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7. A Point Mutation V419L in the Sodium Channel Gene from Natural Populations of Aedes aegypti Is Involved in Resistance to λ-Cyhalothrin in Colombia

Author

Granada, Yurany, Mejía-Jaramillo, Ana María, STRODE, CLARE, Triana-Chavez, Omar, Granada, Yurany, Mejía-Jaramillo, Ana María, STRODE, CLARE, and Triana-Chavez, Omar

Abstract

Resistance to pyrethroids in mosquitoes is mainly caused by target site insensitivity known as knockdown resistance (kdr). In this work, we examined the point mutations present in portions of domains I, II, III, and IV of the sodium channel gene in Aedes aegypti mosquitoes from three Colombian municipalities. A partial region coding for the sodium channel gene from resistant mosquitoes was sequenced, and a simple allele-specific PCR-based assay (AS-PCR) was used to analyze mutations at the population level. The previously reported mutations, V1016I and F1534C, were found with frequencies ranging from 0.04 to 0.41, and 0.56 to 0.71, respectively, in the three cities. Moreover, a novel mutation, at 419 codon (V419L), was found in Ae. aegypti populations from Bello, Riohacha and Villavicencio cities with allelic frequencies of 0.06, 0.36, and 0.46, respectively. Interestingly, the insecticide susceptibility assays showed that mosquitoes from Bello were susceptible to λ-cyhalothrin pyrethroid whilst those from Riohacha and Villavicencio were resistant. A positive association between V419L and V1016I mutations with λ-cyhalothrin resistance was established in Riohacha and Villavicencio. The frequency of the F1534C was high in the three populations, suggesting that this mutation could be conferring resistance to insecticides other than λ-cyhalothrin, particularly type I pyrethroids. Further studies are required to confirm this hypothesis.

Published
2018

9. Epidemiological features of Leishmania infantum in dogs (Canis lupus familiaris) suggest a latent risk of visceral leishmaniasis in the metropolitan area of Bucaramanga‚ Santander.

Author

Jaimes Dueñez, Jeiczon, Castillo Castañeda, Adriana, Jiménez Leaño, Ángela, Duque, Jonny E., Cantillo-Barraza, Omar, Cáceres-Rivera, Diana I., Granada, Yurany, Triana-Chávez, Omar, and David Ramírez, Juan

Subjects
DOGS, VISCERAL leishmaniasis, SAND flies, LEISHMANIA infantum, METROPOLITAN areas, CITIES & towns
Abstract

Introduction: this study aims to investigate the eco-epidemiological factors associated with Leishmania spp. infection in dogs from the Metropolitan Area of Bucaramanga (MAB)‚ Santander‚ eastern Colombia‚ which is a region at risk of VL. Methods: we conducted molecular and serological surveillance of Leishmania spp. in 207 dogs from MAB to determine the epidemiological factors associated with infection. Subsequently‚ we carried out a molecular and serological analysis of phlebotomine and humans in the area to describe the main features associated with the transmission cycle. Results: out of the 207 dogs tested‚ 37 (17.8 %‚ 95 % CI=12.6 - 23.1 %) were positive for Leishmania spp. by the IFAT test‚ and only 9 (4.3 %‚ 95 % CI=1.55 - 7.15 %) were positive for L. infantum in the molecular analysis. Multivariate analyses indicated that canine shelters had a higher prevalence of infection (p < 0.05). In the entomological survey‚ 69 blood-fed female phlebotomine of the genus Lutzomyia were captured in canine shelters‚ among them‚ 55 % were identified as Lutzomyia camposi‚ 29 % as Lu. ovallesi‚ 7 % as Lu. dubitans‚ 6 % as Lu. torvida‚ and 3 % as Lu. cayennensis. The identified meal sources of the phlebotomine included human‚ pig‚ avian‚ cattle‚ and porcupine (Coendou quichua) blood. However‚ no phlebotomine positive for Leishmania spp. were detected by molecular analyses. Finally‚ 14 humans who had frequent contact with L. infantum-positive dogs were analyzed through rK39 test‚ but none was positive for IgG/IgM antibodies. Conclusion: the molecular and serological analyses indicate a low prevalence of L. infantum in dogs from MAB‚ with canine shelters having the highest prevalence of infection. The entomological survey of canine shelters showed a significant diversity of phlebotomine without potential vectors of L. infantum‚ suggesting that the infection in dogs from these areas could take place in other locations or through other transmission routes. The circulation of L. infantum in multiple dogs from MAB suggests a latent risk of zoonotic transmission of VL in these cities. [ABSTRACT FROM AUTHOR]

Published
2024

10. Mutaciones en el gen del canal de sodio en poblaciones colombianas de Aedes aegypti asociadas con el desarrollo de resistencia a insectidicidas.

Author

Granada, Yurany, María Mejía, Ana, and Triana, Omar

Abstract

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2022

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