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3. The genome and lifestage-specific transcriptomes of a plant-parasitic nematode and its host reveal susceptibility genes involved in trans-kingdom synthesis of vitamin B5

Author

Siddique, Shahid, Radakovic, Zoran S, Hiltl, Clarissa, Pellegrin, Clement, Baum, Thomas J, Beasley, Helen, Bent, Andrew F, Chitambo, Oliver, Chopra, Divykriti, Danchin, Etienne GJ, Grenier, Eric, Habash, Samer S, Hasan, M Shamim, Helder, Johannes, Hewezi, Tarek, Holbein, Julia, Holterman, Martijn, Janakowski, Sławomir, Koutsovoulos, Georgios D, Kranse, Olaf P, Lozano-Torres, Jose L, Maier, Tom R, Masonbrink, Rick E, Mendy, Badou, Riemer, Esther, Sobczak, Mirosław, Sonawala, Unnati, Sterken, Mark G, Thorpe, Peter, van Steenbrugge, Joris JM, Zahid, Nageena, Grundler, Florian, and Eves-van den Akker, Sebastian

Subjects
Veterinary Sciences, Agricultural, Veterinary and Food Sciences, Biological Sciences, Human Genome, Genetics, Prevention, Nutrition, Infectious Diseases, Aetiology, 2.2 Factors relating to the physical environment, Infection, Animals, Parasites, Pantothenic Acid, Transcriptome, Tylenchida, Cysts
Abstract

Plant-parasitic nematodes are a major threat to crop production in all agricultural systems. The scarcity of classical resistance genes highlights a pressing need to find new ways to develop nematode-resistant germplasm. Here, we sequence and assemble a high-quality phased genome of the model cyst nematode Heterodera schachtii to provide a platform for the first system-wide dual analysis of host and parasite gene expression over time, covering all major parasitism stages. Analysis of the hologenome of the plant-nematode infection site identified metabolic pathways that were incomplete in the parasite but complemented by the host. Using a combination of bioinformatic, genetic, and biochemical approaches, we show that a highly atypical completion of vitamin B5 biosynthesis by the parasitic animal, putatively enabled by a horizontal gene transfer from a bacterium, is required for full pathogenicity. Knockout of either plant-encoded or now nematode-encoded steps in the pathway significantly reduces parasitic success. Our experiments establish a reference for cyst nematodes, further our understanding of the evolution of plant-parasitism by nematodes, and show that congruent differential expression of metabolic pathways in the infection hologenome represents a new way to find nematode susceptibility genes. The approach identifies genome-editing-amenable targets for future development of nematode-resistant crops.

Published
2022

10. Protective Geometry and Reproductive Anatomy as Candidate Determinants of Clutch Size Variation in Pentatomid Bugs

Author

Abram, Paul K., primary, Guerra-Grenier, Eric, additional, Brodeur, Jacques, additional, Capko, Clarissa, additional, Aquino, Michely Ferreira Santos, additional, Beers, Elizabeth H., additional, Blassioli-Moraes, Maria Carolina, additional, Borges, Miguel, additional, Cingolani, M. Fernanda, additional, Cusumano, Antonino, additional, De Clercq, Patrick, additional, Fernandez, Celina A., additional, Gariepy, Tara D., additional, Haye, Tim, additional, Hoelmer, Kim, additional, Laumann, Raul Alberto, additional, Lietti, Marcela, additional, McPherson, J. E., additional, Punschke, Eduardo, additional, Saunders, Thomas E., additional, Zhang, Jin-Ping, additional, and Hardy, Ian C. W., additional

Published
2023
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16. Protective geometry and reproductive anatomy as candidate determinants of clutch size variation in pentatomid bugs

Author

Abram, Paul K, primary, Guerra-Grenier, Eric, additional, Brodeur, Jacques, additional, Capko, Clarissa, additional, Aquino, Michely Ferreira Santos, additional, Beers, Betsy, additional, Blassioli-Moraes, Maria Carolina, additional, Borges, Miguel, additional, Cingolani, M. Fernanda, additional, Cusumano, Antonino, additional, DeClercq, Patrick, additional, Fernandez, Celina A., additional, Gariepy, Tara D., additional, Haye, Tim, additional, Hoelmer, Kim, additional, Laumann, Raul Alberto, additional, Lietti, Marcela, additional, McPherson, J. E., additional, Punschke, Eduardo, additional, Saunders, Thomas E., additional, Zhang, Jin Ping, additional, and Hardy, Ian C.W., additional

