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1. Rapid decline of neutralizing antibodies against SARS-CoV-2 among infected healthcare workers

Author

Stéphane Marot, Isabelle Malet, Valentin Leducq, Karen Zafilaza, Delphine Sterlin, Delphine Planas, Adélie Gothland, Aude Jary, Karim Dorgham, Timothée Bruel, the Sorbonne Université SARS-CoV-2 Neutralizing Antibodies Study Group, Sonia Burrel, David Boutolleau, Olivier Schwartz, Guy Gorochov, Vincent Calvez, and Anne-Geneviève Marcelin

Subjects
Science
Abstract

The humoral immune response to SARS-CoV-2 infection is not yet fully understood. Here, Marot et al. monitor the longitudinal profile and neutralizing activity of IgG, IgA, and IgM among 26 healthcare workers and provide evidence for a short-lasting humoral immune protection due to a decrease of neutralizing antibody titers within 3 months.

Published
2021
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2. Harnessing Redox Disruption to Treat Human Herpesvirus 8 (HHV-8) Related Malignancies

Author

Adélie Gothland, Aude Jary, Philippe Grange, Valentin Leducq, Laurianne Beauvais-Remigereau, Nicolas Dupin, Anne-Geneviève Marcelin, and Vincent Calvez

Subjects
HHV-8, Kaposi sarcoma, primary effusion lymphoma, metabolism, oxidoreduction disruption, oncogenesis, Therapeutics. Pharmacology, RM1-950
Abstract

Reprogrammed metabolism is regarded as a hallmark of cancer and offers a selective advantage to tumor cells during carcinogenesis. The redox equilibrium is necessary for growth, spread and the antioxidant pathways are boosted following Reactive Oxygen Species (ROS) production to prevent cell damage in tumor cells. Human herpesvirus 8 (HHV-8), the etiologic agent of Kaposi sarcoma KS and primary effusion lymphoma (PEL), is an oncogenic virus that disrupts cell survival-related molecular signaling pathways leading to immune host evasion, cells growths, angiogenesis and inflammatory tumor-environment. We recently reported that primaquine diphosphate causes cell death by apoptosis in HHV-8 infected PEL cell lines in vivo and exhibits therapeutic anti-tumor activity in mice models and advanced KS. Our findings also suggest that the primaquine-induced apoptosis in PEL cells is mostly influenced by ROS production and targeting the redox balance could be a new approach to treat HHV-8 related diseases. In this review, we summarized the knowledge about the influence of ROS in cancer development; more specifically, the proof of evidence from our work and from the literature that redox pathways are important for the development of HHV-8 pathologies.

Published
2022
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3. Sotrovimab therapy elicits antiviral activities against Omicron BQ.1.1 and XBB.1.5 in sera of immunocompromised patients

Author

Bruel, Timothée, primary, Vrignaud, Lou-Léna, additional, Porrot, Françoise, additional, Staropoli, Isabelle, additional, Planas, Delphine, additional, Guivel-Benhassine, Florence, additional, Puech, Julien, additional, Prot, Matthieu, additional, Munier, Sandie, additional, Bolland, William Henry, additional, Soulié, Cathia, additional, Zafilaza, Karen, additional, Lusivika-Nzinga, Clovis, additional, Meledge, Marie-Laure, additional, Dorival, Céline, additional, Molino, Diana, additional, Péré, Hélène, additional, Yordanov, Youri, additional, Simon-Lorière, Etienne, additional, Veyer, David, additional, Carrat, Fabrice, additional, Schwartz, Olivier, additional, Marcelin, Anne-Geneviève, additional, Martin-Blondel, Guillaume, additional, Garcia, Magalie, additional, Giraud, Valentin, additional, Metais, Agathe, additional, Cazenave-Roblot, Fance, additional, Martellosio Anne-Marie Ronchetti, Jean-Philippe, additional, Gabas, Thomas, additional, Hadjadj, Naima, additional, Salanoubat, Célia, additional, Chabrol, Amélie, additional, Housset, Pierre, additional, Pardon, Agathe, additional, Faucon, Anne-Laure, additional, Caudwell, Valérie, additional, Roland Liblau, Latifa Hanafi, additional, Alric, Laurent, additional, Pugnet, Grégory, additional, Mourguet, Morgane, additional, Bories, Eva, additional, Bonnet, Delphine, additional, Charpentier, Sandrine, additional, Delobel, Pierre, additional, Debard, Alexa, additional, Beck, Colleen, additional, Boumaza, Xavier, additional, Fanny Lanternier, Stella Rousset, additional, Delage, Claire, additional, Pires, Elisabete Gomes, additional, Cheminant, Morgane, additional, Anthony Chauvin, Nathalie Chavarot, additional, Véronique Delcey Simon Bessis, Xavier Eyer, additional, Cresta, Mélanie, additional, Pélagie Thibaut, Romain Gueneau, additional, Nadal, Marine, additional, Siguier, Martin, additional, Bachir, Marwa, additional, Valérie Pourcher, Christia Palacios, additional, Melenotte, Cléa, additional, Faycal, Antoine, additional, Berot, Vincent, additional, Brin, Cécile, additional, Djebara, Siham, additional, Marot, Stéphane, additional, Sayon, Sophie, additional, Leducq, Vincent, additional, Malet, Isabelle, additional, Teyssou, Elisa, additional, Karine Lacombe, Adélie Gothland, additional, Aad, Yasmine Abi, additional, Chiarabini, Thibault, additional, Feliho, Raynald, additional, Valin, Nadia, additional, Brigant, Fabien, additional, Boize, Julien, additional, Thiébaud, Pierre-Clément, additional, Moreau, Marie, additional, Nathalie De Castro, Charlotte Billard, additional, Liégeon, Geoffroy, additional, Denis, Blandine, additional, Molina, Jean-Michel, additional, André Cabié, Lucia Etheve, additional, Abel, Sylvie, additional, Cabras, Ornella, additional, Guitteaud, Karine, additional, François Vincent Dubee, Sandrine Pierre-, additional, Ndiaye, Diama, additional, Pehlivan, Jonathan, additional, Phelippeau, Michael, additional, Alexandre Duvignaud, Rafael Mahieu, additional, Pistone, Thierry, additional, Desclaux, Arnaud, additional, Neau, Didier, additional, Jean-François Faucher, Charles Cazanave, additional, Festou, Benjamin, additional, Dupuy-Grasset, Magali, additional, Loustaud-Ratti, Véronique, additional, Nathan Peiffer- Smadja, Delphine Chainier, additional, Choquet, Christophe, additional, Da Conceicao, Olivia, additional, Thy, Michael, additional, Collas, Lio, additional, Godard, Cindy, additional, Bouzid, Donia, additional, Ing, Vittiaroat, additional, Pereira, Laurent, additional, Pavlowsky, Thomas, additional, RavautAntoine Asquier-Khati, Camille, additional, Boutoille, David, additional, Chauveau, Marie, additional, Deschanvres, Colin, additional, Audrey Le Bot, François Raffi, additional, Cailleaux, Marine, additional, Benezit, François, additional, Maillard, Anne, additional, Hue, Benoit, additional, François Coustilleres, Pierre Tattevin, additional, Carvalho-Schneider, Claudia, additional, Jamard, Simon, additional, Petit, Laeticia, additional, Natacha Mrozek, Karl Stefic, additional, Theis, Clément, additional, Vidal, Magali, additional, Sauvat, Léo, additional, Benjamin Lefèvre, Delphine Martineau, additional, Baronnet, Guillaume, additional, Florence Ader, Agnès Didier, additional, Perpoint, Thomas, additional, Conrad, Anne, additional, Chabert, Paul, additional, Aurélie Martin, Pierre Chauvelot, additional, Loubet, Paul, additional, Mazet, Julien, additional, Larcher, Romaric, additional, Mathilde Devaux Jérôme Frey, Didier Laureillard, additional, Woerlen, Amos, additional, Remillon, Aline, additional, Absensur-Vuillaume, Laure, additional, Albert Trinh-Duc, Pauline Bouquet, additional, Philippe Petua, Patrick Rispal, additional, Aurore Perrot, Julien Carillo, additional, Delavigne, Karen, additional, Cougoul, Pierre, additional, Dion, Jérémie, additional, Mathieu Blot, Odile Rauzy, additional, Sixt, Thibault, additional, Moretto, Florian, additional, Charles, Carole, additional, Sophie Circosta, Lionel Piroth, additional, Leger, Lydia, additional, Arulananthan, Arulvani, additional, Lascoux, Carine, additional, Valérie, Pascaline, additional, Yazdan Yazdanpanah, Leia Becam, additional, Petrov-Sanchez, Ventzislava, additional, Diallo, Alpha, additional, Le Mestre, Soizic, additional, Le Meut Isabelle Goderel, Guillaume, additional, Chau, Frédéric, additional, Soltana, Brahim, additional, Chane Tang, Jessica, additional, Guedj, Jérémie, additional, and Caille, Yvanie, additional

