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2. Phosphatidylserine-exposing extracellular vesicles in body fluids are an innate defence against apoptotic mimicry viral pathogens

Author

Groß, Rüdiger, Reßin, Hanna, von Maltitz, Pascal, Albers, Dan, Schneider, Laura, Bley, Hanna, Hoffmann, Markus, Cortese, Mirko, Gupta, Dhanu, Deniz, Miriam, Choi, Jae-Yeon, Jansen, Jenny, Preußer, Christian, Seehafer, Kai, Pöhlmann, Stefan, Voelker, Dennis R., Goffinet, Christine, Pogge-von Strandmann, Elke, Bunz, Uwe, Bartenschlager, Ralf, El Andaloussi, Samir, Sparrer, Konstantin M. J., Herker, Eva, Becker, Stephan, Kirchhoff, Frank, Münch, Jan, and Müller, Janis A.

Published
2024
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3. High antibody levels and reduced cellular response in children up to one year after SARS-CoV-2 infection.

Author

Jacobsen, Eva-Maria, Fabricius, Dorit, Class, Magdalena, Topfstedt, Fernando, Lorenzetti, Raquel, Janowska, Iga, Schmidt, Franziska, Staniek, Julian, Zernickel, Maria, Stamminger, Thomas, Dietz, Andrea N, Zellmer, Angela, Hecht, Manuel, Rauch, Peter, Blum, Carmen, Ludwig, Carolin, Jahrsdörfer, Bernd, Schrezenmeier, Hubert, Heeg, Maximilian, Mayer, Benjamin, Seidel, Alina, Groß, Rüdiger, Münch, Jan, Kirchhoff, Frank, Bode, Sebastian FN, Strauss, Gudrun, Renk, Hanna, Elling, Roland, Stich, Maximillian, Voll, Reinhard E, Tönshof, Burkhard, Franz, Axel R, Henneke, Philipp, Debatin, Klaus-Michael, Rizzi, Marta, and Janda, Ales

Subjects
Humans, Immunization, Secondary, Antibody Formation, Adult, Child, Antibodies, Neutralizing, COVID-19, SARS-CoV-2, Immunization, Biodefense, Emerging Infectious Diseases, Infectious Diseases, Prevention, Pediatric, Vaccine Related, 2.1 Biological and endogenous factors, Aetiology, Inflammatory and immune system, Infection, Good Health and Well Being
Abstract

The COVID-19 course and immunity differ in children and adults. We analyzed immune response dynamics in 28 families up to 12 months after mild or asymptomatic infection. Unlike adults, the initial response is plasmablast-driven in children. Four months after infection, children show an enhanced specific antibody response and lower but detectable spike 1 protein (S1)-specific B and T cell responses than their parents. While specific antibodies decline, neutralizing antibody activity and breadth increase in both groups. The frequencies of S1-specific B and T cell responses remain stable. However, in children, one year after infection, an increase in the S1-specific IgA class switch and the expression of CD27 on S1-specific B cells and T cell maturation are observed. These results, together with the enhanced neutralizing potential and breadth of the specific antibodies, suggest a progressive maturation of the S1-specific immune response. Hence, the immune response in children persists over 12 months but dynamically changes in quality, with progressive neutralizing, breadth, and memory maturation. This implies a benefit for booster vaccination in children to consolidate memory formation.

Published
2022

4. Orally bioavailable RORγ/DHODH dual host-targeting small molecules with broad-spectrum antiviral activity

Author

Herrmann, Alexandra, Gege, Christian, Wangen, Christina, Wagner, Sabrina, Kögler, Melanie, Cordsmeier, Arne, Irrgang, Pascal, Ip, Wing-Hang, Weil, Tatjana, Hunszinger, Victoria, Groß, Rüdiger, Heinen, Natalie, Pfaender, Stephanie, Reuter, Sebastian, Klopfleisch, Robert, Uhlig, Nadja, Eberlein, Valentina, Issmail, Leila, Grunwald, Thomas, Hietel, Benjamin, Cynis, Holger, Münch, Jan, Sparrer, Konstantin M.J., Ensser, Armin, Tenbusch, Matthias, Dobner, Thomas, Vitt, Daniel, Kohlhof, Hella, and Hahn, Friedrich

Published
2024
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5. Fatty acid conjugated EPI-X4 derivatives with increased activity and in vivo stability

Author

Harms, Mirja, Haase, André, Rodríguez-Alfonso, Armando, Löffler, Jessica, Almeida-Hernández, Yasser, Ruiz-Blanco, Yasser B., Albers, Dan, Gilg, Andrea, von Bank, Franziska, Zech, Fabian, Groß, Rüdiger, Datta, Moumita, Jaikishan, Janeni, Draphoen, Bastian, Habib, Monica, Ständker, Ludger, Wiese, Sebastian, Lindén, Mika, Winter, Gordon, Rasche, Volker, Beer, Ambros J., Jumaa, Hassan, Abadi, Ashraf H., Kirchhoff, Frank, Busch, Maike, Dünker, Nicole, Sanchez-Garcia, Elsa, and Münch, Jan

Published
2024
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6. The C-terminal 32-mer fragment of hemoglobin alpha is an amyloidogenic peptide with antimicrobial properties

Author

Olari, Lia-Raluca, Bauer, Richard, Gil Miró, Marta, Vogel, Verena, Cortez Rayas, Laura, Groß, Rüdiger, Gilg, Andrea, Klevesath, Raphael, Rodríguez Alfonso, Armando A., Kaygisiz, Kübra, Rupp, Ulrich, Pant, Pradeep, Mieres-Pérez, Joel, Steppe, Lena, Schäffer, Ramona, Rauch-Wirth, Lena, Conzelmann, Carina, Müller, Janis A., Zech, Fabian, Gerbl, Fabian, Bleher, Jana, Preising, Nico, Ständker, Ludger, Wiese, Sebastian, Thal, Dietmar R., Haupt, Christian, Jonker, Hendrik R. A., Wagner, Manfred, Sanchez-Garcia, Elsa, Weil, Tanja, Stenger, Steffen, Fändrich, Marcus, von Einem, Jens, Read, Clarissa, Walther, Paul, Kirchhoff, Frank, Spellerberg, Barbara, and Münch, Jan

Published
2023
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7. Supramolecular Mechanism of Viral Envelope Disruption by Molecular Tweezers

