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3. Penile fibropapilloma in a Belgian Blue breeding bull.

Author

Beci, B., Proost, K., Govaere, J., Opsomer, G., and Meesters, M.

Subjects
PUDENDAL nerve, NERVE block, WARTS, PENIS, BULLS
Abstract

Copyright of Vlaams Diergeneeskundig Tijdschrift is the property of Ghent University, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2024
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4. Development of the first equine blastocyst produced by conventional IVF and in vitro culture in Europe resulting in the birth of a foal.

Author

Papas, M., Govaere, J., Peere, S., Gerits, I., Van den Branden, E., Fernández-Montoro, A., De Coster, T., Hedia, M., Angel-Velez, D., Van Soom, A., and Smits, K.

Subjects
FOALS, FERTILIZATION in vitro, EMBRYO transfer, BLASTOCYST, OVUM, MANUFACTURING processes, OVARIES
Abstract

Copyright of Vlaams Diergeneeskundig Tijdschrift is the property of Ghent University, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2024
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15. An improved vitrification protocol for equine immature oocytes, resulting in a first live foal

Author

Ortiz-Escribano, N., Bogado Pascottini, O., Woelders, H., Vandenberghe, L., De Schauwer, C., Govaere, J., Van den Abbeel, E., Vullers, T., Ververs, C., Roels, K., Van De Velde, M., van Soom, A., Smits, K., Ortiz-Escribano, N., Bogado Pascottini, O., Woelders, H., Vandenberghe, L., De Schauwer, C., Govaere, J., Van den Abbeel, E., Vullers, T., Ververs, C., Roels, K., Van De Velde, M., van Soom, A., and Smits, K.

Abstract

Background: The success rate for vitrification of immature equine oocytes is low. Although vitrified-warmed oocytes are able to mature, further embryonic development appears to be compromised. Objectives: The aim of this study was to compare two vitrification protocols, and to examine the effect of the number of layers of cumulus cells surrounding the oocyte during vitrification of immature equine oocytes. Study design: Experimental in vitro and in vivo trials. Methods: Immature equine oocytes were vitrified after a short exposure to high concentrations of cryoprotective agents (CPAs), or a long exposure to lower concentrations of CPAs. In Experiment 1, the maturation of oocytes surrounded by multiple layers of cumulus cells (CC oocytes) and oocytes surrounded by only corona radiata (CR oocytes) was investigated. In Experiment 2, spindle configuration was determined for CR oocytes vitrified using the two vitrification protocols. In Experiment 3, further embryonic development was studied after fertilisation and culture. Embryo transfer was performed in a standard manner. Results: Similar nuclear maturation rates were observed for CR oocytes vitrified using the long exposure and nonvitrified controls. Furthermore, a lower maturation rate was obtained for CC oocytes vitrified with the short exposure compared to control CR oocytes (P = 0.001). Both vitrification protocols resulted in significantly higher rates of aberrant spindle configuration than the control groups (P<0.05). Blastocyst development only occurred in CR oocytes vitrified using the short vitrification protocol, and even though blastocyst rates were significantly lower than in the control group (P<0.001), transfer of five embryos resulted in one healthy foal. Main limitations: The relatively low number of equine oocytes and embryo transfer procedures performed. Conclusions: For vitrification of immature equine oocytes, the use of 1) CR oocytes, 2) a high concentration of CPAs, and 3) a short exposure time

Published
2018

18. Ascending placentitis in the mare

Author

Govaere, J., primary, Roels, K., additional, Ververs, C., additional, Van de Velde, M., additional, De Lange, V., additional, Gerits, I., additional, Hoogewijs, M., additional, and Van Soom, A., additional

Published
2018
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21. An improved vitrification protocol for equine immature oocytes, resulting in a first live foal

Author

Ortiz-Escribano, N., primary, Bogado Pascottini, O., additional, Woelders, H., additional, Vandenberghe, L., additional, De Schauwer, C., additional, Govaere, J., additional, Van den Abbeel, E., additional, Vullers, T., additional, Ververs, C., additional, Roels, K., additional, Van De Velde, M., additional, Van Soom, A., additional, and Smits, K., additional

Published
2017
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24. Limitations of a chromogenic agar plate for the identifying bacteria isolated from equine endometritis samples.

Author

Vera, L., Boyen, F., Visscher, A., Vandenbroucke, V., Vanantwerpen, G., and Govaere, J.

