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308 results on '"Grillo, Giorgio"'

7. Optimization of ultrasound pretreatment and enzymatic hydrolysis of wheat straw: From lab to semi-industrial scale

Author

Calcio Gaudino, Emanuela, Grillo, Giorgio, Tabasso, Silvia, Stevanato, Livio, Cravotto, Giancarlo, Marjamaa, Kaisa, Pihlajaniemi, Ville, Koivula, Anu, Aro, Nina, Uusitalo, Jaana, Ropponen, Jarmo, Kuutti, Lauri, Kivinen, Pauliina, Kanerva, Heimo, Arshanitsa, Alexander, Jashina, Lilija, Jurkjane, Vilhelmine, Andersone, Anna, Dreyer, Thomas, and Schories, Gerhard

Published
2022
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8. Industrial Production of Bioactive Nutrient-Enhanced Extra Virgin Olive Oil under Continuous-Flow Ultrasound and Pulsed Electric Field Treatment.

Author

Boffa, Luisa, Calcio Gaudino, Emanuela, Grillo, Giorgio, Binello, Arianna, Capaldi, Giorgio, Rego, Duarte, Pereira, Marcos, and Cravotto, Giancarlo

Subjects
OLIVE oil mills, OLIVE oil, MANUFACTURING processes, MEDITERRANEAN diet, ELECTRIC fields
Abstract

Extra virgin olive oil (EVOO) is a cornerstone of the Mediterranean diet. Many studies have highlighted its crucial preventive role against cardiovascular disease, neurodegenerative disorders, metabolic syndrome and cancer, with these effects being due to the synergistic anti-inflammatory and antioxidant activities of minor components, such as polyphenols and tocols. The aim of the present study is to implement new technologies for olive oil mills and develop an efficient large-sized industrial process for the continuous extraction of healthier EVOOs that are enriched with these bioactive compounds. Non-thermal technologies, namely ultrasound (US) and pulsed electric field (PEF), have been tested, separately and in combination, to eliminate the need for traditional malaxation. There is extensive literature to support the efficacy of ultrasound-assisted extraction (UAE) and PEF treatments in EVOO production. A newly designed US device and a PEF industrial chamber have been combined into a single, integrated continuous-flow setup, the performance of which in the extraction of EVOO from green Coratina olives has been evaluated herein. Extraction yields, physico-chemical and organoleptic characteristics, and polyphenol and tocol contents were monitored throughout the trials, and the last three were measured at accelerated aging times (AAT) of 15 and 30 days. The US and combined US-PEF processes not only increased daily oil production (ton/day, by nearly 45%), but also eliminated the need for kneading during malaxation, resulting in significant energy savings (approximately 35%). In addition, these innovations enriched the resulting EVOO with nutritionally relevant minor components (8–12% polyphenols, 3–5% tocols), thereby elevating its quality and market value, as well as overall stability. The introduction of continuous-flow US and PEF technologies is a remarkable innovation for the EVOO industry, as they offer benefits to both producers and consumers. The EVOO resulting from non-thermal continuous-flow production meets the growing demand for healthier, nutrient-enriched products. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Batch and Flow Green Microwave‐Assisted Catalytic Conversion Of Levulinic Acid to Pyrrolidones.

Author

Calcio Gaudino, Emanuela, Manzoli, Maela, Testa, Maria Luisa, La Parola, Valeria, Grillo, Giorgio, Cravotto, Giancarlo, Acciardo, Elisa, and Tabasso, Silvia

Subjects
MESOPOROUS silica, CATALYST supports, METHYL ether, ORGANIC solvents, CONTINUOUS processing, BIMETALLIC catalysts
Abstract

This paper reports a new sustainable protocol for the microwave‐assisted catalytic conversion of levulinic acid into N‐substituted pyrrolidones over tailor‐made mono (Pd, Au) or bimetallic (PdAu) catalysts supported on either highly mesoporous silica (HMS) or titania‐doped HMS, exploiting the advantages of dielectric heating. MW‐assisted reductive aminations of levulinic acid with several amines were first optimized in batch mode under hydrogen pressure (5 bar) in solvent‐free conditions. Good‐to‐excellent yields were recorded at 150 °C in 90 min over the PdTiHMS and PdAuTiHMS, that proved recyclable and almost completely stable after six reaction cycles. Aiming to scale‐up this protocol, a MW‐assisted flow reactor was used in combination with different green solvents. Cyclopentyl methyl ether (CPME) provided a 99 % yield of N‐(4‐methoxyphenyl) pyrrolidin‐2‐one at 150 °C over PdTiHMS. The described MW‐assisted flow synthesis proves to be a safe procedure suitable for further industrial applications, while averting the use of toxic organic solvents. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Semi‐industrial ultrasound‐assisted extraction of grape‐seed proteins.

Author

Chaji, Salah, Capaldi, Giorgio, Gallina, Lorenzo, Grillo, Giorgio, Boffa, Luisa, and Cravotto, Giancarlo

Subjects
GRAPE seeds, PROTEINS, GALLIC acid, INDUSTRIAL wastes, CHEMICAL industry, CIRCULAR RNA
Abstract

