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132 results on '"Grignani, Elena"'

1. Phase I Clinical Trial on Pleural Mesothelioma Using Neoadjuvant Local Administration of Paclitaxel-Loaded Mesenchymal Stromal Cells (PACLIMES Trial): Study Rationale and Design.

Author

Stella, Giulia Maria, Lisini, Daniela, Pedrazzoli, Paolo, Galli, Giulia, Bortolotto, Chandra, Melloni, Giulio, D'Ambrosio, Gioacchino, Klersy, Catherine, Grosso, Amelia, Paino, Francesca, Tomaselli, Stefano, Saracino, Laura, Alessandri, Giulio, Pessina, Augusto, Grignani, Elena, Rosti, Vittorio, Corsico, Angelo Guido, Comoli, Patrizia, and Agustoni, Francesco

Subjects
MESENCHYMAL stem cells, CLINICAL trials, PLEURAL tumors, TREATMENT effectiveness, COMBINED modality therapy, MESOTHELIOMA, PACLITAXEL
Abstract

Simple Summary: The phase I monocentric PACLIMES trial evaluates the effects of local administration of an innovative tool composed of mesenchymal stromal cells loaded with paclitaxel directly injected in the pleural space in mesothelioma patients in a neoadjuvant setting. The trial explores the safety and toxicity of the drug product, but also an exploratory objective aimed at evaluating its antiproliferative capacity. The results of this study will validate a promising therapeutic option for an unmet clinical need such as pleural mesothelioma and open the way to an efficient management of malignant pleural effusion in case of advanced cancers from different sites of primary origin. Background and rationale. Pleural mesothelioma (PM) is a rare and aggressive neoplasm that originates from the pleural mesothelium and whose onset is mainly linked to exposure to asbestos, which cannot be attacked with truly effective therapies with consequent poor prognosis. The rationale of this study is based on the use of mesenchymal stromal cells (MSCs) as a vehicle for chemotherapy drugs to be injected directly into the pathological site, such as the pleural cavity. Study design. The study involves the use of a conventional chemotherapeutic drug, Paclitaxel (PTX), which is widely used in the treatment of different types of solid tumors, including PM, although some limitations are related to pharmacokinetic aspects. The use of PTX-loaded MSCs to treat PM should provide several potential advantages over the systemically administered drug as reduced toxicity and increased concentration of active drug in the tumor-surrounding context. The PACLIMES trial explores the safety and toxicity of the local administration of Paclimes in chemonaive patients, candidates for pleurectomy. The secondary objective is to find the effective Paclimes dose for subsequent phase II studies and to observe and record the antitumor activity. Future direction. The experimental pre-clinical background and rationale are discussed as well. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Combined Ferritin Nanocarriers with ICG for Effective Phototherapy Against Breast Cancer

Author

Sitia,Leopoldo, Saccomandi,Paola, Bianchi,Leonardo, Sevieri,Marta, Sottani,Cristina, Allevi,Raffaele, Grignani,Elena, Mazzucchelli,Serena, Corsi,Fabio, Sitia,Leopoldo, Saccomandi,Paola, Bianchi,Leonardo, Sevieri,Marta, Sottani,Cristina, Allevi,Raffaele, Grignani,Elena, Mazzucchelli,Serena, and Corsi,Fabio

