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3. Evaluation of three hemagglutinin-based vaccines for the experimental control of a panzootic clade 2.3.4.4b A(H5N8) high pathogenicity avian influenza virus in mule ducks

Author

Niqueux, Éric, Flodrops, Marion, Allée, Chantal, Lebras, Marie-Odile, Pierre, Isabelle, Louboutin, Katell, Guillemoto, Carole, Le Prioux, Aurélie, Le Bouquin-Leneveu, Sophie, Keïta, Alassane, Amelot, Michel, Martenot, Claire, Massin, Pascale, Cherbonnel-Pansart, Martine, Briand, François-Xavier, Schmitz, Audrey, Cazaban, Christophe, Dauphin, Gwenaëlle, Delquigny, Thomas, Lemière, Stéphane, Watier, Jean-Marie, Mogler, Mark, Tarpey, Ian, Grasland, Béatrice, and Eterradossi, Nicolas

Published
2022
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6. Highly Pathogenic Avian Influenza A(H5N1) Clade 2.3.4.4b Virus in Domestic Cat, France, 2022

Author

Briand, François-Xavier, primary, Souchaud, Florent, additional, Pierre, Isabelle, additional, Beven, Véronique, additional, Hirchaud, Edouard, additional, Hérault, Fabrice, additional, Planel, René, additional, Rigaudeau, Angélina, additional, Bernard-Stoecklin, Sibylle, additional, Van der Werf, Sylvie, additional, Lina, Bruno, additional, Gerbier, Guillaume, additional, Eterradossi, Nicolas, additional, Schmitz, Audrey, additional, Niqueux, Eric, additional, and Grasland, Béatrice, additional

Published
2023
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9. Evaluation of three hemagglutinin-based vaccines for the experimental control of a panzootic clade 2.3.4.4b A(H5N8) high pathogenicity avian influenza virus in mule ducks

Author

Niqueux, Éric, primary, Flodrops, Marion, additional, Allée, Chantal, additional, Lebras, Marie-Odile, additional, Pierre, Isabelle, additional, Louboutin, Katell, additional, Guillemoto, Carole, additional, Le Prioux, Aurélie, additional, Le Bouquin-Leneveu, Sophie, additional, Keïta, Alassane, additional, Amelot, Michel, additional, Martenot, Claire, additional, Massin, Pascale, additional, Cherbonnel-Pansart, Martine, additional, Briand, François-Xavier, additional, Schmitz, Audrey, additional, Cazaban, Christophe, additional, Dauphin, Gwenaëlle, additional, Delquigny, Thomas, additional, Lemière, Stéphane, additional, Watier, Jean-Marie, additional, Mogler, Mark, additional, Tarpey, Ian, additional, Grasland, Béatrice, additional, and Eterradossi, Nicolas, additional

Published
2023
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10. Can machine learning models help us with pathotyping infectious bursal disease virus strains?

Author

Molinet, Annonciade, Courtillon, Céline, Amelot, Michel, Keita, Alassane, Grasland, Béatrice, Eterradossi, Nicolas, Soubies, Sébastien, Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and Université de Rennes (UR)

Subjects
[SDV]Life Sciences [q-bio], Machine learning, Infectious bursal disease virus
Abstract

National audience

Published
2022

11. Multiple independent introductions of highly pathogenic avian influenza H5 viruses during the 2020–2021 epizootic in France

Author

Briand, François‐Xavier, primary, Niqueux, Eric, additional, Schmitz, Audrey, additional, Martenot, Claire, additional, Cherbonnel, Martine, additional, Massin, Pascale, additional, Busson, Rachel, additional, Guillemoto, Carole, additional, Pierre, Isabelle, additional, Louboutin, Katell, additional, Souchaud, Florent, additional, Allée, Chantal, additional, Quenault, Helene, additional, Lucas, Pierrick, additional, de Wiele, Anne Van, additional, Blanchard, Yannick, additional, Eterradossi, Nicolas, additional, Scoizec, Axelle, additional, Bouquin‐Leneveu, Sophie Le, additional, Rautureau, Severine, additional, Lambert, Yves, additional, and Grasland, Béatrice, additional

