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As important genetic germ plasm resources and new potential cells for regenerative medicine, animal stem cells are broadly researched and utilized. In this study, Holstein bovine umbilical cord mesenchymal stem cells (UCMSCs) were isolated and investigated, and their multi-directional differentiation potential etc. biological characteristics were researched. The cells were isolated from 3-month-old fetal umbilical cords of Holstein cows using type IV collagenase, cultured in vitro. The obtained cells showed a typical "S" shape growth curve, and the population doubling time gradually decreased with the increase of generation. The expression of specific surface marker genes in isolated cells were identified by immunofluorescence and RT-PCR techniques, the results showed that CD29, CD73, CD90 and CD166 expressed, while CD45 didn' t express. After induction of adipogenesis, lipid droplets stained with oil red stain were seen, and adipogenic genes LPL and PPAR-γ both expressed. After induction of osteogenic differentiation, calcium nodules were stained with alizarin red, and osteogenic genes OPN and collagen type I gene COL-I expressed; after induction of chondrogenic differentiation, the chondrocytes were stained with Alisin blue, and they expressed chondrogenic key gene transcription factor gene SOX9 and ACAN. In this study, mesenchymal stem cells with good proliferation viability and adipogenic, osteogenic and chondrogenic abilities were successfully isolated from the umbilical cord of Holstein cattle at 3-month-old embryonic, which provided basis for the conservation of animal germplasm resources and regenerative medicine as well as potential cell resources. [ABSTRACT FROM AUTHOR]

Bone marrow mesenchymal stem cells (BMSCs) derived from the early development of the mesoderm and ectoderm, as seed cells in stem cells, with strong self-replication and renewal ability, and have repair and therapeutic effects for the damaged or defective liver, muscle and other tissues. In order to study BMSCs, this article conducted in vitro isolation and culture and biological identification of Japanese large ear white rabbit bone marrow mesenchymal stem cells. The bone marrow tissue of Japanese large ear white rabbit was isolated, purified and cultured in vitro by whole bone marrow adhesion method to identify the biological characteristics of Japanese large ear white rabbit BMSCs, observe cell morphology, detect growth kinetics, and identify osteogenesis, cartilage and adipogenic ability. The results showed that BMSCs with good growth status were successfully isolated from the bone marrow of Japanese large ear white rabbits by whole bone marrow adhesion, with long spindle shape and a typical "S" shape in the growth curve. The results of immunofluorescence and RT-PCR showed that BMSCs of Japanese large ear white rabbits expressed CD29, CD44, CD73, CD105 mesenchymal stem cell surface marker genes, but didn t express the original hematopoietic progenitor cell marker CD45. Specific staining and RT-PCR showed that the isolated cells were mesenchymal stem cells; in vitro induced BMSCs of Japanese large ear white rabbits to differentiate into bone, cartilage and fat cells. In this study, an in vitro isolation and culture system for BMSCs in Japanese large ear white rabbits was established, which laid a theoretical foundation for the use of bone marrow mesenchymal stem cells as tissue and engineering research and the conservation of animal genetic resources. animal genetic resources. [ABSTRACT FROM AUTHOR]

Lead (Pb) is a critical industrial and environmental contaminant that can cause pathophysiological changes in several cellular and organ systems and their processes, including cell proliferation, differentiation, apoptosis, and survival. The skin is readily exposed to and damaged by Pb, but the mechanisms through which Pb damages cells are not fully understood. We examined the apoptotic properties of Pb in mouse skin fibroblast (MSF) in vitro. Treatment of fibroblasts with 40, 80, and 160 μM Pb for 24 h revealed morphological alterations, DNA damage, enhanced caspase-3, -8, and -9 activities, and apoptotic cell population. Furthermore, apoptosis was dosage (0–160 μM) and time (12–48 h) dependent. Concentrations of intracellular calcium (Ca2+) and reactive oxygen species were increased, and the mitochondrial membrane potential was decreased in exposed cells. Cell cycle arrest was evident at the G0/G1 phase. The Bax, Fas, caspase-3 and -8, and p53 transcript levels were increased, whereas Bcl-2 gene expression was decreased. Based on our analysis, Pb triggers MSF apoptosis bydisrupting intracellular homeostasis. Our findings enrich the knowledge about the mechanistic function of Pb-induced cytotoxicity on human skin fibroblasts and could potentially guide future Pb health risk assessments. [ABSTRACT FROM AUTHOR]