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1. Goodbye Hartmann trial: a prospective, international, multicenter, observational study on the current use of a surgical procedure developed a century ago

Author: Perrone, G, Giuffrida, M, Abu-Zidan, F, Kruger, V, Livrini, M, Petracca, G, Rossi, G, Tarasconi, A, Tian, B, Bonati, E, Mentz, R, Mazzini, F, Campana, J, Gasser, E, Kafka-Ritsch, R, Felsenreich, D, Dawoud, C, Riss, S, Gomes, C, Gomes, F, Gonzaga, R, Canton, C, Pereira, B, Fraga, G, Zem, L, Cordeiro-Fonseca, V, de Mesquita Tauil, R, Atanasov, B, Belev, N, Kovachev, N, Melendez, L, Dimova, A, Dimov, S, Zelic, Z, Augustin, G, Bogdanic, B, Moric, T, Chouillard, E, Bajul, M, De Simone, B, Panis, Y, Esposito, F, Notarnicola, M, Lauka, L, Fabbri, A, Hentati, H, Fnaiech, I, Aurelien, V, Bougard, M, Roulet, M, Demetrashvili, Z, Pipia, I, Merabishvili, G, Bouliaris, K, Koukoulis, G, Doudakmanis, C, Xenaki, S, Chrysos, E, Kokkinakis, S, Vassiliu, P, Michalopoulos, N, Margaris, I, Kechagias, A, Avgerinos, K, Katunin, J, Lostoridis, E, Nagorni, E, Pujante, A, Mulita, F, Maroulis, I, Vailas, M, Marinis, A, Siannis, I, Bourbouteli, E, Manatakis, D, Tasis, N, Acheimastos, V, Maria, S, Stylianos, K, Kuzeridis, H, Korkolis, D, Fradelos, E, Kavalieratos, G, Petropoulou, T, Polydorou, A, Papacostantinou, I, Triantafyllou, T, Kimpizi, D, Theodorou, D, Toutouzas, K, Chamzin, A, Frountzas, M, Schizas, D, Karavokyros, I, Syllaios, A, Charalabopoulos, A, Boura, M, Baili, E, Ioannidis, O, Loutzidou, L, Anestiadou, E, Tsouknidas, I, Petrakis, G, Polenta, E, Bains, L, Gupta, R, Singh, S, Khanduri, A, Bala, M, Kedar, A, Pisano, M, Podda, M, Pisanu, A, Martines, G, Trigiante, G, Lantone, G, Agrusa, A, Di Buono, G, Buscemi, S, Veroux, M, Gioco, R, Veroux, G, Oragano, L, Zonta, S, Lovisetto, F, Feo, C, Pesce, A, Fabbri, N, Marino, F, Perrone, F, Vincenti, L, Papagni, V, Picciariello, A, Rossi, S, Picardi, B, Del Monte, S, Visconti, D, Osella, G, Petruzzelli, L, Pignata, G, Andreuccetti, J, D'Alessio, R, Buonfantino, M, Guaitoli, E, Spinelli, S, Sampietro, G, Corbellini, C, Lorusso, L, Frontali, A, Pezzoli, I, Bonomi, A, Chierici, A, Cotsoglou, C, Manca, G, Delvecchio, A, Musa, N, Casati, M, Letizia, L, Abate, E, Ercolani, G, D'Acapito, F, Solaini, L, Guercioni, G, Cicconi, S, Sasia, D, Borghi, F, Giraudo, G, Sena, G, Castaldo, P, Cardamone, E, Portale, G, Zuin, M, Spolverato, Y, Esposito, M, Isernia, R, Di Salvo, M, Manunza, R, Esposito, G, Agus, M, Asti, E, Bernardi, D, Tonucci, T, Luppi, D, Casadei, M, Bonilauri, S, Pezzolla, A, Panebianco, A, Laforgia, R, De Luca, M, Zese, M, Parini, D, Jovine, E, De Sario, G, Lombardi, R, Aprea, G, Palomba, G, Capuano, M, Argenio, G, Orio, G, Armellino, M, Troian, M, Guerra, M, Nagliati, C, Biloslavo, A, Germani, P, Aizza, G, Monsellato, I, Chahrour, A, Anania, G, Bombardini, C, Bagolini, F, Sganga, G, Fransvea, P, Bianchi, V, Boati, P, Ferrara, F, Palmieri, F, Cianci, P, Gattulli, D, Restini, E, Cillara, N, Cannavera, A, Nita, G, Sarnari, J, Roscio, F, Clerici, F, Scandroglio, I, Berti, S, Cadeo, A, Filippelli, A, Conti, L, Grassi, C, Cattaneo, G, Pighin, M, Papis, D, Gambino, G, Bertino, V, Schifano, D, Prando, D, Fogato, L, Cavallo, F, Ansaloni, L, Picheo, R, Pontarolo, N, Depalma, N, Spampinato, M, D'Ugo, S, Lepre, L, Capponi, M, Campa, R, Sarro, G, Dinuzzi, V, Olmi, S, Uccelli, M, Ferrari, D, Inama, M, Moretto, G, Fontana, M, Favi, F, Picariello, E, Rampini, A, Barberis, A, Azzinnaro, A, Oliva, A, Totaro, L, Benzoni, I, Ranieri, V, Capolupo, G, Carannante, F, Caricato, M, Ronconi, M, Casiraghi, S, Casole, G, Pantalone, D, Alemanno, G, Scheiterle, M, Ceresoli, M, Cereda, M, Fumagalli, C, Zanzi, F, Bolzon, S, Guerra, E, Lecchi, F, Cellerino, P, Ardito, A, Scaramuzzo, R, Balla, A, Lepiane, P, Tartaglia, N, Ambrosi, A, Pavone, G, Palini, G, Veneroni, S, Garulli, G, Ricci, C, Torre, B, Russo, I, Rottoli, M, Tanzanu, M, Belvedere, A, Milone, M, Manigrasso, M, De Palma, G, Piccoli, M, Pattacini, G, Magnone, S, Bertoli, P, Massucco, P, Palisi, M, Luzzi, A, Fleres, F, Clarizia, G, Spolini, A, Kobe, Y, Toma, T, Shimamura, F, Parker, R, Ranketi, S, Mitei, M, Svagzdys, S, Pauzas, H, Zilinskas, J, Poskus, T, Kryzauskas, M, Jakubauskas, M, Zakaria, A, Zakaria, Z, Wong, M, Jusoh, A, Zakaria, M, Cruz, D, Elizalde, A, Reynaud, A, Hernandez, E, Monroy, J, Hinojosa-Ugarte, D, Quiodettis, M, Du Bois, M, Latorraca, J, Major, P, Pedziwiatr, M, Pisarska-Adamczyk, M, Waledziak, M, Kwiatkowski, A, Czyzykowski, L, da Costa, S, Ferreira, A, Almeida, F, Rocha, R, Carneiro, C, Perez, D, Carvas, J, Rocha, C, Ferreira, C, Marques, R, Fernandes, U, Leao, P, Goulart, A, Pereira, R, Patrocinio, S, de Mendonca, N, Manso, M, Morais, H, Cardoso, P, Calu, V, Miron, A, Toma, E, Gachabayov, M, Abdullaev, A, Litvin, A, Nechay, T, Tyagunov, A, Yuldashev, A, Bradley, A, Wilson, M, Panyko, A, Lateckova, Z, Lacko, V, Lesko, D, Soltes, M, Radonak, J, Turrado-Rodriguez, V, Termes-Serra, R, Morales-Sevillano, X, Lapolla, P, Mingoli, A, Brachini, G, Degiuli, M, Sofia, S, Reddavid, R, de Manzoni Garberini, A, Buffone, A, del Pozo, E, Aparicio-Sanchez, D, Dos Barbeito, S, Estaire-Gomez, M, Viton-Herrero, R, de los Angeles Gil Olarte-Marquez, M, Gil-Martinez, J, Alconchel, F, Nicolas-Lopez, T, Rahy-Martin, A, Pelloni, M, Banolas-Suarez, R, Mendoza-Moreno, F, Nisa, F, Diez-Alonso, M, Rodas, M, Agundez, M, Andres, M, Moreira, C, Perez, A, Ponce, I, Gonzalez-Castillo, A, Membrilla-Fernandez, E, Salvans, S, Serradilla-Martin, M, Pardo, P, Rivera-Alonso, D, Dziakova, J, Huguet, J, Valle, N, Ruiz, E, Valcarcel, C, Moreno, C, Salazar, Y, Garcia, J, Mico, S, Lopez, J, Farre, S, Gomez, M, Petit, N, Titos-Garcia, A, Aranda-Narvaez, J, Romacho-Lopez, L, Sanchez-Guillen, L, Aranaz-Ostariz, V, Bosch-Ramirez, M, Martinez-Perez, A, Martinez-Lopez, E, Sebastian-Tomas, J, Jimenez-Riera, G, Jimenez-Vega, J, Cuellar, J, Campos-Serra, A, Munoz-Campana, A, Gracia-Roman, R, Alegre, J, Pinto, F, O'Sullivan, S, Antona, F, Jimenez, B, Lopez-Sanchez, J, Carmona, Z, Fernandez, R, Sierra, I, de Leon, L, Moreno, V, Iglesias, E, Cumplido, P, Bravo, A, Simo, I, Dominguez, C, Caamano, A, Lozano, R, Martinez, M, Torres, A, de Quiros, J, Pellino, G, Cloquell, M, Moller, E, Jalal-Eldin, S, Abdoun, A, Hamid, H, Lohsiriwat, V, Mongkhonsupphawan, A, Baraket, O, Ayed, K, Abbassi, I, Ali, A, Ammar, H, Kchaou, A, Tlili, A, Zribi, I, Colak, E, Polat, S, Koylu, Z, Guner, A, Usta, M, Reis, M, Mantoglu, B, Gonullu, E, Akin, E, Altintoprak, F, Bayhan, Z, Firat, N, Isik, A, Memis, U, Bayrak, M, Altintas, Y, Kara, Y, Bozkurt, M, Kocatas, A, Das, K, Seker, A, Ozer, N, Atici, S, Tuncer, K, Kaya, T, Ozkan, Z, Ilhan, O, Agackiran, I, Uzunoglu, M, Demirbas, E, Altinel, Y, Meric, S, Hacim, N, Uymaz, D, Omarov, N, Balik, E, Tebala, G, Khalil, H, Rana, M, Khan, M, Florence, C, Swaminathan, C, Leo, C, Liasis, L, Watfah, J, Trostchansky, I, Delgado, E, Pontillo, M, Latifi, R, Coimbra, R, Edwards, S, Lopez, A, Velmahos, G, Dorken, A, Gebran, A, Palmer, A, Oury, J, Bardes, J, Seng, S, Coffua, L, Ratnasekera, A, Egodage, T, Echeverria-Rosario, K, Armento, I, Napolitano, L, Sangji, N, Hemmila, M, Quick, J, Austin, T, Hyman, T, Curtiss, W, Mcclure, A, Cairl, N, Biffl, W, Truong, H, Schaffer, K, Reames, S, Banchini, F, Capelli, P, Coccolini, F, Sartelli, M, Bravi, F, Vallicelli, C, Agnoletti, V, Baiocchi, G, Catena, F, Perrone G., Giuffrida M., Abu-Zidan F., Kruger V. F., Livrini M., Petracca G. L., Rossi G., Tarasconi A., Tian B. W. C. A., Bonati E., Mentz R., Mazzini F. N., Campana J. P., Gasser E., Kafka-Ritsch R., Felsenreich D. M., Dawoud C., Riss S., Gomes C. A., Gomes F. C., Gonzaga R. A. T., Canton C. A. B., Pereira B. M., Fraga G. P., Zem L. G., Cordeiro-Fonseca V., de Mesquita Tauil R., Atanasov B., Belev N., Kovachev N., Melendez L. J. J., Dimova A., Dimov S., Zelic Z., Augustin G., Bogdanic B., Moric T., Chouillard E., Bajul M., De Simone B., Panis Y., Esposito F., Notarnicola M., Lauka L., Fabbri A., Hentati H., Fnaiech I., Aurelien V., Bougard M., Roulet M., Demetrashvili Z., Pipia I., Merabishvili G., Bouliaris K., Koukoulis G., Doudakmanis C., Xenaki S., Chrysos E., Kokkinakis S., Vassiliu P., Michalopoulos N., Margaris I., Kechagias A., Avgerinos K., Katunin J., Lostoridis E., Nagorni E. -A., Pujante A., Mulita F., Maroulis I., Vailas M., Marinis A., Siannis I., Bourbouteli E., Manatakis D. K., Tasis N., Acheimastos V., Maria S., Stylianos K., Kuzeridis H., Korkolis D., Fradelos E., Kavalieratos G., Petropoulou T., Polydorou A., Papacostantinou I., Triantafyllou T., Kimpizi D., Theodorou D., Toutouzas K., Chamzin A., Frountzas M., Schizas D., Karavokyros I., Syllaios A., Charalabopoulos A., Boura M., Baili E., Ioannidis O., Loutzidou L., Anestiadou E., Tsouknidas I., Petrakis G., Polenta E., Bains L., Gupta R., Singh S. K., Khanduri A., Bala M., Kedar A., Pisano M., Podda M., Pisanu A., Martines G., Trigiante G., Lantone G., Agrusa A., Di Buono G., Buscemi S., Veroux M., Gioco R., Veroux G., Oragano L., Zonta S., Lovisetto F., Feo C. V., Pesce A., Fabbri N., Marino F., Perrone F., Vincenti L., Papagni V., Picciariello A., Rossi S., Picardi B., Del Monte S. R., Visconti D., Osella G., Petruzzelli L., Pignata G., Andreuccetti J., D'Alessio R., Buonfantino M., Guaitoli E., Spinelli S., Sampietro G. M., Corbellini C., Lorusso L., Frontali A., Pezzoli I., Bonomi A., Chierici A., Cotsoglou C., Manca G., Delvecchio A., Musa N., Casati M., Letizia L., Abate E., Ercolani G., D'Acapito F., Solaini L., Guercioni G., Cicconi S., Sasia D., Borghi F., Giraudo G., Sena G., Castaldo P., Cardamone E., Portale G., Zuin M., Spolverato Y., Esposito M., Isernia R. M., Di Salvo M., Manunza R., Esposito G., Agus M., Asti E. L. G., Bernardi D. T., Tonucci T. P., Luppi D., Casadei M., Bonilauri S., Pezzolla A., Panebianco A., Laforgia R., De Luca M., Zese M., Parini D., Jovine E., De Sario G., Lombardi R., Aprea G., Palomba G., Capuano M., Argenio