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204 results on '"Guan, Wenqiang"'

2. Effect of Vacuum Precooling on the Lignification and Related Enzyme Activities of Tricholoma matsutake

Author

ZHAO Zhuoran, WANG Fengling, WANG Xuan, GUAN Wenqiang, HU Xin, LIU Zhenguo, YAO Gang, LU Peng

Subjects
vacuum precooling, lignification, tricholoma matsutake, preservation, Food processing and manufacture, TP368-456
Abstract

In order to investigate the effect of vacuum precooling treatment on the lignification of Tricholoma matsutake during storage, the changes in the hardness of cap and stalk, pulp firmness, lignin, chitin and cellulose contents, and the activities of cellulase (CX), phenylalanine ammonia-lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) were studied during storage. The results showed that compared with the control group, vacuum precooling treatment could significantly inhibit the decline in the hardness of cap and stalk and pulp firmness, effectively inhibit the degradation of chitin and the increase of CX activity in the cell wall, maintain a higher cellulose content, and delay the accumulation of lignin. In addition, it could decrease the peak PAL activity of cap and stalk by 8.2% and 2.9%, the peak POD activity by 10% and 3.7%, and the peak PPO activity by 23.1% and 5.2%, respectively. In addition, compared with the cap, the peak time of lignification in the stalk was earlier, and lignification phenomenon was more likely to occur in the stalk. Therefore, vacuum precooling treatment can delay the lignification process and maintain the quality of T. matsutake.

Published
2024
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6. Purification and Enzymatic Properties of Soluble and Membrane-Bound Polyphenol Oxidase from Agaricus bisporus

Author

HE Xingxing, LEI Jing, GUAN Wenqiang

Subjects
agaricus bisporus, soluble polyphenol oxidase, membrane-bound polyphenol oxidase, purification, Food processing and manufacture, TP368-456
Abstract

An extraction and purification method for soluble polyphenol oxidase (sPPO) and membrane-bound polyphenol oxidases (mPPO) from Agaricus bisporus was developed involving temperature-induced phase partitioning, fractional precipitation with ammonium sulfate, and DEAE anion exchange chromatography. The purity, molecular mass and enzymatic reaction kinetics of purified PPO were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Native-PAGE and Michaelis-Menten equation. The specific activities of sPPO and mPPO toward catechol were 6 912.88 and 19 092.94 U/mg, and increased by 12.20 and 10.86 times, respectively, compared with that of crude PPO. The results of enzymatic reaction kinetics showed that the catalytic activities of sPPO and mPPO toward different substrates were greatly different and were higher toward catechol. mPPO had a stronger affinity to the substrate catechol and a higher reaction rate than sPPO. The optimum pH and temperature for both PPO enzymes were 6.8 and 30 ℃, respectively. This study may lay a foundation for future research on mass spectrometry identification and enzymatic properties two forms of PPOs from A. bisporu and inhibition of enzymatic browning caused by PPO.

Published
2023
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19. Enhancement of quality and self‐defense capacity of Agaricus bisporus by UV‐C treatment.

Author

He, Xingxing, Zhong, Jingwei, Wei, Ruhui, Li, Huijuan, Li, Jing, Ren, Yanlin, Zhai, Xinyu, Hu, Wenwen, and Guan, Wenqiang

Subjects
CULTIVATED mushroom, POLYPHENOL oxidase, PHENYLALANINE ammonia lyase, SELF-defense, ENZYMATIC browning, SUPEROXIDE dismutase
Abstract

BACKGROUND: Agaricus bisporus with a completely white appearance is popular with consumers. However, A. bisporus is susceptible to senescence and spoilage, which results in browning and oxidative tissue damage, with limited shelf life. This study investigated the effects of shortwave ultraviolet (UV‐C, 1.0 kJ m−2) irradiation on quality features, enzymatic browning, and defense properties of A. bisporus stored at 4 °C for 21 days. RESULTS: After storage, UV‐C irradiated A. bisporus had increased quality of sensory, color and hardness characteristics; it exhibited higher content of total phenolic, brown melanin precursors, including γ‐glutaminyl‐4‐hydroxybenzene, γ‐glutaminyl‐3,4‐dihydroxybenzene and tyrosine, lower accumulation of malondiadehyde, hydrogen peroxide and superoxide radical, and maintained membrane integrity in comparison to control samples. Besides, A. bisporus treated by UV‐C showed lower degree of browning and higher level of self‐defense capacity, which may be ascribed to the enhancement in activities of superoxide dismutase, catalase, phenylalanine ammonia lyase, chitinase and β‐1,3‐glucanase activity, while inhibiting polyphenol oxidase activity during storage. CONCLUSION: These results indicate that the higher self‐defense capacity with UV‐C treatment might be the mechanism associated with the delay of senescence in Agaricus bisporus. Therefore, UV‐C treatment is suggested as a potential practical application in mushrooms. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Published
2024
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23. Combined effects of paper‐containing 1‐methylcyclopropene with nitrogen‐modified atmosphere packaging in the logistics process of e‐commerce on fruit quality of plum (Prunus salicina cv. ‘Qingcuili’) during shelf life

