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6. NO-Based Signaling in Plants

Author

Wendehenne, David, Courtois, Cécile, Besson, Angélique, Gravot, Antoine, Buchwalter, Annie, Pugin, Alain, Lamotte, Olivier, Lamattina, Lorenzo, editor, and Polacco, Joseph C., editor

Published
2007
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7. Identification and Quantification of Glucosinolates and Phenolics in a Large Panel of Brassica napus Highlight Valuable Genetic Resources for Chemical Ecology and Breeding

Author

Missinou, Anani Amegan, primary, Ferreira de Carvalho, Julie, additional, Marnet, Nathalie, additional, Delhaye, Thomas, additional, Hamzaoui, Oumayma, additional, Abdel Sayed, David, additional, Guitton, Yann, additional, Lebreton, Lionel, additional, Langrume, Christophe, additional, Laperche, Anne, additional, Delourme, Régine, additional, Manzanares-Dauleux, Maria J., additional, Bouchereau, Alain, additional, and Gravot, Antoine, additional

Published
2022
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18. AtHMA3, a [P.sub.1B]-ATPase allowing Cd/Zn/Co/Pb vacuolar storage in Arabidopsis

Author

Morel, Melanie, Crouzet, Jereme, Gravot, Antoine, Auroy, Pascaline, Leonhardt, Nathalie, Vavasseur, Alain, and Richaud, Pierre

Subjects
Adenosine triphosphatase -- Research, Cobalt -- Research, Arabidopsis thaliana -- Physiological aspects, Arabidopsis thaliana -- Genetic aspects, Vacuoles -- Research, Biological sciences, Science and technology
Published
2009

22. NO-Based Signaling in Plants

Author

Wendehenne, David, primary, Courtois, Cécile, additional, Besson, Angélique, additional, Gravot, Antoine, additional, Buchwalter, Annie, additional, Pugin, Alain, additional, and Lamotte, Olivier, additional

Published
2006
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23. Quantitative resistance to clubroot deconvoluted into QTL-specific metabolic modules

Author

Wagner, Geoffrey, Laperche, Anne, Lariagon, Christine, Marnet, Nathalie, Renault, D, Guitton, Yann, Bouchereau, Alain, Delourme, Régine, Manzanares-Dauleux, Maria, Gravot, Antoine, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)

Subjects
metabolic quantitative trait loci, quantitative resistance, Clubroot, oilseed rape, fungi, Brassica napus, food and beverages, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, metabolic QTL, dwarfing gene bzh, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Plasmodiophora brassicae, Metabolomics, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; Plant disease resistance is often under quantitative genetic control. Thus, in a given interaction, plant cellular responses to infection are influenced by resistance or susceptibility alleles at different loci. In this study, a genetic linkage analysis was used to address the complexity of the metabolic responses of Brassica napus roots to infection by Plasmodiophora brassicae. Metabolome profiling and pathogen quantification in a segregating progeny allowed a comparative mapping of quantitative trait loci (QTLs) involved in resistance and in metabolic adjustments. Distinct metabolic modules were associated with each resistance QTL, suggesting the involvement of different underlying cellular mechanisms. This approach highlighted the possible role of gluconasturtiin and two unknown metabolites in the resistance conferred by two QTLs on chromosomes C03 and C09, respectively. Only two susceptibility biomarkers (glycine and glutathione) were simultaneously linked to the three main resistance QTLs, suggesting the central role of these compounds in the interaction. By contrast, several genotype-specific metabolic responses to infection were genetically unconnected to resistance or susceptibility. Likewise, variations of root sugar profiles, which might have influenced pathogen nutrition, were not found to be related to resistance QTLs. This work illustrates how genetic metabolomics can help to understand plant stress responses and their possible links with disease.