Author

Weil, Tatjana, Groß, Rüdiger, Röcker, Annika, Bravo-Rodriguez, Kenny, Heid, Christian, Sowislok, Andrea, Le, My-Hue, Erwin, Nelli, Dwivedi, Mridula, M., Stephen, Bates, Paul, Wettstein, Lukas, Müller, Janis A, Harms, Mirja, Sparrer, Konstantin, Ruiz-Blanco, Yasser B, Stürzel, Christina M, von Einem, Jens, Lippold, Sina, Read, Clarissa, Walther, Paul, Hebel, Marco, Kreppel, Florian, Klärner, Frank-Gerrit, Bitan, Gal, Ehrmann, Michael, Weil, Tanja, Winter, Roland, Schrader, Thomas, Shorter, James, Sanchez-Garcia, Elsa, and Münch, Jan

Subjects
Engineering, Chemical Sciences, Vaccine Related, Biodefense, Emerging Infectious Diseases, Prevention, Infectious Diseases, Infection, Good Health and Well Being, Acid Phosphatase, Amyloid, Anti-HIV Agents, Antiviral Agents, Arginine, Betacoronavirus, Bridged-Ring Compounds, Cell Membrane, HIV Infections, HIV-1, Humans, Lipids, Lysine, Magnetic Resonance Spectroscopy, Organophosphates, SARS-CoV-2, Seminal Vesicle Secretory Proteins, Structure-Activity Relationship, Viral Envelope Proteins, Zika Virus, General Chemistry, Chemical sciences
Abstract

Broad-spectrum antivirals are powerful weapons against dangerous viruses where no specific therapy exists, as in the case of the ongoing SARS-CoV-2 pandemic. We discovered that a lysine- and arginine-specific supramolecular ligand (CLR01) destroys enveloped viruses, including HIV, Ebola, and Zika virus, and remodels amyloid fibrils in semen that promote viral infection. Yet, it is unknown how CLR01 exerts these two distinct therapeutic activities. Here, we delineate a novel mechanism of antiviral activity by studying the activity of tweezer variants: the "phosphate tweezer" CLR01, a "carboxylate tweezer" CLR05, and a "phosphate clip" PC. Lysine complexation inside the tweezer cavity is needed to antagonize amyloidogenesis and is only achieved by CLR01. Importantly, CLR01 and CLR05 but not PC form closed inclusion complexes with lipid head groups of viral membranes, thereby altering lipid orientation and increasing surface tension. This process disrupts viral envelopes and diminishes infectivity but leaves cellular membranes intact. Consequently, CLR01 and CLR05 display broad antiviral activity against all enveloped viruses tested, including herpesviruses, Measles virus, influenza, and SARS-CoV-2. Based on our mechanistic insights, we potentiated the antiviral, membrane-disrupting activity of CLR01 by introducing aliphatic ester arms into each phosphate group to act as lipid anchors that promote membrane targeting. The most potent ester modifications harbored unbranched C4 units, which engendered tweezers that were approximately one order of magnitude more effective than CLR01 and nontoxic. Thus, we establish the mechanistic basis of viral envelope disruption by specific tweezers and establish a new class of potential broad-spectrum antivirals with enhanced activity.

Published
2020

11. Robust and durable serological response following pediatric SARS-CoV-2 infection

Author

Renk, Hanna, Dulovic, Alex, Seidel, Alina, Becker, Matthias, Fabricius, Dorit, Zernickel, Maria, Junker, Daniel, Groß, Rüdiger, Müller, Janis, Hilger, Alexander, Bode, Sebastian F. N., Fritsch, Linus, Frieh, Pauline, Haddad, Anneke, Görne, Tessa, Remppis, Jonathan, Ganzemueller, Tina, Dietz, Andrea, Huzly, Daniela, Hengel, Hartmut, Kaier, Klaus, Weber, Susanne, Jacobsen, Eva-Maria, Kaiser, Philipp D., Traenkle, Bjoern, Rothbauer, Ulrich, Stich, Maximilian, Tönshoff, Burkhard, Hoffmann, Georg F., Müller, Barbara, Ludwig, Carolin, Jahrsdörfer, Bernd, Schrezenmeier, Hubert, Peter, Andreas, Hörber, Sebastian, Iftner, Thomas, Münch, Jan, Stamminger, Thomas, Groß, Hans-Jürgen, Wolkewitz, Martin, Engel, Corinna, Liu, Weimin, Rizzi, Marta, Hahn, Beatrice H., Henneke, Philipp, Franz, Axel R., Debatin, Klaus-Michael, Schneiderhan-Marra, Nicole, Janda, Ales, and Elling, Roland

Published
2022
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14. Semen inhibits Zika virus infection of cells and tissues from the anogenital region.

Author

Müller, Janis A, Harms, Mirja, Krüger, Franziska, Groß, Rüdiger, Joas, Simone, Hayn, Manuel, Dietz, Andrea N, Lippold, Sina, von Einem, Jens, Schubert, Axel, Michel, Manuela, Mayer, Benjamin, Cortese, Mirko, Jang, Karen S, Sandi-Monroy, Nathallie, Deniz, Miriam, Ebner, Florian, Vapalahti, Olli, Otto, Markus, Bartenschlager, Ralf, Herbeuval, Jean-Philippe, Schmidt-Chanasit, Jonas, Roan, Nadia R, and Münch, Jan

Subjects
Genitalia, Cell Line, Tumor, Vero Cells, Fibroblasts, Semen, Animals, Cercopithecus aethiops, Humans, Sexually Transmitted Diseases, Viral, RNA, Viral, Viral Load, Inhibitory Concentration 50, Virus Replication, Female, Male, Virus Attachment, Primary Cell Culture, Healthy Volunteers, Extracellular Vesicles, Zika Virus, Zika Virus Infection, Chlorocebus aethiops, Cell Line, Tumor, RNA, Viral, Sexually Transmitted Diseases
Abstract

Zika virus (ZIKV) causes severe birth defects and can be transmitted via sexual intercourse. Semen from ZIKV-infected individuals contains high viral loads and may therefore serve as an important vector for virus transmission. Here we analyze the effect of semen on ZIKV infection of cells and tissues derived from the anogenital region. ZIKV replicates in all analyzed cell lines, primary cells, and endometrial or vaginal tissues. However, in the presence of semen, infection by ZIKV and other flaviviruses is potently inhibited. We show that semen prevents ZIKV attachment to target cells, and that an extracellular vesicle preparation from semen is responsible for this anti-ZIKV activity. Our findings suggest that ZIKV transmission is limited by semen. As such, semen appears to serve as a protector against sexual ZIKV transmission, despite the availability of highly susceptible cells in the anogenital tract and high viral loads in this bodily fluid.