G., Orio G., Armellino M. F., Troian M., Guerra M., Nagliati C., Biloslavo A., Germani P., Aizza G., Monsellato I., Chahrour A. C., Anania G., Bombardini C., Bagolini F., Sganga G., Fransvea P., Bianchi V., Boati P., Ferrara F., Palmieri F., Cianci P., Gattulli D., Restini E., Cillara N., Cannavera A., Nita G. E., Sarnari J., Roscio F., Clerici F., Scandroglio I., Berti S., Cadeo A., Filippelli A., Conti L., Grassi C., Cattaneo G. M., Pighin M., Papis D., Gambino G., Bertino V., Schifano D., Prando D., Fogato L., Cavallo F., Ansaloni L., Picheo R., Pontarolo N., Depalma N., Spampinato M., D'Ugo S., Lepre L., Capponi M. G., Campa R. D., Sarro G., Dinuzzi V. P., Olmi S., Uccelli M., Ferrari D., Inama M., Moretto G., Fontana M., Favi F., Picariello E., Rampini A., Barberis A., Azzinnaro A., Oliva A., Totaro L., Benzoni I., Ranieri V., Capolupo G. T., Carannante F., Caricato M., Ronconi M., Casiraghi S., Casole G., Pantalone D., Alemanno G., Scheiterle M., Ceresoli M., Cereda M., Fumagalli C., Zanzi F., Bolzon S., Guerra E., Lecchi F., Cellerino P., Ardito A., Scaramuzzo R., Balla A., Lepiane P., Tartaglia N., Ambrosi A., Pavone G., Palini G. M., Veneroni S., Garulli G., Ricci C., Torre B., Russo I. S., Rottoli M., Tanzanu M., Belvedere A., Milone M., Manigrasso M., De Palma G. D., Piccoli M., Pattacini G. C., Magnone S., Bertoli P., Massucco P., Palisi M., Luzzi A. -P., Fleres F., Clarizia G., Spolini A., Kobe Y., Toma T., Shimamura F., Parker R., Ranketi S., Mitei M., Svagzdys S., Pauzas H., Zilinskas J., Poskus T., Kryzauskas M., Jakubauskas M., Zakaria A. D., Zakaria Z., Wong M. P. -K., Jusoh A. C., Zakaria M. N., Cruz D. R., Elizalde A. B. R., Reynaud A. B., Hernandez E. E. L., Monroy J. M. V. P., Hinojosa-Ugarte D., Quiodettis M., Du Bois M. E., Latorraca J., Major P., Pedziwiatr M., Pisarska-Adamczyk M., Waledziak M., Kwiatkowski A., Czyzykowski L., da Costa S. D., Pereira B., Ferreira A. R. O., Almeida F., Rocha R., Carneiro C., Perez D. P., Carvas J., Rocha C., Ferreira C., Marques R., Fernandes U., Leao P., Goulart A., Pereira R. G., Patrocinio S. D. D., de Mendonca N. G. G., Manso M. I. C., Morais H. M. C., Cardoso P. S., Calu V., Miron A., Toma E. A., Gachabayov M., Abdullaev A., Litvin A., Nechay T., Tyagunov A., Yuldashev A., Bradley A., Wilson M., Panyko A., Lateckova Z., Lacko V., Lesko D., Soltes M., Radonak J., Turrado-Rodriguez V., Termes-Serra R., Morales-Sevillano X., Lapolla P., Mingoli A., Brachini G., Degiuli M., Sofia S., Reddavid R., de Manzoni Garberini A., Buffone A., del Pozo E. P., Aparicio-Sanchez D., Dos Barbeito S., Estaire-Gomez M., Viton-Herrero R., de los Angeles Gil Olarte-Marquez M. a., Gil-Martinez J., Alconchel F., Nicolas-Lopez T., Rahy-Martin A. C., Pelloni M., Banolas-Suarez R., Mendoza-Moreno F., Nisa F. G. -M., Diez-Alonso M., Rodas M. E. V., Agundez M. C., Andres M. I. P., Moreira C. C. L., Perez A. L., Ponce I. A., Gonzalez-Castillo A. M., Membrilla-Fernandez E., Salvans S., Serradilla-Martin M., Pardo P. S., Rivera-Alonso D., Dziakova J., Huguet J. M., Valle N. P., Ruiz E. C., Valcarcel C. R., Moreno C. R., Salazar Y. T. M., Garcia J. J. R., Mico S. S., Lopez J. R., Farre S. P., Gomez M. S., Petit N. M., Titos-Garcia A., Aranda-Narvaez J. M., Romacho-Lopez L., Sanchez-Guillen L., Aranaz-Ostariz V., Bosch-Ramirez M., Martinez-Perez A., Martinez-Lopez E., Sebastian-Tomas J. C., Jimenez-Riera G., Jimenez-Vega J., Cuellar J. A. N., Campos-Serra A., Munoz-Campana A., Gracia-Roman R., Alegre J. M., Pinto F. L., O'Sullivan S. N., Antona F. B., Jimenez B. M., Lopez-Sanchez J., Carmona Z. G., Fernandez R. T., Sierra I. B., de Leon L. R. G., Moreno V. P., Iglesias E., Cumplido P. L., Bravo A. A., Simo I. R., Dominguez C. L., Caamano A. G., Lozano R. C., Martinez M. D., Torres A. N., de Quiros J. T. M. B., Pellino G., Cloquell M. M., Moller E. G., Jalal-Eldin S., Abdoun A. K., Hamid H. K. S., Lohsiriwat V., Mongkhonsupphawan A., Baraket O., Ayed K., Abbassi I., Ali A. B., Ammar H., Kchaou A., Tlili A., Zribi I., Colak E., Polat S., Koylu Z. A., Guner A., Usta M. A., Reis M. E., Mantoglu B., Gonullu E., Akin E., Altintoprak F., Bayhan Z., Firat N., Isik A., Memis U., Bayrak M., Altintas Y., Kara Y., Bozkurt M. A., Kocatas A., Das K., Seker A., Ozer N., Atici S. D., Tuncer K., Kaya T., Ozkan Z., Ilhan O., Agackiran I., Uzunoglu M. Y., Demirbas E., Altinel Y., Meric S., Hacim N. A., Uymaz D. S., Omarov N., Balik E., Tebala G. D., Khalil H., Rana M., Khan M., Florence C., Swaminathan C., Leo C. A., Liasis L., Watfah J., Trostchansky I., Delgado E., Pontillo M., Latifi R., Coimbra R., Edwards S., Lopez A., Velmahos G., Dorken A., Gebran A., Palmer A., Oury J., Bardes J. M., Seng S. S., Coffua L. S., Ratnasekera A., Egodage T., Echeverria-Rosario K., Armento I., Napolitano L. M., Sangji N. F., Hemmila M., Quick J. A., Austin T. R., Hyman T. S., Curtiss W., McClure A., Cairl N., Biffl W. L., Truong H. P., Schaffer K., Reames S., Banchini F., Capelli P., Coccolini F., Sartelli M., Bravi F., Vallicelli C., Agnoletti V., Baiocchi G. L., Catena F., Perrone, G, Giuffrida, M, Abu-Zidan, F, Kruger, V, Livrini, M, Petracca, G, Rossi, G, Tarasconi, A, Tian, B, Bonati, E, Mentz, R, Mazzini, F, Campana, J, Gasser, E, Kafka-Ritsch, R, Felsenreich, D, Dawoud, C, Riss, S, Gomes, C, Gomes, F, Gonzaga, R, Canton, C, Pereira, B, Fraga, G, Zem, L, Cordeiro-Fonseca, V, de Mesquita Tauil, R, Atanasov, B, Belev, N, Kovachev, N, Melendez, L, Dimova, A, Dimov, S, Zelic, Z, Augustin, G, Bogdanic, B, Moric, T, Chouillard, E, Bajul, M, De Simone, B, Panis, Y, Esposito, F, Notarnicola, M, Lauka, L, Fabbri, A, Hentati, H, Fnaiech, I, Aurelien, V, Bougard, M, Roulet, M, Demetrashvili, Z, Pipia, I, Merabishvili, G, Bouliaris, K, Koukoulis, G, Doudakmanis, C, Xenaki, S, Chrysos, E, Kokkinakis, S, Vassiliu, P, Michalopoulos, N, Margaris, I, Kechagias, A, Avgerinos, K, Katunin, J, Lostoridis, E, Nagorni, E, Pujante, A, Mulita, F, Maroulis, I, Vailas, M, Marinis, A, Siannis, I, Bourbouteli, E, Manatakis, D, Tasis, N, Acheimastos, V, Maria, S, Stylianos, K, Kuzeridis, H, Korkolis, D, Fradelos, E, Kavalieratos, G, Petropoulou, T, Polydorou, A, Papacostantinou, I, Triantafyllou, T, Kimpizi, D, Theodorou, D, Toutouzas, K, Chamzin, A, Frountzas, M, Schizas, D, Karavokyros, I, Syllaios, A, Charalabopoulos, A, Boura, M, Baili, E, Ioannidis, O, Loutzidou, L, Anestiadou, E, Tsouknidas, I, Petrakis, G, Polenta, E, Bains, L, Gupta, R, Singh, S, Khanduri, A, Bala, M, Kedar, A, Pisano, M, Podda, M, Pisanu, A, Martines, G, Trigiante, G, Lantone, G, Agrusa, A, Di Buono, G, Buscemi, S, Veroux, M, Gioco, R, Veroux, G, Oragano, L, Zonta, S, Lovisetto, F, Feo, C, Pesce, A, Fabbri, N, Marino, F, Perrone, F, Vincenti, L, Papagni, V, Picciariello, A, Rossi, S, Picardi, B, Del Monte, S, Visconti, D, Osella, G, Petruzzelli, L, Pignata, G, Andreuccetti, J, D'Alessio, R, Buonfantino, M, Guaitoli, E, Spinelli, S, Sampietro, G, Corbellini, C, Lorusso, L, Frontali, A, Pezzoli, I, Bonomi, A, Chierici, A, Cotsoglou, C, Manca, G, Delvecchio, A, Musa, N, Casati, M, Letizia, L, Abate, E, Ercolani, G, D'Acapito, F, Solaini, L, Guercioni, G, Cicconi, S, Sasia, D, Borghi, F, Giraudo, G, Sena, G, Castaldo, P, Cardamone, E, Portale, G, Zuin, M, Spolverato, Y, Esposito, M, Isernia, R, Di Salvo, M, Manunza, R, Esposito, G, Agus, M, Asti, E, Bernardi, D, Tonucci, T, Luppi, D, Casadei, M, Bonilauri, S, Pezzolla, A, Panebianco, A, Laforgia, R, De Luca, M, Zese, M, Parini, D, Jovine, E, De Sario, G, Lombardi, R, Aprea, G, Palomba, G, Capuano, M, Argenio, G, Orio, G, Armellino, M, Troian, M, Guerra, M, Nagliati, C, Biloslavo, A, Germani, P, Aizza, G, Monsellato, I, Chahrour, A, Anania, G, Bombardini, C, Bagolini, F, Sganga, G, Fransvea, P, Bianchi, V, Boati, P, Ferrara, F, Palmieri, F, Cianci, P, Gattulli, D, Restini, E, Cillara, N, Cannavera, A, Nita, G, Sarnari, J, Roscio, F, Clerici, F, Scandroglio, I, Berti, S, Cadeo, A, Filippelli, A, Conti, L, Grassi, C, Cattaneo, G, Pighin, M, Papis, D, Gambino, G, Bertino, V, Schifano, D, Prando, D, Fogato, 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Olarte-Marquez, M, Gil-Martinez, J, Alconchel, F, Nicolas-Lopez, T, Rahy-Martin, A, Pelloni, M, Banolas-Suarez, R, Mendoza-Moreno, F, Nisa, F, Diez-Alonso, M, Rodas, M, Agundez, M, Andres, M, Moreira, C, Perez, A, Ponce, I, Gonzalez-Castillo, A, Membrilla-Fernandez, E, Salvans, S, Serradilla-Martin, M, Pardo, P, Rivera-Alonso, D, Dziakova, J, Huguet, J, Valle, N, Ruiz, E, Valcarcel, C, Moreno, C, Salazar, Y, Garcia, J, Mico, S, Lopez, J, Farre, S, Gomez, M, Petit, N, Titos-Garcia, A, Aranda-Narvaez, J, Romacho-Lopez, L, Sanchez-Guillen, L, Aranaz-Ostariz, V, Bosch-Ramirez, M, Martinez-Perez, A, Martinez-Lopez, E, Sebastian-Tomas, J, Jimenez-Riera, G, Jimenez-Vega, J, Cuellar, J, Campos-Serra, A, Munoz-Campana, A, Gracia-Roman, R, Alegre, J, Pinto, F, O'Sullivan, S, Antona, F, Jimenez, B, Lopez-Sanchez, J, Carmona, Z, Fernandez, R, Sierra, I, de Leon, L, Moreno, V, Iglesias, E, Cumplido, P, Bravo, A, Simo, I, Dominguez, C, Caamano, A, Lozano, R, Martinez, M, Torres, A, de Quiros, J, Pellino, G, Cloquell, M, Moller, E, Jalal-Eldin, S, Abdoun, A, Hamid, H, Lohsiriwat, V, Mongkhonsupphawan, A, Baraket, O, Ayed, K, Abbassi, I, Ali, A, Ammar, H, Kchaou, A, Tlili, A, Zribi, I, Colak, E, Polat, S, Koylu, Z, Guner, A, Usta, M, Reis, M, Mantoglu, B, Gonullu, E, Akin, E, Altintoprak, F, Bayhan, Z, Firat, N, Isik, A, Memis, U, Bayrak, M, Altintas, Y, Kara, Y, Bozkurt, M, Kocatas, A, Das, K, Seker, A, Ozer, N, Atici, S, Tuncer, K, Kaya, T, Ozkan, Z, Ilhan, O, Agackiran, I, Uzunoglu, M, Demirbas, E, Altinel, Y, Meric, S, Hacim, N, Uymaz, D, Omarov, N, Balik, E, Tebala, G, Khalil, H, Rana, M, Khan, M, Florence, C, Swaminathan, C, Leo, C, Liasis, L, Watfah, J, Trostchansky, I, Delgado, E, Pontillo, M, Latifi, R, Coimbra, R, Edwards, S, Lopez, A, Velmahos, G, Dorken, A, Gebran, A, Palmer, A, Oury, J, Bardes, J, Seng, S, Coffua, L, Ratnasekera, A, Egodage, T, Echeverria-Rosario, K, Armento, I, Napolitano, L, Sangji, N, Hemmila, M, Quick, J, Austin, T, Hyman, T, Curtiss, W, Mcclure, A, Cairl, N, Biffl, W, Truong, H, Schaffer, K, Reames, S, Banchini, F, Capelli, P, Coccolini, F, Sartelli, M, Bravi, F, Vallicelli, C, Agnoletti, V, Baiocchi, G, Catena, F, Perrone