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181 results on '"Grego, Kathleen Fernandes"'

1. Understanding the effects of Bothrops erythromelas and Bothrops leucurus venoms on human blood coagulation

Author

Venancio de Lima, Eduardo Oliveira, Tasima, Lídia Jorge, Hatakeyama, Daniela Miki, Farias Rodrigues, Maria Amélia, Serino-Silva, Caroline, Stuginski, Daniel Rodrigues, Machado da Silveira, Giovanni Perez, Prezoto, Benedito Carlos, Grego, Kathleen Fernandes, Sant’Anna, Sávio Stefanini, Tanaka-Azevedo, Anita Mitico, and de Morais-Zani, Karen

Published
2025
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4. Seasonality in Crotalus durissus venom

Author

Tasima, Lidia Jorge, Lima, Eduardo Oliveira Venâncio de, Hatakeyama, Daniela Miki, Vidueiros, Jarbas Prado, Stuginski, Daniel Rodrigues, Grego, Kathleen Fernandes, and Tanaka-Azevedo, Anita Mitico

Published
2024
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7. Snake venom color and L-amino acid oxidase: An evidence of long-term captive Crotalus durissus terrificus venom plasticity

Author

Lima, Eduardo Oliveira Venancio de, Tasima, Lídia Jorge, Hatakeyama, Daniela Miki, Serino-Silva, Caroline, Rodrigues, Caroline Fabri Bittencourt, Galizio, Nathália da Costa, Chiarelli, Tassia, Nishiduka, Erika Sayuri, Rocha, Marisa Maria Teixeira da, Sant’Anna, Sávio Stefanini, Grego, Kathleen Fernandes, Tashima, Alexandre Keiji, Tanaka-Azevedo, Anita Mitico, and Morais-Zani, Karen de

Published
2021
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11. Proteomics and life-history variability of Endogenous Phospholipases A2 Inhibitors (PLIs) in Bothrops jararaca plasma

Author

Serino-Silva, Caroline, primary, Bittencourt Rodrigues, Caroline Fabri, additional, Miyamoto, Jackson Gabriel, additional, Hatakeyama, Daniela Miki, additional, Kavazoi, Victor Koiti, additional, Da Rocha, Marisa Maria Teixeira, additional, Tanaka, Aparecida Sadae, additional, Tashima, Alexandre Keiji, additional, de Morais-Zani, Karen, additional, Grego, Kathleen Fernandes, additional, and Tanaka-Azevedo, Anita Mitico, additional

Published
2024
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18. Antileishmanial effects of γCdcPLI, a phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum, on Leishmania (Leishmania) amazonensis

Author

Gonçalves, Marina Neves, primary, Lopes, Daiana Silva, additional, Teixeira, Samuel Cota, additional, Teixeira, Thaise Lara, additional, de Freitas, Vitor, additional, Costa, Tássia Rafaella, additional, Gimenes, Sarah Natalie Cirilo, additional, de Camargo, Isabella Mitie, additional, de Souza, Guilherme, additional, da Silva, Marcelo Santos, additional, Azevedo, Fernanda Van Petten de Vasconcelos, additional, Grego, Kathleen Fernandes, additional, Santos, Luísa Carregosa, additional, Oliveira, Vinícius Queiroz, additional, da Silva, Claudio Vieira, additional, Rodrigues, Renata Santos, additional, Yoneyama, Kelly Aparecida Geraldo, additional, Clissa, Patricia Bianca, additional, and Rodrigues, Veridiana de Melo, additional

Published
2023
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25. In depth transcriptome unravels the biotechnological arsenal of toxins and viral sequences in the Bothrops jararaca venom gland

Author

Pereira, Leandro De Mattos, Messias, Elisa Alves, Sorroche, Bruna Pereira, Oliveira, Angela Das Neves, Arantes, Lidia Maria Rebolho Batista, Carvalho, Ana Carolina De, Tanaka-Azevedo, Anita Mitico, Grego, Kathleen Fernandes, Carvalho, André Lopes, and Melendez, Matias Eliseo

