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26 results on '"Grybchuk, Danyil"'

2. Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris

Author

Macedo, Diego H., Grybchuk, Danyil, Režnarová, Jana, Votýpka, Jan, Klocek, Donnamae, Yurchenko, Tatiana, Ševčík, Jan, Magri, Alice, Dolinská, Michaela Urda, Záhonová, Kristína, Lukeš, Julius, Servienė, Elena, Jászayová, Alexandra, Serva, Saulius, Malysheva, Marina N., Frolov, Alexander O., Yurchenko, Vyacheslav, and Kostygov, Alexei Yu.

Published
2023
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4. Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics

Author

Salamaga, Bartłomiej, Kong, Lingyuan, Pasquina-Lemonche, Laia, Lafage, Lucia, von und zur Muhlen, Milena, Gibson, Josie F., Grybchuk, Danyil, Tooke, Amy K., Panchal, Viralkumar, Culp, Elizabeth J., Tatham, Elizabeth, O’Kane, Mary E., Catley, Thomas E., Renshaw, Stephen A., Wright, Gerard D., Plevka, Pavel, Bullough, Per A., Han, Aidong, Hobbs, Jamie K., and Foster, Simon J.

Published
2021

7. Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi

Author

Homola, Miroslav, primary, Büttner, Carina R., additional, Füzik, Tibor, additional, Křepelka, Pavel, additional, Holbová, Radka, additional, Nováček, Jiří, additional, Chaillet, Marten L., additional, Žák, Jakub, additional, Grybchuk, Danyil, additional, Förster, Friedrich, additional, Wilson, William H., additional, Schroeder, Declan C., additional, and Plevka, Pavel, additional

Published
2024
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8. Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi

Author

Homola, Miroslav, Büttner, Carina R., Füzik, Tibor, Křepelka, Pavel, Holbová, Radka, Nováček, Jiří, Chaillet, Marten L., Žák, Jakub, Grybchuk, Danyil, Förster, Friedrich, Wilson, William H., Schroeder, Declan C., Plevka, Pavel, Homola, Miroslav, Büttner, Carina R., Füzik, Tibor, Křepelka, Pavel, Holbová, Radka, Nováček, Jiří, Chaillet, Marten L., Žák, Jakub, Grybchuk, Danyil, Förster, Friedrich, Wilson, William H., Schroeder, Declan C., and Plevka, Pavel

Abstract

The globally distributed marine alga Emiliania huxleyi has cooling effect on the Earth’s climate. The population density of E. huxleyi is restricted by Nucleocytoviricota viruses, including E. huxleyi virus 201 (EhV-201). Despite the impact of E. huxleyi viruses on the climate, there is limited information about their structure and replication. Here, we show that the dsDNA genome inside the EhV-201 virion is protected by an inner membrane, capsid, and outer membrane. EhV-201 virions infect E. huxleyi by using fivefold vertices to bind to and fuse the virus’ inner membrane with the cell plasma membrane. Progeny virions assemble in the cytoplasm at the surface of endoplasmic reticulum–derived membrane segments. Genome packaging initiates synchronously with the capsid assembly and completes through an aperture in the forming capsid. The genome-filled capsids acquire an outer membrane by budding into intracellular vesicles. EhV-201 infection induces a loss of surface protective layers from E. huxleyi cells, which enables the continuous release of virions by exocytosis.

Published
2024

9. Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi

Author

Structural Biochemistry, Sub Structural Biochemistry, Homola, Miroslav, Büttner, Carina R., Füzik, Tibor, Křepelka, Pavel, Holbová, Radka, Nováček, Jiří, Chaillet, Marten L., Žák, Jakub, Grybchuk, Danyil, Förster, Friedrich, Wilson, William H., Schroeder, Declan C., Plevka, Pavel, Structural Biochemistry, Sub Structural Biochemistry, Homola, Miroslav, Büttner, Carina R., Füzik, Tibor, Křepelka, Pavel, Holbová, Radka, Nováček, Jiří, Chaillet, Marten L., Žák, Jakub, Grybchuk, Danyil, Förster, Friedrich, Wilson, William H., Schroeder, Declan C., and Plevka, Pavel

Published
2024

10. Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

Author

Grybchuk, Danyil, Akopyants, Natalia S., Kostygov, Alexei Y., Konovalovas, Aleksandras, Lye, Lon-Fye, Dobson, Deborah E., Zangger, Haroun, Fasel, Nicolas, Butenko, Anzhelika, Frolov, Alexander O., Votýpka, Jan, d’Avila-Levy, Claudia M., Kulich, Pavel, Moravcová, Jana, Plevka, Pavel, Rogozin, Igor B., Serva, Saulius, Lukeš, Julius, Beverley, Stephen M., and Yurchenko, Vyacheslav