Abstract

Leopoldo Sitia,1,* Paola Saccomandi,2,* Leonardo Bianchi,2 Marta Sevieri,1 Cristina Sottani,3 Raffaele Allevi,1 Elena Grignani,3 Serena Mazzucchelli,1 Fabio Corsi1,4 1Department of Biomedical and Clinical Sciences, Università degli studi di Milano, Milan, Italy; 2Department of Mechanical Engineering, Politecnico di Milano, Milan, Italy; 3Environmental Research Center, Istituti Clinici Scientifici Maugeri IRCCS, Pavia, Italy; 4Istituti Clinici Scientifici Maugeri IRCCS, Pavia, Italy*These authors contributed equally to this workCorrespondence: Serena Mazzucchelli, Department of Biomedical and Clinical Sciences, Università degli studi di Milano, via G.B. Grassi 74, Milan, 20157, Italy, Email serena.mazzucchelli@unimi.it Fabio Corsi, Istituti Clinici Scientifici Maugeri IRCCS, via S. Maugeri 10, Pavia, 27100, Italy, Email fabio.corsi@unimi.itIntroduction: Photodynamic Therapy (PDT) is a promising, minimally invasive treatment for cancer with high immunostimulatory potential, no reported drug resistance, and reduced side effects. Indocyanine Green (ICG) has been used as a photosensitizer (PS) for PDT, although its poor stability and low tumor-target specificity strongly limit its efficacy. To overcome these limitations, ICG can be formulated as a tumor-targeting nanoparticle (NP).Methods: We nanoformulated ICG into recombinant heavy-ferritin nanocages (HFn-ICG). HFn has a specific interaction with transferrin receptor 1 (TfR1), which is overexpressed in most tumors, thus increasing HFn tumor tropism. First, we tested the properties of HFn-ICG as a PS upon irradiation with a continuous-wave diode laser. Then, we evaluated PDT efficacy in two breast cancer (BC) cell lines with different TfR1 expression levels. Finally, we measured the levels of intracellular endogenous heavy ferritin (H-Fn) after PDT treatment. In fact, it is known that cells undergoing ROS-induced autophagy, as in PDT, tend to increase their ferritin levels as a defence mechanis

Published
2024

6. Deciphering the Role of H-Ferritin Nanocages in Improving Tumor-Targeted Delivery of Indocyanine Green: Combined Analysis of Murine Tissue Homogenates with UHPLC–MS/MS and Fluorescence

Author

Sevieri, Marta, primary, Sottani, Cristina, additional, Chesi, Arianna, additional, Bonizzi, Arianna, additional, Sitia, Leopoldo, additional, Robustelli Della Cuna, Francesco Saverio, additional, Grignani, Elena, additional, Corsi, Fabio, additional, and Mazzucchelli, Serena, additional

Published
2023
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12. Occupational Exposure Assessment to Antineoplastic Drugs in Nine Italian Hospital Centers over a 5-Year Survey Program

Author

Sottani, Cristina, primary, Grignani, Elena, additional, Cornacchia, Marco, additional, Negri, Sara, additional, Cuna, Francesco Saverio Robustelli della, additional, Cottica, Danilo, additional, Bruzzese, Dario, additional, Severi, Paolo, additional, Strocchi, Daniele, additional, Verna, Giovanni, additional, Leso, Veruscka, additional, and Iavicoli, Ivo, additional

Published
2022
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15. Development and Validation of a Bioanalytical UHPLC-MS/MS Method Applied to Murine Liver Tissue for the Determination of Indocyanine Green Loaded in H-Ferritin Nanoparticles

Author

Sottani, Cristina, primary, Grignani, Elena, additional, Cottica, Danilo, additional, Mazzucchelli, Serena, additional, Sevieri, Marta, additional, Chesi, Arianna, additional, Corsi, Fabio, additional, Galfrè, Sarah, additional, Robustelli della Cuna, Francesco Saverio, additional, and Calleri, Enrica, additional

Published
2022
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16. Particle measurements of metal additive manufacturing to assess working occupational exposures: a comparative analysis of selective laser melting, laser metal deposition and hybrid laser metal deposition

Author

ODDONE, Enrico, primary, PERNETTI, Roberta, additional, FIORENTINO, Maria Lorena, additional, GRIGNANI, Elena, additional, TAMBORINI, Daniele, additional, ALAIMO, Gianluca, additional, AURICCHIO, Ferdinando, additional, PREVITALI, Barbara, additional, and IMBRIANI, Marcello, additional

Published
2021
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17. Validation of cleaning procedures used in an Italian Hospital Pharmacy for antineoplastic drug decontamination: a new tool for industrial hygiene

Author

Negri, Sara, Oddone, Enrico, Morandi, Francesco, Sottani, Cristina, Gardinali, Francesco, Lillo, Annalisa, Pastoris, Ornella, Dacrema, Valerio, Losurdo, Anna, Grignani, Elena, Cottica, Danilo, and Imbriani, Marcello

