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1. Determinants for antimicrobial resistance genes in farm dust on 333 poultry and pig farms in nine European countries

Author

Luiken, Roosmarijn EC., Heederik, Dick JJ., Scherpenisse, Peter, Van Gompel, Liese, van Heijnsbergen, Eri, Greve, Gerdit D., Jongerius-Gortemaker, Betty GM., Tersteeg-Zijderveld, Monique HG., Fischer, Jennie, Juraschek, Katharina, Skarżyńska, Magdalena, Zając, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A., Smit, Lidwien AM., Wouters, Inge M., Mevius, Dik J., and Schmitt, Heike

Published
2022
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2. Description and determinants of the faecal resistome and microbiome of farmers and slaughterhouse workers: A metagenome-wide cross-sectional study

Author

Van Gompel, Liese, Luiken, Roosmarijn E.C., Hansen, Rasmus B., Munk, Patrick, Bouwknegt, Martijn, Heres, Lourens, Greve, Gerdit D., Scherpenisse, Peter, Jongerius-Gortemaker, Betty G.M., Tersteeg-Zijderveld, Monique H.G., García-Cobos, Silvia, Dohmen, Wietske, Dorado-García, Alejandro, Wagenaar, Jaap A., Urlings, Bert A.P., Aarestrup, Frank M., Mevius, Dik J., Heederik, Dick J.J., Schmitt, Heike, Bossers, Alex, and Smit, Lidwien A.M.

Published
2020
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3. Determinants for antimicrobial resistance genes in farm dust on 333 poultry and pig farms in nine European countries

Author

Luiken, Roosmarijn Ec, Heederik, Dick Jj, Scherpenisse, Peter, Van Gompel, Liese, van Heijnsbergen, Eri, Greve, Gerdit D, Jongerius-Gortemaker, Betty Gm, Tersteeg-Zijderveld, Monique Hg, Fischer, Jennie, Juraschek, Katharina, Skarżyńska, Magdalena, Zając, Magdalena, Wasyl, Dariusz, EFFORT Group, Wagenaar, Jaap A, Smit, Lidwien Am, Wouters, Inge M, Mevius, Dik J, Schmitt, Heike, Klinische infectiologie en microb. lab., dIRAS RA-I&I I&I, Faculteit Diergeneeskunde, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I RA, LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, and dI&I I&I-4

Subjects
Pig, Livestock, Risk factors, Environmental Science(all), Dust, Environment, Antimicrobial resistance, Biochemistry, Poultry, One health
Abstract

Livestock feces with antimicrobial resistant bacteria reaches the farm floor, manure pit, farm land and wider environment by run off and aerosolization. Little research has been done on the role of dust in the spread of antimicrobial resistance (AMR) in farms. Concentrations and potential determinants of antimicrobial resistance genes (ARGs) in farm dust are at present not known. Therefore in this study absolute ARG levels, representing the levels people and animals might be exposed to, and relative abundances of ARGs, representing the levels in the bacterial population, were quantified in airborne farm dust using qPCR. Four ARGs were determined in 947 freshly settled farm dust samples, captured with electrostatic dustfall collectors (EDCs), from 174 poultry (broiler) and 159 pig farms across nine European countries. By using linear mixed modeling, associations with fecal ARG levels, antimicrobial use (AMU) and farm and animal related parameters were determined. Results show similar relative abundances in farm dust as in feces and a significant positive association (ranging between 0.21 and 0.82) between the two reservoirs. AMU in pigs was positively associated with ARG abundances in dust from the same stable. Higher biosecurity standards were associated with lower relative ARG abundances in poultry and higher relative ARG abundances in pigs. Lower absolute ARG levels in dust were driven by, among others, summer season and certain bedding materials for poultry, and lower animal density and summer season for pigs. This study indicates different pathways that contribute to shaping the dust resistome in livestock farms, related to dust generation, or affecting the bacterial microbiome. Farm dust is a large reservoir of ARGs from which transmission to bacteria in other reservoirs can possibly occur. The identified determinants of ARG abundances in farm dust can guide future research and potentially farm management policy.