Subjects
Venom Gland, transcriptomes
Abstract

Background: Lack of complete genomic data of Bothrops jararaca impedes molecular biology research focusing on biotechnological applications of venom gland components. Identification of full-length coding regions of genes is crucial for the correct molecular cloning design. Methods: RNA was extracted from the venom gland of one adult female specimen of Bothrops jararaca. Deep-sequencing of the mRNA library was performed using Illumina NextSeq 500 platform. De novo assembly of the B. jararaca transcriptome was done using Trinity. Annotation was performed using Blast2GO. All predicted proteins after clustering step were blasted against Non-redundant protein database of NCBI using BLASTP. Metabolic pathways present in the transcriptome were annotated using the KAAS - KEGG Automatic Annotation Server. Toxins were identified in the B. jararaca predicted proteome using BLASTP, against all protein sequences obtained from Animal toxin annotation project from Uniprot KB/Swiss-Prot database. Figures and data visualization were performed using ggplot packages in R language environment. Results: We described the in deep transcriptome analysis of B. jararaca venom gland and novo assembled 76,765 isoforms and 96,044 transcribed genes and were predicted 41,196 unique proteins, from which 33,034 were annotated. The most abundant transcripts was the Zinc metalloproteinase-disintegrin-like jararhagin. Moreover, we identified 78 distinct functional classes of proteins, including toxins, inhibitors and tumor suppressors. Other venom proteins identified were the hemolytic lethal factors stonustoxin and verrucotoxin, venom allergen 3-like, reversion-inducing cysteine-rich protein with Kazal motifs, relA-associated inhibitor, MASP1 and MASP2, translationally-controlled tumor, inhibitors of phospholipase A2, ribonuclease inhibitor, protease and metalloproteinases. We also identified sequences of transposons, retrotransposons, retrovirus, including retroviral envelope protein syncytin. Conclusion: We conclude that the application of deep sequencing to the analysis of snake venom transcriptomes, may represent invaluable insight on therapeutic peptides or proteins for different illnesses., This study was supported by the Barretos Cancer Hospital (PAIP – Research Support Incentive Program)., {"references":["https://doi.org/10.1590/1678-9199-jvatitd-2019-0058"]}

Published
2020
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26. Maintenance of venomous snakes in captivity for venom production at Butantan Institute from 1908 to the present: a scoping history

Author

Grego, Kathleen Fernandes, primary, Vieira, Samira Emanuela Maria, additional, Vidueiros, Jarbas Prado, additional, Serapicos, Eliana de Oliveira, additional, Barbarini, Cibele Cíntia, additional, Silveira, Giovanni Perez Machado da, additional, Rodrigues, Fabíola de Souza, additional, Alves, Lucas de Carvalho Francisco, additional, Stuginski, Daniel Rodrigues, additional, Rameh-de-Albuquerque, Luciana Carla, additional, Furtado, Maria de Fátima Domingues, additional, Tanaka-Azevedo, Anita Mitico, additional, Morais-Zani, Karen de, additional, Rocha, Marisa Maria Teixeira da, additional, Fernandes, Wilson, additional, and Sant’Anna, Sávio Stefanini, additional

Published
2021
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30. B. neuwiedi complex: What the venom of these snake species have in common? Compositional and functional investigation of venoms from Bothrops neuwiedi complex

Author

da Costa Galizio, Nathália, primary, da Silva Aguiar, Weslei, additional, Serino-Silva, Caroline, additional, Rodrigues, Caroline Fabri Bittencourt, additional, Stuginski, Daniel Rodrigues, additional, da Rocha, Marisa Maria Teixeira, additional, de Oliveira, Roberto Baptista, additional, Sant’Anna, Sávio Stefanini, additional, Grego, Kathleen Fernandes, additional, Tanaka-Azevedo, Anita Mitico, additional, and de Morais-Zani, Karen, additional