G., Giuffrida M., Abu-Zidan F., Kruger V. F., Livrini M., Petracca G. L., Rossi G., Tarasconi A., Tian B. W. C. A., Bonati E., Mentz R., Mazzini F. N., Campana J. P., Gasser E., Kafka-Ritsch R., Felsenreich D. M., Dawoud C., Riss S., Gomes C. A., Gomes F. C., Gonzaga R. A. T., Canton C. A. B., Pereira B. M., Fraga G. P., Zem L. G., Cordeiro-Fonseca V., de Mesquita Tauil R., Atanasov B., Belev N., Kovachev N., Melendez L. J. J., Dimova A., Dimov S., Zelic Z., Augustin G., Bogdanic B., Moric T., Chouillard E., Bajul M., De Simone B., Panis Y., Esposito F., Notarnicola M., Lauka L., Fabbri A., Hentati H., Fnaiech I., Aurelien V., Bougard M., Roulet M., Demetrashvili Z., Pipia I., Merabishvili G., Bouliaris K., Koukoulis G., Doudakmanis C., Xenaki S., Chrysos E., Kokkinakis S., Vassiliu P., Michalopoulos N., Margaris I., Kechagias A., Avgerinos K., Katunin J., Lostoridis E., Nagorni E. -A., Pujante A., Mulita F., Maroulis I., Vailas M., Marinis A., Siannis I., Bourbouteli E., Manatakis D. K., Tasis N., Acheimastos V., Maria S., Stylianos K., Kuzeridis H., Korkolis D., Fradelos E., Kavalieratos G., Petropoulou T., Polydorou A., Papacostantinou I., Triantafyllou T., Kimpizi D., Theodorou D., Toutouzas K., Chamzin A., Frountzas M., Schizas D., Karavokyros I., Syllaios A., Charalabopoulos A., Boura M., Baili E., Ioannidis O., Loutzidou L., Anestiadou E., Tsouknidas I., Petrakis G., Polenta E., Bains L., Gupta R., Singh S. K., Khanduri A., Bala M., Kedar A., Pisano M., Podda M., Pisanu A., Martines G., Trigiante G., Lantone G., Agrusa A., Di Buono G., Buscemi S., Veroux M., Gioco R., Veroux G., Oragano L., Zonta S., Lovisetto F., Feo C. V., Pesce A., Fabbri N., Marino F., Perrone F., Vincenti L., Papagni V., Picciariello A., Rossi S., Picardi B., Del Monte S. R., Visconti D., Osella G., Petruzzelli L., Pignata G., Andreuccetti J., D'Alessio R., Buonfantino M., Guaitoli E., Spinelli S., Sampietro G. M., Corbellini C., Lorusso L., Frontali A., Pezzoli I., Bonomi A., Chierici A., Cotsoglou C., Manca G., Delvecchio A., Musa N., Casati M., Letizia L., Abate E., Ercolani G., D'Acapito F., Solaini L., Guercioni G., Cicconi S., Sasia D., Borghi F., Giraudo G., Sena G., Castaldo P., Cardamone E., Portale G., Zuin M., Spolverato Y., Esposito M., Isernia R. M., Di Salvo M., Manunza R., Esposito G., Agus M., Asti E. L. G., Bernardi D. T., Tonucci T. P., Luppi D., Casadei M., Bonilauri S., Pezzolla A., Panebianco A., Laforgia R., De Luca M., Zese M., Parini D., Jovine E., De Sario G., Lombardi R., Aprea G., Palomba G., Capuano M., Argenio G., Orio G., Armellino M. F., Troian M., Guerra M., Nagliati C., Biloslavo A., Germani P., Aizza G., Monsellato I., Chahrour A. C., Anania G., Bombardini C., Bagolini F., Sganga G., Fransvea P., Bianchi V., Boati P., Ferrara F., Palmieri F., Cianci P., Gattulli D., Restini E., Cillara N., Cannavera A., Nita G. E., Sarnari J., Roscio F., Clerici F., Scandroglio I., Berti S., Cadeo A., Filippelli A., Conti L., Grassi C., Cattaneo G. M., Pighin M., Papis D., Gambino G., Bertino V., Schifano D., Prando D., Fogato L., Cavallo F., Ansaloni L., Picheo R., Pontarolo N., Depalma N., Spampinato M., D'Ugo S., Lepre L., Capponi M. G., Campa R. D., Sarro G., Dinuzzi V. P., Olmi S., Uccelli M., Ferrari D., Inama M., Moretto G., Fontana M., Favi F., Picariello E., Rampini A., Barberis A., Azzinnaro A., Oliva A., Totaro L., Benzoni I., Ranieri V., Capolupo G. T., Carannante F., Caricato M., Ronconi M., Casiraghi S., Casole G., Pantalone D., Alemanno G., Scheiterle M., Ceresoli M., Cereda M., Fumagalli C., Zanzi F., Bolzon S., Guerra E., Lecchi F., Cellerino P., Ardito A., Scaramuzzo R., Balla A., Lepiane P., Tartaglia N., Ambrosi A., Pavone G., Palini G. M., Veneroni S., Garulli G., Ricci C., Torre B., Russo I. S., Rottoli M., Tanzanu M., Belvedere A., Milone M., Manigrasso M., De Palma G. D., Piccoli M., Pattacini G. C., Magnone S., Bertoli P., Massucco P., Palisi M., Luzzi A. -P., Fleres F., Clarizia G., Spolini A., Kobe Y., Toma T., Shimamura F., Parker R., Ranketi S., Mitei M., Svagzdys S., Pauzas H., Zilinskas J., Poskus T., Kryzauskas M., Jakubauskas M., Zakaria A. D., Zakaria Z., Wong M. P. -K., Jusoh A. C., Zakaria M. N., Cruz D. R., Elizalde A. B. R., Reynaud A. B., Hernandez E. E. L., Monroy J. M. V. P., Hinojosa-Ugarte D., Quiodettis M., Du Bois M. E., Latorraca J., Major P., Pedziwiatr M., Pisarska-Adamczyk M., Waledziak M., Kwiatkowski A., Czyzykowski L., da Costa S. D., Pereira B., Ferreira A. R. O., Almeida F., Rocha R., Carneiro C., Perez D. P., Carvas J., Rocha C., Ferreira C., Marques R., Fernandes U., Leao P., Goulart A., Pereira R. G., Patrocinio S. D. D., de Mendonca N. G. G., Manso M. I. C., Morais H. M. C., Cardoso P. S., Calu V., Miron A., Toma E. A., Gachabayov M., Abdullaev A., Litvin A., Nechay T., Tyagunov A., Yuldashev A., Bradley A., Wilson M., Panyko A., Lateckova Z., Lacko V., Lesko D., Soltes M., Radonak J., Turrado-Rodriguez V., Termes-Serra R., Morales-Sevillano X., Lapolla P., Mingoli A., Brachini G., Degiuli M., Sofia S., Reddavid R., de Manzoni Garberini A., Buffone A., del Pozo E. P., Aparicio-Sanchez D., Dos Barbeito S., Estaire-Gomez M., Viton-Herrero R., de los Angeles Gil Olarte-Marquez M. a., Gil-Martinez J., Alconchel F., Nicolas-Lopez T., Rahy-Martin A. C., Pelloni M., Banolas-Suarez R., Mendoza-Moreno F., Nisa F. G. -M., Diez-Alonso M., Rodas M. E. V., Agundez M. C., Andres M. I. P., Moreira C. C. L., Perez A. L., Ponce I. A., Gonzalez-Castillo A. M., Membrilla-Fernandez E., Salvans S., Serradilla-Martin M., Pardo P. S., Rivera-Alonso D., Dziakova J., Huguet J. M., Valle N. P., Ruiz E. C., Valcarcel C. R., Moreno C. R., Salazar Y. T. M., Garcia J. J. R., Mico S. S., Lopez J. R., Farre S. P., Gomez M. S., Petit N. M., Titos-Garcia A., Aranda-Narvaez J. M., Romacho-Lopez L., Sanchez-Guillen L., Aranaz-Ostariz V., Bosch-Ramirez M., Martinez-Perez A., Martinez-Lopez E., Sebastian-Tomas J. C., Jimenez-Riera G., Jimenez-Vega J., Cuellar J. A. N., Campos-Serra A., Munoz-Campana A., Gracia-Roman R., Alegre J. M., Pinto F. L., O'Sullivan S. N., Antona F. B., Jimenez B. M., Lopez-Sanchez J., Carmona Z. G., Fernandez R. T., Sierra I. B., de Leon L. R. G., Moreno V. P., Iglesias E., Cumplido P. L., Bravo A. A., Simo I. R., Dominguez C. L., Caamano A. G., Lozano R. C., Martinez M. D., Torres A. N., de Quiros J. T. M. B., Pellino G., Cloquell M. M., Moller E. G., Jalal-Eldin S., Abdoun A. K., Hamid H. K. S., Lohsiriwat V., Mongkhonsupphawan A., Baraket O., Ayed K., Abbassi I., Ali A. B., Ammar H., Kchaou A., Tlili A., Zribi I., Colak E., Polat S., Koylu Z. A., Guner A., Usta M. A., Reis M. E., Mantoglu B., Gonullu E., Akin E., Altintoprak F., Bayhan Z., Firat N., Isik A., Memis U., Bayrak M., Altintas Y., Kara Y., Bozkurt M. A., Kocatas A., Das K., Seker A., Ozer N., Atici S. D., Tuncer K., Kaya T., Ozkan Z., Ilhan O., Agackiran I., Uzunoglu M. Y., Demirbas E., Altinel Y., Meric S., Hacim N. A., Uymaz D. S., Omarov N., Balik E., Tebala G. D., Khalil H., Rana M., Khan M., Florence C., Swaminathan C., Leo C. A., Liasis L., Watfah J., Trostchansky I., Delgado E., Pontillo M., Latifi R., Coimbra R., Edwards S., Lopez A., Velmahos G., Dorken A., Gebran A., Palmer A., Oury J., Bardes J. M., Seng S. S., Coffua L. S., Ratnasekera A., Egodage T., Echeverria-Rosario K., Armento I., Napolitano L. M., Sangji N. F., Hemmila M., Quick J. A., Austin T. R., Hyman T. S., Curtiss W., McClure A., Cairl N., Biffl W. L., Truong H. P., Schaffer K., Reames S., Banchini F., Capelli P., Coccolini F., Sartelli M., Bravi F., Vallicelli C., Agnoletti V., Baiocchi G. L., and Catena F.
Abstract: Background: Literature suggests colonic resection and primary anastomosis (RPA) instead of Hartmann’s procedure (HP) for the treatment of left-sided colonic emergencies. We aim to evaluate the surgical options globally used to treat patients with acute left-sided colonic emergencies and the factors that leading to the choice of treatment, comparing HP and RPA. Methods: This is a prospective, international, multicenter, observational study registered on ClinicalTrials.gov. A total 1215 patients with left-sided colonic emergencies who required surgery were included from 204 centers during the period of March 1, 2020, to May 31, 2020. with a 1-year follow-up. Results: 564 patients (43.1%) were females. The mean age was 65.9 ± 15.6 years. HP was performed in 697 (57.3%) patients and RPA in 384 (31.6%) cases. Complicated acute diverticulitis was the most common cause of left-sided colonic emergencies (40.2%), followed by colorectal malignancy (36.6%). Severe complications (Clavien-Dindo ≥ 3b) were higher in the HP group (P < 0.001). 30-day mortality was higher in HP patients (13.7%), especially in case of bowel perforation and diffused peritonitis. 1-year follow-up showed no differences on ostomy reversal rate between HP and RPA. (P = 0.127). A backward likelihood logistic regression model showed that RPA was preferred in younger patients, having low ASA score (≤ 3), in case of large bowel obstruction, absence of colonic ischemia, longer time from admission to surgery, operating early at the day working hours, by a surgeon who performed more than 50 colorectal resections. Conclusions: After 100 years since the first Hartmann’s procedure, HP remains the most common treatment for left-sided colorectal emergencies. Treatment’s choice depends on patient characteristics, the time of surgery and the experience of the surgeon. RPA should be considered as the gold standard for surgery, with HP being an exception.
Published: 2024

