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11. Weaned piglets display low gastrointestinal digestion of pea (Pisum sativum L.) lectin and pea albumin 2

Author

Le Gall, M., Quillien, L., Seve, B., Gueguen, J., and Lalles, J.P.

Subjects
Peas -- Nutritional aspects, Swine -- Food and nutrition, Swine -- Physiological aspects, Polypeptides -- Properties, Serum albumin -- Properties, Proteins -- Properties, Zoology and wildlife conservation
Abstract

A study was conducted to investigate the biochemistry of digestion of field pea (Pisum sativum L.) albumins and globulins in the stomach and along the small intestine of weaned piglets with a particular emphasis on the respective roles of these compartments in pea protein digestion. Twenty-four piglets were weaned at 28 d of age. They were allocated to 2 diets (control and pea) and 3 slaughter times (3, 6, or 9 h after the last meal) in a 2 x 3 factorial arrangement of treatments in a randomized complete block design. Pea flour provided 30% of total dietary protein in the pea diet. The diets were fed for 2 wk after weaning. After slaughter, gastrointestinal tract (GIT) compartments were weighed, digesta were collected, and pH was measured. Digesta from the stomach and cranial, middle, and caudal small intestine (SI) were extracted for soluble proteins and analyzed for specific pea proteins using SDS-PAGE, immunoblotting, and mass spectrometry. Tissue weight of the whole GIT (P = 0.015), cecum (P < 0.001), and colon (P < 0.001) was greater in the pea diet. Digesta pH in the stomach and caudal SI was lower (P = 0.02) in the pea diet than the control diet. In the stomach, vicilin, lectin, and pea albumin 2 were not digested, whereas legumin was only partly digested. Legumin and vicilin were totally digested in the SI in less than 3 h. A resistant peptide of 15 kDa located at the N-terminus of pea albumin 2 was transiently detected at 3 h. A protein band at 20 kDa was consistently identified as lectin. It was present in high intensity in intestinal digesta of pea-fed piglets at all times after the meal compared with those fed the control diet (P < 0.001). Various proteins of, presumably, endogenous origin displayed differential digestion patterns between the control and the pea-fed piglets (P < 0.05). In conclusion, differences in digestion between specific pea proteins were observed along the GIT of piglets. They could be partly explained by differences in protein digestion in the stomach. Key words: albumin, digestion, globulin, pea protein, pig

Published
2007

12. Banff 2019 Meeting Report: Molecular diagnostics in solid organ transplantation–Consensus for the Banff Human Organ Transplant (B-HOT) gene panel and open source multicenter validation

Author

Mengel, M, Loupy, A., Haas, M. (Mark), Roufosse, C., Naesens, M, Akalin, E., Clahsen-van Groningen, M.C. (Marian), Dagobert, J., Demetris, A.J., Duong van Huyen, J.P., Gueguen, J., Issa, F., Robin, B., Rosales, I., von der Thüsen, J.H., Sanchez-Fueyo, A. (Albert), Smith, R.N., Wood, K., Adam, B., Colvin, R.B., Mengel, M, Loupy, A., Haas, M. (Mark), Roufosse, C., Naesens, M, Akalin, E., Clahsen-van Groningen, M.C. (Marian), Dagobert, J., Demetris, A.J., Duong van Huyen, J.P., Gueguen, J., Issa, F., Robin, B., Rosales, I., von der Thüsen, J.H., Sanchez-Fueyo, A. (Albert), Smith, R.N., Wood, K., Adam, B., and Colvin, R.B.

Abstract

This meeting report from the XV Banff conference describes the creation of a multiorgan transplant gene panel by the Banff Molecular Diagnostics Working Group (MDWG). This Banff Human Organ Transplant (B-HOT) panel is the culmination of previous work by the MDWG to identify a broadly useful gene panel based on whole transcriptome technology. A data-driven process distilled a gene list from peer-reviewed comprehensive microarray studies that discovered and validated their use in kidney, liver, heart, and lung transplant biopsies. These were supplemented by genes that define relevant cellular pathways and cell types plus 12 reference genes used for normalization. The 770 gene B-HOT panel includes the most pertinent genes related to rejection, tolerance, viral infections, and innate and adaptive immune responses. This commercially available panel uses the NanoString platform, which can quantitate transcripts from formalin-fixed paraffin-embedded samples. The B-HOT panel will facilitate multicenter collaborative clinical research using archival samples and permit the development of an open source large database of standardized analyses, thereby expediting clinical validation studies. The MDWG believes that a pathogenesis and pathway based molecular approach will be valuable for investigators and promote therapeutic decision-making and clinical trials.