Published
2023
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5. SARS-CoV-2 variant-dependent inflammasome activation

Author

Teyssou, Elisa, primary, Marot, Stephane, additional, Gothland, Adelie, additional, Malet, Isabelle, additional, Zafilaza, Karen, additional, Leducq, Valentin, additional, Cocherie, Theophile, additional, Todesco, Eve, additional, Soulie, Cathia, additional, Marcelin, Anne Geneviève, additional, and Calvez, Vincent, additional

Published
2023
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6. Author Correction: Rapid decline of neutralizing antibodies against SARS-CoV-2 among infected healthcare workers

Author

Stéphane Marot, Isabelle Malet, Valentin Leducq, Karen Zafilaza, Delphine Sterlin, Delphine Planas, Adélie Gothland, Aude Jary, Karim Dorgham, Timothée Bruel, the Sorbonne Université SARS-CoV-2 Neutralizing Antibodies Study Group, Sonia Burrel, David Boutolleau, Olivier Schwartz, Guy Gorochov, Vincent Calvez, and Anne-Geneviève Marcelin

Subjects
Science
Published
2021
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7. Sotrovimab therapy elicits antiviral activities against Omicron BQ.1.1 and XBB.1.5 in sera of immunocompromised patients

Author

Garcia, Magalie, Giraud, Valentin, Metais, Agathe, Cazenave-Roblot, Fance, Martellosio Anne-Marie Ronchetti, Jean-Philippe, Gabas, Thomas, Hadjadj, Naima, Salanoubat, Célia, Chabrol, Amélie, Housset, Pierre, Pardon, Agathe, Faucon, Anne-Laure, Caudwell, Valérie, Roland Liblau, Latifa Hanafi, Alric, Laurent, Pugnet, Grégory, Mourguet, Morgane, Bories, Eva, Bonnet, Delphine, Charpentier, Sandrine, Delobel, Pierre, Debard, Alexa, Beck, Colleen, Boumaza, Xavier, Fanny Lanternier, Stella Rousset, Delage, Claire, Pires, Elisabete Gomes, Cheminant, Morgane, Anthony Chauvin, Nathalie Chavarot, Véronique Delcey Simon Bessis, Xavier Eyer, Cresta, Mélanie, Pélagie Thibaut, Romain Gueneau, Nadal, Marine, Siguier, Martin, Bachir, Marwa, Valérie Pourcher, Christia Palacios, Melenotte, Cléa, Faycal, Antoine, Berot, Vincent, Brin, Cécile, Djebara, Siham, Zafilaza, Karen, Marot, Stéphane, Sayon, Sophie, Leducq, Vincent, Malet, Isabelle, Teyssou, Elisa, Karine Lacombe, Adélie Gothland, Aad, Yasmine Abi, Chiarabini, Thibault, Feliho, Raynald, Valin, Nadia, Brigant, Fabien, Boize, Julien, Thiébaud, Pierre-Clément, Moreau, Marie, Nathalie De Castro, Charlotte Billard, Liégeon, Geoffroy, Denis, Blandine, Molina, Jean-Michel, André Cabié, Lucia Etheve, Abel, Sylvie, Cabras, Ornella, Guitteaud, Karine, François Vincent Dubee, Sandrine Pierre, Ndiaye, Diama, Pehlivan, Jonathan, Phelippeau, Michael, Alexandre Duvignaud, Rafael Mahieu, Pistone, Thierry, Desclaux, Arnaud, Neau, Didier, Jean-François Faucher, Charles Cazanave, Festou, Benjamin, Dupuy-Grasset, Magali, Loustaud-Ratti, Véronique, Nathan Peiffer- Smadja, Delphine Chainier, Choquet, Christophe, Da Conceicao, Olivia, Thy, Michael, Collas, Lio, Godard, Cindy, Bouzid, Donia, Ing, Vittiaroat, Pereira, Laurent, Pavlowsky, Thomas, RavautAntoine Asquier-Khati, Camille, Boutoille, David, Chauveau, Marie, Deschanvres, Colin, Audrey Le Bot, François Raffi, Cailleaux, Marine, Benezit, François, Maillard, Anne, Hue, Benoit, François Coustilleres, Pierre Tattevin, Carvalho-Schneider, Claudia, Jamard, Simon, Petit, Laeticia, Natacha Mrozek, Karl Stefic, Theis, Clément, Vidal, Magali, Sauvat, Léo, Benjamin Lefèvre, Delphine Martineau, Baronnet, Guillaume, Florence Ader, Agnès Didier, Perpoint, Thomas, Conrad, Anne, Chabert, Paul, Aurélie Martin, Pierre Chauvelot, Loubet, Paul, Mazet, Julien, Larcher, Romaric, Mathilde Devaux Jérôme Frey, Didier Laureillard, Woerlen, Amos, Remillon, Aline, Absensur-Vuillaume, Laure, Albert Trinh-Duc, Pauline Bouquet, Philippe Petua, Patrick Rispal, Aurore Perrot, Julien Carillo, Delavigne, Karen, Cougoul, Pierre, Dion, Jérémie, Mathieu Blot, Odile Rauzy, Sixt, Thibault, Moretto, Florian, Charles, Carole, Sophie Circosta, Lionel Piroth, Leger, Lydia, Arulananthan, Arulvani, Lascoux, Carine, Valérie, Pascaline, Yazdan Yazdanpanah, Leia Becam, Petrov-Sanchez, Ventzislava, Diallo, Alpha, Le Mestre, Soizic, Le Meut Isabelle Goderel, Guillaume, Chau, Frédéric, Soltana, Brahim, Chane Tang, Jessica, Guedj, Jérémie, Caille, Yvanie, Bruel, Timothée, Vrignaud, Lou-Léna, Porrot, Françoise, Staropoli, Isabelle, Planas, Delphine, Guivel-Benhassine, Florence, Puech, Julien, Prot, Matthieu, Munier, Sandie, Bolland, William Henry, Soulié, Cathia, Lusivika-Nzinga, Clovis, Meledge, Marie-Laure, Dorival, Céline, Molino, Diana, Péré, Hélène, Yordanov, Youri, Simon-Lorière, Etienne, Veyer, David, Carrat, Fabrice, Schwartz, Olivier, Marcelin, Anne-Geneviève, and Martin-Blondel, Guillaume

Published
2023
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10. Primaquine as a Candidate for HHV-8-Associated Primary Effusion Lymphoma and Kaposi’s Sarcoma Treatment

Author

Adélie Gothland, Valentin Leducq, Philippe Grange, Ousmane Faye, Laurianne Beauvais Remigereau, Sophie Sayon, Nathalie Désiré, Aude Jary, Emmanuel Laplantine, Almoustapha Issiaka Maiga, Nicolas Dupin, Anne-Geneviève Marcelin, Vincent Calvez, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Service de Virologie [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université de Bamako, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Bamako [Mali], Hôpital Gabriel Touré [Mali], and Gothland, Adélie