2. Detection and Management of Amyloid-Related Imaging Abnormalities in Patients with Alzheimer’s Disease Treated with Anti-Amyloid Beta Therapy

Author: Barakos, Jerome, Purcell, D., Suhy, J., Chalkias, S., Burkett, P., Grassi, C. Marsica, Castrillo-Viguera, C., Rubino, I., and Vijverberg, E.
Published: 2022
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3. Combined molecular and mathematical analysis of long noncoding RNAs expression in fine needle aspiration biopsies as novel tool for early diagnosis of thyroid cancer

Author: Possieri, C., Locantore, P., Salis, C., Bacci, L., Aiello, A., Fadda, G., De Crea, C., Raffaelli, M., Bellantone, R., Grassi, C., Strigari, L., Farsetti, A., Pontecorvi, A., and Nanni, S.
Published: 2021
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4. Rotational Damping and Compound Formation in Warm Rotating Nuclei

Author: Matsuo, M., Leoni, S., Grassi, C., Vigezzi, E., Bracco, A., Dossing, T., and Herskind, B.
Subjects: Nuclear Theory
Abstract: The rotational damping width \Gamma_{rot} and the compound damping width \Gamma_{comp} are two fundamental quantities that characterize rapidly rotating compound nuclei having finite thermal excitation energy. A two-component structure in the strength function of consecutive E2 transitions reflects the two widths, and it causes characteristic features in the double and triple gamma-ray spectra. We discuss a new method to extract experimentally values of \Gamma_{rot} and \Gamma_{comp}. The first preliminary result of this method is presented., Comment: PDF, 8 pages, invited talk at the Conference on Frontiers of Nuclear Structure (FNS2002), August 2002, Berkeley
Published: 2002
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5. Opuntia ficus-indica (L.) Mill. growing in soil and containers for urban agriculture in developing areas

Author: Pardini, A., Massolino, F., and Grassi, C.
Published: 2017

6. Interannual Variation in Diet, Dietary Diversity, and Dietary Overlap in Three Sympatric Strepsirrhine Species in Southeastern Madagascar

Author: Erhart, E. M., Tecot, S. R., and Grassi, C.
Published: 2018
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7. Near-infrared controlled release of mesenchymal stem cells secretome from bioprinted graphenebased microbeads for nerve regeneration

Author: Perini, Giordano, Palmieri, V., D'Ascenzo, Marcello, Colussi, Claudia, Grassi, Claudio, Friggeri, G., Augello, A., Cui, Lishan, Papi, Massimiliano, De Spirito, Marco, Perini G. (ORCID:0000-0001-9452-8479), D'Ascenzo M. (ORCID:0000-0003-0073-412X), Colussi C., Grassi C. (ORCID:0000-0001-7253-1685), Cui L., Papi M. (ORCID:0000-0002-0029-1309), de Spirito M. (ORCID:0000-0003-4260-5107), Perini, Giordano, Palmieri, V., D'Ascenzo, Marcello, Colussi, Claudia, Grassi, Claudio, Friggeri, G., Augello, A., Cui, Lishan, Papi, Massimiliano, De Spirito, Marco, Perini G. (ORCID:0000-0001-9452-8479), D'Ascenzo M. (ORCID:0000-0003-0073-412X), Colussi C., Grassi C. (ORCID:0000-0001-7253-1685), Cui L., Papi M. (ORCID:0000-0002-0029-1309), and de Spirito M. (ORCID:0000-0003-4260-5107)
Abstract: Nerve damage is a prevalent and debilitating condition with limited treatment options. Recent years have seen an increased incidence of neural damage due to factors such as aging populations and traumatic brain injuries. Addressing the urgent need for effective therapies, this study explores the controlled delivery of mesenchymal stem cells (MSCs) secretome, a complex mixture of bioactive factors, which is currently being investigated for its potential in nerve regeneration. The secretome offers significant advantages over stem cells themselves, as it can be more easily characterized and controlled, enabling precise regulation of therapeutic interventions. However, the challenge lies in delivering the secretome specifically to the target anatomical region. To overcome this limitation, we propose a novel approach utilizing near-infrared (NIR) radiation-responsive bioprinted alginate-graphene oxide (AGO) microbeads. Graphene oxide (GO) is a highly biocompatible material with unique properties, including NIR responsiveness, enabling controlled release of therapeutic agents upon NIR exposure. We hypothesized that AGO microbeads could encapsulate MSCs secretome and release it in a controlled manner using NIR radiation. To investigate our hypothesis, controlled damage was induced to hippocampal neurons, and MSCs secretome was encapsulated within AGO microbeads. Subsequently, NIR radiation was applied to trigger the release of the secretome. We compared the efficacy of MSCs secretome with that of astrocytes, which also possess nerve growth and proliferation-promoting capabilities. Our findings demonstrated that the controlled release of MSCs secretome from AGO microbeads through non-invasive NIR radiation significantly promoted the proliferation and regeneration of neurons following nerve injury. AGO microbeads offer multiple advantages over conventional delivery methods, including precise control over the timing, location, and dosage of therapeutic agents. Furthermore, th
Published: 2023

8. The cross-rode between oxidative stress and inflammation in the auditory system damage: role of via glial cell and macrophages activation in ototoxicity.

Author: Paciello, F., Pisani, A., Rolesi, R., Ripoli, C., Grassi, C., and Fetoni, A. R.
Subjects: AUDITORY cortex, MACROPHAGES, NEUROGLIA, OXIDATIVE stress, CONFERENCES & conventions, INFLAMMATION, OTOTOXICITY
Abstract: Redox imbalance and inflammation have been proposed as the principal mechanisms of damage in the auditory system, resulting in functional alterations and hearing loss. Microglia and astrocytes play a crucial role in mediating oxidative/inflammatory injury in the central nervous system; however, the role of glial cells in the auditory damage is still elusive. In this study, we investigated glial-mediated responses to toxic injury in peripheral and central structures of the auditory pathway, i.e., the cochlea and the auditory cortex (ACx), in rats exposed to styrene, a volatile compound with well-known oto/neurotoxic properties. To this aim, male adult Wistar rats were treated with styrene (400 mg/kg daily for 3 weeks, 5/ days a week). At the end of treatment (day 21) electrophysiological, morphological, immunofluorescence and molecular analyses were performed in both the cochlea and in ACx samples to evaluate the mechanisms underlying styrene-in-duced oto/neurotoxicity in the auditory system. We showed that the oto/neurotoxic insult induced by styrene increases oxidative stress in both cochlea and ACx. This was associated with macrophages and glial cell activation, increased expression of inflammatory markers (i.e., pro-inflammatory cytokines and chemokine receptors) and alterations in con-nexin (Cxs) and pannexin (Panx) expression, likely responsible for dysregulation of the microglia/astrocyte network. Specifically, we found downregulation of Cx26 and Cx30 in the cochlea, and high level of Cx43 and Panx1 in the ACx. Collectively, our results provide novel evidence on the role of immune and glial cell activation in the oxidative/inflammatory damage induced by styrene in the auditory system at both peripheral and central levels, also involving alterations of gap junction networks. Our data suggest that targeting glial cells and connexin/pannexin expression might be useful to attenuate oxidative/inflammatory damage in the auditory system. [ABSTRACT FROM AUTHOR]
Published: 2024

9. Post-COVID condition in adults and children living in the same household in Italy: a prospective cohort study using the ISARIC global follow-up protocol

Author: Buonsenso, D, Munblit, D, Pazukhina, E, Ricchiuto, A, Sinatti, D, Zona, M, De Matteis, A, D’Ilario, F, Gentili, C, Lanni, R, Rongai, T, del Balzo, P, Fonte, MT, Valente, M, Zampino, G, De Rose, C, Sigfrid, L, Valentini, P, Sani, I, La Cava, G, Castronuovo, S, Capodici, I, Zirletta, E, Costabile, L, Martino, D, Arnaboldi, L, Carbone, MC, Sebastianelli, R, Ciuffo, C, Marano, D, Grassi, C, La Bella, I, Sozio, L, and FIMP-Roma
Subjects: children, Settore MED/38 - PEDIATRIA GENERALE E SPECIALISTICA, post-COVID “long-haulers”, Pediatrics, Perinatology and Child Health, adults, COVID-19, long COVID
Abstract: BackgroundEmerging evidence shows that both adults and children may develop post-acute sequelae of SARS-CoV-2 infection (PASC). The aim of this study is to characterise and compare long-term post-SARS-CoV-2 infection outcomes in adults and children in a defined region in Italy.MethodsA prospective cohort study including children (≤18 years old) with PCR-confirmed SARS-CoV-2 infection and their household members. Participants were assessed via telephone and face-to-face visits up to 12 months post-SARS-CoV-2 diagnosis of household index case, using the ISARIC COVID-19 follow-up survey.ResultsOf 507 participants from 201 households, 56.4% (286/507) were children, 43.6% (221/507) adults. SARS-CoV-2 positivity was 87% (249/286) in children, and 78% (172/221) in adults. The mean age of PCR positive children was 10.4 (SD = 4.5) and of PCR positive adults was 44.5 years (SD = 9.5), similar to the PCR negative control groups [children 10.5 years (SD = 3.24), adults 42.3 years (SD = 9.06)]. Median follow-up post-SARS-CoV-2 diagnosis was 77 days (IQR 47–169). A significantly higher proportion of adults compared to children reported at least one persistent symptom (67%, 68/101 vs. 32%, 57/179, p < 0.001) at the first follow up. Adults had more frequently coexistence of several symptom categories at both follow-up time-points. Female gender was identified as a risk factor for PASC in adults (p 0.02 at 1–3 months and p 0.01 at 6–9 months follow up), but not in children. We found no significant correlation between adults and children symptoms. In the paediatric group, there was a significant difference in persisting symptoms between those with confirmed SARS-CoV-2 infection compared to controls at 1–3 months follow up, but not at 6–9 months. Conversely, positive adults had a higher frequency of persisting symptoms at both follow-up assessments.ConclusionOur data highlights that children can experience persistent multisystemic symptoms months after diagnosis of mild acute SARS-CoV-2 infection, although less frequently and less severely than co-habitant adults. There was no correlation between symptoms experienced by adults and children living in the same household. Our data highlights an urgent need for studies to characterise PASC in whole populations and the wider impact on families.
Published: 2022

10. OC-006 LAPAROSCOPIC TOTALLY EXTRA-PERITONEAL REPAIR OF BILATERAL INGUINAL HERNIAS WITH A LARGE SINGLE MESH: A SINGLE CENTER STUDY WITH MINIMUM 5 YEARS FOLLOW UP

Author: Shaltiel, T, primary, Grassi, C, additional, Esepkina, O, additional, and Issa, N, additional
Published: 2022
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11. Critical Role of d -Serine Signaling in Synaptic Plasticity Relevant to Cocaine Addiction

Author: D’Ascenzo, M., primary, Mainardi, M., additional, and Grassi, C., additional
Published: 2017
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12. List of Contributors

Author: Alves, C.J., primary, Anglard, P., additional, Anier, K., additional, Armstrong, R.A., additional, Bachtell, R.K., additional, Bakshi, K., additional, Barbanti, P., additional, Barrio, P., additional, Batalla, A., additional, Becker, J.B., additional, Bekker, A., additional, Benkelfat, C., additional, Bergman, J., additional, Bhattacharya, P., additional, Bisagno, Veronica, additional, Brimijoin, S., additional, Brown, Z.J., additional, Buffalari, D., additional, Bühler, K.-M., additional, Caffino, L., additional, Cafforio, G., additional, Camarini, R., additional, Carvalho, V.M., additional, Cepko, L.C.S., additional, Chen, C.-C., additional, Chu, X.-P., additional, Corbit, L.H., additional, Crofton, E.J., additional, Crunelle, C.L., additional, Cunha, P.J., additional, Cunha-Oliveira, T., additional, Currie, P.J., additional, D’Ascenzo, M., additional, Dieckmann, L.H.J., additional, von Diemen, L., additional, Dumont, E.C., additional, Eagle, A.L., additional, Eipper, B.A., additional, Eipper-Mains, J.E., additional, Engeln, M., additional, Erb, S., additional, Farré, A., additional, Farré, M., additional, Felts, A.S., additional, Fofi, L., additional, Foster, J.D., additional, Fox, H.C., additional, Frankfurt, M., additional, Freissmuth, M., additional, Fuchs, R.A., additional, Fumagalli, F., additional, Gajewski, P.A., additional, Galaj, E., additional, Galduróz, J.C.F., additional, Garling, E.E., additional, Gentile, T.A., additional, Giannotti, G., additional, Girault, J.-A., additional, Glass, J.D., additional, Goncalves, P.D., additional, González-Duarte, A., additional, Gonzalez-Nunez, V., additional, Gould, R.W., additional, Grassi, C., additional, Green, T.A., additional, Green-Sadan, T., additional, Gu, H.H., additional, Guan, Xiaowei, additional, Halbout, B., additional, Han, D.D., additional, Henry, L.K., additional, Pérez de Heredia, J.L., additional, Higginbotham, J.A., additional, Hofmaier, T., additional, Holy, M., additional, Hsu, K.-S., additional, Huang, C.-C., additional, James, J., additional, Jones, A.W., additional, Jones, C.K., additional, Kalda, A., additional, Kearns, D.N., additional, Kerver, H.N., additional, Kessler, F., additional, Kohut, S.J., additional, Krnjević, K., additional, Kucab, P., additional, Kudlacek, O., additional, Kupferschmidt, D.A., additional, Kuzhikandathil, E.V., additional, Lee, M.R., additional, Leggio, L., additional, Lever, J.R., additional, Lever, S.Z., additional, Leyton, M., additional, Li, J.-X., additional, Lima, D.R., additional, Lobo, M.K., additional, López-Moreno, J.A., additional, López-Pelayo, H., additional, Lovejoy, D.A., additional, Luf, A., additional, Lugon, M.D.M.V., additional, Lyons, C.E., additional, Magalhães, A., additional, Magalhães, P.V.S., additional, Mainardi, M., additional, Mains, R.E., additional, Mantsch, J.R., additional, Marcourakis, T., additional, Marhe, R., additional, Matthys, F., additional, El Mestikawy, S., additional, Milivojevic, V., additional, Miller, D.K., additional, Min, M.O., additional, Minnes, S., additional, Mitchell, M.R., additional, Monteiro, P.R., additional, Murthy, V., additional, Muschamp, J.W., additional, Nagy, C., additional, Nakamura-Palacios, E.M., additional, Narvaez, J.C.M., additional, Normandeau, C.P., additional, Ornell, F., additional, Orsini, C.A., additional, Ostlund, S.B., additional, Otkins, J., additional, Patel, V.B., additional, Pelição, F.S., additional, Pereira, S.P., additional, Peres, M.D., additional, Potenza, M.N., additional, Preedy, V.R., additional, Prosser, R.A., additional, Quednow, B.B., additional, Rajendram, R., additional, Ramos, A.C., additional, Ranaldi, R., additional, Rangel-Barajas, C., additional, Rebec, G.V., additional, Robison, A.J., additional, Rodríguez, R.E., additional, Rohn, M.C.H., additional, Roth-Deri, I., additional, Scala, S.G., additional, Scavone, C., additional, Schellekens, A., additional, Scherer, J., additional, Schmid, R., additional, Scurlock, R.D., additional, Setlow, B., additional, Simmons, S.J., additional, Singer, L.T., additional, Sinha, R., additional, Sitte, H.H., additional, Smart, K., additional, Stockner, T., additional, Summavielle, T., additional, Szumlinski, K.K., additional, Tanda, G., additional, Torrens, M., additional, Tunstall, B.J., additional, Urbano, F.J., additional, Vaughan, R.A., additional, Verhaeghe, M., additional, Vonmoos, M., additional, Wagner, J.J., additional, Wang, Y., additional, Xi, Z.-X., additional, Xu, Y., additional, Yadid, G., additional, Ye, J.-H., additional, Yoon, S., additional, Zallar, L.J., additional, Zhan, Chang-Guo, additional, Zhang, H.-Y., additional, Zhang, Y., additional, Zheng, Fang, additional, Zuo, W., additional, and Zwiller, J., additional
Published: 2017
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13. Signaling through estrogen receptors modulates long non-coding RNAs in prostate cancer