Published
2022

4. Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries

Author

Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, Schmitt, Heike, Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, and Schmitt, Heike

Abstract

OBJECTIVES: The occurrence and zoonotic potential of antimicrobial resistance (AMR) in pigs and broilers has been studied intensively in past decades. Here, we describe AMR levels of European pig and broiler farms and determine the potential risk factors.METHODS: We collected faeces from 181 pig farms and 181 broiler farms in nine European countries. Real-time quantitative PCR (qPCR) was used to quantify the relative abundance of four antimicrobial resistance genes (ARGs) [aph(3')-III, erm(B), sul2 and tet(W)] in these faeces samples. Information on antimicrobial use (AMU) and other farm characteristics was collected through a questionnaire. A mixed model using country and farm as random effects was performed to evaluate the relationship of AMR with AMU and other farm characteristics. The correlation between individual qPCR data and previously published pooled metagenomic data was evaluated. Variance component analysis was conducted to assess the variance contribution of all factors.RESULTS: The highest abundance of ARG was for tet(W) in pig faeces and erm(B) in broiler faeces. In addition to the significant positive association between corresponding ARG and AMU levels, we also found on-farm biosecurity measures were associated with relative ARG abundance in both pigs and broilers. Between-country and between-farm variation can partially be explained by AMU. Different ARG targets may have different sample size requirements to represent the overall farm level precisely.CONCLUSIONS: qPCR is an efficient tool for targeted assessment of AMR in livestock-related samples. The AMR variation between samples was mainly contributed to by between-country, between-farm and within-farm differences, and then by on-farm AMU.

Published
2022

5. Determinants for antimicrobial resistance genes in farm dust on 333 poultry and pig farms in nine European countries

Author

Klinische infectiologie en microb. lab., dIRAS RA-I&I I&I, Faculteit Diergeneeskunde, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I RA, LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, dI&I I&I-4, Luiken, Roosmarijn Ec, Heederik, Dick Jj, Scherpenisse, Peter, Van Gompel, Liese, van Heijnsbergen, Eri, Greve, Gerdit D, Jongerius-Gortemaker, Betty Gm, Tersteeg-Zijderveld, Monique Hg, Fischer, Jennie, Juraschek, Katharina, Skarżyńska, Magdalena, Zając, Magdalena, Wasyl, Dariusz, EFFORT Group, Wagenaar, Jaap A, Smit, Lidwien Am, Wouters, Inge M, Mevius, Dik J, Schmitt, Heike, Klinische infectiologie en microb. lab., dIRAS RA-I&I I&I, Faculteit Diergeneeskunde, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I RA, LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, dI&I I&I-4, Luiken, Roosmarijn Ec, Heederik, Dick Jj, Scherpenisse, Peter, Van Gompel, Liese, van Heijnsbergen, Eri, Greve, Gerdit D, Jongerius-Gortemaker, Betty Gm, Tersteeg-Zijderveld, Monique Hg, Fischer, Jennie, Juraschek, Katharina, Skarżyńska, Magdalena, Zając, Magdalena, Wasyl, Dariusz, EFFORT Group, Wagenaar, Jaap A, Smit, Lidwien Am, Wouters, Inge M, Mevius, Dik J, and Schmitt, Heike

Published
2022

6. Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries

Author

IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, dIRAS RA-I&I RA, Klinische infectiologie en microb. lab., dI&I I&I-4, dIRAS RA-I&I I&I, LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, Schmitt, Heike, IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, dIRAS RA-I&I RA, Klinische infectiologie en microb. lab., dI&I I&I-4, dIRAS RA-I&I I&I, LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, and Schmitt, Heike

Published
2022

7. Antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) abundance in animal faeces, meat, production environments and human faeces in Europe

