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TO explore the changes of rhizosphere soil bacterial community of Rosa rugosa "Fenghua", Rosa rugosa cv. Plena and Rosa rugosa "Zizhi" in different seasons, the Illumina Miseq sequencing and the correlation network analysis of dominant flora was used. The results showed that the bacterial communities were mainly composed of Proteobacteria, Acidobacteria, Bacteroidetes, and Actinobacteria, with Sphingomonas, GP6, GP4, Novosphingobium, Wps-1_genera_incertae_sedis, and Massilia as the dominant genera. The correlation network analysis showed that, as the dominant group with the highest relative abundance, Sphingomonas had a significant positive correlation with Gemmatimonas, Aridibacter, GP3, GP4, and Flavisolibacter, and a significant negative correlation with Solirubrobacter, indicating that it could work synergistically with a variety of microorganisms to contribute to soil metabolism and the growth and development of roses. The results revealed the diversity of microbial structures in the rhizosphere soil of Rosa rugosa "Fenghua", Rosa rugosa cv. Plena and Rosa rugosa "Zizhi", and this will provide a theoretical basis for exploring the change rules of microbial communities, screening and utilizing beneficial microorganisms, and maintaining the growth and development of roses. KEY POINTS: • Variations from season to season significantly affected the bacterial community structure. • There was less variability in the bacterial community structure between rose varieties. • Sphingomonas was the dominant bacterium in all seasons.

Molecular markers are important genetic tools that permit the detection and utilization of genetic variation in germplasm resources. To develop high-density single nucleotide polymorphisms (SNPs) markers for rose, specific length amplified fragment sequencing (SLAF-seq) was performed in 158 rose individuals using the Rosa chinensis Jacq. genome as a reference for enzyme digestion prediction. After high-throughput sequencing, SNP marker development, and evolutionary analysis, the phylogenetic trees of 158 rose individuals were constructed according to the SNPs. A total of 1,703,904 SLAF tags were developed, among which 277,749 were polymorphic SLAF tags. According to the obtained polymorphic SLAF tags, a total of 1,816,980 population SNP markers were obtained, and 17,233 highly consistent SNP markers. The cluster analysis revealed that 158 rose individuals could be grouped into two major clusters. The average observed heterozygosity and polymorphism information content were 0.1802 and 0.2306, respectively. The population structure analysis of 158 rose individuals (K = 12) indicated that most of the populations had different ancestors and showed signs of crossover. The obtained SNPs in the rose genome can aid variety identification and have important implications for the conservation and improvement of varieties.

The purpose of this study was to compare the physicochemical properties and flavor components of rose jam from different regions to evaluate the quality of rose jam. The LC-ESI-MS analysis showed that the cyanidin was the main anthocyanin and quercetin was the main flavonoid in rose jam. The VOCs of rose jam in different regions were analyzed by HS-GC-MS and HS-GC-IMS, and the flavor fingerprint of rose jam was established with principal component analysis. The VOCs content in rose jam was different in different regions, but the VOCs content in the same region was similar. Alcohols, esters and aldehydes were the main flavor substances in rose jam. The HS-GC-IMS technology can be used to distinguish the volatile flavor compounds in rose jam and identify the true and false of Pingyin rose jam, and has high feasibility in the quality evaluation and identification of rose jam. Moreover, the rose jam samples from different regions can be well distinguished by principal component analysis. This study deepened the understanding of the physicochemical properties and VOCs of rose jam, and provided reference for the identification of rose jam in different regions.

To observe the radiotherapy sensitization effect of retroperitoneal lymph node metastasis in patients by sodium glycididazole in recent clinical efficacy and toxicity. A total of 42 patients admitted with metastasis and recurrence of retroperitoneal lymph node from September 2006 to December 2009 were classified with the method of case-control. After three dimensional conformal radiation therapy with or without sodium glycididazole (800 mg/m²) for sensitization, the results of recent clinical efficacy, relief of pain, and Karnofsky score were obtained. Tumor remission rate of patients in sensitization group (with sodium glycididazole) at post-radiotherapy 3 months was significant higher than that in control group (without sodium glycididazole) (52% vs. 24%; P0.05). Oral dose of morphine daily, and Karnofsky score in anterior-posterior radiotherapy of patients in the sensitization group were significant different with those in the control group (93 ± 12 and 42 ± 6 mg vs. 94 ± 12 and 20 ± 5 mg and (65 ± 4) and (90 ± 9) vs. (64 ± 5) and (80 ± 10), respectively; P0.01). Sodium glycididazole has positive radiotherapy sensitization to the metastasis or recurrence of retroperitoneal lymph node for digestive tract cancer, which could obviously improve the life quality or release the pain for patients.

To compare the clinical therapeutic effects between ozone and triamcinolone acetonide for the treatment of mild meniscal injury.From January 2008 to December 2012, 119 patients with meniscal injury diagnosed as type I or II by MRI were divided into three groups. In the triamcinolone acetonide (A) group, there were 38 males and 2 females, with an average age of (25.34 +/- 6.34) years old, ranging from 18 to 48 years old; 36 patients had single knee injuries, 4 patients had double knee injuries and 44 knees with joint effusion. In the ozone (B) group,there were 37 males and 2 females, with an average age of (26.98 +/- 7.20) years old, ranging from 19 to 50 years old; 33 patients had single knee injuries, 6 patiens had double knees injuries and 40 knees with joint effusion. In the combination of ozone and triamcinolone acetonide (C) group, there were 37 males and 3 females,with an average age of (26.44 +/- 6.38) years old, ranging from 18 to 47 years old; 33 patients had single knee injuries, 7 patients had double knees injuries and 39 knees with joint effusion. The patients were treated with injection of 3 mg triamcinolone acetonide alone, 30 ml (30 microg/ml) ozone alone, or both two drugs respectively in knee joint cavity. All the treatment methods were carried out weekly and 4 times injections were required for a treatment course. Knee joint function was evaluated by Lysholm scale and knee joint effusion was examined by MRI before and after treatment.The scale of knee joint function was 35.68 +/- 4.15 and 65.55 +/- 7.66 in group A, 36.35 +/- 6.83 and 74.39 +/- 8.47 in group B, 36.62 +/- 6.03 and 95.47 +/- 4.78 in group C before and after treatment. Compared with that before treatment, the total scale of knee joint function improved after treatment in every group. The total scale of group C was better than that of the other groups after the treatment,but there was no significant difference between group A and group B. The effective rate of these drugson joint effusion was 68.18% in group A, 62.5% in group B and 87.18% in group C. The effect of co-injection on joint effusion in group C was significantly better than that of triamcinolone acetonide or ozone alone, but the difference between group A and group B was not significant.Ozone and triamcinolone acetonide are effective in the treatment of mild meniscal injury, which can relieve symptoms and promote the recovery of joint function. Campared with the single injection, the combination of ozone and triamcinolone acetonide is better.