Published
2022
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18. The genome and lifestage-specific transcriptomes of a plant-parasitic nematode and its host reveal susceptibility genes involved in trans-kingdom synthesis of vitamin B5

Author

Siddique, Shahid, primary, Radakovic, Zoran S., additional, Hiltl, Clarissa, additional, Pellegrin, Clement, additional, Baum, Thomas J., additional, Beasley, Helen, additional, Chitambo, Oliver, additional, Chopra, Divykriti, additional, Danchin, Etienne G.J., additional, Grenier, Eric, additional, Habash, Samer S., additional, Hasan, M. Shamim, additional, Helder, Johannes, additional, Hewezi, Tarek, additional, Holbein, Julia, additional, Holterman, Martijn, additional, Janakowski, Sławomir, additional, Koutsovoulos, Georgios D., additional, Kranse, Olaf P., additional, Lozano-Torres, Jose L., additional, Maier, Tom R., additional, Masonbrink, Rick E., additional, Mendy, Badou, additional, Riemer, Esther, additional, Sobczak, Mirosław, additional, Sonawala, Unnati, additional, Sterken, Mark G., additional, Thorpe, Peter, additional, van Steenbrugge, Joris J.M., additional, Zahid, Nageena, additional, Grundler, Florian, additional, and Eves-van den Akker, Sebastian, additional

Published
2021
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20. sj-pdf-1-ajs-10.1177_03635465211041593 – Supplemental material for A High-Sensitivity International Knee Documentation Committee Survey Index From the PROMIS System: The Next-Generation Patient-Reported Outcome for a Knee Injury Population

Author

Tenan, Matthew S., Robins, Richard J., Sheean, Andrew J., Dekker, Travis J., Bailey, James R., Bharmal, Husain M., Bradley, Matthew W., Cameron, Kenneth L., Burns, Travis C., Freedman, Brett A., Galvin, Joseph W., Grenier, Eric S., Haley, Chad A., Hurvitz, Andrew P., LeClere, Lance E., Lee, Ian, Mauntel, Timothy, McDonald, Lucas S., Nesti, Leon J., Owens, Brett D., Posner, Matthew A., Potter, Benjamin K., Provencher, Matthew T., Rhon, Daniel I., Roach, Christopher J., Ryan, Paul M., Schmitz, Matthew R., Slabaugh, Mark A., Tucker, Christopher J., Volk, William R., Dickens, Jonathan F., and Feagin, John A.

Subjects
FOS: Clinical medicine, 110323 Surgery, 110604 Sports Medicine, FOS: Health sciences, 110314 Orthopaedics
Abstract

Supplemental material, sj-pdf-1-ajs-10.1177_03635465211041593 for A High-Sensitivity International Knee Documentation Committee Survey Index From the PROMIS System: The Next-Generation Patient-Reported Outcome for a Knee Injury Population by Matthew S. Tenan, Richard J. Robins, Andrew J. Sheean, Travis J. Dekker, James R. Bailey, Husain M. Bharmal, Matthew W. Bradley, Kenneth L. Cameron, Travis C. Burns, Brett A. Freedman, Joseph W. Galvin, Eric S. Grenier, Chad A. Haley, Andrew P. Hurvitz, Lance E. LeClere, Ian Lee, Timothy Mauntel, Lucas S. McDonald, Leon J. Nesti, Brett D. Owens, Matthew A. Posner, Benjamin K. Potter, Matthew T. Provencher, Daniel I. Rhon, Christopher J. Roach, Paul M. Ryan, Matthew R. Schmitz, Mark A. Slabaugh, Christopher J. Tucker, William R. Volk and Jonathan F. Dickens in The American Journal of Sports Medicine

