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1. Corrigendum

Author

Dey, Nidhi S., Senaratne, Sujai, Somaratne, Vijani, Madarasinghe, Nayani P., Seneviratne, Bimalka, Forrester, Sarah, de Oca, Marcela Montes, Reis, Luiza Campos, Moulik, Srija, Walrad, Pegine B., Chatterjee, Mitali, Goto, Hiro, Wickremasinghe, Renu, Lagos, Dimitris, Kaye, Paul M., and Ranasinghe, Shalindra

Subjects
Health care industry
Abstract

Nidhi S. Dey, Sujai Senaratne, Vijani Somaratne, Nayani P. Madarasinghe, Bimalka Seneviratne, Sarah Forrester, Marcela Montes de Oca, Luiza Campos Reis, Srija Moulik, Pegine B. Walrad, Mitali Chatterjee, Hiro Goto, [...]

Published
2024
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2. ELISA with recombinant antigen Lb6H validated for the diagnosis of American tegumentary leishmaniasis

Author

Valencia-Portillo, Ruth Tamara, primary, Lindoso, José Angelo, additional, Celeste, Beatriz Julieta, additional, Bittencourt, Amanda Azevedo, additional, de Brito, Maria Edileuza Felinto, additional, Duthie, Malcolm Scott, additional, Guderian, Jeffery, additional, Guerra, Jorge, additional, Oliveira, Ana Lúcia Lyrio, additional, Reed, Steven, additional, Rocha, Mussya Cisotto, additional, Santos, Nicolle Tayná, additional, Silveira, Fernando Tobias, additional, Goto, Hiro, additional, and Sanchez, Maria Carmen Arroyo, additional

Published
2024
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3. Development of a Core Outcome Measure Instrument; "LeishCOM_LCL", for Localised Cutaneous Leishmaniasis

Author

Ranasinghe, Shalindra, primary, Senarathne, Sujai Senarathne, additional, Somaratne, Vijani Somaratne, additional, Lacey, Charles JN, additional, Jayakody, Surangi, additional, Wickramasinghe, Amila Wickramasinghe, additional, Kahawita, Indira, additional, Goto, Hiro, additional, Chatterjee, Mitali, additional, Lindoso, Jose AL, additional, Parkash, Vivak, additional, Chaudhuri, Surya, additional, Wickremasinghe, Renu, additional, Das, Nilay K, additional, Kaye, Paul M, additional, and Layton, Alison M, additional

Published
2024
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4. Development of a Core Outcome Measure Instrument; "LeishCOM_LCL", for Localised Cutaneous Leishmaniasis.

Author

Ranasinghe, Shalindra, Senarathne, Sujai, Somaratne, Vijani, Lacey, Charles J. N., Jayakody, Surangi, Wickramasinghe, Amila, Kahawita, Indira, Goto, Hiro, Chatterjee, Mitali, Lindoso, José A. L., Parkash, Vivak, Chaudhuri, Surya J., Wickremasinghe, Renu, Das, Nilay K., Kaye, Paul M., and Layton, Alison M.

Subjects
LITERATURE reviews, CUTANEOUS leishmaniasis, NOMINALS (Grammar), QUALITY of life, HYPERTROPHIC scars, KELOIDS
Abstract

