79 results on '"Gräslund S"'
Search Results
2. POS0905 CHARACTERISATION OF SWEDISH MYOSITIS PATIENTS WITH ANTI-MDA5 AUTOANTIBODIES AND CORRELATION OF CLINICAL FEATURES WITH AUTOANTIBODY LEVELS
- Author
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Demirdal, D., primary, Van Gompel, E., additional, Wigren, E., additional, Dastmalchi, M., additional, Horuluoglu, B., additional, Galindo-Feria, A. S., additional, Gräslund, S., additional, Chemin, K., additional, Lundberg, I. E., additional, and Notarnicola, A., additional
- Published
- 2022
- Full Text
- View/download PDF
3. Abundant autoantibody isotypes in idiopathic inflammatory myopathies
- Author
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Preger, C., Notarnicola, A., Hellström, Cecilia, Wigren, E., Lundberg, I. E., Jakobsson, P. J., Persson, Helena, Gräslund, S., Preger, C., Notarnicola, A., Hellström, Cecilia, Wigren, E., Lundberg, I. E., Jakobsson, P. J., Persson, Helena, and Gräslund, S.
- Abstract
QC 20230404
- Published
- 2022
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- View/download PDF
4. OP0321 DELINEATING THE IMMUNOGENIC DOMAINS OF MDA5 USING PATIENT DERIVED AUTOANTIBODIES
- Author
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Van Gompel, E., primary, Cerqueira, C., additional, Wigren, E., additional, Gräslund, S., additional, Chemin, K., additional, Horuluoglu, B., additional, De Langhe, E., additional, Benveniste, O., additional, and Lundberg, I. E., additional
- Published
- 2021
- Full Text
- View/download PDF
5. Generation and validation of recombinant antibodies to study human aminoacyl-tRNA synthetases
- Author
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Preger, C., Wigren, E., Ossipova, E., Marks, C., Lengqvist, J., Hofström, Camilla, Andersson, Oskar, Jakobsson, P. -J, Gräslund, S., Persson, Helena, Preger, C., Wigren, E., Ossipova, E., Marks, C., Lengqvist, J., Hofström, Camilla, Andersson, Oskar, Jakobsson, P. -J, Gräslund, S., and Persson, Helena
- Abstract
Aminoacyl-tRNA synthetases (aaRSs) have long been viewed as mere housekeeping proteins and have therefore often been overlooked in drug discovery. However, recent findings have revealed that many aaRSs have noncanonical functions, and several of the aaRSs have been linked to autoimmune diseases, cancer, and neurological disorders. Deciphering these roles has been challenging because of a lack of tools to enable their study. To help solve this problem, we have generated recombinant high-affinity antibodies for a collection of thirteen cytoplasmic and one mitochondrial aaRSs. Selected domains of these proteins were produced recombinantly in Escherichia coli and used as antigens in phage display selections using a synthetic human single-chain fragment variable library. All targets yielded large sets of antibody candidates that were validated through a panel of binding assays against the purified antigen. Furthermore, the top-performing binders were tested in immunoprecipitation followed by MS for their ability to capture the endogenous protein from mammalian cell lysates. For antibodies targeting individual members of the multi-tRNA synthetase complex, we were able to detect all members of the complex, co-immunoprecipitating with the target, in several cell types. The functionality of a subset of binders for each target was also confirmed using immunofluorescence. The sequences of these proteins have been deposited in publicly available databases and repositories. We anticipate that this open source resource, in the form of high-quality recombinant proteins and antibodies, will accelerate and empower future research of the role of aaRSs in health and disease., QC 20201223
- Published
- 2020
- Full Text
- View/download PDF
6. Serum and balf-derived anti-JO1 autoantibodies exhibit high reactivity to distinct HISRS domains and associate with lung and joint involvement in patients with IIM/ASS
- Author
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Notarnicola, A., Preger, C., Lundström, S., Renard, N., Wigren, E., Van Gompel, E., Galindo-Feria, A. S., Persson, Helena, Fathi, M., Grunewald, J., Jakobsson, P. J., Gräslund, S., Lundberg, I. E., Cerqueira, C., Notarnicola, A., Preger, C., Lundström, S., Renard, N., Wigren, E., Van Gompel, E., Galindo-Feria, A. S., Persson, Helena, Fathi, M., Grunewald, J., Jakobsson, P. J., Gräslund, S., Lundberg, I. E., and Cerqueira, C.
- Abstract
QC 20200825
- Published
- 2020
- Full Text
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7. SAT0335 SERUM AND BALF-DERIVED ANTI-JO1 AUTOANTIBODIES EXHIBIT HIGH REACTIVITY TO DISTINCT HISRS DOMAINS AND ASSOCIATE WITH LUNG AND JOINT INVOLVEMENT IN PATIENTS WITH IIM/ASS
- Author
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Notarnicola, A., primary, Preger, C., additional, Lundström, S., additional, Renard, N., additional, Wigren, E., additional, Van Gompel, E., additional, Galindo-Feria, A. S., additional, Persson, H., additional, Fathi, M., additional, Grunewald, J., additional, Jakobsson, P. J., additional, Gräslund, S., additional, Lundberg, I. E., additional, and Cerqueira, C., additional
- Published
- 2020
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- View/download PDF
8. SAT0288 CHARACTERIZATION OF ANTI-AMINOACYL TRNA SYNTHETASE AUTOANTIBODIES IN PATIENTS WITH IDIOPATHIC INFLAMMATORY MYOPATHIES
- Author
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Preger, C., primary, Notarnicola, A., additional, Hellström, C., additional, Wigren, E., additional, Cerqueira, C., additional, Nilsson, P., additional, Lundberg, I. E., additional, Persson, H., additional, Gräslund, S., additional, and Jakobsson, P. J., additional
- Published
- 2020
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- View/download PDF
9. Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display
- Author
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Pershad, K., Pavlovic, J.D., Gräslund, S., Nilsson, P., Colwill, K., Karatt-Vellatt, A., Schofield, D.J., Dyson, M.R., Pawson, T., Kay, B.K., and McCafferty, J.
- Published
- 2010
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10. A field survey of chemicals and biological products used in shrimp farming
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Gräslund, S, Holmström, K, and Wahlström, A
- Published
- 2003
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11. FRI0409 Myositis specific anti-histidyl trna synthetase (HISRS) autoantibodies display high reactivity against hisrs conformational epitopes and associate with lung and joint involvement
- Author
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Cerqueira, C., primary, Renard, N., additional, Notarnicola, A., additional, Wigren, E., additional, Jakobsson, P.-J., additional, Gräslund, S., additional, and Lundberg, I.E., additional
- Published
- 2018
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12. Antibody validation by immunoprecipitation followed by mass spectrometry analysis
- Author
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Persson, Helena, Preger, C., Marcon, E., Lengqvist, J., Gräslund, S., Persson, Helena, Preger, C., Marcon, E., Lengqvist, J., and Gräslund, S.
- Abstract
We describe a mass spectrometry-based approach for validation of antibody specificity. This method allows validation of antibodies or antibody fragments, against their endogenous targets. It can assess if the antibody is able to bind to its native antigen in cell lysates among thousands of other proteins, DNA, RNA, and other cellular components. In addition, it identifies other proteins the antibody is able to immunoprecipitate allowing for the assessment of antibody specificity and selectivity. This method is easily scalable, adaptable to different cell lines and conditions and has been shown to be reproducible between multiple laboratories., QC 20170523
- Published
- 2017
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13. A pilot project to generate affinity reagents to human proteins
- Author
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Uhlen, M., Gräslund, S., Sundström, M., Uhlen, M., Gräslund, S., and Sundström, M.
