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35 results on '"Guan, Jiuqiang"'

11. Ruminal pH pattern, fermentation characteristics and related bacteria in response to dietary live yeast (Saccharomyces cerevisiae) supplementation in beef cattle

Author

Xiaolin Luo, Ali Mujtaba Shah, Metha Wanapat, Xianwen Dong, Xiangfei Zhang, Guan Jiuqiang, Rui Hu, Quanhui Peng, Kun Kang, and Zhisheng Wang

Subjects
Physiology, ph, Total mixed ration, Beef cattle, Article, Ruminant Nutrition and Forage Utilization, Rumen, beef cattle, Genetics, Selenomonas ruminantium, Food science, chemistry.chemical_classification, General Veterinary, Yeast, rumen fermentation, chemistry, Gluconeogenesis, QL1-991, Propionate, Saccharomyces Cerevisiae, Animal Science and Zoology, Fermentation, rumen microorganism, serum, Zoology, Food Science
Abstract

Objective: In this study we aimed to evaluate the effect of dietary live yeast supplementation on ruminal pH pattern, fermentation characteristics and associated bacteria in beef cattle.Methods: This work comprised of in vitro and in vivo experiments. In vitro fermentation was conducted by incubating 0%, 0.05%, 0.075%, 0.1%, 0.125%, and 0.15% active dried yeast (Saccharomyces cerevisiae, ADY) with total mixed ration substrate to determine its dose effect. According to in vitro results, 0.1% ADY inclusion level was assigned in in vivo study for continuously monitoring ruminal fermentation characteristics and microbes. Six ruminally cannulated steers were randomly assigned to 2 treatments (Control and ADY supplementation) as two-period crossover design (30-day). Blood samples were harvested before-feeding and rumen fluid was sampled at 0, 3, 6, 9, and 12 h post-feeding on 30 d.Results: After 24 h in vitro fermentation, pH and gas production were increased at 0.1% ADY where ammonia nitrogen and microbial crude protein also displayed lowest and peak values, respectively. Acetate, butyrate and total volatile fatty acids concentrations heightened with increasing ADY doses and plateaued at high levels, while acetate to propionate ratio was decreased accordingly. In in vivo study, ruminal pH was increased with ADY supplementation that also elevated acetate and propionate. Conversely, ADY reduced lactate level by dampening Streptococcus bovis and inducing greater Selenomonas ruminantium and Megasphaera elsdenii populations involved in lactate utilization. The serum urea nitrogen decreased, whereas glucose, albumin and total protein concentrations were increased with ADY supplementation.Conclusion: The results demonstrated dietary ADY improved ruminal fermentation dosedependently. The ruminal lactate reduction through modification of lactate metabolic bacteria could be an important reason for rumen pH stabilization induced by ADY. ADY supplementation offered a complementary probiotics strategy in improving gluconeogenesis and nitrogen metabolism of beef cattle, potentially resulted from optimized rumen pH and fermentation.

Published
2022

21. Comparative transcriptome analysis of winter yaks in plateau and plain.

Author

Wang, Jiabo, Guan, Jiuqiang, Yixi, Kangzhu, Shu, Tao, Chai, Zhixin, Wang, Jikun, Wang, Hui, Wu, Zhijuan, Cai, Xin, Zhong, Jincheng, and Luo, Xiaolin

Subjects
*YAK, *BEEF quality, *PLAINS, *GENE expression, *COMPARATIVE studies
Abstract

The yak is an important source for the people living and ecological environment in the Qinghai–Tibet Plateau. In every winter, many domestic yaks will lose bodyweight or dead under cold and food scarcity. Moving the plateau yaks to farm in the plain is a useful approach to reduce their environmental stress and gain more production. In this study, we measured growth, slaughter and beef quality traits every month and sequenced mRNA expression levels of muscles of two groups yaks living in plateau and plain respectively. We found there is significant difference (p‐value <0.01) in the third (60 days), fourth (90 days), fifth (120 days) and sixth (150 days) weights between subpopulations in the plateau and plain. We identified 540 different expressed genes (DEGs) including 123 known genes and 417 unknown genes. Using the weighted correlation network analysis (WGCNA) to build a co‐express network, the modules were strong relative to weight traits. The findings highlighted the underlying way and a relative network to yield a new view about gene expression between the yaks living plateau and plain. [ABSTRACT FROM AUTHOR]

Published
2022
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23. Comparative transcriptomics of 5 high-altitude vertebrates and their low-altitude relatives