Published
2020
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13. The Banff 2019 Kidney Meeting Report (I): Updates on and clarification of criteria for T cell– and antibody-mediated rejection

Author

Loupy, A. (Alexandre), Haas, M. (Mark), Roufosse, C. (Candice), Naesens, L., Adam, B. (Benjamin), Afrouzian, M. (Marjan), Akalin, E. (Enver), Alachkar, N. (Nada), Bagnasco, S. (Serena), Becker, J.U. (Jan U.), Cornell, L.D. (Lynn D.), Clahsen-van Groningen, M.C. (Marian), Demetris, A.J. (Anthony J.), Dragun, D. (Duska), Duong van Huyen, J.-P. (Jean-Paul), Farris, A.B. (Alton), Fogo, A.B. (Agnes), Gibson, I.W. (Ian), Glotz, D. (Denis), Gueguen, J. (Juliette), Kikic, Z. (Zeljko), Kozakowski, N. (Nicolas), Kraus, E. (Edward), Lefaucheur, C. (Carmen), Liapis, H. (Helen), Mannon, R.B. (Roslyn), Montgomery, R.A. (Robert A.), Nankivell, B.J. (Brian J.), Nickeleit, V. (Volker), Nickerson, P. (Peter), Rabant, M. (Marion), Racusen, L. (Lorraine), Randhawa, P. (Parmjeet), Robin, B. (Blaise), Rosales, I.A. (Ivy A.), Sapir-Pichhadze, R. (Ruth), Schinstock, C.A. (Carrie A.), Seron, D. (Daniel), Singh, H.K. (Harsharan K.), Smith, R.N. (Rex N.), Stegall, M.D. (Mark D.), Zeevi, A. (Adriana), Solez, K. (Kim), Colvin, R.B. (Robert B.), Mengel, M. (Michael), Loupy, A. (Alexandre), Haas, M. (Mark), Roufosse, C. (Candice), Naesens, L., Adam, B. (Benjamin), Afrouzian, M. (Marjan), Akalin, E. (Enver), Alachkar, N. (Nada), Bagnasco, S. (Serena), Becker, J.U. (Jan U.), Cornell, L.D. (Lynn D.), Clahsen-van Groningen, M.C. (Marian), Demetris, A.J. (Anthony J.), Dragun, D. (Duska), Duong van Huyen, J.-P. (Jean-Paul), Farris, A.B. (Alton), Fogo, A.B. (Agnes), Gibson, I.W. (Ian), Glotz, D. (Denis), Gueguen, J. (Juliette), Kikic, Z. (Zeljko), Kozakowski, N. (Nicolas), Kraus, E. (Edward), Lefaucheur, C. (Carmen), Liapis, H. (Helen), Mannon, R.B. (Roslyn), Montgomery, R.A. (Robert A.), Nankivell, B.J. (Brian J.), Nickeleit, V. (Volker), Nickerson, P. (Peter), Rabant, M. (Marion), Racusen, L. (Lorraine), Randhawa, P. (Parmjeet), Robin, B. (Blaise), Rosales, I.A. (Ivy A.), Sapir-Pichhadze, R. (Ruth), Schinstock, C.A. (Carrie A.), Seron, D. (Daniel), Singh, H.K. (Harsharan K.), Smith, R.N. (Rex N.), Stegall, M.D. (Mark D.), Zeevi, A. (Adriana), Solez, K. (Kim), Colvin, R.B. (Robert B.), and Mengel, M. (Michael)