Subjects
[SDV.MHEP.HEM] Life Sciences [q-bio]/Human health and pathology/Hematology, Cancer Research, viruses, human herpesvirus 8, Kaposi’s sarcoma, primary effusion lymphoma, primaquine diphosphate, oxidative stress, apoptosis, Kaposi's sarcoma, virus diseases, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology, Oncology, [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, hemic and lymphatic diseases, [SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, RC254-282
Abstract

International audience; Human Herpesvirus 8 (HHV-8) is associated with three main severe orphan malignancies, Kaposi’s sarcoma (KS), multicentric Castleman’s disease (MCD), and primary effusion lymphoma (PEL), which present few therapeutic options. We identified the antimalarial primaquine diphosphate (PQ) as a promising therapeutic candidate for HHV-8-associated PEL and KS. Indeed, PQ strongly reduced cell viability through caspase-dependent apoptosis, specifically in HHV-8-infected PEL cells. Reactive oxygen species (ROS)- and endoplasmic reticulum (ER) stress-mediated apoptosis signaling pathways were found to be part of the in vitro cytotoxic effect of PQ. Moreover, PQ treatment had a clinically positive effect in a nonobese diabetic (NOD)/SCID xenograft PEL mouse model, showing a reduction in tumor growth and an improvement in survival. Finally, an exploratory proof-of-concept clinical trial in four patients harboring severe KS was conducted, with the main objectives to assess the efficacy, the safety, and the tolerability of PQ, and which demonstrated a positive efficacy on Kaposi’s sarcoma-related lesions and lymphedema.

Published
2022
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12. Cyclin-dependent kinase inhibitors p21 and p27 function as critical regulators of liver regeneration following 90% hepatectomy in the rat

Author

Laurence Lacaze, Adélie Gothland, Alice Deshayes, Jamila Faivre, Didier Samuel, and Nicolas Moniaux

Subjects
Major hepatectomy, p21, Hepatology, Kinase, Cell growth, business.industry, medicine.medical_treatment, Regeneration (biology), Liver failure, p27, Basic Study, Cell cycle, Liver regeneration, Andrology, 03 medical and health sciences, 0302 clinical medicine, 030220 oncology & carcinogenesis, Parenchyma, medicine, 030211 gastroenterology & hepatology, Hepatectomy, business, Post-hepatectomy liver failure, Cyclin
Abstract

Background Liver reduction is the main curative treatment for primary liver cancer, but its use remains limited as liver regeneration requires a minimum of 30% functional parenchyma. Aim To study the dynamics of the liver regeneration process and consequent behavior of cell cycle regulators in rats after extended hepatectomy (90%) and postoperative glucose infusions. Methods Post-hepatectomy liver failure was triggered in 84 Wistar rats by reducing their liver mass by 90%. The animals received a post-operative glucose infusion and were randomly assigned to two groups: One to investigate the survival rate and the other for biochemical analyses. Animals that underwent laparotomy or 70% hepatectomy were used as controls. Blood and liver samples were collected on postoperative days 1 to 7. Liver morphology, function, and regeneration were studied with histology, immunohistochemistry, and western blotting. Results Postoperative mortality after major resection reached 20% and 55% in the first 24 h and 48 h, respectively, with an overall total of 70% 7 d after surgery. No apparent signs of apoptotic cell death were detected in the extended hepatectomy rat livers, but hepatocytes displaying a clear cytoplasm and an accumulation of hyaline material testified to changes affecting their functional activities. Liver regeneration started properly, as early events initiating cell proliferation occurred within the first 3 h, and the G1 to S transition was detected in less than 12 h. However, a rise in p27 (Kip1) followed by p21 (Waf1/Cip1) cell cycle inhibitor levels led to a delayed S phase progression and mitosis. Overall, liver regeneration in rats with a 90% hepatectomy was delayed by 24 h and associated with a delayed onset and lower peak magnitude of hepatocellular deoxyribonucleic acid synthesis. Conclusion This work highlights the critical importance of the cyclin/cyclin-dependent kinase inhibitors of the Cip/Kip family in regulating the liver regeneration timeline following extended hepatectomy.

Published
2020
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13. New Kaposi’s sarcoma-associated herpesvirus variant in men who have sex with men associated with severe pathologies

Author

Adélie Gothland, Véronique Joly, Corinne Amiel, Nathalie Désiré, Christine Katlama, Héloïse Petit, Sidonie Lambert-Niclot, Aude Jary, Romain Palich, Jean-Philippe Spano, Valentin Leducq, Laure Surgers, Anne-Geneviève Marcelin, Diane Descamps, Vincent Calvez, and Ana Canestri

Subjects
Adult, Male, 0301 basic medicine, medicine.medical_specialty, Population, Context (language use), Genome, Viral, Disease, medicine.disease_cause, Asymptomatic, Men who have sex with men, Sexual and Gender Minorities, Viral Proteins, 03 medical and health sciences, 0302 clinical medicine, Epidemiology, Prevalence, medicine, Humans, Immunology and Allergy, Homosexuality, Male, Kaposi's sarcoma-associated herpesvirus, education, Sarcoma, Kaposi, Phylogeny, Retrospective Studies, education.field_of_study, business.industry, Genetic Variation, virus diseases, Middle Aged, medicine.disease, Virology, 3. Good health, 030104 developmental biology, Infectious Diseases, 030220 oncology & carcinogenesis, DNA, Viral, Herpesvirus 8, Human, France, Primary effusion lymphoma, medicine.symptom, business
Abstract

Background Kaposi sarcoma (KS)–associated herpesvirus (KSHV) subtype depends mostly on patient origin. The current study aimed to assess KSHV diversity in a population of men who have sex with men (MSM) living in France. Methods The study included 264 patients. In 65 MSM, including 57 human immunodeficiency virus (HIV)–infected men with KS, multicentric Castleman disease, or primary effusion lymphoma and 8 HIV-uninfected men receiving HIV preexposure prophylaxis (PrEP), we performed KSHV typing with K1 open reading frame Sanger and KSHV whole-genome sequencing. In 199 other patients, we performed real-time polymerase chain reaction screening for the new variant. Results We found that 51% of KSHV-strains were subtype C (85% C3), and 33% were subtype A. Four patients with severe KSHV disease (2 with visceral KS, 1 with multicentric Castleman disease, and 1 with primary effusion lymphoma) and 1 asymptomatic PrEP user had a new variant resembling the Ugandan subtype F, but with different K1 open reading frame and KSHV whole-genome sequences and a different epidemiological context (MSM vs African population). Its prevalence was 4.5% in Caucasian MSM, and it was absent in other epidemiological groups. Conclusions Subtype C predominated among MSM living in France. The new F variant was identified in Caucasian MSM and associated with severe KSHV disease, suggesting that subtype F could be split into F1 and F2 variants. Careful screening for this variant may be required in MSM, given the severe clinical presentation of associated diseases.

