5 results on '"Guang-Fei Xu"'
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2. Resveratrol protects BV2 mouse microglial cells against LPS-induced inflammatory injury by altering the miR-146a-5p/TRAF6/NF-κB axis
- Author
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Ying Lu, Yu-Ting Ge, An-Qi Zhong, and Guang-Fei Xu
- Subjects
Lipopolysaccharides ,0301 basic medicine ,Lipopolysaccharide ,Cell Survival ,Immunology ,Anti-Inflammatory Agents ,Cell Culture Techniques ,Resveratrol ,Toxicology ,Cell Line ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Western blot ,medicine ,Animals ,Immunology and Allergy ,MTT assay ,Viability assay ,TNF Receptor-Associated Factor 6 ,Pharmacology ,Dose-Response Relationship, Drug ,medicine.diagnostic_test ,NF-kappa B ,NF-κB ,General Medicine ,Transfection ,Molecular biology ,Up-Regulation ,MicroRNAs ,030104 developmental biology ,TNF receptor associated factor ,chemistry ,030220 oncology & carcinogenesis ,Cytokines ,Microglia ,Signal Transduction - Abstract
Objective: To investigate the role of miR-146a-5p in the effects of resveratrol (RSV) on inflammatory response in BV2 mouse microglial cells. Materials and methods: BV2 cells were pretreated by RSV and stimulated with lipopolysaccharide (LPS). Cell Viability was checked using a MTT assay. Real-Time PCR was performed to detect the levels of pro-inflammatory cytokines (tumor necrosisfactor-α－TNF-α, interleukin-1β－IL-1β and interleukin-6 － IL-6) and miR-146a-5p expression. Western blot was used to analyze the protein expression of TNF receptor associated factor 6 (TRAF6) and phospho-nuclear factor kappa B (pNF-κB). Gain-of-function and loss-of-function analysis of miR-146a-5p was performed using transfection of miR-146a-5p mimic and miR-146a-5p inhibitor, respectively. Results: Pretreatment with RSV significantly and dose dependently inhibited LPS-induced production of TNF-α, IL-1β and IL-6 in BV2 cells. MiR-146a-5p was significantly upregulated after LPS treatment, and further increased in RSV and LPS-co-treated cells. MiR-146a-5p overexpression via miR-146a-5p mimic transfection downregulated the mRNA level of TNF-α, IL-1β and IL-6, as well as abrogated the protein expression of TRAF6 and pNF-κB in BV2 cells exposed to LPS. More importantly, the reducion of TNF-α, IL-1β and IL-6 level by RSV were reversed by miR-146a-5p silence via miR-146a-5p inhibitor transfection. Furthermore, silencing miR-146a-5p attenuated the inhibitory effect of RSV on the TRAF6/NF-κB pathway which was activated after induction with LPS. Conclusions: RSV can suppress LPS-induced inflammatory injury via modulating the miR-146a-5p/TRAF6/NF-κB axis in BV2 mouse microglial cells.
- Published
- 2019
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3. The Preparation of Fe3O4 Magnetic Nanometer Particle by Co-Precipitation Method
- Author
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Xiao Chun Ma, Lei Hao Cui, and Guang Fei Xu
- Subjects
Diffraction ,Materials science ,Chemical engineering ,Active agent ,Transmission electron microscopy ,Coprecipitation ,Nanometer particle ,Nanotechnology ,General Medicine ,Dispersion (chemistry) - Abstract
In this paper, the Fe3O4 magnetic nanometer particle was prepared by co-precipitation method. At the same time, the samples were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The results show that the surface active agent (PEG4000) can be good for the dispersion performance of Fe3O4 magnetic nanometer particle; and the temperature of 80°C is the appropriate drying temperature to prepare the Fe3O4 magnetic nanometer particle.
