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190 results on '"Gosalvez, J."'

1. Dynamics of sperm DNA fragmentation in domestic animals: II. The stallion

Author

Lopez-Fernandez, C., Crespo Castejón, Francisco, Arroyo, F., Fernandez, J. L., Arana Montes, María Del Pilar De, Johnston, S. D., Gosalvez, J., Lopez-Fernandez, C., Crespo Castejón, Francisco, Arroyo, F., Fernandez, J. L., Arana Montes, María Del Pilar De, Johnston, S. D., and Gosalvez, J.

Abstract

The mixed success of equine artificial insemination programs using chilled and frozen-thawed semen is most likely associated with the variable response of the sperm cell to the preservation process and the fact that stallions are not selected on the basis of reproductive performance. We propose that the traditional indicators of sperm viability do not fully account for male factor infertility in the stallion and that knowledge of sperm DNA damage in the original semen sample and during semen processing may provide a more informed explanation of an individual stallion's reproductive potential. This study reports on the validation of a sperm DNA fragmentation test based on the sperm chromatin dispersion test (SCD) for stallion spermatozoa and on its application to semen that was chilled (4 °C; n = 10) or frozen-thawed (n = 13). Semen samples were collected by artificial vagina and the proportion of sperm with fragmented DNA determined. Seminal plasma was then removed by centrifugation and the sperm pellet re-suspended in commercial extenders prior to being chilled or cryopreserved using standard industry protocols. Chilled semen was cooled slowly to 4 °C and stored for 1 h before commencing the analysis; cryopreserved semen was thawed and immediately analyzed. Following chilling or cryopreservation, the semen samples were incubated at 37 °C and analyzed for SCD after 0, 4, 6, 24 and 48 h storage. The results of this investigation revealed that there was no significant difference in the sperm DNA fragmentation index (sDFI) of sperm evaluated initially after collection compared to those tested immediately after chilling or cryopreservation. However, within 1 h of incubation at 37 °C, both chilled and frozen-thawed spermatozoa showed a significant increase in the proportion of sDFI; after 6 h the sDFI had increased to over 50% and by 48 h, almost 100% of the sperm showed DNA damage. While the sDFI of individual stallions at equivalent times of incubation was variable, an, Ministerio de Educación y Ciencia, Depto. de Genética, Fisiología y Microbiología, Fac. de Ciencias Biológicas, TRUE, pub

Published
2024

9. Sperm DNA Fragmentation: A Critical Assessment of Clinical Practice Guidelines

Author

Agarwal, A. Farkouh, A. Parekh, N. Zini, A. Arafa, M. Kandil, H. Tadros, N. Busetto, G.M. Ambar, R. Parekattil, S. Boitrelle, F. Sallam, H. Jindal, S. Ko, E. Simopoulou, M. Park, H.J. Sadighi, M.A. Saleh, R. Ramsay, J. Martinez, M. Elbardisi, H. Alvarez, J. Colpi, G. Gosalvez, J. Evenson, D. Shah, R.

Abstract

Sperm DNA fragmentation (SDF) is implicated in male infertility and adverse reproductive outcomes. With the publication of many studies regarding the etiologies and contributors to SDF, as well as the effects of SDF, guidelines are necessary to aid clinicians in the application of SDF for male fertility evaluation. Two recent clinical practice guidelines were published by Agarwal et al and Esteves et al. In this article, we have evaluated and compared both guidelines. We have found fairly similar recommendations between the two guidelines and have also highlighted the differences between them. Finally, we have summarized and combined the best practice recommendations from both guidelines. Copyright © 2022 Korean Society for Sexual Medicine and Andrology

Published
2022

17. P–025 Sperm selection using a modified “swim up” technique in absence of sperm centrifugation improve sperm DNA fragmentation and decreases miscarriage rate

Author

Cabell. Vives, Y, primary, Belchin, P, additional, Lopez-Fernandez, C, additional, Fernandez-Rubio, M, additional, Guerrero-Sanchez, J, additional, Sanche. d. Burgos, M, additional, Garcia-Enguidanos, A, additional, Ordonez, D, additional, Izquierdo, E, additional, and Gosalvez, J, additional

Published
2021
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18. A comprehensive guide to sperm recovery in infertile men with retrograde ejaculation