Published
2022
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12. Concomitant NA and NS deletion on avian Influenza H3N1 virus associated with hen mortality in France in 2019

Author

Briand, François-Xavier, primary, Schmitz, Audrey, additional, Scoizec, Axelle, additional, Allée, Chantal, additional, Busson, Rachel, additional, Guillemoto, Carole, additional, Quenault, Hélène, additional, Lucas, Pierrick, additional, Pierre, Isabelle, additional, Louboutin, Katell, additional, Guillou-Cloarec, Cécile, additional, Martenot, Claire, additional, Cherbonnel-Pansart, Martine, additional, Thomas, Rodolphe, additional, Massin, Pascale, additional, Souchaud, Florent, additional, Blanchard, Yannick, additional, Steensels, Mieke, additional, Lambrecht, Benedicte, additional, Eterradossi, Nicolas, additional, Le Bouquin, Sophie, additional, Niqueux, Eric, additional, and Grasland, Béatrice, additional

Published
2022
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13. Full-Length Genome Sequence of a Novel European Antigenic Variant Strain of Infectious Bursal Disease Virus

Author

Molinet, Annonciade, primary, Courtillon, Céline, additional, Le Men, Maryvonne, additional, Amenna-Bernard, Nadia, additional, Retaux, Charlotte, additional, Leroux, Aurélie, additional, Lucas, Pierrick, additional, Blanchard, Yannick, additional, Nooruzzaman, Mohammed, additional, Islam, Mohammad Rafiqul, additional, Briand, François-Xavier, additional, Grasland, Béatrice, additional, Eterradossi, Nicolas, additional, and Soubies, Sébastien, additional

Published
2022
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14. Infectious Bronchitis Coronavirus: Genome Evolution in Vaccinated and Non-Vaccinated SPF Chickens

Author

Flageul, Alexandre, primary, Allée, Chantal, additional, Courtillon, Céline, additional, Béven, Véronique, additional, Quenault, Hélène, additional, Blanchard, Yannick, additional, Amelot, Michel, additional, Courtois, David, additional, De Wit, Sjaak, additional, Eterradossi, Nicolas, additional, Grasland, Béatrice, additional, and Brown, Paul A., additional

Published
2022
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16. Impact du pathotype du virus de la bursite infectieuse aviaire sur l’activité transcriptionnelle in vivo des cellules de la bourse de Fabricius infectées

Author

Molinet, Annonciade, Courtillon, Céline, Lucas, Pierrick, Hirchaud, Edouard, Amelot, Michel, Keïta, Alassane, Blanchard, Yannick, Grasland, Béatrice, Eterradossi, Nicolas, Soubies, Sébastien M., Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université de Rennes - UFR Sciences de la vie et de l'environnement (UR SVE), Université de Rennes (UR), and Duborgel, Françoise

Subjects
Bursite infectieuse aviaire, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, [SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Abstract

International audience; Pas de résumé disponible

Published
2022

17. Maladie de Gumboro : impact du pathotype viral sur le profil leucocytaire sanguin

Author

Molinet, Annonciade, Courtillon, Céline, Amelot, Michel, Keita, Alassane, Grasland, Béatrice, Eterradossi, Nicolas, Soubies, Sébastien, Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and Université de Rennes (UR)

Subjects
Bursite infectieuse aviaire, Maladie de gumboro, [SDV]Life Sciences [q-bio]
Abstract

