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Flavonoids, including proanthocyanidins (PAs), anthocyanins and flavonols are essential secondary metabolites that contribute to the nutritional value and sensory quality of grape berry and red wine. Advances in molecular biology technology have led to substantial progress in understanding the regulation of flavonoid biosynthesis. The influence of terroir on grape berries and wine has garnered increasing attention, yet its comprehensive regulatory network remains underexplored. In terms of application, environmental factors such as water, light, and temperature are more easily regulated in grapevines compared to soil conditions. Therefore, we summarize their effects on flavonoid content and composition, constructing a network that links environmental factors, hormones, and metabolites to provide a deeper understanding of the underlying mechanisms. This review enriches the knowledge of the regulatory network mechanisms governing flavonoid responses to environmental factors in grapes. [ABSTRACT FROM AUTHOR]

Introduction: Exogenous melatonin (MT) can promote horticultural crops growth under stress conditions. Methods: In this study, the effects of exogenous MT on the accumulation of selenium (Se) in grape were studied under Se stress. Results and discussion: Under Se stress, exogenous MT increased the biomass, content of photosynthetic pigments and antioxidant enzyme activity of grapevines. Compared with Se treatment, MT increased the root biomass, shoot biomass, chlorophyll a content, chlorophyll b content, carotenoids, superoxide dismutase activity, and peroxidase activity by 18.11%, 7.71%, 25.70%, 25.00%, 25.93%, 5.73%, and 9.41%, respectively. Additionally, MT increased the contents of gibberellin, auxin, and MT in grapevines under Se stress, while it decreased the content of abscisic acid. MT increased the contents of total Se, organic Se and inorganic Se in grapevines. Compared with Se treatment, MT increased the contents of total Se in the roots and shoots by 48.82% and 135.66%, respectively. A transcriptome sequencing analysis revealed that MT primarily regulated the cellular, metabolic, and bioregulatory processes of grapevine under Se stress, and the differentially expressed genes (DEGs) were primarily enriched in pathways, such as aminoacyl-tRNA biosynthesis, spliceosome, and flavonoid biosynthesis. These involved nine DEGs and nine metabolic pathways in total. Moreover, a field experiment showed that MT increased the content of Se in grapes and improved their quality. Therefore, MT can alleviate the stress of Se in grapevines and promote their growth and the accumulation of Se. [ABSTRACT FROM AUTHOR]

BACKGROUND: The influences of abscisic acid (ABA) applications on precursors and gene expression in 3‐alkyl‐2‐methoxypyrazines (MPs) biosynthetic pathway, MPs concentration and sensory evaluation of its derived peculiar odors in Cabernet Sauvignon grapes and wines were investigated. At the vineyard, ABA solution with 25, 100 and 400 mg L−1 (AT1, AT2 and AT3, respectively) and an aqueous solution (control) were sprayed three times from veraison to pre‐harvest. RESULTS: Higher concentration ABA applications (AT2 and AT3) in grapes could significantly reduce MPs concentration and its derived peculiar odors in grapes and wines compared to a lower concentration ABA application (AT1) and control, with AT2 application having the strongest effect. The changes in MPs were mainly a result of the downregulated expression of VvOMTs genes at higher concentration ABA applications, independent of the levels of their potential precursors. CONCLUSION: The present study reveals that ABA application had the potential to decrease production of MPs in Cabernet Sauvignon grapes and wines, and this result provides reference values for the removal of unpleasant vegetable odors from Cabernet Sauvignon wines in production. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Grape seed oil (GSO) is a valuable resource for the pharmaceutical, cosmetic, and food industries due to its fatty acid composition, which provides anti-inflammatory, cardioprotective, antimicrobial, and anticancer properties. GSO is also recognized for its sensory attributes and serves as an essential component in our dietary intake, providing vital fatty acids for metabolic reactions. The enzymatic synthesis of structured lipids (SL) using GSO was investigated in this study. The SL was created by incorporating medium chain fatty acids (capric acid – C10) at the sn -1 and sn -3 positions and long chain fatty acids in the internal position of the triacylglycerols (TAG). GSO was selected based on its composition rich in unsaturated fatty acids, mainly linoleic acid. The acidolysis reactions were catalyzed by an immobilized commercial lipase Lipozyme TLIM®. The incorporation degree of C10 into GSO reached 36%. Health lipid indices were calculated to determine the C10 incorporation effect on the improvement of nutritional quality of oil. The SL showed lower oxidative stability index (OSI) values than the original oil and higher degree of fatty acid (DFA) values. The simpler, solvent-free method not only enhances the health benefits of oils but also gives valuable properties to GSO, making it an even more attractive option for food industries. [Display omitted] • Sustainable green processes for valorization of grape seed are proposed. • Enzymatic synthesis of structured lipids enhances GSO with health promoting C10 fatty acids. • Lipozyme TLIM¬Æ achieved a remarkable 36% C10 incorporation in GSO. • Simpler, solvent-free method improves GSO's health lipid indices, making it an ideal choice for food industries. [ABSTRACT FROM AUTHOR]

In grape (Vitis vinifera L.) cultivation, high temperatures (HTs) usually reduce the accumulation of anthocyanins. In order to elucidate the regulatory mechanism of anthocyanin biosynthesis under high-temperature environments, we investigated the effects of HT stress at veraison (5% coloring of grape ears) on fruit coloration and anthocyanin biosynthesis in 'Summer Black' (XH) and 'Flame seedless' (FL) grapevines. Compared to the control group (35 °C), the total anthocyanin content of XH and FL grapes subjected to a high-temperature (HT) treatment group (40 °C) decreased significantly as the HT treatment continued, but showed an upward trend with fruit development. However, the concentration of procyanidins increased significantly following HT treatment but decreased with fruit development. Nonetheless, FL grapes showed some resistance to the HT condition, producing anthocyanin content at ripeness comparable to the control group, demonstrating a greater adaptability to HT conditions than XH grapes. Based on the CIRG index, at stage S4, the fruit of FL was classified as dark red, while XH was classified as blue-black in the control group. Anthocyanin-targeted metabonomics identified eight different types of anthocyanins accumulating in the peels of XH and FL grapes during ripening, including cyanidins, delphinidins, malvidins, pelargonidins, peonidins, petunidins, procyanidins, and flavonoids. Malvidins were the most abundant in the two grape varieties, with malvidin-3-O-glucoside being more sensitive to high temperatures. HT treatment also down-regulated the expression of structural genes and regulators involved in the anthocyanin synthesis pathways. We used the WGCNA method to identify two modules that were significantly correlated with total anthocyanin and procyanidin contents. Among them, MYBCS1, bHLH137, WRKY65, WRKY75, MYB113-like, bZIP44, and GST3 were predicted to be involved in grape anthocyanin biosynthesis. In conclusion, this study conducted in-depth research on the HT inhibition of the biosynthesis of anthocyanins in XH and FL grapes, for reference. [ABSTRACT FROM AUTHOR]