Subjects
surface decontamination, wipe test, Antineoplastic Agents, occupational exposure, hospital pharmacy, Italy, Tandem Mass Spectrometry, Equipment Contamination, Original Article, Pharmacy Service, Hospital, Antineoplastic drugs, cleaning procedure, Decontamination, Occupational Health, Chromatography, Liquid
Abstract

Background: Current Italian regulations and procedures for surface decontamination of antineoplastic drugs (ADs) are not clear. Therefore, most hospital pharmacies follow internal procedures as an interpretation of the recommended handling guidelines. Objectives: Our study compared 7 different cleaning procedures after controlled contamination of the work surface of a biological safety cabinet workbench in an Italian hospital oncology pharmacy (HOP) to determine which of them is more efficient and practical. Moreover, in order to approximate operative routine and improve risk awareness, cleaning procedures were carried out by the personnel that usually operate in the HOP. Methods: Measured quantities, i.e. a drop (100 μL) of 5-FluoroUracil, IPhosfamide, CycloPhosphamide and Gemcitabine, were deposited on the work surface within precisely delimited areas. Following the wipe-test analysis using UPLC-MS/MS, the cleaning efficacy was calculated based on the ratio of the residual concentration of the AD, after the cleaning procedure, to the concentration of each AD before the procedure. Results: Tested cleaning procedures were: 1) Hypo-Chlor®, hot water and Farmecol70®; 2) Hypo-Chlor® and hot water; 3) Farmecol70®; 4) Surfa’Safe SH® and hot water; 5) Amuchina® 10%, hot water and Farmecol70®; 6) Incidin® Oxyfoam and hot water; 7) liquid Marseille soap, hot water and Farmecol70®. Within the studied HOP, the Marseille soap was evaluated to be the optimal choice due to its efficacy, low cost, and the very short contact time needed before rinsing. Discussion: The application of the protocol for procedure validation suggested here could be used in every HOP as a reliable industrial hygiene tool to demonstrate the validity of the chosen cleaning procedure.

Published
2019

21. Selective Targeting of Cancer-Associated Fibroblasts by Engineered H-Ferritin Nanocages Loaded with Navitoclax

Author

Sitia, Leopoldo, Bonizzi, Arianna, Mazzucchelli, Serena, Negri, Sara, Sottani, Cristina, Grignani, Elena, Rizzuto, Maria Antonietta, Prosperi, Davide, Sorrentino, Luca, Morasso, Carlo, Allevi, Raffaele, Sevieri, Marta, Silva, Filippo, Truffi, Marta, Corsi, Fabio, Sitia, L, Bonizzi, A, Mazzucchelli, S, Negri, S, Sottani, C, Grignani, E, Rizzuto, M, Prosperi, D, Sorrentino, L, Morasso, C, Allevi, R, Sevieri, M, Silva, F, Truffi, M, and Corsi, F

Subjects
fibroblast activation protein, Sulfonamides, congenital, hereditary, and neonatal diseases and abnormalities, Aniline Compounds, Microscopy, Confocal, Tissue Engineering, Cancer-associated fibroblast, navitoclax, H-ferritin, Membrane Proteins, Antineoplastic Agents, Article, digestive system diseases, Mice, lcsh:Biology (General), Apoferritins, Animals, Humans, Nanoparticles, Female, targeted nanoparticles, neoplasms, cancer-associated fibroblasts, lcsh:QH301-705.5, Targeted nanoparticle
Abstract

Cancer-associated fibroblasts (CAFs) are key actors in regulating cancer progression. They promote tumor growth, metastasis formation, and induce drug resistance. For these reasons, they are emerging as potential therapeutic targets. Here, with the aim of developing CAF-targeted drug delivery agents, we functionalized H-ferritin (HFn) nanocages with fibroblast activation protein (FAP) antibody fragments. Functionalized nanocages (HFn-FAP) have significantly higher binding with FAP+ CAFs than with FAP− cancer cells. We loaded HFn-FAP with navitoclax (Nav), an experimental Bcl-2 inhibitor pro-apoptotic drug, whose clinical development is limited by its strong hydrophobicity and toxicity. We showed that Nav is efficiently loaded into HFn (HNav), maintaining its mechanism of action. Incubating Nav-loaded functionalized nanocages (HNav-FAP) with FAP+ cells, we found significantly higher cytotoxicity as compared to non-functionalized HNav. This was correlated with a significantly higher drug release only in FAP+ cells, confirming the specific targeting ability of functionalized HFn. Finally, we showed that HFn-FAP is able to reach the tumor and to target CAFs in a mouse syngeneic model of triple negative breast cancer after intravenous administration. Our data show that HNav-FAP could be a promising tool to enhance specific drug delivery into CAFs, thus opening new therapeutic possibilities focused on tumor microenvironment.

