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2. Electrochemical Growth of Copper Crystals on SPCE for Electrocatalysis Nitrate Reduction

Author

Roberta Farina, Giuseppe D’Arrigo, Alessandra Alberti, Giuseppe E. Capuano, Domenico Corso, Giuseppe A. Screpis, Maria Anna Coniglio, Guglielmo G. Condorelli, and Sebania Libertino

Subjects
copper micro-flowers, electrochemical deposition, cyclic voltammetry, nitrate reduction, Chemistry, QD1-999
Abstract

Copper is efficient, has a high conductivity (5.8 × 107 S/m), and is cost-effective. The use of copper-based catalysts is promising for the electrocatalytic reduction of nitrates. This work aims to grow and characterize copper micro-crystals on Screen-Printed Electrodes (SPEs) for NO3− reduction in water. Copper micro-crystals were grown by cyclic voltammetry. Different cycles (2, 5, 7, 10, 12, 15) of copper electrodeposition were investigated (potential ranges from −1.0 V to 0.0 V, scan rate of 0.1 V s−1). Electrodeposition generated different morphologies of copper crystals on the electrodes, as a function of the number of cycles, with various performances. The presence of numerous edges and defects in the copper micro-crystal structures creates highly reactive active sites, thus favoring nitrate reduction. The manufactured material can be successfully employed for environmental applications.

Published
2024
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4. Copper Micro-Flowers for Electrocatalytic Sensing of Nitrate Ions in Water

Author

Roberta Farina, Giuseppe D’Arrigo, Alessandra Alberti, Silvia Scalese, Giuseppe E. Capuano, Domenico Corso, Giuseppe A. Screpis, Maria Anna Coniglio, Guglielmo G. Condorelli, and Sebania Libertino

Subjects
nitrate detection, electrochemical sensor, screen-printed electrodes, electrocatalytic reaction, electrodeposition, Chemical technology, TP1-1185
Abstract

The progressive increase in nitrate’s (NO3−) presence in surface and groundwater enhances environmental and human health risks. The aim of this work is the fabrication and characterization of sensitive, real-time, low-cost, and portable amperometric sensors for low NO3− concentration detection in water. Copper (Cu) micro-flowers were electrodeposited on top of carbon screen-printed electrodes (SPCEs) via cyclic voltammetry (with voltage ranging from −1.0 V to 0.0 V at a scan rate of 0.1 V s−1). The obtained sensors exhibited a high catalytic activity toward the electro-reduction in NO3−, with a sensitivity of 44.71 μA/mM. They had a limit of detection of 0.87 µM and a good dynamic linear concentration range from 0.05 to 3 mM. The results were compared to spectrophotometric analysis. In addition, the devices exhibited good stability and a maximum standard deviation (RSD) of 5% after ten measurements; reproducibility, with a maximum RSD of 4%; and repeatability after 10 measurements with the RSD at only 5.63%.

Published
2024
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5. Silicon‐Based Sensing Surface for Alzheimer's Disease Diagnosis by Phages Probes

Author

Paolo Calorenni, Vincenzo Paratore, Maria G. Rizzo, Laura M. De Plano, Guglielmo G. Condorelli, Giovanna De Luca, Salvatore Oddo, Salvatore Guglielmino, Emanuele L. Sciuto, and Sabrina Conoci

Subjects
Alzheimer's disease diagnosis, phage display, silicon‐based biointerface, surface morphological and chemical characterization, Physics, QC1-999, Technology
Abstract

Abstract Alzheimer's disease (AD) is a diffused neurodegenerative disorder affecting people in advanced age causing loss of memory and dementia. Nowadays, diagnosis and treatment of AD are still challenging due to the lack of diagnostic systems that allow for an early and reliable diagnosis and therapy monitoring. Moreover, conventional strategies for AD diagnosis are based on brain imaging techniques that are invasive and expensive for early and massive screening. Phage display approach, using engineered phage probe for direct amyloid‐β (Aβ)‐autoantibodies detection, overcome these limitations leading to the possibility of safe and low‐cost screening. Moreover, the combination with silicon technology further improves the easiness of diagnosis due to the portability of devices and the integration of sensitive transduction signals. In this work, an innovative silicon‐based sensing technology is reported detecting Aβ‐autoantibodies, specifically Immunoglobulin G (IgG), in human sera by engineered M13‐phage probes (ADPP). The strategy hinges on a bio‐surface that is integrated on top of a silicon biosensor. Thanks to phages probes exposing Aβ‐mimic peptides, this chip can capture and reveal Aβ‐autoantibodies, discriminating between healthy and AD conditions. The surface chemistry is morphologically and chemically characterized and the phage‐based biosensor ability to recognise Aβ‐autoantibodies is proved by transduction with enzyme‐linked anti‐M13 antibodies.

Published
2024
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7. Electrochemical Growth of Copper Crystals on SPCE for Electrocatalysis Nitrate Reduction.

Author

Farina, Roberta, D'Arrigo, Giuseppe, Alberti, Alessandra, Capuano, Giuseppe E., Corso, Domenico, Screpis, Giuseppe A., Coniglio, Maria Anna, Condorelli, Guglielmo G., and Libertino, Sebania

Subjects
COPPER crystals, COPPER, COPPER electrodes, CYCLIC voltammetry, CRYSTAL morphology, DENITRIFICATION
Abstract

Copper is efficient, has a high conductivity (5.8 × 107 S/m), and is cost-effective. The use of copper-based catalysts is promising for the electrocatalytic reduction of nitrates. This work aims to grow and characterize copper micro-crystals on Screen-Printed Electrodes (SPEs) for NO3− reduction in water. Copper micro-crystals were grown by cyclic voltammetry. Different cycles (2, 5, 7, 10, 12, 15) of copper electrodeposition were investigated (potential ranges from −1.0 V to 0.0 V, scan rate of 0.1 V s−1). Electrodeposition generated different morphologies of copper crystals on the electrodes, as a function of the number of cycles, with various performances. The presence of numerous edges and defects in the copper micro-crystal structures creates highly reactive active sites, thus favoring nitrate reduction. The manufactured material can be successfully employed for environmental applications. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Copper Micro-Flowers for Electrocatalytic Sensing of Nitrate Ions in Water.