National audience; Infectious bursal disease virus (IBDV), the etiological agent of Gumboro Disease, is a worldwide spread immunosuppressive virus. It infects young chicks (Gallus gallus) and targets B cells produced in the bursa of Fabricius. That potentially deadly infection induces immunosuppression in the survivors. IBDV are classified into 4 pathotypes depending on the mortality and immunosuppression they induce: vaccinal (non pathogenic), strictly immunosuppressive, classical virulent and very virulent. No pathotype specific predictive marker has yet been identified and the current way to assess this viral characteristic relies on animal experiment. This study purpose is to highlight pathotype specific, easily measurable differences in viral behavior and host response to the infection. For this purpose, specific-pathogen-free (SPF) chicken were inoculated with the same infectious dose of IBDV of very virulent, classical, strictly immunosuppressive or vaccinal strains. A daily clinical monitoring was performed. At 2 and 4 days post infection (dpi), the bursal viral load was measured using infectious titration and total blood cell count (quantifying B cells, T cells, monocytes, thrombocytes anderythrocytes) was carried-out. The clinical outcome of animals was consistent with the pathotype of the used strains. The viral load was i) higher at 2 than 4 dpi, ii) was partially correlated with the pathogenicity. The total blood cell count revealed : i) a near disappearance of B cells for pathogenic strains, ii) a decrease of T cells concentration at 2 dpi proportional to pathogenicity, iii) at 4 dpi, an increase of T cells concentration above the mock groups value for the strictly immunosuppressive strains, iv) a severe thrombocytopenia for very virulent and classical virulent strains. To conclude, different white blood cells concentration modifications induced by different pathotypes have been observed and provide new insights into the differences in physiopathology related to the viral pathotype.; Le virus de la bursite infectieuse aviaire (IBDV), agent causal de la maladie de Gumboro, est un virus immunodépresseur répandu mondialement. Il infecte les jeunes poulets (Gallus gallus) et présente un tropisme pour les lymphocytes B (LB) produits dans la bourse de Fabricius (BdF). L’infection, potentiellement mortelle, induit une immunodépression chez les survivants. Les IBDV sont classés, selon la mortalité et l’immunodépression induites, en 4 pathotypes : vaccinal (non pathogène), immunodépresseur strict, virulent classique, hypervirulent. Aucun marqueur viral prédictif du pathotype n’a encore été identifié, et son évaluation requiert des protocoles d’expérimentations in vivo souvent lourds. L’objectif de cette étude était de mettre en évidence des différences liées aux pathotypes viraux dans des paramètres physiologiques de l’hôte facilement mesurables. Pour cela, des poulets exempts d’organismes pathogènes spécifiés ont été inoculés avec une même dose infectieuse de souches d’IBDV hypervirulentes, virulente classique, immunodépressives strictes ou vaccinales. Un suivi clinique quotidien des animaux a ensuite été réalisé. A 2 et 4 jours post-infection (jpi) la charge virale infectieuse dans la Bdf a été mesurée par titrage infectieux et une numération de formule sanguine (NFS) (quantifiant LB, lymphocytes T (LT), monocytes, thrombocytes et érythrocytes) a été réalisée. L’évolution clinique des animaux a été cohérente avec le pathotype des virus employés. La charge infectieuse dans la bourse i) était plus importante à 2 jpi qu’à 4 jpi, ii) n’était que partiellement corrélée à la pathogénicité. Les NFS ont, quant à elles, révélé : i) une quasi-disparition des LB à 2 et 4 jpi pour tous les virus, ii) une diminution à 2 jpi de la concentration en LT d’autant plus marquée que la pathogénicité est importante, iii) à 4 jpi, une remontée de cette valeur, devenue supérieure à celle des témoins pour les virus immunodépresseurs stricts, iv) une thrombocytopénie importante à 2 et 4 jpi pour les virus hypervirulents et le virus classique. En conclusion, l’évolution de la concentration sanguine de certaines populations cellulaires observées ici dégage de nouvelles pistes de réflexion quant aux différences dans la physiopathologie liée au pathotype viral.

Published
2022

18. Infectious Bronchitis Coronavirus: Genome Evolution in Vaccinated and Non-Vaccinated SPF Chickens

Author

FAH veterinaire epidemiologie, Flageul, Alexandre, Allée, Chantal, Courtillon, Céline, Béven, Véronique, Quenault, Hélène, Blanchard, Yannick, Amelot, Michel, Courtois, David, De Wit, Sjaak, Eterradossi, Nicolas, Grasland, Béatrice, Brown, Paul A, FAH veterinaire epidemiologie, Flageul, Alexandre, Allée, Chantal, Courtillon, Céline, Béven, Véronique, Quenault, Hélène, Blanchard, Yannick, Amelot, Michel, Courtois, David, De Wit, Sjaak, Eterradossi, Nicolas, Grasland, Béatrice, and Brown, Paul A

