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4 results on '"Gruber, C.E.M."'

2. Geographical Variability Affects CCHFV Detection by RT-PCR: A Tool for In-Silico Evaluation of Molecular Assays

Author

Gruber, C.E.M. (Cesare E M), Bartolini, B. (Barbara), Castilletti, C. (Concetta), Mirazimi, A. (Ali), Hewson, R. (Roger), Christova, I., Avšič, T. (Tatjana), Grunow, R. (Roland), Papa, A. (Anna), Sanchez-Seco, M.P. (Maria Paz), Kopmans, M. (Marion), Ippolito, G. (Giuseppe), Capobianchi, M.R. (Maria Rosaria), Reusken, C.B.E.M. (Chantal), Caro, A.D. (Antonino Di), Gruber, C.E.M. (Cesare E M), Bartolini, B. (Barbara), Castilletti, C. (Concetta), Mirazimi, A. (Ali), Hewson, R. (Roger), Christova, I., Avšič, T. (Tatjana), Grunow, R. (Roland), Papa, A. (Anna), Sanchez-Seco, M.P. (Maria Paz), Kopmans, M. (Marion), Ippolito, G. (Giuseppe), Capobianchi, M.R. (Maria Rosaria), Reusken, C.B.E.M. (Chantal), and Caro, A.D. (Antonino Di)

Abstract

The Crimean-Congo hemorrhagic fever virus (CCHFV) is considered to be a major emerging infectious threat, according to the WHO R&D blueprint. A wide range of CCHFV molecular assays have been developed, employing varied primer/probe combinations. The high genetic variability of CCHFV often hampers the efficacy of available molecular tests and can affect their diagnostic potential. Recently, increasing numbers of complete CCHFV genomic sequences have become available, allowing a better appreciation of the genomic evolution of this virus. We summarized the current knowledge on molecular methods and developed a new bioinformatics tool to evaluate the existing assays for CCHFV detection, with a special focus on strains ci

Published
2019
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3. Laboratory management of Crimean-Congo haemorrhagic fever virus infections: perspectives from two European networks

Author

Bartolini, B., Gruber, C.E.M., Koopmans D.V.M., M.P.G. (Marion), Avsic, T., Bino, S., Christova, I., Grunow, R., Hewson, R., Korukluoglu, G., Lemos, C.M., Mirazimi, A., Papa, A, Sanchez-Seco, MP, Sauer, A.V. (Aisha), Zeller, H., Nisii, C., Capobianchi, M.R., Ippolito, G., Reusken, C.B.E.M. (Chantal), Di Caro, A, Bartolini, B., Gruber, C.E.M., Koopmans D.V.M., M.P.G. (Marion), Avsic, T., Bino, S., Christova, I., Grunow, R., Hewson, R., Korukluoglu, G., Lemos, C.M., Mirazimi, A., Papa, A, Sanchez-Seco, MP, Sauer, A.V. (Aisha), Zeller, H., Nisii, C., Capobianchi, M.R., Ippolito, G., Reusken, C.B.E.M. (Chantal), and Di Caro, A

Abstract

Background: Crimean-Congo haemorrhagic fever virus (CCHFV) is considered an emerging infectious disease threat in the European Union. Since 2000, the incidence and geographic range of confirmed CCHF cases have markedly increased, following changes in the distribution of its main vector, Hyalomma ticks. Aims: To review scientific literature and collect experts’ opinion to analyse relevant aspects of the laboratory management of human CCHF cases and any exposed contacts, as well as identify areas for advancement of international collaborative preparedness and laboratory response plans. Methods: We conducted a literature review on CCHF molecular diagnostics through an online search. Further, we obtained expert opinions on the key laboratory aspects of CCHF diagnosis. Consulted experts were members of two European projects, EMERGE (Efficient response to highly dangerous and emerging pathogens at EU level) and EVDLabNet (Emerging Viral Diseases-Expert Laborator

Published
2019
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4. Transcriptomic investigation of meat tenderness in two Italian cattle breeds

Author

Cesare Ernesto Maria Gruber, Fabrizio Ferrè, Susana Bueno, Bianca Moioli, S. Failla, Alessio Valentini, Giovanni Chillemi, Silvia Bongiorni, Bongiorni, S, Gruber, C.E.M., Bueno, S., Chillemi, G., Ferrè, F., Failla, S., Moioli, B., and Valentini, A.

Subjects
0301 basic medicine, Gene isoform, Meat, Maremmana, Muscle Proteins, Single-nucleotide polymorphism, AMP-Activated Protein Kinases, Breeding, Biology, Chianina, Polymorphism, Single Nucleotide, meat quality, 03 medical and health sciences, Meat tenderness, Genetic, Gene expression, Genetics, Animals, Allele, Muscle, Skeletal, Promoter Regions, Genetic, Gene, Alleles, Sequence Analysis, RNA, High-Throughput Nucleotide Sequencing, General Medicine, PRKAG3 gene, biology.organism_classification, Alternative Splicing, 030104 developmental biology, MRNA Sequencing, Italy, skeletal muscle transcriptome, Cattle, Animal Science and Zoology, Transcriptome
Abstract

Our objectives for this study were to understand the biological basis of meat tenderness and to provide an overview of the gene expression profiles related to meat quality as a tool for selection. Through deep mRNA sequencing, we analyzed gene expression in muscle tissues of two Italian cattle breeds: Maremmana and Chianina. We uncovered several differentially expressed genes that encode for proteins belonging to a family of tripartite motif proteins, which are involved in growth, cell differentiation and apoptosis, such as TRIM45, or play an essential role in regulating skeletal muscle differentiation and the regeneration of adult skeletal muscle, such as TRIM32. Other differentially expressed genes (SCN2B, SLC9A7 and KCNK3) emphasize the involvement of potassium-sodium pumps in tender meat. By mapping splice junctions in RNA-Seq reads, we found significant differences in gene isoform expression levels. The PRKAG3 gene, which is involved in the regulation of energy metabolism, showed four isoforms that were differentially expressed. This distinct pattern of PRKAG3 gene expression could indicate impaired glycogen storage in skeletal muscle, and consequently, this gene very likely has a role in the tenderization process. Furthermore, with this deep RNA-sequencing, we captured a high number of expressed SNPs, for example, we found 1462 homozygous SNPs showing the alternative allele with a 100% frequency when comparing tender and tough meat. SNPs were then classified into categories by their position and also by their effect on gene coding (174 non-synonymous polymorphisms) based on the available UMD_3.1 annotations.

Published
2016

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