Published
2018

15. COVID-eVax, a vaccine candidate encoding the SARS-CoV-2 receptor binding domain, elicits immune responses in animal models of COVID-19

Author

Conforti, Antonella, Marra, Emanuele, Palombo, Fabio, Roscilli, Giuseppe, Ravà, Micol, Fumagalli, Valeria, Muzi, Alessia, Maffei, Mariano, Luberto, Laura, Lione, Lucia, Salvatori, Erika, Compagnone, Mirco, Pinto, Eleonora, Pavoni, Emiliano, Bucci, Federica, Vitagliano, Grazia, Stoppoloni, Daniela, Pacello, Maria Lucrezia, Cappelletti, Manuela, Ferrara, Fabiana Fosca, D’Acunto, Emanuela, Chiarini, Valerio, Arriga, Roberto, Nyska, Abraham, Di Lucia, Pietro, Marotta, Davide, Bono, Elisa, Giustini, Leonardo, Sala, Eleonora, Perucchini, Chiara, Paterson, Jemma, Ryan, Kathryn Ann, Challis, Amy-Rose, Matusali, Giulia, Colavita, Francesca, Caselli, Gianfranco, Criscuolo, Elena, Clementi, Nicola, Mancini, Nicasio, Groß, Rüdiger, Seidel, Alina, Wettstein, Lukas, Münch, Jan, Donnici, Lorena, Conti, Matteo, De Francesco, Raffaele, Kuka, Mirela, Ciliberto, Gennaro, Castilletti, Concetta, Capobianchi, Maria Rosaria, Ippolito, Giuseppe, Guidotti, Luca G., Rovati, Lucio, Iannacone, Matteo, and Aurisicchio, Luigi

Published
2021
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17. Isolation and Characterization of Neutralizing Monoclonal Antibodies from a Large Panel of Murine Antibodies against RBD of the SARS-CoV-2 Spike Protein

Author

D’Acunto, Emanuela, primary, Muzi, Alessia, additional, Marchese, Silvia, additional, Donnici, Lorena, additional, Chiarini, Valerio, additional, Bucci, Federica, additional, Pavoni, Emiliano, additional, Ferrara, Fabiana Fosca, additional, Cappelletti, Manuela, additional, Arriga, Roberto, additional, Serrao, Silvia Maria, additional, Peluzzi, Valentina, additional, Principato, Eugenia, additional, Compagnone, Mirco, additional, Pinto, Eleonora, additional, Luberto, Laura, additional, Stoppoloni, Daniela, additional, Lahm, Armin, additional, Groß, Rüdiger, additional, Seidel, Alina, additional, Wettstein, Lukas, additional, Münch, Jan, additional, Goodhead, Andrew, additional, Parisot, Judicael, additional, De Francesco, Raffaele, additional, Ciliberto, Gennaro, additional, Marra, Emanuele, additional, Aurisicchio, Luigi, additional, and Roscilli, Giuseppe, additional

Published
2024
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18. SARS-CoV-2 infects and replicates in cells of the human endocrine and exocrine pancreas

Author

Müller, Janis A., Groß, Rüdiger, Conzelmann, Carina, Krüger, Jana, Merle, Uta, Steinhart, Johannes, Weil, Tatjana, Koepke, Lennart, Bozzo, Caterina Prelli, Read, Clarissa, Fois, Giorgio, Eiseler, Tim, Gehrmann, Julia, van Vuuren, Joanne, Wessbecher, Isabel M., Frick, Manfred, Costa, Ivan G., Breunig, Markus, Grüner, Beate, Peters, Lynn, Schuster, Michael, Liebau, Stefan, Seufferlein, Thomas, Stenger, Steffen, Stenzinger, Albrecht, MacDonald, Patrick E., Kirchhoff, Frank, Sparrer, Konstantin M. J., Walther, Paul, Lickert, Heiko, Barth, Thomas F. E., Wagner, Martin, Münch, Jan, Heller, Sandra, and Kleger, Alexander

Published
2021
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19. Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection

Author

Wettstein, Lukas, Weil, Tatjana, Conzelmann, Carina, Müller, Janis A., Groß, Rüdiger, Hirschenberger, Maximilian, Seidel, Alina, Klute, Susanne, Zech, Fabian, Prelli Bozzo, Caterina, Preising, Nico, Fois, Giorgio, Lochbaum, Robin, Knaff, Philip Maximilian, Mailänder, Volker, Ständker, Ludger, Thal, Dietmar Rudolf, Schumann, Christian, Stenger, Steffen, Kleger, Alexander, Lochnit, Günter, Mayer, Benjamin, Ruiz-Blanco, Yasser B., Hoffmann, Markus, Sparrer, Konstantin M. J., Pöhlmann, Stefan, Sanchez-Garcia, Elsa, Kirchhoff, Frank, Frick, Manfred, and Münch, Jan

Published
2021
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20. IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro

Author

Prelli Bozzo, Caterina, Nchioua, Rayhane, Volcic, Meta, Koepke, Lennart, Krüger, Jana, Schütz, Desiree, Heller, Sandra, Stürzel, Christina M., Kmiec, Dorota, Conzelmann, Carina, Müller, Janis, Zech, Fabian, Braun, Elisabeth, Groß, Rüdiger, Wettstein, Lukas, Weil, Tatjana, Weiß, Johanna, Diofano, Federica, Rodríguez Alfonso, Armando A., Wiese, Sebastian, Sauter, Daniel, Münch, Jan, Goffinet, Christine, Catanese, Alberto, Schön, Michael, Boeckers, Tobias M., Stenger, Steffen, Sato, Kei, Just, Steffen, Kleger, Alexander, Sparrer, Konstantin M. J., and Kirchhoff, Frank

Published
2021
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21. Lipid A in outer membrane vesicles shields bacteria from polymyxins.