Published
2021
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23. Adaptation du nématode à kyste aux variétés résistantes

Author

Fournet, Sylvain, Eoche-Bosy, Delphine, Kerlan, Marie-Claire, Grenier, Eric, Montarry, Josselin, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Grenier, Anne-Sophie, Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2020

24. Development of innovative tools for the durable management of the risks associated with nematodes for seed potato production and other crops in rotation

Author

Le Roux, Anne-Claire, Neveux, M-S, Huchet, E., Dewaegeneire, Pauline, Berton, L., Demey’, l., Gobert, Virginie, Lehingrat, Y., Ollivier, François, Chappé, A-M, Hotte, H., Montarry, Josselin, Fournet, Sylvain, Esquibet, Magali, Fouville, Didier, Mille, B., Kerlan, Marie-Claire, Coléno, François-Christophe, Iachia, Catherine, Mulet, K.., Grenier, Eric, Castagnone, Philippe, Folcher, Laurent, Fédération Nationale des Producteurs de Plants de Pomme de Terre (FN3PT), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Sciences pour l'Action et le Développement : Activités, Produits, Territoires (SADAPT), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), ministère de l’agriculture via le compte d’affectation spéciale « Développement agricole et rural » dans le cadre de l’appel à projets Recherche Technologique., Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Université Nice Sophia Antipolis (... - 2019) (UNS)

Subjects
alternative control methods, detection, Analyse de risques phytosanitaires, waste disinfection, détection, nématodes, méthodes alternatives de lutte, Pest risk analysis (PRA), plantes de service, nematodes, [SDE]Environmental Sciences, sanitizing plants, désinfection de déchets
Abstract

Nematools project aimed at reinforcing prophylactic methods to prevent introduction and disseminationof regulated phytoparasites nematodes Meloidogyne chitwoodi, M. fallax, Globodera pallida and G.rostochiensis which are serious threats for potato crops and to improve the control strategies in order to preserve the privileged health status of the French territory. This project made it possible to developnew molecular tools for the detection of root-knot nematodes in soil samples, and later their validationon a collection of nematode populations established during the project. The risk analysis on thedissemination of nematodes related to agricultural practices have led firstly to the development of aconceptual model for the identification of management strategies for the use of agricultural equipment atrisk and on the other hand to highlight and to quantify the importance of cyst nematodes dispersal bythe soil practices and by soil-adhering to agricultural machinery at the field and at the farm scale. Theactions carried out in partnership with industrials and with experimental stations made it possible toproduce a user-friendly risk analysis tool characterizing the critical phases of different waste and effluenttreatment processes and providing answers on the sanitizing performances of different decontaminationprocesses such as composting, lagooning, anaerobic digestion, chlorination, and heat treatment. Thisproject also allowed to screen, under controlled conditions, plant species which are not conducive to thedevelopment and propagation of root-knot nematodes such as M. chitwoodi and M. fallax and may beproposed as rupture plants on contaminated sites; Les actions conduites dans le cadre du projet de recherche Nematools visaient à renforcer lesméthodes prophylactiques pour prévenir l’introduction et la dissémination des nématodesphytoparasites réglementés Meloidogyne chitwoodi, M. fallax, Globodera pallida et G. rostochiensis.Ces nématodes constituent des menaces sérieuses pour diverses cultures, dont la pomme de terre, etconduisent à repenser les stratégies de lutte afin de préserver l’état sanitaire privilégié du territoirenational. Le projet a permis le développement de nouveaux outils moléculaires de détection desnématodes à galle dans des échantillons de sol, outils validés sur une collection de populations denématodes constituée pendant la durée du projet. Les travaux portant sur l’analyse du risque dedissémination des nématodes, associés aux pratiques agricoles, ont conduit (1) à l’élaboration d’unmodèle conceptuel permettant d’identifier les stratégies de gestion du matériel agricole à risque et (2) àmettre en évidence et à quantifier l’importance de la dispersion des nématodes à kyste par les travauxdu sol et par le matériel agricole à l’échelle parcellaire et inter-parcellaire. Les travaux menés enpartenariat avec des industriels ainsi qu’en stations expérimentales ont permis la production d’un outilinteractif d’analyse de risque caractérisant les phases critiques de différents process de traitements desdéchets et d’effluents. Il apporte des éléments de réponse sur les performances d’assainissement dedifférents procédés de décontamination comme le compostage, le lagunage, la méthanisation, lachloration et le traitement thermique. Ce projet a aussi permis de cribler, en conditions contrôlées, desespèces végétales non favorables à la multiplication des nématodes à galle M. chitwoodi et M. fallax etpouvant être proposées comme plantes de rupture sur des sites contaminés.