Published
2019
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24. Identification and Quantification of Glucosinolates and Phenolics in a Large Panel of Brassica napusHighlight Valuable Genetic Resources for Chemical Ecology and Breeding

Author

Missinou, Anani Amegan, Ferreira de Carvalho, Julie, Marnet, Nathalie, Delhaye, Thomas, Hamzaoui, Oumayma, Abdel Sayed, David, Guitton, Yann, Lebreton, Lionel, Langrume, Christophe, Laperche, Anne, Delourme, Régine, Manzanares-Dauleux, Maria J., Bouchereau, Alain, and Gravot, Antoine

Abstract

Glucosinolate (GLS) and phenolic contents in Brassicaceaecontribute to biotic and abiotic stress responses. Breeding crop accessions harboring agroecologically relevant metabolic profiles require a characterization of the chemical diversity in Brassicagermplasm. This work investigates the diversity of specialized metabolites in 281 accessions of B. napus. First, an LC-HRMS2-based approach allowed the annotation of 32 phenolics and 36 GLSs, revealing 13 branched and linear alkyl-GLSs and 4 isomers of hydroxyphenylalkyl-GLSs, many of which have been rarely reported in Brassica. Then, quantitative UPLC-UV-MS-based profiling was performed in leaves and roots for the whole panel. This revealed striking variations in the content of 1-methylpropyl-GLS (glucocochlearin) and a large variation of tetra- and penta-glucosyl kaempferol derivatives among accessions. It also highlighted two main chemotypes related to sinapoyl-O-hexoside and kaempferol-O-trihexoside contents. By offering an unprecedented overview of the phytochemical diversity in B. napus, this work provides a useful resource for chemical ecology and breeding.

Published
2022
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25. Nitrogen modulation of Medicago truncatula resistance to Aphanomyces euteiches depends on plant genotype

Author

Thalineau, Elise, Fournier, Carine, Gravot, Antoine, Wendehenne, David, Jeandroz, Sylvain, Truong, Hoai-Nam, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Ministère de l'Enseignement Supérieur et de la Recherche, AgroSup Dijon, PARI8, Conseil Régional de Bourgogne, Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
Nitrates, Genotype, plant defence, Glutamine, fungi, food and beverages, Original Articles, Aphanomyces, nitrogen, Aphanomyces euteiches, Gene Expression Regulation, Plant, nitrate, Medicago truncatula, Amino acids, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Plant Proteins
Abstract

SPEIPMAgrosupINRAUB DOCT; International audience; Nitrogen (N) availability can impact plant resistance to pathogens by the regulation of plant immunity. To better understand the links between N nutrition and plant defence, we analysed the impact of N availability on Medicago truncatula resistance to the root pathogen Aphanomyces euteiches. This oomycete is considered to be the most limiting factor for legume production. Ten plant genotypes were tested in vitro for their resistance to A. euteiches in either complete or nitrate-deficient medium. N deficiency led to enhanced or reduced susceptibility depending on the plant genotype. Focusing on four genotypes displaying contrasting responses, we determined the impact of N deficiency on plant growth and shoot N concentration, and performed expression analyses on N- and defence-related genes, as well as the quantification of soluble phenolics and different amino acids in roots. Our analyses suggest that N modulation of plant resistance is not linked to plant response to N deprivation or to mechanisms previously identified to be involved in plant resistance. Furthermore, our studies highlight a role of glutamine in mediating the susceptibility to A. euteiches in M. truncatula.

Published
2018
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28. Increase of Fungal Pathogenicity and Role of Plant Glutamine in Nitrogen-Induced Susceptibility (NIS) To Rice Blast

Author

Huang, Huichuan, Nguyen Thi Thu, Thuy, He, Xiahong, Gravot, Antoine, Bernillon, Stéphane, Ballini, Elsa, Morel, Jean-Benoit, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Faculty of Agronomy, Hue University of Agriculture and Forestry (HUAF), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Natural Science Foundation of Yunnan Province of China : 2016FD025, Vietnamese government, Chinese government, ANR-SYSTERRA program, MetaboHUB program : ANR-11-INBS-0010, Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, and Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)

Subjects
Vegetal Biology, rice, food and beverages, Plant Science, Magnaporthe oryzae, fertilizer, nitrogen, defense, glutamine, notrogen, pathogenicity, effector, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, protection des cultures, Biologie végétale, Original Research
Abstract