Author

Burt, Marie, Angelidou, Georgia, Mais, Christopher Nils, Preußer, Christian, Glatter, Timo, Heimerl, Thomas, Groß, Rüdiger, Serrania, Javier, Boosarpu, Gowtham, Pogge von Strandmann, Elke, Müller, Janis A., Bange, Gert, Becker, Anke, Lehmann, Mareike, Jonigk, Danny, Neubert, Lavinia, Freitag, Hinrich, Paczia, Nicole, Schmeck, Bernd, and Jung, Anna Lena

Abstract

The continuous emergence of multidrug‐resistant bacterial pathogens poses a major global healthcare challenge, with Klebsiella pneumoniae being a prominent threat. We conducted a comprehensive study on K. pneumoniae's antibiotic resistance mechanisms, focusing on outer membrane vesicles (OMVs) and polymyxin, a last‐resort antibiotic. Our research demonstrates that OMVs protect bacteria from polymyxins. OMVs derived from Polymyxin B (PB)‐stressed K. pneumoniae exhibited heightened protective efficacy due to increased vesiculation, compared to OMVs from unstressed Klebsiella. OMVs also shield bacteria from different bacterial families. This was validated ex vivo and in vivo using precision cut lung slices (PCLS) and Galleria mellonella. In all models, OMVs protected K. pneumoniae from PB and reduced the associated stress response on protein level. We observed significant changes in the lipid composition of OMVs upon PB treatment, affecting their binding capacity to PB. The altered binding capacity of single OMVs from PB stressed K. pneumoniae could be linked to a reduction in the lipid A amount of their released vesicles. Although the amount of lipid A per vesicle is reduced, the overall increase in the number of vesicles results in an increased protection because the sum of lipid A and therefore PB binding sites have increased. This unravels the mechanism of the altered PB protective efficacy of OMVs from PB stressed K. pneumoniae compared to control OMVs. The lipid A‐dependent protective effect against PB was confirmed in vitro using artificial vesicles. Moreover, artificial vesicles successfully protected Klebsiella from PB ex vivo and in vivo. The findings indicate that OMVs act as protective shields for bacteria by binding to polymyxins, effectively serving as decoys and preventing antibiotic interaction with the cell surface. Our findings provide valuable insights into the mechanisms underlying antibiotic cross‐protection and offer potential avenues for the development of novel therapeutic interventions to address the escalating threat of multidrug‐resistant bacterial infections. [ABSTRACT FROM AUTHOR]

Published
2024
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22. Isolation and Characterization of Neutralizing Monoclonal Antibodies from a Large Panel of Murine Antibodies against RBD of the SARS-CoV-2 Spike Protein.

Author

D'Acunto, Emanuela, Muzi, Alessia, Marchese, Silvia, Donnici, Lorena, Chiarini, Valerio, Bucci, Federica, Pavoni, Emiliano, Ferrara, Fabiana Fosca, Cappelletti, Manuela, Arriga, Roberto, Serrao, Silvia Maria, Peluzzi, Valentina, Principato, Eugenia, Compagnone, Mirco, Pinto, Eleonora, Luberto, Laura, Stoppoloni, Daniela, Lahm, Armin, Groß, Rüdiger, and Seidel, Alina

Subjects
MONOCLONAL antibodies, SARS-CoV-2 Omicron variant, SARS-CoV-2, RECOMBINANT proteins, IMMUNOGLOBULINS, HUMORAL immunity
Abstract

The COVID-19 pandemic, once a global crisis, is now largely under control, a testament to the extraordinary global efforts involving vaccination and public health measures. However, the relentless evolution of SARS-CoV-2, leading to the emergence of new variants, continues to underscore the importance of remaining vigilant and adaptable. Monoclonal antibodies (mAbs) have stood out as a powerful and immediate therapeutic response to COVID-19. Despite the success of mAbs, the evolution of SARS-CoV-2 continues to pose challenges and the available antibodies are no longer effective. New variants require the ongoing development of effective antibodies. In the present study, we describe the generation and characterization of neutralizing mAbs against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein by combining plasmid DNA and recombinant protein vaccination. By integrating genetic immunization for rapid antibody production and the potent immune stimulation enabled by protein vaccination, we produced a rich pool of antibodies, each with unique binding and neutralizing specificities, tested with the ELISA, BLI and FACS assays and the pseudovirus assay, respectively. Here, we present a panel of mAbs effective against the SARS-CoV-2 variants up to Omicron BA.1 and BA.5, with the flexibility to target emerging variants. This approach ensures the preparedness principle is in place to address SARS-CoV-2 actual and future infections. [ABSTRACT FROM AUTHOR]

Published
2024
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23. Heterologous DNA-prime/protein-boost immunization with a monomeric SARS-CoV-2 spike antigen redundantizes the trimeric receptor-binding domain structure to induce neutralizing antibodies in old mice

Author

Pflumm, Dominik, primary, Seidel, Alina, additional, Klein, Fabrice, additional, Groß, Rüdiger, additional, Krutzke, Lea, additional, Kochanek, Stefan, additional, Kroschel, Joris, additional, Münch, Jan, additional, Stifter, Katja, additional, and Schirmbeck, Reinhold, additional

Published
2023
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24. Spermine and spermidine bind CXCR4 and inhibit CXCR4- but not CCR5-tropic HIV-1 infection

Author

Harms, Mirja, primary, Smith, Nikaïa, additional, Han, Mingyu, additional, Groß, Rüdiger, additional, von Maltitz, Pascal, additional, Stürzel, Christina, additional, Ruiz-Blanco, Yasser B., additional, Almeida-Hernández, Yasser, additional, Rodriguez-Alfonso, Armando, additional, Cathelin, Dominique, additional, Caspar, Birgit, additional, Tahar, Bouceba, additional, Sayettat, Sophie, additional, Bekaddour, Nassima, additional, Vanshylla, Kanika, additional, Kleipass, Franziska, additional, Wiese, Sebastian, additional, Ständker, Ludger, additional, Klein, Florian, additional, Lagane, Bernard, additional, Boonen, Arnaud, additional, Schols, Dominique, additional, Benichou, Serge, additional, Sanchez-Garcia, Elsa, additional, Herbeuval, Jean-Philippe, additional, and Münch, Jan, additional

Published
2023
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26. Serum neutralizing capacity and T-cell response against the omicron BA.1 variant in seropositive children and their parents one year after SARS-CoV-2 infection

Author

Seidel, Alina, primary, Jacobsen, Eva-Maria, additional, Fabricius, Dorit, additional, Class, Magdalena, additional, Zernickel, Maria, additional, Blum, Carmen, additional, Conzelmann, Carina, additional, Weil, Tatjana, additional, Groß, Rüdiger, additional, Bode, Sebastian F. N., additional, Renk, Hanna, additional, Elling, Roland, additional, Stich, Maximillian, additional, Kirchhoff, Frank, additional, Debatin, Klaus-Michael, additional, Münch, Jan, additional, and Janda, Aleš, additional

Published
2023
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27. Extracellular vesicles in human body fluids as innate antiviral factors

Author

Groß, Rüdiger Martin, Münch, Jan, Barth, Holger, Roan, Nadia, Margolis, Leonid, and Trkola, Alexandra