Author

Farina, Roberta, D'Arrigo, Giuseppe, Alberti, Alessandra, Scalese, Silvia, Capuano, Giuseppe E., Corso, Domenico, Screpis, Giuseppe A., Coniglio, Maria Anna, Condorelli, Guglielmo G., and Libertino, Sebania

Subjects
AMPEROMETRIC sensors, CARBON electrodes, NITRATES, COPPER, WATER table, ENVIRONMENTAL health
Abstract

The progressive increase in nitrate's (NO3−) presence in surface and groundwater enhances environmental and human health risks. The aim of this work is the fabrication and characterization of sensitive, real-time, low-cost, and portable amperometric sensors for low NO3− concentration detection in water. Copper (Cu) micro-flowers were electrodeposited on top of carbon screen-printed electrodes (SPCEs) via cyclic voltammetry (with voltage ranging from −1.0 V to 0.0 V at a scan rate of 0.1 V s−1). The obtained sensors exhibited a high catalytic activity toward the electro-reduction in NO3−, with a sensitivity of 44.71 μA/mM. They had a limit of detection of 0.87 µM and a good dynamic linear concentration range from 0.05 to 3 mM. The results were compared to spectrophotometric analysis. In addition, the devices exhibited good stability and a maximum standard deviation (RSD) of 5% after ten measurements; reproducibility, with a maximum RSD of 4%; and repeatability after 10 measurements with the RSD at only 5.63%. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Low latency optical-based mode tracking with machine learning deployed on FPGAs on a tokamak.

Author

Wei, Y., Forelli, R. F., Hansen, C., Levesque, J. P., Tran, N., Agar, J. C., Di Guglielmo, G., Mauel, M. E., and Navratil, G. A.

Abstract

Active feedback control in magnetic confinement fusion devices is desirable to mitigate plasma instabilities and enable robust operation. Optical high-speed cameras provide a powerful, non-invasive diagnostic and can be suitable for these applications. In this study, we process high-speed camera data, at rates exceeding 100 kfps, on in situ field-programmable gate array (FPGA) hardware to track magnetohydrodynamic (MHD) mode evolution and generate control signals in real time. Our system utilizes a convolutional neural network (CNN) model, which predicts the n = 1 MHD mode amplitude and phase using camera images with better accuracy than other tested non-deep-learning-based methods. By implementing this model directly within the standard FPGA readout hardware of the high-speed camera diagnostic, our mode tracking system achieves a total trigger-to-output latency of 17.6 μs and a throughput of up to 120 kfps. This study at the High Beta Tokamak-Extended Pulse (HBT-EP) experiment demonstrates an FPGA-based high-speed camera data acquisition and processing system, enabling application in real-time machine-learning-based tokamak diagnostic and control as well as potential applications in other scientific domains. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Smart Pixels: towards on-sensor inference of charged particle track parameters and uncertainties

Author

Dickinson, J., primary, Kovach-Fuentes, R., additional, Gray, L., additional, Swartz, M., additional, Di Guglielmo, G., additional, Bean, A., additional, Berry, D., additional, Valentin, M., additional, DiPetrillo, K., additional, Fahim, F., additional, Hirschauer, J., additional, Kulkarni, S., additional, Lipton, R., additional, Maksimovic, P., additional, Mills, C., additional, Neubauer, M., additional, Parpillon, B., additional, Pradhan, G., additional, Syal, C., additional, Tran, N., additional, Yoo, J., additional, and Young, A., additional

Published
2023
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13. Measurement of Decay Parameters for $\Xi^{-} \to \Lambda \pi^{-}$ Decay

Author

FNAL E756 Collaboration, Chakravorty, A., Diehl, H. T., Duryea, J., Guglielmo, G., Heller, K., Ho, P. M., James, C., Johns, K., Kaplan, D. M, Longo, M. J., Luk, K. B., Rameika, R., Rubin, H. A., Teige, S., Thomson, G. B., and Zou, Y.

Subjects
High Energy Physics - Experiment
Abstract

Based on 1.35 million polarized $\Xi^{-}$ events, we measure the parameter $\phi_{\Xi}$ to be $-1.61^{\circ} \pm 2.66^{\circ} \pm 0.37^{\circ}$ for $\Xi^{-} \to \Lambda \pi^{-}$ decay. New results for the parameters $\beta_{\Xi}$ and $\gamma_{\Xi}$ are also presented. Assuming that the CP-violating phase-shift difference is negligible, we deduce the strong phase-shift difference between the P-wave and S-wave amplitudes of the $\Lambda\pi$ final state to be $3.17^{\circ} \pm 5.28^{\circ} \pm 0.73^{\circ}$. This strong phase-shift difference reduces the theoretical uncertainty in estimating the level of CP violation in $\Xi$-hyperon decay., Comment: To be published in Physical Review Letters

Published
2003
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14. Search for Direct CP Violation in Non-Leptonic Decays of Charged $\Xi$ and $\Lambda$ Hyperons

Author

Luk, K. B., Diehl, H. T., Duryea, J., Guglielmo, G., Heller, K., Ho, P. M., James, C., Johns, K., Longo, M. J., Rameika, R., Teige, S., and Thomson, G. B.