Published
2022

22. Avian influenza outbreaks: evaluating the efficacy of cleaning and disinfection of vehicles and transport crates

Author

Huneau-Salaün, Adeline, primary, Scoizec, Axelle, additional, Thomas, Rodolphe, additional, Martenot, Claire, additional, Schmitz, Audrey, additional, Pierre, Isabelle, additional, Allée, Chantal, additional, Busson, Rachel, additional, Massin, Pascale, additional, Briand, François-Xavier, additional, Guillemoto, Carole, additional, Louboutin, Katell, additional, Souchaud, Florent, additional, Cherbonnel-Pansart, Martine, additional, Niqueux, Eric, additional, Grasland, Béatrice, additional, Souillard, Rozenn, additional, and Bouquin, Sophie Le, additional

Published
2022
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23. Genome Evolution of Two Genetically Homogeneous Infectious Bursal Disease Virus Strains During Passages in vitro and ex vivo in the Presence of a Mutagenic Nucleoside Analog

Author

Cubas-Gaona, Liliana L., Flageul, Alexandre, Courtillon, Céline, Briand, Francois-Xavier, Contrant, Maud, Bougeard, Stephanie, Lucas, Pierrick, Quenault, Hélène, Leroux, Aurélie, Keita, Alassane, Amelot, Michel, Grasland, Béatrice, Blanchard, Yannick, Eterradossi, Nicolas, Brown, Paul Alun, Soubies, Sébastien Mathieu, Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), LLC-G received a post-doctoral fellowship from 'Région Bretagne' (program 'Stratégie D’Attractivité Durable,' Grant Number 17002) and 'Agglomération de Saint-Brieuc'., and European Project: 731014,VetBioNet(2017)

Subjects
IBDV, animal structures, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, viruses, MESH: Infectious bursal disease virus, 7DMA, MESH: Chickens, Microbiology, QR1-502, mutation frequency, viral diversity, chicken B cells, evolution, MESH: 7-deaza-2'-C-methyladenosine, MESH: Genome, Viral, passages
Abstract

Theavibirnavirusinfectious bursal disease virus (IBDV) is responsible for a highly contagious and sometimes lethal disease of chickens (Gallus gallus). IBDV genetic variation is well-described for both field and live-attenuated vaccine strains, however, the dynamics and selection pressures behind this genetic evolution remain poorly documented. Here, genetically homogeneous virus stocks were generated using reverse genetics for a very virulent strain, rvv, and a vaccine-related strain, rCu-1. These viruses were serially passaged at controlled multiplicities of infection in several biological systems, including primary chickens B cells, the main cell type targeted by IBDVin vivo. Passages were also performed in the absence or presence of a strong selective pressure using the antiviral nucleoside analog 7-deaza-2′-C-methyladenosine (7DMA). Next Generation Sequencing (NGS) of viral genomes after the last passage in each biological system revealed that (i) a higher viral diversity was generated in segment A than in segment B, regardless 7DMA treatment and viral strain, (ii) diversity in segment B was increased by 7DMA treatment in both viruses, (iii) passaging of IBDV in primary chicken B cells, regardless of 7DMA treatment, did not select cell-culture adapted variants of rvv, preserving its capsid protein (VP2) properties, (iv) mutations in coding and non-coding regions of rCu-1 segment A could potentially associate to higher viral fitness, and (v) a specific selection, upon 7DMA addition, of a Thr329Ala substitution occurred in the viral polymerase VP1. The latter change, together with Ala270Thr change in VP2, proved to be associated with viral attenuationin vivo. These results identify genome sequences that are important for IBDV evolution in response to selection pressures. Such information will help tailor better strategies for controlling IBDV infection in chickens. &nbsp

Published
2021

24. Description of the first isolates of Guinea fowl corona and picornaviruses obtained from a case of Guinea fowl fulminating enteritis.

