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3. On the solvent-extraction of acetylcholine receptor.

Author

Christopher JP, Millar DB, and Grafius MA

Subjects
Acetylcholine metabolism, Animals, Chloroform, Chromatography, Gel, Electric Organ analysis, Electrophorus, Methanol, Solvents, Receptors, Cholinergic isolation & purification
Abstract

Chloroform/methanol rechromatography on Sephadex LH-20 of fractions isolated from organic solvent extracts of E. electricus electric organs exhibited alterations in elution point compared to the original chromatography. Additionally, 3H decamethonium bromide did not coelute with any of the observed fractions. It is concluded that as presently described the organic solvent isolation procedure for the cholinergic receptor exhibits undesirable characteristics.

Published
1978
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4. High pressure stabilization of acetylcholinesterase sizeozymes against thermal denaturation.

Author

Millar DB, Grafius MA, Wild JR, and Palmer DA

Subjects
Animals, Centrifugation, Density Gradient, Drug Stability, Electrophorus, Hot Temperature, Kinetics, Macromolecular Substances, Mathematics, Molecular Weight, Pancreas enzymology, Pressure, Protein Denaturation, Temperature, Thermodynamics, Time Factors, Trypsin, Acetylcholinesterase isolation & purification, Isoenzymes isolation & purification
Published
1974
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8. A poly I cryoform.

Author

Millar DB and Grafius MA

Subjects
Chemical Phenomena, Chemistry, Physical, Fluorescence, Spectrophotometry, Temperature, Cold Temperature, Polynucleotides
Published
1967
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9. Acetylcholinesterase interaction with a lipoprotein matrix.

Author

Grafius MA, Bond HE, and Millar DB

Subjects
Alkaline Phosphatase, Animals, Binding Sites, Centrifugation, Density Gradient, Centrifugation, Zonal, Chemical Phenomena, Chemistry, Chromatography, Gel, Fishes, Hyaluronoglucosaminidase, Hydrogen-Ion Concentration, Hydrolysis, Lipase, Macromolecular Substances, Models, Chemical, Models, Structural, Neuraminidase, Osmolar Concentration, Pancreas enzymology, Phospholipases, Acetylcholinesterase isolation & purification, Electric Organ enzymology, Lipoproteins
Published
1971
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10. Enzymatically active half-monomers of acetylcholinesterase.

Author

Millar DB, Grafius MA, and Palmer DA

Subjects
Acetylcholine, Ammonium Sulfate, Animals, Centrifugation, Density Gradient, Cholinesterase Inhibitors, Drug Stability, Electrophoresis, Disc, Electrophoresis, Polyacrylamide Gel, Electrophorus metabolism, Hot Temperature, In Vitro Techniques, Macromolecular Substances, Molecular Weight, Peptide Hydrolases, Physostigmine, Sodium Dodecyl Sulfate, Sulfhydryl Compounds, Surface-Active Agents, Trypsin, Vibration, Acetylcholinesterase, Electric Organ enzymology
Published
1973
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