Main conclusion: Supplying monochromatic blue LED light during the day, but not at night, promotes early coloration and improves anthocyanin accumulation in the skin of grape berries. Specific light spectra, such as blue light, are known to promote the biosynthesis and accumulation of anthocyanins in fruit skins. However, research is scarce on whether supplement of blue light during different periods of one day can differ in their effect. Here, we compared the consequences of supplying blue light during the day and night on the accumulation of anthocyanins in pigmented grapevine (Vitis vinifera) berries. Two treatments of supplemented monochromatic blue light were tested, with light emitting diodes (LED) disposed close to the fruit zone, irradiating between 8:00 and 18:00 (Dayblue) or between 20:00 and 6:00 (Nightblue). Under the Dayblue treatment, berry coloration was accelerated and total anthocyanins in berry skins increased faster than the control (CK) and also when compared to the Nightblue condition. In fact, total anthocyanin content was similar between CK and Nightblue. qRT-PCR analysis indicated that Dayblue slightly improved the relative expression of the anthocyanin-structural gene UFGT and its regulator MYBA1. Instead, the expression of the light-reception and -signaling related genes CRY, HY5, HYH, and COP1 rapidly increased under Dayblue. This study provides insights into the effect of supplementing monochromatic LED blue light during the different periods of one day, on anthocyanins accumulation in the berry skin. [ABSTRACT FROM AUTHOR]

Introduction: Grape is of high economic value. Colletotrichum viniferum, a pathogen causing grape ripe rot and leaf spot, threatens grape production and quality. Methods: This study investigates the interplay between C. viniferum by Cytological study and transcriptome sequencing. Results: Different grapevine germplasms, V. vinifera cv. Thompson Seedless (TS), V. labrusca accession Beaumont (B) and V. piasezkii Liuba-8 (LB-8) were classified as highly sensitive, moderate resistant and resistant to C. viniferum, respectively. Cytological study analysis reveals distinct differences between susceptible and resistant grapes post-inoculation, including faster pathogen development, longer germination tubes, normal appressoria of C. viniferum and absence of white secretions in the susceptible host grapevine. To understand the pathogenic mechanisms of C. viniferum, transcriptome sequencing was performed on the susceptible grapevine "TS" identifying 236 differentially expressed C. viniferum genes. These included 56 effectors, 36 carbohydrate genes, 5 P450 genes, and 10 genes involved in secondary metabolism. Fungal effectors are known as pivotal pathogenic factors that modulate plant immunity and affect disease development. Agrobacterium-mediated transient transformation in Nicotiana benthamiana screened 10 effectors (CvA13877, CvA01508, CvA05621, CvA00229, CvA07043, CvA05569, CvA12648, CvA02698, CvA14071 and CvA10999) that inhibited INF1 (infestans 1, P. infestans PAMP elicitor) induced cell death and 2 effectors (CvA02641 and CvA11478) that induced cell death. Additionally, transcriptome analysis of "TS" in response to C. viniferum identified differentially expressed grape genes related to plant hormone signaling (TGA, PR1, ETR, and ERF1/2), resveratrol biosynthesis genes (STS), phenylpropanoid biosynthesis genes (PAL and COMT), photosynthetic antenna proteins (Lhca and Lhcb), transcription factors (WRKY, NAC, MYB, ERF, GATA, bHLH and SBP), ROS (reactive oxygen species) clearance genes (CAT, GSH, POD and SOD), and disease-related genes (LRR, RPS2 and GST). [ABSTRACT FROM AUTHOR]

One of the most significant dried foods is raisin, used directly as ingredients in confectionary and in the form of raisin paste used in sauces, fillings, baked goods, microwaveable coatings, and other culinary products to color them naturally. The purpose of this study is to investigate the effects of ultrasound pretreatment (53 kHz, 300 W, 30 °C, 300 s, in a mixture of 5% K2CO3+ 1% OO solution) and drying air temperatures (40–60 °C) on the drying behavior, color values, physicochemical properties (moisture content, VCC, TPC, RSC, and antioxidant capacity (DDPH)) and rehydration capacity of Sultani Çekirdeksiz grapes. Both the drying kinetic (drying time, drying rate, and effective moisture diffusivity) and the quality characteristics of grapes were significantly improved by ultrasound pretreatment (p < 0.05). In addition, the vitamin C (0.75–3.86 mg/g) and total phenolic content (1.31–1.80 mg GA/kg) of raisins were highly protected with the high drying temperature and ultrasound pretreatment. The antioxidant capacity (1.15–5.50 mM Trolox) of samples increased with drying temperature whereas the antioxidant capacity decreased with ultrasound application. The rehydration time of all samples was found around 800 min. As a result of this study, it can be said that the ultrasound application may be used successfully for drying grapes and that a greater degree of the nutritional value of the grapes can be maintained. [ABSTRACT FROM AUTHOR]

[Objective] Grape is one of the five varieties of fruit trees widely cultivated in China. Long-term excessive application of chemical fertilizer will destroy the microbial ecology, thus affecting the yield and quality of grapes. Compound microbial agent of different strains may be a more active and effective measure to improve the quality of grapes. [Method] The compound microbial agent (Brevibacillus brevis FJAT-0809-GLX, Bacillus velezensis FJAT-55034 and B. brevis FJAT-10623) were used to irrigate the roots, and the effects of the compound microbial agent on grape growth, fruit quality, rhizosphere soil enzymatic activity and diversity of culturable Bacillus were analyzed. [Result] The compound microbial agent promoted the growth of grape leaves, increased the contents of chlorophyll a and carotenoid, and the activities of POD and PPO. The chlorophyll b content, SOD and PPO activities of young grape fruits in the treatment group was 100%, 1.77% and 66.67% higher than that in the control group, respectively. The application of compound microbial agent promoted early ripening, fruit color transformation and quality of grapes. The single fruit weight was 29.06% higher than that of control group, and the soluble solid and soluble protein increased by 7.31% and 94.74%, respectively, compared with the control group. The PPO enzyme activity of ripe grape fruit in the treatment group was significantly higher than that in control group. In addition, the compound microbial agent significantly improved the activities of amylase, catalase, cellulase, sucrase, and the number and species of Bacillus in grape rhizosphere soil. Correlation analysis showed that Bacillus zanthoxyli and Bacillus pseudomycoides promoted grape leaf growth and fruit quality. [Conclusion] The compound microbial agent may improve soil, promote grape growth and increase fruit quality. [ABSTRACT FROM AUTHOR]

Drought stress is one of the most important limitations in the production of agricultural products and especially horticulture in different parts of the world and Iran. The climate changes that have happened in the last few years, it is possible to provide sustainable production of grapes in water stress conditions by examining and selecting cultivars that tolerate to drought stress. For this purpose, an experiment from 2018 to 2022 in the form of split plots in the form of randomized complete blocks with 3 replications in garden conditions to investigate the effect of changes in soil water potential on the morphological, physiological and biochemical characteristics of grafted cuttings on cultivar traits Tolerant to sensitive 7 grape (Vitis vinifera L.) cultivars were carried out. The treatments included 7 selected grape varieties (‘Bidaneh Ghermez’, ‘Bidaneh Sefid’, ‘Rashe’, ‘Sahani’, ‘Kare Royeh’, ‘Moulai’, and ‘Chafte’) as the base and red quince cultivar as scion and three levels of drought stress including humidity. Agricultural (control), 75% (moderate stress) and 55% (severe stress) were based on agricultural moisture discharge. The results showed that with more severe water stress, the amount of proline, ABA, activity of catalase and peroxidase, the expression of CBF4, NCED3, and DHN1 genes increased, as well as the amount of total chlorophyll were decreased. In this experiment, the highest proline, abscisic acid, catalase, peroxidase, NCED3, CBF4 and DHN1 gene expression were observed in this experiment under severe stress treatment. In general, ‘Bidaneh Ghermez’ cultivar grafted on the rootstocks of ‘Chafte’, ‘Moulai’ and ‘Kare Royeh’ had a higher potential for tolerance to drought stress than other grafted combinations tested in most of the traits. [ABSTRACT FROM AUTHOR]