Author

Zhang, Qianqian, primary, Guan, Wenqiang, additional, Xiong, Jinliang, additional, Lin, Hengxun, additional, Wang, Xueqing, additional, Huang, Ming, additional, Tan, Junjie, additional, Lei, Guisheng, additional, Luo, Qingyi, additional, and Zhao, Qian, additional

Published
2023
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38. UV‐C treatment inhibits browning, inactivates Pseudomonas tolaasii and reduces associated chemical and enzymatic changes of button mushrooms.

Author

Wang, Xueqing, He, Xingxing, Wu, Xinling, Fan, Xuetong, Wang, Fengling, Lin, Qiong, Guan, Wenqiang, and Zhang, Na

Subjects
POLYPHENOL oxidase, MUSHROOMS, PSEUDOMONAS diseases, PSEUDOMONAS, CHEMICAL industry
Abstract

BACKGROUND Button mushrooms with completely white appearance are popular with consumers. However, button mushrooms are susceptible to infection with Pseudomonas tolaasii, which results in browning. This study evaluates the effects of ultraviolet‐C (UV‐C) treatment on the inactivation of P. tolaasii in vitro and in vivo and on the physiological and chemical changes of button mushrooms during storage for 21 days at 4 °C. RESULTS: UV‐C doses of 0.5 to 9.0 kJ m−2 resulted in 3.91–6.26 log CFU mL−1 reduction of P. tolaasii populations in vitro, and UV‐C treatment reduced P. tolaasii populations inoculated on mushroom cap surfaces and browning severity. Moreover, P. tolaasii increased polyphenol oxidase (PPO) activity, and decreased phenylalanine ammonia‐lyase (PAL) activity, the accumulation of phenolics and contents of brown melanin precursors, including γ‐l‐glutaminyl‐4‐hydroxybenzene (GHB), γ‐l‐glutaminyl‐3,4‐dihydroxybenzene (GDHB), and tyrosine in button mushrooms. UV‐C treatment was found to reduce the negative changes due to P. tolaasii infection. CONCLUSION: These results indicated that the application of UV‐C treatment inhibited browning, inactivated P. tolaasii and reduced P. tolaasii – associated chemical and enzymatic changes of button mushrooms. © 2021 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Published
2022
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45. Effects of Siraitia grosvenoriiextracts on high fat diet-induced obese mice：a comparison with artificial sweetener aspartame

Author

Lü, Ke, Song, Xiaowan, Zhang, Peng, Zhao, Wei, Zhang, Ning, Yang, Fan, Guan, Wenqiang, Liu, Jianfu, Huang, He, Ho, Chi-Tang, Di, Rong, and Zhao, Hui

Abstract

Long-term artificial sweetener intake is linked to increased risk of obesity. In the present study, supplement of natural sweetener from Siraitia grosvenorii(SG) (or Momordica grosvenorii) fruit, compared with the artificial sweetener aspartame (ASM), was evaluated for anti-obesity effects on mice fed with high fat diet (HFD). We found that, in contrary to ASM, SG extracts prevented body weight gain, the insulin resistance and fat mass accumulation in HFD mice. SG extracts treatment inhibited the infiltration of inflammatory macrophages and lowered the levels of the fat inflammatory cytokines (leptin, macrophage chemoattractant protein 1 (MCP-1) and tumor necrosis factor-α (TNF-α)) in adipose tissues. In addition, SG extracts supplement counteracted the remodeling of gut microbiota resulted from HFD. However, ASM supplement aggravated the HFD-induced obese performances, fat inflammation and dysregulation of gut microbiota. Taken together, our results indicate that supplement of SG extracts may represent a promising alternation of artificial sweeteners in preventing metabolic diseases.

Published
2022
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48. The effects of fungal lipase-treated milk lipids on bread making

Author

Huang, Zhiguang, primary, Brennan, Charles Stephen, additional, Zheng, Haotian, additional, Mohan, Maneesha S., additional, Stipkovits, Letitia, additional, Liu, Wenjun, additional, Kulasiri, Don, additional, Guan, Wenqiang, additional, Zhao, Hui, additional, and Liu, Jianfu, additional

Published
2020
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