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495 results on '"Gu Jiayu"'

1. Analysis on Local Adhesion Differences of Hot Dip Galvanized Coating on Low-Alloy High-Strength Angle Steel

Author

GU Jiayu, LIANG Guowei, LAI Delin, WAN Xianlan, CHE Chunshan

Subjects
high-strength steel, galvanizing, bonding, transmission tower, Materials of engineering and construction. Mechanics of materials, TA401-492, Technology
Abstract

The reasons for the local adhesion differences of the hot-dip galvanized coating on high-strength angle steel were analyzed by means of scanning electron microscopy (SEM) and optical microscopy (OM)， and the microstructures of the coating and steel base in the good bonding area and poor bonding area of galvanized angle steel were compared. Results showed that: (1) The adhesion difference of the coating on steel substrate were closely related to the microstructure of the caoting. The coating area with good bonding strength was characterized with small column-like or lumpy ζ phase and discontinuous δ phase. When the coating suffering hammering， the hammered area was generally in the form of pits， and the alloy layer was extruded and deformed without cracks. The coating area with poor bonding strength consisted of a coarse columnar or bulky ζ phase and a continuous and thick δ layer， which would be stripped from the substrate interface when the coating suffering hammering. (2) Moreover， the surface microstructure of steel substrate had influence on the galvanized coating. During galvanizing， equilibrium structure was in favor of the diffusion of Fe， facilitating the growth of ζ and limiting the growth of δ， which was beneficial to the bonding between coating and substrate. However， the appearance of non-equilibrium structure could inhibit the diffusion of Fe， lower the growth of ζ and benefit to the growth of δ because of the solution strengthening，lattice distortion and internal residual stress，which was harmful for the bonding strength of hot-dip galvanized coating.

Published
2022
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32. Genetic Analysis and Fine Mapping of QTL for the Erect Leaf in Mutant mths29 Induced through Fast Neutron in Wheat

Author

Yang, Zhixin, primary, Gu, Jiayu, additional, Zhao, Minghui, additional, Fan, Xiaofeng, additional, Guo, Huijun, additional, Xie, Yongdun, additional, Zhang, Jinfeng, additional, Xiong, Hongchun, additional, Zhao, Linshu, additional, Zhao, Shirong, additional, Ding, Yuping, additional, Kong, Fuquan, additional, Sui, Li, additional, Xu, Le, additional, and Liu, Luxiang, additional

Published
2024
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38. Establishment of human corneal epithelial organoids for ex vivo modelling dry eye disease.

Author

Wan, Xichen, Gu, Jiayu, Zhou, Xujiao, Le, Qihua, Wang, Jingyuan, Xin, ChangChang, Chen, Zhi, He, Yao, and Hong, Jiaxu

Abstract

Dry eye disease (DED) is a growing public health concern affecting millions of people worldwide and causing ocular discomfort and visual disturbance. Developing its therapeutic drugs based on animal models suffer from interspecies differences and poor prediction of human trials. Here, we established long‐term 3D human corneal epithelial organoids, which recapitulated the cell lineages and gene expression signature of the human corneal epithelium. Organoids can be regulated to differentiate ex vivo, but the addition of FGF10 inhibits this process. In the hyperosmolar‐induced DED organoid model, the release of inflammatory factors increased, resulting in damage to the stemness of stem cells and a decrease in functional mucin 1 protein. Furthermore, we found that the organoids could mimic clinical drug treatment responses, suggesting that corneal epithelial organoids are promising candidates for establishing a drug testing platform ex vivo. In summary, we established a functional, long‐term 3D human epithelial organoid that may serve as an ex vivo model for studying the functional regulation and disease modelling. [ABSTRACT FROM AUTHOR]

Published
2024
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39. Rapid detection of guttae area using aniline blue staining in Fuchs endothelial corneal dystrophy mouse model.