Published
2020

25. Identification of plant genes regulated in resistant potato Solanum sparsipilum during the early stages of infection by Globodera pallida

Author

Jolivet, Katell, Grenier, Eric, Bouchet, Jean-Paul, Esquibet, Magali, Kerlan, Marie-Claire, Caromel, Bernard, Mugniery, Didier, and Lefebvre, Veronique

Subjects
Potatoes -- Genetic aspects, Potatoes -- Physiological aspects, Plant defenses -- Genetic aspects, Plant nematodes -- Chemical properties, Plant nematodes -- Genetic aspects, Genes -- Identification and classification, Biological sciences
Abstract

Abstract: Using a complementary (c)DNA-amplified fragment length polymorphism (AFLP) approach, we investigated differential gene expression linked to resistance mechanisms during the incompatible potato--Globodera pallida interaction. Expression was compared between a [...]

Published
2007

29. Molecular approaches to the taxonomic position of Peruvian potato cyst nematodes and gene pool similarities in indigenous and imported populations of Globodera

Author

Grenier, Eric, Bossis, Michel, Fouville, Didier, Renault, Lionel, and Mugniery, Didier

Subjects
Andes -- Environmental aspects, Europe -- Environmental aspects, Heredity -- Research, Genetic research -- Reports, Pests -- Genetic aspects, Nematoda -- Genetic aspects, Population genetics -- Research, Cladistic analysis -- Research, Biological sciences
Abstract

Molecular approaches have been used to study taxonomy of Peruvian potato cyst nematodes and gene pool similarities in populations of Globodera, both imported and native. It was a surprise to see that although random amplified polymorphic DNA (RAPD) showed a higher variability in the Peruvian group than in the European one, some characteristic proteins were found using 2-DGE in some European populations although it was not possible to find any in the Peruvian ones.

Published
2001

31. The Military Orthopedics Tracking Injuries and Outcomes Network: A Solution for Improving Musculoskeletal Care in the Military Health System

Author

Mauntel, Timothy C, primary, Tenan, Matthew S, additional, Freedman, Brett A, additional, Potter, Benjamin K, additional, Provencher, Matthew T, additional, Tokish, John M, additional, Lee, Ian E, additional, Rhon, Daniel I, additional, Bailey, James R, additional, Burns, Travis C, additional, Cameron, Kenneth L, additional, Grenier, Eric S, additional, Haley, Chad A, additional, Leclere, Lance E, additional, McDonald, Lucas S, additional, Owens, Brett D, additional, Pallis, Mark P, additional, Posner, Matthew A, additional, Rivera, Jessica C, additional, Roach, Christopher J, additional, Robins, Richard J, additional, Schmitz, Matthew R, additional, Sheean, Andrew J, additional, Slabaugh, Mark A, additional, Volk, William R, additional, and Dickens, Jonathan F, additional

Published
2020
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38. A High-Sensitivity International Knee Documentation Committee Survey Index From the PROMIS System: The Next-Generation Patient-Reported Outcome for a Knee Injury Population.

Author

Tenan, Matthew S., Robins, Richard J., Sheean, Andrew J., Dekker, Travis J., Bailey, James R., Bharmal, Husain M., Bradley, Matthew W., Cameron, Kenneth L., Burns, Travis C., Freedman, Brett A., Galvin, Joseph W., Grenier, Eric S., Haley, Chad A., Hurvitz, Andrew P., LeClere, Lance E., Lee, Ian, Mauntel, Timothy, McDonald, Lucas S., Nesti, Leon J., and Owens, Brett D.