Published
2021

22. Selective Targeting of Cancer-Associated Fibroblasts by Engineered H-Ferritin Nanocages Loaded with Navitoclax

Author

Sitia, L, Bonizzi, A, Mazzucchelli, S, Negri, S, Sottani, C, Grignani, E, Rizzuto, M, Prosperi, D, Sorrentino, L, Morasso, C, Allevi, R, Sevieri, M, Silva, F, Truffi, M, Corsi, F, Sitia, Leopoldo, Bonizzi, Arianna, Mazzucchelli, Serena, Negri, Sara, Sottani, Cristina, Grignani, Elena, Rizzuto, Maria Antonietta, Prosperi, Davide, Sorrentino, Luca, Morasso, Carlo, Allevi, Raffaele, Sevieri, Marta, Silva, Filippo, Truffi, Marta, Corsi, Fabio, Sitia, L, Bonizzi, A, Mazzucchelli, S, Negri, S, Sottani, C, Grignani, E, Rizzuto, M, Prosperi, D, Sorrentino, L, Morasso, C, Allevi, R, Sevieri, M, Silva, F, Truffi, M, Corsi, F, Sitia, Leopoldo, Bonizzi, Arianna, Mazzucchelli, Serena, Negri, Sara, Sottani, Cristina, Grignani, Elena, Rizzuto, Maria Antonietta, Prosperi, Davide, Sorrentino, Luca, Morasso, Carlo, Allevi, Raffaele, Sevieri, Marta, Silva, Filippo, Truffi, Marta, and Corsi, Fabio

Abstract

Cancer-associated fibroblasts (CAFs) are key actors in regulating cancer progression. They promote tumor growth, metastasis formation, and induce drug resistance. For these reasons, they are emerging as potential therapeutic targets. Here, with the aim of developing CAF-targeted drug delivery agents, we functionalized H-ferritin (HFn) nanocages with fibroblast activation protein (FAP) antibody fragments. Functionalized nanocages (HFn-FAP) have significantly higher binding with FAP+ CAFs than with FAP cancer cells. We loaded HFn-FAP with navitoclax (Nav), an experimental Bcl-2 inhibitor pro-apoptotic drug, whose clinical development is limited by its strong hydrophobicity and toxicity. We showed that Nav is efficiently loaded into HFn (HNav), maintaining its mechanism of action. Incubating Nav-loaded functionalized nanocages (HNav-FAP) with FAP+ cells, we found significantly higher cytotoxicity as compared to non-functionalized HNav. This was correlated with a significantly higher drug release only in FAP+ cells, confirming the specific targeting ability of functionalized HFn. Finally, we showed that HFn-FAP is able to reach the tumor and to target CAFs in a mouse syngeneic model of triple negative breast cancer after intravenous administration. Our data show that HNav-FAP could be a promising tool to enhance specific drug delivery into CAFs, thus opening new therapeutic possibilities focused on tumor microenvironment.

Published
2021

23. Particle measurements of metal additive manufacturing to assess working occupational exposures: a comparative analysis of selective laser melting, laser metal deposition and hybrid laser metal deposition.