Abstract

The XV. Banff conference for allograft pathology was held in conjunction with the annual meeting of the American Society for Histocompatibility and Immunogenetics in Pittsburgh, PA (USA) and focused on refining recent updates to the classification, advances from the Banff working groups, and standardization of molecular diagnostics. This report on kidney transplant pathology details clarifications and refinements to the criteria for chronic active (CA) T cell–mediated rejection (TCMR), borderline, and antibody-mediated rejection (ABMR). The main focus of kidney sessions was on how to address biopsies meeting criteria for CA TCMR plus borderline or acute TCMR. Recent studies on the clinical impact of borderline infiltrates were also presented to clarify whether the threshold for interstitial inflammation in diagnosis of borderline should be i0 or i1. Sessions on ABMR focused on biopsies showing microvascular inflammation in the absence of C4d staining or detectable donor-specific antibodies; the potential value of molecular diagnostics in such cases and recommendations for use of the latter in the setting of solid organ transplantation are presented in the accompanying meeting report. Finally, several speakers discussed the capabilities of artificial intelligence and the potential for use of machine learning algorithms in diagnosis and personalized therapeutics in solid organ transplantation.

Published
2020
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14. The Banff 2019 Kidney Meeting Report (I): Updates on and clarification of criteria for T cell– and antibody-mediated rejection

Author

Loupy, A, de Haas, M, Roufosse, C, Naesens, M, Adam, B, Afrouzian, M, Akalin, E, Alachkar, N, Bagnasco, S, Becker, JU, Cornell, LD, Groningen, Marian, Demetris, AJ, Dragun, D, Duong van Huyen, JP, Farris, AB, Fogo, AB, Gibson, IW, Glotz, D, Gueguen, J, Kikic, Z, Kozakowski, N, Kraus, E, Lefaucheur, C, Liapis, H, Mannon, RB, Montgomery, RA, Nankivell, BJ, Nickeleit, V, Nickerson, P, Rabant, M, Racusen, L, Randhawa, P, Robin, B, Rosales, IA, Sapir-Pichhadze, R, Schinstock, CA, Seron, D, Singh, HK, Smith, RN, Stegall, MD, Zeevi, A, Solez, K, Colvin, RB, Mengel, M, Loupy, A, de Haas, M, Roufosse, C, Naesens, M, Adam, B, Afrouzian, M, Akalin, E, Alachkar, N, Bagnasco, S, Becker, JU, Cornell, LD, Groningen, Marian, Demetris, AJ, Dragun, D, Duong van Huyen, JP, Farris, AB, Fogo, AB, Gibson, IW, Glotz, D, Gueguen, J, Kikic, Z, Kozakowski, N, Kraus, E, Lefaucheur, C, Liapis, H, Mannon, RB, Montgomery, RA, Nankivell, BJ, Nickeleit, V, Nickerson, P, Rabant, M, Racusen, L, Randhawa, P, Robin, B, Rosales, IA, Sapir-Pichhadze, R, Schinstock, CA, Seron, D, Singh, HK, Smith, RN, Stegall, MD, Zeevi, A, Solez, K, Colvin, RB, and Mengel, M

Published
2020

22. Process Assessment Issues in a Bachelor Capstone Project

Author

Ribaud, V., Bescond, A., Gourvenec, M., Gueguen, J., Lamour, V., Levieux, A., Parvillers, T., Rory O'Connor, Ribaud, Vincent, Laboratoire des sciences et techniques de l'information, de la communication et de la connaissance (Lab-STICC), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Télécom Bretagne-Institut Brestois du Numérique et des Mathématiques (IBNM), Université de Brest (UBO)-Université européenne de Bretagne - European University of Brittany (UEB)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), School of Computing [Dublin], and Dublin City University [Dublin] (DCU)

Subjects
process assessment, Self-assessment, [INFO.INFO-SE] Computer Science [cs]/Software Engineering [cs.SE], ComputingMilieux_COMPUTERSANDEDUCATION, [INFO.INFO-SE]Computer Science [cs]/Software Engineering [cs.SE]
Abstract

International audience; Based on a small subset of ISO/IEC 15504:2006, a Process Assessment was performed in the capstone project of a Bachelor in Computer Science. Parallel to this assessment, students performed a continuous self-assessment using an ability model based on 15504 Base Practices and Work Products. This paper highlights how students' self-assessment and teacher's assessment are correlated. The capstone project itself implements major constructivism principles. This paper presents also the students' point of view through different questionnaires and students' participation to the paper.