Key message: The VlLOG11 mediates the cytokinin signaling pathway to regulate grape fruit setting. Fruit set, as an accepted agronomic trait, is inextricably linked with fruit quality and yield. Previous studies have demonstrated that exogenous treatment with the synthetic cytokinin analog, forchlorfenuron (CPPU), significantly enhances fruit set. In this study, a significant reduction in endogenous cytokinins was found by measuring the content of cytokinins in young grape berries after CPPU treatment. LONELY GUYs (VlLOGs), a key cytokinin-activating enzyme working in the biosynthesis pathway of cytokinins, exhibited differential expression. Some differentially expressed VlLOGs genes were presented by RNA seq data and their functions and regulation patterns were further investigated. The results showed that VlLOG11 was differentially expressed in young grape berries after CPPU treatment. Overexpression of VlLOG11 in tomato increases the amount of fruit set, and upregulated the expression of genes associated with cytokinin signaling including SlHK4, SlHK5, SlHP3, SlHP4, SlPHP1, SlPHP2. VlMYB4 and VlCDF3 could regulate the expression of VlLOG11 by directly binding to its promoter in young grape berries during fruit set. These results strongly demonstrated that VlMYB4/VlCDF3-VlLOG11 regulatory module plays a key role in the process of fruit setting in grape. This provided a basis for the molecular mechanism of VlLOG11-mediated cytokinin biosynthesis in young grape fruit set. [ABSTRACT FROM AUTHOR]

The WUSCHEL-related homeobox (WOX) transcription factor family plays critical roles in plant growth, development, and stress adaptation, but the biological functions in response to various stress of the WOX gene family have not been extensively researched in grapevine (Vitis vinifera). In this study, 12 grapevine WOXs were identified from the grapevine genome. Quantitative PCR and microarray expression profiling found that the expression of WOXs had an obvious tissue-specific pattern. Conjoint analysis between various tissues and treated materials indicated VvWUS1 expression is associated with expression of genes from grapevine rupestris stem pitting-associated virus; and VvWOX3 with grapevine fanleaf virus. The gene expression patterns of the WOXs in grape were different under salt stress, with VvWOX8/9 , VvWUS1 , and VvWOX3 responding more strongly to salt stress than control by 18.20-, 9.50-, and 9.19-fold. This study further improves understanding of the evolution and function of the WOX gene family, and offers a theoretical framework and reference for breeding grapevine to better tolerate adversity and permit cultivation of seedlings free of viruses. WOX is a family of genes that help regulate how plants develop and also respond to stress. In this study, 12 WOX genes were identified in grapes and analysed by bioinformatic methods. Coanalysis of different expression patterns in grapes showed that two of these genes respond to specific viruses and effectively block virus replication and infestation. Another gene was the most sensitive to salt stress. This study provides preliminary evidence that these three genes are key to resistance to salt and to viruses in grape, providing vital candidate genes for grapevine breeding [ABSTRACT FROM AUTHOR]

(1) Background: Spring frost damage is a common phenomenon that occurs in Southern Chile that considerably affects vine productivity and grape quality. (2) Methods: A field trial was conducted in order to study vine phenology and berry physicochemical parameters in Chardonnay, Sauvignon Blanc and Pinot Noir ungrafted and grafted (onto 101-14 Mgt rootstock) grapevines after a spring frost. This event killed the totality of primary bud shoots when the vines reached the phenological stage of unfolded leaves. (3) Results: From budburst, to flowering of secondary bud shoots, ungrafted Sauvignon Blanc grapevines presented an advanced phenology, whereas 101-14 Mgt rootstock tended to advance the maturity of Pinot Noir grapevines from flowering to ripening of berries. At harvest, berries from secondary buds of vines grafted onto 101-14 Mgt rootstock showed higher soluble solids than the ones from ungrafted Chardonnay and Sauvignon Blanc vines. High total phenolic content was found in berries from secondary buds of the grafted vines, compared to the ones from the ungrafted vines. Berry soluble solids variability tended to statistically decrease toward harvest in the studied plant materials, and the maximum coefficient of variation for soluble solids, berry weight, berry firmness and berry size reached 9.5%, 25.9%, 18.6% and 8.9%, respectively. (4) Conclusions: These preliminary results may be interesting for the Southern Chilean viticulturists since it seems that 101-14 Mgt rootstock could affect phenology and grape berry maturity of grapevines established in the Cautín Valley after spring frost damage. [ABSTRACT FROM AUTHOR]

Charcoal-based fertilizer could be used extensively and is environmentally friendly. An experiment was designed to investigate the effects of different charcoal-based fertilizer application methods on soil microbiology and grape quality in a vineyard to guide the cultivation of 'Shine-Muscat'. A control treatment without fertilization and six other treatments were set up. Four treatments applied carbon-based fertilizer as a base fertilizer with or without potassium fulvic acid, a complex microbial agent, or Bacillus subtilis, and two treatments were only applied with two applications of carbon-based fertilizer or compound fertilizer during the expansion period. The results showed that the bacterial phyla were mainly Proteobacteria and Bacteroidetes. Ascomycota, Basidiomycota, and Mortierellomycota dominated the fungal community. At the genus level, the composition of fungi, compared to bacteria, varied significantly, while the dominant flora differed among fertilization practices. Application of charcoal-based fertilizer enriched beneficial microorganisms, while chemical fertilizers enriched pathogenic microorganisms. The addition of microbial fungicides and biostimulants for a period reduced the size of the microbial network, lowered positive correlations, and enhanced resistance to adverse conditions and diseases and there was no significant correlation between agronomic traits and microbial network topology. A combination of soil microbial and grape agronomic traits suggests that a charcoal-based fertilizer base, with microbial fungicides applied, is the optimal fertilization regimen for grape. [ABSTRACT FROM AUTHOR]