BFP Equipe MétabolismeBGPI : équipe 4; International audience; Modifications in glutamine synthetase OsGS1-2 expression and fungal pathogenicity underlie nitrogen-induced susceptibility to rice blast.Understanding why nitrogen fertilization increase the impact of many plant diseases is of major importance. The interaction between Magnaporthe oryzae and rice was used as a model for analyzing the molecular mechanisms underlying Nitrogen-Induced Susceptibility (NIS). We show that our experimental system in which nitrogen supply strongly affects rice blast susceptibility only slightly affects plant growth. In order to get insights into the mechanisms of NIS, we conducted a dual RNA-seq experiment on rice infected tissues under two nitrogen fertilization regimes. On the one hand, we show that enhanced susceptibility was visible despite an over-induction of defense gene expression by infection under high nitrogen regime. On the other hand, the fungus expressed to high levels effectors and pathogenicity-related genes in plants under high nitrogen regime. We propose that in plants supplied with elevated nitrogen fertilization, the observed enhanced induction of plant defense is over-passed by an increase in the expression of the fungal pathogenicity program, thus leading to enhanced susceptibility. Moreover, some rice genes implicated in nitrogen recycling were highly induced during NIS. We further demonstrate that the OsGS1-2 glutamine synthetase gene enhances plant resistance to M. oryzae and abolishes NIS and pinpoint glutamine as a potential key nutrient during NIS.

Published
2017
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29. Quantitative resistance to clubroot infection mediated by transgenerational epigenetic variation in Arabidopsis

Author

Liégard, Benjamin, primary, Baillet, Victoire, additional, Etcheverry, Mathilde, additional, Joseph, Evens, additional, Lariagon, Christine, additional, Lemoine, Jocelyne, additional, Evrard, Aurélie, additional, Colot, Vincent, additional, Gravot, Antoine, additional, Manzanares‐Dauleux, Maria J., additional, and Jubault, Mélanie, additional

Published
2018
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30. Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot

Author

Gravot, Antoine, Richard, Gautier, Lime, Tanguy, Lariagon, Christine, Lemoine, Jocelyne, Vicente, Jorge, Robert-Seilaniantz, Alexandre, Holdsworth, Michael J., and Manzanares-Dauleux, Maria J.

Subjects
food and beverages, Ethanol fermentation, Plant gall disease, Clubroot, Plasmodiophora, Arabidopsis, ADH1, PDC2, N-end rule pathway, Hypoxia, ERFVII
Abstract

BackgroundThe induction of alcohol fermentation in roots is a plant adaptive response to flooding stress and oxygen deprivation. Available transcriptomic data suggest that fermentation-related genes are also frequently induced in roots infected with gall forming pathogens, but the biological significance of this induction is unclear. In this study, we addressed the role of hypoxia responses in Arabidopsis roots during infection by the clubroot agent Plasmodiophora brassicae.ResultsThe hypoxia-related gene markers PYRUVATE DECARBOXYLASE 1 (PDC1), PYRUVATE DECARBOXYLASE 2 (PDC2) and ALCOHOL DEHYDROGENASE 1 (ADH1) were induced during secondary infection by two isolates of P. brassicae, eH and e2. PDC2 was highly induced as soon as 7 days post inoculation (dpi), i.e., before the development of gall symptoms, and GUS staining revealed that ADH1 induction was localised in infected cortical cells of root galls at 21 dpi. Clubroot symptoms were significantly milder in the pdc1 and pdc2 mutants compared with Col-0, but a null T-DNA insertional mutation of ADH1 did not affect clubroot susceptibility. The Arg/N-end rule pathway of ubiquitin-mediated proteolysis controls oxygen sensing in plants. Mutants of components of this pathway, ate1 ate2 and prt6, that both exhibit constitutive hypoxia responses, showed enhanced clubroot symptoms. In contrast, gall development was reduced in quintuple and sextuple mutants where the activity of all oxygen-sensing Group VII Ethylene Response Factor transcription factors (ERFVIIs) is absent (erfVII and prt6 erfVII).ConclusionsOur data demonstrate that the induction of PDC1 and PDC2 during the secondary infection of roots by P. brassicae contributes positively to clubroot development, and that this is controlled by oxygen-sensing through ERFVIIs. The absence of any major role of ADH1 in symptom development may also suggest that PDC activity could contribute to the formation of galls through the activation of a PDH bypass.

