1. Development and validation of a sensitive LC-MS/MS assay of GT-14, a novel Gα i 2 inhibitor, in rat plasma, and its application in pharmacokinetic study.
- Author
-
Sarkar M, Ma J, Tapadar S, Caggia S, Oyelere AK, Khan SA, and Xie H
- Subjects
- Animals, Male, Rats, Liquid Chromatography-Mass Spectrometry, Protein Binding, Rats, Sprague-Dawley, Reproducibility of Results, Spectrometry, Mass, Electrospray Ionization methods, Tandem Mass Spectrometry methods, Griseofulvin pharmacokinetics, Griseofulvin blood
- Abstract
A sensitive and selective LC-MS/MS method was developed and validated for the quantitation of a novel Gα
i 2 inhibitor, GT-14, in rat plasma using a SCIEX 6500+ triple QUAD LC-MS system equipped with an ExionLC UHPLC unit. GT-14 (m/z 265.2 → 134.1) and griseofulvin (Internal Standard, IS) (m/z 353.1 → 285.1) were detected in a positive mode by electrospray ionization (ESI) using multiple reaction monitoring (MRM). The assay was linear in the concentration range of 0.78-1000 ng/mL in rat plasma. Both accuracy and precision values were within the acceptance criteria of ±15 %, as established by FDA guidance. The matrix effect was negligible from plasma, with signal percentages of 98.5-106.9 %. The mean recovery was 104.5 %, indicating complete extraction of GT-14 from plasma. GT-14 was found to be stable under different experimental conditions. The validated method was successfully applied to evaluate plasma protein binding and in vivo pharmacokinetics of GT-14 in rats., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF