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24 results on '"Guckenberger, David J."'

3. Supplementary Methods, Supplementary Figures S1, S2, Supplementary Tables S1-S3 from Integrated Analysis of Multiple Biomarkers from Circulating Tumor Cells Enabled by Exclusion-Based Analyte Isolation

Author

Sperger, Jamie M., primary, Strotman, Lindsay N., primary, Welsh, Allison, primary, Casavant, Benjamin P., primary, Chalmers, Zachery, primary, Horn, Sacha, primary, Heninger, Erika, primary, Thiede, Stephanie M., primary, Tokar, Jacob, primary, Gibbs, Benjamin K., primary, Guckenberger, David J., primary, Carmichael, Lakeesha, primary, Dehm, Scott M., primary, Stephens, Philip J., primary, Beebe, David J., primary, Berry, Scott M., primary, and Lang, Joshua M., primary

Published
2023
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6. Supplementary_Information – Supplemental material for Induced Pluripotent Stem Cells on a Chip: A Self-Contained, Accessible, Pipette-less iPSC Culturing and Differentiation Kit

Author

McMinn, Patrick, Guckenberger, David J., and Beebe, David J.

Subjects
FOS: Clinical medicine, education, humanities, 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified
Abstract

Supplemental material, Supplementary_Information for Induced Pluripotent Stem Cells on a Chip: A Self-Contained, Accessible, Pipette-less iPSC Culturing and Differentiation Kit by Patrick McMinn, David J. Guckenberger and David J. Beebe in SLAS Technology

Published
2020
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8. Versatile exclusion-based sample preparation platform for integrated rare cell isolation and analyte extraction

Author

Pezzi, Hannah M., primary, Guckenberger, David J., additional, Schehr, Jennifer L., additional, Rothbauer, Jacob, additional, Stahlfeld, Charlotte, additional, Singh, Anupama, additional, Horn, Sacha, additional, Schultz, Zachery D., additional, Bade, Rory M., additional, Sperger, Jamie M., additional, Berry, Scott M., additional, Lang, Joshua M., additional, and Beebe, David J., additional

Published
2018
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10. Integrated Analysis of Multiple Biomarkers from Circulating Tumor Cells Enabled by Exclusion-Based Analyte Isolation

Author

Sperger, Jamie M., primary, Strotman, Lindsay N., additional, Welsh, Allison, additional, Casavant, Benjamin P., additional, Chalmers, Zachery, additional, Horn, Sacha, additional, Heninger, Erika, additional, Thiede, Stephanie M., additional, Tokar, Jacob, additional, Gibbs, Benjamin K., additional, Guckenberger, David J., additional, Carmichael, Lakeesha, additional, Dehm, Scott M., additional, Stephens, Philip J., additional, Beebe, David J., additional, Berry, Scott M., additional, and Lang, Joshua M., additional

Published
2017
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13. High Specificity in Circulating Tumor Cell Identification Is Required for Accurate Evaluation of Programmed Death-Ligand 1

Author

Schehr, Jennifer L., primary, Schultz, Zachery D., additional, Warrick, Jay W., additional, Guckenberger, David J., additional, Pezzi, Hannah M., additional, Sperger, Jamie M., additional, Heninger, Erika, additional, Saeed, Anwaar, additional, Leal, Ticiana, additional, Mattox, Kara, additional, Traynor, Anne M., additional, Campbell, Toby C., additional, Berry, Scott M., additional, Beebe, David J., additional, and Lang, Joshua M., additional

Published
2016
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16. Using Exclusion-Based Sample Preparation (ESP) to Reduce Viral Load Assay Cost

Author

Berry, Scott M., primary, Pezzi, Hannah M., additional, Williams, Eram D., additional, Loeb, Jennifer M., additional, Guckenberger, David J., additional, Lavanway, Alex J., additional, Puchalski, Alice A., additional, Kityo, Cissy M., additional, Mugyenyi, Peter N., additional, Graziano, Franklin M., additional, and Beebe, David J., additional

Published
2015
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23. Interrogating Bronchoalveolar Lavage Samples via Exclusion-Based Analyte Extraction

Author

Ho, Dean, Zarrinpar, Ali, Tokar, Jacob J., Warrick, Jay W., Guckenberger, David J., Sperger, Jamie M., Lang, Joshua M., Ferguson, J. Scott, and Beebe, David J.

Abstract

Although average survival rates for lung cancer have improved, earlier and better diagnosis remains a priority. One promising approach to assisting earlier and safer diagnosis of lung lesions is bronchoalveolar lavage (BAL), which provides a sample of lung tissue as well as proteins and immune cells from the vicinity of the lesion, yet diagnostic sensitivity remains a challenge. Reproducible isolation of lung epithelia and multianalyte extraction have the potential to improve diagnostic sensitivity and provide new information for developing personalized therapeutic approaches. We present the use of a recently developed exclusion-based, solid-phase-extraction technique called SLIDE (Sliding Lid for Immobilized Droplet Extraction) to facilitate analysis of BAL samples. We developed a SLIDE protocol for lung epithelial cell extraction and biomarker staining of patient BALs, testing both EpCAM and Trop2 as capture antigens. We characterized captured cells using TTF1 and p40 as immunostaining biomarkers of adenocarcinoma and squamous cell carcinoma, respectively. We achieved up to 90% (EpCAM) and 84% (Trop2) extraction efficiency of representative tumor cell lines. We then used the platform to process two patient BAL samples in parallel within the same sample plate to demonstrate feasibility and observed that Trop2-based extraction potentially extracts more target cells than EpCAM-based extraction.

Published
2017
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