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The aim of this study was to assess whether modified bacterial cellulose can be used for an artificial auricle skeleton. Introduction: The auricle is a part of the external ear. It consists of skin, cartilage, muscles and adipose tissue. The cartilage gives shape to the structure. There are several indications for reconstruction, such as congenital anomalies, mechanical injuries and burns, and a range of methods have been proposed for complete reconstruction of the auricle. Material and methods: A bacterial cellulose membrane, at least 25 mm in thickness, was produced in vitro by Acetobacter xylinum culture. The entire artificial human auricle skeleton was made to scale to allow its implantation into an animal model - Wistar rats. Forty rats were divided into four groups of 10 animals. Each group was assigned a different resection time: 14 days, 30 days, 90 days or 720 days. After each resection, an examination of the artificial skeleton and the tissues surrounding it was conducted. The surgical procedure was based on the Nagata technique. Results: Resection after 14, 30, 90 and 720 days shows progression of the healing process and integration of the artificial skeleton into the animal body. There are no signs of change in the shape or structure of the skeleton. Discussion: Several surgical techniques and biotechnological methods have been developed over the past few years to improve the results of facial reconstruction. Other approaches can be used to create auricle cartilage, based on scaffolds and chondrocytes. Conclusion: My findings indicate that modified bacterial cellulose can be used to form an effective artificial auricle which appears to maintain its shape and elasticity, with no signs of degradation. [ABSTRACT FROM AUTHOR]

Chronic wound healing is currently a severe problem due to its incidence and associated complications. Intensive research is underway on substances that retain their biological activity in the wound microenvironment and stimulate the formation of new blood vessels critical for tissue regeneration. This group includes synthetic compounds with proangiogenic activity. Previously, we identified phosphorothioate analogs of nucleoside 5′-O-monophosphates as multifunctional ligands of P2Y6 and P2Y14 receptors. The effects of a series of unmodified and phosphorothioate nucleotide analogs on the secretion of VEGF from keratinocytes and fibroblasts, as well as their influence on the viability and proliferation of keratinocytes, fibroblasts, and endothelial cells were analyzed. In addition, the expression profiles of genes encoding nucleotide receptors in tested cell models were also investigated. In this study, we defined thymidine 5′-O-monophosphorothioate (TMPS) as a positive regulator of angiogenesis. Preliminary analyses confirmed the proangiogenic potency of TMPS in vivo. [ABSTRACT FROM AUTHOR]

Unlabelled: Modern methods of hernia tension free treatment use very wide range of modern biomaterials. Most of them are used transabdominal. For the best and most convenient treatment of large hernias would be a mesh suitable for intraperitoneal use with low adhesion ability to internal organs.THE AIM OF THE STUDY was to compare three types of intraperitoneal meshes. MATERIAL AND METHODS. The study compared three types of intraperitoneal meshes. Sub-chronic (14 days) and chronic (90 days) macro-and microscopic examination were performed on rats (n = 69). Properties of the polypropylene mesh (PLP), Dynamesh®-iPOM and polypropylene covered with chitosan (PLP+chitosan) were evaluated and compared. RESULTS. It has been shown that the test meshes differ slightly during the healing process., Conclusion: The PLP+chitosan mesh had the best biocompatible features of them all.