Abstract

Summary: Background: The use of commercial chromogenic agar plates for the rapid, easy and correct identification of equine endometritis‐causing bacteria has been proposed. Preliminary tests in our lab revealed undescribed limitations. Therefore, we tested the ability of the Brilliance UTI agar, a commercially available chromogenic agar, to accurately identify bacteria causing equine endometritis. Objectives: To 1) investigate whether bacteria present in the equine uterus are able to grow on this chromogenic agar plate, 2) determine whether these bacteria belong to the genera for which these agar plates were originally designed and 3) consider whether these bacterial genera can be correctly identified, based only on the colour appearance. Study design: In vitro experiments. Methods: Macroscopic growth and colour appearance on the Brilliance UTI agar of 58 bacterial isolates, from previously collected equine uterine samples, were evaluated. Isolates were tentatively identified at the genus level using the manufacturer's guidelines and results were compared with MALDI‐TOF MS as a "gold standard". Results: The study revealed that 1) 77% (N = 45/58) of the bacterial isolates grew well on this chromogenic agar, 2) 83% of the investigated isolates (N = 48/58) belonged to one of the genera for which guidelines for identification were provided by the manufacturer and 3) only 50% of the isolates (N = 29/58) were correctly identified at the genus level, based only on colour appearance. Main limitations: The current study uses purified bacterial isolates to inoculate the chromogenic agar plates, instead of fresh uterine samples. Bacteria were identified to the genus level using MALDI‐TOF MS. Conclusion: This study shows that identification at the genus level based only on colour appearance, without additional tests or the simultaneous use of other media, is not reliable based on the existing identification guidelines. [ABSTRACT FROM AUTHOR]

Published
2019
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27. An improved vitrification protocol for equine immature oocytes, resulting in a first live foal.

Author

Ortiz‐Escribano, N., Bogado Pascottini, O., Woelders, H., Vandenberghe, L., De Schauwer, C., Govaere, J., Van den Abbeel, E., Vullers, T., Ververs, C., Roels, K., Van De Velde, M., Van Soom, A., and Smits, K.

Abstract

Summary: Background: The success rate for vitrification of immature equine oocytes is low. Although vitrified‐warmed oocytes are able to mature, further embryonic development appears to be compromised. Objectives: The aim of this study was to compare two vitrification protocols, and to examine the effect of the number of layers of cumulus cells surrounding the oocyte during vitrification of immature equine oocytes. Study design: Experimental in vitro and in vivo trials. Methods: Immature equine oocytes were vitrified after a short exposure to high concentrations of cryoprotective agents (CPAs), or a long exposure to lower concentrations of CPAs. In Experiment 1, the maturation of oocytes surrounded by multiple layers of cumulus cells (CC oocytes) and oocytes surrounded by only corona radiata (CR oocytes) was investigated. In Experiment 2, spindle configuration was determined for CR oocytes vitrified using the two vitrification protocols. In Experiment 3, further embryonic development was studied after fertilisation and culture. Embryo transfer was performed in a standard manner. Results: Similar nuclear maturation rates were observed for CR oocytes vitrified using the long exposure and nonvitrified controls. Furthermore, a lower maturation rate was obtained for CC oocytes vitrified with the short exposure compared to control CR oocytes (P = 0.001). Both vitrification protocols resulted in significantly higher rates of aberrant spindle configuration than the control groups (P<0.05). Blastocyst development only occurred in CR oocytes vitrified using the short vitrification protocol, and even though blastocyst rates were significantly lower than in the control group (P<0.001), transfer of five embryos resulted in one healthy foal. Main limitations: The relatively low number of equine oocytes and embryo transfer procedures performed. Conclusions: For vitrification of immature equine oocytes, the use of 1) CR oocytes, 2) a high concentration of CPAs, and 3) a short exposure time may be key factors for maintaining developmental competence. [ABSTRACT FROM AUTHOR]

Published
2018
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28. Preputial fibroma in a gelding

Author

De Meyer, A., primary, Vandenabeele, S., additional, Ververs, C., additional, Martens, A., additional, Roels, K., additional, De Lange, V., additional, Hoogewijs, M., additional, De Schauwer, C., additional, and Govaere, J., additional

Published
2015
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31. The FoalinMare: Insights inside the foaling mare – An animated educational tool for enhancing veterinary training

Author

Govaere, J, Martens, K, Van Haesebrouck, E, Hoogewijs, M, and de Kruif, A

Subjects
ddc: 610, 610 Medical sciences, Medicine
Abstract

Objectives: In comparison with other domestic animals, parturition in the horse is a fast and almost explosive event. Mostly it takes place in an uncomplicated manner, but when difficulties arise, adequate veterinary intervention can be life saving. Most of these life saving interventions can only be[for full text, please go to the a.m. URL], Jahrestagung der Gesellschaft für Medizinische Ausbildung - GMA

Published
2009
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44. Uterine Fibrosarcoma in a Warmblood Mare

Author

Govaere, J, primary, Maes, S, additional, Saey, V, additional, Blancke, W, additional, Hoogewijs, M, additional, Deschauwer, C, additional, Smits, K, additional, Roels, K, additional, Vercauteren, G, additional, and de Kruif, A, additional

Published
2010
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46. Birth of the first ICSI foal in the Benelux

Author

Smits, K., primary, Govaere, J., additional, De Schauwer, C., additional, van Haesebrouck, E., additional, Van Poucke, M., additional, Peelman, L.J., additional, Van den Berg, M., additional, Vullers, T., additional, and Van Soom, A., additional

Published
2010
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47. Ovarian teratoma in the mare: a review and two cases

Author

Vanhaesebrouck, E., primary, Govaere, J., additional, Smits, K., additional, Durie, I., additional, Vercauteren, G., additional, Martens, A., additional, Schauvlieghe, S., additional, Ducatelle, R., additional, Hoogewijs, M., additional, De Schauwer, C., additional, and de Kruif, A;, additional

Published
2010
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