The light plays a key role in regulating anthocyanin biosynthesis in plants. However, the molecular basis of anthocyanin synthesis in grape skins irradiated at night with supplemental white light (W), red light (R), blue light (B), and blue light for 3 h, 6 h, 9 h, and 12 h (B3, B6, B9, and B12) is not known. In the present study, the total anthocyanin content in grape skins was significant under different light (DL) and blue-light time (DT) treatments, and the best result was obtained with B9. Analysis of RNA-seq data determined that the key genes in the anthocyanin synthesis pathway, PAL, CHS, DFR, GT, CFIP, VIT_14s0068g00920, VIT_06s0009g02860, and VIT_06s0004g08150, were significantly up-regulated under night-time supplemental light treatments, which led to the significant expression of the phenylpropanoid biosynthesis, phenylalanine metabolism, flavonoid biosynthesis, flavone and flavonol biosynthesis, and the anthocyanin biosynthesis pathway, which were significantly expressed, promoting the accumulation of anthocyanin. The B caused significant expression of PAL (VIT_08s0040g01710), CFIP (VIT_13s0067g03820, VIT_13s0067g02870) and DFR (VIT_18s0001g12800), which may be one of the reasons for the better effect of B compared to W anthocyanin enrichment in grape skins. The DT treatment resulted in significant expression of GT (VIT_11s0052g01600), Peroxidase, VIT_09s0018g01190, and VIT_11s0037g00570. In addition, many TFs such as bHLH, MYB, ERF, WRKY, C2H2, MYB-related, and NAC were found to be involved in the synthesis of anthocyanins under light regulation. These results provide new insights into plants' nocturnal supplemental-light regulation of anthocyanin accumulation. [ABSTRACT FROM AUTHOR]

To understand the effects of different returning methods on soil nutrients,enzyme activity,and fruit quality in vineyards after intercropping rape as green manure. Through field experiments,three treatments were set up:a single grape tree without green manure,intercropping rape with green manure mulching,and intercropping rape with green manure ploughing. The results showed that the organic matter,total N,alkali-hydrolyzed N,available P,available K,urease,and alkaline phosphatase activity in the 0- 20 cm soil layer were the highest under the ploughing,increased significantly by 50. 00%,19. 40%,16. 47%,25. 38%,13. 69%,82. 35% and 26. 87% compared to the control,respectively. In the 20- 40 cm soil layer,compared with control soil pH,total N,available K,urease,and sucrase activities significantly increased by 3. 70%,5. 07%,44. 68%,40. 43%,14. 71%,20. 85%,32. 00%,35. 50%,11. 85% and 29. 84%,respectively,while the difference between mulching and ploughing was not significant. The hundred leaf dry weight,ear weight,soluble sugar,sugar acid to ratio,and vitamin C content of grapes were significantly higher than those of the control under mulching and ploughing treatments,and the highest was observed under ploughing treatments. Intercropping rape green manure could increase the soil nutrient content,promote grape growth,and improve fruit quality,and the effect of green manure ploughing was better than that of green manure mulching. [ABSTRACT FROM AUTHOR]

Summary: Grape is widely consumed all over the world for its rich nutritious and health‐promoting effects. While grape berries are prone to quality deterioration after harvest. This study aimed to investigate the effect of eugenol treatment on the quality of grape berries based on the transcriptome. Harvested grape berries were treated with eugenol, and then collected for nutrient component determination and transcriptomics analyses. As a results, treatment with eugenol enhanced the berries firmness, increased the contents of soluble sugars, organic acids and phenolic compounds, improved the antioxidant capacity, and altered gene expression levels of related pathways. Transcriptome analysis revealed 699 differentially expressed genes by 100 mmol L−1 eugenol‐treated berries. Among these DEGs, the transcriptional levels involved in the cell wall, sugar and acid metabolism, phenylpropane metabolism, antioxidant enzymes, and WRKY, MYB family transcription factors were significantly regulated. This study will help to promote the application of eugenol for quality improvements in grape berries. [ABSTRACT FROM AUTHOR]

AbstractGrapes are renowned for their health-promoting properties due to polyphenols, particularly anthocyanins in red grapes and flavonols in white grapes. This study evaluated the total antioxidant capacities (TAC) of specific grape varieties (Sultaniye, Chardonnay, Shiraz, Carignan, Alicante Bouschet, and Merlot) using the CUPRAC (CUPric Reducing Antioxidant Capacity) and ABTS/HRP (2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)/horseradish peroxidase) methods. Additionally, total phenolic, flavonoid, and anthocyanin contents were determined via Folin Ciocalteu, AlCl3/NaNO2, and pH differential methods. Primary phenolic compounds were identified with High-Performance Liquid Chromatography-Photodiode Array (HPLC-PDA). TAC values of the grape juices (mmol Trolox L−1) ranked as Alicante > Shiraz > Sultaniye > Carignan > Merlot > Chardonnay by CUPRAC, and Shiraz > Alicante > Merlot > Carignan ≥ Sultaniye >  Chardonnay by ABTS/HRP and for skin extracts (mmol Trolox g−1), Carignan > Alicante > Shiraz > Merlot > Chardonnay > Sultaniye by CUPRAC, and Carignan > Shiraz > Alicante > Merlot > Chardonnay >  Sultaniye by ABTS/HRP. The study demonstrated a strong correlation between phenolic composition and TAC values in grape samples, with anthocyanins as the predominant family contributing significantly to TAC. Varietal and intra-fruit differences in specific phenolic compounds were found to account for variations in observed TAC levels. Specifically, grape skin samples showed a more robust correlation between antioxidant capacity and total phenolic content (r = 0.986) than grape juices (r = 0.777). These findings underscore the considerable variation in antioxidant potential among grape varieties and emphasize the critical role of phenolic composition in the health-related benefits of grape consumption. [ABSTRACT FROM AUTHOR]

The aim of this study is the determination some quality parameters of grape and mulberry pestils (leathers) sold in Elazig. For this purpose, a total of 45 fruit pestil samples, of which 23 were grape pestils and 22 were mulberry pestils, were analyzed for their physical properties, chemical properties, and microbiological characteristics. In grape and mulberry pestils, the average L* (lightness/darkness) values were 42.72 and 44.81, a* (redness) values were 10.29 and 9.07, and b* (yellow) values were 20.90 and 20.90, respectively. In grape and mulberry fruit pestil samples, the thickness values were 1.62 and 1.34, pH values were 3.82 and 4.75, acidity values (as citric acid) were 0.63 and 0.22%, aw values were 0.602 and 0.627, dry matter values were 68.78% and 72.99 %, ash values were 2.25% and 0.64%, insoluble ash values in 10% HCl were 0.20 and 0.29, and Brix values were 18.83 and 19.36, respectively. TMAB, coliform, Staphylococcus-Micrococcus, and yeast-mold counts were found to be 4.72 and 4.48, 1.30 and 1.89, 2.11 and 2.12, and 2.04 and 2.11 log10 cfu/g, respectively, in all samples examined. In 14 of the samples (31.11%), the total number of mesophilic aerobic microorganisms was found to be >5 log10 cfu/g. Escherichia coli and Salmonella spp. were not found in any of the samples. However, the samples were found to contain significant levels of yeast-mold. There was no difference between the groups in microbiological terms in statistical analysis (P > 0.05). According to the current study, there was no standard production and not enough hygiene rules were observed in the pestil production process in family businesses in Elazig. On the other hand, essential information was obtained on some physical, chemical and microbiological quality parameters of grape and blackberry pulps sold in markets and stores in Elazig. [ABSTRACT FROM AUTHOR]