Author: Nanni, S, Bacci, L, Aiello, A, Re, A, Salis, C, Grassi, C, Pontecorvi, A, Gaetano, C, Farsetti, A, Nanni S (ORCID:0000-0002-3320-1584), Bacci L, Re A, Salis C, Grassi C (ORCID:0000-0001-7253-1685), Pontecorvi A (ORCID:0000-0003-0570-6865), Nanni, S, Bacci, L, Aiello, A, Re, A, Salis, C, Grassi, C, Pontecorvi, A, Gaetano, C, Farsetti, A, Nanni S (ORCID:0000-0002-3320-1584), Bacci L, Re A, Salis C, Grassi C (ORCID:0000-0001-7253-1685), and Pontecorvi A (ORCID:0000-0003-0570-6865)
Abstract: Prostate cancer (PCa) is a sex-steroid hormone-dependent cancer in which estrogens play a critical role in both initiation and progression. Recently, several long non-coding RNAs (lncRNAs) have been associated with PCa and are supposedly playing a pivotal role in the biology and progression of this type of cancer. In this review, we focused on some lncRNAs that are known for their androgen and estrogen transcriptional responsiveness in PCa. Specifically, we summarized recent pieces of evidence about lncRNAs NEAT1, H19, MALAT1, and HOTAIR, in estrogen signaling, emphasizing their role in PCa progression and the acquisition of a castration-resistant phenotype. Here, the reader will find information about lncRNAs present in estrogen-dependent transcriptional complexes. The potential role of lncRNA/estrogen signaling as a novel pathway for PCa treatment will be discussed
Published: 2020

14. Pulmonary Emphysema: What’s Going On

Author: Grassi, C., Luisetti, M., Corsico, R., editor, Grassi, C., editor, Travis, J., editor, Casali, L., editor, and Luisetti, M., editor
Published: 1992
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15. Manejo nutricional de ascitis quilosa: Serie de casos y revisión de la literatura

Author: Bruno Grassi C, Camila Reimer C, Paulina Jofré M, María José Valenzuela P, and Silvana Valdés B
Subjects: Soporte nutricional, Nutrition and Dietetics, Ascitis quilosa, Terapia nutricional, Food Science
Abstract: RESUMEN Las ascitis quilosa (AQ) es una entidad poco común producida por el acúmulo de linfa en la cavidad peritoneal. Su incidencia se describe en aumento progresivo, asociándose a una mortalidad de 40-70%. Se incluyeron 3 pacientes con diagnóstico de AQ evaluados en la visita de asistencia nutricional del Hospital Clínico de la Universidad Católica (UC) durante el año 2019. Caso 1: Paciente mujer de 47 años, consulta por dolor abdominal agudo realizándose apendicectomía. Estudio de líquido peritoneal con triglicéridos (TG) de 1.362 mg/dL. Inicia Nutrición Parenteral Total (NPTC) progresando luego a régimen oral. Estudio no revela lesiones de vasos linfáticos ni otras causas. Caso 2: Paciente varón de 68 años con cirrosis por alcohol, Child Pugh B. Ingresa por disnea y ascitis refractaria. Estudio de líquido ascítico y pleural, con TG de 439 mg/dL y 592 mg/dL respectivamente. Se manejó con toracocentesis y paracentesis evacuadoras, tratamiento con régimen hipograso y aporte de triglicéridos de cadena media (MCT) vía oral. Evolución tórpida requiriendo apoyo con NPTC, realizándose drenajes sucesivos, por lo que se instala TIPS. Caso 3: Paciente mujer de 63 años consulta por dolor hipogástrico con masa palpable subcostal derecha. Estudio confirma masa pancreática por lo que se realiza Whipple. Reingresa por náuseas y vómitos profusos, evidenciándose líquido ascítico con TG de 251 mg/dl. Se inicia NPTC, escasos débitos del drenaje iniciándose realimentación progresiva por vía oral. El análisis del líquido tras la paracentesis establece el diagnóstico de AQ pues la clínica es inespecífica. Las principales complicaciones están dadas por la pérdida de quilo: desnutrición, infecciones y sepsis. Las opciones de tratamiento incluyen: medidas dietéticas, fármacos e intervenciones percutáneas o quirúrgicas; siempre orientadas al alivio sintomático, con foco en tratar la causa. Si la tolerancia oral es óptima la primera medida es la supresión de la grasa y la suplementación con MCT para evitar déficit energético. Con el empleo de estas medidas se ha reportado el cierre espontáneo de fístulas y/o defectos de vasos linfáticos en un 75%-80%. Se concluye que no hay guías de recomendación y los estudios se basan en series de pocos casos clínicos. La ascitis quilosa es una entidad patológica rara, que representa una situación clínica crítica con consecuencias inmunológicas y nutricionales; y el tratamiento debe ser etiológico y el paso clave inicial es optimizar el estado nutricional del paciente.
Published: 2020

16. Extracellular tau oligomers affect extracellular glutamate handling by astrocytes through downregulation of GLT-1 expression and impairment of NKA1A2 function

Author: Li Puma, Domenica Donatella, Ripoli, Cristian, Puliatti, Giulia, Pastore, Francesco, Lazzarino, Giacomo, Tavazzi, Barbara, Arancio, O., Piacentini, Roberto, Grassi, Claudio, Li Puma D. D. (ORCID:0000-0001-6729-6967), Ripoli C. (ORCID:0000-0002-5315-0163), Puliatti G., Pastore F., Lazzarino G., Tavazzi B. (ORCID:0000-0001-8743-0895), Piacentini R. (ORCID:0000-0003-4215-1643), Grassi C. (ORCID:0000-0001-7253-1685), Li Puma, Domenica Donatella, Ripoli, Cristian, Puliatti, Giulia, Pastore, Francesco, Lazzarino, Giacomo, Tavazzi, Barbara, Arancio, O., Piacentini, Roberto, Grassi, Claudio, Li Puma D. D. (ORCID:0000-0001-6729-6967), Ripoli C. (ORCID:0000-0002-5315-0163), Puliatti G., Pastore F., Lazzarino G., Tavazzi B. (ORCID:0000-0001-8743-0895), Piacentini R. (ORCID:0000-0003-4215-1643), and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: Aims: Several studies reported that astrocytes support neuronal communication by the release of gliotransmitters, including ATP and glutamate. Astrocytes also play a fundamental role in buffering extracellular glutamate in the synaptic cleft, thus limiting the risk of excitotoxicity in neurons. We previously demonstrated that extracellular tau oligomers (ex-oTau), by specifically targeting astrocytes, affect glutamate-dependent synaptic transmission via a reduction in gliotransmitter release. The aim of this work was to determine if ex-oTau also impair the ability of astrocytes to uptake extracellular glutamate, thus further contributing to ex-oTau-dependent neuronal dysfunction. Methods: Primary cultures of astrocytes and organotypic brain slices were exposed to ex-oTau (200 nM) for 1 h. Extracellular glutamate buffering by astrocytes was studied by: Na+ imaging; electrophysiological recordings; high-performance liquid chromatography; Western blot and immunofluorescence. Experimental paradigms avoiding ex-oTau internalisation (i.e. heparin pre-treatment and amyloid precursor protein knockout astrocytes) were used to dissect intracellular vs extracellular effects of oTau. Results: Ex-oTau uploading in astrocytes significantly affected glutamate-transporter-1 expression and function, thus impinging on glutamate buffering activity. Ex-oTau also reduced Na-K-ATPase activity because of pump mislocalisation on the plasma membrane, with no significant changes in expression. This effect was independent of oTau internalisation and it caused Na+ overload and membrane depolarisation in ex-oTau-targeted astrocytes. Conclusions: Ex-oTau exerted a complex action on astrocytes, at both intracellular and extracellular levels. The net effect was dysregulated glutamate signalling in terms of both release and uptake that relied on reduced expression of glutamate-transporter-1, altered function and localisation of NKA1A1, and NKA1A2. Consequently, Na+ gradients and all Na+-depend
Published: 2022

17. Engineering a switchable single-chain TEV protease to control protein maturation in living neurons

Author: Renna, Pietro, Ripoli, Cristian, Dagliyan, O., Pastore, Francesco, Rinaudo, Marco, Re, Agnese, Paciello, Fabiola, Grassi, Claudio, Renna P., Ripoli C. (ORCID:0000-0002-5315-0163), Pastore F., Rinaudo M. (ORCID:0000-0002-6130-7335), Re A., Paciello F. (ORCID:0000-0002-8473-8074), Grassi C. (ORCID:0000-0001-7253-1685), Renna, Pietro, Ripoli, Cristian, Dagliyan, O., Pastore, Francesco, Rinaudo, Marco, Re, Agnese, Paciello, Fabiola, Grassi, Claudio, Renna P., Ripoli C. (ORCID:0000-0002-5315-0163), Pastore F., Rinaudo M. (ORCID:0000-0002-6130-7335), Re A., Paciello F. (ORCID:0000-0002-8473-8074), and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: Engineered proteases are promising tools to address physiological and pathophysiological questions as well as to develop new therapeutic approaches. Here we introduce a new genetically encoded engineered single-chain tobacco etch virus protease, allowing to control proprotein cleavage in different compartments of living mammalian cells. We demonstrated a set of controllable proteolytic effects, including cytosolic protein cleavage, inducible gene expression, and maturation of brain-derived neurotrophic factor (BDNF) in the secretory pathway thus showing the versatility of this technique. Of note, the secretory pathway exhibits different characteristics from the cytosol and it is difficult to target because inaccessible to some small molecules. We were able to induce ligand-mediated BDNF maturation and monitor its effects on dendritic spines in hippocampal pyramidal cells and in the mouse brain. This strategy paves the way to dissect proteolytic cleavage product signaling in various processes as well as for future therapeutic applications.
Published: 2022

18. Role of HSV-1 in Alzheimer's disease pathogenesis: A challenge for novel preventive/therapeutic strategies

Author: Protto, V., Marcocci, M. E., Miteva, M. T., Piacentini, Roberto, Li Puma, Domenica Donatella, Grassi, Claudio, Palamara, A. T., De Chiara, G., Piacentini R. (ORCID:0000-0003-4215-1643), Li Puma D. D. (ORCID:0000-0001-6729-6967), Grassi C. (ORCID:0000-0001-7253-1685), Protto, V., Marcocci, M. E., Miteva, M. T., Piacentini, Roberto, Li Puma, Domenica Donatella, Grassi, Claudio, Palamara, A. T., De Chiara, G., Piacentini R. (ORCID:0000-0003-4215-1643), Li Puma D. D. (ORCID:0000-0001-6729-6967), and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: Herpes simplex virus-1 (HSV-1) is a ubiquitous DNA virus able to establish a life-long latent infection in host sensory ganglia. Following periodic reactivations, the neovirions usually target the site of primary infection causing recurrent diseases in susceptible individuals. However, reactivated HSV-1 may also reach the brain resulting in severe herpetic encephalitis or in asymptomatic infections. These have been reportedly linked to neurodegenerative disorders, such as Alzheimer's disease (AD), suggesting antiviral preventive or/therapeutic treatments as possible strategies to counteract AD onset and progression. Here, we provide an overview of the AD-like mechanisms driven by HSV-1-infection in neurons and discuss the ongoing trials repurposing anti-herpetic drugs to treat AD as well as preventive strategies aimed at blocking virus infection.
Published: 2022

19. Neural Stem Cell-Derived Extracellular Vesicles Counteract Insulin Resistance-Induced Senescence of Neurogenic Niche

Author: Natale, Francesca, Leone, Lucia, Rinaudo, Marco, Sollazzo, Raimondo, Barbati, Saviana Antonella, La Greca, Francesco, Spinelli, Matteo, Fusco, Salvatore, Grassi, Claudio, Natale F. (ORCID:0000-0001-7856-930X), Leone L. (ORCID:0000-0002-0695-7212), Rinaudo M. (ORCID:0000-0002-6130-7335), Sollazzo R., Barbati S. A. (ORCID:0000-0002-7574-0201), La Greca F., Spinelli M., Fusco S. (ORCID:0000-0003-3294-0016), Grassi C. (ORCID:0000-0001-7253-1685), Natale, Francesca, Leone, Lucia, Rinaudo, Marco, Sollazzo, Raimondo, Barbati, Saviana Antonella, La Greca, Francesco, Spinelli, Matteo, Fusco, Salvatore, Grassi, Claudio, Natale F. (ORCID:0000-0001-7856-930X), Leone L. (ORCID:0000-0002-0695-7212), Rinaudo M. (ORCID:0000-0002-6130-7335), Sollazzo R., Barbati S. A. (ORCID:0000-0002-7574-0201), La Greca F., Spinelli M., Fusco S. (ORCID:0000-0003-3294-0016), and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: Neural stem and progenitor cell (NSPC) depletion may play a crucial role in the cognitive impairment observed in many age-related non-communicable diseases. Insulin resistance affects brain functions through a plethora of mechanisms that remain poorly understood. In an experimental model of insulin resistant NSPCs, we identified a novel molecular circuit relying on insulin receptor substrate-1 (IRS-1)/ Forkhead box O (FoxO) signaling cascade and inhibiting the recruitment of transcription factors FoxO1 and FoxO3a on the promoters of genes regulating proliferation and self-renewal. Insulin resistance also epigenetically increased the expression of cyclin-dependent kinase inhibitor 1 (p21) and accelerated NSPC senescence. Of note, we found that stimulation of NSPCs with NSPC-derived exosomes (exo-NSPC) rescued IRS-1/FoxO activation and counteracted both the reduced proliferation and senescence of stem cells. Accordingly, intranasal administration of exo-NSPC counteracted the high-fat diet-dependent impairment of adult hippocampal neurogenesis in mice by restoring the balance between proliferating and senescent NSPCs in the hippocampus. Our findings suggest a novel mechanism underlying the metabolic control of NSPC fate potentially involved in the detrimental effects of metabolic disorders on brain plasticity. In addition, our data highlight the role of extracellular vesicle-mediated signals in the regulation of cell fate within the adult neurogenic niche.
Published: 2022