- Published
- 2008
14. Structural genomic of protein families and pathways in human disease
- Author
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Weigelt, J., primary, Nordlund, P., additional, Berglund, H., additional, Schuler, H., additional, Holmberg-Schiavone, L., additional, Hällberg, M., additional, Gräslund, S., additional, and Wikström, M., additional
- Published
- 2008
- Full Text
- View/download PDF
15. Single-vector three-frame expression systems for affinity-tagged proteins
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Gräslund, S, primary
- Published
- 2002
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16. Characterisation of the human APC1 , the largest subunit of the anaphase-promoting complex
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Jörgensen, P.M., primary, Gräslund, S., additional, Betz, R., additional, Ståhl, S., additional, Larsson, C., additional, and Höög, C., additional
- Published
- 2001
- Full Text
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17. Feeding aquaculture growth through globalization: exploitation of marine ecosystems for fishmeal
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Deutsch, L, Gräslund, S, Folke, C, Troell, M, Huitric, M, Kautsky, N, Lebel, L, Deutsch, L, Gräslund, S, Folke, C, Troell, M, Huitric, M, Kautsky, N, and Lebel, L
- Abstract
Part of urn:nbn:se:su:diva-232
18. Anti-FHL1 autoantibodies in adult patients with myositis: a longitudinal follow-up analysis.
- Author
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Galindo-Feria AS, Lodin K, Horuluoglu B, Sarrafzadeh-Zargar S, Wigren E, Gräslund S, Danielsson O, Wahren-Herlenius M, Dastmalchi M, and Lundberg IE
- Abstract
Objectives: To determine prevalence and clinical associations of anti-FHL1 autoantibodies in patients with idiopathic inflammatory myopathies (IIM), and to evaluate autoantibody levels over time., Methods: Sera at the time of diagnosis from patients with IIM (n = 449), autoimmune disease controls (DC, n = 130), neuromuscular diseases (NMD, n = 16) and healthy controls (HC, n = 100) were analyzed for anti-FHL1 autoantibodies by Enzyme-Linked ImmunoSorbent Assay (ELISA). Patients with IIM FHL1+ and FHL1- were included in a longitudinal analysis. Serum levels were correlated to disease activity., Results: Autoantibodies to FHL1 were more frequent in patients with IIM (122/449, 27%) compared with DC (Autoimmune DC and NMD, 13/146, 9%, p< 0.001) and HC (3/100,3%, p< 0.001). Anti-FHL1 levels were higher in IIM [median (IQR)=0.62 (0.15-1.04)] in comparison with DC [0.22 (0.08-0.58)], HC [0.35 (0.23-0.47)] and NMD [0.48 (0.36-0.80)] p< 0.001. Anti-FHL1+ patients with IIM were younger at time of diagnosis compared with the anti-FHL1- group (p= 0.05) and were seronegative for other autoantibodies in 25%.In the first follow-up anti-FHL1+ sample 20/33 (60%) positive at baseline had turned negative for anti-FHL1 autoantibodies. Anti-FHL1 autoantibodies rarely appeared after initiating treatment. Anti-FHL1 autoantibody levels correlated with CK (r = 0.62, p= 0.01), disease activity measure MYOACT (n = 14, p= 0.004) and inversely with manual muscle test-8 (r=-0.59, p= 0.02) at baseline., Conclusions: Anti-FHL1 autoantibodies were present in 27% of patients with IIM, of these 25% were negative for other autoantibodies. Other autoimmune diseases had lower frequencies and levels. Anti-FHL1 levels often decreased with immunosuppressive treatment, correlated with disease activity measures at diagnosis and rarely appeared after start of treatment., (© The Author(s) 2024. Published by Oxford University Press on behalf of the British Society for Rheumatology.)
- Published
- 2024
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19. Autoantibodies against the melanoma differentiation-associated protein 5 in patients with dermatomyositis target the helicase domains.
- Author
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Van Gompel E, Demirdal D, Fernandes-Cerqueira C, Horuluoglu B, Galindo-Feria A, Wigren E, Gräslund S, De Langhe E, Benveniste O, Notarnicola A, Chemin K, and Lundberg IE
- Subjects
- Humans, Female, Male, Middle Aged, Adult, Aged, Dermatomyositis immunology, Dermatomyositis blood, Autoantibodies immunology, Autoantibodies blood, Interferon-Induced Helicase, IFIH1 immunology, Enzyme-Linked Immunosorbent Assay
- Abstract
Objectives: Clinical observations in patients with dermatomyositis (DM) and autoantibodies against the melanoma differentiation-associated protein 5 (MDA5) suggest that the autoantibodies contribute to the pathogenesis of MDA5(+) DM. To gain insight into the role of the anti-MDA5 autoantibodies, we aimed to identify their binding sites on the different domains of the MDA5 protein., Methods: We developed an in-house ELISA to assess the reactivity against the MDA5 domains (conformational epitopes) in plasma (n = 8) and serum (n = 24) samples from MDA5(+) patients with varying clinical manifestations and disease outcomes. The reactivities were also assessed using western blot (linearized epitopes). An ELISA-based depletion assay was developed to assess cross-reactivity among the different MDA5 domains., Results: All eight plasma samples consistently showed reactivity towards conformational and linearized epitopes on the helicase domains of the MDA5 protein. The ELISA-based depletion assay suggests that anti-MDA5 autoantibodies specifically target each of the three helicase domains. Twenty-two of the 24 serum samples showed reactivity in the in-house ELISA and all 22 displayed reactivity towards the helicase domains of the MDA5 protein., Conclusions: Our data revealed that the main immunogenic targets of anti-MDA5 autoantibodies from MDA5(+) patients are the helicase domains. Considering that the helicase domains are responsible for the enzymatic activity and subsequent triggering of an inflammatory response, our findings suggest that binding of anti-MDA5 autoantibodies could alter the canonical activity of the MDA5 protein and potentially affect the downstream induction of a pro-inflammatory cascade., (© The Author(s) 2023. Published by Oxford University Press on behalf of the British Society for Rheumatology.)
- Published
- 2024
- Full Text
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20. Autoantigenic properties of the aminoacyl tRNA synthetase family in idiopathic inflammatory myopathies.
- Author
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Preger C, Notarnicola A, Hellström C, Wigren E, Fernandes-Cerqueira C, Kvarnström M, Wahren-Herlenius M, Idborg H, Lundberg IE, Persson H, Gräslund S, and Jakobsson PJ
- Subjects
- Humans, Retrospective Studies, Autoantigens, Autoantibodies, Syndrome, Amino Acyl-tRNA Synthetases, Myositis, Lung Diseases, Interstitial, Arthritis
- Abstract
Objectives: Autoantibodies are thought to play a key role in the pathogenesis of idiopathic inflammatory myopathies (IIM). However, up to 40% of IIM patients, even those with clinical manifestations of anti-synthetase syndrome (ASSD), test seronegative to known myositis-specific autoantibodies. We hypothesized the existence of new potential autoantigens among human cytoplasmic aminoacyl tRNA synthetases (aaRS) in patients with IIM., Methods: Plasma samples from 217 patients with IIM according to 2017 EULAR/ACR criteria, including 50 patients with ASSD, 165 without, and two with unknown ASSD status were identified retrospectively, as well as age and gender-matched sera from 156 population controls, and 219 disease controls. Patients with previously documented ASSD had to test positive for at least one of the five most common anti-aaRS autoantibodies (anti-Jo1, -PL7, -PL12, -EJ, and -OJ) and present with one or more of the following clinical manifestations: interstitial lung disease, myositis, arthritis, Raynaud's phenomenon, fever, or mechanic's hands. Demographics, laboratory, and clinical data of the IIM cohort (ASSD and non-ASSD) were compared. Samples were screened using a multiplex bead array assay for presence of autoantibodies against a panel of 117 recombinant protein variants, representing 33 myositis-related proteins, including all nineteen cytoplasmic aaRS. Prospectively collected clinical data for the IIM cohort were retrieved and compared between groups within the IIM cohort and correlated with the results of the autoantibody screening. Principal component analysis was used to analyze clinical manifestations between ASSD, non-ASSD groups, and individuals with novel anti-aaRS autoantibodies., Results: We identified reactivity towards 16 aaRS in 72 of the 217 IIM patients. Twelve patients displayed reactivity against nine novel aaRS. The novel autoantibody specificities were detected in four previously seronegative patients for myositis-specific autoantibodies and eight with previously detected myositis-specific autoantibodies. IIM individuals with novel anti-aaRS autoantibodies (n = 12) all had signs of myositis, and they had either muscle weakness and/or muscle enzyme elevation, 2/12 had mechanic's hands, 3/12 had interstitial lung disease, and 2/12 had arthritis. The individuals with novel anti-aaRS and a pathological muscle biopsy all presented widespread up-regulation of major histocompatibility complex class I. The reactivities against novel aaRS could be confirmed in ELISA and western blot. Using the multiplex bead array assay, we could confirm previously known reactivities to four of the most common aaRS (Jo1, PL12, PL7, and EJ (n = 45)) and identified patients positive for anti-Zo, -KS, and -HA (n = 10) that were not previously tested. A low frequency of anti-aaRS autoantibodies was also detected in controls., Conclusion: Our results suggest that most, if not all, cytoplasmic aaRS may become autoantigenic. Autoantibodies against new aaRS may be found in plasma of patients previously classified as seronegative with potential high clinical relevance., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2023
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21. Autoantibodies against four-and-a-half-LIM domain 1 (FHL1) in inflammatory myopathies: results from an Australian single-centre cohort.
