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2. Predictors for Prolonged Hospital Stay Solely to Complete Intravenous Antifungal Treatment in Patients with Candidemia: Results from the ECMM Candida III Multinational European Observational Cohort Study

Author

Egger, Matthias, Salmanton-García, Jon, Barac, Aleksandra, Gangneux, Jean-Pierre, Guegan, Hélène, Arsic-Arsenijevic, Valentina, Matos, Tadeja, Tomazin, Rok, Klimko, Nikolai, Bassetti, Matteo, Hammarström, Helena, Meijer, Eelco F. J., Meis, Jacques F., Prattes, Juergen, Krause, Robert, Resat Sipahi, Oguz, Scharmann, Ulrike, White, P. Lewis, Desoubeaux, Guillaume, García-Rodríguez, Julio, Garcia-Vidal, Carolina, Martín-Pérez, Sonia, Ruiz, Maite, Tumbarello, Mario, Talento, Alida Fe, Rogers, Benedict, Lagrou, Katrien, van Praet, Jens, Arikan-Akdagli, Sevtap, Arendrup, Maiken C., Koehler, Philipp, Cornely, Oliver A., and Hoenigl, Martin

Published
2023
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5. Guideline adherence and survival of patients with candidaemia in Europe: results from the ECMM Candida III multinational European observational cohort study

Author

Tumbarello, Mario, Talento, Alida Fe, Ruiz, Alba C, Racil, Zdenek, Stoma, Igor, Calbacho, Maria, Van Wijngaerden, Eric, Henriques, Júlia, Jordan, Harriett, Ferroni, Valentina, Ozyurt, Ozlem Koyuncu, Milacek, Christopher, Krause, Robert, Zurl, Christoph, Backx, Matthijs, Li, Ang, Seufert, Raphael, Tomazin, Rok, Blankenheim, Yael, Dávila-Valls, Julio, García-Clemente, Paloma, Freiberger, Tomas, Buil, Jochem, Meis, Jacques F, Akyol, Deniz, Guegan, Hélène, Logan, Clare, Hoenigl, Martin, Salmanton-García, Jon, Egger, Matthias, Gangneux, Jean-Pierre, Bicanic, Tihana, Arikan-Akdagli, Sevtap, Alastruey-Izquierdo, Ana, Klimko, Nikolai, Barac, Aleksandra, Özenci, Volkan, Meijer, Eelco F J, Khanna, Nina, Bassetti, Matteo, Rautemaa-Richardson, Riina, Lagrou, Katrien, Adam, Kai-Manuel, Akalin, Emin Halis, Akova, Murat, Arsic Arsenijevic, Valentina, Aujayeb, Avinash, Blennow, Ola, Bretagne, Stéphane, Danion, François, Denis, Blandine, de Jonge, Nick Alexander, Desoubeaux, Guillaume, Drgona, Lubos, Erben, Nurettin, Gori, Andrea, García Rodríguez, Julio, Garcia-Vidal, Carolina, Giacobbe, Daniele Roberto, Goodman, Anna L, Hamal, Petr, Hammarström, Helena, Toscano, Cristina, Lanternier, Fanny, Lass-Flörl, Cornelia, Lockhart, Deborah E A, Longval, Thomas, Loughlin, Laura, Matos, Tadeja, Mikulska, Malgorzata, Narayanan, Manjusha, Martín-Pérez, Sonia, Prattes, Juergen, Rogers, Benedict, Rahimli, Laman, Ruiz, Maite, Roilides, Emmanuel, Samarkos, Michael, Scharmann, Ulrike, Sili, Uluhan, Sipahi, Oguz Resat, Sivakova, Alena, Steinmann, Joerg, Trauth, Janina, Turhan, Ozge, Van Praet, Jens, Vena, Antonio, White, P Lewis, Willinger, Birgit, Tortorano, Anna Maria, Arendrup, Maiken C, Koehler, Philipp, and Cornely, Oliver A

Published
2023
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10. Fungal infections in mechanically ventilated patients with COVID-19 during the first wave: the French multicentre MYCOVID study

Author

Gangneux, Jean-Pierre, Dannaoui, Eric, Fekkar, Arnaud, Luyt, Charles-Edouard, Botterel, Françoise, De Prost, Nicolas, Tadié, Jean-Marc, Reizine, Florian, Houzé, Sandrine, Timsit, Jean-François, Iriart, Xavier, Riu-Poulenc, Béatrice, Sendid, Boualem, Nseir, Saad, Persat, Florence, Wallet, Florent, Le Pape, Patrice, Canet, Emmanuel, Novara, Ana, Manai, Melek, Cateau, Estelle, Thille, Arnaud W, Brun, Sophie, Cohen, Yves, Alanio, Alexandre, Mégarbane, Bruno, Cornet, Muriel, Terzi, Nicolas, Lamhaut, Lionel, Sabourin, Estelle, Desoubeaux, Guillaume, Ehrmann, Stephan, Hennequin, Christophe, Voiriot, Guillaume, Nevez, Gilles, Aubron, Cécile, Letscher-Bru, Valérie, Meziani, Ferhat, Blaize, Marion, Mayaux, Julien, Monsel, Antoine, Boquel, Frédérique, Robert-Gangneux, Florence, Le Tulzo, Yves, Seguin, Philippe, Guegan, Hélène, Autier, Brice, Lesouhaitier, Matthieu, Pelletier, Romain, Belaz, Sorya, Bonnal, Christine, Berry, Antoine, Leroy, Jordan, François, Nadine, Richard, Jean-Christophe, Paulus, Sylvie, Argaud, Laurent, Dupont, Damien, Menotti, Jean, Morio, Florent, Soulié, Marie, Schwebel, Carole, Garnaud, Cécile, Guitard, Juliette, Le Gal, Solène, Quinio, Dorothée, Morcet, Jeff, Laviolle, Bruno, Zahar, Jean-Ralph, and Bougnoux, Marie-Elisabeth