Background: Localized cutaneous leishmaniasis (LCL) is a chronic ulcerating disease. A literature review identified inconsistencies in clinical trials. The aims of this study were to reach a consensus on the most important domains to measure when assessing LCL, agree on parameters to measure the domains, and develop a tool representing a Core Outcome Set (COS), for use in clinical assessment of LCL. Methodology & principal findings: A literature review was conducted to identify any existing COS for LCL embracing agreed Outcome Domains, i.e. what to measure and any Outcome Measurement Instruments (OMIs). As no COS was available, potential outcome domains for assessment of LCL were identified through an international collaborative approach using e-consultations and virtual discussions with expert stakeholders (n = 20) from geographically different LCL endemic countries. Subsequent judgmental validation process included a face-to-face multidisciplinary stakeholders' meeting adopting the Nominal Group Technique. A final consensual agreement on outcome domains and items required to measure these domains was established. "Clinical Cure" was defined as the ideal overall "General Concept". The five Core Outcome Domains included Signs capturing clinical morphology, diameter, and induration of an index lesion with the aid of a palpability score, Treatment Efficacy assessing percentage change in size of the lesion and re-epithelialization compared to baseline, Treatment Impact which included an investigator and patient visual analogue score, and Clinical Sequelae rating pigment change, atrophic and hypertrophic/keloid scars. It was agreed that two open-ended questions should be included to capture some aspects of Health-Related Quality of Life as a means of capturing a patient-focused approach. Conclusion: LeishCOM_LCL was generated to reflect a COS for LCL. This captured demographic details, agreed outcome domains and measures to assess these domains. Validation of LeishCOM_LCL will be reported in a separate paper. Development of a Patient Reported Outcome Measure will be considered in the future. Author summary: Localized cutaneous leishmaniasis (LCL) is a chronic ulcerating disease caused by the parasite Leishmania spp. Literature review identified inconsistencies in methods and parameters used to evaluate treatment/alternative-interventions resulting in difficulties in comparing new treatment/interventions in clinical trials. In our international consensual study, we adopted the face-to-face nominal group technique and a judgment process to identify domains key to assessment of LCL. Subsequent measures for each domain were used to form a Core Outcome Set (COS). LeishCOM_LCL was developed as an outcome measure instrument (OMI) to capture the COS incorporating existing and newly developed tools. "Clinical Cure" was agreed as the "General Concept" to be captured through five domains. It was agreed that "Signs" domain should capture clinical morphology, diameter, and induration of an index lesion with the aid of a palpability score. "Treatment Efficacy" was assessed by recording percentage change in size of the lesion and re-epithelialization compared to baseline. "Treatment Impact" was reflected through an investigator and the patient visual analogue score and "Clinical Sequelae" rated pigment change, atrophic/hypertrophic scars. Two open-ended questions were included to capture some aspects of "Health-Related Quality of Life". LeishCOM_LCL also records patient demographic details and was validated in a small cohort of patients. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Early reduction in PD-L1 expression predicts faster treatment response in human cutaneous leishmaniasis

Author

Dey, Nidhi S., primary, Senaratne, Sujai, additional, Somaratne, Vijani, additional, Madarasinghe, Nayani P., additional, Seneviratne, Bimalka, additional, Forrester, Sarah, additional, Montes de Oca, Marcela, additional, Reis, Luiza Campos, additional, Moulik, Srija, additional, Walrad, Pegine B., additional, Chatterjee, Mitali, additional, Goto, Hiro, additional, Wickremasinghe, Renu, additional, Lagos, Dimitris, additional, Kaye, Paul M., additional, and Ranasinghe, Shalindra, additional

Published
2024
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6. IL-32 producing CD8+memory T cells and Tregs define the IDO1 / PD-L1 niche in human cutaneous leishmaniasis skin lesions

Author

Dey, Nidhi S., primary, Dey, Shoumit, additional, Brown, Naj, additional, Senarathne, Sujai, additional, Reis, Luiza Campos, additional, Sengupta, Ritika, additional, Lindoso, Jose Angelo L., additional, James, Sally, additional, Gilbert, Lesley, additional, Chatterjee, Mitali, additional, Goto, Hiro, additional, Ranasinghe, Shalindra, additional, and Kaye, Paul M., additional

Published
2024
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7. Early reduction in PD-L1 expression predicts faster treatment response in human cutaneous leishmaniasis

Author

Dey, Nidhi S., Senaratne, Sujai, Somaratne, Vijani, Madarasinghe, Nayani P., Seneviratne, Bimalka, Forrester, Sarah, de Oca, Marcela Montes, Reis, Luiza Campos, Moulik, Srija, Walrad, Pegine B., Chatterjee, Mitali, Goto, Hiro, Wickremasinghe, Renu, Lagos, Dimitris, Kaye, Paul M., and Ranasinghe, Shalindra

Subjects
Leishmaniasis, Cutaneous -- Care and treatment -- Genetic aspects, Immunotherapy -- Methods, Membrane proteins -- Health aspects -- Genetic aspects, Health care industry
Abstract