Published
2018

11. Diversity of RNA viruses in the cosmopolitan monoxenous trypanosomatid Leptomonas pyrrhocoris

Author

Fagundes Macedo, Diego, Grybchuk, Danyil, Reznarova, Jana, Votypka, Jan, Klocek, Donnamae, Yurchenko, Tatiana, Sevcik, Jan, Magri, Alice, Dolinska, Michaela Urda, Zahonova, Kristina, Lukes, Julius, Serviene, Elena, Jaszayova, Alexandra, Serva, Saulius, Malysheva, Marina N., Frolov, Alexander O., Yurchenko, Vyacheslav, Kostygov, Alexei Yu., Fagundes Macedo, Diego, Grybchuk, Danyil, Reznarova, Jana, Votypka, Jan, Klocek, Donnamae, Yurchenko, Tatiana, Sevcik, Jan, Magri, Alice, Dolinska, Michaela Urda, Zahonova, Kristina, Lukes, Julius, Serviene, Elena, Jaszayova, Alexandra, Serva, Saulius, Malysheva, Marina N., Frolov, Alexander O., Yurchenko, Vyacheslav, and Kostygov, Alexei Yu.

Abstract

Background Trypanosomatids are parasitic flagellates well known because of some representatives infecting humans, domestic animals, and cultural plants. Many trypanosomatid species bear RNA viruses, which, in the case of human pathogens Leishmania spp., influence the course of the disease. One of the close relatives of leishmaniae, Leptomonas pyrrhocoris, has been previously shown to harbor viruses of the groups not documented in other trypanosomatids. At the same time, this species has a worldwide distribution and high prevalence in the natural populations of its cosmopolitan firebug host. It therefore represents an attractive model to study the diversity of RNA viruses.Results We surveyed 106 axenic cultures of L. pyrrhocoris and found that 64 (60%) of these displayed 2-12 double-stranded RNA fragments. The analysis of next-generation sequencing data revealed four viral groups with seven species, of which up to five were simultaneously detected in a single trypanosomatid isolate. Only two of these species, a tombus-like virus and an Ostravirus, were earlier documented in L. pyrrhocoris. In addition, there were four new species of Leishbuviridae, the family encompassing trypanosomatid-specific viruses, and a new species of Qinviridae, the family previously known only from metatranscriptomes of invertebrates. Currently, this is the only qinvirus with an unambiguously determined host. Our phylogenetic inferences suggest reassortment in the tombus-like virus owing to the interaction of different trypanosomatid strains. Two of the new Leishbuviridae members branch early on the phylogenetic tree of this family and display intermediate stages of genomic segment reduction between insect Phenuiviridae and crown Leishbuviridae.Conclusions The unprecedented wide range of viruses in one protist species and the simultaneous presence of up to five viral species in a single Leptomonas pyrrhocoris isolate indicate the uniqueness of this flagellate. This is likely determined by th

Published
2023
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12. First report of putative Leishmania RNA virus 2 (LRV2) in Leishmania infantum strains from canine and human visceral leishmaniasis cases in the southeast of Brazil

Author

Rêgo, Felipe Dutra, primary, da Silva, Eduardo Sérgio, additional, Lopes, Valeriana Valadares, additional, Teixeira-Neto, Rafael Gonçalves, additional, Belo, Vinícius Silva, additional, Fonseca Júnior, Antônio Augusto, additional, Pereira, Diego Andrade, additional, Pena, Heber Paulino, additional, Laurenti, Márcia Dalastra, additional, Araújo, Gabriela V, additional, da Matta, Vânia Lúcia Ribeiro, additional, Chouman, Islam Hussein, additional, Burrin, Thainá Bergantin, additional, Sandoval, Carmen M, additional, Barrouin-Melo, Stella Maria, additional, de Pinho, Flaviane Alves, additional, de Andrade, Hélida Monteiro, additional, Nunes, Ramon Vieira, additional, Gontijo, Célia Maria Ferreira, additional, Soccol, Vanete Thomaz, additional, Klocek, Donnamae, additional, Grybchuk, Danyil, additional, Macedo, Diego Henrique, additional, do Monte-Neto, Rubens Lima, additional, Yurchenko, Vyacheslav, additional, and Soares, Rodrigo Pedro, additional

Published
2023
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14. Capsid Structure of Leishmania RNA Virus 1