Published
2015

23. The 11q;22q translocation: A European collaborative analysis of 43 cases

Author

Fraccaro, M., Lindsten, J., Ford, C. E., Iselius, L., Antonelli, A., Aula, P., Aurias, A., Bain, A. D., Bartsch-Sandhoff, M., Bernardi, F., Boyd, E., Buchanan, L. F., Cameron, A. H., de la Chapelle, A., Ciuffa, G., Cuoco, C., Dutrillaux, B., Dutton, G., Ferguson-Smith, M. A., Francesconi, D., Geraedts, J. P. M., Gimelli, G., Gueguen, J., Gärsner, E., Hagemeijer, A., Hansen, F. J., Hollings, P. E., Hustinx, T. W. J., Kaakinen, A., van de Kamp, J. J. P., von Koskull, H., Lejeune, J., Lindenbaum, R. H., McCreanor, H. H., Mikkelsen, M., Mitelman, F., Nicoletti, B., Nilsby, J., Nilsson, B., Noel, B., Padovani, E., Pasquali, F., de Pater, J., Pedersen, C., Petersen, F., Robson, E. B., Rotman, J., Ryynänen, M., Sachs, E., Salat, J., Smythe, R. H., Stabell, I., Šubrt, I., Vampirelli, P., Wessner, G., Zergollern, L., and Zuffardi, O.

Published
1980
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36. Variability in Protein Composition of Pea Seed Studied by FPLC and Multidimensional Analysis

Author

Baniel, A., Bertrand, D., Lelion, A., and Gueguen, J.

Subjects
Peas -- Health aspects -- Research, Plant proteins -- Research -- Health aspects, Liquid chromatography -- Research -- Health aspects, Agricultural industry, Business, Research, Health aspects
Abstract

The nutritional and the functional quality of pea (Pisum sativum L.) seed depends primarily on their protein content and composition. For breeding as well as for industrial uses, efficient methods for analyzing protein composition are of great importance, in the present work, a procedure combining fast protein liquid chromatography and statistical analysis was developed for routine determination of protein composition in pea seed. Chromatographic conditions optimized the resolution and repeatability of peak areas and peak retention times sufficiently for statistical analysis. The analytical procedure was a practical compromise between the time of chromatographic separation and the repeatability of the fractionation. The method utilizes the variability among pea cultivars and the repeatability of their chromatographic profiles and can characterize seed protein composition with sufficient precision to distinguish cultivars., Pea is a long established and significant crop in Europe, but its production has been rather low for a long time. The increasing need of protein-rich raw materials for animal [...]

Published
1998

38. ChemInform Abstract: Betulinic Acid Derivatives: A New Class of Human Immunodeficiency Virus Type 1 Specific Inhibitors with a New Mode of Action.

Author

EVERS, M., primary, POUJADE, C., additional, SOLER, F., additional, RIBEILL, Y., additional, JAMES, C., additional, LELIEVRE, Y., additional, GUEGUEN, J.‐C., additional, REISDORF, D., additional, MORIZE, I., additional, PAUWELS, R., additional, DE CLERCQ, E., additional, HENIN, Y., additional, BOUSSEAU, A., additional, MAYAUX, J.‐F., additional, LE PECQ, J.‐B., additional, and DEREU, N., additional

Published
1996
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47. Properties and Microstructure of Thermo-Pressed Wheat Gluten Films:  a Comparison with Cast Films

Author

Mangavel, C., Rossignol, N., Perronnet, A., Barbot, J., Popineau, Y., and Gueguen, J.

Abstract

Wheat gluten films were prepared by thermo-pressing, and their mechanical properties were compared to those of cast films. The stress−strain relationship was established for films with various amounts of glycerol. Both relationships were quite different, revealing a different network organization. Thermo-pressed films presented higher stress values than cast films, but the effect of the glycerol amount was similar in both cases, an increase of the glycerol amount leading to a decrease of both films stress. The glycerol influence on the strain at break of thermo-pressed films was very limited, with strain values reaching a maximum around 200%. The role of disulfide bridges on themomoulded films mechanical properties was investigated, and it was shown that some rearrangements and a significative protein insolubilization occurred during the process. The effective flow porosity of the protein network for thermo-pressed films was estimated by water capillary rise measurements to about 7%. Scanning electron microscopy was used to obtain some information about the microstructure of both cast and thermo-pressed films.

Published
2004

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