Subjects
High Energy Physics - Experiment
Abstract

A search for direct CP violation in the non-leptonic decays of hyperons has been performed. In comparing the product of the decay parameters, $\alpha_{\Xi}\alpha_{\Lambda}$, in terms of an asymmetry parameter, $A_{\Xi\Lambda}$, between hyperons and anti-hyperons in the charged $\Xi \to \Lambda \pi$ and $\Lambda \to p \pi$ decay sequence, we found no evidence of direct CP violations. The parameter $A_{\Xi\Lambda}$ was measured to be $0.012 \pm 0.014$., Comment: Submitted for publication; RevTex, 13 pages, 4 figures

Published
2000
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15. Silicon‐Based Sensing Surface for Alzheimer's Disease Diagnosis by Phages Probes.

Author

Calorenni, Paolo, Paratore, Vincenzo, Rizzo, Maria G., De Plano, Laura M., Condorelli, Guglielmo G., De Luca, Giovanna, Oddo, Salvatore, Guglielmino, Salvatore, Sciuto, Emanuele L., and Conoci, Sabrina

Subjects
ALZHEIMER'S disease, DIAGNOSIS, MEDICAL screening, NEURODEGENERATION, IMMUNOGLOBULIN G, SURFACE chemistry, BACTERIOPHAGES
Abstract

Alzheimer's disease (AD) is a diffused neurodegenerative disorder affecting people in advanced age causing loss of memory and dementia. Nowadays, diagnosis and treatment of AD are still challenging due to the lack of diagnostic systems that allow for an early and reliable diagnosis and therapy monitoring. Moreover, conventional strategies for AD diagnosis are based on brain imaging techniques that are invasive and expensive for early and massive screening. Phage display approach, using engineered phage probe for direct amyloid‐β (Aβ)‐autoantibodies detection, overcome these limitations leading to the possibility of safe and low‐cost screening. Moreover, the combination with silicon technology further improves the easiness of diagnosis due to the portability of devices and the integration of sensitive transduction signals. In this work, an innovative silicon‐based sensing technology is reported detecting Aβ‐autoantibodies, specifically Immunoglobulin G (IgG), in human sera by engineered M13‐phage probes (ADPP). The strategy hinges on a bio‐surface that is integrated on top of a silicon biosensor. Thanks to phages probes exposing Aβ‐mimic peptides, this chip can capture and reveal Aβ‐autoantibodies, discriminating between healthy and AD conditions. The surface chemistry is morphologically and chemically characterized and the phage‐based biosensor ability to recognise Aβ‐autoantibodies is proved by transduction with enzyme‐linked anti‐M13 antibodies. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Implementing machine learning methods on QICK hardware for qubit readout & control

Author

Yesilyurt, O., primary, Campos, J., additional, Di Guglielmo, G., additional, Boltasseva, A., additional, Bowring, D., additional, Cancelo, G., additional, Du, B., additional, Fahim, F., additional, Herwig, C., additional, Ma, R., additional, Perdue, G., additional, Shalaev, V., additional, Stefanazzi, L., additional, Tran, N., additional, and Uemura, S., additional

Published
2023
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18. Development of a questionnaire on nutritional knowledge for the obese hospitalized patient: the NUTRIKOB questionnaire

Author

Mambrini, S, Soranna, D, Averna, E, Di Guglielmo, G, Lucchetti, E, Tinozzi, T, Vinci, C, Barbieri, V, Zambon, A, Bertoli, S, Scacchi, M, Mambrini, SP, Mambrini, S, Soranna, D, Averna, E, Di Guglielmo, G, Lucchetti, E, Tinozzi, T, Vinci, C, Barbieri, V, Zambon, A, Bertoli, S, Scacchi, M, and Mambrini, SP

Abstract

Introduction: Different approaches, involving different areas and figures, are useful for the rehabilitation of obese subjects through a multidisciplinary hospital path. A focal point of rehabilitation is represented by education on healthy eating by increasing the dietary knowledge patients. Few tools investigating food knowledge are available in Italy: therefore, the need has emerged to develop easy-to-use tools for clinical practice that allow to detect food knowledge to set up a more targeted food re-education. The following work aimed at building and validating a questionnaire capable of investigating the dietary knowledge of the population affected by obesity. Methods: A pool of experts carried out a review of the literature, gathering all the information necessary to select and construct the best set of questions and the format of the final project of the questionnaire. During statistical analysis the validity, reproducibility and stability of the questionnaire were investigate in a sample of 450 subjects with obesity. Results: Early analysis disclosed that 5 questions of the original questionnaire had no discriminating power. The successive validation phases were successful, confirming good content validity, stability and reproducibility over time. Discussion: The questionnaire has all the characteristics to be considered a valid tool for investigating dietary knowledge in the obese population. The psychometric tests confirmed a good internal consistency of the structure, a validity of the content, a good reproducibility and stability over time.