Cutaneous leishmaniasis (CL) is caused by Leishmania donovani in Sri Lanka. Pentavalent antimonials (e.g., sodium stibogluconate [SSG]) remain first-line drugs for CL with no new effective treatments emerging. We studied whole blood and lesion transcriptomes from Sri Lankan patients with CL at presentation and during SSG treatment. From lesions but not whole blood, we identified differential expression of immune-related genes, including immune checkpoint molecules, after onset of treatment. Using spatial profiling and RNA-FISH, we confirmed reduced expression of programmed death-ligand 1 (PD-L1) and indoleamine 2,3-dioxygenase 1 (IDO1) proteins on treatment in lesions of a second validation cohort and further demonstrated significantly higher expression of these checkpoint molecules on parasite- infected compared with noninfected lesional [CD68.sup.+] monocytes and macrophages. Crucially, early reduction in PD-L1 but not IDO1 expression was predictive of rate of clinical cure (HR = 4.88) and occurred in parallel with reduction in parasite load. Our data support a model whereby the initial antileishmanial activity of antimonial drugs alleviates checkpoint inhibition on T cells, facilitating immune-drug synergism and clinical cure. Our findings demonstrate that PD-L1 expression can be used as a predictor of rapidity of clinical response to SSG treatment in Sri Lanka and support further evaluation of PD-L1 as a host- directed therapeutic in leishmaniasis., Introduction Every year, approximately 600,000 to 1 million new cases of cutaneous leishmaniasis (CL) occur, with a broad global distribution, often leading to stigma and reduced life chances and placing [...]

Published
2021
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8. Recombinant protein KR95 as an alternative for serological diagnosis of human visceral leishmaniasis in the Americas

Author

Fujimori, Mahyumi, primary, Valencia-Portillo, Ruth Tamara, additional, Lindoso, José Angelo Lauletta, additional, Celeste, Beatriz Julieta, additional, de Almeida, Roque Pacheco, additional, Costa, Carlos Henrique Nery, additional, da Cruz, Alda Maria, additional, Druzian, Angelita Fernandes, additional, Duthie, Malcolm Scott, additional, Fortaleza, Carlos Magno Castelo Branco, additional, Oliveira, Ana Lúcia Lyrio de, additional, Paniago, Anamaria Mello Miranda, additional, Queiroz, Igor Thiago, additional, Reed, Steve, additional, Vallur, Aarthy C., additional, Goto, Hiro, additional, and Sanchez, Maria Carmen Arroyo, additional

Published
2023
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9. Staphylococcus aureus-Cure-Associated Antigens Elicit Type 3 Immune Memory T Cells

Author

Santos, Kamila R., primary, Souza, Fernando N., additional, Ramos-Sanchez, Eduardo M., additional, Batista, Camila F., additional, Reis, Luiza C., additional, Fotoran, Wesley L., additional, Heinemann, Marcos B., additional, Cunha, Adriano F., additional, Rocha, Mussya C., additional, Faria, Angélica R., additional, Andrade, Hélida M., additional, Cerqueira, Mônica M. O. P., additional, Gidlund, Magnus, additional, Goto, Hiro, additional, and Della Libera, Alice Maria M. P., additional

Published
2022
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10. Memory CD4+ and CD8+ T lymphocyte proliferation in vaccinated dairy cows with different histories of Staphylococcus aureus mastitis

Author

Soares, Thais C.S., primary, Santos, Kamila R., additional, Lima, Daniel M., additional, Maia, Raysa Brenda M., additional, Ramos-Sanchez, Eduardo M., additional, Reis, Luiza C., additional, Gidlund, Magnus, additional, da Cunha, Adriano F., additional, Ordinola-Ramirez, Carla M., additional, Cerqueira, Mônica M.O.P., additional, Heinemann, Marcos B., additional, Della Libera, Alice M.M.P., additional, Goto, Hiro, additional, and Souza, Fernando N., additional

Published
2022
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11. Complementary Role of Computer Aided Detection in Mammography