Author

Tang, Qianzi, primary, Gu, Yiren, additional, Zhou, Xuming, additional, Jin, Long, additional, Guan, Jiuqiang, additional, Liu, Rui, additional, Li, Jing, additional, Long, Kereng, additional, Tian, Shilin, additional, Che, Tiandong, additional, Hu, Silu, additional, Liang, Yan, additional, Yang, Xuemei, additional, Tao, Xuan, additional, Zhong, Zhijun, additional, Wang, Guosong, additional, Chen, Xiaohui, additional, Li, Diyan, additional, Ma, Jideng, additional, Wang, Xun, additional, Mai, Miaomiao, additional, Jiang, An’an, additional, Luo, Xiaolin, additional, Lv, Xuebin, additional, Gladyshev, Vadim N, additional, Li, Xuewei, additional, and Li, Mingzhou, additional

Published
2017
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24. Comparative analysis of the microRNA transcriptome between yak and cattle provides insight into high-altitude adaptation

Author

Guan, Jiuqiang, primary, Long, Keren, additional, Ma, Jideng, additional, Zhang, Jinwei, additional, He, Dafang, additional, Jin, Long, additional, Tang, Qianzi, additional, Jiang, Anan, additional, Wang, Xun, additional, Hu, Yaodong, additional, Tian, Shilin, additional, Jiang, Zhi, additional, Li, Mingzhou, additional, and Luo, Xiaolin, additional

Published
2017
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26. Effects of Starvation on Lipid Metabolism and Gluconeogenesis in Yak

Author

Tianwu An, Guan Jiuqiang, Zhisheng Wang, Xiaoqiang Yu, Xiaolin Luo, and Quanhui Peng

Subjects
0301 basic medicine, medicine.medical_specialty, lcsh:Animal biochemistry, Hormone-sensitive lipase, Ketone Bodies, Article, 03 medical and health sciences, chemistry.chemical_compound, Internal medicine, medicine, lcsh:QP501-801, lcsh:SF1-1100, Glycogen, 0402 animal and dairy science, Gluconeogenesis, 04 agricultural and veterinary sciences, 040201 dairy & animal science, 030104 developmental biology, Endocrinology, Acetoacetic acid, chemistry, Biochemistry, Starvation, Ketone bodies, Fat Catabolism, Animal Science and Zoology, Maiwa Yak, Pyruvic acid, Sample collection, lcsh:Animal culture, Starvation response, Food Science
Abstract

This research was conducted to investigate the physiological consequences of undernourished yak. Twelve Maiwa yak (110.3±5.85 kg) were randomly divided into two groups (baseline and starvation group). The yak of baseline group were slaughtered at day 0, while the other group of yak were kept in shed without feed but allowed free access to water, salt and free movement for 9 days. Blood samples of the starvation group were collected on day 0, 1, 2, 3, 5, 7, 9 and the starved yak were slaughtered after the final blood sample collection. The liver and muscle glycogen of the starvation group decreased (p

Published
2015

28. Breed, gender and anatomic location-specific expression patterns of the porcine insig-1 and insig-2 genes

Author

Liu Yingkai, Guan Jiuqiang, Li Xuewei, and Li Mingzhou

Subjects
Messenger RNA, medicine.medical_specialty, Cell, General Engineering, General Physics and Astronomy, Adipose tissue, General Medicine, Biology, General Biochemistry, Genetics and Molecular Biology, Breed, chemistry.chemical_compound, medicine.anatomical_structure, Endocrinology, chemistry, Internal medicine, Adipocyte, Lipogenesis, medicine, General Agricultural and Biological Sciences, Gene, Fatty acid synthesis
Abstract

The insulin-induced genes (insig-1 and insig-2) played important roles inadipocyte differentiation and lipogenesis. To determine the breed, gender and anatomic location-specific expression patterns of insig-1 and insig-2, we measured their mRNA abundances in different adipose tissues from male and female pigs for leaner and fatty breeds using a qRT-PCR approach. In accordance with the well-characterized role of insig-1 and insig-2 in promoting fatty acid synthesis, we found that, compared with the leaner breed, the fatty breed had the higher mRNA abundances of the two genes. The subcutaneous adipose tissues with the higher adipocyte cell diameters showed the higher mRNA abundance for the two genes compared with the mRNA abundance in the visceral adipose tissues. The clear positive correlation between adipocyte cell diameter and mRNA abundance of insig-1 and insig-2 also highlighted their critical roles in adipose deposition. These results suggested that insig-1 and insig-2 were related to porcine adipose deposition and are thus potential candidate genes for the pig fat mass trait. Key words: Insulin-induced gene 1 and 2, pig, adipose deposition, qRT-PCR.

