1. Comparison of dried blood spot and plasma sampling for untargeted metabolomics
- Author
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Tobin, Nicole H, Murphy, Aisling, Li, Fan, Brummel, Sean S, Taha, Taha E, Saidi, Friday, Owor, Maxie, Violari, Avy, Moodley, Dhayendre, Chi, Benjamin, Goodman, Kelli D, Koos, Brian, and Aldrovandi, Grace M
- Subjects
Medical Biochemistry and Metabolomics ,Analytical Chemistry ,Biomedical and Clinical Sciences ,Chemical Sciences ,Clinical Research ,Biomarkers ,Case-Control Studies ,Dried Blood Spot Testing ,Female ,Humans ,Metabolomics ,Pregnancy ,Specimen Handling ,Plasma ,Dried blood spots ,Comparison ,Biochemistry and Cell Biology ,Clinical Sciences ,Biochemistry and cell biology ,Medical biochemistry and metabolomics ,Analytical chemistry - Abstract
IntroductionUntargeted metabolomics holds significant promise for biomarker detection and development. In resource-limited settings, a dried blood spot (DBS)-based platform would offer significant advantages over plasma-based approaches that require a cold supply chain.ObjectivesThe primary goal of this study was to compare the ability of DBS- and plasma-based assays to characterize maternal metabolites. Utility of the two assays was also assessed in the context of a case-control predictive model in pregnant women living with HIV.MethodsUntargeted metabolomics was performed on archived paired maternal plasma and DBS from n = 79 women enrolled in a large clinical trial.ResultsA total of 984 named biochemicals were detected across both plasma and DBS samples, of which 627 (63.7%), 260 (26.4%), and 97 (9.9%) were detected in both plasma and DBS, plasma alone, and DBS alone, respectively. Variation attributable to study individual (R2 = 0.54, p
- Published
- 2021