Author

ODDONE, Enrico, PERNETTI, Roberta, FIORENTINO, Maria Lorena, GRIGNANI, Elena, TAMBORINI, Daniele, ALAIMO, Gianluca, AURICCHIO, Ferdinando, PREVITALI, Barbara, and IMBRIANI, Marcello

Abstract

This paper presents the results of a measurement campaign for assessing the release of particles and the potential exposure of workers in metal additive manufacturing. The monitoring deals with three environments, i.e., two academic laboratories and one production site, while printing different metallic alloys for chemical composition and size. The monitored devices implement different metal 3D printing processes, named Selective Laser Melting, Laser Metal Deposition and Hybrid Laser Metal Deposition, providing a wide overview of the current laserbased Additive Manufacturing technologies. Despite showing the generation of metal powders during the printing processes, the usual measurements based on gravimetric analysis did not highlight concentrations higher than the international exposure limits for the selected metals (i.e., chromium, cobalt, iron, nickel, and copper). Additional data, collected through a cascade impactor and particle counter coupled with the achievements from previous measurements reported in literature, indicate that during the printing operations, fine and ultrafine metal particles might be generated. Finally, the authors introduced a preliminary characterisation of the particles released during the different phases of the investigated AM processes (powder charging, printing, part cleaning and support removal), highlighting how the different operations may affect the particle size and concentration. [ABSTRACT FROM AUTHOR]

Published
2022
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24. Biomarkers of Low-Level Environmental Exposure to Benzene and Oxidative DNA Damage in Primary School Children in Sardinia, Italy

Author

Pilia, Ilaria, primary, Campagna, Marcello, additional, Marcias, Gabriele, additional, Fabbri, Daniele, additional, Meloni, Federico, additional, Spatari, Giovanna, additional, Cottica, Danilo, additional, Cocheo, Claudio, additional, Grignani, Elena, additional, De-Giorgio, Fabio, additional, Cocco, Pierluigi, additional, and d’Aloja, Ernesto, additional

Published
2021
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26. Accurate low-dose exposure assessment of benzene and monoaromatic compounds by diffusive sampling: Sampling and analytical method validation according to ISO 23320 for radiello® samplers packed with graphitised charcoal and suitable for thermal desorption

Author

Zaratin, Laura, Boaretto, Caterina, Schianca, Riccardo Carnevale, Hinkal, George, Grignani, Elena, and Cottica, Danilo

Abstract

•Benzene exposure by inhalation at the workplace has decreased in recent years.•High quality data are needed to study health effects of benzene low dose exposure.•A diffusive method has been validated for benzene monitoring at low concentration.•Sampling and analysis method validated according to ISO 23320 standard.•Radiello® diffusive samplers with graphitized charcoal fit for benzene monitoring.

Published
2024
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31. Everolimus Nanoformulation in Biological Nanoparticles Increases Drug Responsiveness in Resistant and Low-Responsive Breast Cancer Cell Lines

Author

Bonizzi, Arianna, Truffi, Marta, Sevieri, Marta, Allevi, Raffaele, Sitia, Leopoldo, Ottria, Roberta, Sorrentino, Luca, Sottani, Cristina, Negri, Sara, Grignani, Elena, Mazzucchelli, Serena, and Corsi, Fabio

Subjects
lcsh:Pharmacy and materia medica, animal structures, breast cancer, H-ferritin, embryonic structures, fungi, lcsh:RS1-441, nanoparticles, Everolimus, Article
Abstract

Everolimus (Eve) is an FDA approved drug that inhibits mammalian target of rapamycin (mTOR). It is employed in breast cancer treatment even if its responsiveness is controversial. In an attempt to increase Eve effectiveness, we have developed a novel Eve nanoformulation exploiting H-ferritin nanocages (HEve) to improve its subcellular delivery. We took advantage of the natural tumor targeting of H-Ferritin, which is mediated by the transferrin receptor-1 (TfR1). Breast cancer cells overexpressing TfR-1 were successfully recognized by H-Ferritin, displaying quick nanocage internalization. HEve has been tested and compared to Eve for in vitro efficacy in sensitive and resistant breast cancer cells. Nanoformulated Eve induced remarkable antiproliferative activity in vitro, making even resistant cell lines sensitive to Eve. Moreover, the antiproliferative activity of HEve is fully in accordance with cytotoxicity observed by cell death assay. Furthermore, the significant increase in anticancer efficacy displayed in HEve-treated samples is due to the improved drug accumulation, as demonstrated by UHPLC-MS/MS quantifications. Our findings suggest that optimizing Eve subcellular delivery, thanks to nanoformulation, determines its improved antitumor activity in a panel of Eve-sensitive or resistant breast cancer cell lines.

