Search

A ferritin mutant A1C with 24 free SH groups on its outer surface was prepared, which was successfully cross-linked to hen eggshell membrane outer layer (ESM-OL) by a disulfide linkage as characterized by SEM and TEM. Generally, this ferritin conjugated ESM-OL (Ft-ESM-OL) exhibited a significantly higher activity of heavy metal ion uptake as compared to ESM-OL alone under the same experimental conditions. For example, the adsorption capacity of Ft-ESM-OL for U6+ and Fe2+ were 21.16% and 18.4% higher than that of natural ESM-OL, respectively. On the other hand, the recovery capacity of Fe2+ by Ft-ESM-OL was 8.11 mg/g ESM, a value 67.9% higher than that of natural ESM-OL. Thus, this newly ferritin-modified ESM-OL greatly improved the adsorption and recovery efficiency of heavy metal ion as compared to ESM-OL alone.

To investigate the autolysis pattern and activation of metacaspase in higher plants, the biochemical characteristics of purified recombinant type II metacaspase (LeMCA1) from tomato were explored. Western blotting analysis indicated that four cleaved bands were formed; two N-terminal fragments and two C-terminal fragments. N-terminal sequencing confirmed that LeMCA1 cleaves at Lys223 and Arg332. Site mutants indicated that catalytic Cys139, cleaved Lys223, Arg332 and predicted calcium binding Asp116/Asp117 are the key residues that are responsible for its Ca2+ and pH dependent activation. The cleavage of the full-size fragment seemed crucial for the activation of LeMCA1 in vitro.

Porous SiC foamed ceramics used raw material of SiC were prepared by adjusting slurry viscosity at 1400°C. Formed technology was dipping organic precursors. The result showed that the section of the sample presents three-dimensional net structure, wall of hole thickness is proper, the distribution of the hole are homogeneous, and have high porosity, apertures are small than 30 μm, the main crystal appearance is SiC. The strength of samples is been improved by dealing organic precursors. The slurry on the ribs is been increased.

The synthesis and characterization of a Zn(II) complex with 5-ethyl-2-amino-1,3,4-thiadiazole (eatz) are described. The crystal structure of [Zn(eatz)2(OAc)2] was determined by X-ray diffraction. The crystal belongs to the monoclinic system and the structural unit is a distorted tetrahedron. In this complex, eatz acts as a monodentate ligand coordinating through the thiadiazole N atom to Zn(II), and the crystal structure is mainly stabilized by both intra- and intermolecular hydrogen bonds. Thermal analysis reveals that the complex [Zn(eatz)2(OAc)2] decomposes in three transitions to give Zn(CN)2 as the end product at 650 °C in N2 atmosphere. The thermal decomposition kinetic analysis of the title complex indicates that the first transition is a double-step reaction with the main reaction being an nth auto-catalysis one. A biological activity study shows that both the ligand and the complex have good antimicrobial activities against Staphylococcus aureus.

The deferrization mechanism of apoferritin was established, and the spectra variation of apoferritin was compared with that of holoferritin. Sodium hyposulfite is a strong reducing agent, therefore, was applied to deoxidize holoferritin to release iron ion, and connection of iron of buffer was measured by the 2,2-dipyridyl. Apoferritin was detected by ICP-MS. Holoferritin was found to have no absorption compared with apoferritin by UV analysis, and have no fluorescence emission spectra in contrast with apoferritin by fluorescence analysis.

A double labial-arch retaining appliance to retain the treatment effective of Class III osteal malocclusion were introduced and used to retain 60 patients with Class III osteal malocclusion. At the same time, 60 patients with Class III osteal malocclusion were retained with conventional Hawley retainer as the control group. The results indicated that the double labial-arch retaining appliance has good effect to the retain of Class III osteal malocclusion.

The needs of Olympic spectators in different stages of the walking chains are thought in a whole, when they being on the way between traffic facilities and venue and in the entrance/exit spots. Pedestrian characteristics are analyzed in ingress and egress, based on observations in related area. According to the simultaneous analysis on pedestrian traffic characteristics and behaviors, pedestrian management blueprint of Olympic venue will be drawn out under related condition, which is the basement of pedestrian simulation. The pedestrian simulation model should be the valid method to evaluate the results of cases, key criteria being traffic flow characters. The method of pedestrian simulation is practical on evaluation on the different project of construction, management and operation.