- Author
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Galindo-Feria AS, Horuluoglu B, Day J, Fernandes-Cerqueira C, Wigren E, Gräslund S, Proudman S, Lundberg IE, and Limaye V
- Subjects
- Australia epidemiology, Autoantigens, Cohort Studies, HLA-DRB1 Chains, Humans, Intracellular Signaling Peptides and Proteins, LIM Domain Proteins, Muscle Proteins, Autoantibodies, Myositis
- Abstract
Objectives: To determine the prevalence and associations of autoantibodies targeting a muscle-specific autoantigen, four-and-a-half-LIM-domain 1 (FHL1), in South Australian patients with histologically-confirmed idiopathic inflammatory myopathies (IIM) and in patients with SSc., Material and Methods: Sera from patients with IIM (n = 267) from the South Australian Myositis Database (SAMD), SSc (n = 174) from the Australian Scleroderma Cohort Study (ASCS) and healthy controls (HC, n = 100) were analysed for anti-FHL1 autoantibodies by Enzyme-Linked ImmunoSorbent Assay (ELISA)., Results: Autoantibodies to FHL1 were more frequent in patients with IIM (37/267, 13.8%) compared with SSc (12/174, 7%) (P < 0.02) and HC (2/100, 2%) (P < 0.001). The most common IIM subtypes among FHL1+ IIM patients were (32%) and IBM (2/37, 32%). No statistically significant differences in muscular or extra-muscular manifestations of IIM were found when comparing patients who were anti-FHL1+ with their anti-FHL1- counterparts. In 29/37 (78%) anti-FHL1+ patients, no myositis-specific autoantibodies (MSA) were present. In FHL1+ muscle biopsies, there was less frequent infiltration by CD45+ cells (P = 0.04). There was a trend for HLA alleles DRB1*07 and DRB1*15 to be more frequent in anti-FHL1+ compared with anti-FHL1- patients (9/25 vs 19/113, P = 0.09 and 8/25 vs 15/114, P = 0.09, respectively)., Conclusions: We report a substantial prevalence (13.8%) of anti-FHL1 autoantibodies in a large cohort of patients with histologically confirmed IIM; 75% of these cases did not have a detectable myositis-specific autoantibody. Anti-FHL1 autoantibodies were also detected in a subgroup of patients with SSc (7%), indicating that anti-FHL1 autoantibodies may not be myositis-specific. The trend towards an HLA-DR association might indicate a specific immune response to the FHL1 protein., (© The Author(s) 2022. Published by Oxford University Press on behalf of the British Society for Rheumatology.)
- Published
- 2022
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22. Longitudinal assessment of reactivity and affinity profile of anti-Jo1 autoantibodies to distinct HisRS domains and a splice variant in a cohort of patients with myositis and anti-synthetase syndrome.
- Author
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Notarnicola A, Preger C, Lundström SL, Renard N, Wigren E, Van Gompel E, Galindo-Feria AS, Persson H, Fathi M, Grunewald J, Jakobsson PJ, Gräslund S, Lundberg IE, and Fernandes-Cerqueira C
- Subjects
- Autoantibodies, Histidine-tRNA Ligase, Humans, Ligases, Lung Diseases, Interstitial, Myositis
- Abstract
Background: To address the reactivity and affinity against histidyl-transfer RNA synthetase (HisRS) autoantigen of anti-Jo1 autoantibodies from serum and bronchoalveolar lavage fluid (BALF) in patients with idiopathic inflammatory myopathies/anti-synthetase syndrome (IIM/ASSD). To investigate the associations between the reactivity profile and clinical data over time., Methods: Samples and clinical data were obtained from (i) 25 anti-Jo1
+ patients (19 sera with 16 longitudinal samples and 6 BALF/matching sera at diagnosis), (ii) 29 anti-Jo1- patients (25 sera and 4 BALF/matching sera at diagnosis), and (iii) 27 age/gender-matched healthy controls (24 sera and 3 BALF/matching sera). Reactivity towards HisRS full-length (HisRS-FL), three HisRS domains (WHEP, antigen binding domain (ABD), and catalytic domain (CD)), and the HisRS splice variant (SV) was tested. Anti-Jo1 IgG reactivity was evaluated by ELISA and western blot using IgG purified from serum by affinity chromatography. In paired serum-BALF, anti-Jo1 IgG and IgA reactivity was analyzed by ELISA. Autoantibody affinity was measured by surface plasmon resonance using IgG purified from sera. Correlations between autoantibody reactivity and clinical data were evaluated at diagnosis and longitudinally., Results: Anti-Jo1 IgG from serum and BALF bound HisRS-FL, WHEP, and SV with high reactivity at the time of diagnosis and recognized both conformation-dependent and conformation-independent HisRS epitopes. Anti-HisRS-FL IgG displayed high affinity early in the disease. At the time of IIM/ASSD diagnosis, the highest autoantibody levels against HisRS-FL were found in patients ever developing interstitial lung disease (ILD) and arthritis, but with less skin involvement. Moreover, the reactivity of anti-WHEP IgG in BALF correlated with poor pulmonary function. Levels of autoantibodies against HisRS-FL, HisRS domains, and HisRS splice variant generally decreased over time. With some exceptions, longitudinal anti-HisRS-FL antibody levels changed in line with ILD activity., Conclusion: High levels and high-affinity anti-Jo1 autoantibodies towards HisRS-FL were found early in disease in sera and BALF. In combination with the correlation of anti-HisRS-FL antibody levels with ILD and ILD activity in longitudinal samples as well as of anti-WHEP IgG in BALF with poor pulmonary function, this supports the previously raised hypothesis that the lung might have a role in the immune reaction in anti-Jo1-positive patients., (© 2022. The Author(s).)- Published
- 2022
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23. Selection and structural characterization of anti-TREM2 scFvs that reduce levels of shed ectodomain.
- Author
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Szykowska A, Chen Y, Smith TB, Preger C, Yang J, Qian D, Mukhopadhyay SM, Wigren E, Neame SJ, Gräslund S, Persson H, Atkinson PJ, Di Daniel E, Mead E, Wang J, Davis JB, Burgess-Brown NA, and Bullock AN
- Subjects
- HEK293 Cells, Humans, Phagocytosis physiology, Single-Chain Antibodies, Epitopes metabolism, Membrane Glycoproteins metabolism, Receptors, Immunologic metabolism
- Abstract
Mutations in TREM2, a receptor expressed by microglia in the brain, are associated with an increased risk of neurodegeneration, including Alzheimer's disease. Numerous studies support a role for TREM2 in sensing damaging stimuli and triggering signaling cascades necessary for neuroprotection. Despite its significant role, ligands and regulators of TREM2 activation, and the mechanisms governing TREM2-dependent responses and its cleavage from the membrane, remain poorly characterized. Here, we present phage display generated antibody single-chain variable fragments (scFvs) to human TREM2 immunoglobulin-like domain. Co-crystal structures revealed the binding of two scFvs to an epitope on the TREM2 domain distal to the putative ligand-binding site. Enhanced functional activity was observed for oligomeric scFv species, which inhibited the production of soluble TREM2 in a HEK293 cell model. We hope that detailed characterization of their epitopes and properties will facilitate the use of these renewable binders as structural and functional biology tools for TREM2 research., Competing Interests: Declaration of interests Y.C., S.J.N., P.J.A., and J.W. are employees of Eisai Ltd., and Eisai Inc., (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2021
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24. Screening and Production of Recombinant Human Proteins: Protein Production in E. coli.
- Author
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Burgess-Brown NA, Mahajan P, Strain-Damerell C, Fernandez-Cid A, Gileadi O, and Gräslund S
- Subjects
- Humans, Chromatography, Affinity, Chromatography, Gel, Escherichia coli genetics, Escherichia coli metabolism, Recombinant Proteins biosynthesis, Recombinant Proteins genetics, Recombinant Proteins isolation & purification
- Abstract
In Chapter 3 , we described the Structural Genomics Consortium (SGC) process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E. coli system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify which proteins or truncated versions are expressed in a soluble and stable form suitable for structural studies. In addition, we detail the process for scaling up cultures for large-scale protein purification. This level of production is required to obtain sufficient quantities (i.e., milligram amounts) of protein for further characterization and/or structural studies (e.g., crystallization or cryo-EM experiments). Our standard process is purification by immobilized metal affinity chromatography (IMAC) using nickel resin followed by size exclusion chromatography (SEC), with additional procedures arising from the complexity of the protein itself.
- Published
- 2021
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25. Generation and validation of recombinant antibodies to study human aminoacyl-tRNA synthetases.