BACKGROUND: To manage industrial waste in accordance with the circular bioeconomy concept it is sometimes necessary to handle grape seeds, an abundant by‐product of the wine‐making process. This study presents a process based on ultrasound technology for the extraction of grape‐seed proteins, due to their nutritional and techno‐functional properties. The protein content of extracts obtained under silent and lab‐scale conditions was compared with that obtained under semi‐industrial ultrasound conditions, and the chemical composition (carbohydrates, total phenols, and lipids) and the elemental profiles of the final, up‐scaled downstream extracts were characterized. RESULTS: This work found that the maximum amount of protein in the final product was 378.31 g.kg−1 of the extract. Chemical characterization revealed that each 1 kg of extract had an average content of 326.19 g gallic acid equivalent as total phenols, 162.57 g glucose equivalent as carbohydrates, and 382.76 g of lipophilic compounds. Furthermore, when the extract was checked for hazardous elements, none were found in levels that could be considered a risk for human health. CONCLUSION: The proposed semi‐industrial strategy has the potential to contribute greatly to the valorization of grape seeds through the preparation of a protein‐rich extract that can be used as an alternative to synthetic wine stabilizers and for the development of novel food and nutraceutical products. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Published
2024
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22. Managing NGS Differential Expression Uncertainty with Fuzzy Sets

Author

Consiglio, Arianna, Mencar, Corrado, Grillo, Giorgio, Liuni, Sabino, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Weikum, Gerhard, Series editor, Angelini, Claudia, editor, Rancoita, Paola MV, editor, and Rovetta, Stefano, editor

Published
2016
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39. Transmembrane Protein TMEM230, a Target of Glioblastoma Therapy

Author

Cocola, Cinzia, primary, Magnaghi, Valerio, additional, Abeni, Edoardo, additional, Pelucchi, Paride, additional, Martino, Valentina, additional, Vilardo, Laura, additional, Piscitelli, Eleonora, additional, Consiglio, Arianna, additional, Grillo, Giorgio, additional, Mosca, Ettore, additional, Gualtierotti, Roberta, additional, Mazzaccaro, Daniela, additional, La Sala, Gina, additional, Di Pietro, Chiara, additional, Palizban, Mira, additional, Liuni, Sabino, additional, DePedro, Giuseppina, additional, Morara, Stefano, additional, Nano, Giovanni, additional, Kehler, James, additional, Greve, Burkhard, additional, Noghero, Alessio, additional, Marazziti, Daniela, additional, Bussolino, Federico, additional, Bellipanni, Gianfranco, additional, D’Agnano, Igea, additional, Götte, Martin, additional, Zucchi, Ileana, additional, and Reinbold, Rolland, additional

Published
2021
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43. Deep eutectic solvents and non-conventional technologies for blueberry peels extraction: kinetics, anthocyanins stability and antiproliferative activity

Author

Gunjević, Veronika, Grillo, Giorgio, Radošević, Kristina, Radojčić Redovniković, Ivana, Cravotto, Giancarlo, Vaccaro, Luigi, Capacchione, Carmine, Grillo, Giorgio, Licini, Giulia, Salvini, Antonella, Samori, Chiara, Massi, Alessandro, and Zaccheria, Federica

Subjects
deep eutectic solvents, anthocyanins, antiproliferative activity, shelf-life, extraction kinetics
Abstract

In recent years, food industry by-product’s high added value compounds recovery using non- conventional technologies is gaining increasing attention, since it can reduce environmental impact and ensure economic benefits.1 In the light of the aforesaid, two emerging extraction methods for anthocyanins recovery from blueberry processing residues were studied to obtain a novel antitumor agent. Firstly, five natural deep eutectic solvents (NADES) have been tested for anthocyanin extraction and the eutectic systems shelf-life was monitored over 9 months. The most suitable NADES was implemented in microwave- (MAE) and ultrasound-assisted extractions (UAE). Extraction kinetics was described using Peleg’s model. Both technologies enabled the performance and gave a result superior to that of conventional extraction performed using hydroalcoholic solution. MAE and UAE yielded up to 25.83 and 21.18 mg/gmatrix of total anthocyanin content, respectively, after 15 and 30 min. Finally, extracts antiproliferative activity was determined by an MTS assay on human tumour HeLa cells against human skin cells. Non-conventional extracts exhibited strong antiproliferative activity, much greater than that of their conventional analogue. Cell-death type was evaluated by flow cytometry, and apoptosis was found to be the primary cause of tumour cell death.

Published
2020

45. CeO2Frustrated Lewis Pairs Improving CO2and CH3OH Conversion to Monomethylcarbonate

Author

Salusso, Davide, Grillo, Giorgio, Manzoli, Maela, Signorile, Matteo, Zafeiratos, Spyridon, Barreau, Mathias, Damin, Alessandro, Crocellà, Valentina, Cravotto, Giancarlo, and Bordiga, Silvia

Abstract

Frustrated Lewis pairs (FLPs), discovered in the last few decades for homogeneous catalysts and in the last few years also for heterogeneous catalysts, are stimulating the scientific community’s interest for their potential in small-molecule activation. Nevertheless, how an FLP activates stable molecules such as CO2is still undefined. Through a careful spectroscopic study, we here report the formation of FLPs over a highly defective CeO2sample prepared by microwave-assisted synthesis. Carbon dioxide activation over FLP is shown to occur through a bidentate carbonate bridging the FLP and implying a Ce3+-to-CO2charge transfer, thus enhancing its activation. Carbon dioxide reaction with methanol to form monomethylcarbonate is here employed to demonstrate active roles of FLP and, eventually, to propose a reaction mechanism clarifying the role of Ce3+and oxygen vacancies.

Published
2023
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47. Lignin – Derived antioxidants as value-added products obtained under cavitation treatments of the wheat straw processing for sugar production

Author

Lauberte, Liga, primary, Telysheva, Galina, additional, Cravotto, Giancarlo, additional, Andersone, Anna, additional, Janceva, Sarmite, additional, Dizhbite, Tatiana, additional, Arshanitsa, Alexandr, additional, Jurkjane, Vilhelmine, additional, Vevere, Laima, additional, Grillo, Giorgio, additional, Gaudino, Emanuela Calcio, additional, and Tabasso, Silvia, additional

Published
2021
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