Published
2023

19. Examining CP symmetry in strange baryon decay

Author

Luk, Kam-Biu, Fermilab E756 Collaboration, HyperCP Collaboration, Burnstein, R.A., Chakravorty, A., Chan, A., Chen, Y.C., Choong, W.S., Clark, K., Diehl, T., Dukes, E.C., Durandet, C., Duryea, J., Felix, J., Gidal, G., Guglielmo, G., Gustafson, H.R., Heller, K., Ho, C., Ho, P.M., Holmstrom, T., Huang, M., James, C., Jenkins, M., Johns, K., Jones, T., Kaplan, D.M., Lederman, L.M., Leros, N., Lopez, F., Longo, M.J., Lu, L., Luebke, W., Nelson, K., Park, H.K., Perroud, J.P., Rajaram, D., Rameika, R., Rubin, H.A., Teige, S., Teng, P.K., Thomson, G., Volks, J., White, C.G., White, S.L., and Zyla, P.

Subjects
Physics of elementary particles and fields, CP Symmetry Strange Baryon
Abstract

Non-conservation of CP symmetry can manifest itself in non-lepton ichyperon decays as a difference in the decay parameter between the strange-baryon decay and its charge conjugate. By comparing the decay distribution in the $\Lambda$ helicity frame for the decay sequence $\Xi- \to \Lambda \pi-$,$\Lambda \to p \pi-$with that of $\overline Xi+$ decay, E756 at Fermilab did not observe any CP-odd effect at the $10-2$ level. The status of a follow-up experiment, HyperCP (FNAL E871), to search for CP violation in charged $\Xi-\Lambda$ decay with a sensitivity of $10-4$is also presented.

Published
2000

20. List of Contributors

Author

Amiji, Mansoor M., primary, Araújo, Marco, additional, Asai, Tomohiro, additional, Avvakumova, Svetlana, additional, Barrias, Cristina C., additional, Budak, Hikmet, additional, Burnett, John C., additional, Cambria, Maria T., additional, Cao, Wen, additional, Carreira, Bárbara, additional, Chithrani, Devika B., additional, Colombo, Miriam, additional, Condorelli, Guglielmo G., additional, Conniot, João, additional, Csaba, Noemi S., additional, Ehmann, Falk, additional, Feijão, Tália, additional, Fernandes, Cláudia S.M., additional, Ferreira, Fabrícia Saba, additional, Florindo, Helena F., additional, Freitas, Sidónio C., additional, Galbiati, Elisabetta, additional, Gaspar, Rogério S., additional, Gonzalez-Pajuelo, Maria, additional, Huang, Leaf, additional, Huang, Peng, additional, Huang, Yan, additional, Hudalla, Gregory A., additional, Hussain, Babar, additional, Iranzo, Olga, additional, Janas, Christine, additional, Kharlamov, Alexander N., additional, Kumar, Priyadarshi, additional, Kurt, Hasan, additional, Leiro, Victoria, additional, Lin, Jing, additional, Lin, Shichao, additional, Li, Shasha, additional, Li, Zibiao, additional, Lozano, M. Victoria, additional, Martins, Maria Cristina L., additional, Matos, Ana I., additional, Mazzucchelli, Serena, additional, Moura, Liane I.F., additional, Muhammad, Faheem, additional, Oku, Naoto, additional, Parreira, Paula, additional, Pêgo, Ana Paula, additional, Peres, Carina, additional, Pita, Ruben, additional, Prosperi, Davide, additional, Rahikkala, Antti, additional, Roque, Ana C.A., additional, Rosenholm, Jessica M., additional, Rotem, Rany, additional, Sainz, Vanessa, additional, Santander-Ortega, Manuel J., additional, Santos, Hélder A., additional, Seroski, Dillon T., additional, Silva, Ana L., additional, Silva, Liana C., additional, Singh, Amit, additional, Sosnik, Alejandro, additional, Teixeira, Daniela, additional, Teixeira, Gonçalo D.G., additional, Thürmer, René, additional, Thwala, Lungile N., additional, Torres, Ana L., additional, Tudisco, Cristina, additional, Vieira, Fernanda Pires, additional, Wacker, Matthias G., additional, Wada, Akira, additional, Wei, Hui, additional, Wong, Swee Liang, additional, Wu, Jiangjiexing, additional, Yao, Jia, additional, and Yüce, Meral, additional

Published
2018
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22. Microscopic Evidence of the Behavior of pH-sensitive Food-grade Polymeric Delivery Systems

Author

Rosario Pignatello, Claudia Carbone, Salvatore Rizzo, Giuseppe Cosentino, Elide Zingale, Angela Bonaccorso, Salvatore Petralia, Francesca Monforte, and Guglielmo G. Condorelli

Subjects
Cultural Studies, Linguistics and Language, History, Anthropology, Language and Linguistics
Abstract

Background: Colon delivery systems are designed for the oral delivery of active compounds in the large intestine. Food-grade copolymers Eudraguard® Biotic (EUGB) and control (EUGC) have been investigated to develop colloidal systems loading natural active ingredients. Methods: In this study, we evaluated the degradation process of these matrices in simulated gastric, intestinal and colonic conditions. Microparticles made of EUGB and EUGC, alone or in combination, were loaded with the model compound resveratrol (RSV). A parallel study was performed on in vitro RSV release and SEM analysis of microparticles kept at different pH values. Results: All systems ensured a limited gastric release of RSV (below 20%), presenting only small pores on the surface of microparticles treated with simulated gastric fluid. EUGB microparticles showed the maximum release in simulated colon fluid (SCF), showing a complete dissolution of the microparticle matrix. The EUGC-based system allowed a prolonged release of RSV over time, and in SCF, it showed only partial degradation. Using mixed EUGB/EUGC matrices, a prolonged RSV release was observed along the intestinal tract. Conclusion: Overall, EUGB and EUGC copolymers were able to modulate and localize the release of entrapped cargo in the small intestine and colon. They could have interesting applications in treating bowel diseases synergistically with other therapeutic strategies.