Subjects
Virology, Liposomes, Angeborene Immunität, ddc:610, Extracellular vesicles, Exosomes, Extrazellulärraum, DDC 610 / Medicine & health, Microvesicles, Immunity, Innate, Zika virus
Abstract

Following Zika virus (ZIKV) infection, RNA and infectious virus are detectable and persistent in multiple body fluids, including semen and saliva, yet there are only isolated cases of sexual transmission and no confirmed cases of transmission by deep kissing, e.g. by travelers returning from endemic regions. Previously, cells and tissues of the anogenital tract were found to be permissive to ZIKV infection, but semen and seminal plasma potently blocked infection by preventing attachment of virions. Highly abundant extracellular vesicles (EVs), lipid-enveloped nanoparticles, were identified as the responsible factor in seminal plasma, however, the exact mechanism underlying anti-ZIKV activity was unclear. Here, these findings are extended to EVs from other body fluids and a major underlying mechanism is revealed. Initially, saliva was found to inhibit infection by ZIKV in vitro in a similar manner as observed for seminal plasma. Accordingly, enriched and purified salivary EVs also exerted competitive inhibition of ZIKV attachment and infection. Inhibition of ZIKV infection was conserved across multiple donors and also exhibited on primary gingival fibroblasts as potential target cells for oral ZIKV transmission. In contrast to ZIKV as a virus detectible in saliva but not transmissible via the oral cavity, SARS-CoV-2, which is detectible in saliva and readily transmissible in presence of saliva-containing respiratory droplets, was not inhibited by either saliva or purified EVs, suggesting specificity of the underlying mechanism. Flaviviruses such as ZIKV use virion-associated phosphatidylserine (PS) to engage cellular receptors, which physiologically recognize material from apoptotic cells for phagocytosis. This process is termed viral apoptotic mimicry (VAM). In this study, EVs purified from body fluids using tangential flow filtration and bind-elute size exclusion chromatography were found to also contain high levels of PS using lipidomics, e.g. ~ 60 mol% of phospholipids in EVs from urine and seminal plasma and ~ 20-30 mol% in EVs from saliva, breast milk or cell culture supernatant, with variable levels of surface exposure determined by flow cytometry. All of these EVs inhibited ZIKV infection in vitro. Purified EVs from seminal plasma also potently inhibited ZIKV infection of ex vivo vaginal tissue, thus confirming results obtained in cell lines in a tissue relevant for sexual virus transmission. Notably, usage of VAM is widespread amongst viruses of different families and indeed, related flaviviruses WNV and DENV were also inhibited by PS-containing EVs from seminal plasma and/or synthetic PS-containing liposomes, as was filovirus EBOV, but not PS-independent herpesviruses HSV-1, -2 and HCMV or SARS-CoV-2. The specific importance of PS for ZIKV infection was confirmed by enzymatic PS modification (cleavage or conversion to phosphatidylethanolamine) of virions, which abrogated infection of ZIKV, but not HIV-1, HSV-1 or -2. Given the abundance of PS-containing EVs in various body fluids, outnumbering virions by several orders of magnitude, competitive attachment inhibition of viruses may represent a novel aspect of innate immunity, preventing transmission of VAM-using viruses. Beyond innate immunity, the observed antiviral mechanism of VAM-competition by EVs or synthetic vesicles may be exploited for therapeutic purposes. Towards this goal, highly concentrated PS-containing liposomes were tested for in vivo tolerability in zebrafish embryos and mice and no adverse effects were observed. Development of bio-engineered EVs or synthetic, PS-containing vesicles may thus be a perspective for treatment or prevention of infections by VAM-using viruses where no specific antivirals exist to date.

Published
2022

28. Advanced Molecular Tweezers with Lipid Anchors against SARS-CoV-2 and Other Respiratory Viruses

Author

Weil, Tatjana, primary, Kirupakaran, Abbna, additional, Le, My-Hue, additional, Rebmann, Philipp, additional, Mieres-Perez, Joel, additional, Issmail, Leila, additional, Conzelmann, Carina, additional, Müller, Janis A., additional, Rauch, Lena, additional, Gilg, Andrea, additional, Wettstein, Lukas, additional, Groß, Rüdiger, additional, Read, Clarissa, additional, Bergner, Tim, additional, Pålsson, Sandra Axberg, additional, Uhlig, Nadja, additional, Eberlein, Valentina, additional, Wöll, Heike, additional, Klärner, Frank-Gerrit, additional, Stenger, Steffen, additional, Kümmerer, Beate M., additional, Streeck, Hendrik, additional, Fois, Giorgio, additional, Frick, Manfred, additional, Braubach, Peter, additional, Spetz, Anna-Lena, additional, Grunwald, Thomas, additional, Shorter, James, additional, Sanchez-Garcia, Elsa, additional, Schrader, Thomas, additional, and Münch, Jan, additional

Published
2022
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29. Actin cytoskeleton remodeling primes RIG-I-like receptor activation

Author

Acharya, Dhiraj, primary, Reis, Rebecca, additional, Volcic, Meta, additional, Liu, GuanQun, additional, Wang, May K., additional, Chia, Bing Shao, additional, Nchioua, Rayhane, additional, Groß, Rüdiger, additional, Münch, Jan, additional, Kirchhoff, Frank, additional, Sparrer, Konstantin M.J., additional, and Gack, Michaela U., additional

Published
2022
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30. Utilization of aminoguanidine prevents cytotoxic effects of semen

Author

Harms, Mirja, von Maltitz, Pascal, Groß, Rüdiger, Mayer, Benjamin, Deniz, Miriam, Müller, Janis, Münch, Jan, and Baldi, Elisabetta

Subjects
Serum, Polyamine, Spermidine, Guanidines, Catalysis, Inorganic Chemistry, Semen, Sperma, Polyamines, Humans, ddc:610, seminal fluid, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, Gerontamin, cytotoxicity, polyamines, spermine, Organic Chemistry, Serum Albumin, Bovine, General Medicine, Computer Science Applications, Cytotoxizität, Spermine, Oxidoreductases, DDC 610 / Medicine & health
Abstract

