured slices also were sensitive to hypoxia because carbonic anhydrase IX and Zeb1 protein levels increased in 1% oxygen. Exposure of slices to supraphysiological extracellular Ca2+concentration induced a robust and rapid Ca2+entry, with a greater response in tumor compared with nontumor tissue. This ex vivomodel reproduces the morphologic and functional characteristics of human prostate cancer, including sensitivity to androgen deprivation and induced response to hypoxia and extracellular Ca2+. It therefore could become an attractive tool for drug response prediction studies.

Published
2024
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31. 0408 : Stimulation of P2Y11 purinergic receptor reduces rejection lesions and increases survival time of heart allograft in a mouse model

Author

Bourguignon, Thierry, primary, Klipfel-Lhommet, Clémence, additional, Ivanes, Fabrice, additional, Chadet, Stéphanie, additional, Benoist, Lauriane, additional, Lefort, Claudie, additional, Guibon, Roseline, additional, Fromont, Gaelle, additional, Jayle, Christophe, additional, Aupart, Michel, additional, and Angoulvant, Denis, additional

Published
2016
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34. Towards the Establishment of a Porcine Model to Study Human Amebiasis

Author

Girard-Misguich, Fabienne, primary, Cognie, Juliette, additional, Delgado-Ortega, Mario, additional, Berthon, Patricia, additional, Rossignol, Christelle, additional, Larcher, Thibaut, additional, Melo, Sandrine, additional, Bruel, Timothée, additional, Guibon, Roseline, additional, Chérel, Yan, additional, Sarradin, Pierre, additional, Salmon, Henri, additional, Guillén, Nancy, additional, and Meurens, François, additional

Published
2011
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35. 4TM-TRPM8 channels are new gatekeepers of the ER-mitochondria Ca 2+ transfer.

Author

Bidaux G, Gordienko D, Shapovalov G, Farfariello V, Borowiec AS, Iamshanova O, Lemonnier L, Gueguinou M, Guibon R, Fromont G, Paillard M, Gouriou Y, Chouabe C, Dewailly E, Gkika D, López-Alvarado P, Carlos Menéndez J, Héliot L, Slomianny C, and Prevarskaya N

Subjects
Aged, Alternative Splicing, Cell Line, Tumor, Epithelial Cells cytology, Epithelial Cells metabolism, Gene Expression Regulation, Neoplastic, Humans, Male, Middle Aged, Prostate cytology, Prostate metabolism, Prostatic Neoplasms genetics, Protein Domains, TRPM Cation Channels chemistry, Calcium metabolism, Endoplasmic Reticulum metabolism, Mitochondria metabolism, Prostatic Neoplasms metabolism, TRPM Cation Channels genetics, TRPM Cation Channels metabolism
Abstract

Calcium (Ca 2+ ) release from the endoplasmic reticulum plays an important role in many cell-fate defining cellular processes. Traditionally, this Ca 2+ release was associated with the ER Ca 2+ release channels, inositol 1,4,5‑triphosphate receptor (IP 3 R) and ryanodine receptor (RyR). Lately, however, other calcium conductances have been found to be intracellularly localized and to participate in cell fate regulation. Nonetheless, molecular identity and functional properties of the ER Ca 2+ release mechanisms associated with multiple diseases, e.g. prostate cancer, remain unknown. Here we identify a new family of transient receptor potential melastatine 8 (TRPM8) channel isoforms as functional ER Ca 2+ release channels expressed in mitochondria-associated ER membranes (MAMs). These TRPM8 isoforms exhibit an unconventional structure with 4 transmembrane domains (TMs) instead of 6 TMs characteristic of the TRP channel archetype. We show that these 4TM-TRPM8 isoforms form functional channels in the ER and participate in regulation of the steady-state Ca 2+ concentration ([Ca 2+ ]) in mitochondria and the ER. Thus, our study identifies 4TM-TRPM8 isoforms as ER Ca 2+ release mechanism distinct from classical Ca 2+ release channels., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Published
2018
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