In recent years, an increase in the incidence of liver diseases has been reported all over the world. This study aims to comprehensively summarize and quantitatively analyze the existing evidence concerning the effectiveness of grape‐derived products on liver enzymes through a systematic review and meta‐analytic approach. PubMed, Scopus, Cochrane Library, and ISI Web of Science were comprehensively searched until January 2024. Articles that reported the effect of grape‐derived products on serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) levels were included. Weighted mean differences (WMDs) were pooled using a random‐effects model. Nine studies were included in the meta‐analysis. The results revealed that grape‐derived products did not significantly change the concentrations of ALT (WMD: −2.70 IU/L, 95% CI: −6.14 to 0.75, p = 0.12), and AST (WMD: ‐1.42 IU/L, 95% CI: −3.54 to 0.70, p = 0.18). However, a significant reduction was observed in serum ALP levels (WMD: ‐5.49 IU/L, 95% CI: −9.57 to −1.4, p = 0.008). The present findings suggest that grape‐derived products positively influence serum ALP levels among adults. However, a more comprehensive decision necessitates additional studies. [ABSTRACT FROM AUTHOR]

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Grapes (Vitis vinifera L.) are known for their deliciousness and health benefits. In the present study, the variations of physicochemical profiles and antioxidant activities of 25 grape varieties were evaluated. Among the varieties tested, glucose and fructose were the main sugars, and tartaric and malic acids were the main organic acids in all grapes. Shine Muscat was characterized as suitable for fresh eating due to its high sugar contents and low acid contents. While JintianMeigui had high sugar and anthocyanins contents, making it suitable for cultivation. Results showed that all grape fractions are sources of phenolic compounds and varied among cultivars. In general, regardless of cultivars, seed extracts showed the highest content of total phenolics and total proanthocyanidins contents, consistent with the observed higher antioxidant activities. Combining high-performance liquid chromatography (HPLC) and high-resolution tandem mass spectrometry (HRMS) technologies, a total of 28 phenolic components were preliminarily identified. The most abundant anthocyanins in skins were petunidin-3-glucoside and malvidin-3-glucoside, as well as catechin and epicatechin were the principal proanthocyanidins in seeds. Pearson correlation coefficient analysis showed the significant linear correlations between proanthocyanidins (catechin, epicatechin, proanthocyanidin A1, proanthocyanidin B1, proanthocyanidin B2, proanthocyanidin C1 and epicatechin-3-O-gallate) and antioxidant activities. However, further studies are needed to demonstrate and elucidate the health benefits of grapes for human nutrition as antioxidants. Overall, our results provided theoretical guidance for the further development and utilization of grapes. [ABSTRACT FROM AUTHOR]

In order to explore the application value of different grape varieties in fruit beer brewing and their effects on beer quality, we studied the effects of addition of different levels (30% and 40%, V/V) of Syrah or Merlot grape mashes on the aroma, physical and chemical indexes and antioxidant activity of fruit beer. Besides, grape beer with better quality was determined by sensory evaluation. The results showed that both grape varieties increased the concentration and antioxidant properties of phenols including 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity, ferric and cupric ion reducing power abilities, and significantly elevated the acidity and alcohol concentration of beer. The beer made with the addition of 30% Syrah or 40% (V/V) Merlot exhibited the highest content of aroma compounds, which increased by 190.55% and 152.65%, respectively, when compared with the control group. Sensory evaluation showed that the beer made with the addition of 30% Syrah tasted best. In general, the addition of different varieties of grape mashes had a positive effect on improving beer quality and aroma, which could become an important direction for the development of diversified beer products in the future.

Exogenous gibberellic acid was applied to regulate fruit growth of grapes to acquire high quality seedless berries. The vascular bundles provide the pathway of water and nutrient transport into the fruit and play an important role in berry growth and sugar accumulation. Therefore, changes in berry size and quality are closely related to the structure and function of the vascular tissue. In this study, 10-year-old “Shine Muscat” grape vines were treated with GA3 in grapevine production. Based on previous research and experimental environmental conditions, four treatments were applied: elongating cluster（5 mg L−1 GA3 on Apr. 28）+ seedless（25 mg L−1 GA3 on May. 24）+ expanding（50 mg L−1 GA3 on Jun. 7） (T1), seedless（25 mg L−1 GA3 on May. 24）+ expanding（50 mg L−1 GA3 on Jun. 7） (T2), expanding（50 mg L−1 GA3 on Jun. 7） (T3) and water (CK). Compared to CK, all fruits treated with GA3 had greater berry size. In addition, GA3 treated grapes had greater vascular bundle areas, including the areas of both phloem and xylem, as well as a greater number of vessels compared to control fruit. During the first rapid growth stage, water transport into the grape was rapid and did not differ among treatments. During veraison, the water transport rate among the four treatments differed, and the rank of speed was T2>T3>CK>T1. Therefore, we found that GA3 treatment can significantly promote the differentiation of vascular tissues and improve water transport capacity and phloem sugar unloading; T2 promoted the development of vascular bundles, enhanced water transport speed and phloem sugar unloading of fruit after veraison, and increased berry size.

ABSTRACT: To effectively assess the extent of the mycotoxin contamination of grapes, a method was developed to analyze 22 mycotoxins by ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). Samples were extracted and purified using the QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure. The extracts were separated on a BEH Shield C18 column (100 mm × 2.1 mm, 1.7 μm), using methanol and water with 0.1% formic acid solution and 2 mmol/L ammonium acetate as the mobile phases. The quantification was performed according to a matrix-matched external standard method. There was a good linear relationship within the respective mass concentration ranges of 22 mycotoxins; the correlation coefficients were not less than 0.998 1. The limit of quantification was 0.3–10.0 μg/kg. Mycotoxin recovery ranged from 68.6% to 109.0%, with a relative standard deviation of 1.28%–12.80%. The method is simple, rapid, highly sensitive, and accurate, making it useful for screening grapes for common and emerging mycotoxins.

Purpose: T 2$$ {}_2 $$‐weighted turbo‐spin‐echo (TSE) sequences are a fundamental technique in brain imaging but suffer from field inhomogeneities at ultra‐high fields. Several methods have been proposed to mitigate the problem, but were limited so far to nonselective three‐dimensional (3D) measurements, making short acquisitions difficult to achieve when targeting very high resolution images, or needed additional calibration procedures, thus complicating their application. Methods: Slab‐selective excitation pulses were designed for flexible placement utilizing the concept of k T$$ {}_T $$‐spokes. Phase‐coherent refocusing universal pulses were subsequently optimized with the Gradient Ascent Pulse Engineering algorithm and tested in vivo for improved signal homogeneity. Results: Implemented within a 3D variable flip angle TSE sequence, these pulses led to a signal‐to‐noise ratio (SNR) improvement ranging from 10% to 30% compared to a two‐dimensional (2D) T2w TSE sequence employing B1+$$ {\mathrm{B}}_1^{+} $$‐shimmed pulses. B1+$$ {\mathrm{B}}_1^{+} $$ field inhomogeneities could be mitigated and artifacts from B0$$ {\mathrm{B}}_0 $$ deviations reduced. The concept of universal pulses was successfully applied. Conclusion: We present a pulse design method which provides a set of calibration‐free universal pulses (UPs) for slab‐selective excitation and phase‐coherent refocusing in slab‐selective TSE sequences. [ABSTRACT FROM AUTHOR]

This investigation was performed during the 2018 and 2019 seasons in a private vineyard to study the effect of calcium, magnesium fertigation and molybdenum foliar sprays on the yield and fruit quality of Flame seedless table grape cultivar. The experimental layout was arranged in a split-plot design. All treatments were applied on three dates: after the bud burst stage, at the fruit set stage and veraison stage. The results showed that the combined application of 30 Kg calcium with 20 Kg magnesium fertigation and foliar spraying of 30 ppm molybdenum was the most effective among all treatments in terms of enhancing vegetative growth characteristics, increasing leaf content of total chlorophylls and mineral elements as well as improving yield and fruit quality attributes.