Author

IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, dIRAS RA-I&I RA, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, IRAS OH Epidemiology Chemical Agents, dIRAS RA-2, dI&I I&I-4, Yang, Dongsheng, Heederik, Dick J J, Scherpenisse, Peter, Van Gompel, Liese, Luiken, Roosmarijn E C, Wadepohl, Katharina, Skarżyńska, Magdalena, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Portengen, Lützen, Juraschek, Katharina, Fischer, Jennie, Zając, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Mevius, Dik J, Smit, Lidwien A M, Schmitt, Heike, IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, dIRAS RA-I&I RA, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, IRAS OH Epidemiology Chemical Agents, dIRAS RA-2, dI&I I&I-4, Yang, Dongsheng, Heederik, Dick J J, Scherpenisse, Peter, Van Gompel, Liese, Luiken, Roosmarijn E C, Wadepohl, Katharina, Skarżyńska, Magdalena, Van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Portengen, Lützen, Juraschek, Katharina, Fischer, Jennie, Zając, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Mevius, Dik J, Smit, Lidwien A M, and Schmitt, Heike

Published
2022

8. Determinants for antimicrobial resistance genes in farm dust on 333 poultry and pig farms in nine European countries

Author

Luiken, Roosmarijn E.C., Heederik, Dick J.J., Scherpenisse, Peter, Van Gompel, Liese, van Heijnsbergen, Eri, Greve, Gerdit D., Jongerius-Gortemaker, Betty G.M., Tersteeg-Zijderveld, Monique H.G., Fischer, Jennie, Juraschek, Katharina, Skarżyńska, Magdalena, Zając, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A., Smit, Lidwien A.M., Wouters, Inge M., Mevius, Dik J., Schmitt, Heike, Luiken, Roosmarijn E.C., Heederik, Dick J.J., Scherpenisse, Peter, Van Gompel, Liese, van Heijnsbergen, Eri, Greve, Gerdit D., Jongerius-Gortemaker, Betty G.M., Tersteeg-Zijderveld, Monique H.G., Fischer, Jennie, Juraschek, Katharina, Skarżyńska, Magdalena, Zając, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A., Smit, Lidwien A.M., Wouters, Inge M., Mevius, Dik J., and Schmitt, Heike

Abstract

Livestock feces with antimicrobial resistant bacteria reaches the farm floor, manure pit, farm land and wider environment by run off and aerosolization. Little research has been done on the role of dust in the spread of antimicrobial resistance (AMR) in farms. Concentrations and potential determinants of antimicrobial resistance genes (ARGs) in farm dust are at present not known. Therefore in this study absolute ARG levels, representing the levels people and animals might be exposed to, and relative abundances of ARGs, representing the levels in the bacterial population, were quantified in airborne farm dust using qPCR. Four ARGs were determined in 947 freshly settled farm dust samples, captured with electrostatic dustfall collectors (EDCs), from 174 poultry (broiler) and 159 pig farms across nine European countries. By using linear mixed modeling, associations with fecal ARG levels, antimicrobial use (AMU) and farm and animal related parameters were determined. Results show similar relative abundances in farm dust as in feces and a significant positive association (ranging between 0.21 and 0.82) between the two reservoirs. AMU in pigs was positively associated with ARG abundances in dust from the same stable. Higher biosecurity standards were associated with lower relative ARG abundances in poultry and higher relative ARG abundances in pigs. Lower absolute ARG levels in dust were driven by, among others, summer season and certain bedding materials for poultry, and lower animal density and summer season for pigs. This study indicates different pathways that contribute to shaping the dust resistome in livestock farms, related to dust generation, or affecting the bacterial microbiome. Farm dust is a large reservoir of ARGs from which transmission to bacteria in other reservoirs can possibly occur. The identified determinants of ARG abundances in farm dust can guide future research and potentially farm management policy.