Published
2020
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14. Primaquine as a Candidate for HHV-8-Associated Primary Effusion Lymphoma and Kaposi’s Sarcoma Treatment

Author

Gothland, Adélie, primary, Leducq, Valentin, additional, Grange, Philippe, additional, Faye, Ousmane, additional, Beauvais Remigereau, Laurianne, additional, Sayon, Sophie, additional, Désiré, Nathalie, additional, Jary, Aude, additional, Laplantine, Emmanuel, additional, Maiga, Almoustapha Issiaka, additional, Dupin, Nicolas, additional, Marcelin, Anne-Geneviève, additional, and Calvez, Vincent, additional

Published
2022
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15. Kaposi’s Sarcoma-Associated Herpesvirus, the Etiological Agent of All Epidemiological Forms of Kaposi’s Sarcoma

Author

Aude Jary, Marianne Veyri, Adélie Gothland, Valentin Leducq, Vincent Calvez, Anne-Geneviève Marcelin, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Service d'Oncologie médicale [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), HAL-SU, Gestionnaire, Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)

Subjects
0303 health sciences, Cancer Research, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, viruses, Kaposi's sarcoma, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, virus diseases, Review, KSHV, oncogenic viral proteins, 3. Good health, 03 medical and health sciences, Kaposi’s sarcoma, 0302 clinical medicine, Oncology, oncogenic virus, 030220 oncology & carcinogenesis, LANA-1, RC254-282, HHV-8, latency, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030304 developmental biology
Abstract

International audience; Simple SummaryKaposi’s sarcoma-associated herpesvirus (KSHV) is one of the seven oncogenic viruses currently recognized by the International Agency for Research on Cancer. Its presence for Kaposi’s sarcoma development is essential and knowledge on the oncogenic process has increased since its discovery in 1994. However, some uncertainties remain to be clarified, in particular on the exact routes of transmission and disparities in KSHV seroprevalence and the prevalence of Kaposi’s sarcoma worldwide. Here, we summarized the current data on the KSHV viral particle’s structure, its genome, the replication, its seroprevalence, the viral diversity and the lytic and latent oncogenesis proteins involved in Kaposi’s sarcoma. Lastly, we reported the environmental, immunological and viral factors possibly associated with KSHV transmission that could also play a role in the development of Kaposi’s sarcoma.AbstractKaposi’s sarcoma-associated herpesvirus (KSHV), also called human herpesvirus 8 (HHV-8), is an oncogenic virus belonging to the Herpesviridae family. The viral particle is composed of a double-stranded DNA harboring 90 open reading frames, incorporated in an icosahedral capsid and enveloped. The viral cycle is divided in the following two states: a short lytic phase, and a latency phase that leads to a persistent infection in target cells and the expression of a small number of genes, including LANA-1, v-FLIP and v-cyclin. The seroprevalence and risk factors of infection differ around the world, and saliva seems to play a major role in viral transmission. KSHV is found in all epidemiological forms of Kaposi’s sarcoma including classic, endemic, iatrogenic, epidemic and non-epidemic forms. In a Kaposi’s sarcoma lesion, KSHV is mainly in a latent state; however, a small proportion of viral particles (

Published
2021
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16. Harnessing Redox Disruption to Treat Human Herpesvirus 8 (HHV-8) Related Malignancies.

Author

Gothland, Adélie, Jary, Aude, Grange, Philippe, Leducq, Valentin, Beauvais-Remigereau, Laurianne, Dupin, Nicolas, Marcelin, Anne-Geneviève, and Calvez, Vincent

Subjects
KAPOSI'S sarcoma, OXIDATION-reduction reaction, REACTIVE oxygen species, CELL growth, ONCOGENIC viruses, PROGRAMMED cell death 1 receptors, CELL death
Abstract

Reprogrammed metabolism is regarded as a hallmark of cancer and offers a selective advantage to tumor cells during carcinogenesis. The redox equilibrium is necessary for growth, spread and the antioxidant pathways are boosted following Reactive Oxygen Species (ROS) production to prevent cell damage in tumor cells. Human herpesvirus 8 (HHV-8), the etiologic agent of Kaposi sarcoma KS and primary effusion lymphoma (PEL), is an oncogenic virus that disrupts cell survival-related molecular signaling pathways leading to immune host evasion, cells growths, angiogenesis and inflammatory tumor-environment. We recently reported that primaquine diphosphate causes cell death by apoptosis in HHV-8 infected PEL cell lines in vivo and exhibits therapeutic anti-tumor activity in mice models and advanced KS. Our findings also suggest that the primaquine-induced apoptosis in PEL cells is mostly influenced by ROS production and targeting the redox balance could be a new approach to treat HHV-8 related diseases. In this review, we summarized the knowledge about the influence of ROS in cancer development; more specifically, the proof of evidence from our work and from the literature that redox pathways are important for the development of HHV-8 pathologies. [ABSTRACT FROM AUTHOR]

Published
2023
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17. Primaquine as an innovative and targeted therapeutic strategy for HHV-8-associated diseases

Author

Gothland, Adélie, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Sorbonne Université, Vincent Calvez, and STAR, ABES

Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Maladie de Kaposi, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Apoptose, Stress oxydatif, Kaposi's sarcoma, Primary effusion lymphoma, Primaquine diphosphate, Stress du réticulum endoplasmique, Herpèsvirus humain 8, Oxidative stress, Lymphome primitif des séreuses, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Endoplasmic reticulum stress, Human herpesvirus 8, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

The Human Herpesvirus 8 (HHV-8) is one of seven recognized human cancer causing viruses. HHV-8 is a principal causative agent of several human cancers including Kaposi’s sarcoma (KS), multicentric Castleman’s disease (MCD) and primary effusion lymphoma (PEL), which represent important and difficult to treat clinical problem, with very few therapeutic options. In the present in vitro study, we demonstrated the specific cytotoxic and pro-apoptotic effect of the antimalarial primaquine disphosphate in HHV-8-infected PEL cells, without any toxicity on different uninfected cells. We also found that primaquine-induced oxidative stress and pro-apoptotic UPR activated by excessive reticulum endoplasmic stress, to be part of the pro-apoptotic mechanisms of action of primaquine in PEL cells. Finally, PQ treatment had a clinical positive effect on tumor growth in a in vivo NOD/SCID xenograft PEL mouse model, as well as in a pilot clinical study in human harboring very severe KS. Importantly, primaquine was well tolerated and none adverse event and overt toxic effects were observed in both primaquine-treated mice and patients. The combination of preclinical and clinical observations and results from our analysis thereby raising the possibility that primaquine may be used as a promising targeted agent in treatment of HHV-8-associated cancers., L’Herpèsvirus Humain 8 (HHV-8) est l’un des sept virus humains reconnus à caractère oncogénique. Les principales pathologies tumorales associées au virus HHV-8, à savoir la maladie de Kaposi (KS), le lymphome primitif des séreuses (PEL) et la maladie de Castleman multicentrique (MCD), représentent encore à ce jour un véritable défi clinique, étant donné le peu d’options thérapeutiques disponibles et efficaces pour y faire face. Au cours de nos études in vitro nous avons pu démontrer l’effet cytotoxique et pro-apoptotique de l’antipaludéen primaquine diphosphate, spécifique aux lignées cellulaires de PEL infectées par l’HHV-8. Nous avons également pu mettre en évidence que l’induction d’un stress oxydatif, ainsi que des voies pro-apoptotiques de l’UPR liées à un stress sévère du réticulum endoplasmique, prenait part aux mécanismes d’actions mis en œuvre dans l’effet pro-apoptotique de la primaquine. Enfin, des études in vivo ont permis de montrer une efficacité anti-tumorale très encourageante de la primaquine, d’une part chez un modèle de souris PEL et d’autre part, au cours d’une étude pilote chez des patients présentant une forme sévère du KS. Aucuns effets secondaires manifestes n’ont été observés chez les souris et les patients traités à la primaquine. Ces études précliniques et cliniques permettent donc de considérer la primaquine diphosphate comme un nouveau candidat très prometteur pour le traitement des cancers associés à l’HHV-8.