Published
2016

31. Additional file 1: of Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot

Author

Gravot, Antoine, Richard, Gautier, Lime, Tanguy, Lemarié, Séverine, Jubault, Mélanie, Lariagon, Christine, Lemoine, Jocelyne, Vicente, Jorge, Robert-Seilaniantz, Alexandre, Holdsworth, Michael, and Manzanares-Dauleux, Maria

Abstract

Methods used for the analysis of the RNAseq dataset E-MTAB-4176 (from reference [19]). (PDF 44 kb)

Published
2016
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32. Additional file 2: of Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot

Author

Gravot, Antoine, Richard, Gautier, Lime, Tanguy, Lemarié, Séverine, Jubault, Mélanie, Lariagon, Christine, Lemoine, Jocelyne, Vicente, Jorge, Robert-Seilaniantz, Alexandre, Holdsworth, Michael, and Manzanares-Dauleux, Maria

Subjects
parasitic diseases, embryonic structures, human activities
Abstract

Effect of filtering expressed genes on genes density. Density plot of log(CPM) when all genes are taken into account (Raw data) and after removing genes with a CPM

Published
2016
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33. Additional file 3: of Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot

Author

Gravot, Antoine, Richard, Gautier, Lime, Tanguy, Lemarié, Séverine, Jubault, Mélanie, Lariagon, Christine, Lemoine, Jocelyne, Vicente, Jorge, Robert-Seilaniantz, Alexandre, Holdsworth, Michael, and Manzanares-Dauleux, Maria

Subjects
parasitic diseases, embryonic structures, human activities
Abstract

Effect of CPM normalization on genes expression profiles. Boxplots representing the expression distribution of the expressed genes (filtered) before and after CPM normalization using TMM method for Normalization Factor calculation. After normalization, the distribution of genes expression of the 24 analyzed samples is similar. (PDF 373 kb)

Published
2016
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34. Additional file 4: of Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot

Author

Gravot, Antoine, Richard, Gautier, Lime, Tanguy, Lemarié, Séverine, Jubault, Mélanie, Lariagon, Christine, Lemoine, Jocelyne, Vicente, Jorge, Robert-Seilaniantz, Alexandre, Holdsworth, Michael, and Manzanares-Dauleux, Maria

Abstract

Assessment of the biological replicates reproducibility. An unsupervised clustering of sample groups has been performed to verify the likeliness of the biological replicates for each condition. Most groups are correlated and no outlier is detected. All replicates have then been kept for differentially expressed genes analysis. (PDF 8 kb)

Published
2016
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36. Two adjacent NLR genes conferring quantitative resistance to clubroot disease in Arabidopsisare regulated by a stably inherited epiallelic variation

Author

Gravot, Antoine, Liégard, Benjamin, Quadrana, Leandro, Veillet, Florian, Aigu, Yoann, Bargain, Tristan, Bénéjam, Juliette, Lariagon, Christine, Lemoine, Jocelyne, Colot, Vincent, Manzanares-Dauleux, Maria J., and Jubault, Mélanie

Abstract

Clubroot caused by the protist Plasmodiophora brassicaeis a major disease affecting cultivated Brassicaceae. Using a combination of quantitative trait locus (QTL) fine mapping, CRISPR-Cas9 validation, and extensive analyses of DNA sequence and methylation patterns, we revealed that the two adjacent neighboring NLR(nucleotide-binding and leucine-rich repeat) genes AT5G47260and AT5G47280cooperate in controlling broad-spectrum quantitative partial resistance to the root pathogen P. brassicaein Arabidopsisand that they are epigenetically regulated. The variation in DNA methylation is not associated with any nucleotide variation or any transposable element presence/absence variants and is stably inherited. Variations in DNA methylation at the Pb-At5.2QTL are widespread across Arabidopsisaccessions and correlate negatively with variations in expression of the two genes. Our study demonstrates that natural, stable, and transgenerationally inherited epigenetic variations can play an important role in shaping resistance to plant pathogens by modulating the expression of immune receptors.