Published
2020
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31. Are you what you eat? The influence of diet on compositional and functional activities of Bothrops moojeni snakes submitted to diet shift

Author

Galizio, Nathalia, primary, Aguiar, Weslei, additional, Chiarelli, Tassia, additional, Serino-Silva, Caroline, additional, Rodrigues, Caroline Fabri Bittencourt, additional, Hatakeyama, Daniela Miki, additional, de Carvalho Francisco Alves, Lucas, additional, Tashima, Alexandre Keiji, additional, Sant'Anna, Sávio Stefanini, additional, Grego, Kathleen Fernandes, additional, Tanaka-Azevedo, Anita Mitico, additional, and de Morais-Zani, Karen, additional

Published
2020
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32. Influence of size, sex and age on venom yield of Bothrops leucurus (Serpentes, Viperidae) under captivity conditions.

Author

Machado Braga, Jacqueline Ramos, Cabus Oitaven, Leonardo Pessoa, Maria Teixeira da Rocha, Marisa, Maria Vieira, Samira Emanuela, Terenciano Rúbio, Diego, Stefanini Sant'anna, Sávio, and Grego, Kathleen Fernandes

Subjects
SNAKE venom, VENOM, VIPERIDAE, BOTHROPS, SNAKES, ANTIVENINS, AGE groups, CAPTIVITY, COLUBRIDAE
Abstract

Snake venom is an expensive metabolic weapon used for digestion and defense. Detailed studies on the production of venoms are important for the manufacture of antivenoms and for the therapeutic management of snakebites. Bothrops leucurus is one of the snakes of medical importance responsible for a large number of accidents in Northeast Brazil. To establish the correlation between Bothrops leucurus venom (Blv) yield, under captive conditions, and the morphological characteristics (body mass and length), sex and age, 31 specimens were milked during one year, grouped by sex and age (juvenile, adult and long-lived), totaling 106 extractions in that period. We evaluated the electrophoretic profile (SDS-PAGE) under reducing conditions, the 50% lethal dose (LD50) and the minimum coagulant dose (DMC) of the extracted venoms. The body size was positively correlated with venom production in B. leucurus snakes. Regardless of sex and age, the venom showed no differences between liquid and solid composition or between right and left fang, however, the production of venom in females was twice the one found in males and more lethal. The clotting ability was lost as the animals aged, indicating that older snakes are not the best choice for venom pools in the production of antivenoms. These results are important for the choice of animals to antivenom production, and to understand the biological effects of snake venoms under captive conditions. [ABSTRACT FROM AUTHOR]

Published
2022
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33. BoaγPLI from Boa constrictor Blood is a Broad-Spectrum Inhibitor of Venom PLA2 Pathophysiological Actions.

Author

Rodrigues, Caroline Fabri Bittencourt, Zdenek, Christina N., Serino-Silva, Caroline, de Morais-Zani, Karen, Grego, Kathleen Fernandes, Bénard-Valle, Melisa, Neri-Castro, Edgar, Alagón, Alejandro, Tanaka-Azevedo, Anita Mitico, and Fry, Bryan Grieg

