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19 results on '"Graham, Madeline"'

9. Differential effects of Akkermansia-enriched fecal microbiota transplant on energy balance in female mice on high-fat diet

Author

Acharya, Kalpana D., primary, Friedline, Randall H., additional, Ward, Doyle V., additional, Graham, Madeline E., additional, Tauer, Lauren, additional, Zheng, Doris, additional, Hu, Xiaodi, additional, de Vos, Willem M., additional, McCormick, Beth A., additional, Kim, Jason K., additional, and Tetel, Marc J., additional

Published
2022
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11. Differential effects of Akkermansia-enriched fecal microbiota transplant on energy balance in female mice on high-fat diet

Author

Acharya, Kalpana D., Friedline, Randall H., Ward, Doyle V., Graham, Madeline E., Tauer, Lauren, Zheng, Doris, Hu, Xiaodi, de Vos, Willem M., McCormick, Beth A., Kim, Jason K., Tetel, Marc J., Acharya, Kalpana D., Friedline, Randall H., Ward, Doyle V., Graham, Madeline E., Tauer, Lauren, Zheng, Doris, Hu, Xiaodi, de Vos, Willem M., McCormick, Beth A., Kim, Jason K., and Tetel, Marc J.

Abstract

Estrogens protect against weight gain and metabolic disruption in women and female rodents. Aberrations in the gut microbiota composition are linked to obesity and metabolic disorders. Furthermore, estrogen-mediated protection against diet-induced metabolic disruption is associated with modifications in gut microbiota. In this study, we tested if estradiol (E2)-mediated protection against obesity and metabolic disorders in female mice is dependent on gut microbiota. Specifically, we tested if fecal microbiota transplantation (FMT) from E2-treated lean female mice, supplemented with or without Akkermansia muciniphila, prevented high fat diet (HFD)-induced body weight gain, fat mass gain, and hyperglycemia in female recipients. FMT from, and cohousing with, E2-treated lean donors was not sufficient to transfer the metabolic benefits to the E2-deficient female recipients. Moreover, FMT from lean donors supplemented with A. muciniphila exacerbated HFD-induced hyperglycemia in E2-deficient recipients, suggesting its detrimental effect on the metabolic health of E2-deficient female rodents fed a HFD. Given that A. muciniphila attenuates HFD-induced metabolic insults in males, the present findings suggest a sex difference in the impact of this microbe on metabolic health.

Published
2022

19. Central in vivo mechanisms by which C. difficile's proline reductase drives efficient metabolism, growth, and toxin production.

Author

Cersosimo LM, Graham M, Monestier A, Pavao A, Worley JN, Peltier J, Dupuy B, and Bry L

Abstract

Clostridioides difficile (CD) is a sporulating and toxin-producing nosocomial pathogen that opportunistically infects the gut, particularly in patients with depleted microbiota after antibiotic exposure. Metabolically, CD rapidly generates energy and substrates for growth from Stickland fermentations of amino acids, with proline being a preferred reductive substrate. To investigate the in vivo effects of reductive proline metabolism on C. difficile's virulence in an enriched gut nutrient environment, we evaluated wild-type and isogenic ΔprdB strains of ATCC43255 on pathogen behaviors and host outcomes in highly susceptible gnotobiotic mice. Mice infected with the ΔprdB mutant demonstrated extended survival via delayed colonization, growth and toxin production but ultimately succumbed to disease. In vivo transcriptomic analyses demonstrated how the absence of proline reductase activity more broadly disrupted the pathogen's metabolism including failure to recruit oxidative Stickland pathways, ornithine transformations to alanine, and additional pathways generating growth-promoting substrates, contributing to delayed growth, sporulation, and toxin production. Our findings illustrate the central role for proline reductase metabolism to support early stages of C. difficile colonization and subsequent impact on the pathogen's ability to rapidly expand and cause disease.

Published
2023
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