Author

Sugisaki, Keiko, Fujita, Hiroshi, Goto, Hiro, Hoshi, Hiroaki, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Pandu Rangan, C., editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Dough, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, Astley, Susan M., editor, Brady, Michael, editor, Rose, Chris, editor, and Zwiggelaar, Reyer, editor

Published
2006
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16. Corrigendum: miR-548d-3p Is Up-regulated in Human Visceral Leishmaniasis and Suppresses Parasite Growth in Macrophages

Author

Ramos-Sanchez, Eduardo Milton, primary, Reis, Luiza Campos, additional, Souza, Marina de Assis, additional, Muxel, Sandra Márcia, additional, Santos, Kamila Reis, additional, Lagos, Dimitris, additional, Pereira, Valéria Rêgo Alves, additional, de Brito, Maria Edileuza Felinto, additional, Kaye, Paul Martin, additional, Floeter-Winter, Lucile Maria, additional, and Goto, Hiro, additional

Published
2022
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17. miR-548d-3p Is Up-Regulated in Human Visceral Leishmaniasis and Suppresses Parasite Growth in Macrophages

Author

Ramos-Sanchez, Eduardo Milton, primary, Reis, Luiza Campos, additional, Souza, Marina de Assis, additional, Muxel, Sandra Márcia, additional, Santos, Kamila Reis, additional, Lagos, Dimitris, additional, Pereira, Valéria Rêgo Alves, additional, Brito, Maria Edileuza Felinto de, additional, Kaye, Paul Martin, additional, Floeter-Winter, Lucile Maria, additional, and Goto, Hiro, additional

Published
2022
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18. Wide visible-range activatable fluorescence ZnSe:Eu3+/Mn2+@ZnS quantum dots: local atomic structure order and application as a nanoprobe for bioimaging

Author

Khan, Zahid Ullah, primary, Uchiyama, Mayara Klimuk, additional, Khan, Latif Ullah, additional, Araki, Koiti, additional, Goto, Hiro, additional, Felinto, Maria Claudia França Cunha, additional, de Souza, Ana Olivia, additional, de Brito, Hermi Felinto, additional, and Gidlund, Magnus, additional

Published
2022
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23. Staphylococcus aureus Protection-Related Type 3 Cell-Mediated Immune Response Elicited by Recombinant Proteins and GM-CSF DNA Vaccine

Author

Santos, Kamila R., primary, Souza, Fernando N., additional, Ramos-Sanchez, Eduardo M., additional, Batista, Camila F., additional, Reis, Luiza C., additional, Fotoran, Wesley F., additional, Heinemann, Marcos B., additional, Goto, Hiro, additional, Gidlund, Magnus, additional, Cunha, Adriano F., additional, Faria, Angélica Rosa, additional, Andrade, Hélida M., additional, Lage, Andrey P., additional, Cerqueira, Mônica M. O. P., additional, and Della Libera, Alice M. M. P., additional

Published
2021
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25. Pleiotropic Effect of Hormone Insulin-Like Growth Factor-I in Immune Response and Pathogenesis in Leishmaniases

Author

Reis, Luiza C., Ramos-Sanchez, Eduardo Milton, Araujo, Fernanda N., Leal, Ariane F., Ozaki, Christiane Y., Sevillano, Orlando R., Uscata, Bernardina A., and Goto, Hiro