Published
2022

9. Antimicrobial resistance genes aph(3′)-III, erm(B), sul2 and tet(W) abundance in animal faeces, meat, production environments and human faeces in Europe

Author

Yang, Dongsheng, primary, Heederik, Dick J J, additional, Scherpenisse, Peter, additional, Van Gompel, Liese, additional, Luiken, Roosmarijn E C, additional, Wadepohl, Katharina, additional, Skarżyńska, Magdalena, additional, Van Heijnsbergen, Eri, additional, Wouters, Inge M, additional, Greve, Gerdit D, additional, Jongerius-Gortemaker, Betty G M, additional, Tersteeg-Zijderveld, Monique, additional, Portengen, Lützen, additional, Juraschek, Katharina, additional, Fischer, Jennie, additional, Zając, Magdalena, additional, Wasyl, Dariusz, additional, Wagenaar, Jaap A, additional, Mevius, Dik J, additional, Smit, Lidwien A M, additional, and Schmitt, Heike, additional

Published
2022
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10. Association of antimicrobial usage with faecal abundance of aph(3’)-III, ermB, sul2 and tetW resistance genes in veal calves in three European countries

Author

Yang, Dongsheng, Van Gompel, Liese, Luiken, Roosmarijn E.C., Sanders, Pim, Joosten, Philip, van Heijnsbergen, Eri, Wouters, Inge M., Scherpenisse, Peter, Chauvin, Claire, Wadepohl, Katharina, Greve, Gerdit D., Jongerius-Gortemaker, Betty G.M., Tersteeg-Zijderveld, Monique H.G., Soumet, Christophe, Skarżyńska, Magdalena, Juraschek, Katharina, Fischer, Jennie, Wasyl, Dariusz, Wagenaar, Jaap A., Dewulf, Jeroen, Schmitt, Heike, Mevius, Dik J., Heederik, Dick J.J., and Smit, Lidwien A.M.

Published
2020
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13. Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers

Author

Van Gompel, Liese, Dohmen, Wietske, Luiken, Roosmarijn E C, Bouwknegt, Martijn, Heres, Lourens, Van Heijnsbergen, Eri, Jongerius-Gortemaker, Betty G M, Scherpenisse, Peter, Greve, Gerdit D, Tersteeg-Zijderveld, Monique H G, Wadepohl, Katharina, Ribeiro Duarte, Ana Sofia, Muñoz-Gómez, Violeta, Fischer, Jennie, Skarżyńska, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Urlings, Bert A P, Dorado-García, Alejandro, Wouters, Inge M, Heederik, Dick J J, Schmitt, Heike, Smit, Lidwien A M, Van Gompel, Liese, Dohmen, Wietske, Luiken, Roosmarijn E C, Bouwknegt, Martijn, Heres, Lourens, Van Heijnsbergen, Eri, Jongerius-Gortemaker, Betty G M, Scherpenisse, Peter, Greve, Gerdit D, Tersteeg-Zijderveld, Monique H G, Wadepohl, Katharina, Ribeiro Duarte, Ana Sofia, Muñoz-Gómez, Violeta, Fischer, Jennie, Skarżyńska, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Urlings, Bert A P, Dorado-García, Alejandro, Wouters, Inge M, Heederik, Dick J J, Schmitt, Heike, and Smit, Lidwien A M