Author

Procházková, Michaela, primary, Füzik, Tibor, additional, Grybchuk, Danyil, additional, Falginella, Francesco Luca, additional, Podešvová, Lucie, additional, Yurchenko, Vyacheslav, additional, Vácha, Robert, additional, and Plevka, Pavel, additional

Published
2021
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15. The First Non-LRV RNA Virus in Leishmania

Author

Grybchuk, Danyil, primary, Macedo, Diego H., additional, Kleschenko, Yulia, additional, Kraeva, Natalya, additional, Lukashev, Alexander N., additional, Bates, Paul A., additional, Kulich, Pavel, additional, Leštinová, Tereza, additional, Volf, Petr, additional, Kostygov, Alexei Y., additional, and Yurchenko, Vyacheslav, additional

Published
2020
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19. Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

Author

Grybchuk, Danyil, primary, Akopyants, Natalia S., additional, Kostygov, Alexei Y., additional, Konovalovas, Aleksandras, additional, Lye, Lon-Fye, additional, Dobson, Deborah E., additional, Zangger, Haroun, additional, Fasel, Nicolas, additional, Butenko, Anzhelika, additional, Frolov, Alexander O., additional, Votýpka, Jan, additional, d’Avila-Levy, Claudia M., additional, Kulich, Pavel, additional, Moravcová, Jana, additional, Plevka, Pavel, additional, Rogozin, Igor B., additional, Serva, Saulius, additional, Lukeš, Julius, additional, Beverley, Stephen M., additional, and Yurchenko, Vyacheslav, additional

Published
2017
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20. Molecular mechanisms of thermal resistance of the insect trypanosomatid Crithidia thermophila

Author

UCL - SSS/DDUV - Institut de Duve, Ishemgulova, Aygul, Butenko, Anzhelika, Kortišová, Lucie, Boucinha, Carolina, Grybchuk-Ieremenko, Anastasiia, Morelli, Karina A., Tesařová, Martina, Kraeva, Natalya, Grybchuk, Danyil, Pánek, Tomáš, Flegontov, Pavel, Lukeš, Julius, Votýpka, Jan, Pavan, Márcio Galvão, Opperdoes, Frederik, Spodareva, Viktoria, d'Avila-Levy, Claudia M., Kostygov, Alexei Yu., Yurchenko, Vyacheslav, Yue, Bi-Song, UCL - SSS/DDUV - Institut de Duve, Ishemgulova, Aygul, Butenko, Anzhelika, Kortišová, Lucie, Boucinha, Carolina, Grybchuk-Ieremenko, Anastasiia, Morelli, Karina A., Tesařová, Martina, Kraeva, Natalya, Grybchuk, Danyil, Pánek, Tomáš, Flegontov, Pavel, Lukeš, Julius, Votýpka, Jan, Pavan, Márcio Galvão, Opperdoes, Frederik, Spodareva, Viktoria, d'Avila-Levy, Claudia M., Kostygov, Alexei Yu., Yurchenko, Vyacheslav, and Yue, Bi-Song

Abstract

In the present work, we investigated molecular mechanisms governing thermal resistance of a monoxenous trypanosomatid Crithidia luciliae thermophila, which we reclassified as a separate species C. thermophila. We analyzed morphology, growth kinetics, and transcriptomic profiles of flagellates cultivated at low (23°C) and elevated (34°C) temperature. When maintained at high temperature, they grew significantly faster, became shorter, with genes involved in sugar metabolism and mitochondrial stress protection significantly upregulated. Comparison with another thermoresistant monoxenous trypanosomatid, Leptomonas seymouri, revealed dramatic differences in transcription profiles of the two species with only few genes showing the same expression pattern. This disparity illustrates differences in the biology of these two parasites and distinct mechanisms of their thermotolerance, a prerequisite for living in warm-blooded vertebrates.

Published
2017

21. Molecular mechanisms of thermal resistance of the insect trypanosomatid Crithidia thermophila

Author

Ishemgulova, Aygul, primary, Butenko, Anzhelika, additional, Kortišová, Lucie, additional, Boucinha, Carolina, additional, Grybchuk-Ieremenko, Anastasiia, additional, Morelli, Karina A., additional, Tesařová, Martina, additional, Kraeva, Natalya, additional, Grybchuk, Danyil, additional, Pánek, Tomáš, additional, Flegontov, Pavel, additional, Lukeš, Julius, additional, Votýpka, Jan, additional, Pavan, Márcio Galvão, additional, Opperdoes, Fred R., additional, Spodareva, Viktoria, additional, d'Avila-Levy, Claudia M., additional, Kostygov, Alexei Yu., additional, and Yurchenko, Vyacheslav, additional