20. An unconventional mindset for shale gas surface facilities

Author: Guarnone, M., Rossi, F., Negri, E., Grassi, C., Genazzi, D., and Zennaro, R.
Published: 2012
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21. A 3D survey in archaeology. A comparison among software for image and range-based data integration

Author: Grassi C., Ronchi D., Ferdani D., Pocobelli G. F., and Manganelli Del Fà R.
Subjects: image-based software, arcaheological survey, UAS, photogrammetry, laserscanning
Abstract: This paper aims to evaluate the performance, in terms of accuracy and speed, of various commercial software (Reality Capture®, Agisoft Metashape®, and 3DF Zephyr®) for the integration among image and range-based survey data. Datasets were collected in the Nuragic complex called "la Prisgiona" (Arzachena, SS, Italy), during a collaborative research project between CNR - ISPC and the Municipality of Arzachena, using aerial photogrammetry, topographic, and TLS survey. Results of comparative tests demonstrate that, when working on architectural scale survey, passive and active techniques, are to be considered homologous in terms of accuracy and precision.
Published: 2022

22. Effect of plant density on Stevia rebaudiana Bertoni water consumption

Author: Pardini, A., Paterniani, M.L. Stipp, Baldi, A., Grassi, C., Giunta, R., and Mugnai, E.
Published: 2010

23. Can the Sympathetic Nervous System Activation Contribute to 'Context-Related' Modulations of the Stretch Reflex?

Author: Passatore, M., Grassi, C., Deriu, F., Ferrell, William R., editor, and Proske, Uwe, editor
Published: 1995
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24. Intravenous Ciprofloxacin for the Treatment of Lower Respiratory Tract Infections: Preliminary Results

Author: Mangiarotti, P., Grassi, C., and Garrard, Christopher, editor
Published: 1994
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25. Brain insulin resistance and hippocampal plasticity: mechanisms and biomarkers of cognitive decline.

Author: Spinelli, M., Fusco, S., Grassi, C., Spinelli M., Fusco S. (ORCID:0000-0003-3294-0016), Grassi C. (ORCID:0000-0001-7253-1685), Spinelli, M., Fusco, S., Grassi, C., Spinelli M., Fusco S. (ORCID:0000-0003-3294-0016), and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: In the last decade, much attention has been devoted to the effects of nutrient-related signals on brain development and cognitive functions. A turning point was the discovery that brain areas other than the hypothalamus expressed receptors for hormones related to metabolism. In particular, insulin signaling has been demonstrated to impact on molecular cascades underlying hippocampal plasticity, learning and memory. Here, we summarize the molecular evidence linking alteration of hippocampal insulin sensitivity with changes of both adult neurogenesis and synaptic plasticity. We also review the epidemiological studies and experimental models emphasizing the critical role of brain insulin resistance at the crossroad between metabolic and neurodegenerative disease. Finally, we brief novel findings suggesting how biomarkers of brain insulin resistance, involving the study of brain-derived extracellular vesicles and brain glucose metabolism, may predict the onset and/or the progression of cognitive decline.
Published: 2019

26. Enhancing plasticity mechanisms in the mouse motor cortex by anodal transcranial direct current stimulation: the contribution of nitric-oxide signaling.

Author: Barbati, Sa, Cocco, S, Longo, V, Spinelli, M, Gironi, K, Mattera, A, Paciello, F, Colussi, C, Podda, Mv, Grassi, C., Cocco S, Longo V, Spinelli M, Paciello F (ORCID:0000-0002-8473-8074), Podda MV (ORCID:0000-0002-2779-8417), Grassi C. (ORCID:0000-0001-7253-1685), Barbati, Sa, Cocco, S, Longo, V, Spinelli, M, Gironi, K, Mattera, A, Paciello, F, Colussi, C, Podda, Mv, Grassi, C., Cocco S, Longo V, Spinelli M, Paciello F (ORCID:0000-0002-8473-8074), Podda MV (ORCID:0000-0002-2779-8417), and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: Consistent body of evidence shows that transcranial direct-current stimulation (tDCS) over the primary motor cortex (M1) facilitates motor learning and promotes recovery after stroke. However, the knowledge of molecular mechanisms behind tDCS effects needs to be deepened for a more rational use of this technique in clinical settings. Here we characterized the effects of anodal tDCS of M1, focusing on its impact on glutamatergic synaptic transmission and plasticity. Mice subjected to tDCS displayed increased long-term potentiation (LTP) and enhanced basal synaptic transmission at layer II/III horizontal connections. They performed better than sham-stimulated mice in the single-pellet reaching task and exhibited increased forelimb strength. Dendritic spine density of layer II/III pyramidal neurons was also increased by tDCS. At molecular level, tDCS enhanced: 1) BDNF expression, 2) phosphorylation of CREB, CaMKII, and GluA1, and 3) S-nitrosylation of GluA1 and HDAC2. Blockade of nitric oxide synthesis by L-NAME prevented the tDCS-induced enhancement of GluA1 phosphorylation at Ser831 and BDNF levels, as well as of miniature excitatory postsynaptic current (mEPSC) frequency, LTP and reaching performance. Collectively, these findings demonstrate that anodal tDCS engages plasticity mechanisms in the M1 and highlight a role for nitric oxide (NO) as a novel mediator of tDCS effects.
Published: 2019

27. Maternal insulin resistance multigenerationally impairs synaptic plasticity and memory via gametic mechanisms.

Author: Fusco, S, Spinelli, M, Cocco, S, Ripoli, C, Mastrodonato, A, Natale, F, Rinaudo, M, Livrizzi, G, Grassi, C., Fusco S (ORCID:0000-0003-3294-0016), Spinelli M, Cocco S, Ripoli C (ORCID:0000-0002-5315-0163), Natale F (ORCID:0000-0001-7856-930X), Rinaudo M (ORCID:0000-0002-6130-7335), Grassi C. (ORCID:0000-0001-7253-1685), Fusco, S, Spinelli, M, Cocco, S, Ripoli, C, Mastrodonato, A, Natale, F, Rinaudo, M, Livrizzi, G, Grassi, C., Fusco S (ORCID:0000-0003-3294-0016), Spinelli M, Cocco S, Ripoli C (ORCID:0000-0002-5315-0163), Natale F (ORCID:0000-0001-7856-930X), Rinaudo M (ORCID:0000-0002-6130-7335), and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: Metabolic diseases harm brain health and cognitive functions, but whether maternal metabolic unbalance may affect brain plasticity of next generations is still unclear. Here, we demonstrate that maternal high fat diet (HFD)-dependent insulin resistance multigenerationally impairs synaptic plasticity, learning and memory. HFD downregulates BDNF and insulin signaling in maternal tissues and epigenetically inhibits BDNF expression in both germline and hippocampus of progeny. Notably, exposure of the HFD offspring to novel enriched environment restores Bdnf epigenetic activation in the male germline and counteracts the transmission of cognitive impairment to the next generations. BDNF administration to HFD-fed mothers or preserved insulin sensitivity in HFD-fed p66Shc KO mice also prevents the intergenerational transmission of brain damage to the progeny. Collectively, our data suggest that maternal diet multigenerationally impacts on descendants' brain health via gametic mechanisms susceptible to lifestyle.
Published: 2019

28. Anti-oxidant and anti-inflammatory effects of caffeic acid: in vivo evidences in a model of noise-induced hearing loss

Author: Paciello F, Di Pino A, Rolesi R, Troiani D, Paludetti G, Grassi C, Fetoni AR, Paciello, F, Di Pino, A, Rolesi, R, Troiani, D, Paludetti, G, Grassi, C, and Fetoni, Ar
Published: 2020

29. The dual role of curcumin and ferulic acid in counteracting chemoresistance and cisplatin-induced ototoxicity

Author: Paciello F, Fetoni AR, Mezzogori D, Rolesi R, Di Pino A, Paludetti G, Grassi C, Troiani D, Paciello, F, Fetoni, Ar, Mezzogori, D, Rolesi, R, Di Pino, A, Paludetti, G, Grassi, C, and Troiani, D
Published: 2020

30. Somato-Vegetative Interaction at the Peripheral Level: Possible Effects on Motor Performance

Author: Passatore, M., Grassi, C., Koepchen, Hans-Peter, editor, and Huopaniemi, Timo, editor
Published: 1991
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31. P.077 Mixed autoimmune hemolytic anemia: an unusual cause of ischemic stroke and extensive cerebral microbleeds

Author: Wan, M, primary, Ganesh, A, additional, Grassi, C, additional, and Demchuk, A, additional
Published: 2021
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32. Sympathetically-Induced Changes in the Spindle Afferent Response to Vibratory Stimuli Eliciting the TVR in Rabbit Jaw Closing Muscles

Author: Passatore, M., Deriu, F., Roatta, S., Grassi, C., Taylor, Anthony, editor, Gladden, Margaret H., editor, and Durbaba, Rade, editor
Published: 1995
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33. Noise-induced cochlear damage involves ppar down-regulation through the interplay between oxidative stress and inflammation

Author: Paciello, F. (ORCID:0000-0002-8473-8074), Pisani, A., Rolesi, R., Escarrat, V., Galli, J. (ORCID:0000-0001-6353-6249), Paludetti, G. (ORCID:0000-0003-2480-1243), Grassi, C. (ORCID:0000-0001-7253-1685), Troiani, D. (ORCID:0000-0002-5665-7410), Fetoni, A. R. (ORCID:0000-0001-5405-4301), Paciello, F. (ORCID:0000-0002-8473-8074), Pisani, A., Rolesi, R., Escarrat, V., Galli, J. (ORCID:0000-0001-6353-6249), Paludetti, G. (ORCID:0000-0003-2480-1243), Grassi, C. (ORCID:0000-0001-7253-1685), Troiani, D. (ORCID:0000-0002-5665-7410), and Fetoni, A. R. (ORCID:0000-0001-5405-4301)
Abstract: The cross-talk between oxidative stress and inflammation seems to play a key role in noise-induced hearing loss. Several studies have addressed the role of PPAR receptors in mediating antioxidant and anti-inflammatory effects and, although its protective activity has been demonstrated in several tissues, less is known about how PPARs could be involved in cochlear dysfunction induced by noise exposure. In this study, we used an in vivo model of noise-induced hearing loss to investigate how oxidative stress and inflammation participate in cochlear dysfunction through PPAR signaling pathways. Specifically, we found a progressive decrease in PPAR expression in the cochlea after acoustic trauma, paralleled by an increase in oxidative stress and inflammation. By comparing an antioxidant (Q-ter) and an anti-inflammatory (Anakinra) treatment, we demonstrated that oxidative stress is the primary element of damage in noise-induced cochlear injury and that increased inflammation can be considered a consequence of PPAR down-regulation induced by ROS production. Indeed, by decreasing oxidative stress, PPARs returned to control values, reactivating the negative control on inflammation in a feedback loop.
Published: 2021

34. Epigenetic regulation of neural stem cells: The emerging role of nucleoporins

Author: Colussi, C., Grassi, Claudio, Grassi C. (ORCID:0000-0001-7253-1685), Colussi, C., Grassi, Claudio, and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: Nucleoporins (Nups) are components of the nuclear pore complex that, besides regulating nucleus-cytoplasmic transport, emerged as a hub for chromatin interaction and gene expression modulation. Specifically, Nups act in a dynamic manner both at specific gene level and in the topological organization of chromatin domains. As such, they play a fundamental role during development and determination of stemness/differentiation balance in stem cells. An increasing number of reports indicate the implication of Nups in many central nervous system functions with great impact on neurogenesis, neurophysiology, and neurological disorders. Nevertheless, the role of Nup-mediated epigenetic regulation in embryonic and adult neural stem cells (NSCs) is a field largely unexplored and the comprehension of their mechanisms of action is only beginning to be unveiled. After a brief overview of epigenetic mechanisms, we will present and discuss the emerging role of Nups as new effectors of neuroepigenetics and as dynamic platform for chromatin function with specific reference to the biology of NSCs.
Published: 2021

35. Metabolic reprogramming by malat1 depletion in prostate cancer

Author: Nanni, Simona, Aiello, A., Salis, Chiara, Re, Agnese, Cencioni, C., Bacci, Lorenza, Pierconti, Francesco, Pinto, Francesco, Ripoli, Cristian, Ostano, P., Baroni, Silvia, Lazzarino, Giacomo, Tavazzi, Barbara, Pugliese, D., Bassi, Pierfrancesco, Grassi, Claudio, Panunzi, Simona, Chiorino, G., Pontecorvi, Alfredo, Gaetano, C., Farsetti, A., Nanni S. (ORCID:0000-0002-3320-1584), Salis C., Re A., Bacci L., Pierconti F. (ORCID:0000-0003-0951-4131), Pinto F., Ripoli C. (ORCID:0000-0002-5315-0163), Baroni S. (ORCID:0000-0002-3410-2617), Lazzarino G., Tavazzi B. (ORCID:0000-0001-8743-0895), Bassi P. (ORCID:0000-0002-4313-8427), Grassi C. (ORCID:0000-0001-7253-1685), Panunzi S., Pontecorvi A. (ORCID:0000-0003-0570-6865), Nanni, Simona, Aiello, A., Salis, Chiara, Re, Agnese, Cencioni, C., Bacci, Lorenza, Pierconti, Francesco, Pinto, Francesco, Ripoli, Cristian, Ostano, P., Baroni, Silvia, Lazzarino, Giacomo, Tavazzi, Barbara, Pugliese, D., Bassi, Pierfrancesco, Grassi, Claudio, Panunzi, Simona, Chiorino, G., Pontecorvi, Alfredo, Gaetano, C., Farsetti, A., Nanni S. (ORCID:0000-0002-3320-1584), Salis C., Re A., Bacci L., Pierconti F. (ORCID:0000-0003-0951-4131), Pinto F., Ripoli C. (ORCID:0000-0002-5315-0163), Baroni S. (ORCID:0000-0002-3410-2617), Lazzarino G., Tavazzi B. (ORCID:0000-0001-8743-0895), Bassi P. (ORCID:0000-0002-4313-8427), Grassi C. (ORCID:0000-0001-7253-1685), Panunzi S., and Pontecorvi A. (ORCID:0000-0003-0570-6865)
Abstract: The lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) promotes growth and progression in prostate cancer (PCa); however, little is known about its possible impact in PCa metabolism. The aim of this work has been the assessment of the metabolic reprogramming associated with MALAT1 silencing in human PCa cells and in an ex vivo model of organotypic slice cultures (OSCs). Cultured cells and OSCs derived from primary tumors were transfected with MALAT1 specific gapmers. Cell growth and survival, gene profiling, and evaluation of targeted metabolites and metabolic enzymes were assessed. Computational analysis was made considering expression changes occurring in metabolic markers following MALAT1 targeting in cultured OSCs. MALAT1 silencing reduced expression of some metabolic enzymes, including malic enzyme 3, pyruvate dehydrogenase kinases 1 and 3, and choline kinase A. Consequently, PCa metabolism switched toward a glycolytic phenotype characterized by increased lactate production paralleled by growth arrest and cell death. Conversely, the function of mitochondrial succinate dehydrogenase and the expression of oxidative phosphorylation enzymes were markedly reduced. A similar effect was observed in OSCs. Based on this, a predictive algorithm was developed aimed to predict tumor recurrence in a subset of patients. MALAT1 targeting by gapmer delivery restored normal metabolic energy pathway in PCa cells and OSCs.
Published: 2021

