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12 results on '"Grasso G.M."'

9. Detection of Cryptosporidium parvum Oocysts in Experimentally Contaminated Lettuce Using Filtration, Immunomagnetic Separation, Light Microscopy, and PCR

Author

Ripabelli, G., Leone, A., Sammarco, M.L., Fanelli, I., Grasso, G.M., and McLauchlin, J.

Abstract

Recovery of Cryptosporidium parvum oocysts in a fecal suspension that experimentally contaminated onto lettuce leaves was investigated. Material recovered from the lettuce samples by washing in detergent solutions were concentrated by filtration using the Envirochek Sampling Capsule. Oocysts were concentrated by immunomagnetic separation (IMS) and detected by microscopy following modified Ziehl-Neelsen (MZN) staining. Cryptosporidal DNA was detected using a nested-PCR assay for amplification of a fragment of the Cryptosporidium oocyst wall protein (COWP) gene, which was applied to DNA extracted from both filtrates, and material recovered from MZN stained smears on glass slides after microscopy. No Cryptosporidium were detected by microscopy or by PCR of un-inoculated lettuce leaves. After IMS, means of 0–6.5% of the total numbers of oocysts inoculated were recovered and detected by microscopy. Detection by PCR was less sensitive than microscopy. There was a strong association between successful PCR amplification, the numbers of oocysts detected by microscopy and the numbers of oocysts in the inoculum. This study confirms that C. parvum oocysts can be recovered from contaminated lettuce using filtration and IMS, and detected by microscopy and PCR. However, further evelopments are required to improve recovery of this parasite.

Published
2004
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10. Immunomagnetic separation and conventional culture procedure for detection of naturally occurring Salmonellain raw pork sausages and chicken meat

Author

Ripabelli, G., Sammarco, M.L., Ruberto, A., Iannitto, G., and Grasso, G.M.

Abstract

The aim of the study was to compare immunomagnetic separation (IMS) and conventional selective enrichment procedures using selenite cystine broth (SC) and Rappaport–Vassiliadis broth (RV) in 137 naturally contaminated food samples (69 raw pork sausages and 68 chicken meat). The utilization of SC or IMS appeared to be the most appropriate enrichment procedure: 15 out of 18 Salmonella‐positive samples (83·3%) were detected by SC and 12 (66·7%) by IMS; RV yielded only seven positive isolations (38·9%). However, RV yielded the highest count of Salmonellacolonies per plate and the lowest interference by competing organisms. IMS could become a reliable alternative to standard enrichment procedures and a combined IMS and selective enrichment broth could increase the chance of Salmonellarecovery.

Published
1997
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12. Hospital discharges-based search of acute flaccid paralysis cases 2007-2016 in Italy and comparison with the National Surveillance System for monitoring the risk of polio reintroduction

Author

Stefanelli, Paola, Bellino, Stefania, Fiore, Stefano, Fontana, Stefano, Amato, Concetta, Buttinelli, Gabriele, Ansaldi, Filippo, Binda, Sandro, Pellegrinelli, Laura, Bonaccorsi, Guglielmo, Lorini, Chiara, Brusaferro, Silvio, Camilloni, Barbara, Capannolo, Benita, Mancini, Cristiana, Carraro, Valter, Castiglia, Paolo, Arghittu, Antonella, D'Errico, Marcello Mario, De Stefano, Carlo, Foca, Alfredo, CinziaGerminario, Larocca, Angela, Giammanco, Giovanni M, De Grazia, Simona, Grasso, Guido Maria, DanielaLombardi, Russo, Francesca, Napoletano, Giuseppina, Zanella, Francesca, Spertini, Silvia, Veronesi, Licia, Affanni, Paola, Triassi, Maria, Pennino, Francesca, Vairo, Francesco, Stefanelli, P., Bellino, S., Fiore, S., Fontana, S., Amato, C., Buttinelli, G., Ansaldi, F., Binda, S., Pellegrinelli, L., Bonaccorsi, G., Lorini, C., Brusaferro, S., Camilloni, B., Capannolo, B., Mancini, C., Carraro, V., Castiglia, P., Arghittu, A., D'Errico, M. M., De Stefano, C., Foca, A., Germinario, C., Larocca, A., Giammanco, G. M., De Grazia, S., Grasso, G. M., Lombardi, D., Russo, F., Napoletano, G., Zanella, F., Spertini, S., Veronesi, L., Affanni, P., Triassi, M., Pennino, F., Vairo, F., Stefanelli P., Bellino S., Fiore S., Fontana S., Amato C., Buttinelli G., Ansaldi F., Binda S., Pellegrinelli L., Bonaccorsi G., Lorini C., Brusaferro S., Camilloni B., Capannolo B., Mancini C., Carraro V., Castiglia P., Arghittu A., D'errico M.M., De Stefano C., Foca A., Germinario C., Larocca A., Giammanco G., De Grazia S., Grasso G.M., Lombardi D., Russo F., Napoletano G., Zanella F., Spertini S., Veronesi L., Affanni P., Triassi M., Pennino F., and Vairo F.