Subjects
SNAKE venom, VENOM, POISONOUS snakes, PREDATION, TOXINS
Abstract

The use of venom in predation exerts a corresponding selection pressure for the evolution of venom resistance. One of the mechanisms related to venom resistance in animals (predators or prey of snakes) is the presence of molecules in the blood that can bind venom toxins, and inhibit their pharmacological effects. One such toxin type are venom phospholipase A2s (PLA2s), which have diverse effects including anticoagulant, myotoxic, and neurotoxic activities. BoaγPLI isolated from the blood of Boa constrictor has been previously shown to inhibit venom PLA2s that induced myotoxic and edematogenic activities. Recently, in addition to its previously described and very potent neurotoxic effect, the venoms of American coral snakes (Micrurus species) have been shown to have anticoagulant activity via PLA2 toxins. As coral snakes eat other snakes as a major part of their diet, neonate Boas could be susceptible to predation by this sympatric species. Thus, this work aimed to ascertain if BoaγPLI provided a protective effect against the anticoagulant toxicity of venom from the model species Micrurus laticollaris in addition to its ability shown previously against other toxin types. Using a STA R Max coagulation analyser robot to measure the effect upon clotting time, and TEG5000 thromboelastographers to measure the effect upon clot strength, we evaluated the ability of BoaγPLI to inhibit M. laticollaris venom. Our results indicate that BoaγPLI is efficient at inhibiting the M. laticollaris anticoagulant effect, reducing the time of coagulation (restoring them closer to non-venom control values) and increasing the clot strength (restoring them closer to non-venom control values). These findings demonstrate that endogenous PLA2 inhibitors in the blood of non-venomous snakes are multi-functional and provide broad resistance against a myriad of venom PLA2-driven toxic effects including coagulotoxicity, myotoxicity, and neurotoxicity. This novel form of resistance could be evidence of selective pressures caused by predation from venomous snakes and stresses the need for field-based research aimed to expand our understanding of the evolutionary dynamics of such chemical arms race. [ABSTRACT FROM AUTHOR]

Published
2021
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34. BoaγPLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor

Author

Rodrigues, Caroline Fabri Bittencourt, primary, Serino-Silva, Caroline, additional, Morais-Zani, Karen de, additional, Kavazoi, Victor Koiti, additional, Carvalho, Marcelo Pires Nogueira, additional, Grego, Kathleen Fernandes, additional, Chiarelli, Tassia, additional, Tashima, Alexandre Keiji, additional, Toyama, Marcos Hikari, additional, and Tanaka-Azevedo, Anita Mitico, additional

Published
2020
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35. In-depth transcriptome reveals the potential biotechnological application of Bothrops jararaca venom gland

Author

Pereira, Leandro de Mattos, primary, Messias, Elisa Alves, additional, Sorroche, Bruna Pereira, additional, Oliveira, Angela das Neves, additional, Arantes, Lidia Maria Rebolho Batista, additional, Carvalho, Ana Carolina de, additional, Tanaka-Azevedo, Anita Mitico, additional, Grego, Kathleen Fernandes, additional, Carvalho, André Lopes, additional, and Melendez, Matias Eliseo, additional

Published
2020
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37. Geographic variation of individual venom profile of Crotalus durissus snakes

Author

Silva-Júnior, Leandro Norberto da, primary, Abreu, Lara de Souza, additional, Rodrigues, Caroline Fabri Bittencourt, additional, Galizio, Nathália da Costa, additional, Aguiar, Weslei da Silva, additional, Serino-Silva, Caroline, additional, Santos, Valdomiro Souza dos, additional, Costa, Isabella Alves, additional, Oliveira, Luis Vicente Franco, additional, Sant’Anna, Sávio Stefanini, additional, Grego, Kathleen Fernandes, additional, Tanaka-Azevedo, Anita Mitico, additional, Rodrigues, Leandro Nascimento da Silva, additional, and Morais-Zani, Karen de, additional

Published
2020
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38. Venom complexity of Bothrops atrox (common lancehead) siblings

Author

Hatakeyama, Daniela Miki, primary, Tasima, Lídia Jorge, additional, Bravo-Tobar, Cesar Adolfo, additional, Serino-Silva, Caroline, additional, Tashima, Alexandre Keiji, additional, Rodrigues, Caroline Fabri Bittencourt, additional, Aguiar, Weslei da Silva, additional, Galizio, Nathália da Costa, additional, Lima, Eduardo Oliveira Venancio de, additional, Kavazoi, Victor Koiti, additional, Gutierrez-Marín, Juan David, additional, Farias, Iasmim Baptista de, additional, Sant’Anna, Sávio Stefanini, additional, Grego, Kathleen Fernandes, additional, Morais-Zani, Karen de, additional, and Tanaka-Azevedo, Anita Mitico, additional