Author

Courtillon, Céline, primary, Briand, François-Xavier, additional, Allée, Chantal, additional, Contrant, Maud, additional, Beven, Véronique, additional, Lucas, Pierrick, additional, Blanchard, Yannick, additional, Mouchel, Simon, additional, Eterradossi, Nicolas, additional, Delforterie, Yves, additional, Grasland, Béatrice, additional, and Brown, Paul, additional

Published
2021
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25. Highly Pathogenic Avian Influenza A(H5N8) Virus Spread by Short- and Long-Range Transmission, France, 2016–17

Author

Briand, François-Xavier, primary, Niqueux, Eric, additional, Schmitz, Audrey, additional, Martenot, Claire, additional, Cherbonnel, Martine, additional, Massin, Pascale, additional, Kerbrat, Florian, additional, Chatel, Marina, additional, Guillemoto, Carole, additional, Guillou-Cloarec, Cecile, additional, Ogor, Katell, additional, Le Prioux, Aurélie, additional, Allée, Chantal, additional, Beven, Véronique, additional, Hirchaud, Edouard, additional, Blanchard, Yannick, additional, Scoizec, Axelle, additional, Le Bouquin, Sophie, additional, Eterradossi, Nicolas, additional, and Grasland, Béatrice, additional

Published
2021
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27. Natural and Experimental Persistence of Highly Pathogenic H5 Influenza Viruses in Slurry of Domestic Ducks, with or without Lime Treatment

Author

Schmitz, Audrey, primary, Pertusa, Marion, additional, Le Bouquin, Sophie, additional, Rousset, Nathalie, additional, Ogor, Katell, additional, LeBras, Marie-Odile, additional, Martenot, Claire, additional, Daniel, Patrick, additional, Belen Cepeda Hontecillas, Ana, additional, Scoizec, Axelle, additional, Morin, Hervé, additional, Massin, Pascale, additional, Grasland, Béatrice, additional, Niqueux, Eric, additional, and Eterradossi, Nicolas, additional

Published
2020
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28. PCV2 co-infection does not impact PRRSV MLV1 safety but enhances virulence of a PRRSV MLV1-like strain in infected SPF pigs

Author

Eclercy, Julie, primary, Larcher, Thibaut, additional, Andraud, Mathieu, additional, Renson, Patricia, additional, Bernard, Cécilia, additional, Bigault, Lionel, additional, Ledevin, Mireille, additional, Paboeuf, Frédéric, additional, Grasland, Béatrice, additional, Rose, Nicolas, additional, and Bourry, Olivier, additional

Published
2020
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31. Comparison of pathogenicity and intestinal immunity outcomes in infected pigs with French InDel and US non-InDel strains of porcine epidemic diarrhea virus

Author

Gallien, Sarah, Rossignol, Christelle, Fleurot, Isabelle, Chevaleyre, Claire, Bernard, C., Bigault, Lionel, Melo, Sandrina, Moro, Angélique, Lediguerher, Gérald, Paboeuf, Frédéric, Rose, Nicolas, Grasland, Béatrice, Berri, Mustapha, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Bretagne Loire (COMUE) (UBL), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, [SDV.IMM]Life Sciences [q-bio]/Immunology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

32. Comparaison de la transmission horizontale d’une souche française InDel et d’une souche US non-InDel de virus de la diarrhée épidémique porcine (PEDV)

Author

Gallien, Sarah, Andraud, Mathieu, Moro, Angélique, Morin, Nadège, Lediguerher, Gérald, Paboeuf, Frédéric, Berri, Mustapha, Rose, Nicolas, Grasland, Béatrice, Laboratoire de Ploufragan - Plouzané, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Société Française de Virologie (SFV). FRA., and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

34. Natural viral co‐infections in pig herds affect hepatitis E virus (HEV) infection dynamics and increase the risk of contaminated livers at slaughter

Author

Salines, Morgane, primary, Dumarest, Marine, additional, Andraud, Mathieu, additional, Mahé, Sophie, additional, Barnaud, Elodie, additional, Cineux, Maelan, additional, Eveno, Eric, additional, Eono, Florent, additional, Dorenlor, Virginie, additional, Grasland, Béatrice, additional, Bourry, Olivier, additional, Pavio, Nicole, additional, and Rose, Nicolas, additional

Published
2019
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35. Limited shedding of an S-InDel strain of porcine epidemic diarrhea virus (PEDV) in semen and questions regarding the infectivity of the detected virus