Abstract

OBJECTIVES: Slaughterhouse staff is occupationally exposed to antimicrobial resistant bacteria. Studies reported high antimicrobial resistance gene (ARG) abundances in slaughter pigs. This cross-sectional study investigated occupational exposure to tetracycline (tetW) and macrolide (ermB) resistance genes and assessed determinants for faecal tetW and ermB carriage among pig slaughterhouse workers.METHODS: During 2015-2016, 483 faecal samples and personal questionnaires were collected from workers in a Dutch pig abattoir, together with 60 pig faecal samples. Human dermal and respiratory exposure was assessed by examining 198 carcass, 326 gloves, and 33 air samples along the line, next to 198 packed pork chops to indicate potential consumer exposure. Samples were analyzed by qPCR (tetW, ermB). A job exposure matrix was created by calculating the percentage of tetW and ermB positive carcasses or gloves for each job position. Multiple linear regression models were used to link exposure to tetW and ermB carriage.RESULTS: Workers are exposed to tetracycline and macrolide resistance genes along the slaughter line. Tetw and ermB gradients were found for carcasses, gloves, and air filters. One packed pork chop contained tetW, ermB was non-detectable. Human faecal tetW and ermB concentrations were lower than in pig faeces. Associations were found between occupational tetW exposure and human faecal tetW carriage, yet, not after model adjustments. Sampling round, nationality, and smoking were determinants for ARG carriage.CONCLUSION: We demonstrated clear environmental tetracycline and macrolide resistance gene exposure gradients along the slaughter line. No robust link was found between ARG exposure and human faecal ARG carriage.

Published
2020

14. Description and determinants of the faecal resistome and microbiome of farmers and slaughterhouse workers : A metagenome-wide cross-sectional study

Author

Gompel, Liese, Van, Luiken, Roosmarijn E.C., Hansen, Rasmus B., Munk, Patrick, Bouwknegt, Martijn, Heres, Lourens, Greve, Gerdit D., Scherpenisse, Peter, Jongerius-Gortemaker, Betty G.M., Tersteeg-Zijderveld, Monique H.G., García-Cobos, Silvia, Dohmen, Wietske, Dorado-García, Alejandro, Wagenaar, Jaap A., Urlings, Bert A.P., Aarestrup, Frank M., Mevius, Dik J., Heederik, Dick J.J., Schmitt, Heike, Bossers, Alex, Smit, Lidwien A.M., Gompel, Liese, Van, Luiken, Roosmarijn E.C., Hansen, Rasmus B., Munk, Patrick, Bouwknegt, Martijn, Heres, Lourens, Greve, Gerdit D., Scherpenisse, Peter, Jongerius-Gortemaker, Betty G.M., Tersteeg-Zijderveld, Monique H.G., García-Cobos, Silvia, Dohmen, Wietske, Dorado-García, Alejandro, Wagenaar, Jaap A., Urlings, Bert A.P., Aarestrup, Frank M., Mevius, Dik J., Heederik, Dick J.J., Schmitt, Heike, Bossers, Alex, and Smit, Lidwien A.M.

Abstract

Background: By studying the entire human faecal resistome and associated microbiome, the diversity and abundance of faecal antimicrobial resistance genes (ARGs) can be comprehensively characterized. Prior culture-based studies have shown associations between occupational exposure to livestock and carriage of specific antimicrobial resistant bacteria. Using shotgun metagenomics, the present study investigated 194 faecal resistomes and bacteriomes from humans occupationally exposed to ARGs in livestock (i.e. pig and poultry farmers, employees and family members and pig slaughterhouse workers) and a control population (Lifelines cohort) in the Netherlands. In addition, we sought to identify determinants for the human resistome and bacteriome composition by applying a combination of multivariate (NMDS, PERMANOVA, SIMPER and DESeq2 analysis) and multivariable regression analysis techniques. Results: Pig slaughterhouse workers and pig farmers carried higher total ARG abundances in their stools compared to broiler farmers and control subjects. Tetracycline, β-lactam and macrolide resistance gene clusters dominated the resistome of all studied groups. No significant resistome alpha diversity differences were found among the four populations. However, the resistome beta diversity showed a separation of the mean resistome composition of pig and pork exposed workers from broiler farmers and controls, independent of their antimicrobial use. We demonstrated differences in resistome composition between slaughter line positions, pig versus poultry exposed workers, as well as differences between farmers and employees versus family members. In addition, we found a significant correlation between the bacteriome and resistome, and significant differences in the bacteriome composition between and within the studied subpopulations. Finally, an in-depth analysis of pig and poultry farms – of which also farm livestock resistomes were analysed – showed positive associations between the

