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11 results on '"Guan, Shihui"'

4. Multifunctional Phase-Transition Nanoparticles for Effective Targeted Sonodynamic-Gene Therapy Against Thyroid Papillary Carcinoma

Author

Guan,Shihui, Teng,Dengke, Wang,Hui, Wang,Qimeihui, Zhen,Xi, Sui,Guoqing, Wang,Yang, Zhu,Lingyu, Lin,Yuanqiang, Jiao,Dan, Guo,Feng, Guan,Shihui, Teng,Dengke, Wang,Hui, Wang,Qimeihui, Zhen,Xi, Sui,Guoqing, Wang,Yang, Zhu,Lingyu, Lin,Yuanqiang, Jiao,Dan, and Guo,Feng

Abstract

Shihui Guan, Dengke Teng, Hui Wang,* Qimeihui Wang,* Xi Zhen, Guoqing Sui, Yang Wang, Lingyu Zhu, Yuanqiang Lin, Dan Jiao, Feng Guo Department of Ultrasound, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, 130033, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hui Wang; Qimeihui Wang, Email whui66@jlu.edu.cn; wangqmh@jlu.edu.cnIntroduction: In order to diagnose and treat papillary thyroid carcinoma (PTC) accurately, phase-transition nanoparticles, P@IP-miRNA (PFP@IR780/PLGA-bPEI-miRNA338-3p), was engineered. The nanoparticles (NPs) can target the tumor cells, realize the multimodal imaging, and provide sonodynamic-gene therapy for PTC.Methods: P@IP-miRNA NPs were synthesized through double emulsification method, and miRNA338-3p was attached to the surface of the NPs by electrostatic adsorption. The characterization of NPs was detected to screen out qualified nanoparticles. In vitro, laser confocal microscopy and flow cytometry were used to detect the targeting and subcellular localization of NPs. Western blot, qRT-PCR, and immunofluorescence were used to detect the ability to transfect miRNA. CCK8 kit, laser confocal microscopy and flow cytometry were used to detect the inhibition on TPC-1 cells. In vivo experiments were performed based on tumor-bearing nude mice. The efficacy of combined treatment by NPs was comprehensively evaluated, and the multimodal imaging ability of NPs in vivo and in vitro was detected.Results: P@IP-miRNA NPs were successfully synthesized which have spherical shape, uniform size, good dispersion and positive potential. The encapsulation rate of IR780 was (82.58± 3.92) %, the drug loading rate was (6.60± 0.32) %, and the adsorption capacity of miRNA338-3p was 41.78 μg/mg. NPs have excellent tumor targeting ability, miRNA transfection ability, ROS production ability and multimodal imaging ability in vivo and in vitro. The antitumor effect of combi

Published
2023

6. Microgel emulsion loaded with recombinant oil body as a new drug delivery carrier to promote wound repair

Author

Xinxin Lan, Lin Shuangzhu, Guan Shihui, Jing Li, Weidong Qiang, Linna Du, Tingting zhou, Yue Dong, Xiaokun Li, and Jing Yang

Abstract

Background: The skin is an important barrier to protect the body of external damage, there are tens of millions of patients with various skin defects. Due to the complex process of wound repair, it is easy to cause poor healing and other problems. Here, we prepared a new type of transdermal oil body microgel emulsion (OBEME) as a delivery carrier and preliminarily expounded its effect on wound healing. Method: The basic properties of the OBEME were characterized by scanning electron microscope, cryo-scanning electron microscopy, rheology and thermos- gravimetric analysis. Meanwhile, after 90 d of storage at room temperature, the effects of low-speed centrifugation and repeated freeze-thaw cycles on OBEME were investigated by particle size, potential and fluorescence microscopy tests. Through in vivo transdermal experiments and construction of a full-thickness wound model, the pharmacological effects of OBEME were clarified. Results: The OBEME is an interconnected network and presents to be smooth with no presence of pores, at the same time, after 90 d of storage at room temperature, the microstructure showed that the oil bodies remained intact. Unaffected by low-speed centrifugation and repeated freeze-thaw cycles. When OBEME treated the mouse skin for 90min, the EGF content in the mouse skin tissue reached the highest, the skin tissue gap was enlarged, and the skin permeability was enhanced. The wound coverage rate exceeded 98 % after treatment for 15 d with OBEME. After OBEME treatment, the inflammatory factor CD68 in the wound tissue was reduced, and the formation of new blood vessels, cytokeratin K5, K19 and collagen was promoted.Conclusions: The prepared OBEME has good stability, it can alter skin permeability, and promote wound healing by regulating inflammatory response, accelerating angiogenesis, promoting re-epithelialization and remodeling. The OBEME is a new carrier for percutaneous drug delivery, which lays a foundation for the development of new topical drugs.

Published
2022
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