Author

Zhang, Xueling, Qiu, Jini, Feng, Yalan, Zheng, Jijia, Xiang, Jun, Gu, Jiayu, Shan, Kun, and Shi, Qian

Subjects
CORNEAL dystrophies, STAINS & staining (Microscopy), GEOGRAPHIC boundaries, VISUAL acuity, LABORATORY mice
Abstract

Fuchs endothelial corneal dystrophy (FECD) is a leading cause of corneal endothelial degeneration resulting in impaired visual acuity. Excessive deposition of extracellular matrix (guttae) on Descemet's membrane (DM) is the hallmark of FECD. We sought to detect the guttae area rapidly using aniline blue (AB) staining in FECD mouse model. FECD mouse model was established via ultraviolet A (UVA) exposure. Masson's trichrome staining was utilized to stain the corneal sections. AB staining was utilized to stain both whole cornea tissues and stripped Descemet's membrane‐endothelium complex (DMEC) flat mounts, while immunofluorescence staining of collagen I was employed to stain guttae areas. In Masson's trichrome staining, corneal collagen fibrils were stained blue with AB. The DMEC flat mounts were stained into relative dark blue areas and relative light blue areas using 2% AB staining. The areas of dark blue could almost overlap with collagen I‐positive areas, and have an acellular centre and a moderately distinct boundary line with the surrounding corneal endothelial cells. In conclusion, AB staining is a rapid and effective method for the evaluation of the guttae areas in the FECD mouse model. [ABSTRACT FROM AUTHOR]

Published
2024
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40. Multi‐dataset identification of innovative feature genes and molecular mechanisms in keratoconus.

Author

Lyu, Ning, Dai, Yiqin, Wu, Jiawen, Fan, Yidan, Lyu, Zhaoyuan, Gu, Jiayu, Cheng, Jingyi, and Xu, Jianjiang

Subjects
GENE expression, DOWNLOADING, IMMUNOLOGIC memory, PLASMA cells, GENE expression profiling
Abstract

This study aimed to identify feature genes and explore the molecular mechanisms of keratoconus (KC). We downloaded data files from NCBI GEO public database. The Limma package was used for differential expression analysis of gene profiles. Lasso regression was used to identify the feature genes. The CIBERSORT algorithm was used to infer the proportion of immune‐infiltrating cells and analyse the correlation between gene expression levels and immune cells. Related transcription factors and miRNAs of key genes were predicted using the Cistrome DB and Mircode databases. Analysis of expression differences in disease genes was based on the GeneCards database. The CMap was used to analyse targeted therapeutic drugs. IHC was performed to verify the expression levels of ATOH7 and MYRF in corneas. Exactly 593 upregulated and 473 downregulated genes were identified. Lasso regression analysis identified ATOH7, DBNDD1, RNF217‐AS1, ARL11, MYRF and SNORA74B as feature genes for KC. All key genes were correlated with immune infiltration and the levels of activated memory CD4+ T cells and plasma cells were significantly increased. miRNA, IRF and STAT families were correlated to feature genes. The expression levels of key genes were significantly correlated to KC‐related genes. Entinostat, ochratoxin‐a, diphencyprone and GSK‐3‐inhibitor‐II were predicted as potential KC medications. The expression of MYRF was significantly higher in the KC samples, contrary to the expression of ATOH7. KC is related to both immune infiltration and genetic factors. MYRF and ATOH7 were newly identified and verified feature genes of KC. [ABSTRACT FROM AUTHOR]

Published
2024
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48. A hypoplastic model for unsaturated sand accounting for drying and wetting cycles

Author

Ni, Junjun, Gu, Jiayu, Zhao, Xudong, Ni, Junjun, Gu, Jiayu, and Zhao, Xudong

Abstract

Existing constitutive models for unsaturated sand cannot capture the irreversible volumetric strain in the drying and wetting (DW) cycles. This study develops a novel hypoplastic model that aims to capture the behaviour of unsaturated soils, with a specific emphasis on the DW cycles experienced by unsaturated sand. The formulation integrates a hypoplastic model proposed by von Wolffersdorff for saturated sand with Bishop's effective stress. The simulation of soil water characteristic curve hysteresis is achieved through the implementation of the scanning rule for the main drying/wetting curve and scanning curve. The incorporation of a new tensorial term, Hs, into the hypoplastic equations, captures the irreversible volumetric strain in the DW cycle. A novel pyknotropy factor, referred to as fus, is proposed to replicate the rate at which irreversible volumetric strain accumulates during the DW cycle. Furthermore, the influence of suction effects on the critical state line is taken into account to represent the shear behaviour of unsaturated sand. The evaluation of the model performance is conducted through existing element tests on unsaturated sand. The comparison illustrates that the proposed model has the capability to capture certain essential characteristics, such as irreversible volumetric strain in the DW cycles and critical state behaviour of unsaturated sand. Due to its simplicity, the proposed model lends itself to straightforward implementation and potential utilization in future engineering applications, particularly in the analysis of slope stability in the presence of rainfall-induced conditions. © 2023

Published
2024

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