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Your search keyword '"Grdzelishvili, Nino"' showing total 13 results
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13 results on '"Grdzelishvili, Nino"'

2. Characterization of a Bacteriophage GEC_vB_Bfr_UZM3 Active against Bacteroides fragilis

Author

Bakuradze, Nata, primary, Merabishvili, Maia, additional, Kusradze, Ia, additional, Ceyssens, Pieter-Jan, additional, Onsea, Jolien, additional, Metsemakers, Willem-Jan, additional, Grdzelishvili, Nino, additional, Natroshvili, Guliko, additional, Tatrishvili, Tamar, additional, Lazvliashvili, Davit, additional, Mitskevich, Nunu, additional, Pirnay, Jean-Paul, additional, and Chanishvili, Nina, additional

Published
2023
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3. Development of an in vitro homeostasis model between airway epithelial cells, bacteria and bacteriophages: a time-lapsed observation of cell viability and inflammatory response

Author

Tzani-Tzanopoulou, Panagiota, primary, Rozumbetov, Ramazan, additional, Taka, Styliani, additional, Doudoulakakis, Anastassios, additional, Lebessi, Evangelia, additional, Chanishvili, Nina, additional, Kakabadze, Elene, additional, Bakuradze, Nata, additional, Grdzelishvili, Nino, additional, Goderdzishvili, Marina, additional, Legaki, Evangelia, additional, Andreakos, Evangelos, additional, Papadaki, Maria, additional, Megremis, Spyridon, additional, Xepapadaki, Paraskevi, additional, Kaltsas, Grigoris, additional, Akdis, Cezmi A., additional, and Papadopoulos, Nikolaos G., additional

Published
2022
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4. Gut health of children with autism spectrum disorder

Author

Kakabadze, Elene, primary, Makalatia, Khatuna, additional, Bakuradze, Nata, additional, Grdzelishvili, Nino, additional, Kereselidze, Sophie, additional, Ediberidze, Tamar, additional, Berulava, Veriko, additional, Sabauri, Nana, additional, Cholokashvili, Nana, additional, Javakhishvili, Elene, additional, Natroshvili, Gulnara, additional, Tatrishvili, Tamar, additional, Motsikulashvili, Mariam, additional, Chanishvili, Nina, additional, and Abiatari, Ivane, additional

Published
2022
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6. In Vitro Evaluation of the Therapeutic Potential of Phage VA7 against Enterotoxigenic Bacteroides fragilis Infection

Author

Bakuradze, Nata, primary, Merabishvili, Maya, additional, Makalatia, Khatuna, additional, Kakabadze, Elene, additional, Grdzelishvili, Nino, additional, Wagemans, Jeroen, additional, Lood, Cedric, additional, Chachua, Irakli, additional, Vaneechoutte, Mario, additional, Lavigne, Rob, additional, Pirnay, Jean-Paul, additional, Abiatari, Ivane, additional, and Chanishvili, Nina, additional

Published
2021
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7. Investigation of Salmonella Phage–Bacteria Infection Profiles: Network Structure Reveals a Gradient of Target-Range from Generalist to Specialist Phage Clones in Nested Subsets

Author

Makalatia, Khatuna, primary, Kakabadze, Elene, additional, Bakuradze, Nata, additional, Grdzelishvili, Nino, additional, Stamp, Ben, additional, Herman, Ezra, additional, Tapinos, Avraam, additional, Coffey, Aidan, additional, Lee, David, additional, Papadopoulos, Nikolaos G., additional, Robertson, David L., additional, Chanishvili, Nina, additional, and Megremis, Spyridon, additional

Published
2021
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9. Characterization of Salmonella Isolates from Various Geographical Regions of the Caucasus and Their Susceptibility to Bacteriophages

Author

Makalatia, Khatuna, primary, Kakabadze, Elene, additional, Wagemans, Jeroen, additional, Grdzelishvili, Nino, additional, Bakuradze, Nata, additional, Natroshvili, Gulnara, additional, Macharashvili, Nino, additional, Sedrakyan, Anahit, additional, Arakelova, Karine, additional, Ktsoyan, Zhanna, additional, Zakharyan, Magdalina, additional, Gevorgyan, Zaruhi, additional, Mnatsakanyan, Armine, additional, Tishkova, Farida, additional, Lood, Cédric, additional, Vandenheuvel, Dieter, additional, Lavigne, Rob, additional, Pirnay, Jean-Paul, additional, De Vos, Daniel, additional, Chanishvili, Nina, additional, and Merabishvili, Maia, additional

Published
2020
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10. Automated Phage Isolation

Author

Trojok Rüdiger, Kakabadze Elene, Gaudenz Urs, Sczyrba Thomas, Pfeifer Michael, Jefimowicz Andreas, Cosenza Marco R, Telzerow Anja, Landry Jonathan, Chollet Julian, Grdzelishvili Nino, Makalatia Khatuna, and Chanishvili Nina

Subjects
diybio, biohacking, phage therapy, bioteleportation, bio server, phage isolation, phage trainer
Abstract

Citizens scientists organised in the Do It Yourself Biology (DIYbio) community identified phage therapy as the best solution to the antimicrobial resistance crisis. Since 2014 the community develops open source and low budget liquid handling automation technology such as digital microfluidic chips, peristaltic and syringe pumps and optical and electrical sensors to facilitate phage isolation from water samples. In 2017 a multidrugresistant pathogen contamination was found in an educational reagent kit used by DIYbiologists. The pathogen was sequenced (Whole Genome Sequencing) on an MiSeq Illumina platform and identified as Enterobacter hormaechei subsp. Steigerwaltii. Subsequently five novel phages were isolated from Tbilisi river water for biological decontamination. The contamination case is used as a test run to merge phage therapy protocols with our latest automation technology to advance personalised phage therapy and biological phage decontamination.