Published
2020

15. Description and determinants of the faecal resistome and microbiome of farmers and slaughterhouse workers: A metagenome-wide cross-sectional study

Author

IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., One Health Microbieel, dIRAS RA-I&I RA, dI&I I&I-4, Faculteit Diergeneeskunde, Van Gompel, Liese, Luiken, Roosmarijn E C, Hansen, Rasmus B, Munk, Patrick, Bouwknegt, Martijn, Heres, Lourens, Greve, Gerdit D, Scherpenisse, Peter, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique H G, García-Cobos, Silvia, Dohmen, Wietske, Dorado-García, Alejandro, Wagenaar, Jaap A, Urlings, Bert A P, Aarestrup, Frank M, Mevius, Dik J, Heederik, Dick J J, Schmitt, Heike, Bossers, Alex, Smit, Lidwien A M, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., One Health Microbieel, dIRAS RA-I&I RA, dI&I I&I-4, Faculteit Diergeneeskunde, Van Gompel, Liese, Luiken, Roosmarijn E C, Hansen, Rasmus B, Munk, Patrick, Bouwknegt, Martijn, Heres, Lourens, Greve, Gerdit D, Scherpenisse, Peter, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique H G, García-Cobos, Silvia, Dohmen, Wietske, Dorado-García, Alejandro, Wagenaar, Jaap A, Urlings, Bert A P, Aarestrup, Frank M, Mevius, Dik J, Heederik, Dick J J, Schmitt, Heike, Bossers, Alex, and Smit, Lidwien A M

Published
2020

16. Association of antimicrobial usage with faecal abundance of aph(3')-III, ermB, sul2 and tetW resistance genes in veal calves in three European countries

Author

IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, One Health Microbieel, dI&I I&I-4, Faculteit Diergeneeskunde, Yang, Dongsheng, Van Gompel, Liese, Luiken, Roosmarijn E C, Sanders, Pim, Joosten, Philip, van Heijnsbergen, Eri, Wouters, Inge M, Scherpenisse, Peter, Chauvin, Claire, Wadepohl, Katharina, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Soumet, Christophe, Skarżyńska, Magdalena, Juraschek, Katharina, Fischer, Jennie, Wasyl, Dariusz, Wagenaar, Jaap A, Dewulf, Jeroen, Schmitt, Heike, Mevius, Dik J, Heederik, Dick J J, Smit, Lidwien A M, IRAS OH Epidemiology Microbial Agents, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, One Health Microbieel, dI&I I&I-4, Faculteit Diergeneeskunde, Yang, Dongsheng, Van Gompel, Liese, Luiken, Roosmarijn E C, Sanders, Pim, Joosten, Philip, van Heijnsbergen, Eri, Wouters, Inge M, Scherpenisse, Peter, Chauvin, Claire, Wadepohl, Katharina, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Soumet, Christophe, Skarżyńska, Magdalena, Juraschek, Katharina, Fischer, Jennie, Wasyl, Dariusz, Wagenaar, Jaap A, Dewulf, Jeroen, Schmitt, Heike, Mevius, Dik J, Heederik, Dick J J, and Smit, Lidwien A M