Subjects
CONFIDENCE intervals, HEALTH outcome assessment, SURVEYS, DESCRIPTIVE statistics, RECEIVER operating characteristic curves, KNEE injuries, LONGITUDINAL method, ALGORITHMS, KNEE surgery
Abstract

Background: Patient-reported outcomes (PROs) measure progression and quality of care. While legacy PROs such as the International Knee Documentation Committee (IKDC) survey are well-validated, a lengthy PRO creates a time burden on patients, decreasing adherence. In recent years, PROs such as the Patient-Reported Outcomes Measurement Information System (PROMIS) Physical Function and Pain Interference surveys were developed as computer adaptive tests, reducing time to completion. Previous studies have examined correlation between legacy PROs and PROMIS; however, no studies have developed effective prediction models utilizing PROMIS to create an IKDC index. While the IKDC is the standard knee PRO, computer adaptive PROs offer numerous practical advantages. Purpose: To develop a nonlinear predictive model utilizing PROMIS Physical Function and Pain Interference to estimate IKDC survey scores and examine algorithm sensitivity and validity. Study Design: Cohort study (diagnosis); Level of evidence, 3. Methods: The MOTION (Military Orthopaedics Tracking Injuries and Outcomes Network) database is a prospectively collected repository of PROs and intraoperative variables. Patients undergoing knee surgery completed the IKDC and PROMIS surveys at varying time points. Nonlinear multivariable predictive models using Gaussian and beta distributions were created to establish an IKDC index score, which was then validated using leave-one-out techniques and minimal clinically important difference analysis. Results: A total of 1011 patients completed the IKDC and PROMIS Physical Function and Pain Interference, providing 1618 complete observations. The algorithms for the Gaussian and beta distribution were validated to predict the IKDC (Pearson = 0.84-0.86; R 2 = 0.71-0.74; root mean square error = 9.3-10.0). Conclusion: The publicly available predictive models can approximate the IKDC score. The results can be used to compare PROMIS Physical Function and Pain Interference against historical IKDC scores by creating an IKDC index score. Serial use of the IKDC index allows for a lower minimal clinically important difference than the conventional IKDC. PROMIS can be substituted to reduce patient burden, increase completion rates, and produce orthopaedic-specific survey analogs. [ABSTRACT FROM AUTHOR]

Published
2021
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40. The Military Orthopedics Tracking Injuries and Outcomes Network: A Solution for Improving Musculoskeletal Care in the Military Health System

Author

Mauntel, Timothy C, Tenan, Matthew S, Freedman, Brett A, Potter, Benjamin K, Provencher, Matthew T, Tokish, John M, Lee, Ian E, Rhon, Daniel I, Bailey, James R, Burns, Travis C, Cameron, Kenneth L, Grenier, Eric S, Haley, Chad A, Leclere, Lance E, McDonald, Lucas S, Owens, Brett D, Pallis, Mark P, Posner, Matthew A, Rivera, Jessica C, Roach, Christopher J, Robins, Richard J, Schmitz, Matthew R, Sheean, Andrew J, Slabaugh, Mark A, Volk, William R, and Dickens, Jonathan F

Published
2022
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42. Génotypage par séquençage : GBS (Genotyping By Sequencing)

Author

Grenier, Eric, Montarry, Josselin, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

43. Populations of the Beet Cyst Nematode Heterodera schachtii Exhibit Strong Differences in Their Life-History Traits Across Changing Thermal Conditions

Author

Fournet, Sylvain, Pellan, Lucile, Porte, Catherine, Piriou, Christophe, Grenier, Eric, Montarry, Josselin, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST

Subjects
Vegetal Biology, analyse de viabilité de population, specialist/generalist, influence de la température, lcsh:QR1-502, hetérodéra schachtii, adaptation, genetic diversity, Microbiology, survival, hatching, lcsh:Microbiology, diversité génétique, genetic variation, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Biologie végétale, Original Research, nématode à kyste
Abstract