Published
2018
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11. Selection of Potential Therapeutic Bacteriophages that Lyse a CTX-M-15 Extended Spectrum β-Lactamase Producing Salmonella enterica Serovar Typhi Strain from the Democratic Republic of the Congo

Author

Kakabadze, Elene, primary, Makalatia, Khatuna, additional, Grdzelishvili, Nino, additional, Bakuradze, Nata, additional, Goderdzishvili, Marina, additional, Kusradze, Ia, additional, Phoba, Marie-France, additional, Lunguya, Octavie, additional, Lood, Cédric, additional, Lavigne, Rob, additional, Jacobs, Jan, additional, Deborggraeve, Stijn, additional, De Block, Tessa, additional, Van Puyvelde, Sandra, additional, Lee, David, additional, Coffey, Aidan, additional, Sedrakyan, Anahit, additional, Soentjens, Patrick, additional, De Vos, Daniel, additional, Pirnay, Jean-Paul, additional, and Chanishvili, Nina, additional

Published
2018
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12. Selection of Potential Therapeutic Bacteriophages that Lyse a CTX-M-15 Extended Spectrum β-Lactamase Producing Salmonellaenterica Serovar Typhi Strain from the Democratic Republic of the Congo.

Author

Kakabadze, Elene, Makalatia, Khatuna, Grdzelishvili, Nino, Bakuradze, Nata, Goderdzishvili, Marina, Kusradze, Ia, Phoba, Marie-France, Lunguya, Octavie, Lood, Cédric, Lavigne, Rob, Jacobs, Jan, Deborggraeve, Stijn, De Block, Tessa, Van Puyvelde, Sandra, Lee, David, Coffey, Aidan, Sedrakyan, Anahit, Soentjens, Patrick, De Vos, Daniel, and Pirnay, Jean-Paul

Subjects
SALMONELLA, BACTERIOPHAGES, FOOD pathogens, BETA lactamases, ENTEROBACTERIACEAE
Abstract

Recently, a Salmonella Typhi isolate producing CTX-M-15 extended spectrum β-lactamase (ESBL) and with decreased ciprofloxacin susceptibility was isolated in the Democratic Republic of the Congo. We have selected bacteriophages that show strong lytic activity against this isolate and have potential for phage-based treatment of S. Typhi, and Salmonella in general. [ABSTRACT FROM AUTHOR]

Published
2018
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13. Relevance of the bacteriophage adherence to mucus model for Pseudomonas aeruginosa phages.

Author

Almeida GMdF, Ravantti J, Grdzelishvili N, Kakabadze E, Bakuradze N, Javakhishvili E, Megremis S, Chanishvili N, Papadopoulos N, and Sundberg L-R

Subjects
Humans, Phage Therapy, Animals, Virus Attachment, Clustered Regularly Interspaced Short Palindromic Repeats, Pseudomonas aeruginosa virology, Pseudomonas aeruginosa physiology, Mucus microbiology, Mucus virology, Pseudomonas Phages physiology, Pseudomonas Phages genetics, Mucins metabolism, Pseudomonas Infections microbiology, Pseudomonas Infections therapy
Abstract

Pseudomonas aeruginosa infections are getting increasingly serious as antimicrobial resistance spreads. Phage therapy may be a solution to the problem, especially if improved by current advances on phage-host studies. As a mucosal pathogen, we hypothesize that P. aeruginosa and its phages are linked to the bacteriophage adherence to mucus (BAM) model. This means that phage-host interactions could be influenced by mucin presence, impacting the success of phage infections on the P. aeruginosa host and consequently leading to the protection of the metazoan host. By using a group of four different phages, we tested three important phenotypes associated with the BAM model: phage binding to mucin, phage growth in mucin-exposed hosts, and the influence of mucin on CRISPR immunity of the bacterium. Three of the tested phages significantly bound to mucin, while two had improved growth rates in mucin-exposed hosts. Improved phage growth was likely the result of phage exploitation of mucin-induced physiological changes in the host. We could not detect CRISPR activity in our system but identified two putative anti-CRISPR proteins coded by the phage. Overall, the differential responses seen for the phages tested show that the same bacterial species can be targeted by mucosal-associated phages or by phages not affected by mucus presence. In conclusion, the BAM model is relevant for phage-bacterium interactions in P. aeruginosa , opening new possibilities to improve phage therapy against this important pathogen by considering mucosal interaction dynamics.IMPORTANCESome bacteriophages are involved in a symbiotic relationship with animals, in which phages held in mucosal surfaces protect them from invading bacteria. Pseudomonas aeruginosa is one of the many bacterial pathogens threatening humankind during the current antimicrobial resistance crisis. Here, we have tested whether P. aeruginosa and its phages are affected by mucosal conditions. We discovered by using a collection of four phages that, indeed, mucosal interaction dynamics can be seen in this model. Three of the tested phages significantly bound to mucin, while two had improved growth rates in mucin-exposed hosts. These results link P. aeruginosa and its phages to the bacteriophage adherence to the mucus model and open opportunities to explore this to improve phage therapy, be it by exploiting the phenotypes detected or by actively selecting mucosal-adapted phages for treatment., Competing Interests: G.M.D.F.A., L.-R.S., and University of Jyväskylä have patented the commercial use of mucin in a patent titled "Improved methods and culture media for production, quantification, and isolation of bacteriophages" (FI20185086, PCT/FI2019/050073).

Published
2024
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