Abstract Background Monoterpenes are among the most important volatile aromatic compounds contributing to the flavor and aroma of grapes and wine. However, the molecular basis of monoterpene biosynthesis has not yet been fully elucidated. Results In our study, transcriptomics and gas chromatography-mass spectrometry (GC-MS) were used to mine candidate genes and transcription factors involved in monoterpene biosynthesis between high-monoterpene and zero-monoterpene table grape cultivars. We found that monoterpene biosynthesis was positively correlated by the expression of five genes encoding 1-deoxy-D-xylulose-5-phosphate synthase (VvDXSs), one encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (VvHDR), three hydroxy-3-methylglutaryl-CoA synthases (VvHMGSs) and one mevalonate kinase (VvMVK), whereas the expression of one isopentenyl diphosphate isomerase (VvIDI) and one 3-hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) negatively correlated monoterpene biosynthesis. Of these genes, VvIDI was selected to validate its function in monoterpene accumulation through a transient overexpression experiment, and was shown to inhibit the biosynthesis of grape linalool and α-terpineol. Meanwhile, we found that a 64-amino acid extension sequence at the N-terminus can guide the VvIDI protein to target the chloroplast. Conclusions The findings of this study should help to guide future functional analysis of key genes as well as mining the potential regulatory mechanism of monoterpene biosynthesis in grapes and grape products.

[ Objective ] Grape is an important cash crop in China , and aroma is the major component of the grape fruit quality. Grape aroma is complex and composed of a variety of compounds , including terpenes , volatile aliphatic compounds , aromatic compounds , pyrazines , and sulfur-containing compounds. It is af- fected by multiple factors , where genetic regulation is the main factor affecting its aroma. Therefore , ana- lyzing genetic regulation of grape aroma production provides a basis for achieving breeding goals. [ Reviews ] This paper reviewed the methods of grape aroma measurement , followed by summarizing and analyzing the genetic regulation and QTL mapping of grape aroma traits. [ Prospect ] This paper laid a the- oretical foundation for analyzing genetic regulation of grape aroma and provided a reference for grape aroma trait- directed breeding.

Abstract To alleviate the selenium (Se) stress in fruit trees and improve its accumulation, the effects of exogenous indole-3-acetic acid (IAA) on the growth and Se accumulation of grapevine under Se stress were studied. The application of exogenous IAA increased the biomass of grapevine, and the concentration of exogenous IAA had a regression relationship with the biomass. The root and shoot biomass were the maximum at 60 mg L− 1 IAA, increasing by 15.61% and 23.95%, respectively, compared with the control. Exogenous IAA also increased the photosynthetic pigments and the activities of superoxide dismutase and peroxidase in grapevine. Moreover, exogenous IAA increased the contents of total Se, organic Se, and inorganic Se, and the concentration of exogenous IAA had a regression relationship with the total Se content. The highest contents of root total Se and shoot total Se were accumulated at 90 mg L− 1 IAA, increasing by 29.94% and 55.77% respectively,. In addition, the correlation and path analyses revealed that the carotenoid content and root total Se content were closely associated with the shoot total Se content. Therefore, the application of exogenous IAA can alleviate the stress of Se to grape and promote its uptake and the most effective amount for the uptake of Se is 90 mg L− 1 IAA.

During the storage and transportation of grapes after picking, diseases occurs generally, including gray mold, aspergillus rot, rhizopus rot, penicillium rot, etc., which seriously affect its production and commodity value. At present, the control of postharvest diseases of grape still depends on chemical control. Long-term application of chemical fungicides can lead to pesticide residues, environmental pollution, harm to human health, and enhance the drug resistance of pathogenic fungal. Using antagonistic bacteria to control postharvest diseases of grape is a green, environmental and safe method instead of chemical fungicides. In this paper, the postharvest infection diseases and symptoms of grape, the source, application effect and their mechanism of action of antagonistic bacteria, the combination of antagonists with other methods to enhance the biocontrol effect are introduced. The prospect of controlling postharvest diseases of grape by antagonistic bacteria is also discussed.

Abstract [Objective] Tonoplast sugar transporters (TSTs or TMTs) are crucial sugar transport proteins in plant development. The study aims to investigate the role of this gene family in grape growth and development, to provide a foundation for understanding the function of TST genes. [Methods] A total of 13 TST genes were identified from the grape genome using homology analysis. Gene structures and encoded proteins were analyzed using bioinformatics tools. Utilizing the qRT-PCR technique, TST expression levels in different tissues during ‘Yinhong’ grape development were analyzed, and a correlation with soluble sugar contents in the grapes at different time points were studied. [Results] The TST genes was distributed across the six chromosomes and contained three segmental repetitions and three tandem repetition genes. It could be classified into three subfamilies, each with members sharing structural similarity. TST genes included ample cis regulatory elements related to phytohormone, light, and stress response. Protein family was composed of α-helices and irregular coils, and the protein model of each subfamily was similar. VvTST was expressed in various tissues, possessing spatiotemporal expression specificity. Correlation analysis found that the expression levels of four VvTSTs, including VIT _18s0001g12560, VIT _ 18s0122g00850, VIT_04s0023g01860, and VIT_03s0038g03940, exhibited similar trends with the accumulation of soluble sugars in the grape flesh. [Conclusion] VvTST may play a vital role in soluble sugar accumulation in grape flesh.

To investigate the effects of different crop loads on the overall quality of 'Shine Muscat', this study was conducted to regulate the crop loads by berry thinning and to evaluate the sugar, acid and phenolic contents, antioxidant activity and economic benefits of the berries. The results showed that the average berry weight at low crop loads increased significantly, glucose and fructose contents increased significantly, and tartaric and malic acid contents decreased significantly, leading to a significant increase in the sugar-acid ratio of the berries (P

Climate change is increasing the frequency and severity of drought strain, which poses a first-rate task to grapevine production. This have a look at investigated the response of four grape rootstocks (Richter, Salt Creek, Freedom, and Dogridge) to water deficit prompted by way of polyethylene glycol (PEG). Two pressure induction techniques had been as compared: surprise remedy, related to direct exposure of cultures to growing PEG concentrations (0%, 2%, 4%, 6%, 8%, and 10%); and step-clever long-time period remedy, steadily increasing PEG concentrations (0%, 6%, 8%, and 10%) through the years. The essential findings were as follows: drought strain negatively impacted all rootstocks, leading to decreased morphological tendencies (shoot number, period, and root number), survival %, and biochemical parameters (chlorophyll a and b, carotenoids, stomata popularity, RWC content material). It additionally led to reduced nutrient accumulation (N, P, K, Mg, Ca) in leaves. However, all rootstocks exhibited increased Proline content and antioxidant enzyme hobby under all PEG concentrations. In phrases of rootstock-unique responses, Richter and Salt Creek showed the maximum sturdy performance, maintaining better shoot and root growth, nutrient content, and photosynthetic hobby compared to Freedom and Dogridge. On the alternative hand, Freedom and Dogridge exhibited extra sensitivity to drought stress, experiencing stronger discounts in boom, biochemical parameters, and nutrient accumulation. In conclusion Richter and Salt Creek rootstocks could be valuable equipment for reinforcing drought tolerance in grapevines. The two carried out PEG remedies provide valuable methods for screening and deciding on drought-tolerant grape rootstocks.