Published
2022
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19. Invasive fungal diseases in patients with autoimmune diseases: a case series from the French RESSIF network

Author

Galmiche, Simon, primary, Thoreau, Benjamin, additional, Bretagne, Stéphane, additional, Alanio, Alexandre, additional, Paugam, André, additional, Letscher-Bru, Valérie, additional, Cassaing, Sophie, additional, Gangneux, Jean-Pierre, additional, Guegan, Hélène, additional, Favennec, Loïc, additional, Minoza, Alida, additional, Morio, Florent, additional, Bonhomme, Julie, additional, Desoubeaux, Guillaume, additional, Eloy, Odile, additional, Hasseine, Lilia, additional, Sasso, Milène, additional, Millon, Laurence, additional, Bellanger, Anne-Pauline, additional, Poirier, Philippe, additional, Moniot, Maxime, additional, Chouaki, Taieb, additional, Huguenin, Antoine, additional, Dalle, Frédéric, additional, Bouteille, Bernard, additional, Nicolas, Muriel, additional, Desbois-Nogard, Nicole, additional, Bougnoux, Marie-Elisabeth, additional, Danion, François, additional, Poindron, Vincent, additional, Néel, Antoine, additional, Boukris-Sitbon, Karine, additional, Lanternier, Fanny, additional, and Terrier, Benjamin, additional

Published
2023
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20. Mycobiome analyses of critically ill COVID-19 patients

Author

Weaver, Danielle, primary, Gago, Sara, additional, Bassetti, Matteo, additional, Giacobbe, Daniele Roberto, additional, Prattes, Juergen, additional, Hoenigl, Martin, additional, Reizine, Florian, additional, Guegan, Hélène, additional, Gangneux, Jean-Pierre, additional, Bromley, Michael John, additional, and Bowyer, Paul, additional

Published
2023
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21. Guideline adherence and survival of patients with candidaemia in Europe: results from the ECMM Candida III multinational European observational cohort study

Author

Hoenigl, Martin, primary, Salmanton-García, Jon, additional, Egger, Matthias, additional, Gangneux, Jean-Pierre, additional, Bicanic, Tihana, additional, Arikan-Akdagli, Sevtap, additional, Alastruey-Izquierdo, Ana, additional, Klimko, Nikolai, additional, Barac, Aleksandra, additional, Özenci, Volkan, additional, Meijer, Eelco F J, additional, Khanna, Nina, additional, Bassetti, Matteo, additional, Rautemaa-Richardson, Riina, additional, Lagrou, Katrien, additional, Adam, Kai-Manuel, additional, Akalin, Emin Halis, additional, Akova, Murat, additional, Arsic Arsenijevic, Valentina, additional, Aujayeb, Avinash, additional, Blennow, Ola, additional, Bretagne, Stéphane, additional, Danion, François, additional, Denis, Blandine, additional, de Jonge, Nick Alexander, additional, Desoubeaux, Guillaume, additional, Drgona, Lubos, additional, Erben, Nurettin, additional, Gori, Andrea, additional, García Rodríguez, Julio, additional, Garcia-Vidal, Carolina, additional, Giacobbe, Daniele Roberto, additional, Goodman, Anna L, additional, Hamal, Petr, additional, Hammarström, Helena, additional, Toscano, Cristina, additional, Lanternier, Fanny, additional, Lass-Flörl, Cornelia, additional, Lockhart, Deborah E A, additional, Longval, Thomas, additional, Loughlin, Laura, additional, Matos, Tadeja, additional, Mikulska, Malgorzata, additional, Narayanan, Manjusha, additional, Martín-Pérez, Sonia, additional, Prattes, Juergen, additional, Rogers, Benedict, additional, Rahimli, Laman, additional, Ruiz, Maite, additional, Roilides, Emmanuel, additional, Samarkos, Michael, additional, Scharmann, Ulrike, additional, Sili, Uluhan, additional, Sipahi, Oguz Resat, additional, Sivakova, Alena, additional, Steinmann, Joerg, additional, Trauth, Janina, additional, Turhan, Ozge, additional, Van Praet, Jens, additional, Vena, Antonio, additional, White, P Lewis, additional, Willinger, Birgit, additional, Tortorano, Anna Maria, additional, Arendrup, Maiken C, additional, Koehler, Philipp, additional, Cornely, Oliver A, additional, Tumbarello, Mario, additional, Talento, Alida Fe, additional, Ruiz, Alba C, additional, Racil, Zdenek, additional, Stoma, Igor, additional, Calbacho, Maria, additional, Van Wijngaerden, Eric, additional, Henriques, Júlia, additional, Jordan, Harriett, additional, Ferroni, Valentina, additional, Ozyurt, Ozlem Koyuncu, additional, Milacek, Christopher, additional, Krause, Robert, additional, Zurl, Christoph, additional, Backx, Matthijs, additional, Li, Ang, additional, Seufert, Raphael, additional, Tomazin, Rok, additional, Blankenheim, Yael, additional, Dávila-Valls, Julio, additional, García-Clemente, Paloma, additional, Freiberger, Tomas, additional, Buil, Jochem, additional, Meis, Jacques F, additional, Akyol, Deniz, additional, Guegan, Hélène, additional, and Logan, Clare, additional