Published
2017
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22. Leptomonas seymouri: Adaptations to the Dixenous Life Cycle Analyzed by Genome Sequencing, Transcriptome Profiling and Co-infection with Leishmania donovani

Author

Kraeva, Natalya, primary, Butenko, Anzhelika, additional, Hlaváčová, Jana, additional, Kostygov, Alexei, additional, Myškova, Jitka, additional, Grybchuk, Danyil, additional, Leštinová, Tereza, additional, Votýpka, Jan, additional, Volf, Petr, additional, Opperdoes, Fred, additional, Flegontov, Pavel, additional, Lukeš, Julius, additional, and Yurchenko, Vyacheslav, additional

Published
2015
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24. Molecular Characterization of Leishmania RNA virus 2 in Leishmaniamajor from Uzbekistan.

Author

Kleschenko, Yuliya, Grybchuk, Danyil, Matveeva, Nadezhda S., Macedo, Diego H., Ponirovsky, Evgeny N., Lukashev, Alexander N., and Yurchenko, Vyacheslav

Subjects
*RNA viruses, *LEISHMANIA major, *CUTANEOUS leishmaniasis, *LEISHMANIA, *SEQUENCE analysis, *LEISHMANIA mexicana, *SPECIES
Abstract

Here we report sequence and phylogenetic analysis of two new isolates of Leishmania RNA virus 2 (LRV2) found in Leishmania major isolated from human patients with cutaneous leishmaniasis in south Uzbekistan. These new virus-infected flagellates were isolated in the same region of Uzbekistan and the viral sequences differed by only nineteen SNPs, all except one being silent mutations. Therefore, we concluded that they belong to a single LRV2 species. New viruses are closely related to the LRV2-Lmj-ASKH documented in Turkmenistan in 1995, which is congruent with their shared host (L. major) and common geographical origin. [ABSTRACT FROM AUTHOR]

Published
2019
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25. RNA Viruses in Blechomonas(Trypanosomatidae) and Evolution of Leishmaniavirus

Author

Grybchuk, Danyil, Kostygov, Alexei Y., Macedo, Diego H., Votýpka, Jan, Lukeš, Julius, and Yurchenko, Vyacheslav

Abstract

Flagellates belonging to the genus Leishmaniaare important human parasites. Some strains of different Leishmaniaspecies harbor viruses (leishmaniaviruses), which facilitate metastatic spread of the parasites, thus aggravating the disease. Up until now, these viruses were known to be hosted only by Leishmania. Here, we analyzed viral distribution in Blechomonas, a related group of flagellates parasitizing fleas, and revealed that they also bear leishmaniaviruses. Our findings shed light on the entangled evolution of these viruses. In addition, we documented that Blechomonascan be also infected by leishbunyaviruses and narnaviruses, viral groups known from other insects’ flagellates.

Published
2018
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26. Identification of diverse RNA viruses in Obscuromonas flagellates (Euglenozoa: Trypanosomatidae: Blastocrithidiinae).

Author

Grybchuk D, Galan A, Klocek D, Macedo DH, Wolf YI, Votýpka J, Butenko A, Lukeš J, Neri U, Záhonová K, Kostygov AY, Koonin EV, and Yurchenko V

Abstract

Trypanosomatids (Euglenozoa) are a diverse group of unicellular flagellates predominately infecting insects (monoxenous species) or circulating between insects and vertebrates or plants (dixenous species). Monoxenous trypanosomatids harbor a wide range of RNA viruses belonging to the families Narnaviridae, Totiviridae , Qinviridae , Leishbuviridae, and a putative group of tombus-like viruses. Here, we focus on the subfamily Blastocrithidiinae, a previously unexplored divergent group of monoxenous trypanosomatids comprising two related genera: Obscuromonas and Blastocrithidia . Members of the genus Blastocrithidia employ a unique genetic code, in which all three stop codons are repurposed to encode amino acids, with TAA also used to terminate translation. Obscuromonas isolates studied here bear viruses of three families: Narnaviridae, Qinviridae , and Mitoviridae . The latter viral group is documented in trypanosomatid flagellates for the first time. While other known mitoviruses replicate in the mitochondria, those of trypanosomatids appear to reside in the cytoplasm. Although no RNA viruses were detected in Blastocrithidia spp., we identified an endogenous viral element in the genome of B. triatomae indicating its past encounter(s) with tombus-like viruses., Competing Interests: None declared., (© The Author(s) 2024. Published by Oxford University Press.)

Published
2024
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