Background: Monoterpenes are among the most important volatile aromatic compounds contributing to the flavor and aroma of grapes and wine. However, the molecular basis of monoterpene biosynthesis has not yet been fully elucidated. Results: In our study, transcriptomics and gas chromatography-mass spectrometry (GC-MS) were used to mine candidate genes and transcription factors involved in monoterpene biosynthesis between high-monoterpene and zero-monoterpene table grape cultivars. We found that monoterpene biosynthesis was positively correlated by the expression of five genes encoding 1-deoxy-D-xylulose-5-phosphate synthase (VvDXSs), one encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (VvHDR), three hydroxy-3-methylglutaryl-CoA synthases (VvHMGSs) and one mevalonate kinase (VvMVK), whereas the expression of one isopentenyl diphosphate isomerase (VvIDI) and one 3-hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) negatively correlated monoterpene biosynthesis. Of these genes, VvIDI was selected to validate its function in monoterpene accumulation through a transient overexpression experiment, and was shown to inhibit the biosynthesis of grape linalool and α-terpineol. Meanwhile, we found that a 64-amino acid extension sequence at the N-terminus can guide the VvIDI protein to target the chloroplast. Conclusions: The findings of this study should help to guide future functional analysis of key genes as well as mining the potential regulatory mechanism of monoterpene biosynthesis in grapes and grape products. [ABSTRACT FROM AUTHOR]

BACKGROUND RESULTS CONCLUSION Viticulture has adapted foliar applications of biostimulants as a tool to improve crop quality. Recently, nanotechnology has been incorporated as a strategy to reduce the loss of biostimulants and treat nutrient deficiencies. Therefore, the present study aimed to investigate the effect of foliar applications of amorphous calcium phosphate nanoparticles (ACP) doped with methyl jasmonate (ACP‐MeJA) and urea (ACP‐Ur), individually or together (ACP‐MeJA+Ur), on the content of volatile compounds in ‘Tempranillo’ grapes, compared to the conventional application of MeJA and Ur, individually or in combination (MeJA+Ur).The results showed that nanoparticle treatments reduced the total C6 compounds and some carbonyl compounds in the grape musts. This is of novel interest because their presence at high levels is undesirable to quality. In addition, some aroma‐positive compounds such as nerol, neral, geranyl acetone, β‐cyclocitral, β‐ionone, 2‐phenylethanal and 2‐phenylethanol increased, despite applying MeJA and Ur at a lower dose.Consequently, although few differences in grape volatile composition were detected, nanotechnology could be an option for improving the aromatic quality of grapes, at the same time as reducing the required doses of biostimulants and generating more sustainable agricultural practices. © 2024 The Author(s). Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Anthocyanins and proanthocyanidins are considered to be essential secondary metabolites in grapes and are used to regulate metabolic processes, while miRNAs are involved in their synthesis of anthocyanins and proanthocyanidins to regulate metabolic processes. The present research work was carried out to investigate the underlying regulatory mechanism of target genes in the grape cultivars 'Italia' and 'Benitaka'. miRNA and transnscriptomic sequencing technology were employed to characterize both the profiles of miRNAs and the transcripts of grape peels at 10 and 11 weeks post flowering (10 wpf and 11 wpf). The results revealed that the expression level of vvi-miR828a in 'Italia' at 10 and 11 wpf was significantly higher than that in 'Benitaka'. miRNA-seq analysis predicted MYBPA1 to be the target gene of vvi-miR828a. In transcriptome analysis, the expression level of the VvMYBPA1 gene in 'Benitaka' was significantly higher than that in 'Italia'; in addition, the TPM values (expression levels) of VvMYBPA1 and miR828a also showed an evident negative correlation. The determination of the proanthocyanidin (PA) content in 'Italia' and 'Benitaka' peels at 11 wpf demonstrated that the PA content of 'Benitaka' was significantly higher than that of 'Italia'. The outcomes of RT-qRCR analysis exhibited that the expression levels of the VdPAL, VdCHS, VdCHI, VdDFR, VdMYB5b, VdANR, and VdMYBPA1 genes related anthocyanin and proanthocyanidin pathways were reduced, while the expression levels of all of the above genes were increased after the transient expression of the VvMYBPA1 vector into grape leaves. The results of the transient overexpression experiment of vvi-miR828a before the veraison period of strawberry fruits showed that vvi-miR828a can significantly slow down the coloration of strawberries. The vvi-miR828a negatively regulates the accumulation of proanthocyanidins in grape fruits by inhibiting the expression of VvMYBPA1. [ABSTRACT FROM AUTHOR]

This study investigates the extraction and isolation of cellulose nanocrystals (CNCs) from grape pomace, an agricultural waste material abundant in phenolic compounds. The process involves organic isolation, alkaline bleaching at various concentrations, and acid hydrolysis to obtain pure cellulose and CNCs. Structural analyses using XRD, FTIR, and TGA reveal critical insights. Comparison with existing research on CNCs derived from other biomass sources, such as Siam weed, highlights similarities in the preparation method and structural confirmation through FTIR analysis. TGA results demonstrate the higher initial decomposition temperature of cellulose from grape pomace compared to unprocessed pomace, showcasing improved thermal stability after chemical purification. In TGA analysis because of high crystal content, 3% NaOH/H2O2 alkaline isolation exhibits the lowest water weight loss among all samples. Observations on crystallinity index variations in CNCs attribute these changes to alterations in alkali isolation concentration, corroborated by previous studies. The success of organic and alkaline isolations underscores the effectiveness of these methods in obtaining cellulose from grape pomace. The obtained cellulose and CNCs exhibit high potential in biomaterial applications. This study elevates the value of grape pomace from a disregarded waste product to a valuable source of cellulose for diverse applications in biomaterials, food, and bioprocess. Furthermore, the exploration of CNCs from grape pomace in natural packaging coatings and drug carrier systems opens new avenues for their utilization. In conclusion, the study emphasizes the overlooked potential of grape pomace and establishes the significance of cellulose extracted from this source, suggesting a promising future in various industries. [ABSTRACT FROM AUTHOR]