Published
2023
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26. Predictors for Prolonged Hospital Stay Solely to Complete Intravenous Antifungal Treatment in Patients with Candidemia: Results from the ECMM Candida III Multinational European Observational Cohort Study

Author

Medical University of Graz, Egger, Matthias, Salmanton-García, Jon, Barac, Aleksandra, Gangneux, Jean-Pierre, Guegan, Hélène, Arsic-Arsenijevic, Valentina, Matos, Tadeja, Tomazin, Rok, Klimko, Nikolai, Bassetti, Matteo, Hammarström, Helena, Meijer, Eelco F. J., Meis, Jacques F., Prattes, Juergen, Krause, Robert, Sipahi, Oguz Resat, Scharmann, Ulrike, White, P. Lewis, Desoubeaux, Guillaume, García-Rodríguez, Julio, García-Vidal, Carolina, Martín-Pérez, Sonia, Maite Ruiz, Tumbarello, Mario, Talento, Alida Fe, Rogers, Benedict, Lagrou, Katrien, Praet, Jens van, Arikan-Akdagli, Sevtap, Arendrup, Maiken C., Koehler, Philipp, Cornely, Oliver A., Hoenigl, Martin, ECMM Candida III Study Group, Medical University of Graz, Egger, Matthias, Salmanton-García, Jon, Barac, Aleksandra, Gangneux, Jean-Pierre, Guegan, Hélène, Arsic-Arsenijevic, Valentina, Matos, Tadeja, Tomazin, Rok, Klimko, Nikolai, Bassetti, Matteo, Hammarström, Helena, Meijer, Eelco F. J., Meis, Jacques F., Prattes, Juergen, Krause, Robert, Sipahi, Oguz Resat, Scharmann, Ulrike, White, P. Lewis, Desoubeaux, Guillaume, García-Rodríguez, Julio, García-Vidal, Carolina, Martín-Pérez, Sonia, Maite Ruiz, Tumbarello, Mario, Talento, Alida Fe, Rogers, Benedict, Lagrou, Katrien, Praet, Jens van, Arikan-Akdagli, Sevtap, Arendrup, Maiken C., Koehler, Philipp, Cornely, Oliver A., Hoenigl, Martin, and ECMM Candida III Study Group

Abstract

[Background] To date, azoles represent the only viable option for oral treatment of invasive Candida infections, while rates of azole resistance among non-albicans Candida spp. continue to increase. The objective of this sub-analysis of the European multicenter observational cohort study Candida III was to describe demographical and clinical characteristics of the cohort requiring prolonged hospitalization solely to complete intravenous (iv) antifungal treatment (AF Tx)., [Methods] Each participating hospital (number of eligible hospitals per country determined by population size) included the first ~ 10 blood culture proven adult candidemia cases occurring consecutively after July 1st, 2018, and treating physicians answered the question on whether hospital stay was prolonged only for completion of intravenous antifungal therapy. Descriptive analyses as well as binary logistic regression was used to assess for predictors of prolonged hospitalization solely to complete iv AF Tx., [Findings] Hospital stay was prolonged solely for the completion of iv AF Tx in 16% (100/621) of candidemia cases by a median of 16 days (IQR 8 – 28). In the multivariable model, initial echinocandin treatment was a positive predictor for prolonged hospitalization to complete iv AF Tx (aOR 2.87, 95% CI 1.55 – 5.32, p < 0.001), while (i) neutropenia, (ii) intensive care unit admission, (iii) catheter related candidemia, (iv) total parenteral nutrition, and (v) C. parapsilosis as causative pathogen were found to be negative predictors (aOR 0.22 – 0.45; p < 0.03)., [Interpretation] Hospital stays were prolonged due to need of iv AF Tx in 16% of patients with candidemia. Those patients were more likely to receive echinocandins as initial treatment and were less severely ill and less likely infected with C. parapsilosis.