Published
2023

25. In situ metalation of free base phthalocyanine covalently bonded to silicon surfaces

Author

Fabio Lupo, Cristina Tudisco, Federico Bertani, Enrico Dalcanale, and Guglielmo G. Condorelli

Subjects
metalation, phthalocyanine, silicon surface, surface functionalization, X-ray photoelectron spectroscopy (XPS), Technology, Chemical technology, TP1-1185, Science, Physics, QC1-999
Abstract

Free 4-undecenoxyphthalocyanine molecules were covalently bonded to Si(100) and porous silicon through thermic hydrosilylation of the terminal double bonds of the undecenyl chains. The success of the anchoring strategy on both surfaces was demonstrated by the combination of X-ray photoelectron spectroscopy with control experiments performed adopting the commercially available 2,3,9,10,16,17,23,24-octakis(octyloxy)-29H,31H-phthalocyanine, which is not suited for silicon anchoring. Moreover, the study of the shape of the XPS N 1s band gave relevant information on the interactions occurring between the anchored molecules and the substrates. The spectra suggest that the phthalocyanine ring interacts significantly with the flat Si surface, whilst ring–surface interactions are less relevant on porous Si. The surface-bonded molecules were then metalated in situ with Co by using wet chemistry. The efficiency of the metalation process was evaluated by XPS measurements and, in particular, on porous silicon, the complexation of cobalt was confirmed by the disappearance in the FTIR spectra of the band at 3290 cm−1 due to –NH stretches. Finally, XPS results revealed that the different surface–phthalocyanine interactions observed for flat and porous substrates affect the efficiency of the in situ metalation process.

Published
2014
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29. Porphyrin functionalized bismuth ferrite for enhanced solar light photocatalysis

Author

Guglielmo G. Condorelli, L. Pulvirenti, C. Tudisco, and Pegie Cool

Subjects
Materials science, Photochemistry, Porphyrin, Inorganic Chemistry, Chemistry, chemistry.chemical_compound, X-ray photoelectron spectroscopy, chemistry, Photocatalysis, Photodegradation, Spectroscopy, Absorption (electromagnetic radiation), Visible spectrum, Bismuth ferrite
Abstract

In this work, multiferroic bismuth ferrite (BFO) was functionalized withmeso-tetraphenylporphine-4,4 ',4 '',4 '''-tetracarboxylic acid (TCPP). This new hybrid organic-inorganic material shows an enhanced photocatalytic activity for the degradation of organic dyes as it combines the properties of BFO which is an efficient visible light photocatalyst with peculiar porphyrin absorption in visible light. The anchoring of TCPP to the OH-terminations of the BFO surface through its carboxylic tethering groups was demonstrated using X-ray photoelectron spectroscopy and FT-IR spectroscopy. The photocatalytic activity of the material was also demonstrated through the photocatalytic degradation of Methylene Blue (MB) and Rhodamine-B (Rhd-B) in water under simulated solar light illumination. The TCPP molecules anchored to BFO slightly decrease (similar to 0.06 eV) the bandgap energy of the system and act as new catalytic centres, thus improving its photocatalytic activity. A photodegradation mechanism was also proposed. This new material is reusable and stable, as it maintains an unmodified photo-activity after several MB discoloration runs.

Published
2020
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31. Fabrication of Cu(II) oxide-hydroxide nanostructures onto graphene paper by laser and thermal processes for sensitive nano-electrochemical sensing of glucose

Author

Maria Grazia Grimaldi, Stefano Boscarino, Antonino Scandurra, M. Censabella, Francesco Ruffino, and Guglielmo G. Condorelli

Subjects
Copper oxide, Materials science, Mechanical Engineering, Oxide, chemistry.chemical_element, Graphene paper, Bioengineering, General Chemistry, Copper, Laser and thermal dewetting, chemistry.chemical_compound, Blood serum, chemistry, Chemical engineering, X-ray photoelectron spectroscopy, Glucose electrochemical sensing, Mechanics of Materials, Saturated calomel electrode, General Materials Science, Dewetting, Electrical and Electronic Engineering, Graphene oxide paper
Abstract

Glucose electrochemical sensors based on nanostructures of CuO/Cu(OH)2 onto graphene paper were prepared by thermal (solid) and nanosecond pulsed laser (molten phase) dewetting of a CuO layer 6 nm thin deposited by sputtering. Dewetted systems, obtained without the use of any binder, act as array of nanoelectrodes. Solid state and molten phase dewetting produce nanostructures of copper oxide-hydroxide with different average size, shape and surface composition. Molten phase dewetting originates particles with size below 100 nm, while solid state dewetting produces particles with average size of about 200 nm. Moreover, molten phase dewetting produce drop-shaped nanostructures, conversely nanostructures derived from solid state dewetting are multifaceted. X-ray photoelectron spectroscopy (XPS) characterization revealed that the surface of nanostructures is formed by a copper(II) species CuO and Cu(OH)2. Shape of anodic branch of the cyclic voltammograms of glucose in alkali solution evidenced a convergent diffusion mechanism. Analytical performances in amperometric mode are as good as or better than other sensors based on copper oxide. Amperometric detection of glucose was done at potential as low as 0.4 V versus saturated calomel electrode by both types of electrodes. Linear range from 50 μM to 10 mM, sensitivity ranging from 7 to 43 μA cm−2 mM−1 and detection limit of 7 μM was obtained. Good analytical performances were obtained by laser dewetted electrodes with a low copper content up to 1.2 by atoms percentage of the surface. Analytical performance of the proposed electrodes is compliant for the determination of glucose both in blood serum, saliva or tear.