Author

Gupta, S. Sharma, R. Agarwal, A. Parekh, N. Finelli, R. Shah, R. Kandil, H. Saleh, R. Arafa, M. Ko, E. Simopoulou, M. Zini, A. Rajmil, O. Kavoussi, P. Singh, K. Ambar, R.F. Elbardisi, H. Sengupta, P. Martinez, M. Boitrelle, F. Alves, M.G. Khalafalla, K. Roychoudhury, S. Busetto, G.M. Gosalvez, J. Tadros, N. Palani, A. Rodriguez, M.G. Anagnostopoulou, C. Micic, S. Rocco, L. Mostafa, T. Alvarez, J.G. Jindal, S. Sallam, H. Rosas, I.M. Lewis, S.E.M. AlSaid, S. Altan, M. Park, H.J. Ramsay, J. Parekattil, S. Sabbaghian, M. Tremellen, K. Vogiatzi, P. Gilani, M.A.S. Evenson, D.P. Colpi, G.M.

Abstract

Retrograde ejaculation (RE) is a condition defined as the backward flow of the semen during ejaculation, and when present can result in male infertility. RE may be partial or complete, resulting in either low seminal volume or complete absence of the ejaculate (dry ejaculate). RE can result from anatomic, neurological or pharmacological conditions. The treatment approaches outlined are determined by the cause. Alkalinizing urinary pH with oral medications or by adding sperm wash media into the bladder prior to ejaculation may preserve the viability of the sperm. This article provides a step-by-step guide to diagnose RE and the optimal techniques to retrieve sperm. Copyright © 2021 Korean Society for Sexual Medicine and Andrology

Published
2021

25. Male Oxidative Stress Infertility (MOSI): Proposed Terminology and Clinical Practice Guidelines for Management of Idiopathic Male Infertility

Author

Agarwal, A, Parekh, N, Selvam, MKP, Henkel, R, Shah, R, Homa, ST, Ramasamy, R, Ko, E, Tremellen, K, Esteves, S, Majzoub, A, Alvarez, JG, Gardner, DK, Jayasena, CN, Ramsay, JW, Cho, C-L, Saleh, R, Sakkas, D, Hotaling, JM, Lundy, SD, Vij, S, Marmar, J, Gosalvez, J, Sabanegh, E, Park, HJ, Zini, A, Kavoussi, P, Micic, S, Smith, R, Busetto, GM, Bakircioglu, ME, Haidl, G, Balercia, G, Garrido Puchalt, N, Ben-Khalifa, M, Tadros, N, Kirkman-Browne, J, Moskovtsev, S, Huang, X, Borges, E, Franken, D, Bar-Chama, N, Morimoto, Y, Tomita, K, Srini, VS, Ombelet, W, Baldi, E, Muratori, M, Yumura, Y, La Vignera, S, Kosgi, R, Martinez, MP, Evenson, DP, Zylbersztejn, DS, Roque, M, Cocuzza, M, Vieira, M, Ben-Meir, A, Orvieto, R, Levitas, E, Wiser, A, Arafa, M, Malhotra, V, Parekattil, SJ, Elbardisi, H, Carvalho, L, Dada, R, Sifer, C, Talwar, P, Gudeloglu, A, Mahmoud, AMA, Terras, K, Yazbeck, C, Nebojsa, B, Durairajanayagam, D, Mounir, A, Kahn, LG, Baskaran, S, Pai, RD, Paoli, D, Leisegang, K, Moein, M-R, Malik, S, Yaman, O, Samanta, L, Bayane, F, Jindal, SK, Kendirci, M, Altay, B, Perovic, D, Harlev, A, Agarwal, A, Parekh, N, Selvam, MKP, Henkel, R, Shah, R, Homa, ST, Ramasamy, R, Ko, E, Tremellen, K, Esteves, S, Majzoub, A, Alvarez, JG, Gardner, DK, Jayasena, CN, Ramsay, JW, Cho, C-L, Saleh, R, Sakkas, D, Hotaling, JM, Lundy, SD, Vij, S, Marmar, J, Gosalvez, J, Sabanegh, E, Park, HJ, Zini, A, Kavoussi, P, Micic, S, Smith, R, Busetto, GM, Bakircioglu, ME, Haidl, G, Balercia, G, Garrido Puchalt, N, Ben-Khalifa, M, Tadros, N, Kirkman-Browne, J, Moskovtsev, S, Huang, X, Borges, E, Franken, D, Bar-Chama, N, Morimoto, Y, Tomita, K, Srini, VS, Ombelet, W, Baldi, E, Muratori, M, Yumura, Y, La Vignera, S, Kosgi, R, Martinez, MP, Evenson, DP, Zylbersztejn, DS, Roque, M, Cocuzza, M, Vieira, M, Ben-Meir, A, Orvieto, R, Levitas, E, Wiser, A, Arafa, M, Malhotra, V, Parekattil, SJ, Elbardisi, H, Carvalho, L, Dada, R, Sifer, C, Talwar, P, Gudeloglu, A, Mahmoud, AMA, Terras, K, Yazbeck, C, Nebojsa, B, Durairajanayagam, D, Mounir, A, Kahn, LG, Baskaran, S, Pai, RD, Paoli, D, Leisegang, K, Moein, M-R, Malik, S, Yaman, O, Samanta, L, Bayane, F, Jindal, SK, Kendirci, M, Altay, B, Perovic, D, and Harlev, A