Subjects
Male, Settore MED/07 - Microbiologia E Microbiologia Clinica, Pediatrics, ERADICATION, Acute flaccid paralysi, 0302 clinical medicine, WORLDWIDE, Epidemiology, Paralysis, Medicine, 030212 general & internal medicine, Child, Public, Environmental & Occupational Health, 0303 health sciences, Polio, lcsh:Public aspects of medicine, Hospital Records, Acute flaccid paralysis, Patient Discharge, Poliomyelitis, Italy, Child, Preschool, Population Surveillance, Female, Diagnosis code, medicine.symptom, Hospital discharge record, Life Sciences & Biomedicine, Encephalitis, Research Article, Human, medicine.medical_specialty, Adolescent, Myelitis, 03 medical and health sciences, Poliomyelitis eradication, Hospital discharge records, Hospital Record, Humans, National surveillance system, Preschool, Science & Technology, 030306 microbiology, business.industry, Public Health, Environmental and Occupational Health, Infant, lcsh:RA1-1270, medicine.disease, Paralysi, Poliomyeliti, business
Abstract

Background Acute flaccid paralysis (AFP) surveillance has been adopted globally as a key strategy for monitoring the progress of the polio eradication initiative. Hereby, to evaluate the completeness of the ascertainment of AFP cases in Italy, a hospital-discharges based search was carried out. Methods AFP cases occurring between 2007 and 2016 among children under 15 years of age were searched in the Italian Hospital Discharge Records (HDR) database using specific ICD-9-CM diagnostic codes. AFP cases identified between 2015 and 2016 were then compared with those notified to the National Surveillance System (NSS). Results Over a 10-year period, 4163 hospital discharges with diagnosis of AFP were reported in Italy. Among these, 956 (23.0%) were acute infective polyneuritis, 1803 (43.3%) myopathy, and 1408 (33.8%) encephalitis, myelitis and encephalomyelitis. During the study period, a decreasing trend was observed for all diagnoses and overall the annual incidence rate (IR) declined from 5.5 to 4.5 per 100,000 children. Comparing NSS with HDR data in 2015–2016, we found a remarkable underreporting, being AFP cases from NSS only 14% of those recorded in HDR. In particular, the acute infective polyneuritis cases reported to NSS accounted for 42.6% of those detected in HDR, while only 0.9% of myopathy cases and 13.1% of encephalitis/myelitis/encephalomyelitis cases have been notified to NSS. The highest AFP IRs per 100,000 children calculated on HDR data were identified in Liguria (17.4), Sicily (5.7), and Veneto (5.1) Regions; regarding the AFP notified to the NSS, 11 out of 21 Regions failed to reach the number of expected cases (based on 1/100,000 rate), and the highest discrepancies were observed in the Northern Regions. Overall, the national AFP rate was equal to 0.6, therefore did not reach the target value. Conclusions AFP surveillance data are the final measure of a country’s progress towards polio eradication. The historical data obtained by the HDR have been useful to assess the completeness of the notification data and to identify the Regions with a low AFP ascertainment rate in order to improve the national surveillance system.

Published
2019

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