- Published
- 2012
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4. Failure Analysis of Hydrogen Corrosion of the Lens Gasket
- Author
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Xiao Chun Ma and Guang Fei Xu
- Subjects
Materials science ,Hydrogen ,Gasket ,chemistry.chemical_element ,Lens (geology) ,General Medicine ,Flange ,Intergranular corrosion ,Microstructure ,Corrosion ,chemistry ,Forensic engineering ,Composite material ,Anaerobic corrosion - Abstract
The 10MoWVNb steel lens gaskets are widely used for the flange linking of high pressure tube in the synthetic ammonia system in chemical fertilizer plant. The gasket worked in H2-N2-NH3 environment at the temperature of 300°C. It often fails because of hydrogen corrosion. The microstructure and corrosion characteristic of the lens gasket were studied by optical microscope. The results showed that the hydrogen blister appeared preferentially at the interface of inclusions. The corrosion depth was 0.65 millimeter. There were little intergranular crack on inner surface.
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- 2010
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5. Association of alcohol consumption and components of metabolic syndrome among people in rural China.
- Author
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Jing Xiao, Jian-Ping Huang, Guang-Fei Xu, De-Xi Chen, Gui-Yun Wu, Min Zhang, Yi Shen, and Hui Cai
- Subjects
BLOOD sugar analysis ,SMOKING ,ALCOHOLIC beverages ,ANALYSIS of variance ,BLOOD pressure measurement ,CHI-squared test ,COMPARATIVE studies ,CONFIDENCE intervals ,ALCOHOL drinking ,HIGH density lipoproteins ,LONGITUDINAL method ,NUTRITIONAL assessment ,PROBABILITY theory ,RESEARCH funding ,RURAL conditions ,SEX distribution ,STATISTICS ,TRIGLYCERIDES ,WINES ,LOGISTIC regression analysis ,DATA analysis ,SOCIOECONOMIC factors ,STATISTICAL significance ,METABOLIC syndrome ,BODY mass index ,CROSS-sectional method ,PHYSICAL activity ,DATA analysis software ,WAIST circumference ,DESCRIPTIVE statistics ,ODDS ratio - Abstract
Background: Accumulative evidence in the literature suggests alcohol consumption is a protective factor of the metabolic syndrome (MS). However, few studies investigated the relationship between alcohol consumption and components of MS. We examined association of several types of alcoholic beverage with components of MS among people in rural China. Methods: In the Nantong Metabolic Syndrome Study (NMSS), a cross-sectional study, a total of 20,502 participants, including 13,505 women and 6,997 men aged 18-74 years, were recruited between 2007 and 2008 in Nantong, China. Socio-economic status, dietary intake, physical exercise, alcoholic beverage consumption, and smoking status information were obtained, and triglycerides (TG), high-density lipoprtein cholesterol (HDL-c), blood pressure (BP) and blood glucose level were examined for all participants. Logistic regression model and the restricted cubic spline approach were used to analyze the associations between alcoholic beverage consumption and MS components. Results: The MS prevalence was 21.1% in the whole population, which was significantly low among drinkers (20.6%), compared with non-drinkers (23.6%) in women, and was comparable in men (16.4% versus 17.1%). High HDL-c level was observed among drinkers, compared with non-drinkers in both men and women. Low TG level and Systolic BP (SBP) were found only among rice wine drinkers in women, and high waist circumference, high TG and BP were found among beer and liquor drinkers in men. Furthermore, we found that the highest quartile of rice wine drink in women may decrease 24% risk of high TG, 30% risk of low HDL-c and 43% risk of high glucose among MS components cases respectively, compared with non-drinkers (p for trend <0.01 for those three components). While compared non-drinkers among men, the highest quartile of liquor drink may increase 32% risk of high SBP, 55% risk of high Diastolic BP (DBP) and 34% risk of abdominal obesity among MS components cases respectively, but decrease 45% risk of low HDL-c (p for trend <0.05 for those four components). Conclusion: Our data suggested that all alcoholic beverages increased HDL-c level. Rice wine decreased both TG level and blood glucose in women only and it could be one of healthy alcoholic beverages in MS prevention in Chinese women. While excessive liquor consumption increased BP and waist circumference level and it may lead to hypertension and central obesity in Chinese men. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
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