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This document is a correction notice for an article titled "Targeted Extracellular Vesicles Delivered Verrucarin A to Treat Glioblastoma" published in the journal Biomedicines. The correction states that there was an error in Figure 6 of the original publication, and a corrected version of the figure is provided. The authors assure that the scientific conclusions of the article are unaffected by this error. The correction was approved by the Academic Editor, and the original publication has been updated accordingly. The article includes a graph showing the in vivo evaluation of the anti-GBM efficacy of mAb-EV-Ver-A, as well as representative images and H&E staining of brain tissue sections. The authors of the article are Kai Chen, Yingnan Si, Jia-Shiung Guan, Zhuoxin Zhou, Seulhee Kim, Taehyun Kim, Liang Shan, Christopher D. Willey, Lufang Zhou, and Xiaoguang Liu. [Extracted from the article]

Adeno-associated viruses (AAVs) have been well characterized and used to deliver therapeutic genes for diseases treatment in clinics and basic research. This study used the triple transient transfection of AAV-DJ/8 as a model expression system to develop and optimize the laboratory production of AAV for research and pre-clinical applications. Specifically, various production parameters, including host cell, transfection reagent, cell density, ratio of plasmid DNA and cells, gene size, and production mode, were tested to determine the optimal process. Our results showed that the adherent production using HEK 293AAV with calcium transfection generated the highest volumetric productivity of 7.86x10 9 gc/mL. The optimal suspensive production using HEK 293F had best AAV productivity of 5.78x10 9 gc/mL in serum-free medium under transfection conditions of transfection density of 0.4x10 6 cells/mL, plasmid DNA:cells ratio of 1.6 μg:10 6 cells and synthesized cationic liposomes as transfection reagent. The similar AAV productivity was confirmed at scales of 30 mL - 450 mL in shaker and/or spinner flasks. The in vitro transfection and in vivo infection efficiency of the harvested AAV-DJ/8 carrying luciferase reporter gene was confirmed using cell line and xenograft mouse model, respectively. The minimal or low purification recovery rate of AAV-DJ/8 in ion-exchange chromatography column and affinity column was observed in this study. In summary, we developed and optimized a scalable suspensive production of AAV to support the large-scale preclinical animal studies in research laboratories., Competing Interests: Conflicts of Interest The authors declare no conflict of interest.