- Author
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Preger C, Wigren E, Ossipova E, Marks C, Lengqvist J, Hofström C, Andersson O, Jakobsson PJ, Gräslund S, and Persson H
- Subjects
- Humans, Recombinant Proteins chemistry, Recombinant Proteins immunology, Amino Acyl-tRNA Synthetases chemistry, Amino Acyl-tRNA Synthetases immunology, Single-Chain Antibodies chemistry, Single-Chain Antibodies immunology
- Abstract
Aminoacyl-tRNA synthetases (aaRSs) have long been viewed as mere housekeeping proteins and have therefore often been overlooked in drug discovery. However, recent findings have revealed that many aaRSs have noncanonical functions, and several of the aaRSs have been linked to autoimmune diseases, cancer, and neurological disorders. Deciphering these roles has been challenging because of a lack of tools to enable their study. To help solve this problem, we have generated recombinant high-affinity antibodies for a collection of thirteen cytoplasmic and one mitochondrial aaRSs. Selected domains of these proteins were produced recombinantly in Escherichia coli and used as antigens in phage display selections using a synthetic human single-chain fragment variable library. All targets yielded large sets of antibody candidates that were validated through a panel of binding assays against the purified antigen. Furthermore, the top-performing binders were tested in immunoprecipitation followed by MS for their ability to capture the endogenous protein from mammalian cell lysates. For antibodies targeting individual members of the multi-tRNA synthetase complex, we were able to detect all members of the complex, co-immunoprecipitating with the target, in several cell types. The functionality of a subset of binders for each target was also confirmed using immunofluorescence. The sequences of these proteins have been deposited in publicly available databases and repositories. We anticipate that this open source resource, in the form of high-quality recombinant proteins and antibodies, will accelerate and empower future research of the role of aaRSs in health and disease., Competing Interests: Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article., (© 2020 Preger et al.)
- Published
- 2020
- Full Text
- View/download PDF
26. Circulating Levels of Interferon Regulatory Factor-5 Associates With Subgroups of Systemic Lupus Erythematosus Patients.
- Author
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Idborg H, Zandian A, Ossipova E, Wigren E, Preger C, Mobarrez F, Checa A, Sohrabian A, Pucholt P, Sandling JK, Fernandes-Cerqueira C, Rönnelid J, Oke V, Grosso G, Kvarnström M, Larsson A, Wheelock CE, Syvänen AC, Rönnblom L, Kultima K, Persson H, Gräslund S, Gunnarsson I, Nilsson P, Svenungsson E, and Jakobsson PJ
- Subjects
- Adult, Cluster Analysis, Cross-Sectional Studies, Female, Humans, Male, Middle Aged, Phenotype, Proteomics, Proto-Oncogene Proteins c-ets metabolism, Biomarkers blood, Interferon Regulatory Factors blood, Lupus Erythematosus, Systemic immunology, Organic Cation Transporter 2 blood, S100A12 Protein blood
- Abstract
Systemic Lupus Erythematosus (SLE) is a heterogeneous autoimmune disease, which currently lacks specific diagnostic biomarkers. The diversity within the patients obstructs clinical trials but may also reflect differences in underlying pathogenesis. Our objective was to obtain protein profiles to identify potential general biomarkers of SLE and to determine molecular subgroups within SLE for patient stratification. Plasma samples from a cross-sectional study of well-characterized SLE patients ( n = 379) and matched population controls ( n = 316) were analyzed by antibody suspension bead array targeting 281 proteins. To investigate the differences between SLE and controls, Mann-Whitney U -test with Bonferroni correction, generalized linear modeling and receiver operating characteristics (ROC) analysis were performed. K-means clustering was used to identify molecular SLE subgroups. We identified Interferon regulating factor 5 (IRF5), solute carrier family 22 member 2 (SLC22A2) and S100 calcium binding protein A12 (S100A12) as the three proteins with the largest fold change between SLE patients and controls (SLE/Control = 1.4, 1.4, and 1.2 respectively). The lowest p -values comparing SLE patients and controls were obtained for S100A12, Matrix metalloproteinase-1 (MMP1) and SLC22A2 (p
adjusted = 3 × 10-9 , 3 × 10-6 , and 5 × 10-6 respectively). In a set of 15 potential biomarkers differentiating SLE patients and controls, two of the proteins were transcription factors, i.e., IRF5 and SAM pointed domain containing ETS transcription factor (SPDEF). IRF5 was up-regulated while SPDEF was found to be down-regulated in SLE patients. Unsupervised clustering of all investigated proteins identified three molecular subgroups among SLE patients, characterized by (1) high levels of rheumatoid factor-IgM, (2) low IRF5, and (3) high IRF5. IRF5 expressing microparticles were analyzed by flow cytometry in a subset of patients to confirm the presence of IRF5 in plasma and detection of extracellular IRF5 was further confirmed by immunoprecipitation-mass spectrometry (IP-MS). Interestingly IRF5, a known genetic risk factor for SLE, was detected extracellularly and suggested by unsupervised clustering analysis to differentiate between SLE subgroups. Our results imply a set of circulating molecules as markers of possible pathogenic importance in SLE. We believe that these findings could be of relevance for understanding the pathogenesis and diversity of SLE, as well as for selection of patients in clinical trials.- Published
- 2019
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27. Patients with anti-Jo1 antibodies display a characteristic IgG Fc-glycan profile which is further enhanced in anti-Jo1 autoantibodies.
- Author
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Fernandes-Cerqueira C, Renard N, Notarnicola A, Wigren E, Gräslund S, Zubarev RA, Lundberg IE, and Lundström SL
- Subjects
- Adult, Aged, Autoantibodies blood, Autoantibodies metabolism, Autoimmune Diseases diagnosis, Autoimmune Diseases immunology, Autoimmune Diseases metabolism, Autoimmunity, Case-Control Studies, Female, Glycosylation, Humans, Immunoglobulin Fc Fragments metabolism, Immunoglobulin G blood, Immunoglobulin G metabolism, Male, Middle Aged, Polysaccharides metabolism, Autoantibodies immunology, Autoantigens immunology, Immunoglobulin Fc Fragments immunology, Immunoglobulin G immunology
- Abstract
IgG Fc-glycans affect IgG function and are altered in autoimmune diseases and autoantibodies. Anti-histidyl tRNA synthetase autoantibodies (anti-Jo1) are frequent in patients with idiopathic inflammatory myopathies (IIM) and anti-synthetase syndrome (ASS) with associated interstitial lung disease (ILD). Thus, we hypothesized that the total-IgG Fc-glycans from Jo1
+ versus Jo1- patients and anti-Jo1-IgG would show characteristic differences, and that particular Fc-glycan features would be associated with specific clinical manifestations. By proteomics based mass spectrometry we observed a high abundance of agalactosylated IgG1 Fc-glycans in ASS/IIM patients (n = 44) compared to healthy age matched controls (n = 24). Using intra-individual normalization of the main agalactosylated glycan (FA2) of IgG1 vs FA2-IgG2 , ASS/IIM and controls were distinguished with an area under the curve (AUC) of 79 ± 6%. For Jo1+ patients (n = 19) the AUCs went up to 88 ± 6%. Bisected and afucosylated Fc-glycans were significantly lower in Jo1+ compared to Jo1- patients. Anti-Jo1-IgG enriched from eleven patients contained even significantly lower abundances of bisected, afucosylated and galactosylated forms compared to matched total-IgG. ASS and ILD diagnosis, as well as lysozyme and thrombospondin correlated with Jo1+ characteristic Fc-glycan features. These results suggest that the anti-Jo1+ patient Fc-glycan profile contains phenotype specific features which may underlie the pathogenic role of Jo1 autoantibodies.- Published
- 2018
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28. A DNA-Encoded Library of Chemical Compounds Based on Common Scaffolding Structures Reveals the Impact of Ligand Geometry on Protein Recognition.
- Author
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Favalli N, Biendl S, Hartmann M, Piazzi J, Sladojevich F, Gräslund S, Brown PJ, Näreoja K, Schüler H, Scheuermann J, Franzini R, and Neri D
- Subjects
- Humans, Ligands, Molecular Structure, Protein Binding, Small Molecule Libraries chemistry, Antigens, Neoplasm metabolism, Carbonic Anhydrase IX metabolism, DNA chemistry, Serum Albumin, Human metabolism, Small Molecule Libraries metabolism, Tankyrases metabolism
- Abstract
A DNA-encoded chemical library (DECL) with 1.2 million compounds was synthesized by combinatorial reaction of seven central scaffolds with two sets of 343×492 building blocks. Library screening by affinity capture revealed that for some target proteins, the chemical nature of building blocks dominated the selection results, whereas for other proteins, the central scaffold also crucially contributed to ligand affinity. Molecules based on a 3,5-bis(aminomethyl)benzoic acid core structure were found to bind human serum albumin with a K
d value of 6 nm, while compounds with the same substituents on an equidistant but flexible l-lysine scaffold showed 140-fold lower affinity. A 18 nm tankyrase-1 binder featured l-lysine as linking moiety, while molecules based on d-Lysine or (2S,4S)-amino-l-proline showed no detectable binding to the target. This work suggests that central scaffolds which predispose the orientation of chemical building blocks toward the protein target may enhance the screening productivity of encoded libraries., (© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)- Published
- 2018
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29. In Vivo Biotinylation of Antigens in E. coli.