Author

Gallien, Sarah, primary, Moro, Angélique, additional, Lediguerher, Gérald, additional, Catinot, Virginie, additional, Paboeuf, Frédéric, additional, Bigault, Lionel, additional, Gauger, Phillip C., additional, Pozzi, Nathalie, additional, Berri, Mustapha, additional, Authié, Edith, additional, Rose, Nicolas, additional, and Grasland, Béatrice, additional

Published
2019
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36. Modification of PCV-2 virulence by substitution of the genogroup motif of the capsid protein

Author

Allemandou Aude, Grasland Béatrice, Hernandez-Nignol Anne-Cécile, Kéranflec'h André, Cariolet Roland, and Jestin André

Subjects
Veterinary medicine, SF600-1100
Abstract

Abstract Porcine circovirus type 2 (PCV-2) is the causal agent of the post-weaning multisystemic wasting syndrome (PMWS). PCV-2 are small single-stranded circular DNA viruses clustered into two main genogroups: PCV-2a and PCV-2b. Each genogroup present a specific highly-conserved motif of six amino acids (between amino acids 86 and 91) in the PCV-2 capsid protein. The aim of this study was to verify whether the motif located in the capsid protein and specific to each PCV-2 genogroup contributes to virulence. Two parental DNA clones, PCV-2a and PCV-2b, were constructed as well as two mutants DNA clones, PCV-2a/motif 2b and PCV-2b/motif 2a by exchanging the capsid motif of each genogroup. The four DNA clones were characterized in vitro as well as in vivo. Cells transfected by the four DNA clones produced infectious viruses. In specific-pathogen-free piglets transfected by the four infectious DNA clones, PCV-2b/motif 2a virulence was not attenuated while the PCV-2a/motif 2b virulence was drastically reduced compared to their parent virulence. These results suggest that the amino acids between positions 86 and 91 of the capsid protein are determinant for the virulence of isolates. However, the environment of this motif seems also involved.

Published
2011
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38. Replication of porcine circoviruses

Author

Grasland Béatrice, Dory Daniel, Faurez Florence, and Jestin André

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract Porcine circoviruses are circular single-stranded DNA viruses that infect swine and wild boars. Two species of porcine circoviruses exist. Porcine circovirus type 1 is non pathogenic contrary to porcine circovirus type 2 which is associated with the disease known as Post-weaning Multisystemic Wasting Syndrome. Porcine circovirus DNA has been shown to replicate by a rolling circle mechanism. Other studies have revealed similar mechanisms of rolling-circle replication in plasmids and single-stranded viruses such as Geminivirus. Three elements are important in rolling-circle replication: i) a gene encoding initiator protein, ii) a double strand origin, and iii) a single strand origin. However, differences exist between viruses and plasmids and between viruses. Porcine circovirus replication probably involves a "melting pot" rather than "cruciform" rolling-circle mechanism. This review provides a summary of current knowledge of replication in porcine circoviruses as models of the Circovirus genus. Based on various studies, the factors affecting replication are defined and the mechanisms involved in the different phases of replication are described or proposed.

Published
2009
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39. Full-length genome sequences of porcine epidemic diarrhoea virus strain CV777; use of NGS to analyse genomic and sub-genomic RNAs

Author

Rasmussen, Thomas Bruun, Boniotti, Maria Beatrice, Papetti, Alice, Grasland, Béatrice, Frossard, Jean Pierre, Dastjerdi, Akbar, Hulst, Marcel, Hanke, Dennis, Pohlmann, Anne, Blome, Sandra, Van Der Poel, Wim H.M., Steinbach, Falko, Blanchard, Yannick, Lavazza, Antonio, Bøtner, Anette, Belsham, Graham J., Rasmussen, Thomas Bruun, Boniotti, Maria Beatrice, Papetti, Alice, Grasland, Béatrice, Frossard, Jean Pierre, Dastjerdi, Akbar, Hulst, Marcel, Hanke, Dennis, Pohlmann, Anne, Blome, Sandra, Van Der Poel, Wim H.M., Steinbach, Falko, Blanchard, Yannick, Lavazza, Antonio, Bøtner, Anette, and Belsham, Graham J.