Published
2020

17. Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers

Author

One Health Microbieel, dIRAS RA-I&I I&I, LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, LS Klinisch Onderzoek Wagenaar, dI&I I&I-4, Faculteit Diergeneeskunde, Van Gompel, Liese, Dohmen, Wietske, Luiken, Roosmarijn E C, Bouwknegt, Martijn, Heres, Lourens, Van Heijnsbergen, Eri, Jongerius-Gortemaker, Betty G M, Scherpenisse, Peter, Greve, Gerdit D, Tersteeg-Zijderveld, Monique H G, Wadepohl, Katharina, Ribeiro Duarte, Ana Sofia, Muñoz-Gómez, Violeta, Fischer, Jennie, Skarżyńska, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Urlings, Bert A P, Dorado-García, Alejandro, Wouters, Inge M, Heederik, Dick J J, Schmitt, Heike, Smit, Lidwien A M, One Health Microbieel, dIRAS RA-I&I I&I, LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, LS Klinisch Onderzoek Wagenaar, dI&I I&I-4, Faculteit Diergeneeskunde, Van Gompel, Liese, Dohmen, Wietske, Luiken, Roosmarijn E C, Bouwknegt, Martijn, Heres, Lourens, Van Heijnsbergen, Eri, Jongerius-Gortemaker, Betty G M, Scherpenisse, Peter, Greve, Gerdit D, Tersteeg-Zijderveld, Monique H G, Wadepohl, Katharina, Ribeiro Duarte, Ana Sofia, Muñoz-Gómez, Violeta, Fischer, Jennie, Skarżyńska, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Urlings, Bert A P, Dorado-García, Alejandro, Wouters, Inge M, Heederik, Dick J J, Schmitt, Heike, and Smit, Lidwien A M

Published
2020

18. Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers

Author

Van Gompel, Liese, primary, Dohmen, Wietske, additional, Luiken, Roosmarijn E C, additional, Bouwknegt, Martijn, additional, Heres, Lourens, additional, van Heijnsbergen, Eri, additional, Jongerius-Gortemaker, Betty G M, additional, Scherpenisse, Peter, additional, Greve, Gerdit D, additional, Tersteeg-Zijderveld, Monique H G, additional, Wadepohl, Katharina, additional, Ribeiro Duarte, Ana Sofia, additional, Muñoz-Gómez, Violeta, additional, Fischer, Jennie, additional, Skarżyńska, Magdalena, additional, Wasyl, Dariusz, additional, Wagenaar, Jaap A, additional, Urlings, Bert A P, additional, Dorado-García, Alejandro, additional, Wouters, Inge M, additional, Heederik, Dick J J, additional, Schmitt, Heike, additional, and Smit, Lidwien A M, additional

Published
2019
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19. Occupational Exposure and Carriage of Antimicrobial Resistance Genes (tetW, ermB) in Pig Slaughterhouse Workers.

Author

Gompel, Liese Van, Dohmen, Wietske, Luiken, Roosmarijn E C, Bouwknegt, Martijn, Heres, Lourens, Heijnsbergen, Eri van, Jongerius-Gortemaker, Betty G M, Scherpenisse, Peter, Greve, Gerdit D, Tersteeg-Zijderveld, Monique H G, Wadepohl, Katharina, Duarte, Ana Sofia Ribeiro, Muñoz-Gómez, Violeta, Fischer, Jennie, Skarżyńska, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A, Urlings, Bert A P, Dorado-García, Alejandro, and Wouters, Inge M

Subjects
FECAL analysis, DRUG resistance in microorganisms, FOOD industry, GENES, MACROLIDE antibiotics, POLYMERASE chain reaction, QUESTIONNAIRES, SWINE, TETRACYCLINE, OCCUPATIONAL hazards, ENVIRONMENTAL exposure, MULTIPLE regression analysis, CROSS-sectional method
Abstract