Published
2019

34. Chemical Composition of the Essential Oils From Leaves and Flowers of Passiflora sexocellata and Passiflora trifasciata.

Author

Robustelli della Cuna, Francesco Saverio, Giovannini, Annalisa, Braglia, Luca, Sottani, Cristina, Grignani, Elena, and Preda, Stefania

Subjects
THERAPEUTIC use of essential oils, PASSIFLORA, PLANT extracts, HYDROCARBONS, ALDEHYDES
Abstract

The chemical composition of the essential oils of Passiflora sexocellata and Passiflora trifasciata (Passifloraceae, subgenus Decaloba) were studied for the first time. Essential oils were obtained by steam distillation of fresh leaves and flowers. The chemical composition was assessed by using GC/FID and GC/MS. For P. sexocellata leaves, the optimized analytical procedure allowed the identification of 33 compounds (75% of the total oil composition) and 29 (74% of the total oil composition) in flowers. Regarding P. trifasciata, 35 compounds (76% of the total oil composition) were detected in leaves and 32 (71% of the total oil composition) in flowers. Terpenes and mono unsaturated hydrocarbons were quantified as major constituents of the volatile fraction in flowers (17.0 to 52.6%) and (13.7 to 20.0%). Organic acids were detected in both leaves and flowers with a percentage ranging from 3.3% to 32.0%. Aldehydes were also detected in leaves (12.6 to 41.4%) and in flowers (1.4 to 5.1%). The GC/MS analyzes allowed alcohols to be detected in leaves (20.6 to 42.9%) and in flowers (8.2 to 18.1%). These compounds represent the most important feature of the large Passiflora family. Moreover, a critical role in the coevolved mechanisms of pollinators' interaction has been investigated. [ABSTRACT FROM AUTHOR]

Published
2021
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35. A new, sensitive and versatile assay for quantitative determination of α-fluoro-β-alanine (AFBA) in human urine by using the reversed-phase ultrahigh performance-tandem mass spectrometry (rp-UHPLC–MS/MS) system

Author

Sottani, Cristina, primary, Grignani, Elena, additional, Zaratin, Laura, additional, Santorelli, Donatella, additional, Studioso, Emanuele, additional, Lonati, Davide, additional, Locatelli, Carlo A., additional, Pastoris, Ornella, additional, Negri, Sara, additional, and Cottica, Danilo, additional

Published
2018
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38. Biallelic mutations in SORDcause a common and potentially treatable hereditary neuropathy with implications for diabetes

Author

Cortese, Andrea, Zhu, Yi, Rebelo, Adriana P., Negri, Sara, Courel, Steve, Abreu, Lisa, Bacon, Chelsea J., Bai, Yunhong, Bis-Brewer, Dana M., Bugiardini, Enrico, Buglo, Elena, Danzi, Matt C., Feely, Shawna M. E., Athanasiou-Fragkouli, Alkyoni, Haridy, Nourelhoda A., Isasi, Rosario, Khan, Alaa, Laurà, Matilde, Magri, Stefania, Pipis, Menelaos, Pisciotta, Chiara, Powell, Eric, Rossor, Alexander M., Saveri, Paola, Sowden, Janet E., Tozza, Stefano, Vandrovcova, Jana, Dallman, Julia, Grignani, Elena, Marchioni, Enrico, Scherer, Steven S., Tang, Beisha, Lin, Zhiqiang, Al-Ajmi, Abdullah, Schüle, Rebecca, Synofzik, Matthis, Maisonobe, Thierry, Stojkovic, Tanya, Auer-Grumbach, Michaela, Abdelhamed, Mohamed A., Hamed, Sherifa A., Zhang, Ruxu, Manganelli, Fiore, Santoro, Lucio, Taroni, Franco, Pareyson, Davide, Houlden, Henry, Herrmann, David N., Reilly, Mary M., Shy, Michael E., Zhai, R. Grace, and Zuchner, Stephan