Published
2021

19. La Primaquine, une thérapie innovante et ciblée pour les pathologies associées à l’infection par l’Herpèsvirus humain 8

Author

Gothland, Adélie and STAR, ABES

Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Maladie de Kaposi, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Apoptose, Stress oxydatif, Kaposi's sarcoma, Primary effusion lymphoma, Primaquine diphosphate, Stress du réticulum endoplasmique, Herpèsvirus humain 8, Oxidative stress, Lymphome primitif des séreuses, Endoplasmic reticulum stress, Human herpesvirus 8
Abstract

The Human Herpesvirus 8 (HHV-8) is one of seven recognized human cancer causing viruses. HHV-8 is a principal causative agent of several human cancers including Kaposi’s sarcoma (KS), multicentric Castleman’s disease (MCD) and primary effusion lymphoma (PEL), which represent important and difficult to treat clinical problem, with very few therapeutic options. In the present in vitro study, we demonstrated the specific cytotoxic and pro-apoptotic effect of the antimalarial primaquine disphosphate in HHV-8-infected PEL cells, without any toxicity on different uninfected cells. We also found that primaquine-induced oxidative stress and pro-apoptotic UPR activated by excessive reticulum endoplasmic stress, to be part of the pro-apoptotic mechanisms of action of primaquine in PEL cells. Finally, PQ treatment had a clinical positive effect on tumor growth in a in vivo NOD/SCID xenograft PEL mouse model, as well as in a pilot clinical study in human harboring very severe KS. Importantly, primaquine was well tolerated and none adverse event and overt toxic effects were observed in both primaquine-treated mice and patients. The combination of preclinical and clinical observations and results from our analysis thereby raising the possibility that primaquine may be used as a promising targeted agent in treatment of HHV-8-associated cancers., L’Herpèsvirus Humain 8 (HHV-8) est l’un des sept virus humains reconnus à caractère oncogénique. Les principales pathologies tumorales associées au virus HHV-8, à savoir la maladie de Kaposi (KS), le lymphome primitif des séreuses (PEL) et la maladie de Castleman multicentrique (MCD), représentent encore à ce jour un véritable défi clinique, étant donné le peu d’options thérapeutiques disponibles et efficaces pour y faire face. Au cours de nos études in vitro nous avons pu démontrer l’effet cytotoxique et pro-apoptotique de l’antipaludéen primaquine diphosphate, spécifique aux lignées cellulaires de PEL infectées par l’HHV-8. Nous avons également pu mettre en évidence que l’induction d’un stress oxydatif, ainsi que des voies pro-apoptotiques de l’UPR liées à un stress sévère du réticulum endoplasmique, prenait part aux mécanismes d’actions mis en œuvre dans l’effet pro-apoptotique de la primaquine. Enfin, des études in vivo ont permis de montrer une efficacité anti-tumorale très encourageante de la primaquine, d’une part chez un modèle de souris PEL et d’autre part, au cours d’une étude pilote chez des patients présentant une forme sévère du KS. Aucuns effets secondaires manifestes n’ont été observés chez les souris et les patients traités à la primaquine. Ces études précliniques et cliniques permettent donc de considérer la primaquine diphosphate comme un nouveau candidat très prometteur pour le traitement des cancers associés à l’HHV-8.

Published
2021

20. Rapid decline of neutralizing antibodies against SARS-CoV-2 among infected healthcare workers

Author

Marot, Stéphane, Malet, Isabelle, Leducq, Valentin, Zafilaza, Karen, Sterlin, Delphine, Planas, Delphine, Gothland, Adélie, Jary, Aude, Dorgham, Karim, Bruel, Timothée, Attali, Valérie, Baresse, Isabelle, Beurton, Alexandra, Boddaert, Jacques, Bourmaleau, Julie, Catala, Martin, Demoule, Alexandre, Dunoyer, Violaine, Esteban-Amarilla, Cristina, Hausfater, Pierre, Haziot, Noémie, Ip, Queyras, Kubis, Nathalie, Lhoest, Laurence, Lubetzki, Catherine, Marion, Fabienne, Morawiec, Elise, Mourtada, Leila, Orcel, Brigitte, Morelot-Panzini, Capucine, Raux, Mathieu, Reinhard, Christophe, Riquier, Claire, Roubertier, Xavier, Weiss, Nicolas, Zalc, Bernard, Burrel, Sonia, Boutolleau, David, Schwartz, Olivier, Gorochov, Guy, Calvez, Vincent, Marcelin, Anne-Geneviève, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology, Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris], Centre d'Immunologie et de Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Vaccine Research Institute (VRI), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Virus et Immunité - Virus and immunity, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), The AGM laboratory is funded by Agence Nationale de Recherches sur le SIDA et les hépatites virales (AC43, Medical Virology), the SARS-CoV-2 Program of the Faculty of Medicine of Sorbonne University, the Agence Nationale de la Recherche. The GG laboratory is supported by the Fondation de France, 'Tous unis contre le virus' framework Alliance (Fondation de France, AP-HP, Institut Pasteur) in collaboration with the Agence Nationale de la Recherche (ANR Flash COVID19 program) and by the SARS-CoV-2 Program of the Faculty of Medicine of Sorbonne University ICOViD programs. The OS laboratory is funded by Institut Pasteur, the ANRS, the Vaccine Research Institute (ANR-10-LABX-77), Labex IBEID (ANR-10-LABX-62-IBEID), 'TIMTAMDEN' ANR-14-CE14-0029, 'CHIKV-Viro-Immuno' ANR-14-CE14-0015-01, the Gilead HIV cure program, ANR/FRM Flash Covid PROTEO-SARS-CoV-2 and IDISCOVR, and Fondation pour la Recherche Médicale. We thank Nathalie Aulner and the UtechS Photonic BioImaging (UPBI) core facility (Institut Pasteur), a member of the France BioImaging network, for image acquisition and analysis., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-LABX-0077,VRI,Initiative for the creation of a Vaccine Research Institute(2010), ANR-14-CE14-0029,TIMTAMDEN,Rôle des récepteurs TIM et TAM dans l'infection des cellules cibles par le virus de la dengue(2014), ANR-14-CE14-0015,CHIKV-Viro-Immuno,Multiplication et Relation avec l'hôte du virus Chikungunya(2014), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Vaccine Research Institute [Créteil, France] (VRI), Virus et Immunité - Virus and immunity (CNRS-UMR3569), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), HAL-SU, Gestionnaire, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Laboratoires d'excellence - Initiative for the creation of a Vaccine Research Institute - - VRI2010 - ANR-10-LABX-0077 - LABX - VALID, Appel à projets générique - Rôle des récepteurs TIM et TAM dans l'infection des cellules cibles par le virus de la dengue - - TIMTAMDEN2014 - ANR-14-CE14-0029 - Appel à projets générique - VALID, and Appel à projets générique - Multiplication et Relation avec l'hôte du virus Chikungunya - - CHIKV-Viro-Immuno2014 - ANR-14-CE14-0015 - Appel à projets générique - VALID

Subjects
0301 basic medicine, Immunoglobulin A, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Science, General Physics and Astronomy, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Article, General Biochemistry, Genetics and Molecular Biology, Persistence (computer science), 03 medical and health sciences, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Medicine, Infection control, 030212 general & internal medicine, [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity, [SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, Multidisciplinary, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, biology, business.industry, SARS-CoV-2, General Chemistry, biochemical phenomena, metabolism, and nutrition, 3. Good health, Vaccination, Titer, 030104 developmental biology, Viral infection, Immunology, biology.protein, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, sense organs, Antibody, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

There are only few data concerning persistence of neutralizing antibodies (NAbs) among SARS-CoV-2-infected healthcare workers (HCW). These individuals are particularly exposed to SARS-CoV-2 infection and at potential risk of reinfection. We followed 26 HCW with mild COVID-19 three weeks (D21), two months (M2) and three months (M3) after the onset of symptoms. All the HCW had anti-receptor binding domain (RBD) IgA at D21, decreasing to 38.5% at M3 (p, The humoral immune response to SARS-CoV-2 infection is not yet fully understood. Here, Marot et al. monitor the longitudinal profile and neutralizing activity of IgG, IgA, and IgM among 26 healthcare workers and provide evidence for a short-lasting humoral immune protection due to a decrease of neutralizing antibody titers within 3 months.