- Author
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Gräslund S, Savitsky P, and Müller-Knapp S
- Subjects
- Animals, Biotin chemistry, Biotinylation, Carbon-Nitrogen Ligases chemistry, Carbon-Nitrogen Ligases genetics, Cloning, Molecular methods, Escherichia coli chemistry, Escherichia coli Proteins chemistry, Escherichia coli Proteins genetics, Gene Expression, Genetic Vectors genetics, Humans, Repressor Proteins chemistry, Repressor Proteins genetics, Streptavidin chemistry, Antigens chemistry, Antigens genetics, Escherichia coli genetics, Immobilized Proteins chemistry, Immobilized Proteins genetics
- Abstract
Site-specific biotinylation of proteins is often the method of choice to enable efficient immobilization of a protein on a surface without interfering with protein folding. The tight interaction of biotin and streptavidin is frequently used to immobilize an antigen during phage display selections of binders. Here we describe a method of in vivo biotinylation of proteins during expression in E. coli, by tagging the protein with the short biotin acceptor peptide sequence, Avi tag, and co-expression of the E. coli biotin ligase (BirA) resulting in precise biotinylation of a specific lysine residue in the tag.
- Published
- 2017
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30. Antibody Validation by Immunoprecipitation Followed by Mass Spectrometry Analysis.
- Author
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Persson H, Preger C, Marcon E, Lengqvist J, and Gräslund S
- Subjects
- Antibody Specificity, HEK293 Cells, Humans, Peptide Library, Single-Chain Antibodies isolation & purification, Single-Chain Antibodies metabolism, Immunoprecipitation methods, Mass Spectrometry methods, Single-Chain Antibodies immunology
- Abstract
We describe a mass spectrometry-based approach for validation of antibody specificity. This method allows validation of antibodies or antibody fragments, against their endogenous targets. It can assess if the antibody is able to bind to its native antigen in cell lysates among thousands of other proteins, DNA, RNA, and other cellular components. In addition, it identifies other proteins the antibody is able to immunoprecipitate allowing for the assessment of antibody specificity and selectivity. This method is easily scalable, adaptable to different cell lines and conditions and has been shown to be reproducible between multiple laboratories.
- Published
- 2017
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- View/download PDF
31. Apollo-NADP(+): a spectrally tunable family of genetically encoded sensors for NADP(+).
- Author
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Cameron WD, Bui CV, Hutchinson A, Loppnau P, Gräslund S, and Rocheleau JV
- Subjects
- Cells, Cultured, Fluorescence Polarization methods, Fluorescence Resonance Energy Transfer, Glucosephosphate Dehydrogenase chemistry, Glucosephosphate Dehydrogenase genetics, Humans, Hydrogen Peroxide metabolism, Image Processing, Computer-Assisted, NADP metabolism, Oxidants metabolism, Oxidative Stress, Protein Conformation, Biosensing Techniques methods, Glucosephosphate Dehydrogenase metabolism, Insulin-Secreting Cells metabolism, NADP chemistry
- Abstract
NADPH-dependent antioxidant pathways have a critical role in scavenging hydrogen peroxide (H2O2) produced by oxidative phosphorylation. Inadequate scavenging results in H2O2 accumulation and can cause disease. To measure NADPH/NADP(+) redox states, we explored genetically encoded sensors based on steady-state fluorescence anisotropy due to FRET (fluorescence resonance energy transfer) between homologous fluorescent proteins (homoFRET); we refer to these sensors as Apollo sensors. We created an Apollo sensor for NADP(+) (Apollo-NADP(+)) that exploits NADP(+)-dependent homodimerization of enzymatically inactive glucose-6-phosphate dehydrogenase (G6PD). This sensor is reversible, responsive to glucose-stimulated metabolism and spectrally tunable for compatibility with many other sensors. We used Apollo-NADP(+) to study beta cells responding to oxidative stress and demonstrated that NADPH is significantly depleted before H2O2 accumulation by imaging a Cerulean-tagged version of Apollo-NADP(+) with the H2O2 sensor HyPer.
- Published
- 2016
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32. Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins.
- Author
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Zhong N, Loppnau P, Seitova A, Ravichandran M, Fenner M, Jain H, Bhattacharya A, Hutchinson A, Paduch M, Lu V, Olszewski M, Kossiakoff AA, Dowdell E, Koide A, Koide S, Huang H, Nadeem V, Sidhu SS, Greenblatt JF, Marcon E, Arrowsmith CH, Edwards AM, and Gräslund S
- Subjects
- Cloning, Molecular, Humans, Peptide Library, Antibody Formation physiology, Antigens immunology, Immunoglobulin Fab Fragments immunology, Recombinant Proteins immunology
- Abstract
We developed and optimized a high-throughput project workflow to generate renewable recombinant antibodies to human proteins involved in epigenetic signalling. Three different strategies to produce phage display compatible protein antigens in bacterial systems were compared, and we found that in vivo biotinylation through the use of an Avi tag was the most productive method. Phage display selections were performed on 265 in vivo biotinylated antigen domains. High-affinity Fabs (<20nM) were obtained for 196. We constructed and optimized a new expression vector to produce in vivo biotinylated Fabs in E. coli. This increased average yields up to 10-fold, with an average yield of 4 mg/L. For 118 antigens, we identified Fabs that could immunoprecipitate their full-length endogenous targets from mammalian cell lysates. One Fab for each antigen was converted to a recombinant IgG and produced in mammalian cells, with an average yield of 15 mg/L. In summary, we have optimized each step of the pipeline to produce recombinant antibodies, significantly increasing both efficiency and yield, and also showed that these Fabs and IgGs can be generally useful for chromatin immunoprecipitation (ChIP) protocols.
- Published
- 2015
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33. A High Through-put Platform for Recombinant Antibodies to Folded Proteins.
- Author
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Hornsby M, Paduch M, Miersch S, Sääf A, Matsuguchi T, Lee B, Wypisniak K, Doak A, King D, Usatyuk S, Perry K, Lu V, Thomas W, Luke J, Goodman J, Hoey RJ, Lai D, Griffin C, Li Z, Vizeacoumar FJ, Dong D, Campbell E, Anderson S, Zhong N, Gräslund S, Koide S, Moffat J, Sidhu S, Kossiakoff A, and Wells J
- Subjects
- Antigens genetics, Antigens immunology, Escherichia coli genetics, High-Throughput Screening Assays, Protein Folding, RNA, Small Interfering genetics, Recombinant Proteins genetics, Recombinant Proteins immunology, Antibodies genetics, Antibodies immunology, Immunoglobulin Fab Fragments genetics, Immunoglobulin Fab Fragments immunology, Transcription Factors genetics, Transcription Factors immunology
- Abstract
Antibodies are key reagents in biology and medicine, but commercial sources are rarely recombinant and thus do not provide a permanent and renewable resource. Here, we describe an industrialized platform to generate antigens and validated recombinant antibodies for 346 transcription factors (TFs) and 211 epigenetic antigens. We describe an optimized automated phage display and antigen expression pipeline that in aggregate produced about 3000 sequenced Fragment antigen-binding domain that had high affinity (typically EC50<20 nm), high stability (Tm∼80 °C), good expression in E. coli (∼5 mg/L), and ability to bind antigen in complex cell lysates. We evaluated a subset of Fabs generated to homologous SCAN domains for binding specificities. These Fragment antigen-binding domains were monospecific to their target SCAN antigen except in rare cases where they cross-reacted with a few highly related antigens. Remarkably, immunofluorescence experiments in six cell lines for 270 of the TF antigens, each having multiple antibodies, show that ∼70% stain predominantly in the cytosol and ∼20% stain in the nucleus which reinforces the dominant role that translocation plays in TF biology. These cloned antibody reagents are being made available to the academic community through our web site recombinant-antibodies.org to allow a more system-wide analysis of TF and chromatin biology. We believe these platforms, infrastructure, and automated approaches will facilitate the next generation of renewable antibody reagents to the human proteome in the coming decade., (© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.)
- Published
- 2015
- Full Text
- View/download PDF
34. Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step.