36. High-fat diet leads to reduced protein o-glcnacylation and mitochondrial defects promoting the development of alzheimer’s disease signatures

Author: Zuliani, I., Lanzillotta, C., Tramutola, A., Barone, E., Perluigi, M., Rinaldo, S., Paone, A., Cutruzzola, F., Bellanti, F., Spinelli, M., Natale, Francesca, Fusco, Salvatore, Grassi, Claudio, Di Domenico, F., Natale F. (ORCID:0000-0001-7856-930X), Fusco S. (ORCID:0000-0003-3294-0016), Grassi C. (ORCID:0000-0001-7253-1685), Zuliani, I., Lanzillotta, C., Tramutola, A., Barone, E., Perluigi, M., Rinaldo, S., Paone, A., Cutruzzola, F., Bellanti, F., Spinelli, M., Natale, Francesca, Fusco, Salvatore, Grassi, Claudio, Di Domenico, F., Natale F. (ORCID:0000-0001-7856-930X), Fusco S. (ORCID:0000-0003-3294-0016), and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: The disturbance of protein O-GlcNAcylation is emerging as a possible link between altered brain metabolism and the progression of neurodegeneration. As observed in brains with Alzheimer’s disease (AD), flaws of the cerebral glucose uptake translate into reduced protein O-GlcNAcylation, which promote the formation of pathological hallmarks. A high-fat diet (HFD) is known to foster metabolic dysregulation and insulin resistance in the brain and such effects have been associated with the reduction of cognitive performances. Remarkably, a significant role in HFD-related cognitive decline might be played by aberrant protein O-GlcNAcylation by triggering the development of AD signature and mitochondrial impairment. Our data support the impairment of total protein O-GlcNAcylation profile both in the brain of mice subjected to a 6-week high-fat-diet (HFD) and in our in vitro transposition on SH-SY5Y cells. The reduction of protein O-GlcNAcylation was associated with the development of insulin resistance, induced by overfeeding (i.e., defective insulin signaling and reduced mitochondrial activity), which promoted the dysregulation of the hexosamine biosynthetic pathway (HBP) flux, through the AMPK-driven reduction of GFAT1 activation. Further, we observed that a HFD induced the selective impairment of O-GlcNAcylated-tau and of O-GlcNAcylated-Complex I subunit NDUFB8, thus resulting in tau toxicity and reduced respiratory chain functionality respectively, highlighting the involvement of this posttranslational modification in the neurodegenerative process.
Published: 2021

37. Welfare assessment of buffalo cows: use of expert opinion elicitation for the characterization of hazards, welfare promoters and animal-based measures

Author: VECCHIO D., ROSSI P., GRASSI C., RUSSO P., IEMMA L., CORONATI D., PALLADIO M. CONSALVO F., DE ROSA Giuseppe, NAPOLITANO F., NEGLIA Gianluca, DE CARLO E., LORENZI V., FUSI Francesca, BOLZONI L., PONGOLIN S., GALIERO G., ALBORAL G. L., BERTOCCH L., Soysal M.I., Vecchio, D., Rossi, P., Grassi, C., Russo, P., Iemma, L., Coronati, D., Palladio, M. CONSALVO F., DE ROSA, Giuseppe, Napolitano, F., Neglia, Gianluca, DE CARLO, E., Lorenzi, V., Fusi, Francesca, Bolzoni, L., Pongolin, S., Galiero, G., Alboral, G. L., and Bertocch, L.
Subjects: buffalo welfare, expert opinion
Abstract: An expert opinion elicitation, based on a modified Delphi technique, was organized to collect the opinion of 14 Italian veterinarians with the aim of conducting a hazard and a welfare promoter characterization for defining and weighing a list of management and housing factors potentially associated with negative or positive welfare outcomes in buffalo kept in loose housing systems. In addition, the 14 experts judged a set of animal-based measures (ABMs), rating their appropriateness and the level of animal pain and suffering due to the welfare consequences they measure. Experts were asked to score 52 hazards, 45 welfare promoters and 14 ABMs. Management and housing hazards, that were determined to be associated with a very high negative impact on the welfare of buffalo, were: lack of water availability, uncorrected animal grouping strategy, dirty and unmanaged lying area, incorrect milking routine, poor maintenance of the milking system, lack of litter for calves, insufficient shaded lying area and/or lack of any cooling system and presence of inadequate or slippery floor in walking areas. Management and housing welfare promoters dealing with optimal buffalo comfort around resting obtained the highest ratings, in particular: housing free for all animals and the possibility for adult buffaloes to access a loafing area of at least 7 m²and/or to a pasture for at least 60 days a year; cleanleness of the calf boxes, a hospital pen of at least 8 m²/parturient animal. In addition, high scores were given to appropriate grouping, adequate temperature, humidity and ventilation, presence of experienced and trained stockpersons. As to ABMs, the highest rankings were given to mortality of calves and adult buffaloes, percentage of animals showing vaginal and/or uterine prolapses, body condition scoring and percentage of deformed claws in adult buffaloes. Our results represent the starting point for the development of the first protocol for the assessment of the welfare of buffalo farmed in loose housing systems.
Published: 2019

38. Publisher Correction: The dual role of curcumin and ferulic acid in counteracting chemoresistance and cisplatin-induced ototoxicity (Scientific Reports, (2020), 10, 1, (1063), 10.1038/s41598-020-57965-0)

Author: Paciello, F. (ORCID:0000-0002-8473-8074), Fetoni, A. R. (ORCID:0000-0001-5405-4301), Mezzogori, D., Rolesi, R., Di Pino, A., Paludetti, G. (ORCID:0000-0003-2480-1243), Grassi, C. (ORCID:0000-0001-7253-1685), Troiani, D. (ORCID:0000-0002-5665-7410), Paciello, F. (ORCID:0000-0002-8473-8074), Fetoni, A. R. (ORCID:0000-0001-5405-4301), Mezzogori, D., Rolesi, R., Di Pino, A., Paludetti, G. (ORCID:0000-0003-2480-1243), Grassi, C. (ORCID:0000-0001-7253-1685), and Troiani, D. (ORCID:0000-0002-5665-7410)
Abstract: In the original version of this Article, the author Anna Rita Fetoni was incorrectly indexed. This error has now been corrected.
Published: 2020

39. Herpes Simplex Virus-1 in the Brain: The Dark Side of a Sneaky Infection

Author: Marcocci, M. E., Napoletani, G., Protto, V., Kolesova, O., Piacentini, Roberto, Li Puma, Domenica Donatella, Lomonte, P., Grassi, Claudio, Palamara, A. T., De Chiara, G., Piacentini R. (ORCID:0000-0003-4215-1643), Li Puma D. D. (ORCID:0000-0001-6729-6967), Grassi C. (ORCID:0000-0001-7253-1685), Marcocci, M. E., Napoletani, G., Protto, V., Kolesova, O., Piacentini, Roberto, Li Puma, Domenica Donatella, Lomonte, P., Grassi, Claudio, Palamara, A. T., De Chiara, G., Piacentini R. (ORCID:0000-0003-4215-1643), Li Puma D. D. (ORCID:0000-0001-6729-6967), and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: Herpes simplex virus-1 (HSV-1) establishes latency preferentially in sensory neurons of peripheral ganglia. A variety of stresses can induce recurrent reactivations of the virus, which spreads and then actively replicates to the site of primary infection (usually the lips or eyes). Viral particles produced following reactivation can also reach the brain, causing a rare but severe form of diffuse acute infection, namely herpes simplex encephalitis. Most of the time, this infection is clinically asymptomatic. However, it was recently correlated with the production and accumulation of neuropathological biomarkers of Alzheimer's disease. In this review we discuss the different cellular and molecular mechanisms underlying the acute and long-term damage caused by HSV-1 infection in the brain.
Published: 2020

40. Combined molecular and mathematical analysis of long noncoding RNAs expression in fine needle aspiration biopsies as novel tool for early diagnosis of thyroid cancer

Author: Possieri, C., Locantore, Pietro, Salis, Chiara, Bacci, Lorenza, Aiello, Antimo, Fadda, Guido, De Crea, Carmela, Raffaelli, Marco, Bellantone, Rocco Domenico Alfonso, Grassi, Claudio, Strigari, L., Farsetti, Antonella, Pontecorvi, Alfredo, Nanni, Simona, Locantore P., Salis C., Bacci L., Aiello A., Fadda G. (ORCID:0000-0003-2013-7293), De Crea C. (ORCID:0000-0002-7303-9657), Raffaelli M. (ORCID:0000-0002-1259-2491), Bellantone R. (ORCID:0000-0002-0844-3469), Grassi C. (ORCID:0000-0001-7253-1685), Farsetti A., Pontecorvi A. (ORCID:0000-0003-0570-6865), Nanni S. (ORCID:0000-0002-3320-1584), Possieri, C., Locantore, Pietro, Salis, Chiara, Bacci, Lorenza, Aiello, Antimo, Fadda, Guido, De Crea, Carmela, Raffaelli, Marco, Bellantone, Rocco Domenico Alfonso, Grassi, Claudio, Strigari, L., Farsetti, Antonella, Pontecorvi, Alfredo, Nanni, Simona, Locantore P., Salis C., Bacci L., Aiello A., Fadda G. (ORCID:0000-0003-2013-7293), De Crea C. (ORCID:0000-0002-7303-9657), Raffaelli M. (ORCID:0000-0002-1259-2491), Bellantone R. (ORCID:0000-0002-0844-3469), Grassi C. (ORCID:0000-0001-7253-1685), Farsetti A., Pontecorvi A. (ORCID:0000-0003-0570-6865), and Nanni S. (ORCID:0000-0002-3320-1584)
Abstract: Purpose: In presence of indeterminate lesions by fine needle aspiration (FNA), thyroid cancer cannot always be easily diagnosed by conventional cytology. As a consequence, unnecessary removal of thyroid gland is performed in patients without cancer based on the lack of optimized diagnostic criteria. Aim of this study is identifying a molecular profile based on long noncoding RNAs (lncRNAs) expression capable to discriminate between benign and malignant nodules. Methods: Patients were subjected to surgery (n = 19) for cytologic suspicious thyroid nodules or to FNA biopsy (n = 135) for thyroid nodules suspicious at ultrasound. Three thyroid-specific genes (TG, TPO, and NIS), six cancer-associated lncRNAs (MALAT1, NEAT1, HOTAIR, H19, PVT1, MEG3), and two housekeeping genes (GAPDH and P0) were analyzed using Droplet Digital PCR (ddPCR). Results: Based on higher co-expression in malignant (n = 11) but not in benign (n = 8) nodules after surgery, MALAT1, PVT1 and HOTAIR were selected as putative cancer biomarkers to analyze 135 FNA samples. Cytological and histopathological data from a subset of FNA patients (n = 34) were used to define a predictive algorithm based on a Naïve Bayes classifier using co-expression of MALAT1, PVT1, HOTAIR, and cytological class. This classifier exhibited a significant separation capability between malignant and benign nodules (P < 0.0001) as well as both rule in and rule out test potential with an accuracy of 94.12% and a negative predictive value (NPV) of 100% and a positive predictive value (PPV) of 91.67%. Conclusions: ddPCR analysis of selected lncRNAs in FNA biopsies appears a suitable molecular tool with the potential of improving diagnostic accuracy.
Published: 2020

41. Somatic deletion in exon 10 of aryl hydrocarbon receptor gene in human GH-secreting pituitary tumors

Author: Re, Agnese, Ferraù, F, Cafiero, C, Spagnolo, F, Barresi, V, Romeo, Dp, Ragonese, Mauro, Grassi, Claudio, Pontecorvi, Alfredo, Farsetti, A, Cannavò, S, Re A, Ragonese M, Grassi C (ORCID:0000-0001-7253-1685), Pontecorvi A (ORCID:0000-0003-0570-6865), Re, Agnese, Ferraù, F, Cafiero, C, Spagnolo, F, Barresi, V, Romeo, Dp, Ragonese, Mauro, Grassi, Claudio, Pontecorvi, Alfredo, Farsetti, A, Cannavò, S, Re A, Ragonese M, Grassi C (ORCID:0000-0001-7253-1685), and Pontecorvi A (ORCID:0000-0003-0570-6865)
Abstract: Objective/Purpose: The aryl hydrocarbon receptor (AHR) pathway plays a critical role in the biology of Growth Hormone (GH)-secreting pituitary tumor (somatotropinoma). Germline rs2066853 AHR variant was found to be more frequent among acromegaly patients and associated with a more severe disease with larger invasive somatropinoma, and with resistance to somatostatin analogs treatment in patients living in polluted areas. However, no somatic changes in AHR gene have been reported so far in acromegaly patients. On that basis, the aim of the study was to assess at the somatic level the AHR gene status encompassing exon 10 region, also because of the high rate of variants found in this genomic region. Methods: A cohort of 13 patients aged 20–76 years with biochemical, clinical and histological diagnosis of somatotropinoma was studied. DNA and RNA from pituitary tumor histological samples have been extracted and analyzed by PCR and direct sequencing for AHR gene variants, and compared with corresponding patients’ germline DNA as well as normal pituitary tissue as reference control. Results: A degenerated letter codes in the region corresponding to AHR exon 10 (c.1239-c.2056) was detected in somatotropinomas-derived DNA but not in that of matched germline and pituitary normal tissue. By multiple PCR and sequencing analysis, we observed amplification only before codon 1246 and after codon 1254, confirming the presence of a tumor-restricted somatic deletion in the 5’ upstream region of AHR exon 10. Analysis of PCR-amplified cDNA revealed a wildtype sequence of exon 9 and 10 in normal pituitary tissue, and a wildtype sequence of exon 9 and 10 up to codon 1246 and no sequence after the deletion region (c.1246-c.1254) in 6 out of 9 tumor samples. Patients carrying the germline rs2066853 AHR variant showed no somatic LOH at the corresponding genetic locus. Conclusion: This is the first demonstration of a recurrent somatic deletion in the exon 10 of the AHR gene in somatotropino
Published: 2020