Abstract

Porcine epidemic diarrhoea virus, strain CV777, was initially characterized in 1978 as the causative agent of a disease first identified in the UK in 1971. This coronavirus has been widely distributed among laboratories and has been passaged both within pigs and in cell culture. To determine the variability between different stocks of the PEDV strain CV777, sequencing of the full-length genome (ca. 28kb) has been performed in 6 different laboratories, using different protocols. Not surprisingly, each of the different full genome sequences were distinct from each other and from the reference sequence (Accession number AF353511) but they are >99% identical. Unique and shared differences between sequences were identified. The coding region for the surface-exposed spike protein showed the highest proportion of variability including both point mutations and small deletions. The predicted expression of the ORF3 gene product was more dramatically affected in three different variants of this virus through either loss of the initiation codon or gain of a premature termination codon. The genome of one isolate had a substantially rearranged 5´-terminal sequence. This rearrangement was validated through the analysis of sub-genomic mRNAs from infected cells. It is clearly important to know the features of the specific sample of CV777 being used for experimental studies.

Published
2018

40. Sequence diversity of CV777 PEDV strains

Author

Rasmussen, Thomas Bruun, Boniotti, Maria Beatrice, Papetti, Alice, Grasland, Béatrice, Frossard, Jean Pierre, Dastjerdi, Akbar, Hulst, M.M., Hanke, Dennis, Pohlmann, Anne, Blome, Sandra, van der Poel, W.H.M., Steinbach, Falko, Blanchard, Yannick, Lavazza, Antonio, Bøtner, Anette, Belsham, Graham J., Rasmussen, Thomas Bruun, Boniotti, Maria Beatrice, Papetti, Alice, Grasland, Béatrice, Frossard, Jean Pierre, Dastjerdi, Akbar, Hulst, M.M., Hanke, Dennis, Pohlmann, Anne, Blome, Sandra, van der Poel, W.H.M., Steinbach, Falko, Blanchard, Yannick, Lavazza, Antonio, Bøtner, Anette, and Belsham, Graham J.

Published
2018

41. Comparison of the horizontal transmission of a French InDel strain and a US non InDel strain of porcine epidemic diarrhea virus

Author

Gallien, Sarah, Andraud, Mathieu, Bigault, Lionel, Moro, Angélique, Morin, Nathalie, Lediguerher, Gérald, Berri, Mustapha, Paboeuf, Frédéric, Rose, N., Grasland, Béatrice, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Laboratoire de Ploufragan, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Bretagne Loire (COMUE) (UBL), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES). FRA., and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2017

42. Assessment of within and between-pen transmission of porcine epidemic diarrhea virus InDel strain

Author

Gallien, Sarah, Andraud, Mathieu, Bigault, Lionel, Berri, Mustapha, Paboeuf, Frédéric, Rose, N., Grasland, Béatrice, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Laboratoire de Ploufragan, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Bretagne Loire (COMUE) (UBL), Kansas State University. USA., and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

43. Better horizontal transmission of a US non-InDel strain compared with a French InDel strain of porcine epidemic diarrhoea virus

Author

Gallien, Sarah, primary, Andraud, Mathieu, additional, Moro, Angélique, additional, Lediguerher, Gérald, additional, Morin, Nadège, additional, Gauger, Phillip C., additional, Bigault, Lionel, additional, Paboeuf, Frédéric, additional, Berri, Mustapha, additional, Rose, Nicolas, additional, and Grasland, Béatrice, additional

Published
2018
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44. Full-length genome sequences of porcine epidemic diarrhoea virus strain CV777; Use of NGS to analyse genomic and sub-genomic RNAs

Author

Rasmussen, Thomas Bruun, primary, Boniotti, Maria Beatrice, additional, Papetti, Alice, additional, Grasland, Béatrice, additional, Frossard, Jean-Pierre, additional, Dastjerdi, Akbar, additional, Hulst, Marcel, additional, Hanke, Dennis, additional, Pohlmann, Anne, additional, Blome, Sandra, additional, van der Poel, Wim H. M., additional, Steinbach, Falko, additional, Blanchard, Yannick, additional, Lavazza, Antonio, additional, Bøtner, Anette, additional, and Belsham, Graham J., additional