- Author
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Huang R, Gorman KT, Vinci CR, Dobrovetsky E, Gräslund S, and Kay BK
- Subjects
- Amino Acid Motifs, Amino Acid Sequence, Antibodies metabolism, Antigens metabolism, Biotinylation, Calorimetry, Cell Surface Display Techniques, HeLa Cells, Humans, Indicators and Reagents, Kinetics, Molecular Sequence Data, Protein Structure, Secondary, Chromatography, Affinity methods, Recombinant Proteins metabolism
- Abstract
Often when generating recombinant affinity reagents to a target, one singles out an individual binder, constructs a secondary library of variants, and affinity selects a tighter or more specific binder. To enhance the throughput of this general approach, we have developed a more integrated strategy where the "affinity maturation" step is part of the phage-display pipeline, rather than a follow-on process. In our new schema, we perform two rounds of affinity selection, followed by error-prone PCR on the pools of recovered clones, generation of secondary libraries, and three additional rounds of affinity selection, under conditions of off-rate competition. We demonstrate the utility of this approach by generating low nanomolar fibronectin type III (FN3) monobodies to five human proteins: ubiquitin-conjugating enzyme E2 R1 (CDC34), COP9 signalosome complex subunit 5 (COPS5), mitogen-activated protein kinase kinase 5 (MAP2K5), Splicing factor 3A subunit 1 (SF3A1) and ubiquitin carboxyl-terminal hydrolase 11 (USP11). The affinities of the resulting monobodies are typically in the single-digit nanomolar range. We demonstrate the utility of two binders by pulling down the targets from a spiked lysate of HeLa cells. This integrated approach should be applicable to directed evolution of any phage-displayed affinity reagent scaffold.
- Published
- 2015
- Full Text
- View/download PDF
35. Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
- Author
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Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, Byram G, Collins BC, Dowdell E, Fenner M, Guo X, Hutchinson A, Kennedy JJ, Krastins B, Larsen B, Lin ZY, Lopez MF, Loppnau P, Miersch S, Nguyen T, Olsen JB, Paduch M, Ravichandran M, Seitova A, Vadali G, Vogelsang MS, Whiteaker JR, Zhong G, Zhong N, Zhao L, Aebersold R, Arrowsmith CH, Emili A, Frappier L, Gingras AC, Gstaiger M, Paulovich AG, Koide S, Kossiakoff AA, Sidhu SS, Wodak SJ, Gräslund S, Greenblatt JF, and Edwards AM
- Subjects
- Cloning, Molecular, Computational Biology methods, Escherichia coli metabolism, HEK293 Cells, Humans, Immunoglobulin Fragments chemistry, Immunoglobulin G chemistry, Mass Spectrometry methods, Peptide Library, Proteins chemistry, Proteome, Reproducibility of Results, Antibodies, Monoclonal chemistry, Antibody Specificity, Chromatin chemistry, Immunoprecipitation methods, Proteomics methods
- Abstract
Antibodies are used in multiple cell biology applications, but there are no standardized methods to assess antibody quality-an absence that risks data integrity and reproducibility. We describe a mass spectrometry-based standard operating procedure for scoring immunoprecipitation antibody quality. We quantified the abundance of all the proteins in immunoprecipitates of 1,124 new recombinant antibodies for 152 chromatin-related human proteins by comparing normalized spectral abundance factors from the target antigen with those of all other proteins. We validated the performance of the standard operating procedure in blinded studies in five independent laboratories. Antibodies for which the target antigen or a member of its known protein complex was the most abundant protein were classified as 'IP gold standard'. This method generates quantitative outputs that can be stored and archived in public databases, and it represents a step toward a platform for community benchmarking of antibody quality.
- Published
- 2015
- Full Text
- View/download PDF
36. Structural Basis for the Specificity of Human NUDT16 and Its Regulation by Inosine Monophosphate.
- Author
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Trésaugues L, Lundbäck T, Welin M, Flodin S, Nyman T, Silvander C, Gräslund S, and Nordlund P
- Subjects
- Adenosine Diphosphate metabolism, Biocatalysis drug effects, Calorimetry, Conserved Sequence, Crystallography, X-Ray, Humans, Inosine Monophosphate pharmacology, Kinetics, Protein Binding drug effects, Protein Multimerization drug effects, Structure-Activity Relationship, Substrate Specificity drug effects, Inosine Monophosphate metabolism, Pyrophosphatases chemistry, Pyrophosphatases metabolism
- Abstract
Human NUDT16 is a member of the NUDIX hydrolase superfamily. After having been initially described as an mRNA decapping enzyme, recent studies conferred it a role as an "housecleaning" enzyme specialized in the removal of hazardous (deoxy)inosine diphosphate from the nucleotide pool. Here we present the crystal structure of human NUDT16 both in its apo-form and in complex with its product inosine monophosphate (IMP). NUDT16 appears as a dimer whose formation generates a positively charged trench to accommodate substrate-binding. Complementation of the structural data with detailed enzymatic and biophysical studies revealed the determinants of substrate recognition and particularly the importance of the substituents in position 2 and 6 on the purine ring. The affinity for the IMP product, harboring a carbonyl in position 6 on the base, compared to purine monophosphates lacking a H-bond acceptor in this position, implies a catalytic cycle whose rate is primarily regulated by the product-release step. Finally, we have also characterized a phenomenon of inhibition by the product of the reaction, IMP, which might exclude non-deleterious nucleotides from NUDT16-mediated hydrolysis regardless of their cellular concentration. Taken together, this study details structural and regulatory mechanisms explaining how substrates are selected for hydrolysis by human NUDT16.
- Published
- 2015
- Full Text
- View/download PDF
37. Tankyrase 1 Inhibitors with Drug-like Properties Identified by Screening a DNA-Encoded Chemical Library.
- Author
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Samain F, Ekblad T, Mikutis G, Zhong N, Zimmermann M, Nauer A, Bajic D, Decurtins W, Scheuermann J, Brown PJ, Hall J, Gräslund S, Schüler H, Neri D, and Franzini RM
- Subjects
- Amines chemistry, Amines pharmacology, Carboxylic Acids chemistry, Carboxylic Acids pharmacology, Gene Library, Humans, Models, Molecular, Tankyrases metabolism, Enzyme Inhibitors chemistry, Enzyme Inhibitors pharmacology, Small Molecule Libraries chemistry, Small Molecule Libraries pharmacology, Tankyrases antagonists & inhibitors
- Abstract
We describe the synthesis and screening of a DNA-encoded chemical library containing 76230 compounds. In this library, sets of amines and carboxylic acids are directly linked producing encoded compounds with compact structures and drug-like properties. Affinity screening of this library yielded inhibitors of the potential pharmaceutical target tankyrase 1, a poly(ADP-ribose) polymerase. These compounds have drug-like characteristics, and the most potent hit compound (X066/Y469) inhibited tankyrase 1 with an IC50 value of 250 nM.
- Published
- 2015
- Full Text
- View/download PDF
38. Identification of structure-activity relationships from screening a structurally compact DNA-encoded chemical library.
- Author
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Franzini RM, Ekblad T, Zhong N, Wichert M, Decurtins W, Nauer A, Zimmermann M, Samain F, Scheuermann J, Brown PJ, Hall J, Gräslund S, Schüler H, and Neri D
- Subjects
- DNA Fingerprinting, DNA Probes chemistry, Enzyme Inhibitors chemical synthesis, Enzyme Inhibitors pharmacology, High-Throughput Screening Assays, Humans, Ligands, Prostate-Specific Antigen drug effects, Serum Albumin chemistry, Small Molecule Libraries, Structure-Activity Relationship, Tankyrases antagonists & inhibitors, DNA chemistry, DNA Probes chemical synthesis
- Abstract
Methods for the rapid and inexpensive discovery of hit compounds are essential for pharmaceutical research and DNA-encoded chemical libraries represent promising tools for this purpose. We here report on the design and synthesis of DAL-100K, a DNA-encoded chemical library containing 103 200 structurally compact compounds. Affinity screening experiments and DNA-sequencing analysis provided ligands with nanomolar affinities to several proteins, including prostate-specific membrane antigen and tankyrase 1. Correlations of sequence counts with binding affinities and potencies of enzyme inhibition were observed and enabled the identification of structural features critical for activity. These results indicate that libraries of this type represent a useful source of small-molecule binders for target proteins of pharmaceutical interest and information on structural features important for binding., (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2015