Published
2022

32. Dy-Doped BiFeO3 thin films: Piezoelectric and bandgap tuning

Author

Quentin Micard, Samuel Margueron, Ausrine Bartasyte, Guglielmo G. Condorelli, and Graziella Malandrino

Subjects
Chemistry (miscellaneous), General Materials Science
Abstract

A tri-metallic precursor mixture has been used to deposit Bi(1−x)DyxFeO3 film on conductive SrTiO3:Nb(100) substrates. A fine adjustment of the film composition is the key enabling an accurate tuning of the film piezoelectric and optical properties.

Published
2022

34. Dual-Functional Nano-Functionalized Titanium Scaffolds to Inhibit Bacterial Growth and Enhance Osteointegration

Author

Salvatore Petralia, Francesca Monforte, Guglielmo G. Condorelli, Giovanna Calabrese, Domenico Franco, Sabrina Conoci, Francesco Traina, Stefano Squarzoni, Calabrese G., Franco D., Petralia S., Monforte F., Condorelli G.G., Squarzoni S., Traina F., and Conoci S.

Subjects
Materials science, titanium alloy, Biocompatibility, General Chemical Engineering, chemistry.chemical_element, γFe2O3, Article, Osseointegration, biocompatibility, TiO2, General Materials Science, antibacterial properties, QD1-999, Titanium alloy, Implant failure, osteointegration, Antibacterial propertie, osteoinduction, nano-functionalization, Chemistry, chemistry, Surface modification, Implant, Antibacterial activity, Titanium, Biomedical engineering
Abstract

Implantable biomaterials play a key role for the success of orthopedic surgery procedures. However, infections remain one of the most damaging post-operative complications that lead to the implant failure. Recently, several approaches have been proposed to avoid or manage implant-associated infections. Among these, an appropriate surface functionalization to confer intrinsic antibacterial properties preserving the osteo-integration ability represents an appealing strategy for the development of innovative implant materials. Titanium and its alloys are the most used materials for manufacturing of both articular and bone skull prostheses as well as dental implants. However, to date there is still a significant clinical need to improve their bioactivity, osseointegration and antibacterial activity. In this study, titanium biomimetic scaffolds are prepared by nano-functionalization with TiO2 (Ti_TiO2) and γFe2O3 (Ti_γFe2O3). Both cytocompatibility and antibacterial activity have been evaluated. Data show that both nano-functionalized scaffolds exhibit a good antibacterial activity towards Staphylococcus aureus, reducing colony number to 99.4% (Ti_TiO2) and 99.9% (Ti_γFe2O3), respectively. In addition, an increase of both human adipose-derived mesenchymal stem cells (hADSCs) cell proliferation (up to 4.3-fold for Ti_TiO2 and 3.7-fold for Ti_γFe2O3) and differentiation has been observed. These data suggest that these nano-functionalized titanium substrates represent promising prototypes for new antimicrobial and osteoconductive biomaterials to be used in the orthopedic field to reconstruct significant bone defect.

Published
2021

36. New Synthetic Route for the Growth of α‑FeOOH/NH2‑Mil-101 Films on Copper Foil for High Surface Area Electrodes

Author

Corrado Bongiorno, Alessandra Alberti, Giovanni Mannino, Guglielmo G. Condorelli, Mario Urso, Salvo Mirabella, Francesca Monforte, and Emanuele Smecca

Subjects
Materials science, General Chemical Engineering, Composite number, Iron oxide, General Chemistry, Substrate (electronics), Amorphous solid, chemistry.chemical_compound, Chemistry, chemistry, Chemical engineering, Electrode, Hydroxide, Layer (electronics), QD1-999, FOIL method
Abstract

A novel metal organic framework (MOF)-based composite was synthesized on a Cu substrate via a two-step route. An amorphous iron oxide/hydroxide layer was first deposited on a Cu foil through a sol–...

Published
2019

37. Nitrogen doped spongy TiO2 layers for sensors application

Author

Guglielmo G. Condorelli, Emanuele Smecca, Alessandra Alberti, Thomas Bottein, Salvatore Sanzaro, David Grosso, Giovanni Mannino, and Antonino La Magna

Subjects
010302 applied physics, Materials science, Mechanical Engineering, Doping, chemistry.chemical_element, Materials Science (all), Condensed Matter Physics, Mechanics of Materials, 02 engineering and technology, Surface finish, 021001 nanoscience & nanotechnology, 01 natural sciences, Nitrogen, Volumetric flow rate, Surface-area-to-volume ratio, chemistry, Sputtering, 0103 physical sciences, Cubic centimetre, General Materials Science, Composite material, 0210 nano-technology, Porosity
Abstract

Nanostructured materials represent a breakthrough in many fields of application. For sensing, the use of structures with high surface/volume ratio is crucial to obtain a good sensitivity. Chemical growth approaches are preferred to reach this goal but thick micrometers layers are usually needed. To reduce the layer thickness, methods are required to guaranteelarge exposed surfaces in a small volume. We have indeed designed new spongy TiO2 layers by a reactive sputtering method based on a grazing-incidence source combined with the local oxidation of the sputtered species (gig-lox). The process has been implemented with a doping procedure during growth consisting of adding in the reactive environment nitrogen at low flow rate in close proximity of the growing front. We have characterized the layers in terms of porosity, roughness and electrical behaviour with respect an undoped films. The doping procedure allowed improving the electrical response of the material with the best electrical performances achieved using 2 standard cubic centimeter per minute (sccm) of nitrogen. Unexpectedly, using a higher content of nitrogen is detrimental for the electrical response of the deposited layers.