Studies of human semen in cell or tissue culture are hampered by the high cytotoxic activity of this body fluid. The components responsible for the cell damaging activity of semen are amine oxidases, which convert abundant polyamines, such as spermine or spermidine in seminal plasma into toxic intermediates. Amine oxidases are naturally present at low concentrations in seminal plasma and at high concentrations in fetal calf serum, a commonly used cell culture supplement. Here, we show that, in the presence of fetal calf serum, seminal plasma, as well as the polyamines spermine and spermidine, are highly cytotoxic to immortalized cells, primary blood mononuclear cells, and vaginal tissue. Thus, experiments investigating the effect of polyamines and seminal plasma on cellular functions should be performed with great caution, considering the confounding cytotoxic effects. The addition of the amine oxidase inhibitor aminoguanidine to fetal calf serum and/or the utilization of serum-free medium greatly reduced this serum-induced cytotoxicity of polyamines and seminal plasma in cell lines, primary cells, and tissues and, thus, should be implemented in all future studies analyzing the role of polyamines and semen on cellular functions., publishedVersion

Published
2022

32. BNT162b2 booster after heterologous prime-boost vaccination induces potent neutralizing antibodies and T cell reactivity against SARS-CoV-2 Omicron BA.1 in young adults

Author

Seidel, Alina, primary, Zanoni, Michelle, additional, Groß, Rüdiger, additional, Krnavek, Daniela, additional, Erdemci-Evin, Sümeyye, additional, von Maltitz, Pascal, additional, Albers, Dan P. J., additional, Conzelmann, Carina, additional, Liu, Sichen, additional, Weil, Tatjana, additional, Mayer, Benjamin, additional, Hoffmann, Markus, additional, Pöhlmann, Stefan, additional, Beil, Alexandra, additional, Kroschel, Joris, additional, Kirchhoff, Frank, additional, Münch, Jan, additional, and Müller, Janis A., additional

Published
2022
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33. Advanced Molecular Tweezers with Lipid Anchors against SARS-CoV-2 and Other Respiratory Viruses

Author

Weil, Tatjana, Kirupakaran, Abbna, Le, My-Hue, Rebmann, Philipp, Mieres-Perez, Joel, Issmail, Leila, Conzelmann, Carina, Müller, Janis A., Rauch, Lena, Gilg, Andrea, Wettstein, Lukas, Groß, Rüdiger, Read, Clarissa, Bergner, Tim, Axberg Pålsson, Sandra, Uhlig, Nadja, Eberlein, Valentina, Wöll, Heike, Klärner, Frank-Gerrit, Stenger, Steffen, Kümmerer, Beate M., Streeck, Hendrik, Fois, Giorgio, Frick, Manfred, Braubach, Peter, Spetz, Anna-Lena, Grunwald, Thomas, Shorter, James, Sanchez-Garcia, Elsa, Schrader, Thomas, Münch, Jan, Weil, Tatjana, Kirupakaran, Abbna, Le, My-Hue, Rebmann, Philipp, Mieres-Perez, Joel, Issmail, Leila, Conzelmann, Carina, Müller, Janis A., Rauch, Lena, Gilg, Andrea, Wettstein, Lukas, Groß, Rüdiger, Read, Clarissa, Bergner, Tim, Axberg Pålsson, Sandra, Uhlig, Nadja, Eberlein, Valentina, Wöll, Heike, Klärner, Frank-Gerrit, Stenger, Steffen, Kümmerer, Beate M., Streeck, Hendrik, Fois, Giorgio, Frick, Manfred, Braubach, Peter, Spetz, Anna-Lena, Grunwald, Thomas, Shorter, James, Sanchez-Garcia, Elsa, Schrader, Thomas, and Münch, Jan

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 presents a global health emergency. Therapeutic options against SARS-CoV-2 are still very limited but urgently required. Molecular tweezers are supramolecular agents that destabilize the envelope of viruses resulting in a loss of viral infectivity. Here, we show that first-generation tweezers, CLR01 and CLR05, disrupt the SARS-CoV-2 envelope and abrogate viral infectivity. To increase the antiviral activity, a series of 34 advanced molecular tweezers were synthesized by insertion of aliphatic or aromatic ester groups on the phosphate moieties of the parent molecule CLR01. A structure-activity relationship study enabled the identification of tweezers with a markedly enhanced ability to destroy lipid bilayers and to suppress SARS-CoV-2 infection. Selected tweezer derivatives retain activity in airway mucus and inactivate the SARS-CoV-2 wildtype and variants of concern as well as respiratory syncytial, influenza, and measles viruses. Moreover, inhibitory activity of advanced tweezers against respiratory syncytial virus and SARS-CoV-2 was confirmed in mice. Thus, potentiated tweezers are broad-spectrum antiviral agents with great prospects for clinical development to combat highly pathogenic viruses.

Published
2022
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34. Macromolecular Viral Entry Inhibitors as Broad-Spectrum First-Line Antivirals with Activity against SARS-CoV-2

Author

Groß, Rüdiger, Dias Loiola, Lívia Mesquita, Issmail, Leila, Uhlig, Nadja, Eberlein, Valentina, Conzelmann, Carina, Olari, Lia-Raluca, Rauch, Lena, Lawrenz, Jan, Weil, Tatjana, Müller, Janis A., Cardoso, Mateus Borba, Gilg, Andrea, Larsson, Olivia, Höglund, Urban, Axberg Pålsson, Sandra, Tvilum, Anna Selch, Løvschall, Kaja Borup, Kristensen, Maria M., Spetz, Anna-Lena, Hontonnou, Fortune, Galloux, Marie, Grunwald, Thomas, Zelikin, Alexander N., Münch, Jan, Groß, Rüdiger, Dias Loiola, Lívia Mesquita, Issmail, Leila, Uhlig, Nadja, Eberlein, Valentina, Conzelmann, Carina, Olari, Lia-Raluca, Rauch, Lena, Lawrenz, Jan, Weil, Tatjana, Müller, Janis A., Cardoso, Mateus Borba, Gilg, Andrea, Larsson, Olivia, Höglund, Urban, Axberg Pålsson, Sandra, Tvilum, Anna Selch, Løvschall, Kaja Borup, Kristensen, Maria M., Spetz, Anna-Lena, Hontonnou, Fortune, Galloux, Marie, Grunwald, Thomas, Zelikin, Alexander N., and Münch, Jan

Abstract

Inhibitors of viral cell entry based on poly(styrene sulfonate) and its core–shell nanoformulations based on gold nanoparticles are investigated against a panel of viruses, including clinical isolates of SARS-CoV-2. Macromolecular inhibitors are shown to exhibit the highly sought-after broad-spectrum antiviral activity, which covers most analyzed enveloped viruses and all of the variants of concern for SARS-CoV-2 tested. The inhibitory activity is quantified in vitro in appropriate cell culture models and for respiratory viral pathogens (respiratory syncytial virus and SARS-CoV-2) in mice. Results of this study comprise a significant step along the translational path of macromolecular inhibitors of virus cell entry, specifically against enveloped respiratory viruses.

