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1. Specific serum antibody responses following a Toxoplasma gondii and Trichinella spiralis co-infection in swine

Author

Bokken, G.C.A.M., van Eerden, E., Opsteegh, M., Augustijn, M., Graat, E.A.M., Franssen, F.F.J., Gorlich, K., Buschtöns, S., Tenter, A.M., van der Giessen, J.W.B., Bergwerff, A.A., van Knapen, F., Advances in Veterinary Medicine, Risk Assessment of Toxic and Immunomodulatory Agents, Dep IRAS, Advances in Veterinary Medicine, Risk Assessment of Toxic and Immunomodulatory Agents, and Dep IRAS

Subjects
mice, Time Factors, Serial dilution, diagnosis, Swine, Kwantitatieve Veterinaire Epidemiologie, Trichinella spiralis, pork, Antibodies, Helminth, Antibodies, Protozoan, netherlands, Artificial digestion, Mice, parasitic diseases, Animals, Parasite hosting, infections, Swine Diseases, General Veterinary, biology, Coinfection, Inoculation, britovi, Quantitative Veterinary Epidemiology, pigs, Toxoplasma gondii, Trichinellosis, tissue cysts, General Medicine, biology.organism_classification, Virology, Toxoplasmosis, Animal, International (English), Antibody Formation, WIAS, biology.protein, Female, Parasitology, Antibody, Toxoplasma, Co infection
Abstract

The aim of this study was to examine the dynamics of parasite specific antibody development in Trichinella spiralis and Toxoplasma gondii co-infections in pigs and to compare these with antibody dynamics in T. spiralis and T. gondii single infections. In this experiment, fifty-four pigs were divided into five inoculated groups of ten animals, and one control group of four animals. Two groups were inoculated with a single dose of either T. gondii tissue cysts or T. spiralis muscle larvae, one group was inoculated simultaneously with both parasites and two groups were successively inoculated at an interval of four weeks. Specific IgG responses to the parasites were measured by ELISA. T. gondii burden was determined by MC-PCR carried out on heart muscle and T. spiralis burden by artificial digestion of diaphragm samples. Specific IgG responses to T. gondii and T. spiralis in single and simultaneously inoculated animals showed a respective T. gondii and T. spiralis inoculation effect but no significant interaction of these parasites to the development of specific antibodies with the serum dilutions used. Moreover, our data showed that the specific IgG response levels in groups of animals successively or simultaneously co-infected were independent of a respective previous or simultaneous infection with the other parasite. Additionally, no differences in parasite burden were found within groups inoculated with T. gondii and within groups inoculated with T. spiralis. Conclusively, for the infection doses tested in this experiment, the dynamics of specific antibody development does not differ between single and simultaneous or successive infection with T. gondii and T. spiralis. However, lower parasitic doses and other ratios of doses, like low-low, low-high and high-low of T. gondii and T. spiralis in co-infection, in combination with other time intervals between successive infections may have different outcomes and should therefore be studied in further detail.

Published
2012

2. Specific serum antibody responses following a Toxoplasma gondii and Trichinella spiralis co-infection in swine

Author

Advances in Veterinary Medicine, Risk Assessment of Toxic and Immunomodulatory Agents, Dep IRAS, Bokken, G.C.A.M., van Eerden, E., Opsteegh, M., Augustijn, M., Graat, E.A.M., Franssen, F.F.J., Gorlich, K., Buschtöns, S., Tenter, A.M., van der Giessen, J.W.B., Bergwerff, A.A., van Knapen, F., Advances in Veterinary Medicine, Risk Assessment of Toxic and Immunomodulatory Agents, Dep IRAS, Bokken, G.C.A.M., van Eerden, E., Opsteegh, M., Augustijn, M., Graat, E.A.M., Franssen, F.F.J., Gorlich, K., Buschtöns, S., Tenter, A.M., van der Giessen, J.W.B., Bergwerff, A.A., and van Knapen, F.

Published
2012

5. Prognostic value of minimal residual disease quantification by real-time reverse transcriptase polymerase chain reaction in patients with core binding factor leukemias.

Author

Krauter J, Gorlich K, Ottmann O, Lubbert M, Dohner H, Heit W, Kanz L, Ganser A, and Heil G

Subjects
Adolescent, Adult, Chromosome Inversion, Chromosomes, Human, Pair 16 genetics, Chromosomes, Human, Pair 21 genetics, Chromosomes, Human, Pair 8 genetics, Core Binding Factor Alpha 2 Subunit, DNA Primers chemistry, Female, Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) genetics, Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) metabolism, Humans, Leukemia, Myeloid, Acute mortality, Male, Middle Aged, Neoplasm, Residual, Oncogene Proteins, Fusion metabolism, Prognosis, RNA, Messenger genetics, RNA, Messenger metabolism, RNA, Neoplasm analysis, RUNX1 Translocation Partner 1 Protein, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Survival Rate, Transcription Factors metabolism, Translocation, Genetic, Leukemia, Myeloid, Acute diagnosis, Leukemia, Myeloid, Acute genetics, Oncogene Proteins, Fusion genetics, Transcription Factors genetics
Abstract

Purpose: In patients with acute myeloblastic leukemia with t(8;21) or inv(16) aberrations (core binding factor [CBF] leukemias), minimal residual disease (MRD) can be sensitively detected during and after chemotherapy by use of molecular methods. However, the prognostic impact of qualitative MRD detection is still under debate. In this study, the prognostic value of MRD quantification in patients with CBF leukemias was assessed., Patients and Methods: We quantified MRD at various time points during and after therapy by real-time reverse transcriptase polymerase chain reaction (RT-PCR) for AML1/MTG8 and CBFB/MYH11 in 37 patients with CBF leukemias treated within a multicenter trial., Results: At initial diagnosis, the patients showed a heterogenous fusion gene expression relative to glyceraldehyde 3-phosphate dehydrogenase with a variation of more than two log steps. According to MRD status during/after therapy, two groups of patients were separated. Of the 26 patients who had MRD levels of less than 1% in relation to initial diagnosis at all time points tested after induction chemotherapy, only two experienced relapse after a median follow-up of 19 months. Of the 11 patients who had a sample with an MRD level >/= 1% at least at one time point after induction therapy, 10 experienced relapse, with a median remission duration of 10 months (P <.001). The median interval between the informative MRD sample and clinical relapse in these patients was 3 months., Conclusion: MRD quantification by real-time RT-PCR allows the identification of patients with a high risk of relapse among the CBF leukemias.

Published
2003
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