- Full Text
- View/download PDF
39. Lysine demethylase KDM4A associates with translation machinery and regulates protein synthesis.
- Author
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Van Rechem C, Black JC, Boukhali M, Aryee MJ, Gräslund S, Haas W, Benes CH, and Whetstine JR
- Subjects
- Aminopyridines pharmacology, Drug Resistance genetics, Humans, Hydrazones pharmacology, Jumonji Domain-Containing Histone Demethylases antagonists & inhibitors, Methylation, Peptide Chain Initiation, Translational, Peptide Initiation Factors metabolism, Protein Binding, Protein Kinase Inhibitors pharmacology, Ribosomal Proteins metabolism, TOR Serine-Threonine Kinases antagonists & inhibitors, Jumonji Domain-Containing Histone Demethylases genetics, Jumonji Domain-Containing Histone Demethylases metabolism, Lysine metabolism, Protein Biosynthesis drug effects
- Abstract
Unlabelled: Chromatin-modifying enzymes are predominantly nuclear; however, these factors are also localized to the cytoplasm, and very little is known about their role in this compartment. In this report, we reveal a non-chromatin-linked role for the lysine-specific demethylase KDM4A. We demonstrate that KDM4A interacts with the translation initiation complex and affects the distribution of translation initiation factors within polysome fractions. Furthermore, KDM4A depletion reduced protein synthesis and enhanced the protein synthesis suppression observed with mTOR inhibitors, which paralleled an increased sensitivity to these drugs. Finally, we demonstrate that JIB-04, a JmjC demethylase inhibitor, suppresses translation initiation and enhances mTOR inhibitor sensitivity. These data highlight an unexpected cytoplasmic role for KDM4A in regulating protein synthesis and suggest novel potential therapeutic applications for this class of enzyme., Significance: This report documents an unexpected cytoplasmic role for the lysine demethylase KDM4A. We demonstrate that KDM4A interacts with the translation initiation machinery, regulates protein synthesis and, upon coinhibition with mTOR inhibitors, enhances the translation suppression and cell sensitivity to these therapeutics., (©2015 American Association for Cancer Research.)
- Published
- 2015
- Full Text
- View/download PDF
40. Recombinant renewable polyclonal antibodies.
- Author
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Ferrara F, D'Angelo S, Gaiotto T, Naranjo L, Tian H, Gräslund S, Dobrovetsky E, Hraber P, Lund-Johansen F, Saragozza S, Sblattero D, Kiss C, and Bradbury AR
- Subjects
- Epitopes chemistry, Epitopes genetics, Humans, Recombinant Proteins chemistry, Recombinant Proteins genetics, Antibodies chemistry, Antibodies genetics, Gene Library
- Abstract
Only a small fraction of the antibodies in a traditional polyclonal antibody mixture recognize the target of interest, frequently resulting in undesirable polyreactivity. Here, we show that high-quality recombinant polyclonals, in which hundreds of different antibodies are all directed toward a target of interest, can be easily generated in vitro by combining phage and yeast display. We show that, unlike traditional polyclonals, which are limited resources, recombinant polyclonal antibodies can be amplified over one hundred million-fold without losing representation or functionality. Our protocol was tested on 9 different targets to demonstrate how the strategy allows the selective amplification of antibodies directed toward desirable target specific epitopes, such as those found in one protein but not a closely related one, and the elimination of antibodies recognizing common epitopes, without significant loss of diversity. These recombinant renewable polyclonal antibodies are usable in different assays, and can be generated in high throughput. This approach could potentially be used to develop highly specific recombinant renewable antibodies against all human gene products.
- Published
- 2015
- Full Text
- View/download PDF
41. Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
- Author
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Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Gräslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, and Greenblatt J
- Subjects
- Carrier Proteins genetics, HEK293 Cells, Humans, Membrane Proteins genetics, Protein Binding, Carrier Proteins metabolism, Chromatin metabolism, Chromosome Segregation, Histone Demethylases metabolism, Membrane Proteins metabolism
- Abstract
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate a protein-protein interaction map encompassing known and predicted chromatin-related proteins. On the basis of 1,394 successful purifications of 293 proteins, we report a high-confidence (85% precision) network involving 11,464 protein-protein interactions among 1,738 different human proteins, grouped into 164 often overlapping protein complexes with a particular focus on the family of JmjC-containing lysine demethylases, their partners, and their roles in chromatin remodeling. We show that RCCD1 is a partner of histone H3K36 demethylase KDM8 and demonstrate that both are important for cell-cycle-regulated transcriptional repression in centromeric regions and accurate mitotic division., (Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
42. A Basic Post-SET Extension of NSDs Is Essential for Nucleosome Binding In Vitro.
- Author
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Allali-Hassani A, Kuznetsova E, Hajian T, Wu H, Dombrovski L, Li Y, Gräslund S, Arrowsmith CH, Schapira M, and Vedadi M
- Subjects
- Buffers, Cloning, Molecular, Gene Deletion, Histone Methyltransferases, Histones chemistry, Humans, Inhibitory Concentration 50, Kinetics, Lysine chemistry, Models, Molecular, Mutation, Protein Binding, Substrate Specificity, Histone-Lysine N-Methyltransferase chemistry, Intracellular Signaling Peptides and Proteins chemistry, Nuclear Proteins chemistry, Nucleosomes chemistry, Repressor Proteins chemistry
- Abstract
The nuclear receptor SET domain-containing family of proteins (NSD1, NSD2, and NSD3) is known to mono- and dimethylate lysine 36 of histone H3 (H3K36). Overexpression and translocation of NSDs have been widely implicated in a variety of diseases including cancers. Although the substrate specificity of NSDs has been a subject of many valuable studies, the activity of these proteins has never been fully characterized in vitro. In this study, we present full kinetic characterization of NSD1, NSD2, and NSD3 and provide robust in vitro assays suitable for screening these proteins in a 384-well format using nucleosome as a substrate. Through monitoring the changes in substrate specificity of a series of NSD constructs and using molecular modeling, we show that a basic post-SET extension common to all three NSDs (corresponding to residues 1209 to 1226 of NSD2) is essential for proper positioning on nucleosome substrates., (© 2014 Society for Laboratory Automation and Screening.)
- Published
- 2014
- Full Text
- View/download PDF
43. Structural basis for phosphoinositide substrate recognition, catalysis, and membrane interactions in human inositol polyphosphate 5-phosphatases.
- Author
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Trésaugues L, Silvander C, Flodin S, Welin M, Nyman T, Gräslund S, Hammarström M, Berglund H, and Nordlund P
- Subjects
- Catalytic Domain, Crystallography, X-Ray, Humans, Inositol Phosphates chemistry, Inositol Phosphates metabolism, Models, Molecular, Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases, Phosphatidylinositols chemistry, Phosphatidylinositols metabolism, Substrate Specificity, Cell Membrane metabolism, Phosphoric Monoester Hydrolases chemistry, Phosphoric Monoester Hydrolases metabolism
- Abstract
SHIP2, OCRL, and INPP5B belong to inositol polyphosphate 5-phophatase subfamilies involved in insulin regulation and Lowes syndrome. The structural basis for membrane recognition, substrate specificity, and regulation of inositol polyphosphate 5-phophatases is still poorly understood. We determined the crystal structures of human SHIP2, OCRL, and INPP5B, the latter in complex with phosphoinositide substrate analogs, which revealed a membrane interaction patch likely to assist in sequestering substrates from the lipid bilayer. Residues recognizing the 1-phosphate of the substrates are highly conserved among human family members, suggesting similar substrate binding modes. However, 3- and 4-phosphate recognition varies and determines individual substrate specificity profiles. The high conservation of the environment of the scissile 5-phosphate suggests a common reaction geometry for all members of the human 5-phosphatase family., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
44. Medium-throughput production of recombinant human proteins: protein production in E. coli.
- Author
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Burgess-Brown NA, Mahajan P, Strain-Damerell C, Gileadi O, and Gräslund S
- Subjects
- Batch Cell Culture Techniques, Escherichia coli genetics, Escherichia coli metabolism, Genetic Vectors genetics, Humans, Quality Control, Recombinant Fusion Proteins isolation & purification, Transformation, Bacterial, Cloning, Molecular methods, Proteins genetics, Proteins metabolism, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins genetics
- Abstract
In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E. coli system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify which proteins or truncated versions are expressed in a soluble and stable form suitable for structural studies. In addition, we detail the process for scaling up cultures for large-scale protein purification. This level of production is required to obtain sufficient quantities (i.e., milligram amounts) of protein for further characterization and/or crystallization experiments. Our standard process is purification by immobilized metal affinity chromatography (IMAC) using nickel resin followed by size exclusion chromatography (SEC), with additional procedures arising from the complexity of the protein itself.