Published
2022
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35. Airborne Pathogen Detection in Fine Aerosol Exhaled Breath Condensates

Author

Henderson, John, primary, Mantso, Theodora, additional, Ali, Saqib, additional, Groß, Rüdiger, additional, Müller, Janis A., additional, Wilkinson, Amie, additional, Shah, Kavit, additional, Usher, Louise, additional, Auld, Beth, additional, Nelson, Andrew, additional, Cheung, William, additional, Namdeo, Anil, additional, Combrinck, Madeleine, additional, Hackney, Phil, additional, Turgul, Volkan, additional, Jahaj, Edison, additional, Athanasiou, Nikolaos, additional, Nikolouzakis, Taxiarchis, additional, Almeida, Pedro J., additional, Rokka, Chrysa, additional, Queiroz, Daniel C., additional, Wright, Edward, additional, Zafiropoulos, Alexandros, additional, Kale, Izzet, additional, Smith, Darren, additional, Kofteridis, Diamantis P., additional, Tsatsakis, Aristides, additional, Münch, Jan, additional, Katsaounou, Paraskevi A., additional, Kotanidou, Anastasia, additional, Lagiou, Pagona, additional, Magiorkinis, Gkikas, additional, Aquiar, Renato S, additional, Teixeira, Mauro M., additional, and Moschos, Sterghios A., additional

Published
2022
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36. Macromolecular Viral Entry Inhibitors as Broad‐Spectrum First‐Line Antivirals with Activity against SARS‐CoV‐2

Author

Groß, Rüdiger, primary, Dias Loiola, Lívia Mesquita, additional, Issmail, Leila, additional, Uhlig, Nadja, additional, Eberlein, Valentina, additional, Conzelmann, Carina, additional, Olari, Lia‐Raluca, additional, Rauch, Lena, additional, Lawrenz, Jan, additional, Weil, Tatjana, additional, Müller, Janis A., additional, Cardoso, Mateus Borba, additional, Gilg, Andrea, additional, Larsson, Olivia, additional, Höglund, Urban, additional, Pålsson, Sandra Axberg, additional, Tvilum, Anna Selch, additional, Løvschall, Kaja Borup, additional, Kristensen, Maria M., additional, Spetz, Anna‐Lena, additional, Hontonnou, Fortune, additional, Galloux, Marie, additional, Grunwald, Thomas, additional, Zelikin, Alexander N., additional, and Münch, Jan, additional

Published
2022
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38. High antibody and reduced cellular response in children up to one year after SARS-CoV-2 infection

Author

Jacobsen, Eva-Maria, primary, Fabricius, Dorit, additional, Class, Magdalena, additional, Topfstedt, Fernando, additional, Lorenzetti, Raquel, additional, Janowska, Iga, additional, Schmidt, Franziska, additional, Staniek, Julian, additional, Zernickel, Maria, additional, Stamminger, Thomas, additional, Dietz, Andrea, additional, Zellmer, Angela, additional, Hecht, Manuel, additional, Rauch, Peter, additional, Blum, Carmen, additional, Ludwig, Carolin, additional, Jahrsdörfer, Bernd, additional, Schrezenmeier, Hubert, additional, Heeg, Maximilian, additional, Mayer, Benjamin, additional, Seidel, Alina, additional, Groß, Rüdiger, additional, Münch, Jan, additional, Bode, Sebastian, additional, Strauss, Gudrun, additional, Renk, Hanna, additional, Elling, Roland, additional, Stich, Maximilian, additional, Voll, Reinhard, additional, Tönshoff, Burkhard, additional, Franz, Axel, additional, Henneke, Philipp, additional, Debatin, Klaus-Michael, additional, Rizzi, Marta, additional, and Janda, Aleš, additional

Published
2022
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40. Heterologous ChAdOx1 nCoV-19 and BNT162b2 prime-boost vaccination elicits potent neutralizing antibody responses and T cell reactivity against prevalent SARS-CoV-2 variants

Author

Groß, Rüdiger, primary, Zanoni, Michelle, additional, Seidel, Alina, additional, Conzelmann, Carina, additional, Gilg, Andrea, additional, Krnavek, Daniela, additional, Erdemci-Evin, Sümeyye, additional, Mayer, Benjamin, additional, Hoffmann, Markus, additional, Pöhlmann, Stefan, additional, Liu, Weimin, additional, Hahn, Beatrice H., additional, Beil, Alexandra, additional, Kroschel, Joris, additional, Jahrsdörfer, Bernd, additional, Schrezenmeier, Hubert, additional, Kirchhoff, Frank, additional, Münch, Jan, additional, and Müller, Janis A., additional

Published
2022
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41. COVID-eVax, an electroporated DNA vaccine candidate encoding the SARS-CoV-2 RBD, elicits protective responses in animal models

Author

Conforti, Antonella, primary, Marra, Emanuele, additional, Palombo, Fabio, additional, Roscilli, Giuseppe, additional, Ravà, Micol, additional, Fumagalli, Valeria, additional, Muzi, Alessia, additional, Maffei, Mariano, additional, Luberto, Laura, additional, Lione, Lucia, additional, Salvatori, Erika, additional, Compagnone, Mirco, additional, Pinto, Eleonora, additional, Pavoni, Emiliano, additional, Bucci, Federica, additional, Vitagliano, Grazia, additional, Stoppoloni, Daniela, additional, Pacello, Maria Lucrezia, additional, Cappelletti, Manuela, additional, Ferrara, Fabiana Fosca, additional, D’Acunto, Emanuela, additional, Chiarini, Valerio, additional, Arriga, Roberto, additional, Nyska, Abraham, additional, Di Lucia, Pietro, additional, Marotta, Davide, additional, Bono, Elisa, additional, Giustini, Leonardo, additional, Sala, Eleonora, additional, Perucchini, Chiara, additional, Paterson, Jemma, additional, Ryan, Kathryn Ann, additional, Challis, Amy-Rose, additional, Matusali, Giulia, additional, Colavita, Francesca, additional, Caselli, Gianfranco, additional, Criscuolo, Elena, additional, Clementi, Nicola, additional, Mancini, Nicasio, additional, Groß, Rüdiger, additional, Seidel, Alina, additional, Wettstein, Lukas, additional, Münch, Jan, additional, Donnici, Lorena, additional, Conti, Matteo, additional, De Francesco, Raffaele, additional, Kuka, Mirela, additional, Ciliberto, Gennaro, additional, Castilletti, Concetta, additional, Capobianchi, Maria Rosaria, additional, Ippolito, Giuseppe, additional, Guidotti, Luca G., additional, Rovati, Lucio, additional, Iannacone, Matteo, additional, and Aurisicchio, Luigi, additional