Key message: The VaBAM3 cloned from Vitis amurensis can enhance the cold tolerance of overexpressed plants, but VaBAM3 knock out by CRISPR/Cas9 system weakened grape callus cold tolerance. In grape production, extreme cold conditions can seriously threaten plant survival and fruit quality. Regulation of starch content by β-amylase (BAM, EC: 3.2.1.2) contributes to cold tolerance in plants. In this study, we cloned the VaBAM3 gene from an extremely cold-tolerant grape, Vitis amurensis, and overexpressed it in tomato and Arabidopsis plants, as well as in grape callus for functional characterization. After exposure to cold stress, leaf wilting in the VaBAM3-overexpressing tomato plants was slightly less pronounced than that in wild-type tomato plants, and these plants were characterized by a significant accumulation of autophagosomes. Additionally, the VaBAM3-overexpressing Arabidopsis plants had a higher freezing tolerance than the wild-type counterparts. Under cold stress conditions, the activities of total amylase, BAM, peroxidase, superoxide dismutase, and catalase in VaBAM3-overexpressing plants were significantly higher than those in the corresponding wild-type plants. Furthermore, sucrose, glucose, and fructose contents in these lines were similarly significantly higher, whereas starch contents were reduced in comparison to the levels in the wild-type plants. Furthermore, we detected high CBF and COR gene expression levels in cold-stressed VaBAM3-overexpressing plants. Compared with those in VaBAM3-overexpressing grape callus, the aforementioned indicators tended to change in the opposite direction in grape callus with silenced VaBAM3. Collectively, our findings indicate that heterologous overexpression of VaBAM3 enhanced cold tolerance of plants by promoting the accumulation of soluble sugars and scavenging of excessive reactive oxygen species. These findings provide a theoretical basis for the cultivation of cold-resistant grape and support creation of germplasm resources for this purpose. [ABSTRACT FROM AUTHOR]

Grape (Vitis vinifera L.) is an important fruit crop and is widely cultivated because of its nutritional and economical values. The foliar application of nutrients improves the performance and quality of grapes, and silicon is recently classified as an essential element for plants. For this purpose, a factorial experiment was conducted in a complete randomized design with three replications on grapes cv. ‘Fakhri’. The treatments included three levels of potassium silicate (0.0, 1.0 (S.K1), and 2.0 g/L (S.K2)), combined with three levels of humic acid (0.0, 1.0 (H1) and 2.0 (H2) g/L). Morphological, physiological and biochemical characteristics, as well as the microelements of the leaves were measured. The highest yield (10.56 kg/vine) was obtained by simultaneous treatment with S.K2 and H2 solutions. The interactive effects of these two treatments changed the activity of catalase, peroxidase and superoxide dismutase and increased the content of zinc, manganese, iron and copper elements in grape leaves. This result indicates that potassium silicate and humic acid had a synergistic effect and increased all quantitative and qualitative indices of ‘Fakhri’ grapes studied in this experiment. [ABSTRACT FROM AUTHOR]

The molecular mechanism of flower development and sexual differentiation, which is an intricate process, still unclear in Vitis plants. In this study, we performed the transcriptome analysis by high-throughput sequencing to measure gene expression at three stages in male, female, and hermaphrodite grape flowers, and in combination with the fluctuation of hormones (ABA, IAA, BR, GA3, and JA) to understand the gene function and molecular basis of developmental processes in Vitis flowers. The results showed that more than 581 million clean reads were generated from all samples and average 83% reads were mapped on the Vitis vinifera genome. More, quite a large number of genes were differentially expressed between different stages within and across cultivars and various transcription factor families involved were identified in flower development. The genes belonging to MYB, bHLH, ERF, NAC, and WRKY have more numbers than other TF families. Furthermore, we analyzed the correlation of those hormones with 165 DEGs (including 36 TF genes). The results suggested that those genes might play important roles in sex differentiation during flower development and a unique opportunity to increase the efficiency of crossbreeding in Vitis plant. [ABSTRACT FROM AUTHOR]

In this study, the effect of vine training system and storage time were evaluated on some grape characteristics, such as berry contamination percentage, berry shedding percentage, berry browning rate, titratable acidity, soluble solids, total phenol and the activity of peroxidase, polyphenol oxidase and phenylalanine ammonia -lyase enzymes, in order to maintain the quality of grape during storage time. Factorial experiment was conducted based on completely randomized design with two factors of vine training system (Khazandeh, pergola and cordon) and storage time (zero, 20, 40 and 60 days after storage) with three replications during 2018 -2019. After transferring the fruits to the cold storage with temperature of +4 ◦C, relative humidity of 85 -90% and storage for two months, some characteristics of grape were examined on different days after storage. The results showed that the level of contamination of the berry increased over time, and during the storage period, the lowest level of contamination (13.706%) was related to the cordon training system. Also, after 60 days of storage, the lowest percentage of berry drop (27.535%) was observed in the fruits harvested from the cordon training system. The amount of berry browning after 60 days of storage was significantly higher than other times. The highest amount of TSS (26.182 degrees Brix) was related to the Khazandeh training system in 40 days after storage, however, a significant decrease in the value of this index was observed during 60 days after storage. The results showed that the amount of phenol increased until the end of the storage period, and the maximum amount of phenol in all three training systems was significantly higher than other days in 60 days after storage. The level of peroxidase enzyme activity in the Khazandeh training system reached its highest level at 20 days after storage, while in the other two training systems, it was significantly higher than the other days at 40 days after storage. Also, the activity of polyphenol oxidase enzyme reached its maximum level in 40 days after storage in Khazandeh and pergola training systems and then decreased. While in cordon training system, the highest activity of this enzyme was observed after 60 days of storage. The activity of phenylalanine ammonia -lyase enzyme increased during the storage period, and 60 days after storage, the highest activity of this enzyme was related to the pergola training system. In general, the results showed that the cordon training system had the best effect in maintaining the characteristics of grape during the storage period compared to other training methods. [ABSTRACT FROM AUTHOR]

WRKYs are a multigenic family of transcription factors that are plant-specific and involved in the regulation of plant development and various stress response processes. However, the evolution of WRKY genes is not fully understood. This family has also been incompletely studied in grapevine, and WRKY genes have been named with different numbers in different studies, leading to great confusion. In this work, 62 Vitis vinifera WRKY genes were identified based on six genomes of different cultivars. All WRKY genes were numbered according to their chromosomal location, and a complete revision of the numbering was performed. Amino acid variability between different cultivars was assessed for the first time and was greater than 5% for some WRKYs. According to the gene structure, all WRKYs could be divided into two groups: more exons/long length and fewer exons/short length. For the first time, some chimeric WRKY genes were found in grapevine, which may play a specific role in the regulation of different processes: VvWRKY17 (an N-terminal signal peptide region followed by a non-cytoplasmic domain) and VvWRKY61 (Frigida-like domain). Five phylogenetic clades A–E were revealed and correlated with the WRKY groups (I, II, III). The evolution of WRKY was studied, and we proposed a WRKY evolution model where there were two dynamic phases of complexity and simplification in the evolution of WRKY. [ABSTRACT FROM AUTHOR]

This investigation was performed during the 2018 and 2019 seasons in a private vineyard to study the effect of calcium, magnesium fertigation and molybdenum foliar sprays on the yield and fruit quality of Flame seedless table grape cultivar. The experimental layout was arranged in a split-plot design. All treatments were applied on three dates: after the bud burst stage, at the fruit set stage and veraison stage. The results showed that the combined application of 30 Kg calcium with 20 Kg magnesium fertigation and foliar spraying of 30 ppm molybdenum was the most effective among all treatments in terms of enhancing vegetative growth characteristics, increasing leaf content of total chlorophylls and mineral elements as well as improving yield and fruit quality attributes. [ABSTRACT FROM AUTHOR]