Published
2019
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38. Individual differences in members of Actinobacteria, Bacteroidetes, and Firmicutes is associated with resistance or vulnerability to addiction-like behaviors in heterogeneous stock rats

Author

Simpson, S., primary, de Guglielmo, G., additional, Brennan, M., additional, Maturin, L., additional, Peters, G., additional, Jia, H., additional, Wellmeyer, E., additional, Andrews, S., additional, Woods, L. Solberg, additional, Palmer, A. A., additional, and George, O., additional

Published
2021
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39. Leptin protects against the development and expression of cocaine addiction-like behavior in heterogenous stock rats

Author

Carrette, L.L.G., primary, Coral, C., additional, Crook, C., additional, Boomhower, B., additional, Brennan, M., additional, Ortez, C., additional, Shankar, K., additional, Simpson, S., additional, Maturin, L., additional, de Guglielmo, G., additional, Woods, L. Solberg, additional, Palmer, A. A., additional, and George, O., additional

Published
2021
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40. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Author

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Subjects
flux, macroautophagy, phagophore, stress, vacuole, Autophagosome, LC3, lysosome, neurodegeneration, cancer
Abstract

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

Published
2021

41. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Author

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Werner, Brand-Saberi, Beate, Dong, X Charlie, Kenchappa, Chandra Shekar, Li, Zuguo, Lin, Yong, Oshima, Shigeru, Rong, Yueguang, Sluimer, Judith C, Stallings, Christina L, and Tong, Chun-Kit

Abstract

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

Published
2021

42. Fast inference of Boosted Decision Trees in FPGAs for particle physics

Author

Massachusetts Institute of Technology. Department of Physics, Summers, S, Guglielmo, G Di, Duarte, J, Harris, P, Hoang, D, Jindariani, S, Kreinar, E, Loncar, V, Ngadiuba, J, Pierini, M, Rankin, D, Tran, N, Wu, Z, Massachusetts Institute of Technology. Department of Physics, Summers, S, Guglielmo, G Di, Duarte, J, Harris, P, Hoang, D, Jindariani, S, Kreinar, E, Loncar, V, Ngadiuba, J, Pierini, M, Rankin, D, Tran, N, and Wu, Z

Abstract

We describe the implementation of Boosted Decision Trees in the hls4ml library, which allows the translation of a trained model into FPGA firmware through an automated conversion process. Thanks to its fully on-chip implementation, hls4ml performs inference of Boosted Decision Tree models with extremely low latency. With a typical latency less than 100 ns, this solution is suitable for FPGA-based real-time processing, such as in the Level-1 Trigger system of a collider experiment. These developments open up prospects for physicists to deploy BDTs in FPGAs for identifying the origin of jets, better reconstructing the energies of muons, and enabling better selection of rare signal processes.

Published
2021

46. The Interplay between Fe3O4 Superparamagnetic Nanoparticles, Sodium Butyrate, and Folic Acid for Intracellular Transport

Author

Guglielmo G. Condorelli, Salvatore Saccone, Giusy Villaggio, Fulvia Sinatra, Maria Teresa Cambria, Luca Pulvirenti, Samuele Laudani, and Concetta Federico

Subjects
0301 basic medicine, folate receptors, Nanoparticle, 02 engineering and technology, Ferric Compounds, Cellular uptake, Folate receptors, Magnetic nanoparticles, Sodium butyrate, Surface functionalization, Polyethylene Glycols, lcsh:Chemistry, chemistry.chemical_compound, Drug Delivery Systems, Magnetite Nanoparticles, Receptor, lcsh:QH301-705.5, Spectroscopy, surface functionalization, Folate Receptors, GPI-Anchored, General Medicine, 021001 nanoscience & nanotechnology, Computer Science Applications, Colonic Neoplasms, 0210 nano-technology, magnetic nanoparticles, Cell Survival, Antineoplastic Agents, Butyrate, Polyethylene glycol, Adenocarcinoma, Article, Catalysis, Cell Line, Inorganic Chemistry, Magnetics, 03 medical and health sciences, Folic Acid, Cell Line, Tumor, PEG ratio, Humans, Viability assay, sodium butyrate, Physical and Theoretical Chemistry, Molecular Biology, Organic Chemistry, cellular uptake, 030104 developmental biology, chemistry, lcsh:Biology (General), lcsh:QD1-999, Biophysics, Butyric Acid
Abstract

Combined treatments which use nanoparticles and drugs could be a synergistic strategy for the treatment of a variety of cancers to overcome drug resistance, low efficacy, and high-dose-induced systemic toxicity. In this study, the effects on human colon adenocarcinoma cells of surface modified Fe3O4 magnetic nanoparticles (MNPs) in combination with sodium butyrate (NaBu), added as a free formulation, were examined demonstrating that the co-delivery produced a cytotoxic effect on malignant cells. Two different MNP coatings were investigated: a simple polyethylene glycol (PEG) layer and a mixed folic acid (FA) and PEG layer. Our results demonstrated that MNPs with FA (FA-PEG@MNPs) have a better cellular uptake than the ones without FA (PEG@MNPs), probably due to the presence of folate that acts as an activator of folate receptors (FRs) expression. However, in the presence of NaBu, the difference between the two types of MNPs was reduced. These similar behaviors for both MNPs likely occurred because of the differentiation induced by butyrate that increases the uptake of ferromagnetic nanoparticles. Moreover, we observed a strong decrease of cell viability in a NaBu dose-dependent manner. Taking into account these results, the cooperation of multifunctional MNPs with NaBu, taking into consideration the particular cancer-cell properties, can be a valuable tool for future cancer treatment.