Subjects
Article Subject
Abstract

Leishmaniases are diseases caused by several Leishmania species, and many factors contribute to the development of the infection. Because the adaptive immune response does not fully explain the outcome of Leishmania infection and considering that the initial events are crucial in the establishment of the infection, we investigated one of the growth factors, the insulin-like growth factor-I (IGF-I), found in circulation and produced by different cells including macrophages and present in the skin where the parasite is inoculated. Here, we review the role of IGF-I in leishmaniasis experimental models and human patients. IGF-I induces the growth of different Leishmania species in vitro and alters the disease outcome increasing the parasite load and lesion size, especially in L. major- and L. amazonensis-infected mouse leishmaniasis. IGF-I affects the parasite interacting with the IGF-I receptor present on Leishmania. During Leishmania-macrophage interaction, IGF-I acts on the arginine metabolic pathway, resulting in polyamine production both in macrophages and Leishmania. IGF-I and cytokines interact with reciprocal influences on their expression. IL-4 is a hallmark of susceptibility to L. major in murine leishmaniasis, but we observed that IGF-I operates astoundingly as an effector element of the IL-4. Approaching human leishmaniasis, patients with mucosal, disseminated, and visceral diseases presented surprisingly low IGF-I serum levels, suggesting diverse effects than parasite growth. We observed that low IGF-I levels might contribute to the inflammatory response persistence and delayed lesion healing in human cutaneous leishmaniasis and the anemia development in visceral leishmaniasis. We must highlight the complexity of infection revealed depending on the Leishmania species and the parasite’s developmental stages. Because IGF-I exerts pleiotropic effects on the biology of interaction and disease pathogenesis, IGF-I turns up as an attractive tool to explore biological and pathogenic processes underlying infection development. IGF-I pleiotropic effects open further the possibility of approaching IGF-I as a therapeutical target.

Published
2021
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26. Unusual manifestation of genital cutaneous leishmaniasis in an immunocompetent patient from São Paulo, Brazil: A case report

Author

Reis, Luiza Campos, Lindoso, José Angelo Lauletta, Celeste, Beatriz Julieta, Braz, Lucia Maria Almeida, Ramos-Sanchez, Eduardo Milton, Yamashiro-Kanashiro, Edite Hatsumi, Goto, Hiro, and Oyafuso, Luiza Keiko Matsuka

Subjects
Cutaneous leishmaniasis, Genital lesion, Diagnosis
Abstract

A 31-year-old male patient developed an ulcer on the glans penis that evolved for three months without healing. We diagnosed it as leishmaniasis using polymerase chain reaction. No immunosuppression or associated diseases were observed. The patient was treated with meglumine antimoniate that cured the lesion in a month post-treatment. Here, we report this case of cutaneous leishmaniasis lesion at the unusual location of glans penis in an immunocompetent individual. The lesion likely developed due to the bite of a vector, highlighting the need for considering cutaneous leishmaniasis among differential diagnosis of sexually transmitted diseases in areas endemic for leishmaniasis.

Published
2021

27. miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection

Author

Souza, Marina de Assis, primary, Ramos-Sanchez, Eduardo Milton, additional, Muxel, Sandra Márcia, additional, Lagos, Dimitris, additional, Reis, Luiza Campos, additional, Pereira, Valéria Rêgo Alves, additional, Brito, Maria Edileuza Felinto, additional, Zampieri, Ricardo Andrade, additional, Kaye, Paul Martin, additional, Floeter-Winter, Lucile Maria, additional, and Goto, Hiro, additional

Published
2021
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28. Improved Performance of ELISA and Immunochromatographic Tests Using a New Chimeric A2-Based Protein for Human Visceral Leishmaniasis Diagnosis

Author

Figueiredo, Maria Marta, primary, dos Santos, Anna R. R., additional, Godoi, Lara C., additional, de Castro, Natália S., additional, de Andrade, Bruno C., additional, Sergio, Sarah A. R., additional, Jerônimo, Selma M. B., additional, de Oliveira, Edward J., additional, Valencia-Portillo, Ruth T., additional, Bezerra, Lucilândia M., additional, Goto, Hiro, additional, Sanchez, Maria C. A., additional, Junqueira, Caroline, additional, Teixeira, Santuza M. R., additional, da Fonseca, Flávio G., additional, Gazzinelli, Ricardo T., additional, and Fernandes, Ana Paula, additional

Published
2021
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30. Staphylococcus Aureus- Cure Associated Antigens Elicit Type 3 Immune Memory T Cells

Author

Santos, Kamila Reis, primary, Souza, Fernando, additional, Sanchez, Eduardo, additional, Batista, Camila, additional, Reis, Luiza, additional, Fotoran, Wesley, additional, Heinemann, Marcos, additional, Cunha, Adriano, additional, Rocha, Mussya, additional, Faria, Angélica, additional, Andrade, Hélida, additional, Cerqueira, Mônica, additional, Gidlund, Magnus, additional, Goto, Hiro, additional, and Della Libera, Alice, additional