Published
2024
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38. Nitrogen modulation of <italic>Medicago truncatula</italic> resistance to <italic>Aphanomyces euteiches</italic> depends on plant genotype.

Author

Thalineau, Elise, Fournier, Carine, Gravot, Antoine, Wendehenne, David, Jeandroz, Sylvain, and Truong, Hoai‐Nam

Subjects
EFFECT of nitrogen on plants, APHANOMYCES euteiches, MEDICAGO truncatula, PLANT genes, PLANT diseases, PHYTOPATHOGENIC microorganisms
Abstract

Summary: Nitrogen (N) availability can impact plant resistance to pathogens by the regulation of plant immunity. To better understand the links between N nutrition and plant defence, we analysed the impact of N availability on Medicago truncatula resistance to the root pathogen Aphanomyces euteiches. This oomycete is considered to be the most limiting factor for legume production. Ten plant genotypes were tested in vitro for their resistance to A. euteiches in either complete or nitrate‐deficient medium. N deficiency led to enhanced or reduced susceptibility depending on the plant genotype. Focusing on four genotypes displaying contrasting responses, we determined the impact of N deficiency on plant growth and shoot N concentration, and performed expression analyses on N‐ and defence‐related genes, as well as the quantification of soluble phenolics and different amino acids in roots. Our analyses suggest that N modulation of plant resistance is not linked to plant response to N deprivation or to mechanisms previously identified to be involved in plant resistance. Furthermore, our studies highlight a role of glutamine in mediating the susceptibility to A. euteiches in M. truncatula. [ABSTRACT FROM AUTHOR]

Published
2018
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39. Integrative analysis of metabolite and transcript abundance during the short-term response to saline and oxidative stress in the brown alga Ectocarpus siliculosus

Author

Dittami, Simon M., Gravot, Antoine, Renault, David, Goulitquer, Sophie, Eggert, Anja, Bouchereau, Alain, Boyen, Catherine, Tonon, Thierry, Végétaux marins et biomolécules, Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-GOEMAR-Centre National de la Recherche Scientifique (CNRS), Amélioration des Plantes et Biotechnologies Végétales (APBV), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Génétique moléculaire et génétique épidémiologique, Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM), Leibniz Institute for Baltic Sea Research Warnemünde, Rennes Métropole Région Bretagne, European Project, AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
abiotic stress response, osmolytes, g-aminobutyric acid (GABA), urea, amino acid metabolism, proline, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, phaeophyceae, polyunsaturated fatty acids
Abstract

International audience; The model brown alga Ectocarpus siliculosus undergoes extensive transcriptomic changes in response to abiotic stress, many of them related to primary metabolism and particularly to amino acid biosynthesis and degradation. In this study we seek to improve our knowledge of the mechanisms underlying the stress tolerance of this alga, in particular with regard to compatible osmolytes, by examining the effects of these changes on metabolite concentrations. We performed extensive metabolic profiling (urea, amino acids, sugars, polyols, organic acids, fatty acids) of Ectocarpus samples subjected to short-term hyposaline, hypersaline and oxidative stress, and integrated the results with previously published transcriptomic data. The most pronounced changes in metabolite concentrations occurred under hypersaline stress: both mannitol and proline were accumulated, but their low final concentrations indicate that, in this stress condition, both compounds are not likely to significantly contribute to osmoregulation at the level of the entire cell. Urea and trehalose were not detected in any of our samples.We also observed a shift in fatty acid composition from n-3 to n-6 fatty acids under high salinities, and demonstrated the salt stress-induced accumulation of small amounts of g-aminobutyric acid (GABA). GABA could be synthesized in E. siliculosus through a salt stress-induced putrescine-degradation pathway.