Abstract

Despite advances in the field of male reproductive health, idiopathic male infertility, in which a man has altered semen characteristics without an identifiable cause and there is no female factor infertility, remains a challenging condition to diagnose and manage. Increasing evidence suggests that oxidative stress (OS) plays an independent role in the etiology of male infertility, with 30% to 80% of infertile men having elevated seminal reactive oxygen species levels. OS can negatively affect fertility via a number of pathways, including interference with capacitation and possible damage to sperm membrane and DNA, which may impair the sperm's potential to fertilize an egg and develop into a healthy embryo. Adequate evaluation of male reproductive potential should therefore include an assessment of sperm OS. We propose the term Male Oxidative Stress Infertility, or MOSI, as a novel descriptor for infertile men with abnormal semen characteristics and OS, including many patients who were previously classified as having idiopathic male infertility. Oxidation-reduction potential (ORP) can be a useful clinical biomarker for the classification of MOSI, as it takes into account the levels of both oxidants and reductants (antioxidants). Current treatment protocols for OS, including the use of antioxidants, are not evidence-based and have the potential for complications and increased healthcare-related expenditures. Utilizing an easy, reproducible, and cost-effective test to measure ORP may provide a more targeted, reliable approach for administering antioxidant therapy while minimizing the risk of antioxidant overdose. With the increasing awareness and understanding of MOSI as a distinct male infertility diagnosis, future research endeavors can facilitate the development of evidence-based treatments that target its underlying cause.

Published
2019

45. Sau3A in situ digestion of human chromosome 3 pericentrometric heterochromatin. I. Differential digestion of alpha-satellite and satellite 1 DNA sequences

Author

Buno, I., Fernandez, J.L., Lopez-Fernandez, C., Diez-Martin, J.L., and Gosalvez, J.

Subjects
Haematology
Abstract

Item does not contain fulltext In situ digestion with the restriction endonuclease (RE) Sau3A (Sau3A REISD) uncovers a polymorphism for the pericentromeric heterochromatin of human chromosome 3, which can be positively stained (3+) or not (3-), and has proven useful to differentiate donor and recipient cells after sex-matched bone marrow transplantation and to analyze the so-called hemopoietic chimerism. The aim of the present investigation was to obtain insight into the molecular basis of such polymorphism to optimize its use for chimerism quantification using methodological approaches other than REISD. To this end, fluorescence in situ hybridization (FISH) assays using probes for the satellite DNA sequences that mainly constitute chromosome 3 pericentromeric heterochromatin (alpha-satellite and satellite 1 DNA) were performed on control and Sau3A-digested chromosomes. The results obtained suggest that chromosome 3 alpha-satellite DNA is digested in all individuals studied, irrespective of the karyotype obtained by Sau3A REISD (3++, 3+-, 3--), and thus it does not seem to be involved in the polymorphism uncovered by Sau3A on this chromosome. Satellite 1 DNA is not digested in any case, and shows a polymorphism for its domain size, which correlates with the polymorphism uncovered by Sau3A in such a way that 3+ chromosomes show a large domain (3L) and 3- chromosomes show a small domain (3S). It seems, therefore, that the cause of the polymorphism uncovered by Sau3A on the pericentromeric region of chromosome 3 is a difference in the size of the satellite 1 DNA domain. Small satellite 1 DNA domains fall under the resolution level of REISD technique and are identified as 3-.