Published
2012

31. An atlas of DNA methylomes in porcine adipose and muscle tissues

Author

Li, Mingzhou, primary, Wu, Honglong, additional, Luo, Zonggang, additional, Xia, Yudong, additional, Guan, Jiuqiang, additional, Wang, Tao, additional, Gu, Yiren, additional, Chen, Lei, additional, Zhang, Kai, additional, Ma, Jideng, additional, Liu, Yingkai, additional, Zhong, Zhijun, additional, Nie, Jing, additional, Zhou, Shuling, additional, Mu, Zhiping, additional, Wang, Xiaoyan, additional, Qu, Jingjing, additional, Jing, Long, additional, Wang, Huiyu, additional, Huang, Shujia, additional, Yi, Na, additional, Wang, Zhe, additional, Xi, Dongxing, additional, Wang, Juan, additional, Yin, Guangliang, additional, Wang, Li, additional, Li, Ning, additional, Jiang, Zhi, additional, Lang, Qiulei, additional, Xiao, Huasheng, additional, Jiang, Anan, additional, Zhu, Li, additional, Jiang, Yanzhi, additional, Tang, Guoqing, additional, Mai, Miaomiao, additional, Shuai, Surong, additional, Li, Kui, additional, Wang, Jinyong, additional, Zhang, Xiuqing, additional, Li, Yingrui, additional, Chen, Haosi, additional, Gao, Xiaolian, additional, Plastow, Graham S., additional, Beck, Stephen, additional, Yang, Huanming, additional, Wang, Jian, additional, Wang, Jun, additional, Li, Xuewei, additional, and Li, Ruiqiang, additional

Published
2012
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33. Repertoire of Porcine MicroRNAs in Adult Ovary and Testis by Deep Sequencing

Author

Li, Mingzhou, primary, Liu, Yingkai, additional, Wang, Tao, additional, Guan, Jiuqiang, additional, Luo, Zonggang, additional, Chen, Haosi, additional, Wang, Xin, additional, Chen, Lei, additional, Ma, Jideng, additional, Mu, Zhiping, additional, Jiang, An-an, additional, Zhu, Li, additional, Lang, Qiulei, additional, Zhou, Xiaochuan, additional, Wang, Jinyong, additional, Zeng, Wenxian, additional, Li, Ning, additional, Li, Kui, additional, Gao, Xiaolian, additional, and Li, Xuewei, additional

Published
2011
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34. MicroRNAome of Porcine Pre- and Postnatal Development

Author

Li, Mingzhou, primary, Xia, Youlin, additional, Gu, Yiren, additional, Zhang, Kai, additional, Lang, Qiulei, additional, Chen, Lei, additional, Guan, Jiuqiang, additional, Luo, Zonggang, additional, Chen, Haosi, additional, Li, Yang, additional, Li, Qinghai, additional, Li, Xiang, additional, Jiang, An-an, additional, Shuai, Surong, additional, Wang, Jinyong, additional, Zhu, Qi, additional, Zhou, Xiaochuan, additional, Gao, Xiaolian, additional, and Li, Xuewei, additional

Published
2010
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35. Whole-genome resequencing reveals genetic diversity, differentiation, and selection signatures of yak breeds/populations in southwestern China.

Author

Zhang S, Li J, Zhao Y, Tang Y, Li H, Song T, An T, Guan J, Li X, and Zhang M

Abstract

The Sichuan-Yunnan region is the main production area of yaks in southwestern China, with rich genetic resources of Yaks. Nevertheless, there have been limited study on the genetic characteristics of the entire yak populations in Tibet and southwestern China. In this study, we performed whole-genome resequencing to identify genetic variation information in a total of 198 individuals from six yak breeds (populations) in Sichuan (Muli yak, Jinchuan yak, Changtai yak, Maiwa yak), Yunnan (Zhongdian yak), and Tibet (Tibetan yak). The aim was to investigate the whole-genome genetic diversity, population genetic structure, and genome selection signatures. We observed that all six populations exhibit abundant genetic diversity. Except for Tibetan yaks, which showed low nucleotide diversity (0.00104), the remaining yak populations generally displayed high nucleotide diversity (0.00129-0.00153). Population genetic structure analysis revealed that, among the six yak populations, Muli yak exhibited greater differentiation from other yak populations and formed a distinct cluster independently. The Maiwa yak population displayed a complex genetic structure and exhibited gene exchange with Jinchuan and Changtai yaks. Positive selection signals were detected in candidate genes associated with growth ( GNB4 , HMGA2 , TRPS1 , and LTBP1 ), reproduction ( PI4KB , DYNC1I1 , and GRIP1 ), immunity ( CD200 and IL1RAP ), lactation ( SNX13 and CPM ), hypoxia adaptation ( NDUFB6 , PRKN , and MRPS9 ), hair ( KRT24 , KRT25 , and KRT26 ), meat quality ( SUCLG2 ), digestion and absorption ( CLDN1 ), and pigment deposition ( OCA2 ) using the integrated Pi and F ST methods. This study provides significant insights into understanding the whole-genome genetic characteristics of yak populations in Tibet and southwestern China., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zhang, Li, Zhao, Tang, Li, Song, An, Guan, Li and Zhang.)

Published
2024
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