Published
2011
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42. Responses to cold and phylogenetic structure in the subterranean crustacean Niphargus rhenorhodanensis

Author

Colson-Proch, Céline, Hervant, Frédéric, Douady, Christophe Jean, Renault, David, Gravot, Antoine, Gibert, Janine, Laboratoire d'Ecologie des Hydrosystèmes Fluviaux (EHF), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Amélioration des Plantes et Biotechnologies Végétales (APBV), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Briand, Valerie

Subjects
[SDE.BE] Environmental Sciences/Biodiversity and Ecology, [SDE.MCG] Environmental Sciences/Global Changes, [SDE.MCG]Environmental Sciences/Global Changes, ComputingMethodologies_GENERAL, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, [SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces, environment, [SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces, environment
Abstract

posterFR2116poster

Published
2007

43. Increase of Fungal Pathogenicity and Role of Plant Glutamine in Nitrogen-Induced Susceptibility (NIS) To Rice Blast.

Author

Huichuan Huang, Thuy Nguyen Thi Thu, Xiahong He, Gravot, Antoine, Bernillon, Stéphane, Ballini, Elsa, and Morel, Jean-Benoit

Subjects
RICE blast disease, NITROGEN fertilizers, RNA sequencing
Abstract

Highlight Modifications in glutamine synthetase OsGS1-2 expression and fungal pathogenicity underlie nitrogen-induced susceptibility to rice blast. Understanding why nitrogen fertilization increase the impact of many plant diseases is of major importance. The interaction between Magnaporthe oryzae and rice was used as a model for analyzing the molecular mechanisms underlying Nitrogen-Induced Susceptibility (NIS). We show that our experimental system in which nitrogen supply strongly affects rice blast susceptibility only slightly affects plant growth. In order to get insights into the mechanisms of NIS, we conducted a dual RNA-seq experiment on rice infected tissues under two nitrogen fertilization regimes. On the one hand, we show that enhanced susceptibility was visible despite an over-induction of defense gene expression by infection under high nitrogen regime. On the other hand, the fungus expressed to high levels effectors and pathogenicity-related genes in plants under high nitrogen regime. We propose that in plants supplied with elevated nitrogen fertilization, the observed enhanced induction of plant defense is over-passed by an increase in the expression of the fungal pathogenicity program, thus leading to enhanced susceptibility. Moreover, some rice genes implicated in nitrogen recycling were highly induced during NIS. We further demonstrate that the OsGS1-2 glutamine synthetase gene enhances plant resistance to M. oryzae and abolishes NIS and pinpoint glutamine as a potential key nutrient during NIS. [ABSTRACT FROM AUTHOR]

Published
2017
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44. The metabolic profiling of osmolyte accumulating transgenics, mutants and extremophile relatives of Arabidopsis to get an insight into drought and salt stress responses in plants

Author

Bouchereau, Alain, Niogret, Marie-Françoise, Deleu, Carole, Leport, Laurent, Le Caherec, Françoise, Gravot, Antoine, Monnier, Chantal, Saligaut, Dany, Larher, François, Interactions cellulaires et moléculaires (ICM), Centre National de la Recherche Scientifique (CNRS)-IFR140-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Amélioration des Plantes et Biotechnologies Végétales (APBV), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2005

47. Metabotyping: A New Approach to Investigate Rapeseed (Brassica napus L.) Genetic Diversity in the Metabolic Response to Clubroot Infection

Author

Wagner, Geoffrey, primary, Charton, Sophie, additional, Lariagon, Christine, additional, Laperche, Anne, additional, Lugan, Raphaël, additional, Hopkins, Julie, additional, Frendo, Pierre, additional, Bouchereau, Alain, additional, Delourme, Régine, additional, Gravot, Antoine, additional, and Manzanares-Dauleux, Maria J., additional

Published
2012
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