Published
2021
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21. Poppers, by Inducing HHV-8 Virion Production, Can Act as a Promoter for HHV-8 Transmission in Men Who Have Sex With Men

Author

Jean-Philippe Spano, Anne-Geneviève Marcelin, Valentin Leducq, Jean-Claude Alvarez, Vincent Calvez, Aude Jary, Sébastien Imbert, Islam Amine Larabi, Christine Katlama, Gentiane Monsel, Anne Simon, Romain Palich, Luminita Schneider, Adélie Gothland, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Service de Parasitologie - Mycologie [CHU Pitié-Salpétrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Paris 1 Panthéon-Sorbonne (UP1), Hôpital Raymond Poincaré [AP-HP], Gestionnaire, HAL Sorbonne Université 5, Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de parasitologie - mycologie [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], and Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)

Subjects
viruses, chemsex, Population, poppers, HHV-8 production, Men who have sex with men, 03 medical and health sciences, 0302 clinical medicine, In vivo, medicine, MSM, education, HHV-8, 030304 developmental biology, 0303 health sciences, education.field_of_study, Transmission (medicine), business.industry, Brief Report, virus diseases, biochemical phenomena, metabolism, and nutrition, Virology, humanities, 3. Good health, Infectious Diseases, AcademicSubjects/MED00290, Oncology, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, 030220 oncology & carcinogenesis, HIV PrEP, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, business, Amyl nitrite, Human herpesvirus, medicine.drug
Abstract

Environmental factors were reported to increase the risk of human herpesvirus 8 (HHV-8) transmission. In a population of men who have sex with men (MSM), we found evidence that chemsex was associated with human herpesvirus 8 seropositivity in vivo and that poppers induced HHV-8 virion production in vitro. Our finding may explain the higher HHV-8 transmission in MSM.

Published
2020
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22. New Kaposi's sarcoma-associated herpesvirus variant in men who have sex with men 3 associated with severe pathologies Running title: New KSHV variant identified in MSM

Author

Jary, Aude, Leducq, Valentin, Désiré, Nathalie, Petit, Héloïse, Palich, Romain, Joly, Véronique, Canestri, Ana, Gothland, Adélie, Lambert-Niclot, Sidonie, Surgers, Laure, Amiel, Corinne, Descamps, Diane, Spano, Jean-Philippe, Katlama, Christine, Calvez, Vincent, Marcelin, Anne-Geneviève, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Services de Maladies Infectieuses et Tropicales [CHU Bichat], AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des maladies infectieuses et tropicales [CHU Tenon], CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Services des Maladies Infectieuses et Tropicales [CHU Saint-Antoine], CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de virologie [Hôpital Tenon], Service de Virologie [CHU Bichat], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d'Oncologie médicale [CHU Pitié-Salpêtrière], and CHU Pitié-Salpêtrière [AP-HP]

Subjects
ORF-K1, whole-genome sequencing, viruses, phylogenetic analysis, virus diseases, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, KSHV, MSM, new variant
Abstract

International audience; BackgroundKaposi sarcoma (KS)–associated herpesvirus (KSHV) subtype depends mostly on patient origin. The current study aimed to assess KSHV diversity in a population of men who have sex with men (MSM) living in France.MethodsThe study included 264 patients. In 65 MSM, including 57 human immunodeficiency virus (HIV)–infected men with KS, multicentric Castleman disease, or primary effusion lymphoma and 8 HIV-uninfected men receiving HIV preexposure prophylaxis (PrEP), we performed KSHV typing with K1 open reading frame Sanger and KSHV whole-genome sequencing. In 199 other patients, we performed real-time polymerase chain reaction screening for the new variant.ResultsWe found that 51% of KSHV-strains were subtype C (85% C3), and 33% were subtype A. Four patients with severe KSHV disease (2 with visceral KS, 1 with multicentric Castleman disease, and 1 with primary effusion lymphoma) and 1 asymptomatic PrEP user had a new variant resembling the Ugandan subtype F, but with different K1 open reading frame and KSHV whole-genome sequences and a different epidemiological context (MSM vs African population). Its prevalence was 4.5% in Caucasian MSM, and it was absent in other epidemiological groups.ConclusionsSubtype C predominated among MSM living in France. The new F variant was identified in Caucasian MSM and associated with severe KSHV disease, suggesting that subtype F could be split into F1 and F2 variants. Careful screening for this variant may be required in MSM, given the severe clinical presentation of associated diseases.

Published
2020
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23. Bridging β-Cyclodextrin Prevents Self-Inclusion, Promotes Supramolecular Polymerization, and Promotes Cooperative Interaction with Nucleic Acids

Author

Dmitri Colesnic, Vincent Calvez, Mickaël Ménand, Laurent Bouteiller, Matthieu Sollogoub, Pierre Evenou, Sergii Rudiuk, Anne-Geneviève Marcelin, Adélie Gothland, Gaëlle Pembouong, Julien Rossignol, Renaud Barbeyron, Damien Baigl, Institut de Recherche en Communications et en Cybernétique de Nantes (IRCCyN), Mines Nantes (Mines Nantes)-École Centrale de Nantes (ECN)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-PRES Université Nantes Angers Le Mans (UNAM)-Centre National de la Recherche Scientifique (CNRS), Université Sorbonne Paris Cité (USPC), Chimie des polymères (LCP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Parisien de Chimie Moléculaire (IPCM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut Parisien de Chimie Moléculaire (IPCM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640) (PASTEUR), Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Numerical Medicine (NUMED), Unité de Mathématiques Pures et Appliquées (UMPA-ENSL), École normale supérieure de Lyon (ENS de Lyon)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Centre National de la Recherche Scientifique (CNRS)-Inria Grenoble - Rhône-Alpes, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Chimie Moléculaire de Paris Centre (FR 2769), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Chimie du CNRS (INC)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Inria Grenoble - Rhône-Alpes, Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Inria Grenoble - Rhône-Alpes, and bouteiller, laurent

Subjects
genetic structures, Supramolecular chemistry, Substituent, Transfection, 010402 general chemistry, 01 natural sciences, Catalysis, Polymerization, Small Molecule Libraries, chemistry.chemical_compound, [CHIM] Chemical Sciences, [CHIM]Chemical Sciences, RNA, Small Interfering, Luciferases, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, Cyclodextrin, 010405 organic chemistry, beta-Cyclodextrins, technology, industry, and agriculture, DNA, General Chemistry, General Medicine, Combinatorial chemistry, 3. Good health, 0104 chemical sciences, Supramolecular polymers, Microscopy, Fluorescence, chemistry, Nucleic acid, lipids (amino acids, peptides, and proteins), Hydrophobic and Hydrophilic Interactions, Linker
Abstract

A bridge to assemble: Cyclodextrins bridged with an ammonium linker bearing a hydrophobic substituent can efficiently form supramolecular polymers and avoid the competing self-inclusion and head-to-head processes. Furthermore, the self-assembling cyclodextrin derivative interacts in a highly cooperative manner with DNA, as demonstrated by compaction experiments. It also interacts cooperatively with siRNA and allows its transfection.

Published
2018
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24. Poppers, by Inducing HHV-8 Virion Production, Can Act as a Promoter for HHV-8 Transmission in Men Who Have Sex With Men

Author

Jary, Aude, primary, Gothland, Adélie, additional, Palich, Romain, additional, Leducq, Valentin, additional, Monsel, Gentiane, additional, Imbert, Sébastien, additional, Schneider, Luminita, additional, Simon, Anne, additional, Spano, Jean-Philippe, additional, Katlama, Christine, additional, Larabi, Islam Amine, additional, Alvarez, Jean-Claude, additional, Calvez, Vincent, additional, and Marcelin, Anne-Geneviève, additional

Published
2021
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27. New Kaposi’s sarcoma-associated herpesvirus variant in men who have sex with men associated with severe pathologies

Author

Jary, Aude, primary, Leducq, Valentin, additional, Desire, Nathalie, additional, Petit, Héloïse, additional, Palich, Romain, additional, Joly, Véronique, additional, Canestri, Ana, additional, Gothland, Adélie, additional, Lambert-Niclot, Sidonie, additional, Surgers, Laure, additional, Amiel, Corinne, additional, Descamps, Diane, additional, Spano, Jean-Philippe, additional, Katlama, Christine, additional, Calvez, Vincent, additional, and Marcelin, Anne-Geneviève, additional