Published
2020

47. Multifunctional magnetic nanoparticles for enhanced intracellular drug transport

Author

Elvira Fantechi, Cristina Tudisco, Guglielmo G. Condorelli, Enrico Dalcanale, Anna Alba, Antonino E. Giuffrida, Federico Bertani, Fulvia Sinatra, Claudia Innocenti, Salvatore Saccone, Maria Teresa Cambria, and Claudio Sangregorio

Subjects
chemistry.chemical_classification, Materials science, Biomedical Engineering, Cavitand, Alkyne, General Chemistry, General Medicine, confocal microscopy, Combinatorial chemistry, folic acid, chemistry.chemical_compound, chemistry, Covalent bond, poly(ethylene glycol, IRON-OXIDE NANOPARTICLES, FUNCTIONALIZED SILICON SURFACE, FOLATE RECEPTOR, HYPERTHERMIA, CAVITAND, CANCER, CELLS, DELIVERY, WATER, ACID, Organic chemistry, Surface modification, Moiety, Magnetic nanoparticles, General Materials Science, Azide, Ethylene glycol
Abstract

In this paper we report the synthesis and characterization of biocompatible multi-functional magnetic nanoparticles (MNPs) able to enhance the intracellular transport of N-methylated drugs. The Fe3O4 magnetic core was first functionalized with a mixed monolayer consisting of two different phosphonic acids having terminal acetylenic and amino groups, which provide an active platform for further functionalization with organic molecules. Then, a tetraphosphonate cavitand receptor (Tiiii) bearing an azide moiety and the N-hydroxysuccinimide (NHS) activated forms of poly(ethylene glycol) (PEG), folic acid (FA) and carboxy-X-rhodamine (Rhod) were covalently anchored on alkyne and amine moieties respectively, through 1,3-dipolar cycloaddition and EDC/NHS coupling reactions. The obtained MNPs are biocompatible and possess magnetic, luminescence and recognition properties which make them suitable for multimodal theranostic applications. In particular, combined confocal microscopy and cytotoxicity experiments showed that these multi-functional MNPs are able to recognize a specific drug "in situ'' and promote its cellular internalization, thus enhancing its efficiency.

Published
2020
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48. Silver nanoparticles on hydrolyzed spent coffee grounds (HSCG) for green antibacterial devices

Author

Guglielmo G. Condorelli, Adriana Vollaro, Federica Moccia, Giovanna Pellegrino, Pierfrancesco Cerruti, Paolo Aprea, Veronica Ambrogi, Lucia Panzella, Rossella Paolillo, Alessandra Napolitano, Marco d'Ischia, Maria Rosaria Catania, Panzella, Lucia, Cerruti, Pierfrancesco, Aprea, Paolo, Paolillo, Rossella, Pellegrino, Giovanna, Moccia, Federica, Condorelli, Guglielmo Guido, Vollaro, Adriana, Ambrogi, Veronica, Catania, Maria Rosaria, D’Ischia, Marco, and Napolitano, Alessandra

Subjects
Reducing agent, 020209 energy, Strategy and Management, Silver nanoparticle, 02 engineering and technology, Chitosan films, Industrial and Manufacturing Engineering, Matrix (chemical analysis), Green synthesis, Coffee grounds, Hydrolysis, 0202 electrical engineering, electronic engineering, information engineering, Spent coffee ground, Chitosan film, Spent coffee grounds, 0505 law, General Environmental Science, Renewable Energy, Sustainability and the Environment, Chemistry, 05 social sciences, Antimicrobial, Yield (chemistry), 050501 criminology, Green synthesi, Antioxidant, Silver nanoparticles, Nuclear chemistry
Abstract

Green and alternative strategies for the preparation of silver nanoparticles (AgNP) are the focus of considerable attention, given their wide range of applications. A clean, eco-friendly, low-cost, scalable and straightforward protocol for the production of AgNP is reported, using hydrolyzed spent coffee grounds (HSCG) both as a reducing agent and a stabilizing matrix. The sample was easily recovered in 50–60% yield by centrifugation and lyophilization. Size and morphological analyses combined with X-ray diffraction analysis indicated ca. 6% w/w incorporation of AgNP of about 20 nm size into the HSCG matrix. AgNP-HSCG exhibited efficient antioxidant properties in several chemical assays and antimicrobial activities (MIC< 0.1 mg/mL) against Gram-negative bacteria such as E. coli and P. aeruginosa. Antibacterial devices made of chitosan films containing AgNP-HSCG were also prepared that showed significant inhibition zones against the same bacterial strains, even at the lowest loading (0.3% w/w) of AgNP, and remarkable antioxidant properties in aqueous media. An economic evaluation of the whole process was carried out, demonstrating that manufacturing of both HSCG and chitosan-based antibacterial devices is a convenient and easy-to-perform process, with the potential of full industrial scalability. Overall, the adopted circular approach, designed to reuse a cheap and abundant agri-food waste while avoiding the use of toxic or noxious chemicals, makes HSCG a practical and easily accessible multifunctional material for the clean and sustainable synthesis of AgNP with adequate size and stability for incorporation in efficient antioxidants and antimicrobial devices.

Published
2020
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49. Agile SoC Development with Open ESP : Invited Paper

Author

Mantovani, P., Giri, D., Di Guglielmo, G., Piccolboni, L., Zuckerman, J., Cota, E. G., Petracca, M., Pilato, C., and Carloni, L. P.

Subjects
accelerators, System-level design, SoC, network-on-chip
Published
2020

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