Published
2021
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32. Validation of ELISA with recombinant antigens in serological diagnosis of canine Leishmania infantum infection

Author

Fujimori, Mahyumi, primary, de Almeida, Arleana do Bom Parto Ferreira, additional, Barrouin-Melo, Stella Maria, additional, Cortez, Luiz Ricardo Paes de Barros, additional, Duthie, Malcolm Scott, additional, Hiramoto, Roberto Mitsuyoshi, additional, de Pinho, Flaviane Alves, additional, Reed, Steven Gregory, additional, Sousa, Valéria Régia Franco, additional, Souza, Nazaré Fonseca, additional, Soares, Rodrigo Martins, additional, Tolezano, José Eduardo, additional, Sanchez, Maria Carmen Arroyo, additional, and Goto, Hiro, additional

Published
2021
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34. Reduced expression of PD-L1 and IDO1 characterises early response to antimonial therapy in cutaneous leishmaniasis patients

Author

Dey, Nidhi S., Senarathna, Sujai, Somaratne, Vijani, Madarasinghe, Nayani, Seneviratne, Bimalka, Reis, Luiza Campos, Moulik, Srija, Walrad, Pegine, Chatterjee, Mitali, Goto, Hiro, Wickremasinghe, Renu, Lagos, Dimitris, Kaye, Paul M., and Ranasinghe, Shalindra

Abstract

Cutaneous leishmaniasis (CL) is a disfiguring disease caused by infection with Leishmania parasites and is characterised by parasitism of the dermis and chronic inflammation. Whilst T cell responses to Leishmania are essential for both parasite clearance and disease resolution they also drive inflammation, and clinical presentation reflects the balance of these opposing activities 1 . Pentavalent antimonials (e.g. sodium stibogluconate; SSG) remain the first line drugs for CL, even though treatment may be protracted and painful. Although evidence from animal models indicates that an effective clinical response to antimonials requires immune-drug synergy 2 , little is known about how this operates in human disease. Here, we studied formalin fixed paraffin embedded (FFPE) skin biopsies from patients in Sri Lanka with CL, at presentation and during intra-lesional SSG treatment. Immune-targeted transcriptomics in a test patient cohort indicated heightened immune checkpoint pathway expression at presentation. We confirmed reduced PD-L1 and IDO1 protein expression on treatment in a second validation cohort, using digital spatial profiling and quantitative immunohistochemistry. PD-L1 and IDO1 expression on CD68 + monocytes / macrophages was positively correlated with the degree of intracellular parasitism, as determined by parasite-specific RNA FISH. Our data support a model whereby the initial anti-leishmanial activity of antimonial drugs alleviates checkpoint inhibition of T cell immunity, thus facilitating immune-drug synergism and clinical cure. We suggest a need to evaluate shorter course SSG treatment and/or the use of checkpoint inhibition as an adjunct host directed therapy (HDT) in CL.

Published
2020
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36. Dual Host-Intracellular Parasite Transcriptome of Enucleated Cells Hosting Leishmania amazonensis : Control of Half-Life of Host Cell Transcripts by the Parasite

Author

Orikaza, Cristina M., primary, Pessoa, Carina C., additional, Paladino, Fernanda V., additional, Florentino, Pilar T. V., additional, Barbiéri, Clara L., additional, Goto, Hiro, additional, Ramos-Sanchez, Eduardo Milton, additional, Franco da Silveira, José, additional, Rabinovitch, Michel, additional, Mortara, Renato A., additional, and Real, Fernando, additional

Published
2020
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37. Wide visible-range activatable fluorescence ZnSe:Eu3+/Mn2+@ZnS quantum dots: local atomic structure order and application as a nanoprobe for bioimaging.