Published
2023

27. Predictors for Prolonged Hospital Stay Solely to Complete Intravenous Antifungal Treatment in Patients with Candidemia:Results from the ECMM Candida III Multinational European Observational Cohort Study

Author

Egger, Matthias, Salmanton-García, Jon, Barac, Aleksandra, Gangneux, Jean Pierre, Guegan, Hélène, Arsic-Arsenijevic, Valentina, Matos, Tadeja, Tomazin, Rok, Klimko, Nikolai, Bassetti, Matteo, Hammarström, Helena, Meijer, Eelco F.J., Meis, Jacques F., Prattes, Juergen, Krause, Robert, Resat Sipahi, Oguz, Scharmann, Ulrike, White, P. Lewis, Desoubeaux, Guillaume, García-Rodríguez, Julio, Garcia-Vidal, Carolina, Martín-Pérez, Sonia, Ruiz, Maite, Tumbarello, Mario, Talento, Alida Fe, Rogers, Benedict, Lagrou, Katrien, van Praet, Jens, Arikan-Akdagli, Sevtap, Arendrup, Maiken C., Koehler, Philipp, Cornely, Oliver A., Hoenigl, Martin, Egger, Matthias, Salmanton-García, Jon, Barac, Aleksandra, Gangneux, Jean Pierre, Guegan, Hélène, Arsic-Arsenijevic, Valentina, Matos, Tadeja, Tomazin, Rok, Klimko, Nikolai, Bassetti, Matteo, Hammarström, Helena, Meijer, Eelco F.J., Meis, Jacques F., Prattes, Juergen, Krause, Robert, Resat Sipahi, Oguz, Scharmann, Ulrike, White, P. Lewis, Desoubeaux, Guillaume, García-Rodríguez, Julio, Garcia-Vidal, Carolina, Martín-Pérez, Sonia, Ruiz, Maite, Tumbarello, Mario, Talento, Alida Fe, Rogers, Benedict, Lagrou, Katrien, van Praet, Jens, Arikan-Akdagli, Sevtap, Arendrup, Maiken C., Koehler, Philipp, Cornely, Oliver A., and Hoenigl, Martin

Abstract

Background To date, azoles represent the only viable option for oral treatment of invasive Candida infections, while rates of azole resistance among non-albicans Candida spp. continue to increase. The objective of this sub-analysis of the European multicenter observational cohort study Candida III was to describe demographical and clinical characteristics of the cohort requiring prolonged hospitalization solely to complete intravenous (iv) antifungal treatment (AF Tx). Methods Each participating hospital (number of eligible hospitals per country determined by population size) included the first ~ 10 blood culture proven adult candidemia cases occurring consecutively after July 1st, 2018, and treating physicians answered the question on whether hospital stay was prolonged only for completion of intravenous antifungal therapy. Descriptive analyses as well as binary logistic regression was used to assess for predictors of prolonged hospitalization solely to complete iv AF Tx. Findings Hospital stay was prolonged solely for the completion of iv AF Tx in 16% (100/621) of candidemia cases by a median of 16 days (IQR 8 – 28). In the multivariable model, initial echinocandin treatment was a positive predictor for prolonged hospitalization to complete iv AF Tx (aOR 2.87, 95% CI 1.55 – 5.32, p < 0.001), while (i) neutropenia, (ii) intensive care unit admission, (iii) catheter related candidemia, (iv) total parenteral nutrition, and (v) C. parapsilosis as causative pathogen were found to be negative predictors (aOR 0.22 – 0.45; p < 0.03). Interpretation Hospital stays were prolonged due to need of iv AF Tx in 16% of patients with candidemia. Those patients were more likely to receive echinocandins as initial treatment and were less severely ill and less likely infected with C. parapsilosis., Background: To date, azoles represent the only viable option for oral treatment of invasive Candida infections, while rates of azole resistance among non-albicans Candida spp. continue to increase. The objective of this sub-analysis of the European multicenter observational cohort study Candida III was to describe demographical and clinical characteristics of the cohort requiring prolonged hospitalization solely to complete intravenous (iv) antifungal treatment (AF Tx). Methods: Each participating hospital (number of eligible hospitals per country determined by population size) included the first ~ 10 blood culture proven adult candidemia cases occurring consecutively after July 1st, 2018, and treating physicians answered the question on whether hospital stay was prolonged only for completion of intravenous antifungal therapy. Descriptive analyses as well as binary logistic regression was used to assess for predictors of prolonged hospitalization solely to complete iv AF Tx. Findings: Hospital stay was prolonged solely for the completion of iv AF Tx in 16% (100/621) of candidemia cases by a median of 16 days (IQR 8 – 28). In the multivariable model, initial echinocandin treatment was a positive predictor for prolonged hospitalization to complete iv AF Tx (aOR 2.87, 95% CI 1.55 – 5.32, p < 0.001), while (i) neutropenia, (ii) intensive care unit admission, (iii) catheter related candidemia, (iv) total parenteral nutrition, and (v) C. parapsilosis as causative pathogen were found to be negative predictors (aOR 0.22 – 0.45; p < 0.03). Interpretation: Hospital stays were prolonged due to need of iv AF Tx in 16% of patients with candidemia. Those patients were more likely to receive echinocandins as initial treatment and were less severely ill and less likely infected with C. parapsilosis.