Objectives Slaughterhouse staff is occupationally exposed to antimicrobial resistant bacteria. Studies reported high antimicrobial resistance gene (ARG) abundances in slaughter pigs. This cross-sectional study investigated occupational exposure to tetracycline (tetW) and macrolide (ermB) resistance genes and assessed determinants for faecal tetW and ermB carriage among pig slaughterhouse workers. Methods During 2015–2016, 483 faecal samples and personal questionnaires were collected from workers in a Dutch pig abattoir, together with 60 pig faecal samples. Human dermal and respiratory exposure was assessed by examining 198 carcass, 326 gloves, and 33 air samples along the line, next to 198 packed pork chops to indicate potential consumer exposure. Samples were analyzed by qPCR (tetW , ermB). A job exposure matrix was created by calculating the percentage of tetW and ermB positive carcasses or gloves for each job position. Multiple linear regression models were used to link exposure to tetW and ermB carriage. Results Workers are exposed to tetracycline and macrolide resistance genes along the slaughter line. Tetw and ermB gradients were found for carcasses, gloves, and air filters. One packed pork chop contained tetW , ermB was non-detectable. Human faecal tetW and ermB concentrations were lower than in pig faeces. Associations were found between occupational tetW exposure and human faecal tetW carriage, yet, not after model adjustments. Sampling round, nationality, and smoking were determinants for ARG carriage. Conclusion We demonstrated clear environmental tetracycline and macrolide resistance gene exposure gradients along the slaughter line. No robust link was found between ARG exposure and human faecal ARG carriage. [ABSTRACT FROM AUTHOR]

Published
2020
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25. Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries.

Author

Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, Schmitt, Heike, Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, and Schmitt, Heike

26. Risk factors for the abundance of antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) in pig and broiler faeces in nine European countries.

Author

Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, Schmitt, Heike, Yang, Dongsheng, Heederik, Dick J J, Mevius, Dik J, Scherpenisse, Peter, Luiken, Roosmarijn E C, Van Gompel, Liese, Skarżyńska, Magdalena, Wadepohl, Katharina, Chauvin, Claire, van Heijnsbergen, Eri, Wouters, Inge M, Greve, Gerdit D, Jongerius-Gortemaker, Betty G M, Tersteeg-Zijderveld, Monique, Zając, Magdalena, Wasyl, Dariusz, Juraschek, Katharina, Fischer, Jennie, Wagenaar, Jaap A, Smit, Lidwien A M, and Schmitt, Heike

27. Variables determining the impact of diazinon on aquatic insects: taxon, developmental stage, and exposure time

Author

Kraak, Michiel H. S., Greve, Gerdit D., van der Geest, Harm G., Stuijfzand, Suzanne C., and Poort, Liesbeth

Subjects
*AQUACULTURE, *WATER pollution monitoring, *DIAZINON, *TOXICITY testing, *AQUATIC insects, *AGRICULTURE, *PESTICIDES
Abstract

Several variables determine the impact of a pesticide on aquatic invertebrates. In this study, aquatic insects were subjected to the common insecticide diazinon; we analyzed the variables taxon, developmental stage, and exposure time. Effects of diazinon on the caddis fly Hydropsyche angustipennis and the midge Chironomus riparius were determined in the laboratory during different exposure times (48 and 96 h) using mortality, activity, and growth as end points. Last instars of both species displayed a clear behavioral response at concentrations much lower than those affecting survival. Doubling the exposure time from 2 to 4 d decreased survival of midges and caddis flies by a factor 1.4 to 8.4. The 96-h 50% lethal concentrations were: 1.3 mug/L (first instar of the caddis fly), 29 mug/L (fifth instar of the caddis fly), 23 mug/L (first instar of the midge) and 167 mug/L (fourth instar of the midge). Within the spectrum of tested insects (nine species for which 48-h 50% lethal concentrations have been reported in the literature), H. angustipennis is the second most sensitive, and C. riparius the most tolerant species. However, the ranking of species strongly depends on the developmental stage; differences between species are often smaller than differences between instars of one species. Thelarge difference in sensitivities between young and old larvae implythat the impact of a pesticide strongly depends on the season of occurrence. Runoff from pesticide applications on crops is more likely to occur in spring and summer and may have a relatively greater impacton insect communities since young larvae prevail in these seasons. In addition, recovery of typical riverine insects such as H. angustipennis from incidental exposure will be slow, considering their relatively long life cycle. [ABSTRACT FROM AUTHOR]

Published
2000

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