Abstract

Here we report biallelic mutations in the sorbitol dehydrogenase gene (SORD) as the most frequent recessive form of hereditary neuropathy. We identified 45 individuals from 38 families across multiple ancestries carrying the nonsense c.757delG (p.Ala253GlnfsTer27) variant in SORD, in either a homozygous or compound heterozygous state. SORD is an enzyme that converts sorbitol into fructose in the two-step polyol pathway previously implicated in diabetic neuropathy. In patient-derived fibroblasts, we found a complete loss of SORD protein and increased intracellular sorbitol. Furthermore, the serum fasting sorbitol levels in patients were dramatically increased. In Drosophila, loss of SORDorthologs caused synaptic degeneration and progressive motor impairment. Reducing the polyol influx by treatment with aldose reductase inhibitors normalized intracellular sorbitol levels in patient-derived fibroblasts and in Drosophila, and also dramatically ameliorated motor and eye phenotypes. Together, these findings establish a novel and potentially treatable cause of neuropathy and may contribute to a better understanding of the pathophysiology of diabetes.

Published
2020
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41. Il rischio chimico e cancerogeno nell'industria siderurgica.

Author

Cottica, Danilo, Grignani, Elena, De Palma, Giuseppe, and Lovreglio, Piero

Abstract

Copyright of Giornale Italiano di Medicina del Lavoro ed Ergonomia is the property of Giornale Italiano di Medicina del Lavoro ed Ergonomia Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2018

43. Rischi da lavoro nel settore della ristorazione.

Author

Cottica, Danilo and Grignani, Elena

Subjects
COOKING, THERMAL comfort, CONVENIENCE foods, HIGH temperatures, PART-time employees
Abstract

Copyright of Giornale Italiano di Medicina del Lavoro ed Ergonomia is the property of Giornale Italiano di Medicina del Lavoro ed Ergonomia Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2014

44. Evoluzioni della tecnologia e delle esposizioni professionali nell'industria petrolchimica e nella raffinazione del petrolio.

Author

Cottica, Danilo and Grignani, Elena

Subjects
*OCCUPATIONAL hazards, *PETROLEUM refining, *ORGANIC compounds, *COMMERCIAL products, *AROMATIC compounds
Abstract

The article discusses a study related to the exposure to occupational hazards in petrochemical industry and petroleum refining. Topics discussed include petrochemical uses raw materials including oil fractions which are obtained by distillation in refinery, or natural gas, oil refining uses oil to obtain mixtures of hydrocarbon compounds, and chemical agents are involved manufacturing processes of petroleum products including benzene, toluene, and xylenes.

Published
2013

45. La Valutazione del Rischio da Agenti Chimici.

Author

Cottica, Danilo, Grignani, Elena, and Bartolucci, Giovanni Battista

Subjects
*RISK assessment, *CHEMICALS, *INDUSTRIAL hygienists, *OCCUPATIONAL physicians, *THRESHOLD limit values (Industrial toxicology)
Abstract

The article focuses on the application of risk assessment of chemical agents by industrial hygienists and occupational physicians based on exposure evaluation. It introduces changes and instruments in carrying out the occupational exposure scenario that industrial hygienists and occupational physicians should evaluate. It looks at the relevance of control measures and evaluation of exposure scenario and report the flowchart of risk assessment and management.

Published
2011

47. Chemical Composition of the Essential Oils From Leaves and Flowers of Passiflora sexocellataand Passiflora trifasciata

Author

della Cuna, Francesco Saverio Robustelli, Giovannini, Annalisa, Braglia, Luca, Sottani, Cristina, Grignani, Elena, and Preda, Stefania

Abstract

The chemical composition of the essential oils of Passiflora sexocellataand Passiflora trifasciata(Passifloraceae, subgenus Decaloba) were studied for the first time. Essential oils were obtained by steam distillation of fresh leaves and flowers. The chemical composition was assessed by using GC/FID and GC/MS. For P. sexocellataleaves, the optimized analytical procedure allowed the identification of 33 compounds (75% of the total oil composition) and 29 (74% of the total oil composition) in flowers. Regarding P. trifasciata,35 compounds (76% of the total oil composition) were detected in leaves and 32 (71% of the total oil composition) in flowers. Terpenes and mono unsaturated hydrocarbons were quantified as major constituents of the volatile fraction in flowers (17.0 to 52.6%) and (13.7 to 20.0%). Organic acids were detected in both leaves and flowers with a percentage ranging from 3.3% to 32.0%. Aldehydes were also detected in leaves (12.6 to 41.4%) and in flowers (1.4 to 5.1%). The GC/MS analyzes allowed alcohols to be detected in leaves (20.6 to 42.9%) and in flowers (8.2 to 18.1%). These compounds represent the most important feature of the large Passiflorafamily. Moreover, a critical role in the coevolved mechanisms of pollinators' interaction has been investigated.