Published
2022
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43. Virucidal activity of SARS-CoV-2 rapid antigen extraction buffers

Author

Conzelmann, Carina, Weil, Tatjana, Olari, Lia-Raluca, Gilg, Andrea, Rauch, Lena, Albers, Dan P.J., Groß, Rüdiger, Müller, Janis A., and Münch, Jan

Subjects
Infectious Diseases, SARS-CoV-2, SARS-CoV-2 rapid antigen test, Virology, Point-of-Care Systems, Antigen extraction buffer, COVID-19, Humans, Immunologic Tests, Point-of-care devices, Assay safety, Virucidal buffer, Article
Abstract

Since diagnostic sampling material must be considered as infectious, we evaluated whether extraction buffers of SARS-CoV-2 rapid antigen test kits may inactivate SARS-CoV-2. Of concern, seven of nine tested buffers lacked potent virucidal activity. To reduce risk of infection during assay performance, virucidal antigen extraction buffers that efficiently inactivate virus should replace the extraction buffers in these commercially available point-of-care devices.

Published
2021

46. Typically asymptomatic but with robust antibody formation: Children’s unique humoral immune response to SARS-CoV-2

Author

Renk, Hanna, primary, Dulovic, Alex, additional, Becker, Matthias, additional, Fabricius, Dorit, additional, Zernickel, Maria, additional, Junker, Daniel, additional, Seidel, Alina, additional, Groß, Rüdiger, additional, Hilger, Alexander, additional, Bode, Sebastian, additional, Fritsch, Linus, additional, Frieh, Pauline, additional, Haddad, Anneke, additional, Görne, Tessa, additional, Remppis, Jonathan, additional, Ganzemueller, Tina, additional, Dietz, Andrea, additional, Huzly, Daniela, additional, Hengel, Hartmut, additional, Kaier, Klaus, additional, Weber, Susanne, additional, Jacobsen, Eva-Maria, additional, Kaiser, Philipp D., additional, Traenkle, Bjoern, additional, Rothbauer, Ulrich, additional, Stich, Maximilian, additional, Tönshoff, Burkhard, additional, Hoffmann, Georg F., additional, Müller, Barbara, additional, Ludwig, Carolin, additional, Jahrsdörfer, Bernd, additional, Schrezenmeier, Hubert, additional, Peter, Andreas, additional, Hörber, Sebastian, additional, Iftner, Thomas, additional, Münch, Jan, additional, Stamminger, Thomas, additional, Groß, Hans-Jürgen, additional, Wolkewitz, Martin, additional, Engel, Corinna, additional, Rizzi, Marta, additional, Henneke, Philipp, additional, Franz, Axel R., additional, Debatin, Klaus-Michael, additional, Schneiderhan-Marra, Nicole, additional, Janda, Ales, additional, and Elling, Roland, additional

Published
2021
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48. COVID-eVax, an electroporated plasmid DNA vaccine candidate encoding the SARS-CoV-2 Receptor Binding Domain, elicits protective immune responses in animal models of COVID-19

Author

Conforti, Antonella, primary, Marra, Emanuele, additional, Palombo, Fabio, additional, Roscilli, Giuseppe, additional, Ravà, Micol, additional, Fumagalli, Valeria, additional, Muzi, Alessia, additional, Maffei, Mariano, additional, Luberto, Laura, additional, Lione, Lucia, additional, Salvatori, Erika, additional, Compagnone, Mirco, additional, Pinto, Eleonora, additional, Pavoni, Emiliano, additional, Bucci, Federica, additional, Vitagliano, Grazia, additional, Stoppoloni, Daniela, additional, Pacello, Maria Lucrezia, additional, Cappelletti, Manuela, additional, Ferrara, Fabiana Fosca, additional, D’Acunto, Emanuela, additional, Chiarini, Valerio, additional, Arriga, Roberto, additional, Nyska, Abraham, additional, Lucia, Pietro Di, additional, Marotta, Davide, additional, Bono, Elisa, additional, Giustini, Leonardo, additional, Sala, Eleonora, additional, Perucchini, Chiara, additional, Paterson, Jemma, additional, Ryan, Kathryn Ann, additional, Challis, Amy-Rose, additional, Matusali, Giulia, additional, Colavita, Francesca, additional, Caselli, Gianfranco, additional, Criscuolo, Elena, additional, Clementi, Nicola, additional, Mancini, Nicasio, additional, Groß, Rüdiger, additional, Seidel, Alina, additional, Wettstein, Lukas, additional, Münch, Jan, additional, Donnici, Lorena, additional, Conti, Matteo, additional, Francesco, Raffaele De, additional, Kuka, Mirela, additional, Ciliberto, Gennaro, additional, Castilletti, Concetta, additional, Capobianchi, Maria Rosaria, additional, Ippolito, Giuseppe, additional, Guidotti, Luca G., additional, Rovati, Lucio, additional, Iannacone, Matteo, additional, and Aurisicchio, Luigi, additional

Published
2021
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49. Characterization of the SARS-CoV-2 Neutralization Potential of COVID-19–Convalescent Donors

Author

Jahrsdörfer, Bernd, primary, Groß, Rüdiger, additional, Seidel, Alina, additional, Wettstein, Lukas, additional, Ludwig, Carolin, additional, Schwarz, Tatjana, additional, Körper, Sixten, additional, Rojewski, Markus, additional, Lotfi, Ramin, additional, Weinstock, Christoph, additional, Seifried, Erhard, additional, Corman, Victor Max, additional, Drosten, Christian, additional, Münch, Jan, additional, and Schrezenmeier, Hubert, additional

Published
2021
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50. Heterologous ChAdOx1 nCoV-19 and BNT162b2 prime-boost vaccination elicits potent neutralizing antibody responses and T cell reactivity

Author

Groß, Rüdiger, primary, Zanoni, Michelle, additional, Seidel, Alina, additional, Conzelmann, Carina, additional, Gilg, Andrea, additional, Krnavek, Daniela, additional, Erdemci-Evin, Sümeyye, additional, Mayer, Benjamin, additional, Hoffmann, Markus, additional, Pöhlmann, Stefan, additional, Beil, Alexandra, additional, Kroschel, Joris, additional, Jahrsdörfer, Bernd, additional, Schrezenmeier, Hubert, additional, Kirchhoff, Frank, additional, Münch, Jan, additional, and Müller, Janis A., additional

Published
2021
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