42. Neural stem cell‐derived exosomes revert HFD-dependent memory impairment via CREB‐BDNF signalling

Author: Spinelli, Matteo, Natale, Francesca, Rinaudo, Marco, Leone, Lucia, Mezzogori, D, Fusco, Salvatore, Grassi, Claudio, Spinelli M, Natale F (ORCID:0000-0001-7856-930X), Rinaudo M (ORCID:0000-0002-6130-7335), Leone L (ORCID:0000-0002-0695-7212), Fusco S (ORCID:0000-0003-3294-0016), Grassi C (ORCID:0000-0001-7253-1685), Spinelli, Matteo, Natale, Francesca, Rinaudo, Marco, Leone, Lucia, Mezzogori, D, Fusco, Salvatore, Grassi, Claudio, Spinelli M, Natale F (ORCID:0000-0001-7856-930X), Rinaudo M (ORCID:0000-0002-6130-7335), Leone L (ORCID:0000-0002-0695-7212), Fusco S (ORCID:0000-0003-3294-0016), and Grassi C (ORCID:0000-0001-7253-1685)
Abstract: Overnutrition and metabolic disorders impair cognitive functions through molecular mechanisms still poorly understood. In mice fed with a high fat diet (HFD) we analysed the expression of synaptic plasticity‐related genes and the activation of cAMP response elementbinding protein (CREB)‐brain‐derived neurotrophic factor (BDNF)‐tropomyosin receptor kinase B (TrkB) signalling. We found that a HFD inhibited both CREB phosphorylation and the expression of a set of CREB target genes in the hippocampus. The intranasal administration of neural stem cell (NSC)‐derived exosomes (exo‐NSC) epigenetically restored the transcription of Bdnf, nNOS, Sirt1, Egr3, and RelA genes by inducing the recruitment of CREB on their regulatory sequences. Finally, exo‐NSC administration rescued both BDNF signalling and memory in HFD mice. Collectively, our findings highlight novel mechanisms underlying HFD‐related memory impairment and provide evidence of the potential therapeutic effect of exo‐NSC against metabolic disease‐related cognitive decline
Published: 2020

43. Plasma BDNF levels following transcranial direct current stimulation allow prediction of synaptic plasticity and memory deficits in 3×Tg-AD mice.

Author: Cocco, Sara, Rinaudo, Marco, Fusco, Salvatore, Longo, Valentina, Gironi, Katia, Renna, Pietro, Aceto, Giuseppe, Mastrodonato, Alessia, Li Puma, Domenica Donatella, Podda, Maria Vittoria, Grassi, Claudio, Cocco S., Rinaudo M. (ORCID:0000-0002-6130-7335), Fusco S. (ORCID:0000-0003-3294-0016), Longo V., Gironi K., Renna P., Aceto G., Mastrodonato A., Li Puma. (ORCID:0000-0001-6729-6967), Podda (ORCID:0000-0002-2779-8417), Grassi C. (ORCID:0000-0001-7253-1685), Cocco, Sara, Rinaudo, Marco, Fusco, Salvatore, Longo, Valentina, Gironi, Katia, Renna, Pietro, Aceto, Giuseppe, Mastrodonato, Alessia, Li Puma, Domenica Donatella, Podda, Maria Vittoria, Grassi, Claudio, Cocco S., Rinaudo M. (ORCID:0000-0002-6130-7335), Fusco S. (ORCID:0000-0003-3294-0016), Longo V., Gironi K., Renna P., Aceto G., Mastrodonato A., Li Puma. (ORCID:0000-0001-6729-6967), Podda (ORCID:0000-0002-2779-8417), and Grassi C. (ORCID:0000-0001-7253-1685)
Abstract: Early diagnosis of Alzheimer’s disease (AD) supposedly increases the effectiveness of therapeutic interventions. However, presently available diagnostic procedures are either invasive or require complex and expensive technologies, which cannot be applied at a larger scale to screen populations at risk of AD.We were looking for a biomarker allowing to unveil a dysfunction of molecular mechanisms, which underly synaptic plasticity and memory, before the AD phenotype is manifested and investigated the effects of transcranial direct current stimulation (tDCS) in 3 x Tg-AD mice, an experimental model of AD which does not exhibit any long-term potentiation (LTP) and memory deficits at the age of 3 months (3 x Tg-AD-3M). Our results demonstrated that tDCS differentially affected 3 x Tg-AD-3M and age-matched wild-type (WT) mice. While tDCS increased LTP at CA3-CA1 synapses and memory in WT mice, it failed to elicit these effects in 3 x Tg-AD-3M mice. Remarkably, 3 x Tg-AD-3M mice did not show the tDCS-dependent increases in pCREBSer133 and pCaMKIIThr286, which were found in WT mice. Of relevance, tDCS induced a significant increase of plasma BDNF levels in WT mice, which was not found in 3 x Tg-AD-3M mice. Collectively, our results showed that plasticity mechanisms are resistant to tDCS effects in the pre-AD stage. In particular, the lack of BDNF responsiveness to tDCS in 3 x Tg-AD-3M mice suggests that combining tDCS with dosages of plasma BDNF levels may provide an easy-todetect and low-cost biomarker of covert impairment of synaptic plasticity mechanisms underlying memory, which could be clinically applicable. Testing proposed here might be useful to identify AD in its preclinical stage, allowing timely and, hopefully, more effective disease-modifying interventions.
Published: 2020

44. NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl‐glutathione‐coated gold nanoparticles

Author: Buonerba, A, Lapenta, R, Donniacuo, A, Licasale, M, Vezzoli, E, Milione, S, Capacchione, C, Tecce, Mf, Falqui, A, Piacentini, Roberto, Grassi, Claudio, Grassi, A, Piacentini R (ORCID:0000-0003-4215-1643), Grassi C (ORCID:0000-0001-7253-1685), Buonerba, A, Lapenta, R, Donniacuo, A, Licasale, M, Vezzoli, E, Milione, S, Capacchione, C, Tecce, Mf, Falqui, A, Piacentini, Roberto, Grassi, Claudio, Grassi, A, Piacentini R (ORCID:0000-0003-4215-1643), and Grassi C (ORCID:0000-0001-7253-1685)
Abstract: Theranostics based on two-photon excitation of therapeutics in the NIR region is an emerging and powerful tool in cancer therapy since this radiation deeply penetrates healthy biological tissues and produces selective cell death. Aggregates of gold nanoparticles coated with glutathione corona functionalized with the dansyl chromophore (a-DG-AuNPs) were synthesized and found efficient nanodevice for applications in photothermal therapy (PTT). Actually the nanoparticle aggregation enhances the quenching of radiative excitation and the consequent conversion into heat. The a-DG-AuNPs are readily internalized in Hep G2 where the chromophore acts as both antenna and transducer of the NIR radiation under two-photons excitation, determining efficient cell ablation via photothermal effect
Published: 2020

45. Glucose Overload Inhibits Glutamatergic Synaptic Transmission: A Novel Role for CREB-Mediated Regulation of Synaptotagmins 2 and 4

Author: Ripoli, Cristian, Spinelli, Matteo, Natale, Francesca, Fusco, Salvatore, Grassi, Claudio, Ripoli C (ORCID:0000-0002-5315-0163), Spinelli M, Natale F (ORCID:0000-0001-7856-930X), Fusco S (ORCID:0000-0003-3294-0016), Grassi C (ORCID:0000-0001-7253-1685), Ripoli, Cristian, Spinelli, Matteo, Natale, Francesca, Fusco, Salvatore, Grassi, Claudio, Ripoli C (ORCID:0000-0002-5315-0163), Spinelli M, Natale F (ORCID:0000-0001-7856-930X), Fusco S (ORCID:0000-0003-3294-0016), and Grassi C (ORCID:0000-0001-7253-1685)
Abstract: Glucose metabolism derangement is critically involved in the age-related memory loss but the underlying molecular mechanisms are still poorly understood. In a mouse model of type 1 diabetes we found memory impairment associated with inhibition of the transcription factor CREB and alteration of pre- and post-synaptic protein expression in the hippocampus. Accordingly, glucose excess negatively affected activity-dependent CREB phosphorylation and CREB-mediated mRNA expression of synaptic proteins in hippocampal primary neurons. Specifically, glucose excess inhibited the activity-dependent recruitment of CREB on the regulatory sequences of synaptotagmin (SYT) 2 and 4 promoters and the expression of SYT4 protein. As a result, high glucose affected both the frequency of miniature excitatory postsynaptic currents and NMDA receptor-mediated currents in autaptic hippocampal neuronal cultures. Collectively, our findings highlight novel mechanisms underlying hyperglycaemia-related memory loss, including CREB-dependent downregulation of synaptotagmin expression
Published: 2020

46. Brain insulin resistance impairs hippocampal plasticity

Author: Spinelli, Matteo, Fusco, Salvatore, Grassi, Claudio, Spinelli M., Fusco S. (ORCID:0000-0003-3294-0016), Grassi C (ORCID:0000-0001-7253-1685), Spinelli, Matteo, Fusco, Salvatore, Grassi, Claudio, Spinelli M., Fusco S. (ORCID:0000-0003-3294-0016), and Grassi C (ORCID:0000-0001-7253-1685)
Abstract: Nutrient-related signals have been demonstrated to influence brain development and cognitive functions. In particular, insulin signaling has been shown to impact on molecular cascades underlying hippocampal plasticity, learning and memory. Alteration of brain insulin signaling interferes with the maintenance of neural stem cell niche and neuronal activity in the hippocampus. Brain insulin resistance is also emerging as key factor causing the cognitive impairment observed in metabolic and neurodegenerative diseases. Here, we review the molecular mechanisms involved in the insulin modulation of both adult neurogenesis and synaptic activity in the hippocampus. We also summarize the effects of altered insulin sensitivity on hippocampal plasticity. Finally, we reassume the experimental and epidemiological evidence highlighting the critical role of brain insulin resistance at the crossroad between type 2 diabetes and Alzheimer's disease
Published: 2020

47. A Case Study of Malignant Edema in Postpartum Mediterranean Buffaloes (Bubalus bubalis)

Author: D'Angelo, L., Martucciello, A., Marianelli, C., Grassi, C., Schiavo, L., Armas, F., Alfano, D., Vecchio, D., Carlo, E. De, D'Angelo, L., Martucciello, A., Marianelli, C., Grassi, C., Schiavo, L., Armas, F., Alfano, D., Vecchio, D., and Carlo, E. De
Abstract: The paper describes for the first time four cases of postparturient malignant edema in water buffaloes due to Clostridium septicum (Cl. septicum). The study was carried out on four primiparous buffaloes that showed swelling of perineal and perivulvar areas, fever and agalactia a few hours after calving. Two of them died within 20 hours after calving. The other two developed edema in the skeletal muscles of one leg and were treated with sulfadiazine-trimethoprim for 10 days. The clinical signs completely resolved. Culture and molecular investigations identified the pathogen isolated from exudate taken from the vulva as Cl. septicum. The isolate showed multi-drug resistance. In the management of infection due to Cl. septicum, timely diagnosis and the chirurgic curettage, associated with a broad-spectrum antibiotic therapy, were found to be sufficient for the survival and recovery of the infected animals.
Published: 2020

48. Publisher Correction: The dual role of curcumin and ferulic acid in counteracting chemoresistance and cisplatin-induced ototoxicity (Scientific Reports, (2020), 10, 1, (1063), 10.1038/s41598-020-57965-0)

Author: Paciello, Fabiola, Fetoni, Anna Rita, Mezzogori, D., Rolesi, Rolando, Di Pino, Antonella, Paludetti, Gaetano, Grassi, Claudio, Troiani, Diana, Paciello F. (ORCID:0000-0002-8473-8074), Fetoni A. R. (ORCID:0000-0001-5405-4301), Rolesi R., Di Pino A., Paludetti G. (ORCID:0000-0003-2480-1243), Grassi C. (ORCID:0000-0001-7253-1685), Troiani D. (ORCID:0000-0002-5665-7410), Paciello, Fabiola, Fetoni, Anna Rita, Mezzogori, D., Rolesi, Rolando, Di Pino, Antonella, Paludetti, Gaetano, Grassi, Claudio, Troiani, Diana, Paciello F. (ORCID:0000-0002-8473-8074), Fetoni A. R. (ORCID:0000-0001-5405-4301), Rolesi R., Di Pino A., Paludetti G. (ORCID:0000-0003-2480-1243), Grassi C. (ORCID:0000-0001-7253-1685), and Troiani D. (ORCID:0000-0002-5665-7410)
Abstract: In the original version of this Article, the author Anna Rita Fetoni was incorrectly indexed. This error has now been corrected.
Published: 2020

49. Effects of defective herpes simplex vectors expressing neurotrophic factors on the proliferation and differentiation of nervous cells in vivo

Author: Marconi, P, Zucchini, S, Berto, E, Bozac, A, Paradiso, B, Bregola, G, Grassi, C, Volpi, I, Argnani, R, Marzola, A, Manservigi, R, and Simonato, M
Published: 2005
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50. Manejo nutricional de ascitis quilosa: Serie de casos y revisión de la literatura

Author: Valenzuela P, María José, primary, Jofré M, Paulina, additional, Reimer C, Camila, additional, Valdés B, Silvana, additional, and Grassi C, Bruno, additional
Published: 2020
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