Published
2023

28. Anti-Toxoplasma IgG assays: What performances for what purpose? A systematic review

Author

Robert-Gangneux Florence and Guegan Hélène

Subjects
anti-toxoplasma igg, diagnosis, serology, toxoplasmosis, sensitivity, specificity, Infectious and parasitic diseases, RC109-216
Abstract

Chronic infection with Toxoplasma gondii is attested by the detection of specific anti-Toxoplasma IgG. A wide panel of serologic methods is currently marketed, and the most suitable method should be chosen according to the laboratory resources and the screened population. This systematic review of evaluation studies aimed at establishing an overview of the performances, i.e. sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of marketed anti-Toxoplasma IgG assays, and discussing their technical characteristics to guide further choice for routine diagnostic use. According to PRISMA guidelines, the search performed in PubMed and Web of Science databases recovered 826 studies, of which 17 were ultimately included. Twenty commercial anti-Toxoplasma IgG assays were evaluated, in comparison with an accepted reference method. Most of them were enzyme-immunoassays (EIAs, n = 12), followed by agglutination tests (n = 4), immunochromatographic tests (n = 3), and a Western-Blot assay (WB, n = 1). The mean sensitivity of IgG assays ranged from 89.7% to 100% for standard titers and from 13.4% to 99.2% for low IgG titers. A few studies pointed out the ability of some methods, especially WB to detect IgG early after primary infection. The specificity of IgG assays was generally high, ranging from 91.3% to 100%; and higher than 99% for most EIA assays. The PPV was not a discriminant indicator among methods, whereas significant disparities (87.5%–100%) were reported among NPVs, a key-parameter assessing the ability to definitively rule out a Toxoplasma infection in patients at-risk for opportunistic infections.

Published
2021
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33. Deciphering the Role of PIG1 and DHN-Melanin in Scedosporium apiospermum Conidia

Author

Guegan, Hélène, primary, Poirier, Wilfried, additional, Ravenel, Kevin, additional, Dion, Sarah, additional, Delabarre, Aymeric, additional, Desvillechabrol, Dimitri, additional, Pinson, Xavier, additional, Sergent, Odile, additional, Gallais, Isabelle, additional, Gangneux, Jean-Pierre, additional, Giraud, Sandrine, additional, and Gastebois, Amandine, additional

Published
2023
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34. Evaluation of the PneumoGenius® PCR assay for the diagnosis of Pneumocystis pneumonia and the detection of Pneumocystis dihydropteroate synthase mutations in respiratory samples.

Author

Guegan, Hélène, Roojee, Maël, Le Gal, Solène, Artus, Mathilde, Nevez, Gilles, Gangneux, Jean-Pierre, and Robert-Gangneux, Florence

Abstract

Pneumocystis pneumonia (PCP) is the most frequent fungal opportunistic infection defining AIDS in HIV-infected patients, and is of growing importance in HIV-negative patients. In this latter category of patients, the diagnosis mainly relies on real-time polymerase chain reaction (qPCR) detection of Pneumocystis jirovecii (Pj) on respiratory samples. The PneumoGenius® kit (PathoNostics) allows the simultaneous detection of Pj mitochondrial large subunit (mtLSU) and dihydropteroate synthase (DHPS) polymorphisms, which could be of interest to anticipate therapeutic failure. This study aimed at evaluating its clinical performance on 251 respiratory specimens (239 patients), (i) for P. jirovecii detection in clinical samples, and (ii) for DHPS polymorphisms detection in circulating strains. Patients were classified according to modified European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) criteria, as having proven PCP (n = 62), probable PCP (n = 87), Pneumocystis colonization (n = 37), and no PCP (n = 53). Compared with in-house qPCR, the sensitivity of PneumoGenius® assay for P. jirovecii detection reached 91.9% (182/198), the specificity was excellent (100%, 53/53) and the global concordance was 93.6% (235/253). A total of four diagnoses of proven/probable PCP were missed by the PneumoGenius® assay, reaching a 97.5% sensitivity (157/161) in this sub-group. The 12 other 'false–negative' results were obtained in patients diagnosed as colonized using the in-house PCR. DHPS genotyping was successful for 147/182 samples with PneumoGenius® and revealed dhps mutation in 8 samples, which were all confirmed by sequencing. In conclusion, PneumoGenius® assay missed the detection of low-burden PCP. This lower sensitivity for PCP diagnosis can be balanced by a higher specificity (P. jirovecii colonization less frequently detected) and the efficient detection of DHPS hot spot mutations. Lay Summary: The diagnosis of Pneumocystis pneumonia (PCP) relies on DNA detection of P. jirovecii in respiratory samples. In this study, we show that the commercial assay PneumoGenius® has a lower sensitivity than our in-house qPCR for PCP diagnosis, but provides accurate results for DHPS genotyping. [ABSTRACT FROM AUTHOR]

Published
2023
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35. La qualité de l’air intérieur des structures médico-sociales et libérales

Author

Baurès, Estelle, Baudet, Alexandre, Guegan, Hélène, Blanchard, Olivier, Guillaso, Monique, Le Cann, Pierre, Gangneux, Jean-Pierre, Florentin, Arnaud, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP), Adaptation, mesure et évaluation en santé. Approches interdisciplinaires (APEMAC), Université de Lorraine (UL), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Faculté de Médecine [Nancy], and CHU Pontchaillou [Rennes]

Subjects
Microbiologie environnementale, Développement durable, [SDE]Environmental Sciences, Etablissements médico-sociaux, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Composés organiques, Pollution, Qualité de l'air intérieur
Abstract

International audience; La qualité de l’air intérieur est à ce jour encore peu étudiée. Via le projet MEDIQAI, deux campagnes de prélèvements et mesures (été 2018 et hiver 2019) ont été menées à Rennes et Nancy, dans six structures libérales et quatre établissements médico-sociaux. Les concentrations médianes de composés chimiques les plus élevées ont été mesurées pour les alcools issus des activités de soins, et plus particulièrement des solutions hydroalcooliques et désinfectantes. Résultat : l’air intérieur analysé contient un mélange complexe de nombreux polluants, avec des concentrations plus élevées que dans l’air intérieur des centres hospitaliers.