Published
2001

48. Andrology

Author

Carchenilla, M. S. C., primary, Agudo, D., additional, Rubio, S., additional, Becerra, D., additional, Bronet, F., additional, Garcia-Velasco, J. A., additional, Pacheco, A., additional, Lardone, M., additional, Piottante, A., additional, Parada-Bustamante, A., additional, Argandona, F., additional, Florez, M., additional, Espinoza, A., additional, Ebensperger, M., additional, Castro, A., additional, Cohen-Bacrie, M., additional, Belloc, S., additional, Dalleac, A., additional, Amar, E., additional, Izard, V., additional, Hazout, A., additional, Cohen-Bacrie, P., additional, de Mouzon, J., additional, Muzzonigro, F., additional, Crivello, A. M., additional, Stanghellini, I., additional, Bernardini, L., additional, Ferraretti, A. P., additional, Magli, C., additional, Gianaroli, L., additional, Martin, P. S., additional, Duvison, M. H., additional, Silva, M. D., additional, Gosalvez, J., additional, Martin, F. S., additional, Pomante, A., additional, Colombo, F., additional, Mattioli, M., additional, Barboni, B., additional, Magli, M. C., additional, Hacifazlioglu, O., additional, Findikli, N., additional, Goktolga, U., additional, Bahceci, M., additional, Jakab, A., additional, Mokanszki, A., additional, Varga, A., additional, Benyo, M., additional, Kassai, Z., additional, Olah, E., additional, Molnar, Z., additional, Gundogan, G. I., additional, Bozkurt, H. H., additional, Irez, T., additional, Domingo, A., additional, Anarte, C., additional, Presilla, N., additional, Calvo, I., additional, Aguirre, O., additional, Oroquieta, A., additional, Agirregoikoa, J. A., additional, De Pablo, J. L., additional, Barrenetxea, G., additional, Moragues, I., additional, Medrano, M. L., additional, Montoya, A., additional, Ramos, B., additional, Torres, M. J. G., additional, Aizpurua, J., additional, Ibala, S. R., additional, Ghedir, H., additional, Mehri, A., additional, Zidi, I., additional, Brahem, S., additional, Mehdi, M., additional, Ajina, M., additional, Saad, A., additional, Gomez-Torres, M. J., additional, Cavaco, J. E., additional, Rato, L., additional, Alves, M. G., additional, Dias, T. R., additional, Lopes, G., additional, Socorro, S., additional, Oliveira, P. F., additional, Lobascio, A. M., additional, Minasi, M. G., additional, Greco, E., additional, Bungum, M., additional, Bungum, A., additional, Silver, N., additional, Zahiri, M., additional, Movahedin, M., additional, Mowla, S. J., additional, Noruzinia, M., additional, Huleihel, M., additional, Abarbanel, Y., additional, Haber, E. P., additional, Azab, M., additional, Lan, D., additional, Lunenfeld, E., additional, Smith, M. J., additional, Neri, Q. V., additional, Harvey, L., additional, Rosenwaks, Z., additional, Palermo, G. D., additional, Alhalabi, M., additional, Samawi, S., additional, Droubi, H., additional, Khalaf, M., additional, Taha, A., additional, Khatib, R., additional, Bednarowska-flisiak, A., additional, Wcislo, M., additional, Liss, J., additional, Swider, A., additional, Szczyglinska, J., additional, Grzymkowska, M., additional, Bruszczynska, A., additional, Glowacka, J., additional, Kitowska-Marszalkowska, K., additional, Krapchev, M., additional, Mirecka, A., additional, Wisniewska, K., additional, Lukaszuk, K., additional, Natali, I., additional, Tamburrino, L., additional, Cambi, M., additional, Marchiani, S., additional, Noci, I., additional, Maggi, M., additional, Forti, G., additional, Baldi, E., additional, Muratori, M., additional, Ferraretto, X., additional, Pasquet, B., additional, Damond, F., additional, Matheron, S., additional, Epelboin, S., additional, Yahi, S., additional, Demailly, P., additional, Rougier, N., additional, Yazbeck, C., additional, Delaroche, L., additional, Longuet, P., additional, Llabador, M., additional, Estellat, C., additional, Patrat, C., additional, Askarijahromi, M., additional, Amanlu, M., additional, Mowla, S. j., additional, Mazaheri, Z., additional, Christensen, P., additional, Sills, E. S., additional, Fischer, R., additional, Naether, O. G. J., additional, Walsh, D., additional, Rudolf, K., additional, Coull, G., additional, Baukloh, V., additional, Labouriau, R., additional, Birck, A., additional, Parisi, F., additional, Parrilla, B., additional, Oneta, M., additional, Savasi, V., additional, Veleva, L., additional, Milachich, T., additional, Bochev, I., additional, Antonova, I., additional, Shterev, A., additional, Vlaisavljevic, V., additional, Breznik, B. P., additional, Kovacic, B., additional, Serrano, M., additional, Gonzalvo, M. C., additional, Clavero, A., additional, Fernandez, M. F., additional, Mozas, J., additional, Martinez, L., additional, Fontes, J., additional, Carrillo, S., additional, Lopez-Regalado, M. L., additional, Lopez-Leria, B., additional, Orozco, I., additional, Mantilla, A., additional, Castilla, J. A., additional, Mskhalaya, G., additional, Zakharova, E., additional, Zaletova, V., additional, Kasatonova, E., additional, Melnik, Y., additional, Efremov, E., additional, Schiewe, M. C., additional, Verheyen, G., additional, Tournaye, H., additional, Phletincx, I., additional, Sims, C. A., additional, Rothman, C., additional, Borges, E., additional, Setti, A. S., additional, Braga, D. P. A. F., additional, Vingris, L., additional, Iaconelli, A., additional, Dupont, C., additional, Faure, C., additional, Sermondade, N., additional, Gautier, B., additional, Herbemont, C., additional, Aknin, I., additional, Klein, J. P., additional, Cedrin-Durnerin, I., additional, Wolf, J. P., additional, Czernichow, S., additional, Levy, R., additional, Rondanino, C., additional, Chauffour, C., additional, Ouchchane, L., additional, Artonne, C., additional, Janny, L., additional, Lobaccaro, J. M., additional, Volle, D. H., additional, Brugnon, F., additional, Colacurci, N., additional, Piomboni, P., additional, Ruvolo, G., additional, Lombardo, F., additional, Verde, E. L., additional, De Leo, V., additional, Lispi, M., additional, Papaleo, E., additional, De Palo, R., additional, Gandini, L., additional, Longobardi, S., additional, Yokota, Y., additional, Yokota, M., additional, Yokota, H., additional, Araki, Y., additional, Alshahrani, S., additional, Durairajanayagam, D., additional, Sharma, R., additional, Sabanegh, E., additional, Agarwal, A., additional, Hattori, H., additional, Nakajo, Y., additional, Ikeno, T., additional, Sato, Y., additional, Kyoya, T., additional, Kyono, K., additional, Li, B., additional, Li, J. B., additional, Xiao, X. F., additional, Ma, Y. F., additional, Wang, J., additional, Liang, X. X., additional, Zhao, H. X., additional, Jiang, F., additional, Yao, Y. Q., additional, Wang, X. H., additional, Roan, N. R., additional, Liu, H., additional, Muller, J., additional, Avila-Herrera, A., additional, Pollard, K. S., additional, Lishko, P., additional, Kirchhoff, F., additional, Munch, J., additional, Witkowska, H. E., additional, Greene, W. C., additional, Mangiarini, A., additional, Paffoni, A., additional, Restelli, L., additional, Guarneri, C., additional, Somigliana, E., additional, Ragni, G., additional, Bou, R., additional, Aleman, M., additional, Guardiola, F., additional, Camargo, C., additional, Oliveira, J. B. A., additional, Petersen, C. G., additional, Mauri, A. L., additional, Massaro, F. C., additional, Nicoletti, A., additional, Nascimento, A. M., additional, Vagnini, L. D., additional, Martins, A. M. V. C., additional, Cavagna, M., additional, Baruffi, R. L. R., additional, and Franco, J. G., additional

Published
2013
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50. 170 ASSESSMENT OF FRESH AND FROZEN - THAWED BOTTLENOSE DOLPHIN (TURSIOPS TRUNCATUS) SPERM DNA FRAGMENTATION USING THE SPERM CHROMATIN DISPERSION TEST

Author

Sanchez-Calabuig, M. J., primary, de la Fuente, J., additional, Beltrán-Breña, P., additional, Martinez-Nevado, E., additional, Perez-Gutierrez, J. F., additional, Blyde, D., additional, Johnston, S., additional, Harrison, K., additional, Lopez-Fernandez, C., additional, and Gosalvez, J., additional

Published
2013
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