Published
2021
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48. Author Correction: Biallelic mutations in SORDcause a common and potentially treatable hereditary neuropathy with implications for diabetes

Author

Cortese, Andrea, Zhu, Yi, Rebelo, Adriana P., Negri, Sara, Courel, Steve, Abreu, Lisa, Bacon, Chelsea J., Bai, Yunhong, Bis-Brewer, Dana M., Bugiardini, Enrico, Buglo, Elena, Danzi, Matt C., Feely, Shawna M. E., Athanasiou-Fragkouli, Alkyoni, Haridy, Nourelhoda A., Isasi, Rosario, Khan, Alaa, Laurà, Matilde, Magri, Stefania, Pipis, Menelaos, Pisciotta, Chiara, Powell, Eric, Rossor, Alexander M., Saveri, Paola, Sowden, Janet E., Tozza, Stefano, Vandrovcova, Jana, Dallman, Julia, Grignani, Elena, Marchioni, Enrico, Scherer, Steven S., Tang, Beisha, Lin, Zhiqiang, Al-Ajmi, Abdullah, Schüle, Rebecca, Synofzik, Matthis, Maisonobe, Thierry, Stojkovic, Tanya, Auer-Grumbach, Michaela, Abdelhamed, Mohamed A., Hamed, Sherifa A., Zhang, Ruxu, Manganelli, Fiore, Santoro, Lucio, Taroni, Franco, Pareyson, Davide, Houlden, Henry, Herrmann, David N., Reilly, Mary M., Shy, Michael E., Zhai, R. Grace, and Zuchner, Stephan

Abstract

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Published
2020
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49. Paclitaxel-Loaded Silk Fibroin Nanoparticles: Method Validation by UHPLC-MS/MS to Assess an Exogenous Approach to Load Cytotoxic Drugs.

Author

Perteghella, Sara, Sottani, Cristina, Coccè, Valentina, Negri, Sara, Cavicchini, Loredana, Alessandri, Giulio, Cottica, Danilo, Torre, Maria Luisa, Grignani, Elena, and Pessina, Augusto

Subjects
SILK fibroin, PACLITAXEL, ANTINEOPLASTIC agents, TANDEM mass spectrometry, NANOPARTICLES, LIQUID chromatography
Abstract

The aim of this work was to load an anticancer drug, paclitaxel (PTX), on Silk Fibroin Nanoparticles (SFNs) by using an exogenous approach. SFNs were produced, freeze-dried and then loaded with PTX. An exogenous method allowed us to reduce both drug loss and environmental impact. In order to quantify PTX loaded in SFNs, a simple and reliable method using reversed phase liquid chromatography coupled to tandem mass spectrometry (rp-UHPLC-MS/MS) was developed. This methodology was validated by the determination of spiked QC samples in three consecutive days. Good accuracy and precision of the method were obtained, while the intra-day and inter-day precisions were less than 10.3%. For PTX, the limit of quantitation (LOQ) was 5.0 ng/mL. Recovery from the matrix (SFNs-PTX pellets) was calculated (81.2% at LOQ value) as PTX was entrapped in a new matrix like the polymer silk fibroin-based. This method was successfully applied to determine the encapsulation efficiency (1.00 ± 0.19%) and the nanoparticle loading (0.12 ± 0.02% w/w). The in vitro anticancer activity of SFNs-PTX was tested against CFPAC-1 cancer cells; results demonstrated a very high cytotoxic activity of SFNs-PTX, with a dose dependent inhibition of CFPAC-1 proliferation, confirmed by the IC50 value of 3450 ± 750 ng/mL. [ABSTRACT FROM AUTHOR]

Published
2019
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