Published
2022

36. Chromoblastomycosis Due to a Never-before-Seen Dematiaceous Fungus in a Kidney Transplant Patient

Author

Guegan, Hélène, Cailleaux, Marine, Le Gall, François, Robert-Gangneux, Florence, Gangneux, Jean-Pierre, Institut de recherche en santé, environnement et travail (Irset), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Santé et de la Recherche Médicale (INSERM)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université d'Angers (UA), École des Hautes Études en Santé Publique [EHESP] (EHESP), CHU Pontchaillou [Rennes], Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Chard-Hutchinson, Xavier

Subjects
[SDV] Life Sciences [q-bio], kidney transplant, Kirschsteiniotheliales, fumagoid cell, QH301-705.5, [SDV]Life Sciences [q-bio], Dothideomycetes, Case Report, Biology (General), chromoblastomycosis
Abstract

International audience; Chromoblastomycosis is a neglected fungal infection of the epidermis and subcutaneous tissue that predominates in tropical areas and results from the traumatic inoculation of environmental dematiaceous filamentous fungi. We describe the case of an immunosuppressed patient diagnosed with foot chromoblastomycosis due to an uncommon dematiaceous fungus. A 52-year-old Congolese kidney transplant woman presented with a painful lesion located on the foot. No trauma to the lower limbs was reported during the previous months. She lived in France and had not returned to the Congo over the previous eight years. Histology and mycological examination from skin biopsy revealed swollen dark filaments associated with dematiaceous muriform cells, pathognomonic of chromoblastomycosis. Cultures grew with dark pigmented colonies, yielding poor microscopic features. The phylogenetic analysis confirmed that the isolate was a member of Kirschsteiniotheliales (Dothideomycetes) and unrelated to the Chaetotyriales, of which most species commonly responsible for chromoblastomycosis belong. As there was no bone spreading, excision surgery of the entire lesion followed by liposomal amphotericin B therapy resulted in complete healing after six months. This original case illustrates the potential diversity of environmental dematiaceous fungi responsible for phaeohyphomycosis, especially chromoblastomycosis, and the need to send samples to mycology labs for appropriate diagnosis.

Published
2021

38. Proteomic Analysis of Humoral Immune Components in Bronchoalveolar Lavage of Patients Infected or Colonized by Aspergillus fumigatus

Author

Dellière, Sarah, Duchateau, Magalie, Wong, Sarah Sze Wah, Giai Gianetto, Quentin, Guegan, Hélène, Matondo, Mariette, Gangneux, Jean-Pierre, Aimanianda, Vishukumar, Mycologie moléculaire - Molecular Mycology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Parasitologie et Mycologie (Parasito-Myco - ST LOUIS PARIS), Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Plateforme de Protéomique / Proteomics platform, Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology (UTechS MSBio), Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This work was supported by ANR-FUNHYDRO (ANR-16S-CE110020-01) grant., ANR-16-CE11-0020,FUNHYDRO,Amyloïdes fonctionnels formés par les hydrophobines du pathogène fongique Aspergillus fumigatus(2016), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Giai Gianetto, Quentin, and Amyloïdes fonctionnels formés par les hydrophobines du pathogène fongique Aspergillus fumigatus - - FUNHYDRO2016 - ANR-16-CE11-0020 - AAPG2016 - VALID

Subjects
Male, Proteome, [SDV.IMM] Life Sciences [q-bio]/Immunology, Immunology, proteomics, humoral immunity, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], RNA, Ribosomal, 28S, Humans, bronchoalveolar lavage, aspergillosis, Protein Interaction Maps, skin and connective tissue diseases, complement system, Aged, Aspergillus fumigatus, RNA, Fungal, Complement System Proteins, Middle Aged, Immunity, Innate, Immunity, Humoral, Pulmonary Alveoli, Case-Control Studies, Perspective, Host-Pathogen Interactions, pentraxin-3, Cytokines, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, sense organs, Bronchoalveolar Lavage Fluid
Abstract

Humoral immune components have been individually studied in the context of interaction of host with Aspergillus fumigatus, a major airborne fungal pathogen. However, a global view of the multitude and complex nature of humoral immune components is needed to bring new insight into host-Aspergillus interaction. Therefore, we undertook comparative proteomic analysis of the bronchoalveolar lavage fluid collected from individuals infected or colonized with A. fumigatus versus controls, to identify those alveolar humoral components affected upon A. fumigatus infection. Complement proteins C1q, C8 beta-chain, factor-H, ficolin-1, ficolin-2, mannan binding lectin serine peptidase 2, pentraxin-3 and the surfactant protein-D were identified as the major humoral immune components affected by A. fumigatus infection and colonization. Based on this observation, we hypothesize that crosstalk between these humoral components is essential during host-Aspergillus interaction giving new specific leads to study for better understanding the pathogenesis. Furthermore, the affected humoral components could be potential diagnostic markers of A. fumigatus infection or colonization.

Published
2021

39. Influenza- and COVID-19-Associated Pulmonary Aspergillosis: Are the Pictures Different?

Author

Reizine, Florian, primary, Pinceaux, Kieran, additional, Lederlin, Mathieu, additional, Autier, Brice, additional, Guegan, Hélène, additional, Gacouin, Arnaud, additional, Luque-Paz, David, additional, Boglione-Kerrien, Christelle, additional, Bacle, Astrid, additional, Le Daré, Brendan, additional, Launey, Yoann, additional, Lesouhaitier, Mathieu, additional, Painvin, Benoit, additional, Camus, Christophe, additional, Mansour, Alexandre, additional, Robert-Gangneux, Florence, additional, Belaz, Sorya, additional, Le Tulzo, Yves, additional, Tadié, Jean-Marc, additional, Maamar, Adel, additional, and Gangneux, Jean-Pierre, additional

Published
2021
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40. Is the COVID-19 Pandemic a Good Time to Include Aspergillus Molecular Detection to Categorize Aspergillosis in ICU Patients? A Monocentric Experience

Author

Gangneux, J-P, Reizine, Florian, Guegan, Hélène, Pinceaux, Kieran, Le Balch, Pierre, Prat, Emilie, Pelletier, Romain, Belaz, Sorya, Le Souhaitier, Mathieu, Le Tulzo, Yves, Seguin, Philippe, Lederlin, Mathieu, Tadié, Jean-Marc, Robert-Gangneux, Florence, CHU Pontchaillou [Rennes], Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )

Subjects
invasive aspergillosis, Sars-CoV-2, COVID-19, Article, probable, Aspergillus, PCR, putative, classification, lcsh:Biology (General), [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, galactomannan, ICU, lcsh:QH301-705.5
Abstract

(1) Background: The diagnosis of invasive aspergillosis (IA) in an intensive care unit (ICU)remains a challenge and the COVID-19 epidemic makes it even harder. Here, we evaluatedAspergillus PCR input to help classifying IA in SARS-CoV-2-infected patients. (2) Methods: 45COVID-19 patients were prospectively monitored twice weekly for Aspergillus markers and anti-Aspergillus serology. We evaluated the concordance between (&Iota, ) Aspergillus PCR and culture inrespiratory samples, and (&Iota, &Iota, ) blood PCR and serum galactomannan. Patients were classified asputative/proven/colonized using AspICU algorithm and two other methods. (3) Results: Theconcordance of techniques applied on respiratory and blood samples was moderate (kappa = 0.58and kappa = 0.63, respectively), with a higher sensitivity of PCR. According to AspICU, 9/45 patientswere classified as putative IA. When incorporating PCR results, 15 were putative IA because theymet all criteria, probably with a lack of specificity in the context of COVID-19. Using a modifiedAspICU algorithm, eight patients were classified as colonized and seven as putative IA. (4)Conclusion: An appreciation of the fungal burden using PCR and Aspergillus serology was addedto propose a modified AspICU algorithm. This proof of concept seemed relevant, as it was inagreement with the outcome of patients, but will need validation in larger cohorts.

Published
2020

42. Maternal Anti- Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate

Author

Guegan, Hélène, primary, Stajner, Tijana, additional, Bobic, Branko, additional, Press, Cindy, additional, Olariu, Rares T., additional, Olson, Kjerstie, additional, Srbljanovic, Jelena, additional, Montoya, Jose G., additional, Djurković-Djaković, Olgica, additional, and Robert-Gangneux, Florence, additional

Published
2021
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43. High Prevalence of Fungal Infections in Mechanically Ventilated COVID-19 Patients in the ICU: The French Multicenter MYCOVID Study

Author

Gangneux, Jean Pierre, primary, Dannaoui, Eric, additional, Fekkar, Arnaud, additional, Luyt, Charles Edouard, additional, Botterel, Francoise, additional, de Prost, Nicolas, additional, Tadie, Jean Marc, additional, Reizine, Florian, additional, Houze, Sandrine, additional, Timsit, Jean-François, additional, Iriart, Xavier, additional, Riu-Poulenc, Béatrice, additional, Sendid, Boualem, additional, Nseir, Saad, additional, Persat, Florence, additional, Wallet, Florent, additional, Le Pape, Patrice, additional, Canet, Emmanuel, additional, Novara, Ana, additional, Manai, Melek, additional, Cateau, Estelle, additional, Thille, Arnaud W., additional, Brun, Sophie, additional, Cohen, Yves, additional, Alanio, Alexandre, additional, Megarbane, Bruno, additional, Cornet, Muriel, additional, Terzi, Nicolas, additional, Lamhaut, Lionel, additional, Sabourin, Estelle, additional, Desoubeaux, Guillaume, additional, Ehrmann, Stephan, additional, Hennequin, Christophe, additional, Voiriot, Guillaume, additional, Nevez, Gilles, additional, Aubron, Cécile, additional, Letscher-Bru, Valérie, additional, Meziani, Ferhat, additional, Blaize, Marion, additional, Mayaux, Julien, additional, Monsel, Antoine, additional, Boquel, Frederique, additional, Robert-Gangneux, Florence, additional, Le Tulzo, Yves, additional, Seguin, Philippe, additional, Guegan, Hélène, additional, Autier, Brice, additional, Lesouhaitier, Mathieu, additional, Pelletier, Romain, additional, Belaz, Sorya, additional, Bonnal, Christine, additional, Berry, Antoine, additional, Leroy, Jordan, additional, François, Nadine, additional, Richard, Jean-Christophe, additional, Paulus, Sylvie, additional, Argaud, Laurent, additional, Dupont, Damien, additional, Menotti, Jean, additional, Morio, Florent, additional, Soulié, Marie, additional, Schwebel, Carole, additional, Garnaud, Cécile, additional, Guitard, Juliette, additional, Le Gal, Solène, additional, Quinio, Dorothée, additional, Morcet, Jeff, additional, Laviolle, Bruno, additional, Zahar, Jean-Ralph, additional, and Bougnoux, Marie-Elisabeth, additional