Published
2020
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28. Usefulness of Kaposi's Sarcoma-Associated Herpesvirus (KSHV) DNA Viral Load in Whole Blood for Diagnosis and Monitoring of KSHV-Associated Diseases

Author

Diane Descamps, Aude Jary, Vincent Calvez, Ana Canestri, Elyanne Gault, Jean-Philippe Spano, Sidonie Lambert-Niclot, Audrey Mirand, Astrid Vabret, Marc-Antoine Valantin, Valentin Leducq, Adélie Gothland, Anne-Geneviève Marcelin, Nicolas Dupin, Romain Palich, Corinne Amiel, Véronique Joly, Christine Katlama, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), CHU Tenon [AP-HP], Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Numerical Medicine (NUMED), Unité de Mathématiques Pures et Appliquées (UMPA-ENSL), École normale supérieure de Lyon (ENS de Lyon)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Centre National de la Recherche Scientifique (CNRS)-Inria Grenoble - Rhône-Alpes, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire d'Informatique Médicale et de BIOinformatique (LIM&BIO), Université Paris 13 (UP13), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des maladies infectieuses et tropicales [CHU Tenon], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service des maladies infectieuses et tropicales [CHU Pitié-Salpêtrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Inria Grenoble - Rhône-Alpes

Subjects
0301 basic medicine, Microbiology (medical), Adult, Male, 030106 microbiology, HIV Infections, medicine.disease_cause, Real-Time Polymerase Chain Reaction, 03 medical and health sciences, chemistry.chemical_compound, Herpesviridae Infections, Young Adult, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Lymphoma, Primary Effusion, medicine, Humans, Kaposi's sarcoma-associated herpesvirus, Sarcoma, Kaposi, Letter to the Editor, ComputingMilieux_MISCELLANEOUS, Whole blood, Aged, Retrospective Studies, Aged, 80 and over, business.industry, Castleman disease, Castleman Disease, Middle Aged, Viral Load, medicine.disease, Virology, 3. Good health, Real-time polymerase chain reaction, chemistry, DNA, Viral, Herpesvirus 8, Human, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Biomarker (medicine), Female, business, Viral load, DNA
Abstract

International audience

Published
2018
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29. Usefulness of Kaposi's Sarcoma-Associated Herpesvirus (KSHV) DNA Viral Load in Whole Blood for Diagnosis and Monitoring of KSHV-Associated Diseases

Author

Jary, Aude, primary, Leducq, Valentin, additional, Palich, Romain, additional, Gothland, Adélie, additional, Descamps, Diane, additional, Joly, Véronique, additional, Lambert-Niclot, Sidonie, additional, Amiel, Corinne, additional, Canestri, Ana, additional, Mirand, Audrey, additional, Gault, Elyanne, additional, Vabret, Astrid, additional, Valantin, Marc-Antoine, additional, Katlama, Christine, additional, Calvez, Vincent, additional, Dupin, Nicolas, additional, Spano, Jean-Philippe, additional, and Marcelin, Anne-Geneviève, additional

Published
2018
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30. Bridging β-Cyclodextrin Prevents Self-Inclusion, Promotes Supramolecular Polymerization, and Promotes Cooperative Interaction with Nucleic Acids

Author

Evenou, Pierre, primary, Rossignol, Julien, additional, Pembouong, Gaëlle, additional, Gothland, Adélie, additional, Colesnic, Dmitri, additional, Barbeyron, Renaud, additional, Rudiuk, Sergii, additional, Marcelin, Anne-Geneviève, additional, Ménand, Mickaël, additional, Baigl, Damien, additional, Calvez, Vincent, additional, Bouteiller, Laurent, additional, and Sollogoub, Matthieu, additional

Published
2018
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31. Highly polymorphic microsatellite markers in two species, the invasive shore crabs Hemigraspus sanguineus and Hemigraspus takanoi (Decapoda, Varunidae)

Author

Céline Poux, Nicolas Spilmont, Moâna Gothland, Jean-François Arnaud, Anne-Catherine Holl, Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 (Evo-Eco-Paléo), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG), Centre National de la Recherche Scientifique (CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut national des sciences de l'Univers (INSU - CNRS), Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 (Evo-Eco-Paléo (EEP)), and Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Nord])

Subjects
education.field_of_study, Genetic diversity, [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], biology, Ecology, Decapoda, Hemigrapsus sanguineus, Population, Hemigrapsus takanoi, Zoology, biology.organism_classification, Hemigrapsus, Varunidae, Genetics, Polymorphic Microsatellite Marker, 14. Life underwater, education, Ecology, Evolution, Behavior and Systematics, ComputingMilieux_MISCELLANEOUS
Abstract

Varunid crabs Hemigrapsus sanguineus and Hemigrapsus takanoi are species native from intertidal coastal areas in North-western Pacific and have become invasive along the Atlantic European coasts. To gain insights into population genetic features, we developed and characterized 16 (H. sanguineus) and 11 (H. takanoi) novel polymorphic microsatellite loci from next-generation sequencing. The number of alleles ranged from 2 to 20 for H. sanguineus, and from 8 to 21 for H. takanoi. Expected heterozygosity ranged from 0.470 to 0.947 and from 0.313 to 0.781, with mean multilocus F is estimates suggesting rapid turnover of populations. Overall, these microsatellite markers showed very high levels of polymorphism that will facilitate population genetic studies devoted to track down the most likely sources of introduction.

Published
2015
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32. Biological traits explain the distribution and colonisation ability of the invasive shore crab Hemigrapsus takanoi

Author

J. Ovaert, A. Tous Rius, Jean-Philippe Pezy, S. Jobert, Jean-Claude Dauvin, Moana Gothland, F. Dufossé, Lionel Denis, Nicolas Spilmont, Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 ( LOG ), Université du Littoral Côte d'Opale-Université de Lille-Centre National de la Recherche Scientifique ( CNRS ), Morphodynamique Continentale et Côtière ( M2C ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ), Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG), Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Nord]), Morphodynamique Continentale et Côtière (M2C), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), and Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Shore, geography, education.field_of_study, geography.geographical_feature_category, [ SDU.STU.OC ] Sciences of the Universe [physics]/Earth Sciences/Oceanography, Ecology, 010604 marine biology & hydrobiology, Population, Hemigrapsus takanoi, Introduced species, Marine invertebrates, Aquatic Science, Biology, Oceanography, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, Invasive species, Colonisation, Biological dispersal, 14. Life underwater, education, [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography
Abstract

International audience; Comprehending marine invasions requires a better knowledge of the biological traits of invasive species, and the future spread of invasive species may be predicted through comprehensive overviews of their distribution. This study thus presents the current distribution of a non-indigenous species, the Asian shore crab Hemigrapsus takanoi, as well as the species population characteristics (size distribution and cohorts), based on a five-year survey (2008e2012) along the French coast of the English Channel. Two large populations were found near harbours: one on the Opal Coast (where density reached 61 ! 22 ind.m"2, mean ! s.d., in Dunkirk harbour) and one on the Calvados coast (density up to 26 ! 6 ind.m"2, mean ! s.d, in Honfleur harbour). H. takanoi exhibited a short life cycle, a rapid growth, an early sexual maturity and a high adult mortality. These features, combined with previously described high fecundity and high dispersal ability, endow this species with an 'r-selected strategy'. This strategy, which usually characterises species with a high colonisation ability, would explain the success of H. takanoi for colonising the French coast of the Channel. However, the species was found only in harbours and their vicinity; H. takanoi thus exhibited a discontinuous distribution along the 700 km of coastline. These results are discussed regarding sediment preference and potential introduction vectors. Hemigrapsus takanoi is now considered as established on the French coast and further studies are needed to evaluate the consequences of its introduction on the structure and functioning of the impacted shores