Author

Khan, Zahid Ullah, Uchiyama, Mayara Klimuk, Khan, Latif Ullah, Araki, Koiti, Goto, Hiro, Felinto, Maria Claudia França Cunha, de Souza, Ana Olivia, de Brito, Hermi Felinto, and Gidlund, Magnus

Abstract

The development of QDs-based fluorescent bionanoprobe for cellular imaging fundamentally relies upon the precise knowledge of particle–cell interaction, optical properties of QDs inside and outside of the cell, movement of a particle in and out of the cell, and the fate of particle. We reported engineering and physicochemical characterization of water-dispersible Eu3+/Mn2+ co-doped ZnSe@ZnS core/shell QDs and studied their potential as a bionanoprobe for biomedical applications, evaluating their biocompatibility, fluorescence behaviour by CytoViva dual mode fluorescence imaging, time-dependent uptake, endocytosis and exocytosis in RAW 264.7 macrophages. The oxidation state and local atomic structure of the Eu dopant studied by X-ray absorption fine structure (XAFS) analysis manifested that the Eu3+ ions occupied sites in both ZnSe and ZnS lattices for the core/shell QDs. A novel approach was developed to relieve the excitation constraint of wide bandgap ZnSe by co-incorporation of Eu3+/Mn2+ codopants, enabling the QDs to be excited at a wide UV-visible range. The QDs displayed tunable emission colors by a gradual increase in Eu3+ concentration at a fixed amount of Mn2+, systematically enhancing the Mn2+ emission intensity via energy transfer from the Eu3+ to Mn2+ ion. The ZnSe:Eu3+/Mn2+@ZnS QDs presented high cell viability above 85% and induced no cell activation. The detailed analyses of QDs-treated cells by dual mode fluorescence CytoViva microscopy confirmed the systematic color-tunable fluorescence and its intensity enhances as a function of incubation time. The QDs were internalized by the cells predominantly via macropinocytosis and other lipid raft-mediated endocytic pathways, retaining an efficient amount for 24 h. The unique color tunability and consistent high intensity emission make these QDs useful for developing a multiplex fluorescent bionanoprobe, activatable in wide-visible region. [ABSTRACT FROM AUTHOR]

Published
2022
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38. Orange-Emitting ZnSe:Mn2+ Quantum Dots as Nanoprobes for Macrophages

Author

Khan, Zahid U., primary, Uchiyama, Mayara K., additional, Khan, Latif U., additional, Ramos-Sanchez, Eduardo M., additional, Reis, Luiza Campos, additional, Nakamura, Marcelo, additional, Goto, Hiro, additional, De Souza, Ana O., additional, Araki, Koiti, additional, Brito, Hermi F., additional, and Gidlund, Magnus, additional

Published
2020
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39. Mathematical Modelling Using Predictive Biomarkers for the Outcome of Canine Leishmaniasis upon Chemotherapy

Author

de Sousa Gonçalves, Rafaela, primary, Alves de Pinho, Flaviane, additional, Dinis-Oliveira, Ricardo Jorge, additional, Azevedo, Rui, additional, Gaifem, Joana, additional, Farias Larangeira, Daniela, additional, Ramos-Sanchez, Eduardo Milton, additional, Goto, Hiro, additional, Silvestre, Ricardo, additional, and Barrouin-Melo, Stella Maria, additional

Published
2020
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40. Correction: Performance of rK39-based immunochromatographic rapid diagnostic test for serodiagnosis of visceral leishmaniasis using whole blood, serum and oral fluid

Author

Sanchez, Maria Carmen Arroyo, primary, Celeste, Beatriz Julieta, additional, Lindoso, José Angelo Lauletta, additional, Fujimori, Mahyumi, additional, de Almeida, Roque Pacheco, additional, Fortaleza, Carlos Magno Castelo Branco, additional, Druzian, Angelita Fernandes, additional, Lemos, Ana Priscila Freitas, additional, de Melo, Vanessa Campos Andrade, additional, Miranda Paniago, Anamaria Mello, additional, Queiroz, Igor Thiago, additional, and Goto, Hiro, additional

Published
2020
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41. Performance of rK39-based immunochromatographic rapid diagnostic test for serodiagnosis of visceral leishmaniasis using whole blood, serum and oral fluid

Author

Sanchez, Maria Carmen Arroyo, primary, Celeste, Beatriz Julieta, additional, Lindoso, José Angelo Lauletta, additional, Fujimori, Mahyumi, additional, de Almeida, Roque Pacheco, additional, Fortaleza, Carlos Magno Castelo Branco, additional, Druzian, Angelita Fernandes, additional, Lemos, Ana Priscila Freitas, additional, de Melo, Vanessa Campos Andrade, additional, Miranda Paniago, Anamaria Mello, additional, Queiroz, Igor Thiago, additional, and Goto, Hiro, additional