International audience; It is widely accepted that climate has an essential influence on the distribution of species and that temperature is the major abiotic factor that affects their life-history traits. Species with very restricted active dispersal abilities and a wide geographical distribution are thus expected to encompass distinct populations adapted to contrasted local conditions. The beet cyst nematode Heterodera schachtii is a good biological model to study temperature adaptation in populations collected from different environments. Here, we tested the effect of temperature on H. schachtii life-history traits using seven field populations from Morocco, Spain, France, Germany, Austria, Poland and Ukraine. We tested hatching and multiplication rates of each population at different temperatures, as well as hatching rates of each population after storage at different temperatures – simulating survival conditions during the inter-cropping period. Results showed a strong temperature effect on the life-history traits explored. Temperature impact on hatching (at different temperatures and after storage at different temperatures) depended on the origin of populations, separating southern from northern ones. Surprisingly, low temperatures influenced hatching less in southern populations. However, for these populations, a storage period at low temperatures strongly reduce subsequent hatching. Conversely, nematode multiplication was not differentially affected by temperatures, as favorable conditions for the host are also favorable for the parasite. Finally, a significant correlation between the genetic diversity and the level of specialization showed that the less diverse populations were more specialized than the more diverse ones..

Published
2018

45. Fig. S1;Fig. S2;Table S1 from Exploring the causes of small effective population sizes in cyst nematodes using artificial Globodera pallida populations

Author

Montarry, Josselin, Bardou-Valette, Sylvie, Mabon, Romain, Pierre-Loup Jan, Fournet, Sylvain, Grenier, Eric, and Petit, Eric J.

Abstract

Likelihood surfaces showing estimated θ and C values with 95 and 99% confidence envelopes (internal and external envelopes of the highest likelihoods, visualized as grey shades, respectively) for (A) the seven initial G. pallida populations, (B) the 23 final G. pallida populations showing significant heterozygote deficits. θ and C are represented on the x-axis and y-axis, respectively.;Probability of fixation of an allele (P(A)), with an initial frequency of 1%, according to its selection coefficient (s) for a diploid population showing either an effective population size (Ne) of 58 individuals (black line) or a census size (N) of 25,740 individuals (grey line). The dotted lines indicate P(A) according to s for the min Ne (25 individuals) an the max Ne (228 individuals).;Number of cysts from each of the four Peruvian G. pallida populations (P83_otuzco1, P252_cusco3, P286_puno1 and P298_amantani2) used to construct the 24 initial populations (Pi_01 to Pi_24).

Published
2018
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50. The hidden diversity of the potato cyst nematode Globodera pallida in the south of Peru.

Author

Thevenoux, Romain, Folcher, Laurent, Esquibet, Magali, Fouville, Didier, Montarry, Josselin, and Grenier, Eric

Subjects
GOLDEN nematode, GLOBODERA pallida, CYST nematodes, SOYBEAN cyst nematode, NEMATODES, POPULATION differentiation, GENETIC distance
Abstract

Our knowledge of the diversity of potato cyst nematodes in their native areas still remains patchy and should be improved. A previous study based on 42 Peruvian Globodera pallida populations revealed a clear south to north phylogeographic pattern, with five well‐supported clades and maximum diversity observed in the south of Peru. In order to investigate this phylogeographic pattern more closely, we genotyped a larger collection of Peruvian populations using both cathepsin L gene sequence data and a new set of 13 microsatellite loci. Using different genetic analyses (STRUCTURE, DAPC), we consistently obtained the same results that led to similar conclusions: the presence of a larger genetic diversity than previously known suggesting the presence of cryptic species in the south of Peru. These investigations also allowed us to clarify the geographic borders of the previously described G. pallida genetic clades and to update our knowledge of the genetic structure of this species in its native area, with the presence of additional clades. A distance‐based redundancy analysis (dbRDA) was also carried to understand whether there was a correlation between the population genetic differentiation and environmental conditions. This analysis showed that genetic distances observed between G. pallida populations are explained firstly by geographic distances, but also by climatic and soil conditions. This work could lead to a revision of the taxonomy that may have strong implications for risk assessment and management, especially on a quarantine species. [ABSTRACT FROM AUTHOR]

Published
2020
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