Published
2021
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44. Differential Interactions of Serum and Bronchoalveolar Lavage Fluid Complement Proteins with Conidia of Airborne Fungal Pathogen Aspergillus fumigatus

Author

Wong, Sarah Sze Wah, primary, Daniel, Irene, additional, Gangneux, Jean-Pierre, additional, Jayapal, Jeya Maheshwari, additional, Guegan, Hélène, additional, Dellière, Sarah, additional, Lalitha, Prajna, additional, Shende, Rajashri, additional, Madan, Taruna, additional, Bayry, Jagadeesh, additional, Guijarro, J. Iñaki, additional, Kuppamuthu, Dharmalingam, additional, and Aimanianda, Vishukumar, additional

Published
2020
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46. Aspergillus fumigatus Infection in Humans With STAT3-Deficiency Is Associated With Defective Interferon-Gamma and Th17 Responses

Author

Danion, François, primary, Aimanianda, Vishukumar, additional, Bayry, Jagadeesh, additional, Duréault, Amélie, additional, Wong, Sarah Sze Wah, additional, Bougnoux, Marie-Elisabeth, additional, Tcherakian, Colas, additional, Alyanakian, Marie-Alexandra, additional, Guegan, Hélène, additional, Puel, Anne, additional, Picard, Capucine, additional, Lortholary, Olivier, additional, Lanternier, Fanny, additional, and Latgé, Jean-Paul, additional

Published
2020
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48. Real-time PCR for diagnosis of imported schistosomiasis

Author

Guegan, Hélène, Fillaux, Judith, Charpentier, Elena, Robert-gangneux, Florence, Chauvin, Pamela, Guemas, Emilie, Boissier, Jérôme, Valentin, Alexis, Cassaing, Sophie, Gangneux, Jean-pierre, Berry, Antoine, Iriart, Xavier, Guegan, Hélène, Fillaux, Judith, Charpentier, Elena, Robert-gangneux, Florence, Chauvin, Pamela, Guemas, Emilie, Boissier, Jérôme, Valentin, Alexis, Cassaing, Sophie, Gangneux, Jean-pierre, Berry, Antoine, and Iriart, Xavier

Abstract

Background The diagnosis of schistosomiasis currently relies on microscopic detection of schistosome eggs in stool or urine samples and serological assays. The poor sensitivity of standard microscopic procedures performed in routine laboratories, makes molecular detection methods of increasing interest. The aim of the study was to evaluate two in-house real-time Schistosoma PCRs, targeting respectively S. mansoni [Sm] and S. haematobium [Sh] in excreta, biopsies and sera as potential tools to diagnose active infections and to monitor treatment efficacy. Methods Schistosoma PCRs were performed on 412 samples (124 urine, 86 stools, 8 biopsies, 194 sera) from patients with suspected schistosomiasis, before anti-parasitic treatment. Results were compared to microscopic examination and serological assays (enzyme-linked immunosorbent assay (ELISA), indirect haemagglutination (HA) and Western Blot (WB) assay). Results Compared to microscopy, PCRs significantly increased the sensitivity of diagnosis, from 4% to 10.5% and from 33.7% to 48.8%, for Sh in urine and Sm in stools, respectively. The overall sensitivity of PCR on serum samples was 72.7% and reached 94.1% in patients with positive excreta (microscopy). The specificity of serum PCR was 98.9%. After treatment, serum PCR positivity rates slowly declined from 93.8% at day 30 to 8.3% at day 360, whereas antibody detection remained positive after 1 year. Conclusion Schistosoma PCRs clearly outperform standard microscopy on stools and urine and could be part of reference methods combined with WB-based serology, which remains a gold standard for initial diagnosis. When serological assays are positive and microscopy is negative, serum PCRs provide species information to guide further clinical exploration. Biomarkers such as DNA and antibodies are of limited relevance for early treatment monitoring but serum PCR could be useful when performed at least 1 year after treatment to help confirm a cured infection. Author summary Sc

Published
2019
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50. Real-time PCR for diagnosis of imported schistosomiasis

Author

Guegan, Hélène, primary, Fillaux, Judith, additional, Charpentier, Eléna, additional, Robert-Gangneux, Florence, additional, Chauvin, Pamela, additional, Guemas, Emilie, additional, Boissier, Jérôme, additional, Valentin, Alexis, additional, Cassaing, Sophie, additional, Gangneux, Jean-Pierre, additional, Berry, Antoine, additional, and Iriart, Xavier, additional

Published
2019
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