Published
2020
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42. Early reduction in PD-L1 expression predicts faster treatment response in human cutaneous leishmaniasis

Author

Dey, Nidhi S., primary, Senarathna, Sujai, additional, Somaratne, Vijani, additional, Madarasinghe, Nayani, additional, Seneviratne, Bimalka, additional, Forrester, Sarah, additional, Oca, Marcela Montes De, additional, Reis, Luiza Campos, additional, Moulik, Srija, additional, Walrad, Pegine, additional, Chatterjee, Mitali, additional, Goto, Hiro, additional, Wickremasinghe, Renu, additional, Lagos, Dimitris, additional, Kaye, Paul M., additional, and Ranasinghe, Shalindra, additional

Published
2020
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47. CD4+ 1 T cells alter the stromal microenvironment and repress medullary erythropoiesis in murine visceral leishmaniasis

Author

Preham, Olivier Yvon Giuseppe, Pinho, Flaviane Alves, Pinto, Ana Isabel, Rani, Gulab Fatima, Brown, Najmeeyah, Hitchcock, Ian Stuart, Goto, Hiro, and Kaye, Paul

Abstract

Human visceral leishmaniasis, a parasitic disease of major public health importance in developing countries, is characterized by variable degrees of severity of anemia, but the mechanisms underlying this change in peripheral blood have not been thoroughly explored. Here, we used an experimental model of visceral leishmaniasis in C57BL/6 mice to explore the basis of anemia following infection with Leishmania donovani. 28 days post infection,mice showed bone marrow dyserythropoiesis by myelogram, with a reduction of TER119+ CD71-/+ erythroblasts. Reduction of medullary erythropoiesis coincided with loss of CD169high bone marrow stromal macrophages and a reduction of CXCL12-expressing stromal cells. Although the spleen is a site of extramedullary erythropoiesis and erythrophagocytosis, splenectomy did not impact the extent of anemia or affect the repression of medullary hematopoiesis that was observed in infected mice. In contrast, these changes in bone marrow erythropoiesis were not evident in B6.Rag2 -/- mice, but could be fully reconstituted by adoptive transfer of IFNg-producing but not IFNg-deficient CD4+ T cells, mimicking the expansion of IFNg-producing CD4+ T cells that occurs during infection in wild type mice. Collectively, these data indicate that anemia during experimental murine visceral leishmaniasis can be driven by defects associated with the bone marrow erythropoietic niche, and that this represents a further example of CD4+ T cell-mediated immunopathology affecting hematopoietic competence.

Published
2018

49. Macrophages From Subjects With Isolated GH/IGF-I Deficiency Due to a GHRH Receptor Gene Mutation Are Less Prone to Infection by Leishmania amazonensis

Author

Barrios, Mônica R., primary, Campos, Viviane C., additional, Peres, Nalu T. A., additional, de Oliveira, Laís L., additional, Cazzaniga, Rodrigo A., additional, Santos, Márcio B., additional, Aires, Murilo B., additional, Silva, Ricardo L. L., additional, Barreto, Aline, additional, Goto, Hiro, additional, Almeida, Roque P., additional, Salvatori, Roberto, additional, Aguiar-Oliveira, Manuel H., additional, and Jesus, Amélia M. R., additional

Published
2019
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50. ATP6V0d2 controls Leishmania parasitophorous vacuole biogenesis via cholesterol homeostasis

Author

Pessoa, Carina Carraro, primary, Reis, Luiza Campos, additional, Ramos-Sanchez, Eduardo Milton, additional, Orikaza, Cristina Mary, additional, Cortez, Cristian, additional, de Castro Levatti, Erica Valadares, additional, Badaró, Ana Carolina Benites, additional, Yamamoto, Joyce Umbelino da Silva, additional, D’Almeida, Vânia, additional, Goto, Hiro, additional, Mortara, Renato Arruda, additional, and Real, Fernando, additional

Published
2019
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