Published
2014
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33. Additional records and distribution (2011-2012) of Hemigrapsus sanguineus (De Haan, 1835) along the French coast of the English Channel

Author

Sandra Jobert, Julien Ovaert, Lionel Denis, Jean-Philippe Pezy, Nicolas Spilmont, Jean-Claude Dauvin, Moana Gothland, Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 ( LOG ), Université du Littoral Côte d'Opale-Université de Lille-Centre National de la Recherche Scientifique ( CNRS ), Morphodynamique Continentale et Côtière ( M2C ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ), Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG), Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Nord]), Morphodynamique Continentale et Côtière (M2C), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), and Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Hemigrapsus sanguineus, [SDE.MCG]Environmental Sciences/Global Changes, Population, invasive marine species, Introduced species, Ecological succession, Management, Monitoring, Policy and Law, 010603 evolutionary biology, 01 natural sciences, Population density, [ SDU.ENVI ] Sciences of the Universe [physics]/Continental interfaces, environment, 14. Life underwater, education, [SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces, environment, Ecology, Evolution, Behavior and Systematics, Shore, geography, education.field_of_study, English Channel, geography.geographical_feature_category, Ecology, biology, 010604 marine biology & hydrobiology, Aquatic animal, Marine invertebrates, biology.organism_classification, [ SDE.MCG ] Environmental Sciences/Global Changes, Asian shore crab
Abstract

International audience; The invasion process can be described as a succession of stages initiated by the transport of organisms from their native range to a new area where they persist, proliferate and spread. It is important to monitor the demographic development of invaders for management purposes. This study focuses on the different stages of population development during the invasion process and underlines the importance of understanding and monitoring the 'persistence phase'. The distribution of Hemigrapsus sanguineus (Asian shore crab) in the English Channel, along the French coast, was first undertaken in 2008. In 2010, 35 sites were surveyed and it appeared that the abundance of this species had already established a 2-5 fold increase since 2008. The present study presents the geographical distribution of H. sanguineus in 2011 and 2012 which includes a further 39 sites (72 sampling stations in 2012). All populations observed during previous years persisted in 2011 and 2012. In 2012, H. sanguineus was detected at 61 sites; 36 intensely colonised (including 3 newly colonised sites compared to 2011); 22 had trace numbers and 3 sites had 'proven presence'. In addition to males with carapaces up to 39 mm width (CW), abundances increased by a factor of 2 since 2010, which testifies for the naturalized status of the species along the French coast of the English Channel. Since 2008, La Hougue proved to be the most abundantly colonised site along the French coast. By 2011 it had an average density of 101±19 ind.m-2, with an abundance of 258±54 individuals (under 30 boulders). Populations were subsequently halved in 2012. Increasing densities and abundances recorded between 2008 and 2011 at la Hougue suggest that H. sanguineus had reached the 'expansion phase', but the dynamics of H. sanguineus populations at the most colonised sites (12 sampling sites with abundance >200 individuals under 30 boulders), suggest that maximum values had already peaked and that the 'persistence phase' was probably reached. The implementation of pluri-annual surveys seems of prime importance to correctly evaluate population dynamics of alien species.

Published
2013
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34. Bridging β‐Cyclodextrin Prevents Self‐Inclusion, Promotes Supramolecular Polymerization, and Promotes Cooperative Interaction with Nucleic Acids.

Author

Rossignol, Julien, Pembouong, Gaëlle, Colesnic, Dmitri, Barbeyron, Renaud, Ménand, Mickaël, Bouteiller, Laurent, Sollogoub, Matthieu, Evenou, Pierre, Gothland, Adélie, Marcelin, Anne‐Geneviève, Calvez, Vincent, Rudiuk, Sergii, and Baigl, Damien

Subjects
SUPRAMOLECULAR chemistry, POLYMERIZATION, NUCLEIC acids, CYCLODEXTRINS, AMMONIUM
Published
2018
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35. Etude des variations intra-thalles, saisonnières et géographiques des acides gras de trois phéophycées (P. canaliculata, F. spiralis et F. serratus) pour la mise en place d'outils trophiques

Author

Gothland, Moana, Spilmont, Nicolas, Mortillaro, J M, Méziane, T., Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG), Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Nord]), François G Schmitt, and Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography
Abstract

Les résultats obtenus lors des diverses analyses menées dans cette étude sur les compositions en acides gras (AGs) de trois phéophycées, mettent en évidence des variations de proportions en AGs en fonction des saisons et de la partie du thalle étudiée. Ceci porte ainsi à réfléchir sur le choix de la méthodologie à adopter quant à l'étude des profils d'acides gras et à l'identification de marqueurs macroalgaux adaptés.

Published
2012

37. SARS-CoV-2 VARIANTS-DEPENDENT INFLAMMASOME ACTIVATION.

Author

Teyssou, Elisa, Marot, Stephane, Gothland, Adelie, Malet, Isabelle, Zafilaza, Karen, Leducq, Valentin, Cocherie, Theophile, Soulié, Cathia, Marcelin, Anne-Geneviève, and Calvez, Vincent

Published
2023

39. Author Correction: Rapid decline of neutralizing antibodies against SARS-CoV-2 among infected healthcare workers.

Author

Marot, Stéphane, Malet, Isabelle, Leducq, Valentin, Zafilaza, Karen, Sterlin, Delphine, Planas, Delphine, Gothland, Adélie, Jary, Aude, Dorgham, Karim, Bruel, Timothée, the Sorbonne Université SARS-CoV-2 Neutralizing Antibodies Study Group, Attali, Valérie, Baresse, Isabelle, Beurton, Alexandra, Boddaert, Jacques, Bourmaleau, Julie, Catala, Martin, Demoule, Alexandre, Dunoyer, Violaine, and Esteban-Amarilla, Cristina

Subjects
MEDICAL personnel, SARS-CoV-2, IMMUNOGLOBULINS, AUTHORS
Abstract

This error has been corrected in both the PDF and HTML versions of the Article. The original version of this Article contained errors in the figure legends for Figs. [Extracted from the article]

Published
2021
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41. Rapid decline of neutralizing antibodies against SARS-CoV-2 among infected healthcare workers.

Author

Marot S, Malet I, Leducq V, Zafilaza K, Sterlin D, Planas D, Gothland A, Jary A, Dorgham K, Bruel T, Burrel S, Boutolleau D, Schwartz O, Gorochov G, Calvez V, and Marcelin AG

Subjects
Adult, Binding Sites immunology, COVID-19 virology, Cell Line, Tumor, Female, Humans, Immunoglobulin A immunology, Male, Middle Aged, Protein Binding, Receptors, Virus metabolism, SARS-CoV-2 physiology, Spike Glycoprotein, Coronavirus immunology, Spike Glycoprotein, Coronavirus metabolism, Time Factors, Antibodies, Neutralizing immunology, Antibodies, Viral immunology, COVID-19 immunology, Health Personnel statistics & numerical data, SARS-CoV-2 immunology
Abstract

There are only few data concerning persistence of neutralizing antibodies (NAbs) among SARS-CoV-2-infected healthcare workers (HCW). These individuals are particularly exposed to SARS-CoV-2 infection and at potential risk of reinfection. We followed 26 HCW with mild COVID-19 three weeks (D21), two months (M2) and three months (M3) after the onset of symptoms. All the HCW had anti-receptor binding domain (RBD) IgA at D21, decreasing to 38.5% at M3 (p < 0.0001). Concomitantly a significant decrease in NAb titers was observed between D21 and M2 (p = 0.03) and between D21 and M3 (p < 0.0001). Here, we report that SARS-CoV-2 can elicit a NAb response correlated with anti-RBD antibody levels. However, this neutralizing activity declines, and may even be lost, in association with a decrease in systemic IgA antibody levels, from two months after disease onset. This short-lasting humoral protection supports strong recommendations to maintain infection prevention and control measures in HCW, and suggests that periodic boosts of SARS-CoV-2 vaccination may be required.

Published
2021
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