Published
2018
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45. Evidence of porcine epidemic diarrhea virus (PEDV) shedding in semen from infected specific pathogen-free boars

Author

Gallien, Sarah, primary, Moro, Angélique, additional, Lediguerher, Gérald, additional, Catinot, Virginie, additional, Paboeuf, Frédéric, additional, Bigault, Lionel, additional, Berri, Mustapha, additional, Gauger, Phillip C., additional, Pozzi, Nathalie, additional, Authié, Edith, additional, Rose, Nicolas, additional, and Grasland, Béatrice, additional

Published
2018
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46. Full-length genome sequences of porcine epidemic diarrhoea virus strain CV777; Use of NGS to analyse genomic and sub-genomic RNAs

Author

Melcher, Ulrich, Rasmussen, Thomas Bruun, Boniotti, Maria Beatrice, Papetti, Alice, Grasland, Béatrice, Frossard, Jean-Pierre, Dastjerdi, Akbar, Hulst, Marcel, Hanke, Dennis, Pohlmann, Anne, Blome, Sandra, van der Poel, Wim H. M., Steinbach, Falko, Blanchard, Yannick, Lavazza, Antonio, Bøtner, Anette, and Belsham, Graham J.

Subjects
0301 basic medicine, Swine, Cell Lines, lcsh:Medicine, medicine.disease_cause, Biochemistry, Genome, Database and Informatics Methods, Start codon, Coding region, Genomic library, DNA libraries, lcsh:Science, Phylogeny, Sequence Deletion, Coronavirus, Swine Diseases, Mammals, Genetics, Viral Genomics, Multidisciplinary, High-Throughput Nucleotide Sequencing, Eukaryota, Genomics, General Medicine, Virology & Molecular Biology, Nucleic acids, Vertebrates, RNA, Viral, Biological Cultures, Coronavirus Infections, General Agricultural and Biological Sciences, Sequence Analysis, Research Article, Animal Breeding & Genomics, Bioinformatics, Sequence analysis, 030106 microbiology, Genome, Viral, Microbial Genomics, Biology, Research and Analysis Methods, Microbiology, General Biochemistry, Genetics and Molecular Biology, Evolution, Molecular, Open Reading Frames, 03 medical and health sciences, SDG 3 - Good Health and Well-being, Sequence Motif Analysis, Virology, medicine, Life Science, Animals, Point Mutation, Fokkerij & Genomica, Vero Cells, Sequence Assembly Tools, Base Sequence, Sequence Analysis, RNA, Porcine epidemic diarrhea virus, lcsh:R, Organisms, Biology and Life Sciences, Computational Biology, DNA, Genome Analysis, Genomic Libraries, biology.organism_classification, Virologie & Moleculaire Biologie, 030104 developmental biology, Amniotes, lcsh:Q, Sequence Alignment, Reference genome
Abstract

Porcine epidemic diarrhoea virus, strain CV777, was initially characterized in 1978 as the causative agent of a disease first identified in the UK in 1971. This coronavirus has been widely distributed among laboratories and has been passaged both within pigs and in cell culture. To determine the variability between different stocks of the PEDV strain CV777, sequencing of the full-length genome (ca. 28kb) has been performed in 6 different laboratories, using different protocols. Not surprisingly, each of the different full genome sequences were distinct from each other and from the reference sequence (Accession number AF353511) but they are >99% identical. Unique and shared differences between sequences were identified. The coding region for the surface-exposed spike protein showed the highest proportion of variability including both point mutations and small deletions. The predicted expression of the ORF3 gene product was more dramatically affected in three different variants of this virus through either loss of the initiation codon or gain of a premature termination codon. The genome of one isolate had a substantially rearranged 5´-terminal sequence. This rearrangement was validated through the analysis of sub-genomic mRNAs from infected cells. It is clearly important to know the features of the specific sample of CV777 being used for experimental studies.

Published
2018

49. Diarrhée épidémique porcine : le retour d'une « ancienne » maladie.

Author

Gallien, Sarah, Le Poder, Sophie, Rose, Nicolas, and Grasland, Béatrice

Abstract

Copyright of Virologie is the property of John Libbey Eurotext Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2016
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