Published
2020
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39. Are you what you eat? An investigation correlates diet and snake venom composition and function in Bothrops species

Author

Da Costa Galizio, Nathália, primary, Serino-Silva, Caroline, additional, Da Silva Aguiar, Weslei, additional, Hatakeyama, Daniela Miki, additional, De Carvalho Francisco Alves, Lucas, additional, Sant'anna, Sávio Stefanini, additional, Grego, Kathleen Fernandes, additional, Tanaka-Azevedo, Anita Mitico, additional, and De Morais-Zani, Karen, additional

Published
2019
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41. B. neuwiedi complex: what the venom of these six snake species have in common?

Author

Da Costa Galizio, Nathália, primary, Da Silva Aguiar, Weslei, additional, Serino-Silva, Caroline, additional, Stuginski, Daniel Rodrigues, additional, Teixeira Da Rocha, Marisa Maria, additional, De Oliveira, Roberto Baptista, additional, Sant'anna, Sávio Stefanini, additional, Grego, Kathleen Fernandes, additional, Tanaka-Azevedo, Anita Mitico, additional, and De Morais-Zani, Karen, additional

Published
2019
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42. Structural and functional characterization of the phospholipase A2 plasma inhibitor (PLI) from Boa constrictor

Author

Bittencourt Rodrigues, Caroline Fabri, primary, Silva, Caroline Serino, additional, De Morais Zani, Karen, additional, Kavazoi, Victor Koiti, additional, Grego, Kathleen Fernandes, additional, Nogueira Carvalho, Marcelo Pires, additional, Tashima, Alexandre Keiji, additional, Toyama, Marcos Hikari, additional, and Tanaka Azevedo, Anita Mitico, additional

Published
2019
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43. Brazilian Crotalic Reference Venom: Can we include the captive snake venom?

Author

Tasima, Lídia Jorge, primary, De Morais Zani, Karen, additional, Hatakeyama, Daniela Miki, additional, Venancio De Lima, Eduardo Oliveira, additional, Bittencourt Rodrigues, Caroline Fabri, additional, Silva, Caroline Serino, additional, Sant'anna, Sávio Stefanini, additional, Grego, Kathleen Fernandes, additional, and Tanaka Azevedo, Anita Mitico, additional

Published
2019
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44. Does captivity influence the presence of LAAO in the venom of Crotalus durissus terrificus?

Author

Venancio De Lima, Eduardo Oliveira, primary, Tasima, Lídia Jorge, additional, Hatakeyama, Daniela Miki, additional, Da Costa Galizio, Nathália, additional, Silva, Caroline Serino, additional, Sant'anna, Sávio Stefanini, additional, Grego, Kathleen Fernandes, additional, Tanaka Azevedo, Anita Mitico, additional, and De Morais Zani, Karen, additional

Published
2019
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48. Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations

Author

Aguiar, Weslei da Silva, primary, Galizio, Nathália da Costa, additional, Serino-Silva, Caroline, additional, Sant’Anna, Sávio Stefanini, additional, Grego, Kathleen Fernandes, additional, Tashima, Alexandre Keiji, additional, Nishiduka, Erika Sayuri, additional, Morais-Zani, Karen de, additional, and Tanaka-Azevedo, Anita Mitico, additional

Published
2019
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50. Comparative study of protein profile and enzymatic activities of venoms from snake species that compose the Bothrops neuwiedi complex

Author

da Costa Galizio, Nathália, primary, Silva, Caroline Serino, additional, Stuginski, Daniel Rodrigues, additional, Teixeira da Rocha, Marisa Maria, additional, Baptista de Oliveira, Roberto, additional, Sant'Anna, Sávio Stefanini, additional, Grego, Kathleen Fernandes, additional, Tanaka-Azevedo, Anita Mitico, additional, and de Morais-Zani, Karen, additional

Published
2019
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