- Published
- 2014
- Full Text
- View/download PDF
45. Substrate specificity and oligomerization of human GMP synthetase.
- Author
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Welin M, Lehtiö L, Johansson A, Flodin S, Nyman T, Trésaugues L, Hammarström M, Gräslund S, and Nordlund P
- Subjects
- Amino Acid Sequence, Escherichia coli chemistry, Escherichia coli enzymology, Humans, Kinetics, Models, Molecular, Molecular Sequence Data, Protein Binding, Protein Conformation, Protein Interaction Domains and Motifs, Sequence Alignment, Substrate Specificity, Carbon-Nitrogen Ligases chemistry, Carbon-Nitrogen Ligases metabolism, Protein Multimerization
- Abstract
Guanine monophosphate (GMP) synthetase is a bifunctional two-domain enzyme. The N-terminal glutaminase domain generates ammonia from glutamine and the C-terminal synthetase domain aminates xanthine monophosphate (XMP) to form GMP. Mammalian GMP synthetases (GMPSs) contain a 130-residue-long insert in the synthetase domain in comparison to bacterial proteins. We report here the structure of a eukaryotic GMPS. Substrate XMP was bound in the crystal structure of the human GMPS enzyme. XMP is bound to the synthetase domain and covered by a LID motif. The enzyme forms a dimer in the crystal structure with subunit orientations entirely different from the bacterial counterparts. The inserted sub-domain is shown to be involved in substrate binding and dimerization. Furthermore, the structural basis for XMP recognition is revealed as well as a potential allosteric site. Enzymes in the nucleotide metabolism typically display an increased activity in proliferating cells due to the increased need for nucleotides. Many drugs used as immunosuppressants and for treatment of cancer and viral diseases are indeed nucleobase- and nucleoside-based compounds, which are acting on or are activated by enzymes in this pathway. The information obtained from the crystal structure of human GMPS might therefore aid in understanding interactions of nucleoside-based drugs with GMPS and in structure-based design of GMPS-specific inhibitors., (© 2013 The Authors. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
46. Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation.
- Author
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Keates T, Cooper CD, Savitsky P, Allerston CK, Phillips C, Hammarström M, Daga N, Berridge G, Mahajan P, Burgess-Brown NA, Müller S, Gräslund S, and Gileadi O
- Subjects
- Animals, Biotin metabolism, Biotinylation, Crystallization, Culture Media, Genes, Humans, Mass Spectrometry, Plasmids metabolism, Protein Structure, Tertiary, Proteome genetics, Proteome isolation & purification, Solubility, Chromatography, Affinity methods, Proteome chemistry, Proteome metabolism, Proteomics methods
- Abstract
The generation of affinity reagents to large numbers of human proteins depends on the ability to express the target proteins as high-quality antigens. The Structural Genomics Consortium (SGC) focuses on the production and structure determination of human proteins. In a 7-year period, the SGC has deposited crystal structures of >800 human protein domains, and has additionally expressed and purified a similar number of protein domains that have not yet been crystallised. The targets include a diversity of protein domains, with an attempt to provide high coverage of protein families. The family approach provides an excellent basis for characterising the selectivity of affinity reagents. We present a summary of the approaches used to generate purified human proteins or protein domains, a test case demonstrating the ability to rapidly generate new proteins, and an optimisation study on the modification of >70 proteins by biotinylation in vivo. These results provide a unique synergy between large-scale structural projects and the recent efforts to produce a wide coverage of affinity reagents to the human proteome., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
47. Pan-pathway based interaction profiling of FDA-approved nucleoside and nucleobase analogs with enzymes of the human nucleotide metabolism.
- Author
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Egeblad L, Welin M, Flodin S, Gräslund S, Wang L, Balzarini J, Eriksson S, and Nordlund P
- Subjects
- Drug Design, Enzyme Assays methods, Enzymes metabolism, Guanine Deaminase chemistry, Guanine Deaminase metabolism, Humans, Kinetics, Metabolic Networks and Pathways, Nucleosides chemistry, Nucleotides chemistry, Ribonucleotide Reductases chemistry, Ribonucleotide Reductases metabolism, Uridine Phosphorylase metabolism, Nucleosides metabolism, Nucleotides metabolism
- Abstract
To identify interactions a nucleoside analog library (NAL) consisting of 45 FDA-approved nucleoside analogs was screened against 23 enzymes of the human nucleotide metabolism using a thermal shift assay. The method was validated with deoxycytidine kinase; eight interactions known from the literature were detected and five additional interactions were revealed after the addition of ATP, the second substrate. The NAL screening gave relatively few significant hits, supporting a low rate of "off target effects." However, unexpected ligands were identified for two catabolic enzymes guanine deaminase (GDA) and uridine phosphorylase 1 (UPP1). An acyclic guanosine prodrug analog, valaciclovir, was shown to stabilize GDA to the same degree as the natural substrate, guanine, with a ΔT(agg) around 7°C. Aciclovir, penciclovir, ganciclovir, thioguanine and mercaptopurine were also identified as ligands for GDA. The crystal structure of GDA with valaciclovir bound in the active site was determined, revealing the binding of the long unbranched chain of valaciclovir in the active site of the enzyme. Several ligands were identified for UPP1: vidarabine, an antiviral nucleoside analog, as well as trifluridine, idoxuridine, floxuridine, zidovudine, telbivudine, fluorouracil and thioguanine caused concentration-dependent stabilization of UPP1. A kinetic study of UPP1 with vidarabine revealed that vidarabine was a mixed-type competitive inhibitor with the natural substrate uridine. The unexpected ligands identified for UPP1 and GDA imply further metabolic consequences for these nucleoside analogs, which could also serve as a starting point for future drug design.
- Published
- 2012
- Full Text
- View/download PDF
48. Recombinant protein quality evaluation: proposal for a minimal information standard.
- Author
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Buckle AM, Bate MA, Androulakis S, Cinquanta M, Basquin J, Bonneau F, Chatterjee DK, Cittaro D, Gräslund S, Gruszka A, Page R, Suppmann S, Wheeler JX, Agostini D, Taussig M, Taylor CF, Bottomley SP, Villaverde A, and de Marco A
- Published
- 2011
- Full Text
- View/download PDF
49. A roadmap to generate renewable protein binders to the human proteome.
- Author
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Colwill K and Gräslund S
- Subjects
- Animals, Antibodies, Monoclonal immunology, Cell Line, Dogs, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Humans, Immunoblotting, Immunoprecipitation, Multicenter Studies as Topic, Protein Binding, Proteome chemistry, Recombinant Proteins chemistry, Surface Plasmon Resonance, src Homology Domains immunology, Protein Array Analysis, Proteins chemistry, Proteins immunology, Proteome immunology
- Abstract
Despite the wealth of commercially available antibodies to human proteins, research is often hindered by their inconsistent validation, their poor performance and the inadequate coverage of the proteome. These issues could be addressed by systematic, genome-wide efforts to generate and validate renewable protein binders. We report a multicenter study to assess the potential of hybridoma and phage-display technologies in a coordinated large-scale antibody generation and validation effort. We produced over 1,000 antibodies targeting 20 SH2 domain proteins and evaluated them for potency and specificity by enzyme-linked immunosorbent assay (ELISA), protein microarray and surface plasmon resonance (SPR). We also tested selected antibodies in immunoprecipitation, immunoblotting and immunofluorescence assays. Our results show that high-affinity, high-specificity renewable antibodies generated by different technologies can be produced quickly and efficiently. We believe that this work serves as a foundation and template for future larger-scale studies to create renewable protein binders.
- Published
- 2011
- Full Text
- View/download PDF
50. A secretory system for bacterial production of high-profile protein targets.
- Author
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Kotzsch A, Vernet E, Hammarström M, Berthelsen J, Weigelt J, Gräslund S, and Sundström M
- Subjects
- Bacterial Outer Membrane Proteins genetics, Bacterial Outer Membrane Proteins metabolism, Escherichia coli genetics, Escherichia coli metabolism, Escherichia coli Proteins genetics, Humans, Mass Spectrometry methods, Periplasmic Binding Proteins genetics, Periplasmic Binding Proteins metabolism, Porins genetics, Porins metabolism, Promoter Regions, Genetic, Recombinant Fusion Proteins genetics, Bacterial Secretion Systems physiology, Escherichia coli Proteins metabolism, Recombinant Fusion Proteins biosynthesis
- Abstract
Escherichia coli represents a robust, inexpensive expression host for the production of recombinant proteins. However, one major limitation is that certain protein classes do not express well in a biologically relevant form using standard expression approaches in the cytoplasm of E. coli. To improve the usefulness of the E. coli expression platform we have investigated combinations of promoters and selected N-terminal fusion tags for the extracellular expression of human target proteins. A comparative study was conducted on 24 target proteins fused to outer membrane protein A (OmpA), outer membrane protein F (OmpF) and osmotically inducible protein Y (OsmY). Based on the results of this initial study, we carried out an extended expression screen employing the OsmY fusion and multiple constructs of a more diverse set of human proteins. Using this high-throughput compatible system, we clearly demonstrate that secreted biomedically relevant human proteins can be efficiently retrieved and purified from the growth medium., (Copyright © 2011 The Protein Society.)
- Published
- 2011
- Full Text
- View/download PDF
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